opg antibody  (Santa Cruz Biotechnology)

 
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    Name:
    OPG Antibody
    Description:
    Anti OPG Antibody E 10 is a mouse monoclonal IgG1 kappa light chain OPG antibody provided at 200 µg ml raised against amino acids 153 401 of OPG of human origin Anti OPG Antibody E 10 is recommended for detection of OPG of mouse rat and human origin by WB IP IF and ELISA Anti OPG Antibody E 10 is available conjugated to agarose for IP HRP for WB IHC P and ELISA and to either phycoerythrin or FITC for IF IHC P and FCM also available conjugated to Alexa Fluor 488 Alexa Fluor 546 Alexa Fluor 594 or Alexa Fluor 647 for WB RGB IF IHC P and FCM and for use with RGB fluorescent imaging systems such as iBright FL1000 FluorChem Typhoon Azure and other comparable systems also available conjugated to Alexa Fluor 680 or Alexa Fluor 790 for WB NIR IF and FCM for use with Near Infrared NIR detection systems such as LI COR Odyssey iBright FL1000 FluorChem Typhoon Azure and other comparable systems TransCruz reagent for ChIP application sc 390518 X 200 µg 0 1 ml Contact our Technical Service Department or your local Distributor for more information on how to receive a FREE 10 µg sample of OPG E 10 sc 390518
    Catalog Number:
    SC-390518
    Price:
    None
    Category:
    Antibodies Primary Antibodies and ImmunoCruz Conjugates Membrane Receptors OPG Antibodies OPG Antibody E 10
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    Structured Review

    Santa Cruz Biotechnology opg antibody
    ABE regulates the expression of <t>RANKL</t> and <t>OPG</t> in bone marrow mesenchymal stem cells (BMSCs). BMSCs were cultured with or without different concentrations of ABE (0.16, 0.8, 4 μg/ml, respectively). Three days post-culture, supernatants were obtained to detectthe amounts of RANKL (A) and OPG (B) in the supernatants by ELISA. (C) refers to the ratio of RANKL/OPG in the supernatants. Data are represented as the mean ± SD of three independent experiments. *P
    Anti OPG Antibody E 10 is a mouse monoclonal IgG1 kappa light chain OPG antibody provided at 200 µg ml raised against amino acids 153 401 of OPG of human origin Anti OPG Antibody E 10 is recommended for detection of OPG of mouse rat and human origin by WB IP IF and ELISA Anti OPG Antibody E 10 is available conjugated to agarose for IP HRP for WB IHC P and ELISA and to either phycoerythrin or FITC for IF IHC P and FCM also available conjugated to Alexa Fluor 488 Alexa Fluor 546 Alexa Fluor 594 or Alexa Fluor 647 for WB RGB IF IHC P and FCM and for use with RGB fluorescent imaging systems such as iBright FL1000 FluorChem Typhoon Azure and other comparable systems also available conjugated to Alexa Fluor 680 or Alexa Fluor 790 for WB NIR IF and FCM for use with Near Infrared NIR detection systems such as LI COR Odyssey iBright FL1000 FluorChem Typhoon Azure and other comparable systems TransCruz reagent for ChIP application sc 390518 X 200 µg 0 1 ml Contact our Technical Service Department or your local Distributor for more information on how to receive a FREE 10 µg sample of OPG E 10 sc 390518
    https://www.bioz.com/result/opg antibody/product/Santa Cruz Biotechnology
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    opg antibody - by Bioz Stars, 2021-09
    98/100 stars

    Images

    1) Product Images from "Achyranthes bidentata extract exerts osteoprotective effects on steroid-induced osteonecrosis of the femoral head in rats by regulating RANKL/RANK/OPG signaling"

    Article Title: Achyranthes bidentata extract exerts osteoprotective effects on steroid-induced osteonecrosis of the femoral head in rats by regulating RANKL/RANK/OPG signaling

    Journal: Journal of Translational Medicine

    doi: 10.1186/s12967-014-0334-7

    ABE regulates the expression of RANKL and OPG in bone marrow mesenchymal stem cells (BMSCs). BMSCs were cultured with or without different concentrations of ABE (0.16, 0.8, 4 μg/ml, respectively). Three days post-culture, supernatants were obtained to detectthe amounts of RANKL (A) and OPG (B) in the supernatants by ELISA. (C) refers to the ratio of RANKL/OPG in the supernatants. Data are represented as the mean ± SD of three independent experiments. *P
    Figure Legend Snippet: ABE regulates the expression of RANKL and OPG in bone marrow mesenchymal stem cells (BMSCs). BMSCs were cultured with or without different concentrations of ABE (0.16, 0.8, 4 μg/ml, respectively). Three days post-culture, supernatants were obtained to detectthe amounts of RANKL (A) and OPG (B) in the supernatants by ELISA. (C) refers to the ratio of RANKL/OPG in the supernatants. Data are represented as the mean ± SD of three independent experiments. *P

    Techniques Used: Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay

    ABE treatment regulates the RANKL/RANK/OPG signaling pathway in rats with steroid-induced ONFH. RANK, RANKL, and OPG levels in the serum of rats with steroid-induced ONFH with or without ABE treatment were detected by ELISA (A) . RANKL, RANK, and OPG expression in the femoral heads of rats with steroid-induced ONFH with or without ABE treatment were detected at mRNA and protein levels by quantitative real-time RT-PCR (B) , western blot (C) , respectively. (D) shows the ratio of RANKL/OPG in the serum, the ratio of RANKL mRNA/OPG mRNA, and the ratio of RANK protein/OPG protein in the rats with steroid-induced ONFH with and without ABE treatment. Data are presented as the mean ± S.D. (n =20 for control, n =25 for model, n =20 for ABE treatment groups). ##: P
    Figure Legend Snippet: ABE treatment regulates the RANKL/RANK/OPG signaling pathway in rats with steroid-induced ONFH. RANK, RANKL, and OPG levels in the serum of rats with steroid-induced ONFH with or without ABE treatment were detected by ELISA (A) . RANKL, RANK, and OPG expression in the femoral heads of rats with steroid-induced ONFH with or without ABE treatment were detected at mRNA and protein levels by quantitative real-time RT-PCR (B) , western blot (C) , respectively. (D) shows the ratio of RANKL/OPG in the serum, the ratio of RANKL mRNA/OPG mRNA, and the ratio of RANK protein/OPG protein in the rats with steroid-induced ONFH with and without ABE treatment. Data are presented as the mean ± S.D. (n =20 for control, n =25 for model, n =20 for ABE treatment groups). ##: P

    Techniques Used: Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Western Blot

    2) Product Images from "Quercetin Induces Anticancer Activity by Upregulating Pro-NAG-1/GDF15 in Differentiated Thyroid Cancer Cells"

    Article Title: Quercetin Induces Anticancer Activity by Upregulating Pro-NAG-1/GDF15 in Differentiated Thyroid Cancer Cells

    Journal: Cancers

    doi: 10.3390/cancers13123022

    Antibody array using human thyroid tissues. ( A ) Antibody array (RayBio ® C-Series Human Cancer Discovery Antibody Array 3, RayBiotech) showed that 30 cytokines related to cancer biology were differentially expressed between thyroid normal and tumor tissues. One pair of PTC samples was used to analyze the expression of these cytokines. Four different proteins (Galectin-3, NAG-1/GDF15, TIMP-1, and osteoprotegerin) were selected as biomarker candidates for the diagnosis of thyroid cancer (rectangle). The right graph represents the intensity of each protein. ( B ) Western blot was performed using three pairs of PTC samples to confirm the data of antibody array. The protein expression of galectin-3, mature NAG-1, pro-NAG-1, TIMP-1, and OPG was examined using thyroid tissue samples. The right graph is from the average quantification of protein expression from three patients. N, thyroid normal tissue; T, thyroid tumor tissue. The number of patients is indicated (see Table 1 for details). Uncropped versions of blots presented in Figures S2 and S3 .
    Figure Legend Snippet: Antibody array using human thyroid tissues. ( A ) Antibody array (RayBio ® C-Series Human Cancer Discovery Antibody Array 3, RayBiotech) showed that 30 cytokines related to cancer biology were differentially expressed between thyroid normal and tumor tissues. One pair of PTC samples was used to analyze the expression of these cytokines. Four different proteins (Galectin-3, NAG-1/GDF15, TIMP-1, and osteoprotegerin) were selected as biomarker candidates for the diagnosis of thyroid cancer (rectangle). The right graph represents the intensity of each protein. ( B ) Western blot was performed using three pairs of PTC samples to confirm the data of antibody array. The protein expression of galectin-3, mature NAG-1, pro-NAG-1, TIMP-1, and OPG was examined using thyroid tissue samples. The right graph is from the average quantification of protein expression from three patients. N, thyroid normal tissue; T, thyroid tumor tissue. The number of patients is indicated (see Table 1 for details). Uncropped versions of blots presented in Figures S2 and S3 .

    Techniques Used: Ab Array, Expressing, Biomarker Assay, Western Blot

    3) Product Images from "Total flavonoids of Rhizoma Drynariae combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation"

    Article Title: Total flavonoids of Rhizoma Drynariae combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2021.10050

    Western blot analysis of the expression of bone formation-related proteins in OP rats. (A) Relative expression of RUNX2, OPG and BGP; (B) protein band density was calculated as a ratio relative to β-actin protein levels. The data are expressed as the mean ± SD. Compared with the sham-operated group, * P
    Figure Legend Snippet: Western blot analysis of the expression of bone formation-related proteins in OP rats. (A) Relative expression of RUNX2, OPG and BGP; (B) protein band density was calculated as a ratio relative to β-actin protein levels. The data are expressed as the mean ± SD. Compared with the sham-operated group, * P

    Techniques Used: Western Blot, Expressing

    4) Product Images from "Total flavonoids of Rhizoma drynariae ameliorate steroid-induced avascular necrosis of the femoral head via the PI3K/AKT pathway"

    Article Title: Total flavonoids of Rhizoma drynariae ameliorate steroid-induced avascular necrosis of the femoral head via the PI3K/AKT pathway

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2021.11984

    TFRD increased the expression of VEGF, RUNX2, OPG and OCN and decreased the expression of RANKL in the femoral head of SANFH rats. (A) Protein bands of VEGF, RUNX2, OPG, OCN and RANKL. The expression of (B) VEGF, (C) RUNX2, (D) OPG, (E) OCN and (F) RANKL in the femoral head of rats was detected using western blotting. **P
    Figure Legend Snippet: TFRD increased the expression of VEGF, RUNX2, OPG and OCN and decreased the expression of RANKL in the femoral head of SANFH rats. (A) Protein bands of VEGF, RUNX2, OPG, OCN and RANKL. The expression of (B) VEGF, (C) RUNX2, (D) OPG, (E) OCN and (F) RANKL in the femoral head of rats was detected using western blotting. **P

    Techniques Used: Expressing, Western Blot

    5) Product Images from "Hafnium (IV) oxide obtained by atomic layer deposition (ALD) technology promotes early osteogenesis via activation of Runx2-OPN-mir21A axis while inhibits osteoclasts activity"

    Article Title: Hafnium (IV) oxide obtained by atomic layer deposition (ALD) technology promotes early osteogenesis via activation of Runx2-OPN-mir21A axis while inhibits osteoclasts activity

    Journal: Journal of Nanobiotechnology

    doi: 10.1186/s12951-020-00692-5

    Expression of mRNA, miRNA and cellular localization of proteins involved in osteogenic differentiation in MC3T3 cells cultured on standard glass slides or on the glass slides covered with HfO 2 for 144 h. Relative expression of TGFB , RUNX2 , OPN and OCN was determined using RT-qPCR method ( a ). GAPDH was used as normalization control. Relative expression of osteogenesis-related miRNA ( b ). U6 was used as endogenous control for miRNA qPCR. Additionally, immunufluorescence for RUNX2 ( c ), OPN ( d ) and OPG ( e ) were performed. Error bars represent the means ± SD. * p
    Figure Legend Snippet: Expression of mRNA, miRNA and cellular localization of proteins involved in osteogenic differentiation in MC3T3 cells cultured on standard glass slides or on the glass slides covered with HfO 2 for 144 h. Relative expression of TGFB , RUNX2 , OPN and OCN was determined using RT-qPCR method ( a ). GAPDH was used as normalization control. Relative expression of osteogenesis-related miRNA ( b ). U6 was used as endogenous control for miRNA qPCR. Additionally, immunufluorescence for RUNX2 ( c ), OPN ( d ) and OPG ( e ) were performed. Error bars represent the means ± SD. * p

    Techniques Used: Expressing, Cell Culture, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

    Related Articles

    Incubation:

    Article Title: Total flavonoids of Rhizoma drynariae ameliorate steroid-induced avascular necrosis of the femoral head via the PI3K/AKT pathway
    Article Snippet: .. Thereafter, the membranes were blocked with 5% non-fat milk (Sigma-Aldrich; Merck KGaA) in Tris-buffer saline containing 0.1% Tween-20 for 1 h at room temperature and then incubated with the following primary antibodies overnight at 4°C: Anti-caspase-3 (cat. no. 14220; 1:1,000), anti-Bax (cat. no. 14796; 1:1,000), anti-RUNX2 (cat. no. 12556; 1:1,000), anti-phosphorylated (p-)PI3K (cat. no. 17366; 1:1,000), anti-p-AKT (cat. no. 4060; 1:1,000), anti-AKT (cat. no. 4691; 1:1,000) and anti-glyceraldehyde 3-phosphate dehydrogenase (internal parameter; cat. no. 5174; 1:1,000 all from Cell Signaling Technology, Inc.; anti-Bcl-2 (ab196495; 1:1,000) and anti-OCN (ab13418; 1:1,000), from Abcam; and anti-VEGF (sc-7269; 1:1,000), anti-OPG (sc-390518; 1:1,000), anti-RANKL (sc-377079; 1:1,000) and anti-PI3K (sc-1637; 1:1,000) from Santa Cruz Biotechnology, Inc. ..

    Article Title: Effects of estrogen deficiency during puberty on maxillary and mandibular growth and associated gene expression – an μCT study on rats
    Article Snippet: .. The slides were incubated over night at 4 °C with the primary antibodies diluted in 1% BSA: anti-RANK (polyclonal rabbit antibody H300 sc:9072; diluted 1:25; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), anti-RANKL (polyclonal goat antibody sc:7628; diluted 1:25; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), and anti-OPG (polyclonal goat antibody n-20 sc:8468; diluted 1:25; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA). ..

    Article Title: Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis
    Article Snippet: .. Immunohistochemical analysis Immunolabeling of the histological sections was performed using an indirect immunoperoxidase technique., , Histological sections were arranged into six batches, and each batch was incubated with one of the following primary antibodies (1:100): IL-10 (Rabbit - orb221323, Biorbyt, San Francisco, CA, USA); IL-1β (Rabbit - orb101745 Biorbyt), IL-6 (Rabbit - orb6210 Biorbyt), RANKL (Goat anti-RANKL - SC7627, Santa Cruz Biotechnology), OPG (Rabbit anti-OPG - SC11383, Santa Cruz Biotechnology) and TRAP (Goat anti-TRAP - SC30832, Santa Cruz Biotechnology, Santa Cruz, CA, USA). ..

    Multiple Displacement Amplification:

    Article Title: Total flavonoids of Rhizoma Drynariae combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation
    Article Snippet: .. Experimental reagents CaCO3, TFRD (Guangzhou Baozhilin Pharmacy), NAC (Sigma-Aldrich; Merck KGaA) and kits for superoxide dismutase (SOD; cat. no. A001-3-2), malondialdehyde (MDA; cat. no. A003-1-2), ROS (cat. no. E004-1-1) and glutathione peroxidase (GSH-Px; cat. no. A005-1-2) were purchased from Nanjing Jiancheng Bioengineering Institute; kits for IL-6 (cat. no. ZC-36404), IL-1β (cat. no. ZC-M6681), TNF-α (cat. no. ZC-37624) were purchased from ZCIBIO Technology Co., Ltd.; kits for hematoxylin and eosin (H & E; cat. no. C0105S) staining were purchased from Beyotime Institute of Biotechnology; kits for Masson's staining (cat. no. 60532ES58) were purchased from Shanghai Maikun Chemical Co., Ltd.; kits for protein extraction (cat. no. P1202) and SDS-PAGE gel preparation (cat. no. PG112) were purchased from Shanghai Epizyme Biotech; RIPA buffer reagent (cat. no. P0013) and kit for BCA (cat. no. P0009) were purchased from Beyotime Institute of Biotechnology; primary antibodies against runt-related transcription factor 2 (RUNX2; cat. no. sc-390351), osteoprotegerin (OPG; cat. no. sc-390518), osteocalcin (BGP; cat. no. sc-365797), AKT (cat. no. sc-5298), p-AKT (cat. no. sc-377556), mammalian target of rapamycin (mTOR; cat. no. sc-517464), p-mTOR (cat. no. sc-293133), PI3K (cat. no. sc-365290) and p-PI3K (cat. no. sc-1637) were purchased from Santa Cruz Biotechnology, Inc. Horseradish peroxidase-labeled goat anti-rabbit secondary antibody (cat. no. #56970) was purchased from Cell Signaling Technology, Inc. kit for Enhanced chemiluminescence (ECL; cat. no. KF001) was purchased from Affinity Biosciences. ..

    Staining:

    Article Title: Total flavonoids of Rhizoma Drynariae combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation
    Article Snippet: .. Experimental reagents CaCO3, TFRD (Guangzhou Baozhilin Pharmacy), NAC (Sigma-Aldrich; Merck KGaA) and kits for superoxide dismutase (SOD; cat. no. A001-3-2), malondialdehyde (MDA; cat. no. A003-1-2), ROS (cat. no. E004-1-1) and glutathione peroxidase (GSH-Px; cat. no. A005-1-2) were purchased from Nanjing Jiancheng Bioengineering Institute; kits for IL-6 (cat. no. ZC-36404), IL-1β (cat. no. ZC-M6681), TNF-α (cat. no. ZC-37624) were purchased from ZCIBIO Technology Co., Ltd.; kits for hematoxylin and eosin (H & E; cat. no. C0105S) staining were purchased from Beyotime Institute of Biotechnology; kits for Masson's staining (cat. no. 60532ES58) were purchased from Shanghai Maikun Chemical Co., Ltd.; kits for protein extraction (cat. no. P1202) and SDS-PAGE gel preparation (cat. no. PG112) were purchased from Shanghai Epizyme Biotech; RIPA buffer reagent (cat. no. P0013) and kit for BCA (cat. no. P0009) were purchased from Beyotime Institute of Biotechnology; primary antibodies against runt-related transcription factor 2 (RUNX2; cat. no. sc-390351), osteoprotegerin (OPG; cat. no. sc-390518), osteocalcin (BGP; cat. no. sc-365797), AKT (cat. no. sc-5298), p-AKT (cat. no. sc-377556), mammalian target of rapamycin (mTOR; cat. no. sc-517464), p-mTOR (cat. no. sc-293133), PI3K (cat. no. sc-365290) and p-PI3K (cat. no. sc-1637) were purchased from Santa Cruz Biotechnology, Inc. Horseradish peroxidase-labeled goat anti-rabbit secondary antibody (cat. no. #56970) was purchased from Cell Signaling Technology, Inc. kit for Enhanced chemiluminescence (ECL; cat. no. KF001) was purchased from Affinity Biosciences. ..

    Protein Extraction:

    Article Title: Total flavonoids of Rhizoma Drynariae combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation
    Article Snippet: .. Experimental reagents CaCO3, TFRD (Guangzhou Baozhilin Pharmacy), NAC (Sigma-Aldrich; Merck KGaA) and kits for superoxide dismutase (SOD; cat. no. A001-3-2), malondialdehyde (MDA; cat. no. A003-1-2), ROS (cat. no. E004-1-1) and glutathione peroxidase (GSH-Px; cat. no. A005-1-2) were purchased from Nanjing Jiancheng Bioengineering Institute; kits for IL-6 (cat. no. ZC-36404), IL-1β (cat. no. ZC-M6681), TNF-α (cat. no. ZC-37624) were purchased from ZCIBIO Technology Co., Ltd.; kits for hematoxylin and eosin (H & E; cat. no. C0105S) staining were purchased from Beyotime Institute of Biotechnology; kits for Masson's staining (cat. no. 60532ES58) were purchased from Shanghai Maikun Chemical Co., Ltd.; kits for protein extraction (cat. no. P1202) and SDS-PAGE gel preparation (cat. no. PG112) were purchased from Shanghai Epizyme Biotech; RIPA buffer reagent (cat. no. P0013) and kit for BCA (cat. no. P0009) were purchased from Beyotime Institute of Biotechnology; primary antibodies against runt-related transcription factor 2 (RUNX2; cat. no. sc-390351), osteoprotegerin (OPG; cat. no. sc-390518), osteocalcin (BGP; cat. no. sc-365797), AKT (cat. no. sc-5298), p-AKT (cat. no. sc-377556), mammalian target of rapamycin (mTOR; cat. no. sc-517464), p-mTOR (cat. no. sc-293133), PI3K (cat. no. sc-365290) and p-PI3K (cat. no. sc-1637) were purchased from Santa Cruz Biotechnology, Inc. Horseradish peroxidase-labeled goat anti-rabbit secondary antibody (cat. no. #56970) was purchased from Cell Signaling Technology, Inc. kit for Enhanced chemiluminescence (ECL; cat. no. KF001) was purchased from Affinity Biosciences. ..

    SDS Page:

    Article Title: Total flavonoids of Rhizoma Drynariae combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation
    Article Snippet: .. Experimental reagents CaCO3, TFRD (Guangzhou Baozhilin Pharmacy), NAC (Sigma-Aldrich; Merck KGaA) and kits for superoxide dismutase (SOD; cat. no. A001-3-2), malondialdehyde (MDA; cat. no. A003-1-2), ROS (cat. no. E004-1-1) and glutathione peroxidase (GSH-Px; cat. no. A005-1-2) were purchased from Nanjing Jiancheng Bioengineering Institute; kits for IL-6 (cat. no. ZC-36404), IL-1β (cat. no. ZC-M6681), TNF-α (cat. no. ZC-37624) were purchased from ZCIBIO Technology Co., Ltd.; kits for hematoxylin and eosin (H & E; cat. no. C0105S) staining were purchased from Beyotime Institute of Biotechnology; kits for Masson's staining (cat. no. 60532ES58) were purchased from Shanghai Maikun Chemical Co., Ltd.; kits for protein extraction (cat. no. P1202) and SDS-PAGE gel preparation (cat. no. PG112) were purchased from Shanghai Epizyme Biotech; RIPA buffer reagent (cat. no. P0013) and kit for BCA (cat. no. P0009) were purchased from Beyotime Institute of Biotechnology; primary antibodies against runt-related transcription factor 2 (RUNX2; cat. no. sc-390351), osteoprotegerin (OPG; cat. no. sc-390518), osteocalcin (BGP; cat. no. sc-365797), AKT (cat. no. sc-5298), p-AKT (cat. no. sc-377556), mammalian target of rapamycin (mTOR; cat. no. sc-517464), p-mTOR (cat. no. sc-293133), PI3K (cat. no. sc-365290) and p-PI3K (cat. no. sc-1637) were purchased from Santa Cruz Biotechnology, Inc. Horseradish peroxidase-labeled goat anti-rabbit secondary antibody (cat. no. #56970) was purchased from Cell Signaling Technology, Inc. kit for Enhanced chemiluminescence (ECL; cat. no. KF001) was purchased from Affinity Biosciences. ..

    Immunohistochemistry:

    Article Title: Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis
    Article Snippet: .. Immunohistochemical analysis Immunolabeling of the histological sections was performed using an indirect immunoperoxidase technique., , Histological sections were arranged into six batches, and each batch was incubated with one of the following primary antibodies (1:100): IL-10 (Rabbit - orb221323, Biorbyt, San Francisco, CA, USA); IL-1β (Rabbit - orb101745 Biorbyt), IL-6 (Rabbit - orb6210 Biorbyt), RANKL (Goat anti-RANKL - SC7627, Santa Cruz Biotechnology), OPG (Rabbit anti-OPG - SC11383, Santa Cruz Biotechnology) and TRAP (Goat anti-TRAP - SC30832, Santa Cruz Biotechnology, Santa Cruz, CA, USA). ..

    Immunolabeling:

    Article Title: Dietary supplementation with multi-strain formula of probiotics modulates inflammatory and immunological markers in apical periodontitis
    Article Snippet: .. Immunohistochemical analysis Immunolabeling of the histological sections was performed using an indirect immunoperoxidase technique., , Histological sections were arranged into six batches, and each batch was incubated with one of the following primary antibodies (1:100): IL-10 (Rabbit - orb221323, Biorbyt, San Francisco, CA, USA); IL-1β (Rabbit - orb101745 Biorbyt), IL-6 (Rabbit - orb6210 Biorbyt), RANKL (Goat anti-RANKL - SC7627, Santa Cruz Biotechnology), OPG (Rabbit anti-OPG - SC11383, Santa Cruz Biotechnology) and TRAP (Goat anti-TRAP - SC30832, Santa Cruz Biotechnology, Santa Cruz, CA, USA). ..

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  • 98
    Santa Cruz Biotechnology anti osteoprotegrin opg
    Induction of RANKL expression by TiPs. (A) The gene expression of RANKL and <t>OPG</t> in TiPs-treated fibroblasts were examined by real-time PCR. (B) Western blots analysis of RANKL and OPG in fibroblasts following treatment with TiPs for various time periods. (C) The density of western blots bands shown in (B) was quantified using the ImageJ software. (D and E) The expression of sRANKL in the supernatants from each group were quantified by ELISA. Data are represented as the means ± S.E.M from three independent experiments. (A and C) *P
    Anti Osteoprotegrin Opg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti osteoprotegrin opg/product/Santa Cruz Biotechnology
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti osteoprotegrin opg - by Bioz Stars, 2021-09
    98/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology anti opg
    Expression of receptor activator of nuclear factor κB ligand (RANKL) and osteoprotegerin <t>(OPG)</t> mRNA in alveolar bone cells (ABCs) and dental pulp cells (DPCs). ( A ) Phase-contrast image of ABCs isolated from green fluorescent protein (GFP) rats
    Anti Opg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti opg/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti opg - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    Induction of RANKL expression by TiPs. (A) The gene expression of RANKL and OPG in TiPs-treated fibroblasts were examined by real-time PCR. (B) Western blots analysis of RANKL and OPG in fibroblasts following treatment with TiPs for various time periods. (C) The density of western blots bands shown in (B) was quantified using the ImageJ software. (D and E) The expression of sRANKL in the supernatants from each group were quantified by ELISA. Data are represented as the means ± S.E.M from three independent experiments. (A and C) *P

    Journal: PLoS ONE

    Article Title: ER Stress Mediates TiAl6V4 Particle-Induced Peri-Implant Osteolysis by Promoting RANKL Expression in Fibroblasts

    doi: 10.1371/journal.pone.0137774

    Figure Lengend Snippet: Induction of RANKL expression by TiPs. (A) The gene expression of RANKL and OPG in TiPs-treated fibroblasts were examined by real-time PCR. (B) Western blots analysis of RANKL and OPG in fibroblasts following treatment with TiPs for various time periods. (C) The density of western blots bands shown in (B) was quantified using the ImageJ software. (D and E) The expression of sRANKL in the supernatants from each group were quantified by ELISA. Data are represented as the means ± S.E.M from three independent experiments. (A and C) *P

    Article Snippet: Western blot was performed using the following primary antibodies: anti-inositol-requiring kinase 1 (IRE1-a), anti-glucose-regulated protein 78 (GRP/Bip), anti-C/EBP homologous protein (CHOP), and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Cell Signaling Technology, Beverly, MA, USA); anti-receptor activator of nuclear factor kappa-B ligand (RANKL) and anti- osteoprotegrin (OPG) (Santa Cruz, CA, USA); subsequently, the following secondary antibodies were applied: horseradish peroxidase-conjugated anti-rabbit IgG (Cell Signaling Technology) and horseradish peroxidase-conjugated anti-mouse IgG (Santa Cruz, CA, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Software, Enzyme-linked Immunosorbent Assay

    ER stress mediated the upregulation of RANKL and the RANKL/OPG ratio in TiPs-stimulated fibroblasts. (A and B) The gene expression of RANKL and OPG in fibroblasts from each group were examined by real-time PCR. (C, E and G) Western blots analysis of RANKL and OPG from each group. (D, F and H) The density of western blots bands shown in (C, E and G) was quantified using the ImageJ software. (I) The expression of sRANKL in the supernatants of fibroblasts from each group were quantified by ELISA. Data are represented as the means ± S.E.M from three independent experiments. *P

    Journal: PLoS ONE

    Article Title: ER Stress Mediates TiAl6V4 Particle-Induced Peri-Implant Osteolysis by Promoting RANKL Expression in Fibroblasts

    doi: 10.1371/journal.pone.0137774

    Figure Lengend Snippet: ER stress mediated the upregulation of RANKL and the RANKL/OPG ratio in TiPs-stimulated fibroblasts. (A and B) The gene expression of RANKL and OPG in fibroblasts from each group were examined by real-time PCR. (C, E and G) Western blots analysis of RANKL and OPG from each group. (D, F and H) The density of western blots bands shown in (C, E and G) was quantified using the ImageJ software. (I) The expression of sRANKL in the supernatants of fibroblasts from each group were quantified by ELISA. Data are represented as the means ± S.E.M from three independent experiments. *P

    Article Snippet: Western blot was performed using the following primary antibodies: anti-inositol-requiring kinase 1 (IRE1-a), anti-glucose-regulated protein 78 (GRP/Bip), anti-C/EBP homologous protein (CHOP), and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Cell Signaling Technology, Beverly, MA, USA); anti-receptor activator of nuclear factor kappa-B ligand (RANKL) and anti- osteoprotegrin (OPG) (Santa Cruz, CA, USA); subsequently, the following secondary antibodies were applied: horseradish peroxidase-conjugated anti-rabbit IgG (Cell Signaling Technology) and horseradish peroxidase-conjugated anti-mouse IgG (Santa Cruz, CA, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Software, Enzyme-linked Immunosorbent Assay

    ABE regulates the expression of RANKL and OPG in bone marrow mesenchymal stem cells (BMSCs). BMSCs were cultured with or without different concentrations of ABE (0.16, 0.8, 4 μg/ml, respectively). Three days post-culture, supernatants were obtained to detectthe amounts of RANKL (A) and OPG (B) in the supernatants by ELISA. (C) refers to the ratio of RANKL/OPG in the supernatants. Data are represented as the mean ± SD of three independent experiments. *P

    Journal: Journal of Translational Medicine

    Article Title: Achyranthes bidentata extract exerts osteoprotective effects on steroid-induced osteonecrosis of the femoral head in rats by regulating RANKL/RANK/OPG signaling

    doi: 10.1186/s12967-014-0334-7

    Figure Lengend Snippet: ABE regulates the expression of RANKL and OPG in bone marrow mesenchymal stem cells (BMSCs). BMSCs were cultured with or without different concentrations of ABE (0.16, 0.8, 4 μg/ml, respectively). Three days post-culture, supernatants were obtained to detectthe amounts of RANKL (A) and OPG (B) in the supernatants by ELISA. (C) refers to the ratio of RANKL/OPG in the supernatants. Data are represented as the mean ± SD of three independent experiments. *P

    Article Snippet: The following antibodies were used: RANK antibody (rabbit antibody, dilution 1:50, Cell Signaling Technology, Inc., Danvers, MA, USA), RANKL antibody (rabbit antibody, dilution 1:100, Millipore Corporation, Billerica, MA, USA), OPG antibody (rabbit antibody, dilution 1:100, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), and GAPDH antibody (internal control, rabbit polyclonal antibody, dilution 1:200, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA).

    Techniques: Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay

    ABE treatment regulates the RANKL/RANK/OPG signaling pathway in rats with steroid-induced ONFH. RANK, RANKL, and OPG levels in the serum of rats with steroid-induced ONFH with or without ABE treatment were detected by ELISA (A) . RANKL, RANK, and OPG expression in the femoral heads of rats with steroid-induced ONFH with or without ABE treatment were detected at mRNA and protein levels by quantitative real-time RT-PCR (B) , western blot (C) , respectively. (D) shows the ratio of RANKL/OPG in the serum, the ratio of RANKL mRNA/OPG mRNA, and the ratio of RANK protein/OPG protein in the rats with steroid-induced ONFH with and without ABE treatment. Data are presented as the mean ± S.D. (n =20 for control, n =25 for model, n =20 for ABE treatment groups). ##: P

    Journal: Journal of Translational Medicine

    Article Title: Achyranthes bidentata extract exerts osteoprotective effects on steroid-induced osteonecrosis of the femoral head in rats by regulating RANKL/RANK/OPG signaling

    doi: 10.1186/s12967-014-0334-7

    Figure Lengend Snippet: ABE treatment regulates the RANKL/RANK/OPG signaling pathway in rats with steroid-induced ONFH. RANK, RANKL, and OPG levels in the serum of rats with steroid-induced ONFH with or without ABE treatment were detected by ELISA (A) . RANKL, RANK, and OPG expression in the femoral heads of rats with steroid-induced ONFH with or without ABE treatment were detected at mRNA and protein levels by quantitative real-time RT-PCR (B) , western blot (C) , respectively. (D) shows the ratio of RANKL/OPG in the serum, the ratio of RANKL mRNA/OPG mRNA, and the ratio of RANK protein/OPG protein in the rats with steroid-induced ONFH with and without ABE treatment. Data are presented as the mean ± S.D. (n =20 for control, n =25 for model, n =20 for ABE treatment groups). ##: P

    Article Snippet: The following antibodies were used: RANK antibody (rabbit antibody, dilution 1:50, Cell Signaling Technology, Inc., Danvers, MA, USA), RANKL antibody (rabbit antibody, dilution 1:100, Millipore Corporation, Billerica, MA, USA), OPG antibody (rabbit antibody, dilution 1:100, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), and GAPDH antibody (internal control, rabbit polyclonal antibody, dilution 1:200, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA).

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Western Blot

    Effects of DKK2 on hFOB1.19 cell proliferation and osteogenic differentiation. (A) DKK2 knockdown was generated in hFOB1.19 cells by transfection with si-DKK2, as confirmed by RT-qPCR. hFOB1.19 cells were transfected with si-DKK2 and examined for (B) cell viability by CCK-8 assay; (C) DNA synthesis capacity by EdU assay, and (D) cell apoptosis by flow cytometric assay. (E) The protein levels of Ki-67, cleaved-caspase-3, caspase-3, WNT1, β-catenin, cyclin D1, RANKL and OPG were determined by western blot analysis, and (F) the mRNA expression of osteogenesis markers, including ALP, RUNX2, OCN and OPN, was determined by RT-qPCR. (G) The mineralization of hFOB1.19 cells was determined by Alizarin Red staining. * P

    Journal: International Journal of Molecular Medicine

    Article Title: miR-483-3p promotes the osteogenesis of human osteoblasts by targeting Dikkopf 2 (DKK2) and the Wnt signaling pathway

    doi: 10.3892/ijmm.2020.4694

    Figure Lengend Snippet: Effects of DKK2 on hFOB1.19 cell proliferation and osteogenic differentiation. (A) DKK2 knockdown was generated in hFOB1.19 cells by transfection with si-DKK2, as confirmed by RT-qPCR. hFOB1.19 cells were transfected with si-DKK2 and examined for (B) cell viability by CCK-8 assay; (C) DNA synthesis capacity by EdU assay, and (D) cell apoptosis by flow cytometric assay. (E) The protein levels of Ki-67, cleaved-caspase-3, caspase-3, WNT1, β-catenin, cyclin D1, RANKL and OPG were determined by western blot analysis, and (F) the mRNA expression of osteogenesis markers, including ALP, RUNX2, OCN and OPN, was determined by RT-qPCR. (G) The mineralization of hFOB1.19 cells was determined by Alizarin Red staining. * P

    Article Snippet: The membranes were blocked for 2 h at 37°C with 5% non-fat milk in Tris-buffered saline with Tween-20 (TBST) and then incubated overnight at 4°C with the following primary antibodies: Ki-67 (dilution 1:1,000, 27309-1-AP; Wuhan Sanying Biotechnology), cleaved-caspase-3 (dilution 1:1,000, ab2302; Abcam), caspase-3 (dilution 1:1,000, 19677-1-AP; Wuhan Sanying Biotechnology), WNT1 (dilution 1:1,000, 27935-1-AP; Wuhan Sanying Biotechnology), β-catenin (dilution 1:1,000, 51067-2-AP; Wuhan Sanying Biotechnology), cyclin D1 (dilution 1:1,000, 60186-1-Ig; Wuhan Sanying Biotechnology), DKK2 (dilution 1:1,000, CSB-PA08279A0Rb; CUSABIO), RANKL (dilution 1:1,000, sc-9073, Santa Cruz Biotechnology, Inc.), OPG (dilution 1:1,000, sc-11383, Santa Cruz Biotechnology, Inc.) and GAPDH (dilution 1:3,000, T0004; Affinity Biosciences).

    Techniques: Generated, Transfection, Quantitative RT-PCR, CCK-8 Assay, DNA Synthesis, EdU Assay, Flow Cytometry, Western Blot, Expressing, Staining

    Effects of miR-483-3p on hFOB1.19 cell proliferation and osteogenic differentiation. (A) miR-483-3p overexpression was generated in hFOB1.19 cells by transfection with miR-483-3p, as confirmed by RT-qPCR. hFOB1.19 cells were transfected with miR-483-3p and examined for (B) cell viability by CCK-8 assay; (C) DNA synthesis capacity by EdU assay, and (D) cell apoptosis by flow cytometric assay. (E) The protein levels of Ki-67, cleaved-caspase-3, caspase-3, WNT1, β-catenin, cyclin D1, RANKL and OPG were examined by western blot analysis, and (F) the mRNA expression of osteogenesis markers, including ALP, RUNX2, OCN and OPN was determined by RT-qPCR. (G) The mineralization of hFOB1.19 cells was determined by Alizarin Red staining. * P

    Journal: International Journal of Molecular Medicine

    Article Title: miR-483-3p promotes the osteogenesis of human osteoblasts by targeting Dikkopf 2 (DKK2) and the Wnt signaling pathway

    doi: 10.3892/ijmm.2020.4694

    Figure Lengend Snippet: Effects of miR-483-3p on hFOB1.19 cell proliferation and osteogenic differentiation. (A) miR-483-3p overexpression was generated in hFOB1.19 cells by transfection with miR-483-3p, as confirmed by RT-qPCR. hFOB1.19 cells were transfected with miR-483-3p and examined for (B) cell viability by CCK-8 assay; (C) DNA synthesis capacity by EdU assay, and (D) cell apoptosis by flow cytometric assay. (E) The protein levels of Ki-67, cleaved-caspase-3, caspase-3, WNT1, β-catenin, cyclin D1, RANKL and OPG were examined by western blot analysis, and (F) the mRNA expression of osteogenesis markers, including ALP, RUNX2, OCN and OPN was determined by RT-qPCR. (G) The mineralization of hFOB1.19 cells was determined by Alizarin Red staining. * P

    Article Snippet: The membranes were blocked for 2 h at 37°C with 5% non-fat milk in Tris-buffered saline with Tween-20 (TBST) and then incubated overnight at 4°C with the following primary antibodies: Ki-67 (dilution 1:1,000, 27309-1-AP; Wuhan Sanying Biotechnology), cleaved-caspase-3 (dilution 1:1,000, ab2302; Abcam), caspase-3 (dilution 1:1,000, 19677-1-AP; Wuhan Sanying Biotechnology), WNT1 (dilution 1:1,000, 27935-1-AP; Wuhan Sanying Biotechnology), β-catenin (dilution 1:1,000, 51067-2-AP; Wuhan Sanying Biotechnology), cyclin D1 (dilution 1:1,000, 60186-1-Ig; Wuhan Sanying Biotechnology), DKK2 (dilution 1:1,000, CSB-PA08279A0Rb; CUSABIO), RANKL (dilution 1:1,000, sc-9073, Santa Cruz Biotechnology, Inc.), OPG (dilution 1:1,000, sc-11383, Santa Cruz Biotechnology, Inc.) and GAPDH (dilution 1:3,000, T0004; Affinity Biosciences).

    Techniques: Over Expression, Generated, Transfection, Quantitative RT-PCR, CCK-8 Assay, DNA Synthesis, EdU Assay, Flow Cytometry, Western Blot, Expressing, Staining

    Dynamic effects of miR-483-3p and DKK2 on hFOB1.19 cell proliferation and osteogenic differentiation of hFOB1.19 cells co-transfected with miR-483-3p and DKK2 OE and examined for (A) cell viability by CCK-8 assay; (B) DNA synthesis capacity by EdU assay and (C) cell apoptosis by flow cytometry assay. (D) The protein levels of Ki-67, cleaved-caspase-3, caspase-3, DKK2, WNT1, β-catenin, cyclin D1, RANKL and OPG were detected by western blot analysis, and (E) the mRNA expression of osteogenesis markers, including ALP, RUNX2, OCN and OPN were determined by RT-qPCR. (F) The mineralization of hFOB1.19 cells was determined by Alizarin Red staining. * P

    Journal: International Journal of Molecular Medicine

    Article Title: miR-483-3p promotes the osteogenesis of human osteoblasts by targeting Dikkopf 2 (DKK2) and the Wnt signaling pathway

    doi: 10.3892/ijmm.2020.4694

    Figure Lengend Snippet: Dynamic effects of miR-483-3p and DKK2 on hFOB1.19 cell proliferation and osteogenic differentiation of hFOB1.19 cells co-transfected with miR-483-3p and DKK2 OE and examined for (A) cell viability by CCK-8 assay; (B) DNA synthesis capacity by EdU assay and (C) cell apoptosis by flow cytometry assay. (D) The protein levels of Ki-67, cleaved-caspase-3, caspase-3, DKK2, WNT1, β-catenin, cyclin D1, RANKL and OPG were detected by western blot analysis, and (E) the mRNA expression of osteogenesis markers, including ALP, RUNX2, OCN and OPN were determined by RT-qPCR. (F) The mineralization of hFOB1.19 cells was determined by Alizarin Red staining. * P

    Article Snippet: The membranes were blocked for 2 h at 37°C with 5% non-fat milk in Tris-buffered saline with Tween-20 (TBST) and then incubated overnight at 4°C with the following primary antibodies: Ki-67 (dilution 1:1,000, 27309-1-AP; Wuhan Sanying Biotechnology), cleaved-caspase-3 (dilution 1:1,000, ab2302; Abcam), caspase-3 (dilution 1:1,000, 19677-1-AP; Wuhan Sanying Biotechnology), WNT1 (dilution 1:1,000, 27935-1-AP; Wuhan Sanying Biotechnology), β-catenin (dilution 1:1,000, 51067-2-AP; Wuhan Sanying Biotechnology), cyclin D1 (dilution 1:1,000, 60186-1-Ig; Wuhan Sanying Biotechnology), DKK2 (dilution 1:1,000, CSB-PA08279A0Rb; CUSABIO), RANKL (dilution 1:1,000, sc-9073, Santa Cruz Biotechnology, Inc.), OPG (dilution 1:1,000, sc-11383, Santa Cruz Biotechnology, Inc.) and GAPDH (dilution 1:3,000, T0004; Affinity Biosciences).

    Techniques: Transfection, CCK-8 Assay, DNA Synthesis, EdU Assay, Flow Cytometry, Western Blot, Expressing, Quantitative RT-PCR, Staining

    Expression of receptor activator of nuclear factor κB ligand (RANKL) and osteoprotegerin (OPG) mRNA in alveolar bone cells (ABCs) and dental pulp cells (DPCs). ( A ) Phase-contrast image of ABCs isolated from green fluorescent protein (GFP) rats

    Journal: Journal of Dental Research

    Article Title: Mesenchymal Dental Pulp Cells Attenuate Dentin Resorption in Homeostasis

    doi: 10.1177/0022034515575347

    Figure Lengend Snippet: Expression of receptor activator of nuclear factor κB ligand (RANKL) and osteoprotegerin (OPG) mRNA in alveolar bone cells (ABCs) and dental pulp cells (DPCs). ( A ) Phase-contrast image of ABCs isolated from green fluorescent protein (GFP) rats

    Article Snippet: The antibodies were goat anti-RANKL polyclonal antibody and anti-OPG a polyclonal antibody (sc-7628 and sc-8468; 1:200 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA).

    Techniques: Expressing, Isolation