Article Title: Akaby - cell-free protein expression system for linear templates
Figure Lengend Snippet: PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.
Article Snippet: The quantitative PCR reaction mix was prepared by mixing 2 μl of complementary DNA from the reverse transcription with 2 μl of 10 μM forward and reverse primers, 11.25 μl OneTaq Hot Start 2X Master Mix with Standard Buffer (Catalog No. M0484, New England BioLabs Inc.), 1.25 μl Chai Green Dye 20X (Catalog No. R01200, Chai Bio), and 7.5 μl of nuclease-free water.
Techniques: Polymerase Chain Reaction, Amplification, Construct, Plasmid Preparation, Sequencing