Journal: Clinical and Vaccine Immunology : CVI

Article Title: Distinct Effects of Anti-Tumor Necrosis Factor Combined Therapy on TH1/TH2 Balance in Rheumatoid Arthritis Patients ^▿

doi: 10.1128/CVI.00061-11

Figure Lengend Snippet: Fluorescence and bright-field images of lymphocytes settled within the LiveCell array. Shown are an overlapping image of the fluorescence and bright-field images (left) and fluorescence images (right) of the same cell population double stained for the expression of CD4 receptors (A) and IL-12R (B).

Article Snippet: After 10 min of incubation in the dark at room temperature, cells were analyzed either by imaging using the Optical LiveCell Array (LCA; Molecular Cytomics, Boston, MA) for individual cell measurements or by FACS flow cytometry for population measurements (FACSCalibur; Becton-Dickinson, Mountainview, CA).

Techniques: Fluorescence, Staining, Expressing

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Distinct Effects of Anti-Tumor Necrosis Factor Combined Therapy on TH1/TH2 Balance in Rheumatoid Arthritis Patients ^▿

doi: 10.1128/CVI.00061-11

Figure Lengend Snippet: Fluorescence and bright-field images of lymphocytes settled within the LiveCell array. Shown are an overlapping image of the fluorescence and bright-field images (left) and fluorescence images (right) of the same cell population double stained for the expression of CD4 receptors (A) and IL-12R (B).

Article Snippet: Cells were introduced into the Optical LiveCell Array (Molecular Cytomics, Boston, MA), a densely packed hexagonal transparent array of micron-sized wells which hold individual cells in suspension.

Techniques: Fluorescence, Staining, Expressing