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  • 93
    Name:
    OPG h PR
    Description:
    Gene Silencers generally consist of pools of three to five target specific 19 25 nucleotide sequences in length For independent verification of OPG gene silencing results individual duplex components or plasmids are also available upon request Suitable control antibody OPG Antibody E 10 sc 390518 is recommended as control antibody for monitoring of OPG expression knockdown by Western blotting or immunofluorescence
    Catalog Number:
    SC-40152-PR
    Price:
    None
    Category:
    Gene Editing siRNA shRNA Gene Silencers Membrane Receptor OPG siRNA shRNA Plasmid and Lentiviral Particle Gene Silencers OPG siRNA and shRNA Plasmids h
    Buy from Supplier


    Structured Review

    Santa Cruz Biotechnology opg
    LLDT-8 increased the ratio of <t>OPG/RANKL</t> in synovial fluid of RA patients. SFMCs were isolated from synovial fluid of RA patients and treated with various concentrations of LLDT-8 (0, 25 and 50 nM, respectively) for 24 hours, then stained and detected by flow cytometry. (A) The psuedocolor plots of OPG, RANK and RANKL. (B) The rate of OPG expression on <t>CD3</t> + T leukomonocyte in the three groups. (C) The ratio of OPG/RANKL on CD3 + T cells in the three groups. * p
    Gene Silencers generally consist of pools of three to five target specific 19 25 nucleotide sequences in length For independent verification of OPG gene silencing results individual duplex components or plasmids are also available upon request Suitable control antibody OPG Antibody E 10 sc 390518 is recommended as control antibody for monitoring of OPG expression knockdown by Western blotting or immunofluorescence
    https://www.bioz.com/result/opg/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    opg - by Bioz Stars, 2021-07
    93/100 stars

    Images

    1) Product Images from "(5R)-5-Hydroxytriptolide (LLDT-8) inhibits osteoclastogenesis via RANKL/RANK/OPG signaling pathway"

    Article Title: (5R)-5-Hydroxytriptolide (LLDT-8) inhibits osteoclastogenesis via RANKL/RANK/OPG signaling pathway

    Journal: BMC Complementary and Alternative Medicine

    doi: 10.1186/s12906-015-0566-y

    LLDT-8 increased the ratio of OPG/RANKL in synovial fluid of RA patients. SFMCs were isolated from synovial fluid of RA patients and treated with various concentrations of LLDT-8 (0, 25 and 50 nM, respectively) for 24 hours, then stained and detected by flow cytometry. (A) The psuedocolor plots of OPG, RANK and RANKL. (B) The rate of OPG expression on CD3 + T leukomonocyte in the three groups. (C) The ratio of OPG/RANKL on CD3 + T cells in the three groups. * p
    Figure Legend Snippet: LLDT-8 increased the ratio of OPG/RANKL in synovial fluid of RA patients. SFMCs were isolated from synovial fluid of RA patients and treated with various concentrations of LLDT-8 (0, 25 and 50 nM, respectively) for 24 hours, then stained and detected by flow cytometry. (A) The psuedocolor plots of OPG, RANK and RANKL. (B) The rate of OPG expression on CD3 + T leukomonocyte in the three groups. (C) The ratio of OPG/RANKL on CD3 + T cells in the three groups. * p

    Techniques Used: Isolation, Staining, Flow Cytometry, Cytometry, Expressing

    LLDT-8 up-regulated OPG expression and increased the ratio of OPG/RANKL on CD3 + T leukomonocyte in peripheral blood of RA patients. PBMCs were isolated from peripheral blood of RA patients and treated with various concentrations of LLDT-8 (0, 25 nM and 50 nM, respectively) for 24 hours, then stained and detected by flow cytometry. (A) The psuedocolor plots of OPG, RANK and RANKL. (B) The rate of OPG expression on CD3 + T leukomonocyte in the three groups. (C) The ratio of OPG to RANKL on CD3 + T leukomonocyte in the three groups. * p
    Figure Legend Snippet: LLDT-8 up-regulated OPG expression and increased the ratio of OPG/RANKL on CD3 + T leukomonocyte in peripheral blood of RA patients. PBMCs were isolated from peripheral blood of RA patients and treated with various concentrations of LLDT-8 (0, 25 nM and 50 nM, respectively) for 24 hours, then stained and detected by flow cytometry. (A) The psuedocolor plots of OPG, RANK and RANKL. (B) The rate of OPG expression on CD3 + T leukomonocyte in the three groups. (C) The ratio of OPG to RANKL on CD3 + T leukomonocyte in the three groups. * p

    Techniques Used: Expressing, Isolation, Staining, Flow Cytometry, Cytometry

    2) Product Images from "Zinc Sulfate Stimulates Osteogenic Phenotypes in Periosteum-Derived Cells and Co-Cultures of Periosteum-Derived Cells and THP-1 Cells"

    Article Title: Zinc Sulfate Stimulates Osteogenic Phenotypes in Periosteum-Derived Cells and Co-Cultures of Periosteum-Derived Cells and THP-1 Cells

    Journal: Life

    doi: 10.3390/life11050410

    Effects of zinc sulfate on the levels of RANKL and OPG and the RANKL/OPG ratio in co-cultured h PDCs and THP-1 cells. ( A ) Protein levels of RANKL and OPG in co-cultured h PDCs and THP-1 cells treated with or without zinc sulfate after 5, 10, and 15 days of culture. ( B ) Measurement of the RANK/OPG ratio in co-cultured cells treated with or without zinc sulfate after 5, 10, and 15 days in culture. ** p
    Figure Legend Snippet: Effects of zinc sulfate on the levels of RANKL and OPG and the RANKL/OPG ratio in co-cultured h PDCs and THP-1 cells. ( A ) Protein levels of RANKL and OPG in co-cultured h PDCs and THP-1 cells treated with or without zinc sulfate after 5, 10, and 15 days of culture. ( B ) Measurement of the RANK/OPG ratio in co-cultured cells treated with or without zinc sulfate after 5, 10, and 15 days in culture. ** p

    Techniques Used: Cell Culture

    3) Product Images from "Icariin Prevents Cartilage and Bone Degradation in Experimental Models of Arthritis"

    Article Title: Icariin Prevents Cartilage and Bone Degradation in Experimental Models of Arthritis

    Journal: Mediators of Inflammation

    doi: 10.1155/2016/9529630

    Decreased RANKL and enhanced OPG expression in articular cartilage is associated with icariin therapy. Icariin treatment reduced RANKL and enhanced OPG protein and mRNA expression in subchondral bone as shown by immunohistochemistry (a) and real-time PCR analysis (b, c). RANKL/OPG ratio (d). Results are presented as the mean ± SD. ∗ p
    Figure Legend Snippet: Decreased RANKL and enhanced OPG expression in articular cartilage is associated with icariin therapy. Icariin treatment reduced RANKL and enhanced OPG protein and mRNA expression in subchondral bone as shown by immunohistochemistry (a) and real-time PCR analysis (b, c). RANKL/OPG ratio (d). Results are presented as the mean ± SD. ∗ p

    Techniques Used: Expressing, Immunohistochemistry, Real-time Polymerase Chain Reaction

    4) Product Images from "Angiotensin (1-7) ameliorates the structural and biochemical alterations of ovariectomy-induced osteoporosis in rats via activation of ACE-2/Mas receptor axis"

    Article Title: Angiotensin (1-7) ameliorates the structural and biochemical alterations of ovariectomy-induced osteoporosis in rats via activation of ACE-2/Mas receptor axis

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-02570-x

    Effects of Ang(1-7) and/or A-779 treatments to the sham and OVX animals for 6 weeks on the expressions of RAS different proteins and osteoclastogenesis modulating factors in the femoral bone heads. ( A ) Western blot analysis bands showing the expressions of AngII, Ang(1-7), AT1R, AT2R, ACE, ACE-2, MasR, RANKL and OPG. ( B ) Quantification of the scanning densitometry of the western blot bands (n = 8/group) expressed as arbitrary units. One-way ANOVA test followed by post hoc Student-Newman-Keuls multiple comparisons test were used for the statistical analysis. Columns and bars represent the mean ± SEM of each group. Statistical significance was considered when *P
    Figure Legend Snippet: Effects of Ang(1-7) and/or A-779 treatments to the sham and OVX animals for 6 weeks on the expressions of RAS different proteins and osteoclastogenesis modulating factors in the femoral bone heads. ( A ) Western blot analysis bands showing the expressions of AngII, Ang(1-7), AT1R, AT2R, ACE, ACE-2, MasR, RANKL and OPG. ( B ) Quantification of the scanning densitometry of the western blot bands (n = 8/group) expressed as arbitrary units. One-way ANOVA test followed by post hoc Student-Newman-Keuls multiple comparisons test were used for the statistical analysis. Columns and bars represent the mean ± SEM of each group. Statistical significance was considered when *P

    Techniques Used: Western Blot

    5) Product Images from "RANK- and c-Met-mediated signal network promotes prostate cancer metastatic colonization"

    Article Title: RANK- and c-Met-mediated signal network promotes prostate cancer metastatic colonization

    Journal: Endocrine-Related Cancer

    doi: 10.1530/ERC-13-0548

    RANKL induces expression of RANKL and c-Met but suppresses AR expression/activation through c-Myc/Max and AP4 transcription factors in the LNCaP cell background. (A) RANKL overexpression upregulates endogenous RANKL and c-Met expression and c-Met phosphorylation but suppresses AR expression. (B) Exogenous RANKL treatment or intrinsic RANKL expression induces endogenous RANKL expression, c-Met expression, and c-Met phosphorylation. Inductions are partly sensitive to OPG or anti-RANKL antibody (denosumab) treatments. (C) RANKL–RANK signaling increases the level of c-Myc and Max expression. Increased c-Myc and Max nuclear proteins were observed in LNCaP cells treated with RANKL or in LN RANKL cells as evaluated by western blot analysis. Lamin A/C was used as internal control. (D) Activities of human RANKL, (E) c-Met, and (F) AR promoter-luciferase reporter constructs were compared between LN RANKL and LN Neo cells. Site-directed mutagenesis was used to remove the E-box, the cis -element required for c-Myc/Max interaction. Complementary experiments confirmed the results by assaying promoter reporter activities under 10058-F4 (40 μM) exposure, known to inhibit c-Myc function by interference with its dimerization with Max, and by shRNA knockdown of the c-Myc, Max, or AP4 to suppress c-Myc/Max or AP4 binding to its E-box cis -element (* P
    Figure Legend Snippet: RANKL induces expression of RANKL and c-Met but suppresses AR expression/activation through c-Myc/Max and AP4 transcription factors in the LNCaP cell background. (A) RANKL overexpression upregulates endogenous RANKL and c-Met expression and c-Met phosphorylation but suppresses AR expression. (B) Exogenous RANKL treatment or intrinsic RANKL expression induces endogenous RANKL expression, c-Met expression, and c-Met phosphorylation. Inductions are partly sensitive to OPG or anti-RANKL antibody (denosumab) treatments. (C) RANKL–RANK signaling increases the level of c-Myc and Max expression. Increased c-Myc and Max nuclear proteins were observed in LNCaP cells treated with RANKL or in LN RANKL cells as evaluated by western blot analysis. Lamin A/C was used as internal control. (D) Activities of human RANKL, (E) c-Met, and (F) AR promoter-luciferase reporter constructs were compared between LN RANKL and LN Neo cells. Site-directed mutagenesis was used to remove the E-box, the cis -element required for c-Myc/Max interaction. Complementary experiments confirmed the results by assaying promoter reporter activities under 10058-F4 (40 μM) exposure, known to inhibit c-Myc function by interference with its dimerization with Max, and by shRNA knockdown of the c-Myc, Max, or AP4 to suppress c-Myc/Max or AP4 binding to its E-box cis -element (* P

    Techniques Used: Expressing, Activation Assay, Over Expression, Western Blot, Luciferase, Construct, Mutagenesis, shRNA, Binding Assay

    RANKL promotes stem cell and neuroendocrine properties of PCa cells. LN RANKL cells have elevated gene expression of (A) stem cell markers and (B) neuroendocrine markers confirmed by qRT-PCR. (C) Upregulation of stem cell markers by RANKL expression can be attenuated by OPG and denosumab treatments as analyzed by qRT-PCR, western blot, and flow cytometry. (D) OPG and denosumab also downregulate the expression of neuroendocrine markers in LN RANKL cells, confirmed by both qRT-PCR and western blot analyses. (B, C and D) Statistical significance of differences between the treatment groups and the control group (LN RANKL ) were calculated based on a global comparison for the set of genes. * P
    Figure Legend Snippet: RANKL promotes stem cell and neuroendocrine properties of PCa cells. LN RANKL cells have elevated gene expression of (A) stem cell markers and (B) neuroendocrine markers confirmed by qRT-PCR. (C) Upregulation of stem cell markers by RANKL expression can be attenuated by OPG and denosumab treatments as analyzed by qRT-PCR, western blot, and flow cytometry. (D) OPG and denosumab also downregulate the expression of neuroendocrine markers in LN RANKL cells, confirmed by both qRT-PCR and western blot analyses. (B, C and D) Statistical significance of differences between the treatment groups and the control group (LN RANKL ) were calculated based on a global comparison for the set of genes. * P

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot, Flow Cytometry

    RANKL overexpression in PCa cells confers increased lethal and osteolytic bone metastasis in nude mice. (A) RANKL, RANK, and OPG expression was assessed in human PCa ARCaP E and ARCaP M EMT cell model and human LN Neo and LN RANKL cells by qRT-PCR (* P
    Figure Legend Snippet: RANKL overexpression in PCa cells confers increased lethal and osteolytic bone metastasis in nude mice. (A) RANKL, RANK, and OPG expression was assessed in human PCa ARCaP E and ARCaP M EMT cell model and human LN Neo and LN RANKL cells by qRT-PCR (* P

    Techniques Used: Over Expression, Mouse Assay, Expressing, Quantitative RT-PCR

    6) Product Images from "Substance P Promotes the Proliferation, but Inhibits Differentiation and Mineralization of Osteoblasts from Rats with Spinal Cord Injury via RANKL/OPG System"

    Article Title: Substance P Promotes the Proliferation, but Inhibits Differentiation and Mineralization of Osteoblasts from Rats with Spinal Cord Injury via RANKL/OPG System

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0165063

    The expression of Osteoblastic markers in the bone microenvironment of proximal tibiae and the effect of 10 −8 M SP on levels of RANKL/OPG as well as on osteoblastic markers in vitro. (A) RANKL, OPG, Col1α1, ALP, and OC mRNA expression in the proximal tibiae of SCI and SHAM rats. (B) RANKL, OPG, Col1α1, ALP and OC mRNA expression in BMSC-OB treated with 10 −8 M SP. The values of expression levels were pooled from 10 rats per group and expressed as mean ± SD. (C) Western blot analysis for RANKL, OPG and NK1R proteins expression in the proximal tibiae and the expression levels were quantified by densitometry. (D) Western blot analysis for RANKL, OPG proteins expression on BMSC-OB treated with 10 −8 M SP and the expression levels were quantified by densitometry. Equal loading of protein and transfer were assessed with β-actin expression. The values of expression levels were pooled from 10 rats per group and expressed as mean ± SD. All blots were visualized by chemiluminescence, * p
    Figure Legend Snippet: The expression of Osteoblastic markers in the bone microenvironment of proximal tibiae and the effect of 10 −8 M SP on levels of RANKL/OPG as well as on osteoblastic markers in vitro. (A) RANKL, OPG, Col1α1, ALP, and OC mRNA expression in the proximal tibiae of SCI and SHAM rats. (B) RANKL, OPG, Col1α1, ALP and OC mRNA expression in BMSC-OB treated with 10 −8 M SP. The values of expression levels were pooled from 10 rats per group and expressed as mean ± SD. (C) Western blot analysis for RANKL, OPG and NK1R proteins expression in the proximal tibiae and the expression levels were quantified by densitometry. (D) Western blot analysis for RANKL, OPG proteins expression on BMSC-OB treated with 10 −8 M SP and the expression levels were quantified by densitometry. Equal loading of protein and transfer were assessed with β-actin expression. The values of expression levels were pooled from 10 rats per group and expressed as mean ± SD. All blots were visualized by chemiluminescence, * p

    Techniques Used: Expressing, In Vitro, ALP Assay, Western Blot

    7) Product Images from "Ovariectomy increases vascular calcification via the OPG/RANKL cytokine signalling pathway"

    Article Title: Ovariectomy increases vascular calcification via the OPG/RANKL cytokine signalling pathway

    Journal:

    doi: 10.1111/j.1365-2362.2008.01930.x

    Bar graph quantification and representative Western blot images for osteoprotegerin (OPG), receptor activator of nuclear factor-κB (RANKL) and GADPH (Arbitrary Units ± SEM); * P
    Figure Legend Snippet: Bar graph quantification and representative Western blot images for osteoprotegerin (OPG), receptor activator of nuclear factor-κB (RANKL) and GADPH (Arbitrary Units ± SEM); * P

    Techniques Used: Western Blot

    8) Product Images from "Vitamin D3 Modulates Impaired Crosstalk Between RANK and Glucocorticoid Receptor Signaling in Bone Marrow Cells After Chronic Prednisolone Administration"

    Article Title: Vitamin D3 Modulates Impaired Crosstalk Between RANK and Glucocorticoid Receptor Signaling in Bone Marrow Cells After Chronic Prednisolone Administration

    Journal: Frontiers in Endocrinology

    doi: 10.3389/fendo.2018.00303

    Effects of prednisolone and vitamin D 3 administration on the RANKL/RANK/osteoprotegerin (OPG) signaling pathway: 1—control; 2—prednisolone administration (5 mg/kg of b.w.); and 3—prednisolone and vitamin D 3 (1,000 IU/kg of b.w.) administration. Immunoblotting analysis of RANKL, RANK, and OPG in rat bone marrow (BM): representative immunoblots are shown (A) and quantified using β-actin as a loading control for total BM lysates. The bar graphs of RANKL (B) , RANK (C) , and OPG (E) are presented as means ± SEM ( n = 6/group). Quantitative polymerase chain reaction of Rankl in rat BM (B) : data were normalized to Gapdh and pooled from two independent experiments ( n = 6 rats/group). RANK-positive BM cells (D) : representative histograms (count—the number of events; FL1 LOG—fluorescence intensity) and quantification of RANK - positive cells documented by flow cytometry analysis. All data are shown as means ± SEM; * p
    Figure Legend Snippet: Effects of prednisolone and vitamin D 3 administration on the RANKL/RANK/osteoprotegerin (OPG) signaling pathway: 1—control; 2—prednisolone administration (5 mg/kg of b.w.); and 3—prednisolone and vitamin D 3 (1,000 IU/kg of b.w.) administration. Immunoblotting analysis of RANKL, RANK, and OPG in rat bone marrow (BM): representative immunoblots are shown (A) and quantified using β-actin as a loading control for total BM lysates. The bar graphs of RANKL (B) , RANK (C) , and OPG (E) are presented as means ± SEM ( n = 6/group). Quantitative polymerase chain reaction of Rankl in rat BM (B) : data were normalized to Gapdh and pooled from two independent experiments ( n = 6 rats/group). RANK-positive BM cells (D) : representative histograms (count—the number of events; FL1 LOG—fluorescence intensity) and quantification of RANK - positive cells documented by flow cytometry analysis. All data are shown as means ± SEM; * p

    Techniques Used: Western Blot, Real-time Polymerase Chain Reaction, Fluorescence, Flow Cytometry, Cytometry

    9) Product Images from "Effect of serum from postmenopausal women with osteoporosis exhibiting the Kidney-Yang deficiency pattern on bone formation in an hFOB 1.19 human osteoblastic cell line"

    Article Title: Effect of serum from postmenopausal women with osteoporosis exhibiting the Kidney-Yang deficiency pattern on bone formation in an hFOB 1.19 human osteoblastic cell line

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2015.2616

    Effect of KYD pattern-serum on the expression of osteocalcin, OPG and RANKL. Total protein was isolated from the hFOB 1.19 cells treated with the KYD pattern-serum for 72 h, and (A) western blotting was performed in order to determine the protein levels of (B) osteocalcin, (C) OPG and (D) RANKL, which were normalized to the levels of β-actin. Total RNA was isolated and the quantitative polymerase chain reaction was performed to determine the mRNA expression of (E) osteocalcin, (F) OPG and (G) RANKL, which was normalized to that of GAPDH. Data are presented as the mean ± standard deviation (error bars) from at least three independent experiments. a P
    Figure Legend Snippet: Effect of KYD pattern-serum on the expression of osteocalcin, OPG and RANKL. Total protein was isolated from the hFOB 1.19 cells treated with the KYD pattern-serum for 72 h, and (A) western blotting was performed in order to determine the protein levels of (B) osteocalcin, (C) OPG and (D) RANKL, which were normalized to the levels of β-actin. Total RNA was isolated and the quantitative polymerase chain reaction was performed to determine the mRNA expression of (E) osteocalcin, (F) OPG and (G) RANKL, which was normalized to that of GAPDH. Data are presented as the mean ± standard deviation (error bars) from at least three independent experiments. a P

    Techniques Used: Expressing, Isolation, Western Blot, Real-time Polymerase Chain Reaction, Standard Deviation

    10) Product Images from "Therapeutic effects of radix dipsaci, pyrola herb, and cynomorium songaricum on bone metabolism of ovariectomized rats"

    Article Title: Therapeutic effects of radix dipsaci, pyrola herb, and cynomorium songaricum on bone metabolism of ovariectomized rats

    Journal: BMC Complementary and Alternative Medicine

    doi: 10.1186/1472-6882-12-67

    Effects of RDD, PHD, and CSD on protein expression of OPG and RANKL. A ) Immunohistochemical stain of OPG; B ) Immunohistochemical stain of RANKL; C ) Ratio of RANKL/OPG protein expression, RANKL and OPG protein expression data were obtained from panels A and B of this figure, see details in the Methods section of the text, (a, p
    Figure Legend Snippet: Effects of RDD, PHD, and CSD on protein expression of OPG and RANKL. A ) Immunohistochemical stain of OPG; B ) Immunohistochemical stain of RANKL; C ) Ratio of RANKL/OPG protein expression, RANKL and OPG protein expression data were obtained from panels A and B of this figure, see details in the Methods section of the text, (a, p

    Techniques Used: Expressing, Immunohistochemistry, Staining

    Effects of RDD, PHD, and CSD on expression of OPG and RANKL mRNA. A ) In situ hybridization of OPG in OB and MSC; B ) In situ hybridization of RANKL in OB and bMSC; C ) Ratio of RANKL/OPG mRNA expression, RANKL and OPG mRNA expression data were obtained from panels A and C of this figure, see details in the Methods section of the text, (a, p
    Figure Legend Snippet: Effects of RDD, PHD, and CSD on expression of OPG and RANKL mRNA. A ) In situ hybridization of OPG in OB and MSC; B ) In situ hybridization of RANKL in OB and bMSC; C ) Ratio of RANKL/OPG mRNA expression, RANKL and OPG mRNA expression data were obtained from panels A and C of this figure, see details in the Methods section of the text, (a, p

    Techniques Used: Expressing, In Situ Hybridization

    11) Product Images from "Effects of Processed Polygonum multiflorum with KIOM Patent on Bone Remodeling-Related Protein Expression in Human Osteoblast-Like SaOS-2 Cells"

    Article Title: Effects of Processed Polygonum multiflorum with KIOM Patent on Bone Remodeling-Related Protein Expression in Human Osteoblast-Like SaOS-2 Cells

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2020/4168535

    Effects of samples on osteoclast differentiation mediators, RANKL, and OPG in SaOS-2 cells. (a). Effects of samples on RANKL protein levels in SaOS-2 osteosarcoma and expression of RANKL, osteoclast differentiation factor, in SaOS-2 osteosarcoma cells. (b). Effects of samples on OPG protein levels in SaOS-2 osteosarcoma. Expression of OPG, RANKL inhibitor, in SaOS-2 osteosarcoma cells † p
    Figure Legend Snippet: Effects of samples on osteoclast differentiation mediators, RANKL, and OPG in SaOS-2 cells. (a). Effects of samples on RANKL protein levels in SaOS-2 osteosarcoma and expression of RANKL, osteoclast differentiation factor, in SaOS-2 osteosarcoma cells. (b). Effects of samples on OPG protein levels in SaOS-2 osteosarcoma. Expression of OPG, RANKL inhibitor, in SaOS-2 osteosarcoma cells † p

    Techniques Used: Expressing

    12) Product Images from "An Oligodeoxynucleotide with Promising Modulation Activity for the Proliferation and Activation of Osteoblast"

    Article Title: An Oligodeoxynucleotide with Promising Modulation Activity for the Proliferation and Activation of Osteoblast

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms12042543

    Western blotting analysis of expression level of Sp7, runx-2, collagen-I, OPG and RANKL in ODN MT01 treatment group and control group at predetermined time.
    Figure Legend Snippet: Western blotting analysis of expression level of Sp7, runx-2, collagen-I, OPG and RANKL in ODN MT01 treatment group and control group at predetermined time.

    Techniques Used: Western Blot, Expressing

    13) Product Images from "Expression of Osteoprotegrin and Osteoclast Level in Chronic Apical Periodontitis Induced with East Java Propolis Extract"

    Article Title: Expression of Osteoprotegrin and Osteoclast Level in Chronic Apical Periodontitis Induced with East Java Propolis Extract

    Journal: Iranian Endodontic Journal

    doi: 10.22037/iej.v13i1.18781

    OPG Expression in periapical Wistar rats. A) The oval section represents a periapical area at 400× magnification in Control group; B) E. faecalis group; C) Propolis group; D, E, F) Under 1000× magnification then was conducted to confirm the oval area
    Figure Legend Snippet: OPG Expression in periapical Wistar rats. A) The oval section represents a periapical area at 400× magnification in Control group; B) E. faecalis group; C) Propolis group; D, E, F) Under 1000× magnification then was conducted to confirm the oval area

    Techniques Used: Expressing

    14) Product Images from "Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia"

    Article Title: Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia

    Journal: Theranostics

    doi: 10.7150/thno.27372

    BMMSCs of HPP patients exhibit diminished osteogenic differentiation but preferential adipogenic differentiation. (A) Double immunostaining showed that CD146+ (red) human BMMSCs co-expressed TNSALP (green). Scale bars, 50 μm. (B) TNSALP expression was analyzed by flow cytometry analysis (FCM). (C) The ALP activity and TNSALP expression in normal and HPP BMMSCs were examined using the ALP activity assay and western blotting. (D) Alizarin red staining and quantification of mineralized nodules were performed at day 28 after osteogenic induction (OS). Runx2 and OCN expression levels were examined at day 7 after induction by western blotting. (E) HE/Masson's trichrome staining and quantitative analysis showed formation of bone (B), bone marrow (BM), and collagen fiber (CF) around HA/TCP (HA) carrier when BMMSCs were implanted into nude mice. Scale bars, 200 μm. (F) Oil Red O staining and quantification of fat depots were performed at day 14 after adipogenic induction (AD). PPAR-γ expression was examined at day 7 after induction by western blotting. Scale bars, 100 μm. (G) OPG and RANKL expression levels in BMMSCs were examined by western blotting. Normal control n = 5, HPP n = 2. The data are presented as mean ± s.d. for triplicate samples from a representative experiment. * P
    Figure Legend Snippet: BMMSCs of HPP patients exhibit diminished osteogenic differentiation but preferential adipogenic differentiation. (A) Double immunostaining showed that CD146+ (red) human BMMSCs co-expressed TNSALP (green). Scale bars, 50 μm. (B) TNSALP expression was analyzed by flow cytometry analysis (FCM). (C) The ALP activity and TNSALP expression in normal and HPP BMMSCs were examined using the ALP activity assay and western blotting. (D) Alizarin red staining and quantification of mineralized nodules were performed at day 28 after osteogenic induction (OS). Runx2 and OCN expression levels were examined at day 7 after induction by western blotting. (E) HE/Masson's trichrome staining and quantitative analysis showed formation of bone (B), bone marrow (BM), and collagen fiber (CF) around HA/TCP (HA) carrier when BMMSCs were implanted into nude mice. Scale bars, 200 μm. (F) Oil Red O staining and quantification of fat depots were performed at day 14 after adipogenic induction (AD). PPAR-γ expression was examined at day 7 after induction by western blotting. Scale bars, 100 μm. (G) OPG and RANKL expression levels in BMMSCs were examined by western blotting. Normal control n = 5, HPP n = 2. The data are presented as mean ± s.d. for triplicate samples from a representative experiment. * P

    Techniques Used: Double Immunostaining, Expressing, Flow Cytometry, Cytometry, ALP Assay, Activity Assay, Western Blot, Staining, Mouse Assay

    15) Product Images from "High-Dose Diosgenin Reduces Bone Loss in Ovariectomized Rats via Attenuation of the RANKL/OPG Ratio"

    Article Title: High-Dose Diosgenin Reduces Bone Loss in Ovariectomized Rats via Attenuation of the RANKL/OPG Ratio

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms150917130

    Effects of 12 weeks treatment on expression of OPG and RANKL mRNA in tibiae of rats. Expression level of OPG and RANKL mRNA was estimated by using in situ hybridization. ( A ) OPG mRNA expression; ( B ) RANKL mRNA expression, and ( C ) mRNA ratio of RANKL/OPG are shown. In panel ( C ), a, p
    Figure Legend Snippet: Effects of 12 weeks treatment on expression of OPG and RANKL mRNA in tibiae of rats. Expression level of OPG and RANKL mRNA was estimated by using in situ hybridization. ( A ) OPG mRNA expression; ( B ) RANKL mRNA expression, and ( C ) mRNA ratio of RANKL/OPG are shown. In panel ( C ), a, p

    Techniques Used: Expressing, In Situ Hybridization

    Effects of 12 weeks treatment on expression of OPG and RANKL protein in tibiae of rats. Expression level of OPG and RANKL protein was estimated by immunohistochemical analysis. ( A ) OPG expression; ( B ) RANKL expression; and ( C ) Ratio of RANKL/OPG are shown. In panel ( C ), a, p
    Figure Legend Snippet: Effects of 12 weeks treatment on expression of OPG and RANKL protein in tibiae of rats. Expression level of OPG and RANKL protein was estimated by immunohistochemical analysis. ( A ) OPG expression; ( B ) RANKL expression; and ( C ) Ratio of RANKL/OPG are shown. In panel ( C ), a, p

    Techniques Used: Expressing, Immunohistochemistry

    16) Product Images from "Stiffness of Nanoparticulate Mineralized Collagen Scaffolds Triggers Osteogenesis via Mechanotransduction and Canonical Wnt Signaling"

    Article Title: Stiffness of Nanoparticulate Mineralized Collagen Scaffolds Triggers Osteogenesis via Mechanotransduction and Canonical Wnt Signaling

    Journal: bioRxiv

    doi: 10.1101/2020.03.09.982231

    Stiffness increases expression of osteogenic genes and activation of the canonical BMP receptor and Wnt signaling pathways QPCR of primary hMSCs cultured on NX-MC or MC for 3 or 7 days in osteogenic differentiation medium for ( A ) ALP, ( B ) COL1A1, ( C ) RUNX2, ( J ) BMP2, ( K ) BMP4, and ( L ) BMP7 (n=3). Merged representative immunofluorescent images of phosphorylated Smad1/5 (p-Smad1/5) and Dapi costaining ( E ) and quantification ( F ) of p-Smad1/5 staining of primary hMSCs cultured on NX-MC or MC for 3 or 7 days. Western blot of primary hMSCs cultured on NX-MC or MC for 0, 3, 7, or 14 days in osteogenic differentiation medium for ( D ) Runx2, OPG, and β-actin or ( G ) p-Smad1/5, total Smad5, p-ERK1/2, and total ERK1/2. Densitometric quantification of western blot analyses demonstrating relative protein amounts of p-Smad1/5 to total Smad5 ( H ) and p-ERK1/2 to total ERK1/2 ( I ). Bars represent means, errors bars represent SE. Significant posthoc comparisons following ANOVA indicated with p values.
    Figure Legend Snippet: Stiffness increases expression of osteogenic genes and activation of the canonical BMP receptor and Wnt signaling pathways QPCR of primary hMSCs cultured on NX-MC or MC for 3 or 7 days in osteogenic differentiation medium for ( A ) ALP, ( B ) COL1A1, ( C ) RUNX2, ( J ) BMP2, ( K ) BMP4, and ( L ) BMP7 (n=3). Merged representative immunofluorescent images of phosphorylated Smad1/5 (p-Smad1/5) and Dapi costaining ( E ) and quantification ( F ) of p-Smad1/5 staining of primary hMSCs cultured on NX-MC or MC for 3 or 7 days. Western blot of primary hMSCs cultured on NX-MC or MC for 0, 3, 7, or 14 days in osteogenic differentiation medium for ( D ) Runx2, OPG, and β-actin or ( G ) p-Smad1/5, total Smad5, p-ERK1/2, and total ERK1/2. Densitometric quantification of western blot analyses demonstrating relative protein amounts of p-Smad1/5 to total Smad5 ( H ) and p-ERK1/2 to total ERK1/2 ( I ). Bars represent means, errors bars represent SE. Significant posthoc comparisons following ANOVA indicated with p values.

    Techniques Used: Expressing, Activation Assay, Real-time Polymerase Chain Reaction, Cell Culture, Staining, Western Blot

    Inhibition of Rho GTPase or F-actin polymerization reduces active β-catenin with a compensatory increase in p-Smad1/5 and BMP2 expression Western blot of primary hMSCs cultured on NX-MC or MC for 0 or 3 days in osteogenic differentiation medium untreated, treated with 0.5 μM of LA, or treated with 3 μg/mL of C3 for ( A ) Runx2, OPG, non-p-β-catenin, and GAPDH or ( D ) p-Smad1/5 and Smad5. Densitometric quantification of western blot analyses demonstrating relative protein amounts of ( B ) Runx2 to GAPDH, ( C ) non-p-β-catenin to GAPDH, or ( E ) p-Smad1/5 to Smad5. QPCR of primary hMSCs cultured on NX-MC or MC for 0 or 3 days in osteogenic differentiation medium untreated, treated with 0.5 μM of LA, or treated with 3 μg/mL of C3 for ( F ) BMP2, ( G ) BMP4, and ( H ) BMP7. Bars represent means, errors bars represent SE. Significant posthoc comparisons following ANOVA indicated with p values.
    Figure Legend Snippet: Inhibition of Rho GTPase or F-actin polymerization reduces active β-catenin with a compensatory increase in p-Smad1/5 and BMP2 expression Western blot of primary hMSCs cultured on NX-MC or MC for 0 or 3 days in osteogenic differentiation medium untreated, treated with 0.5 μM of LA, or treated with 3 μg/mL of C3 for ( A ) Runx2, OPG, non-p-β-catenin, and GAPDH or ( D ) p-Smad1/5 and Smad5. Densitometric quantification of western blot analyses demonstrating relative protein amounts of ( B ) Runx2 to GAPDH, ( C ) non-p-β-catenin to GAPDH, or ( E ) p-Smad1/5 to Smad5. QPCR of primary hMSCs cultured on NX-MC or MC for 0 or 3 days in osteogenic differentiation medium untreated, treated with 0.5 μM of LA, or treated with 3 μg/mL of C3 for ( F ) BMP2, ( G ) BMP4, and ( H ) BMP7. Bars represent means, errors bars represent SE. Significant posthoc comparisons following ANOVA indicated with p values.

    Techniques Used: Inhibition, Expressing, Western Blot, Cell Culture, Real-time Polymerase Chain Reaction

    17) Product Images from "Therapeutic Effects of Cortex acanthopanacis Aqueous Extract on Bone Metabolism of Ovariectomized Rats"

    Article Title: Therapeutic Effects of Cortex acanthopanacis Aqueous Extract on Bone Metabolism of Ovariectomized Rats

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2012/492627

    Effects of 12-week treatment with Cortex acanthopanacis extract (CAE) or Folium Epimedii extract (FEE) on protein expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL) for the NC (normal control rats), Sham (rats with Sham operation), OVX (ovariectomized rats), E2 (rats treated with 17 β -estradiol), FEE (rats treated with FEE), and CAE (rats treated with CAE) groups. Results are expressed as mean ± SD, for n = 12. a P
    Figure Legend Snippet: Effects of 12-week treatment with Cortex acanthopanacis extract (CAE) or Folium Epimedii extract (FEE) on protein expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL) for the NC (normal control rats), Sham (rats with Sham operation), OVX (ovariectomized rats), E2 (rats treated with 17 β -estradiol), FEE (rats treated with FEE), and CAE (rats treated with CAE) groups. Results are expressed as mean ± SD, for n = 12. a P

    Techniques Used: Expressing

    Effects of 12-week treatment with Cortex acanthopanacis extract (CAE) or Folium Epimedii extract (FEE) on mRNA expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL) for the NC (normal control rats), Sham (rats with Sham operation), OVX (ovariectomized rats), E2 (rats treated with 17 β -estradiol), FEE (rats treated with FEE), and CAE (rats treated with CAE) groups. Results are expressed as mean ± SD, for n = 12. a P
    Figure Legend Snippet: Effects of 12-week treatment with Cortex acanthopanacis extract (CAE) or Folium Epimedii extract (FEE) on mRNA expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL) for the NC (normal control rats), Sham (rats with Sham operation), OVX (ovariectomized rats), E2 (rats treated with 17 β -estradiol), FEE (rats treated with FEE), and CAE (rats treated with CAE) groups. Results are expressed as mean ± SD, for n = 12. a P

    Techniques Used: Expressing

    Effects of 12-week treatment with Cortex acanthopanacis extract (CAE) or Folium Epimedii extract (FEE) on ratio between receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG) expression for the NC (normal control rats), Sham (rats with Sham operation), OVX (ovariectomized rats), E2 (rats treated with 17 β -estradiol), FEE (rats treated with FEE), and CAE (rats treated with CAE) groups. Results are expressed as mean ± SD, for n = 12. a P
    Figure Legend Snippet: Effects of 12-week treatment with Cortex acanthopanacis extract (CAE) or Folium Epimedii extract (FEE) on ratio between receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG) expression for the NC (normal control rats), Sham (rats with Sham operation), OVX (ovariectomized rats), E2 (rats treated with 17 β -estradiol), FEE (rats treated with FEE), and CAE (rats treated with CAE) groups. Results are expressed as mean ± SD, for n = 12. a P

    Techniques Used: Expressing

    18) Product Images from "Spinal cord injury causes bone loss through peroxisome proliferator-activated receptor-γ and Wnt signalling"

    Article Title: Spinal cord injury causes bone loss through peroxisome proliferator-activated receptor-γ and Wnt signalling

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/j.1582-4934.2012.01624.x

    In vitro effects of GW9662 and troglitazone (TGZ) on RANKL mRNA, OPG mRNA, Wnt signalling and protein secretion in mesenchymal stem cells (MSC) from spinal cord injury (SCI) and SHAM rats. (A) TGZ up-regulated RANKL/OPG ratio, whereas GW9662 down-regulated RANKL/OPG ratio in MSCs from SCI rats as compared with that of SHAM rats. And, TGZ down-regulated Wnt1 in MSCs from SCI rats as compared with that of SHAM rats. (B–E) The adiponectin levels in the medium were significantly higher in MSCs from SCI rats than SHAM rats, whereas the ALP and OPG levels were significantly lower in MSCs from SCI rats than SHAM rats. There was no significant difference of leptin levels in the medium between MSCs from SCI rats and SHAM rats. The ALP abd OPG levels in the medium were decreased in MSCs treated with TGZ, and increased in MSCs treated with GW9662. The values of medium levels were pooled from 10 samples per group and expressed as averages ± SE. * P
    Figure Legend Snippet: In vitro effects of GW9662 and troglitazone (TGZ) on RANKL mRNA, OPG mRNA, Wnt signalling and protein secretion in mesenchymal stem cells (MSC) from spinal cord injury (SCI) and SHAM rats. (A) TGZ up-regulated RANKL/OPG ratio, whereas GW9662 down-regulated RANKL/OPG ratio in MSCs from SCI rats as compared with that of SHAM rats. And, TGZ down-regulated Wnt1 in MSCs from SCI rats as compared with that of SHAM rats. (B–E) The adiponectin levels in the medium were significantly higher in MSCs from SCI rats than SHAM rats, whereas the ALP and OPG levels were significantly lower in MSCs from SCI rats than SHAM rats. There was no significant difference of leptin levels in the medium between MSCs from SCI rats and SHAM rats. The ALP abd OPG levels in the medium were decreased in MSCs treated with TGZ, and increased in MSCs treated with GW9662. The values of medium levels were pooled from 10 samples per group and expressed as averages ± SE. * P

    Techniques Used: In Vitro, ALP Assay

    peroxisome proliferator-activated receptor-γ (PPARγ) expression is increased and Wnt signalling is diminished in the skeleton of spinal cord injury (SCI) rats compared with that of SHAM rats. (A) Expression levels of Wnt1, Wnt5a, Lef1, Lrp5 and ctnnb1 mRNA in tibiae of SCI and SHAM rats. (B) Expression levels of PPARγ, αP2, LPL, OPG and RANKL mRNA in humeri and tibiae of SCI and SHAM rats. cDNA synthesis was performed from RNA which was extracted from the respective tibiae. The cDNAs were amplified using real-time PCR. The values of expression levels were pooled from 10 rats per group and expressed as averages ± SE. * P
    Figure Legend Snippet: peroxisome proliferator-activated receptor-γ (PPARγ) expression is increased and Wnt signalling is diminished in the skeleton of spinal cord injury (SCI) rats compared with that of SHAM rats. (A) Expression levels of Wnt1, Wnt5a, Lef1, Lrp5 and ctnnb1 mRNA in tibiae of SCI and SHAM rats. (B) Expression levels of PPARγ, αP2, LPL, OPG and RANKL mRNA in humeri and tibiae of SCI and SHAM rats. cDNA synthesis was performed from RNA which was extracted from the respective tibiae. The cDNAs were amplified using real-time PCR. The values of expression levels were pooled from 10 rats per group and expressed as averages ± SE. * P

    Techniques Used: Expressing, Amplification, Real-time Polymerase Chain Reaction

    In vitro effects of GW9662 and troglitazone (TGZ) on RANKL and OPG protein expression and in vitro effects of BIO and Dickkopf 1 (DKK1) on peroxisome proliferator-activated receptor-γ (PPARγ) protein expression in mesenchymal stem cells (MSC) from spinal cord injury (SCI) and SHAM rats. (A) Representative PPARγ protein expression in MSCs from SCI and SHAM rats treated with BIO and DKK1; representative OPG and RANKL protein expression in MSCs from SCI and SHAM rats treated with GW9662 and TGZ. (B) There were no significant effects of BIO and DKK1 on PPARγ protein expression in MSCs from SCI and SHAM rats. (C) TGZ up-regulated RANKL/OPG ratio, whereas GW9662 down-regulated RANKL/OPG ratio in MSCs from SCI rats as compared with SHAM rats.
    Figure Legend Snippet: In vitro effects of GW9662 and troglitazone (TGZ) on RANKL and OPG protein expression and in vitro effects of BIO and Dickkopf 1 (DKK1) on peroxisome proliferator-activated receptor-γ (PPARγ) protein expression in mesenchymal stem cells (MSC) from spinal cord injury (SCI) and SHAM rats. (A) Representative PPARγ protein expression in MSCs from SCI and SHAM rats treated with BIO and DKK1; representative OPG and RANKL protein expression in MSCs from SCI and SHAM rats treated with GW9662 and TGZ. (B) There were no significant effects of BIO and DKK1 on PPARγ protein expression in MSCs from SCI and SHAM rats. (C) TGZ up-regulated RANKL/OPG ratio, whereas GW9662 down-regulated RANKL/OPG ratio in MSCs from SCI rats as compared with SHAM rats.

    Techniques Used: In Vitro, Expressing

    19) Product Images from "Lanthanum carbonate prevents accelerated medial calcification in uremic rats: role of osteoclast-like activity"

    Article Title: Lanthanum carbonate prevents accelerated medial calcification in uremic rats: role of osteoclast-like activity

    Journal: Journal of Translational Medicine

    doi: 10.1186/1479-5876-11-308

    Aorta for evidence of VSMC phenotype change by performing immunochemistry. Expression of CathepsinK (A-C) , OPG (D-F) , RANKL (G-I) , TRAP (J-L) , Runx2 (M-O) , and Osteocalcin (P-R) were detected in the aortic tunica media of normal, CRF and 2%La treatment rats. Arrows indicate positively stained action. All sections were of the thoracic aorta region.
    Figure Legend Snippet: Aorta for evidence of VSMC phenotype change by performing immunochemistry. Expression of CathepsinK (A-C) , OPG (D-F) , RANKL (G-I) , TRAP (J-L) , Runx2 (M-O) , and Osteocalcin (P-R) were detected in the aortic tunica media of normal, CRF and 2%La treatment rats. Arrows indicate positively stained action. All sections were of the thoracic aorta region.

    Techniques Used: Expressing, Staining

    20) Product Images from "Combined effect of recombinant human bone morphogenetic protein-2 and low level laser irradiation on bisphosphonate-treated osteoblasts"

    Article Title: Combined effect of recombinant human bone morphogenetic protein-2 and low level laser irradiation on bisphosphonate-treated osteoblasts

    Journal: Journal of the Korean Association of Oral and Maxillofacial Surgeons

    doi: 10.5125/jkaoms.2018.44.6.259

    Effects of alendronate, recombinant human bone morphogenetic protein-2 (rhBMP-2), and low-level laser therapy (LLLT) on receptor activator of nuclear factor kappa-B (RANKL), osteoprotegerin (OPG), and macrophage colony-stimulating factor (M-CSF) expression as determined by real-time polymerase chain reaction. A. RANKL expression. B. OPG expression. C. M-CSF expression. Treatment with rhBMP-2 resulted in greater effects on OPG and M-CSF expression, while treatment with LLLT had an effect on expression of RANKL. However, the differences between the effects of rhBMP-2 and LLLT were not statistically significant. * P
    Figure Legend Snippet: Effects of alendronate, recombinant human bone morphogenetic protein-2 (rhBMP-2), and low-level laser therapy (LLLT) on receptor activator of nuclear factor kappa-B (RANKL), osteoprotegerin (OPG), and macrophage colony-stimulating factor (M-CSF) expression as determined by real-time polymerase chain reaction. A. RANKL expression. B. OPG expression. C. M-CSF expression. Treatment with rhBMP-2 resulted in greater effects on OPG and M-CSF expression, while treatment with LLLT had an effect on expression of RANKL. However, the differences between the effects of rhBMP-2 and LLLT were not statistically significant. * P

    Techniques Used: Recombinant, Expressing, Real-time Polymerase Chain Reaction

    21) Product Images from "Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia"

    Article Title: Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia

    Journal: Theranostics

    doi: 10.7150/thno.27372

    BMMSCs of HPP patients exhibit diminished osteogenic differentiation but preferential adipogenic differentiation. (A) Double immunostaining showed that CD146+ (red) human BMMSCs co-expressed TNSALP (green). Scale bars, 50 μm. (B) TNSALP expression was analyzed by flow cytometry analysis (FCM). (C) The ALP activity and TNSALP expression in normal and HPP BMMSCs were examined using the ALP activity assay and western blotting. (D) Alizarin red staining and quantification of mineralized nodules were performed at day 28 after osteogenic induction (OS). Runx2 and OCN expression levels were examined at day 7 after induction by western blotting. (E) HE/Masson's trichrome staining and quantitative analysis showed formation of bone (B), bone marrow (BM), and collagen fiber (CF) around HA/TCP (HA) carrier when BMMSCs were implanted into nude mice. Scale bars, 200 μm. (F) Oil Red O staining and quantification of fat depots were performed at day 14 after adipogenic induction (AD). PPAR-γ expression was examined at day 7 after induction by western blotting. Scale bars, 100 μm. (G) OPG and RANKL expression levels in BMMSCs were examined by western blotting. Normal control n = 5, HPP n = 2. The data are presented as mean ± s.d. for triplicate samples from a representative experiment. * P
    Figure Legend Snippet: BMMSCs of HPP patients exhibit diminished osteogenic differentiation but preferential adipogenic differentiation. (A) Double immunostaining showed that CD146+ (red) human BMMSCs co-expressed TNSALP (green). Scale bars, 50 μm. (B) TNSALP expression was analyzed by flow cytometry analysis (FCM). (C) The ALP activity and TNSALP expression in normal and HPP BMMSCs were examined using the ALP activity assay and western blotting. (D) Alizarin red staining and quantification of mineralized nodules were performed at day 28 after osteogenic induction (OS). Runx2 and OCN expression levels were examined at day 7 after induction by western blotting. (E) HE/Masson's trichrome staining and quantitative analysis showed formation of bone (B), bone marrow (BM), and collagen fiber (CF) around HA/TCP (HA) carrier when BMMSCs were implanted into nude mice. Scale bars, 200 μm. (F) Oil Red O staining and quantification of fat depots were performed at day 14 after adipogenic induction (AD). PPAR-γ expression was examined at day 7 after induction by western blotting. Scale bars, 100 μm. (G) OPG and RANKL expression levels in BMMSCs were examined by western blotting. Normal control n = 5, HPP n = 2. The data are presented as mean ± s.d. for triplicate samples from a representative experiment. * P

    Techniques Used: Double Immunostaining, Expressing, Flow Cytometry, Cytometry, ALP Assay, Activity Assay, Western Blot, Staining, Mouse Assay

    22) Product Images from "Effects of osteoprotegerin, RANK and RANKL on bone destruction and collapse in avascular necrosis femoral head"

    Article Title: Effects of osteoprotegerin, RANK and RANKL on bone destruction and collapse in avascular necrosis femoral head

    Journal: American Journal of Translational Research

    doi:

    Quantitative RT-PCR detection of OPG, RANKL, RANK/h-PBGD mRNA levels. The box plot shows that the figure in the bottom is close to 0 which tips for twenty-fifth percentile. The line inside of the box plot is average value and its upper edge is seventy-fifth
    Figure Legend Snippet: Quantitative RT-PCR detection of OPG, RANKL, RANK/h-PBGD mRNA levels. The box plot shows that the figure in the bottom is close to 0 which tips for twenty-fifth percentile. The line inside of the box plot is average value and its upper edge is seventy-fifth

    Techniques Used: Quantitative RT-PCR

    The expression level comparison of OPG and RANKL/F-actin in normal tissue and necrotic tissue, the box plot shows no obvious difference between them.
    Figure Legend Snippet: The expression level comparison of OPG and RANKL/F-actin in normal tissue and necrotic tissue, the box plot shows no obvious difference between them.

    Techniques Used: Expressing

    OPG (GGkDa) and RANKL (28 kDa) Protein western blot analysis of 4 samples of osteonecrosis of the femoral head; actin (45 kDa) is the control group; AVN and NL are protein lysate which are taken from necrotic tissue and normal tissue respectively.
    Figure Legend Snippet: OPG (GGkDa) and RANKL (28 kDa) Protein western blot analysis of 4 samples of osteonecrosis of the femoral head; actin (45 kDa) is the control group; AVN and NL are protein lysate which are taken from necrotic tissue and normal tissue respectively.

    Techniques Used: Western Blot

    23) Product Images from "Osteoclast-associated receptor blockade prevents articular cartilage destruction via chondrocyte apoptosis regulation"

    Article Title: Osteoclast-associated receptor blockade prevents articular cartilage destruction via chondrocyte apoptosis regulation

    Journal: Nature Communications

    doi: 10.1038/s41467-020-18208-y

    OSCAR-Fc blocks OA pathogenesis in mice. WT mice subjected to sham or DMM surgery were IA-injected with human IgG as a control or hOSCAR-Fc (2 mg kg −1 ) to block OSCAR in joint tissue. Articular cartilage sections were subjected to safranin-O staining ( a ) and quantitative analysis of OARSI grade ( b ), SBP thickness ( c ), and ratio of hyaline cartilage (HC) to calcified cartilage (CC) ( d ). Scale bar = 50 μm. Error bars represent mean ± S.E.M. of n = 10 mice ( b – d ). e – g IHC analyses of MMP3, MMP13, and ADAMTS5 ( e ), aggrecan and COL2A1 ( f ), and TRAIL and OPG ( g ) in articular cartilage tissue from sham surgery or DMM surgery mice ( n = 10). Scale bar = 50 μm. h , i Apoptotic articular chondrocytes were detected and quantified by TUNEL assay. Scale bar = 25 μm. Error bars represent mean ± S.E.M. of n = 10 mice ( i ). Two-way ANOVA was performed followed by Sidak’s Multiple Comparison’s test, with p values indicated in figure.
    Figure Legend Snippet: OSCAR-Fc blocks OA pathogenesis in mice. WT mice subjected to sham or DMM surgery were IA-injected with human IgG as a control or hOSCAR-Fc (2 mg kg −1 ) to block OSCAR in joint tissue. Articular cartilage sections were subjected to safranin-O staining ( a ) and quantitative analysis of OARSI grade ( b ), SBP thickness ( c ), and ratio of hyaline cartilage (HC) to calcified cartilage (CC) ( d ). Scale bar = 50 μm. Error bars represent mean ± S.E.M. of n = 10 mice ( b – d ). e – g IHC analyses of MMP3, MMP13, and ADAMTS5 ( e ), aggrecan and COL2A1 ( f ), and TRAIL and OPG ( g ) in articular cartilage tissue from sham surgery or DMM surgery mice ( n = 10). Scale bar = 50 μm. h , i Apoptotic articular chondrocytes were detected and quantified by TUNEL assay. Scale bar = 25 μm. Error bars represent mean ± S.E.M. of n = 10 mice ( i ). Two-way ANOVA was performed followed by Sidak’s Multiple Comparison’s test, with p values indicated in figure.

    Techniques Used: Mouse Assay, Injection, Blocking Assay, Staining, Immunohistochemistry, TUNEL Assay

    24) Product Images from "β2-adrenergic signal transduction plays a detrimental role in subchondral bone loss of temporomandibular joint in osteoarthritis"

    Article Title: β2-adrenergic signal transduction plays a detrimental role in subchondral bone loss of temporomandibular joint in osteoarthritis

    Journal: Scientific Reports

    doi: 10.1038/srep12593

    Characterization and multi-lineage differentiation capacity of mesenchymal stem cells (MSCs) isolated from condylar subchondral bone of 4-week control (4C) and 4-week experimental (4E) rats, and comparison of their expression of Adrb1/2/3 and pro-osteoclastic factors. A : Flow cytometry analysis showed that MSCs from control and experimental condylar subchondral bone were similarly positive for MSC-associated markers (CD54 and CD90), and negative for hematopoietic markers (CD45 and CD34). B : Osteogeneic, adipogenic and chondrogenic differentiation of MSCs isolated from condylar subchondral bone. C : Real-time PCR analysis of the mRNA expression of β-adrenergic receptors (β-ARs) in MSCs isolated from condylar subchondral bone of 4-wk control and experimental rats. D : Western blot analysis of the Adrb2 expression in MSCs. E : Real-time PCR analysis of the mRNA expression of pro-osteoclastic factors in MSCs. F : Western blot analysis of the RANKL and OPG expression in MSCs. *P
    Figure Legend Snippet: Characterization and multi-lineage differentiation capacity of mesenchymal stem cells (MSCs) isolated from condylar subchondral bone of 4-week control (4C) and 4-week experimental (4E) rats, and comparison of their expression of Adrb1/2/3 and pro-osteoclastic factors. A : Flow cytometry analysis showed that MSCs from control and experimental condylar subchondral bone were similarly positive for MSC-associated markers (CD54 and CD90), and negative for hematopoietic markers (CD45 and CD34). B : Osteogeneic, adipogenic and chondrogenic differentiation of MSCs isolated from condylar subchondral bone. C : Real-time PCR analysis of the mRNA expression of β-adrenergic receptors (β-ARs) in MSCs isolated from condylar subchondral bone of 4-wk control and experimental rats. D : Western blot analysis of the Adrb2 expression in MSCs. E : Real-time PCR analysis of the mRNA expression of pro-osteoclastic factors in MSCs. F : Western blot analysis of the RANKL and OPG expression in MSCs. *P

    Techniques Used: Isolation, Expressing, Flow Cytometry, Cytometry, Real-time Polymerase Chain Reaction, Western Blot

    Expression of RANKL, OPG and RANKL/OPG ratio by MSCs after norepinephrine (NE) treatment. A and B : Real-time PCR of the expression RANKL, OPG and RANKL/OPG ratio by MSCs after NE stimulation. The MSCs were isolated from condylar subchondral bone of 4-week control (4C) and 4-week experimental (4C) rats, and were treated by NE for 2, 6 and 24 h at 0.1, 1 and 10 μM, respectively ( A ). In addition, MSCs from 4-week experimental rats were stimulated by 1 μM NE for 2 h alone, or pre-treated for 1 h with 10 μM propranolol (PRO), 1 μM ICI 118,551 (ICI), 5 μM H-89 or 5 μM U-0126 (U0126) ( B ). *P
    Figure Legend Snippet: Expression of RANKL, OPG and RANKL/OPG ratio by MSCs after norepinephrine (NE) treatment. A and B : Real-time PCR of the expression RANKL, OPG and RANKL/OPG ratio by MSCs after NE stimulation. The MSCs were isolated from condylar subchondral bone of 4-week control (4C) and 4-week experimental (4C) rats, and were treated by NE for 2, 6 and 24 h at 0.1, 1 and 10 μM, respectively ( A ). In addition, MSCs from 4-week experimental rats were stimulated by 1 μM NE for 2 h alone, or pre-treated for 1 h with 10 μM propranolol (PRO), 1 μM ICI 118,551 (ICI), 5 μM H-89 or 5 μM U-0126 (U0126) ( B ). *P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Isolation

    The pro-osteoclastic activity of norepinephrine (NE)-stimulated MSCs. The MSCs from 4-week experimental rats were stimulated with 1 μM NE for 2 h alone, or pre-treated for 1 h with 1 μM ICI 118,551 (ICI), 5 μM H-89 or 5 μM U-0126 (U0126) and then stimulated by NE. These MSCs were co-cultured with bone marrow macrophages. A : Representative images of TRAP-stained osteoclasts (upper row) and resorption pits (lower row) produced by the bone marrow marcrophages. B and C : Barcharts depicting the number of TRAP-positive multinucleated cells (10 fields) and area of resorption pits. D : Real-time PCR of the expression RANKL, OPG and RANKL/OPG ratio by MSCs isolated from the condylar subchondral bone of 4-week control rats (Con) and experimental rats (Exp) treated with physiological saline (Veh), propranolol (PRO) or isoproterenol (ISO).
    Figure Legend Snippet: The pro-osteoclastic activity of norepinephrine (NE)-stimulated MSCs. The MSCs from 4-week experimental rats were stimulated with 1 μM NE for 2 h alone, or pre-treated for 1 h with 1 μM ICI 118,551 (ICI), 5 μM H-89 or 5 μM U-0126 (U0126) and then stimulated by NE. These MSCs were co-cultured with bone marrow macrophages. A : Representative images of TRAP-stained osteoclasts (upper row) and resorption pits (lower row) produced by the bone marrow marcrophages. B and C : Barcharts depicting the number of TRAP-positive multinucleated cells (10 fields) and area of resorption pits. D : Real-time PCR of the expression RANKL, OPG and RANKL/OPG ratio by MSCs isolated from the condylar subchondral bone of 4-week control rats (Con) and experimental rats (Exp) treated with physiological saline (Veh), propranolol (PRO) or isoproterenol (ISO).

    Techniques Used: Activity Assay, Cell Culture, Staining, Produced, Real-time Polymerase Chain Reaction, Expressing, Isolation

    25) Product Images from "Biochanin A Promotes Osteogenic but Inhibits Adipogenic Differentiation: Evidence with Primary Adipose-Derived Stem Cells"

    Article Title: Biochanin A Promotes Osteogenic but Inhibits Adipogenic Differentiation: Evidence with Primary Adipose-Derived Stem Cells

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2013/846039

    Biochanin A enhanced the expression levels of genes and proteins that regulate osteogenic differentiation in ADSCs. ADSCs were treated for 12 days with a basic medium (negative control) or an osteogenic medium (OM) in the presence of 0.1–1 μ M biochanin A. (a) After incubation, expression of ALP, OCN, and GAPDH mRNA was measured by RT-PCR. Expression of ALP and OCN genes was normalized to that of GAPDH. (b) After incubation, total proteins were isolated and analyzed for the expression of OPG, Runx2, Sirt1, and RhoA proteins by western blot analysis. β -actin was used as the internal control. All results are expressed as the mean ± SD of three independent experiments. * P
    Figure Legend Snippet: Biochanin A enhanced the expression levels of genes and proteins that regulate osteogenic differentiation in ADSCs. ADSCs were treated for 12 days with a basic medium (negative control) or an osteogenic medium (OM) in the presence of 0.1–1 μ M biochanin A. (a) After incubation, expression of ALP, OCN, and GAPDH mRNA was measured by RT-PCR. Expression of ALP and OCN genes was normalized to that of GAPDH. (b) After incubation, total proteins were isolated and analyzed for the expression of OPG, Runx2, Sirt1, and RhoA proteins by western blot analysis. β -actin was used as the internal control. All results are expressed as the mean ± SD of three independent experiments. * P

    Techniques Used: Expressing, Negative Control, Incubation, ALP Assay, Reverse Transcription Polymerase Chain Reaction, Isolation, Western Blot

    26) Product Images from "Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis"

    Article Title: Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis

    Journal: Journal of Applied Oral Science

    doi: 10.1590/1678-7757-2020-0483

    Representative photomicrographs showing immunolabeling of the periapical regions (A-L). IL-1β, IL 6 and RANKL decreased in the PCG compared with those in the CG; IL-10 level was increased in the PCG; OPG level was similar in CG and PCG; TRAP-positive multinucleated cells count per mm in the periapical region the PCG obtained lower count compared with the CG. Original magnification: 400X. Charts showing scores for IL-10, IL-1β, IL 6, RANKL, OPG and TRAP
    Figure Legend Snippet: Representative photomicrographs showing immunolabeling of the periapical regions (A-L). IL-1β, IL 6 and RANKL decreased in the PCG compared with those in the CG; IL-10 level was increased in the PCG; OPG level was similar in CG and PCG; TRAP-positive multinucleated cells count per mm in the periapical region the PCG obtained lower count compared with the CG. Original magnification: 400X. Charts showing scores for IL-10, IL-1β, IL 6, RANKL, OPG and TRAP

    Techniques Used: Immunolabeling

    27) Product Images from "Specification of Sprouty2 functions in osteogenesis in in vivo context"

    Article Title: Specification of Sprouty2 functions in osteogenesis in in vivo context

    Journal: Organogenesis

    doi: 10.1080/15476278.2019.1656995

    Comparison of tibia in Spry2-/- and wt at E18 Expression of osteocalcin (A), sclerostin (B), Runx2 (C), Rank (D), Rankl (E), Opg (F), a tentative scheme of the molecular background of bone phenotype in Spry2-/- bones (G). Asterisk, p ≤ .05. Immunofluorescent detection of SOST (H, I) and RANK (J, K) in wild-type and Spry2-/- tibias, histology of the growth plate was shown after trichrome staining (L, N) and osteoclastic cells were detected by TRAP assay (M, O). PB (periosteal bone). For immunofluorescent labeling, positive signal is green, autofluorescent erythrocytes are orange in figures H, I. Positive signal is red, autofluorescent erythrocytes are green in figure J, K. Scale bar = 100 µm.
    Figure Legend Snippet: Comparison of tibia in Spry2-/- and wt at E18 Expression of osteocalcin (A), sclerostin (B), Runx2 (C), Rank (D), Rankl (E), Opg (F), a tentative scheme of the molecular background of bone phenotype in Spry2-/- bones (G). Asterisk, p ≤ .05. Immunofluorescent detection of SOST (H, I) and RANK (J, K) in wild-type and Spry2-/- tibias, histology of the growth plate was shown after trichrome staining (L, N) and osteoclastic cells were detected by TRAP assay (M, O). PB (periosteal bone). For immunofluorescent labeling, positive signal is green, autofluorescent erythrocytes are orange in figures H, I. Positive signal is red, autofluorescent erythrocytes are green in figure J, K. Scale bar = 100 µm.

    Techniques Used: Expressing, Staining, TRAP Assay, Labeling

    Immunohistochemical/immunofluorescent identification of osteogenic markers and SPRY2 in wild-type tibia at E18. Localization of osteoblastic cells identified as cuboidal cells with a single nucleus and expressing BGLAP (A), osteocytes entrapped in bone matrix and expressing SOST (B), and osteoclasts identified as large multinuclear cells positive for TRAP staining (C). Detection of SPRY2 in growth plate of tibia (D, E), with focus on resting, proliferating (F) and hypertrophic cartilage together with osteogenic zone (G). BGLAP (H) was localized in osteoblastic (H 1 ), and osteocytic cells (H 2 ), SOST (I) in osteocytes (I 1 ). In growth plate, RANK (J) was detected in osteoclastic cells (J 1 ), RANKL (K) in osteoblastic cells (K 1 ), OPG (L) in osteoblastic cells (L 1 ), and RUNX2 (M) in osteoblastic (M 1 ) and osteocytic cells (M 2 ). HZ (hypertrophic zone of cartilage), OZ (osteogenic zone), PB (periosteal bone), PS (primary spongiosa), PZ (proliferating zone of cartilage), RZ (resting zone of cartilage). For immunofluorescent labeling, positive signal is green, autofluorescent erythrocytes are orange in figures E-I, K-M. Positive signal is red, autofluorescent erythrocytes are green in figure J. Yellow arrowheads point to osteoblasts, green ones to osteocytes, and black ones to osteoclasts. Scale bar = 30 µm (A-C), 100 µm (E-M), 50 µm (H 1 -M 1 , H 2 , M 2 ).
    Figure Legend Snippet: Immunohistochemical/immunofluorescent identification of osteogenic markers and SPRY2 in wild-type tibia at E18. Localization of osteoblastic cells identified as cuboidal cells with a single nucleus and expressing BGLAP (A), osteocytes entrapped in bone matrix and expressing SOST (B), and osteoclasts identified as large multinuclear cells positive for TRAP staining (C). Detection of SPRY2 in growth plate of tibia (D, E), with focus on resting, proliferating (F) and hypertrophic cartilage together with osteogenic zone (G). BGLAP (H) was localized in osteoblastic (H 1 ), and osteocytic cells (H 2 ), SOST (I) in osteocytes (I 1 ). In growth plate, RANK (J) was detected in osteoclastic cells (J 1 ), RANKL (K) in osteoblastic cells (K 1 ), OPG (L) in osteoblastic cells (L 1 ), and RUNX2 (M) in osteoblastic (M 1 ) and osteocytic cells (M 2 ). HZ (hypertrophic zone of cartilage), OZ (osteogenic zone), PB (periosteal bone), PS (primary spongiosa), PZ (proliferating zone of cartilage), RZ (resting zone of cartilage). For immunofluorescent labeling, positive signal is green, autofluorescent erythrocytes are orange in figures E-I, K-M. Positive signal is red, autofluorescent erythrocytes are green in figure J. Yellow arrowheads point to osteoblasts, green ones to osteocytes, and black ones to osteoclasts. Scale bar = 30 µm (A-C), 100 µm (E-M), 50 µm (H 1 -M 1 , H 2 , M 2 ).

    Techniques Used: Immunohistochemistry, Expressing, Staining, Labeling

    28) Product Images from "The expression of cystathionine gamma-lyase is regulated by estrogen receptor alpha in human osteoblasts"

    Article Title: The expression of cystathionine gamma-lyase is regulated by estrogen receptor alpha in human osteoblasts

    Journal: Oncotarget

    doi: 10.18632/oncotarget.21514

    CSE protein expression in hOBs from male (M) and female (F) donors (A) The cells were harvested after 72 hours of culture and subjected to Western blot analysis for CSE expression. Bar graphs show the densitometric analysis of all samples analyzed. GAPDH expression was used as the internal control to evaluate total protein of samples loaded, data were expressed as ratio of CSE in respect to GAPDH and presented as mean + standard deviation, SD (n=3 for male group, n=3 for female group). (B) The cells were exposed to ERα overexpression together 10 nM 17β-estradiol for 48 hours (+ ERα) or remained untreated (-) and subjected to Western blot analysis for ERα, CSE and OPG expression. Representative Western blot is reported (sample 2, M2, for male group, and sample 2, F2, for female group). Bar graphs show the densitometric analysis of all samples analyzed. GAPDH expression was used as the internal control to evaluate total protein of samples loaded, data were expressed as ratio of ERα, CSE and OPG in respect to GAPDH and presented as mean + standard deviation, SD (n=3 for male group, n=3 for female group).
    Figure Legend Snippet: CSE protein expression in hOBs from male (M) and female (F) donors (A) The cells were harvested after 72 hours of culture and subjected to Western blot analysis for CSE expression. Bar graphs show the densitometric analysis of all samples analyzed. GAPDH expression was used as the internal control to evaluate total protein of samples loaded, data were expressed as ratio of CSE in respect to GAPDH and presented as mean + standard deviation, SD (n=3 for male group, n=3 for female group). (B) The cells were exposed to ERα overexpression together 10 nM 17β-estradiol for 48 hours (+ ERα) or remained untreated (-) and subjected to Western blot analysis for ERα, CSE and OPG expression. Representative Western blot is reported (sample 2, M2, for male group, and sample 2, F2, for female group). Bar graphs show the densitometric analysis of all samples analyzed. GAPDH expression was used as the internal control to evaluate total protein of samples loaded, data were expressed as ratio of ERα, CSE and OPG in respect to GAPDH and presented as mean + standard deviation, SD (n=3 for male group, n=3 for female group).

    Techniques Used: Expressing, Western Blot, Standard Deviation, Over Expression

    29) Product Images from "Expression of Osteoprotegrin and Osteoclast Level in Chronic Apical Periodontitis Induced with East Java Propolis Extract"

    Article Title: Expression of Osteoprotegrin and Osteoclast Level in Chronic Apical Periodontitis Induced with East Java Propolis Extract

    Journal: Iranian Endodontic Journal

    doi: 10.22037/iej.v13i1.18781

    OPG Expression in periapical Wistar rats. A) The oval section represents a periapical area at 400× magnification in Control group; B) E. faecalis group; C) Propolis group; D, E, F) Under 1000× magnification then was conducted to confirm the oval area
    Figure Legend Snippet: OPG Expression in periapical Wistar rats. A) The oval section represents a periapical area at 400× magnification in Control group; B) E. faecalis group; C) Propolis group; D, E, F) Under 1000× magnification then was conducted to confirm the oval area

    Techniques Used: Expressing

    30) Product Images from "Senescence: novel insight into DLX3 mutations leading to enhanced bone formation in Tricho-Dento-Osseous syndrome"

    Article Title: Senescence: novel insight into DLX3 mutations leading to enhanced bone formation in Tricho-Dento-Osseous syndrome

    Journal: Scientific Reports

    doi: 10.1038/srep38680

    TDO-BMSCs exhibit weaker osteogenic potential than CON-BMSCs. TDO- and CON-BMSCs at passage 3 were cultured under osteoinduction medium. ( A ) ALP staining assay on days 3, 7, and 14 after osteoinduction. ( B ) Alizarin red staining assay at 14 and 21 days post-osteoinduction. ( C ) ALP activity assay on days 3, 7, and 14 after osteoinduction. ( D ) Quantification of alizarin red staining on days 14 and 21 post-osteoinduction. ( E ) Western blots of RUNX2, BSP, RANKL, OPG, and β-actin protein 14 days after osteogenic stimulation. ( F ) Osteogenesis-related genes ( RUNX2, ALP, COLLAGEN I, BSP, OCN, RANKL and OPG) mRNA expression after osteoinduction. GAPDH served as an internal control. Data were presented as the mean ± S.D. of 3 independent experiments. *p
    Figure Legend Snippet: TDO-BMSCs exhibit weaker osteogenic potential than CON-BMSCs. TDO- and CON-BMSCs at passage 3 were cultured under osteoinduction medium. ( A ) ALP staining assay on days 3, 7, and 14 after osteoinduction. ( B ) Alizarin red staining assay at 14 and 21 days post-osteoinduction. ( C ) ALP activity assay on days 3, 7, and 14 after osteoinduction. ( D ) Quantification of alizarin red staining on days 14 and 21 post-osteoinduction. ( E ) Western blots of RUNX2, BSP, RANKL, OPG, and β-actin protein 14 days after osteogenic stimulation. ( F ) Osteogenesis-related genes ( RUNX2, ALP, COLLAGEN I, BSP, OCN, RANKL and OPG) mRNA expression after osteoinduction. GAPDH served as an internal control. Data were presented as the mean ± S.D. of 3 independent experiments. *p

    Techniques Used: Cell Culture, ALP Assay, Staining, Activity Assay, Western Blot, Expressing

    31) Product Images from "ZhiJingSan Inhibits Osteoclastogenesis via Regulating RANKL/NF-κB Signaling Pathway and Ameliorates Bone Erosion in Collagen-Induced Mouse Arthritis"

    Article Title: ZhiJingSan Inhibits Osteoclastogenesis via Regulating RANKL/NF-κB Signaling Pathway and Ameliorates Bone Erosion in Collagen-Induced Mouse Arthritis

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2021.693777

    ZJS decreased the number of osteoclasts and the expression of osteoclast-related proteins in the ankle joints of CIA mice. (A) Representative immunohistochemical staining images of TRAP, cathepsin K, OPG, and RANKL in the ankle joints of mice in the indicated groups (scales bars = 100 μm). (B) The quantification of cathepsin K, RANKL, and OPG expression in the ankle joints of mice in the indicated groups ( n = 3). (C) The number of TRAP-positive cells in the ankle joints in the indicated groups ( n = 5). (D) The content of RANKL in the serum of mice in the indicated groups ( n = 5). (E) Ratio of the IHC intensity of OPG and RANKL in the ankle joints in the indicated groups ( n = 3). Values are presented as the mean ± SEM. ns: no significance. ## p
    Figure Legend Snippet: ZJS decreased the number of osteoclasts and the expression of osteoclast-related proteins in the ankle joints of CIA mice. (A) Representative immunohistochemical staining images of TRAP, cathepsin K, OPG, and RANKL in the ankle joints of mice in the indicated groups (scales bars = 100 μm). (B) The quantification of cathepsin K, RANKL, and OPG expression in the ankle joints of mice in the indicated groups ( n = 3). (C) The number of TRAP-positive cells in the ankle joints in the indicated groups ( n = 5). (D) The content of RANKL in the serum of mice in the indicated groups ( n = 5). (E) Ratio of the IHC intensity of OPG and RANKL in the ankle joints in the indicated groups ( n = 3). Values are presented as the mean ± SEM. ns: no significance. ## p

    Techniques Used: Expressing, Mouse Assay, Immunohistochemistry, Staining

    32) Product Images from "Manganese superoxide dismutase is required to maintain osteoclast differentiation and function under static force"

    Article Title: Manganese superoxide dismutase is required to maintain osteoclast differentiation and function under static force

    Journal: Scientific Reports

    doi: 10.1038/srep08016

    Identification of induced osteoclasts. (A), Analyses of cell surface markers were performed via flow cytometry detecting PE, FITC or PerCP conjugated monoclonal antibodies for human CD11b, CD14, CD34, CD45, CD105, CD146, or isotypematched control IgGs. (B), HMNCs induced for 12 d with 1α, 25-(OH) 2 D 3 (10 −8 M) showing matured to TRAP positive (red staining) multinuclear cells. Giemsa staining (blue) clearly showed multinuclear morphologies of matured OCs. Bone resorption pits were analysed by Toluidine-blue staining. No resorption pits were formed. Magnification: ×100. (C), The expressions of CTSK, TRAP, CTR, MMP-9, OPG and RANKL were analyzed by real-time PCR and western blotting. Cont, control; MNCs, human cord monocytes; OCs, osteoclasts. BPs, Bone resorption pits. Data represent mean ± SD of the relative ratio of the gene signal to the β-actin signal. *P
    Figure Legend Snippet: Identification of induced osteoclasts. (A), Analyses of cell surface markers were performed via flow cytometry detecting PE, FITC or PerCP conjugated monoclonal antibodies for human CD11b, CD14, CD34, CD45, CD105, CD146, or isotypematched control IgGs. (B), HMNCs induced for 12 d with 1α, 25-(OH) 2 D 3 (10 −8 M) showing matured to TRAP positive (red staining) multinuclear cells. Giemsa staining (blue) clearly showed multinuclear morphologies of matured OCs. Bone resorption pits were analysed by Toluidine-blue staining. No resorption pits were formed. Magnification: ×100. (C), The expressions of CTSK, TRAP, CTR, MMP-9, OPG and RANKL were analyzed by real-time PCR and western blotting. Cont, control; MNCs, human cord monocytes; OCs, osteoclasts. BPs, Bone resorption pits. Data represent mean ± SD of the relative ratio of the gene signal to the β-actin signal. *P

    Techniques Used: Flow Cytometry, Cytometry, Staining, Real-time Polymerase Chain Reaction, Western Blot

    33) Product Images from "Combined effect of recombinant human bone morphogenetic protein-2 and low level laser irradiation on bisphosphonate-treated osteoblasts"

    Article Title: Combined effect of recombinant human bone morphogenetic protein-2 and low level laser irradiation on bisphosphonate-treated osteoblasts

    Journal: Journal of the Korean Association of Oral and Maxillofacial Surgeons

    doi: 10.5125/jkaoms.2018.44.6.259

    Effects of alendronate, recombinant human bone morphogenetic protein-2 (rhBMP-2), and low-level laser therapy (LLLT) on receptor activator of nuclear factor kappa-B (RANKL), osteoprotegerin (OPG), and macrophage colony-stimulating factor (M-CSF) expression as determined by real-time polymerase chain reaction. A. RANKL expression. B. OPG expression. C. M-CSF expression. Treatment with rhBMP-2 resulted in greater effects on OPG and M-CSF expression, while treatment with LLLT had an effect on expression of RANKL. However, the differences between the effects of rhBMP-2 and LLLT were not statistically significant. * P
    Figure Legend Snippet: Effects of alendronate, recombinant human bone morphogenetic protein-2 (rhBMP-2), and low-level laser therapy (LLLT) on receptor activator of nuclear factor kappa-B (RANKL), osteoprotegerin (OPG), and macrophage colony-stimulating factor (M-CSF) expression as determined by real-time polymerase chain reaction. A. RANKL expression. B. OPG expression. C. M-CSF expression. Treatment with rhBMP-2 resulted in greater effects on OPG and M-CSF expression, while treatment with LLLT had an effect on expression of RANKL. However, the differences between the effects of rhBMP-2 and LLLT were not statistically significant. * P

    Techniques Used: Recombinant, Expressing, Real-time Polymerase Chain Reaction

    34) Product Images from "Total glucosides of paeony prevents juxta-articular bone loss in experimental arthritis"

    Article Title: Total glucosides of paeony prevents juxta-articular bone loss in experimental arthritis

    Journal: BMC Complementary and Alternative Medicine

    doi: 10.1186/1472-6882-13-186

    TGP treatment decreases RANKL expression and increases OPG expression. (A) RANKL mRNA and (B) OPG mRNA expression in each experimental group. (C) RANKL/OPG ratio. Results are presented as mean ± S.D.
    Figure Legend Snippet: TGP treatment decreases RANKL expression and increases OPG expression. (A) RANKL mRNA and (B) OPG mRNA expression in each experimental group. (C) RANKL/OPG ratio. Results are presented as mean ± S.D.

    Techniques Used: Expressing

    RANKL and OPG expression in articular cartilage and subchondral bone. Histological sections were submitted for immunohistochemical analyses. RANKL staining is shown in each group in Figure 4 (A-D) : (A) Normal group. RANKL immunolabeling was rarely detected. (B) AIA group. RANKL immunolabeling was detected at high levels, especially in subchondral bone. (C) TGP group. TGP decreased RANKL expression. (D) IND group. OPG staining for each group is shown in Figure 4 (E-H) : (E) Normal group. OPG was expressed only in deep zone. (F) AIA group (G) TGP group. OPG was highly expressed in subchondral bone. (H) IND group (original magnification × 400).
    Figure Legend Snippet: RANKL and OPG expression in articular cartilage and subchondral bone. Histological sections were submitted for immunohistochemical analyses. RANKL staining is shown in each group in Figure 4 (A-D) : (A) Normal group. RANKL immunolabeling was rarely detected. (B) AIA group. RANKL immunolabeling was detected at high levels, especially in subchondral bone. (C) TGP group. TGP decreased RANKL expression. (D) IND group. OPG staining for each group is shown in Figure 4 (E-H) : (E) Normal group. OPG was expressed only in deep zone. (F) AIA group (G) TGP group. OPG was highly expressed in subchondral bone. (H) IND group (original magnification × 400).

    Techniques Used: Expressing, Immunohistochemistry, Staining, Immunolabeling

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    Incubation:

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    Flow Cytometry:

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    Immunohistochemistry:

    Article Title: Expression of Osteoprotegrin and Osteoclast Level in Chronic Apical Periodontitis Induced with East Java Propolis Extract
    Article Snippet: A period of twenty-one days was required to induce chronic apical periodontitis after pulp infection [ ]. .. Wistar rats were sacrificed for immunohistochemical examination to enable measurement of the expression of OPG (Biotechnology, Santa Cruz, USA) under 400× magnification and histochemical examination with Hematoxillin Eosin also under 400× magnification to measure the osteoclast cells. ..

    Article Title: Uncommon presentation of potential medication-related osteonecrosis of the jaw
    Article Snippet: .. The microsections were routinely stained with hematoxylin and eosin (HE), and we also performed immunohistochemical (IHC) staining using anti-sera of lysozyme (DAKO, Glostrup, Denmark), matrix metalloprotease-1 (MMP-1, Santa Cruz Biotech, Santa Cruz, CA, USA), MMP-2 (Santa Cruz Biotech), MMP-3 (Santa Cruz Biotech), hypoxia inducible protein alpha (HIFα; Abcam, Cambridge, UK), vascular endothelial cell growth factor (VEGF; Abcam), osteoprotegerin (OPG; Santa Cruz Biotech), and the receptor activator of nuclear factor-kappa B ligand (RANKL; Santa Cruz Biotech). .. IHC staining was performed using the indirect triple sandwich method.

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  • 94
    Santa Cruz Biotechnology opg antibody
    ABE regulates the expression of <t>RANKL</t> and <t>OPG</t> in bone marrow mesenchymal stem cells (BMSCs). BMSCs were cultured with or without different concentrations of ABE (0.16, 0.8, 4 μg/ml, respectively). Three days post-culture, supernatants were obtained to detectthe amounts of RANKL (A) and OPG (B) in the supernatants by ELISA. (C) refers to the ratio of RANKL/OPG in the supernatants. Data are represented as the mean ± SD of three independent experiments. *P
    Opg Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/opg antibody/product/Santa Cruz Biotechnology
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    opg antibody - by Bioz Stars, 2021-07
    94/100 stars
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    93
    Santa Cruz Biotechnology opg
    LLDT-8 increased the ratio of <t>OPG/RANKL</t> in synovial fluid of RA patients. SFMCs were isolated from synovial fluid of RA patients and treated with various concentrations of LLDT-8 (0, 25 and 50 nM, respectively) for 24 hours, then stained and detected by flow cytometry. (A) The psuedocolor plots of OPG, RANK and RANKL. (B) The rate of OPG expression on <t>CD3</t> + T leukomonocyte in the three groups. (C) The ratio of OPG/RANKL on CD3 + T cells in the three groups. * p
    Opg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/opg/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    opg - by Bioz Stars, 2021-07
    93/100 stars
      Buy from Supplier

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    Gene Silencers generally consist of pools of three to five target specific 19 25 nucleotide sequences in length For independent verification of OPG gene silencing results individual duplex components or
      Buy from Supplier

    N/A
    Gene Silencers generally consist of pools of three to five target specific 19 25 nucleotide sequences in length For independent verification of OPG gene silencing results individual duplex components or
      Buy from Supplier

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    ABE regulates the expression of RANKL and OPG in bone marrow mesenchymal stem cells (BMSCs). BMSCs were cultured with or without different concentrations of ABE (0.16, 0.8, 4 μg/ml, respectively). Three days post-culture, supernatants were obtained to detectthe amounts of RANKL (A) and OPG (B) in the supernatants by ELISA. (C) refers to the ratio of RANKL/OPG in the supernatants. Data are represented as the mean ± SD of three independent experiments. *P

    Journal: Journal of Translational Medicine

    Article Title: Achyranthes bidentata extract exerts osteoprotective effects on steroid-induced osteonecrosis of the femoral head in rats by regulating RANKL/RANK/OPG signaling

    doi: 10.1186/s12967-014-0334-7

    Figure Lengend Snippet: ABE regulates the expression of RANKL and OPG in bone marrow mesenchymal stem cells (BMSCs). BMSCs were cultured with or without different concentrations of ABE (0.16, 0.8, 4 μg/ml, respectively). Three days post-culture, supernatants were obtained to detectthe amounts of RANKL (A) and OPG (B) in the supernatants by ELISA. (C) refers to the ratio of RANKL/OPG in the supernatants. Data are represented as the mean ± SD of three independent experiments. *P

    Article Snippet: The following antibodies were used: RANK antibody (rabbit antibody, dilution 1:50, Cell Signaling Technology, Inc., Danvers, MA, USA), RANKL antibody (rabbit antibody, dilution 1:100, Millipore Corporation, Billerica, MA, USA), OPG antibody (rabbit antibody, dilution 1:100, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), and GAPDH antibody (internal control, rabbit polyclonal antibody, dilution 1:200, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA).

    Techniques: Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay

    ABE treatment regulates the RANKL/RANK/OPG signaling pathway in rats with steroid-induced ONFH. RANK, RANKL, and OPG levels in the serum of rats with steroid-induced ONFH with or without ABE treatment were detected by ELISA (A) . RANKL, RANK, and OPG expression in the femoral heads of rats with steroid-induced ONFH with or without ABE treatment were detected at mRNA and protein levels by quantitative real-time RT-PCR (B) , western blot (C) , respectively. (D) shows the ratio of RANKL/OPG in the serum, the ratio of RANKL mRNA/OPG mRNA, and the ratio of RANK protein/OPG protein in the rats with steroid-induced ONFH with and without ABE treatment. Data are presented as the mean ± S.D. (n =20 for control, n =25 for model, n =20 for ABE treatment groups). ##: P

    Journal: Journal of Translational Medicine

    Article Title: Achyranthes bidentata extract exerts osteoprotective effects on steroid-induced osteonecrosis of the femoral head in rats by regulating RANKL/RANK/OPG signaling

    doi: 10.1186/s12967-014-0334-7

    Figure Lengend Snippet: ABE treatment regulates the RANKL/RANK/OPG signaling pathway in rats with steroid-induced ONFH. RANK, RANKL, and OPG levels in the serum of rats with steroid-induced ONFH with or without ABE treatment were detected by ELISA (A) . RANKL, RANK, and OPG expression in the femoral heads of rats with steroid-induced ONFH with or without ABE treatment were detected at mRNA and protein levels by quantitative real-time RT-PCR (B) , western blot (C) , respectively. (D) shows the ratio of RANKL/OPG in the serum, the ratio of RANKL mRNA/OPG mRNA, and the ratio of RANK protein/OPG protein in the rats with steroid-induced ONFH with and without ABE treatment. Data are presented as the mean ± S.D. (n =20 for control, n =25 for model, n =20 for ABE treatment groups). ##: P

    Article Snippet: The following antibodies were used: RANK antibody (rabbit antibody, dilution 1:50, Cell Signaling Technology, Inc., Danvers, MA, USA), RANKL antibody (rabbit antibody, dilution 1:100, Millipore Corporation, Billerica, MA, USA), OPG antibody (rabbit antibody, dilution 1:100, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), and GAPDH antibody (internal control, rabbit polyclonal antibody, dilution 1:200, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA).

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Western Blot

    Induction of RANKL expression by TiPs. (A) The gene expression of RANKL and OPG in TiPs-treated fibroblasts were examined by real-time PCR. (B) Western blots analysis of RANKL and OPG in fibroblasts following treatment with TiPs for various time periods. (C) The density of western blots bands shown in (B) was quantified using the ImageJ software. (D and E) The expression of sRANKL in the supernatants from each group were quantified by ELISA. Data are represented as the means ± S.E.M from three independent experiments. (A and C) *P

    Journal: PLoS ONE

    Article Title: ER Stress Mediates TiAl6V4 Particle-Induced Peri-Implant Osteolysis by Promoting RANKL Expression in Fibroblasts

    doi: 10.1371/journal.pone.0137774

    Figure Lengend Snippet: Induction of RANKL expression by TiPs. (A) The gene expression of RANKL and OPG in TiPs-treated fibroblasts were examined by real-time PCR. (B) Western blots analysis of RANKL and OPG in fibroblasts following treatment with TiPs for various time periods. (C) The density of western blots bands shown in (B) was quantified using the ImageJ software. (D and E) The expression of sRANKL in the supernatants from each group were quantified by ELISA. Data are represented as the means ± S.E.M from three independent experiments. (A and C) *P

    Article Snippet: Western blot was performed using the following primary antibodies: anti-inositol-requiring kinase 1 (IRE1-a), anti-glucose-regulated protein 78 (GRP/Bip), anti-C/EBP homologous protein (CHOP), and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Cell Signaling Technology, Beverly, MA, USA); anti-receptor activator of nuclear factor kappa-B ligand (RANKL) and anti- osteoprotegrin (OPG) (Santa Cruz, CA, USA); subsequently, the following secondary antibodies were applied: horseradish peroxidase-conjugated anti-rabbit IgG (Cell Signaling Technology) and horseradish peroxidase-conjugated anti-mouse IgG (Santa Cruz, CA, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Software, Enzyme-linked Immunosorbent Assay

    ER stress mediated the upregulation of RANKL and the RANKL/OPG ratio in TiPs-stimulated fibroblasts. (A and B) The gene expression of RANKL and OPG in fibroblasts from each group were examined by real-time PCR. (C, E and G) Western blots analysis of RANKL and OPG from each group. (D, F and H) The density of western blots bands shown in (C, E and G) was quantified using the ImageJ software. (I) The expression of sRANKL in the supernatants of fibroblasts from each group were quantified by ELISA. Data are represented as the means ± S.E.M from three independent experiments. *P

    Journal: PLoS ONE

    Article Title: ER Stress Mediates TiAl6V4 Particle-Induced Peri-Implant Osteolysis by Promoting RANKL Expression in Fibroblasts

    doi: 10.1371/journal.pone.0137774

    Figure Lengend Snippet: ER stress mediated the upregulation of RANKL and the RANKL/OPG ratio in TiPs-stimulated fibroblasts. (A and B) The gene expression of RANKL and OPG in fibroblasts from each group were examined by real-time PCR. (C, E and G) Western blots analysis of RANKL and OPG from each group. (D, F and H) The density of western blots bands shown in (C, E and G) was quantified using the ImageJ software. (I) The expression of sRANKL in the supernatants of fibroblasts from each group were quantified by ELISA. Data are represented as the means ± S.E.M from three independent experiments. *P

    Article Snippet: Western blot was performed using the following primary antibodies: anti-inositol-requiring kinase 1 (IRE1-a), anti-glucose-regulated protein 78 (GRP/Bip), anti-C/EBP homologous protein (CHOP), and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Cell Signaling Technology, Beverly, MA, USA); anti-receptor activator of nuclear factor kappa-B ligand (RANKL) and anti- osteoprotegrin (OPG) (Santa Cruz, CA, USA); subsequently, the following secondary antibodies were applied: horseradish peroxidase-conjugated anti-rabbit IgG (Cell Signaling Technology) and horseradish peroxidase-conjugated anti-mouse IgG (Santa Cruz, CA, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Software, Enzyme-linked Immunosorbent Assay

    Expression of receptor activator of nuclear factor κB ligand (RANKL) and osteoprotegerin (OPG) mRNA in alveolar bone cells (ABCs) and dental pulp cells (DPCs). ( A ) Phase-contrast image of ABCs isolated from green fluorescent protein (GFP) rats

    Journal: Journal of Dental Research

    Article Title: Mesenchymal Dental Pulp Cells Attenuate Dentin Resorption in Homeostasis

    doi: 10.1177/0022034515575347

    Figure Lengend Snippet: Expression of receptor activator of nuclear factor κB ligand (RANKL) and osteoprotegerin (OPG) mRNA in alveolar bone cells (ABCs) and dental pulp cells (DPCs). ( A ) Phase-contrast image of ABCs isolated from green fluorescent protein (GFP) rats

    Article Snippet: The antibodies were goat anti-RANKL polyclonal antibody and anti-OPG a polyclonal antibody (sc-7628 and sc-8468; 1:200 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA).

    Techniques: Expressing, Isolation

    LLDT-8 increased the ratio of OPG/RANKL in synovial fluid of RA patients. SFMCs were isolated from synovial fluid of RA patients and treated with various concentrations of LLDT-8 (0, 25 and 50 nM, respectively) for 24 hours, then stained and detected by flow cytometry. (A) The psuedocolor plots of OPG, RANK and RANKL. (B) The rate of OPG expression on CD3 + T leukomonocyte in the three groups. (C) The ratio of OPG/RANKL on CD3 + T cells in the three groups. * p

    Journal: BMC Complementary and Alternative Medicine

    Article Title: (5R)-5-Hydroxytriptolide (LLDT-8) inhibits osteoclastogenesis via RANKL/RANK/OPG signaling pathway

    doi: 10.1186/s12906-015-0566-y

    Figure Lengend Snippet: LLDT-8 increased the ratio of OPG/RANKL in synovial fluid of RA patients. SFMCs were isolated from synovial fluid of RA patients and treated with various concentrations of LLDT-8 (0, 25 and 50 nM, respectively) for 24 hours, then stained and detected by flow cytometry. (A) The psuedocolor plots of OPG, RANK and RANKL. (B) The rate of OPG expression on CD3 + T leukomonocyte in the three groups. (C) The ratio of OPG/RANKL on CD3 + T cells in the three groups. * p

    Article Snippet: The cells were harvested and washed with FACS washing buffer followed by incubation with anti-human CD3 (eBioscience, USA) and OPG (bony-1, SANTA CRUZ Biotec, USA) at 4°C for 20 min. After washed by FACS washing buffer, the cells were incubated with goat anti-rat IgG-FITC (SANTA CRUZ) at 4°C for 20 min, then PE conjugated anti-human RANK (R & D Systems, USA), APC conjugated anti-human RANKL (R & D Systems) and Percp conjugated anti-human CD3 (Miltenyi Biotec, Germany) were added respectively, and incubated at 4°C for 20 min. Then the cells were detected by flow cytometry.

    Techniques: Isolation, Staining, Flow Cytometry, Cytometry, Expressing

    LLDT-8 up-regulated OPG expression and increased the ratio of OPG/RANKL on CD3 + T leukomonocyte in peripheral blood of RA patients. PBMCs were isolated from peripheral blood of RA patients and treated with various concentrations of LLDT-8 (0, 25 nM and 50 nM, respectively) for 24 hours, then stained and detected by flow cytometry. (A) The psuedocolor plots of OPG, RANK and RANKL. (B) The rate of OPG expression on CD3 + T leukomonocyte in the three groups. (C) The ratio of OPG to RANKL on CD3 + T leukomonocyte in the three groups. * p

    Journal: BMC Complementary and Alternative Medicine

    Article Title: (5R)-5-Hydroxytriptolide (LLDT-8) inhibits osteoclastogenesis via RANKL/RANK/OPG signaling pathway

    doi: 10.1186/s12906-015-0566-y

    Figure Lengend Snippet: LLDT-8 up-regulated OPG expression and increased the ratio of OPG/RANKL on CD3 + T leukomonocyte in peripheral blood of RA patients. PBMCs were isolated from peripheral blood of RA patients and treated with various concentrations of LLDT-8 (0, 25 nM and 50 nM, respectively) for 24 hours, then stained and detected by flow cytometry. (A) The psuedocolor plots of OPG, RANK and RANKL. (B) The rate of OPG expression on CD3 + T leukomonocyte in the three groups. (C) The ratio of OPG to RANKL on CD3 + T leukomonocyte in the three groups. * p

    Article Snippet: The cells were harvested and washed with FACS washing buffer followed by incubation with anti-human CD3 (eBioscience, USA) and OPG (bony-1, SANTA CRUZ Biotec, USA) at 4°C for 20 min. After washed by FACS washing buffer, the cells were incubated with goat anti-rat IgG-FITC (SANTA CRUZ) at 4°C for 20 min, then PE conjugated anti-human RANK (R & D Systems, USA), APC conjugated anti-human RANKL (R & D Systems) and Percp conjugated anti-human CD3 (Miltenyi Biotec, Germany) were added respectively, and incubated at 4°C for 20 min. Then the cells were detected by flow cytometry.

    Techniques: Expressing, Isolation, Staining, Flow Cytometry, Cytometry