onetaq quick load 2x master mix with standard buffer  (New England Biolabs)


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    New England Biolabs onetaq quick load 2x master mix with standard buffer
    PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.
    Onetaq Quick Load 2x Master Mix With Standard Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/onetaq quick load 2x master mix with standard buffer/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    onetaq quick load 2x master mix with standard buffer - by Bioz Stars, 2022-07
    96/100 stars

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    1) Product Images from "Akaby - cell-free protein expression system for linear templates"

    Article Title: Akaby - cell-free protein expression system for linear templates

    Journal: bioRxiv

    doi: 10.1101/2021.11.03.467179

    PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.
    Figure Legend Snippet: PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.

    Techniques Used: Polymerase Chain Reaction, Amplification, Construct, Plasmid Preparation, Sequencing

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    New England Biolabs onetaq quick load 2x master mix with standard buffer
    PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.
    Onetaq Quick Load 2x Master Mix With Standard Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/onetaq quick load 2x master mix with standard buffer/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    onetaq quick load 2x master mix with standard buffer - by Bioz Stars, 2022-07
    96/100 stars
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    PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.

    Journal: bioRxiv

    Article Title: Akaby - cell-free protein expression system for linear templates

    doi: 10.1101/2021.11.03.467179

    Figure Lengend Snippet: PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.

    Article Snippet: 10 μl total volume of colony PCR was performed with 0.4 μM of locus-(Primer 1 and 4) and insert-(Primer 2 and 3) specific primer pairs, 1X OneTaq® Quick-Load® 2X Master Mix with Standard Buffer (New England BioLabs Inc., M0486L), and 2 μl of water suspended colony.

    Techniques: Polymerase Chain Reaction, Amplification, Construct, Plasmid Preparation, Sequencing