orbitrap fusion lumos mass spectrometer  (Thermo Fisher)


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    Thermo Fisher orbitrap fusion lumos mass spectrometer
    Orbitrap Fusion Lumos Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orbitrap fusion lumos mass spectrometer/product/Thermo Fisher
    Average 98 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    orbitrap fusion lumos mass spectrometer - by Bioz Stars, 2020-05
    98/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Boosting the Biogenesis and Secretion of Mesenchymal Stem Cell-Derived Exosomes
    Article Snippet: .. Peptides were injected onto a 30-cm C18 column with 1.8 µm beads (Sepax, Newark, NJ, USA), with an Easy nanoLC-1200 HPLC (Thermo Fisher, Waltham, MA, USA), connected to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher). ..

    Injection:

    Article Title: STK25 suppresses Hippo signaling by regulating SAV1-STRIPAK antagonism
    Article Snippet: .. The sample then underwent solid-phase extraction cleanup with an Oasis HLB plate (Waters), and the resulting sample was injected onto an Orbitrap Fusion Lumos mass spectrometer (Thermo Electron) coupled to an Ultimate 3000 RSLC-Nano liquid chromatography system (Dionex). .. Raw MS data file was analyzed using Proteome Discoverer v 2.2 (Thermo), with peptide identification performed using Sequest HT searching against the human protein database from UniProt.

    Article Title: Boosting the Biogenesis and Secretion of Mesenchymal Stem Cell-Derived Exosomes
    Article Snippet: .. Peptides were injected onto a 30-cm C18 column with 1.8 µm beads (Sepax, Newark, NJ, USA), with an Easy nanoLC-1200 HPLC (Thermo Fisher, Waltham, MA, USA), connected to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher). ..

    Liquid Chromatography:

    Article Title: STK25 suppresses Hippo signaling by regulating SAV1-STRIPAK antagonism
    Article Snippet: .. The sample then underwent solid-phase extraction cleanup with an Oasis HLB plate (Waters), and the resulting sample was injected onto an Orbitrap Fusion Lumos mass spectrometer (Thermo Electron) coupled to an Ultimate 3000 RSLC-Nano liquid chromatography system (Dionex). .. Raw MS data file was analyzed using Proteome Discoverer v 2.2 (Thermo), with peptide identification performed using Sequest HT searching against the human protein database from UniProt.

    Article Title: Acute unfolding of a single protein immediately stimulates recruitment of ubiquitin protein ligase E3C (UBE3C) to 26S proteasomes
    Article Snippet: .. Mass spectrometry data were collected using an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA) coupled to a Proxeon EASY-nLC1200 liquid chromatography (LC) pump (Thermo Fisher Scientific). .. Peptides were separated on a 100-μm inner diameter microcapillary column packed in-house with ∼35 cm of Accucore150 resin (2.6 μm, 150 Å, Thermo Fisher Scientific) with a gradient consisting of 5–26% (0–155 min), 26–32% (155–170 min) (acetonitrile, 0.1% formic acid) over a total 180-min run at ∼600 nl/min.

    Mass Spectrometry:

    Article Title: STK25 suppresses Hippo signaling by regulating SAV1-STRIPAK antagonism
    Article Snippet: .. The sample then underwent solid-phase extraction cleanup with an Oasis HLB plate (Waters), and the resulting sample was injected onto an Orbitrap Fusion Lumos mass spectrometer (Thermo Electron) coupled to an Ultimate 3000 RSLC-Nano liquid chromatography system (Dionex). .. Raw MS data file was analyzed using Proteome Discoverer v 2.2 (Thermo), with peptide identification performed using Sequest HT searching against the human protein database from UniProt.

    Article Title: Irisin Mediates Effects on Bone and Fat via αV Integrin Receptors
    Article Snippet: .. All LC-MS/MS experiments were performed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) coupled with a Proxeon EASY-nLC 1200 LC pump (Thermo Fisher Scientific). .. Peptides were separated on a 100µm inner diameter microcapillary column packed with 35cm of Accucore C18 resin (1.8µm, 100Å, Thermo Fisher Scientific).

    Article Title: Exosomes from Human Adipose Tissue-Derived Mesenchymal Stem Cells Promote Epidermal Barrier Repair by Inducing de Novo Synthesis of Ceramides in Atopic Dermatitis
    Article Snippet: .. Peptides were analyzed using an LC-MS/MS system consisting of an Easy nLC 1000 (Thermo Fisher Scientific) and an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) equipped with a nano-electrospray source (EASY-Spray Sources, Thermo Fisher Scientific). .. Peptides were trapped in a 75 μm × 2 cm C18 precolumn (nanoViper, Acclaim PepMap100, Thermo Fisher Scientific) before being separated on an analytical C18 column (75 μm × 50 cm PepMap RSLC, Thermo Fisher Scientific) at a flow rate of 300 nL/min.

    Article Title: Direct Ultraviolet Laser-Induced Reduction of Disulfide Bonds in Insulin and Vasopressin
    Article Snippet: .. Sample Analysis For LC–MS1 and −MS2 analyses, a Vanquish Horizon UHPLC system with a VF-D40 detector was coupled to an Orbitrap Fusion Lumos mass spectrometer (both from Thermo Fisher Scientific, CA). .. For online absorption spectroscopy, an Acquity UPLC Classic system with an Acquity photodiode array was used, which was coupled to a Synapt G2Si mass spectrometer (both from Waters, U.K.).

    Article Title: Time-resolved proteome profiling of normal lung development
    Article Snippet: .. A sample (5 μl) of 0.1 μg/μl of peptides from each fraction was analyzed by reverse-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a Waters nanoEquityTM UPLC system (Millford) coupled with a Orbitrap Fusion Lumos mass spectrometer (ThermoScientific) as described below. .. Analytical columns were made in house by slurry packing 3-μm Jupiter C18 stationary phase (Phenomenex) into a 70-cm-long, 360-μm OD × 75-μm ID fused silica capillary tubing (Polymicro Technologies).

    Article Title: Larval Geoduck (Panopea generosa) Proteomic Response to Ciliates
    Article Snippet: .. Larval peptides were analyzed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific, Waltham, MA, USA) with a 4.5 cm, 100 µm pre-column and a 26 cm, 75 µm analytical column, both packed in-house with 3 µm C18 Dr. Maisch (Germany) beads. .. The analytical column was housed in a 50 °C column heater for the duration of the analysis to improve chromatography.

    Article Title: Acute unfolding of a single protein immediately stimulates recruitment of ubiquitin protein ligase E3C (UBE3C) to 26S proteasomes
    Article Snippet: .. Mass spectrometry data were collected using an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA) coupled to a Proxeon EASY-nLC1200 liquid chromatography (LC) pump (Thermo Fisher Scientific). .. Peptides were separated on a 100-μm inner diameter microcapillary column packed in-house with ∼35 cm of Accucore150 resin (2.6 μm, 150 Å, Thermo Fisher Scientific) with a gradient consisting of 5–26% (0–155 min), 26–32% (155–170 min) (acetonitrile, 0.1% formic acid) over a total 180-min run at ∼600 nl/min.

    Article Title: Boosting the Biogenesis and Secretion of Mesenchymal Stem Cell-Derived Exosomes
    Article Snippet: .. Peptides were injected onto a 30-cm C18 column with 1.8 µm beads (Sepax, Newark, NJ, USA), with an Easy nanoLC-1200 HPLC (Thermo Fisher, Waltham, MA, USA), connected to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher). ..

    Chromatography:

    Article Title: Time-resolved proteome profiling of normal lung development
    Article Snippet: .. A sample (5 μl) of 0.1 μg/μl of peptides from each fraction was analyzed by reverse-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a Waters nanoEquityTM UPLC system (Millford) coupled with a Orbitrap Fusion Lumos mass spectrometer (ThermoScientific) as described below. .. Analytical columns were made in house by slurry packing 3-μm Jupiter C18 stationary phase (Phenomenex) into a 70-cm-long, 360-μm OD × 75-μm ID fused silica capillary tubing (Polymicro Technologies).

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  • 99
    Thermo Fisher orbitrap fusion lumos mass spectrometer
    Gas phase segmentation (GPS) improves ADPr peptide detection. (A) A screenshot of the ADP-ribose product ion triggered EThcD and HCD data acquisition method on the <t>Orbitrap</t> Fusion <t>Lumos.</t> (B) Area under the curve (AUC) of the four ADPr fragment ions (136.06, 250.09, 348.07, and 428.04 m / z ) dissociated from an ARTD8/PARP14-MARylated STAT1 peptide using HCD. (C) Pilot Af1521 enrichment study to determine the optimal collision energy for ADP-ribose product ion screening and ADPr peptide identification. Only high confidence (HCD and EThcD combined peptides) were used in this plot (more details in Figure 3 ). (D) A schematic showing the principle of GPS using multiple injections. (E) The extracted ion chromatograms of the ADPr fragment peak (348.07 m / z ) in each full scan and GPS injection. (F,G) Precursor ion m / z and retention time of triggered EThcD spectra in full scan and combined GPS scans.
    Orbitrap Fusion Lumos Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/orbitrap fusion lumos mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 85 article reviews
    Price from $9.99 to $1999.99
    orbitrap fusion lumos mass spectrometer - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

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    Gas phase segmentation (GPS) improves ADPr peptide detection. (A) A screenshot of the ADP-ribose product ion triggered EThcD and HCD data acquisition method on the Orbitrap Fusion Lumos. (B) Area under the curve (AUC) of the four ADPr fragment ions (136.06, 250.09, 348.07, and 428.04 m / z ) dissociated from an ARTD8/PARP14-MARylated STAT1 peptide using HCD. (C) Pilot Af1521 enrichment study to determine the optimal collision energy for ADP-ribose product ion screening and ADPr peptide identification. Only high confidence (HCD and EThcD combined peptides) were used in this plot (more details in Figure 3 ). (D) A schematic showing the principle of GPS using multiple injections. (E) The extracted ion chromatograms of the ADPr fragment peak (348.07 m / z ) in each full scan and GPS injection. (F,G) Precursor ion m / z and retention time of triggered EThcD spectra in full scan and combined GPS scans.

    Journal: Journal of Proteome Research

    Article Title: A Study into the ADP-Ribosylome of IFN-γ-Stimulated THP-1 Human Macrophage-like Cells Identifies ARTD8/PARP14 and ARTD9/PARP9 ADP-Ribosylation

    doi: 10.1021/acs.jproteome.8b00895

    Figure Lengend Snippet: Gas phase segmentation (GPS) improves ADPr peptide detection. (A) A screenshot of the ADP-ribose product ion triggered EThcD and HCD data acquisition method on the Orbitrap Fusion Lumos. (B) Area under the curve (AUC) of the four ADPr fragment ions (136.06, 250.09, 348.07, and 428.04 m / z ) dissociated from an ARTD8/PARP14-MARylated STAT1 peptide using HCD. (C) Pilot Af1521 enrichment study to determine the optimal collision energy for ADP-ribose product ion screening and ADPr peptide identification. Only high confidence (HCD and EThcD combined peptides) were used in this plot (more details in Figure 3 ). (D) A schematic showing the principle of GPS using multiple injections. (E) The extracted ion chromatograms of the ADPr fragment peak (348.07 m / z ) in each full scan and GPS injection. (F,G) Precursor ion m / z and retention time of triggered EThcD spectra in full scan and combined GPS scans.

    Article Snippet: LC–MS/MS Analysis All peptide samples were analyzed on an Orbitrap Fusion Lumos mass spectrometer fronted with an EASY-Spray Source, coupled to an Easy-nLC1000 HPLC pump (Thermo Fisher Scientific).

    Techniques: Injection

    DiLeu cPILOT experimental workflow. Protein digest samples undergo stable isotope dimethyl labeling of peptide N-termini with either light [–(CH 3 ) 2 ] or heavy [–( 13 C 2 H 3 ) 2 ] dimethyl groups at low pH followed by labeling of lysine residues with 12-plex DiLeu isobaric tags at high pH. Pooled samples are analyzed by LC-MS/MS on the Orbitrap Fusion Lumos using CID MS 2 acquisition of peak pairs for peptide sequence identification and HCD SPS-MS 3 acquisition for accurate 24-plex quantification via DiLeu reporter ions.

    Journal: Analytical chemistry

    Article Title: Increased N,N-Dimethyl Leucine Isobaric Tag Multiplexing by a Combined Precursor Isotopic Labeling and Isobaric Tagging Approach

    doi: 10.1021/acs.analchem.8b01301

    Figure Lengend Snippet: DiLeu cPILOT experimental workflow. Protein digest samples undergo stable isotope dimethyl labeling of peptide N-termini with either light [–(CH 3 ) 2 ] or heavy [–( 13 C 2 H 3 ) 2 ] dimethyl groups at low pH followed by labeling of lysine residues with 12-plex DiLeu isobaric tags at high pH. Pooled samples are analyzed by LC-MS/MS on the Orbitrap Fusion Lumos using CID MS 2 acquisition of peak pairs for peptide sequence identification and HCD SPS-MS 3 acquisition for accurate 24-plex quantification via DiLeu reporter ions.

    Article Snippet: Samples were analyzed by nanoLC-MS/MS using a Dionex UltiMate 3000 UPLC system coupled to a Thermo Scientific Orbitrap Fusion Lumos mass spectrometer.

    Techniques: Labeling, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Sequencing

    General sample preparation and data acquisition workflow: N-glycans were released by FANGS, labeled with 6-plex aminoxyTMT, combined and cleaned up with an Oasis HLB cartridge, and analyzed on an LC-Tribrid quadrupole-ion trap-Orbitrap MS platform.

    Journal: Analytical chemistry

    Article Title: Targeted MultiNotch MS3 Approach for Relative Quantification of N-Glycans Using Multiplexed Carbonyl-Reactive Isobaric Tags

    doi: 10.1021/acs.analchem.7b03289

    Figure Lengend Snippet: General sample preparation and data acquisition workflow: N-glycans were released by FANGS, labeled with 6-plex aminoxyTMT, combined and cleaned up with an Oasis HLB cartridge, and analyzed on an LC-Tribrid quadrupole-ion trap-Orbitrap MS platform.

    Article Snippet: An Orbitrap Fusion Lumos Tribrid quadrupole-ion trap-Orbitrap mass spectrometer with NanoSpray Flex ion source (Thermo Scientific, Bremen, Germany) was used for data acquisition.

    Techniques: Sample Prep, Labeling, Mass Spectrometry

    Cluster analysis of validated seven differential proteins by different mass spectrometers. Cluster analysis of differential proteins by Triple TOF 5600 ( a ) and by Orbitrap Fusion Lumos ( b ).

    Journal: Scientific Reports

    Article Title: Early urine proteome changes in the Walker-256 tail-vein injection rat model

    doi: 10.1038/s41598-019-50301-1

    Figure Lengend Snippet: Cluster analysis of validated seven differential proteins by different mass spectrometers. Cluster analysis of differential proteins by Triple TOF 5600 ( a ) and by Orbitrap Fusion Lumos ( b ).

    Article Snippet: PRM samples were analyzed by the EASY-nLC 1200 HPLC system (Thermo Fisher Scientific, Waltham, MA) coupled to the Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA).

    Techniques: