onetaq hot start 2x master mix  (New England Biolabs)


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    New England Biolabs onetaq hot start 2x master mix
    PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.
    Onetaq Hot Start 2x Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/onetaq hot start 2x master mix/product/New England Biolabs
    Average 95 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    onetaq hot start 2x master mix - by Bioz Stars, 2022-08
    95/100 stars

    Images

    1) Product Images from "Akaby - cell-free protein expression system for linear templates"

    Article Title: Akaby - cell-free protein expression system for linear templates

    Journal: bioRxiv

    doi: 10.1101/2021.11.03.467179

    PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.
    Figure Legend Snippet: PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.

    Techniques Used: Polymerase Chain Reaction, Amplification, Construct, Plasmid Preparation, Sequencing

    2) Product Images from "A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay"

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-40035-5

    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.
    Figure Legend Snippet: Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Techniques Used: Polymerase Chain Reaction, Hot Start PCR

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    New England Biolabs onetaq hot start 2x master mix
    PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.
    Onetaq Hot Start 2x Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/onetaq hot start 2x master mix/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    onetaq hot start 2x master mix - by Bioz Stars, 2022-08
    95/100 stars
      Buy from Supplier

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    PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.

    Journal: bioRxiv

    Article Title: Akaby - cell-free protein expression system for linear templates

    doi: 10.1101/2021.11.03.467179

    Figure Lengend Snippet: PCR amplified eGFP gene construct. The design of a 898 nt length DNA oligo. The oligo was PCR amplified from T7Max-GFP plasmid with Primer 11 and Primer 12 with LongAmp Taq 2X Master Mix (New England BioLabs Inc., M0287S). T7Max, the enhanced T7 polymerase promoter sequence; eGFP gene, an eGFP coding sequence; His, a 8xHistidine taq sequence; T500, a transcription terminator sequence.

    Article Snippet: The quantitative PCR reaction mix was prepared by mixing 2 μl of complementary DNA from the reverse transcription with 2 μl of 10 μM forward and reverse primers, 11.25 μl OneTaq Hot Start 2X Master Mix with Standard Buffer (Catalog No. M0484, New England BioLabs Inc.), 1.25 μl Chai Green Dye 20X (Catalog No. R01200, Chai Bio), and 7.5 μl of nuclease-free water.

    Techniques: Polymerase Chain Reaction, Amplification, Construct, Plasmid Preparation, Sequencing

    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Journal: Scientific Reports

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay

    doi: 10.1038/s41598-019-40035-5

    Figure Lengend Snippet: Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Article Snippet: Several master mixes were tested in the optimization experiments, namely AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA), AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA), OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA), Platinum Hot Start PCR Master Mix (Invitrogen, California, USA), and HotStarTaq DNA Polymerase (Qiagen, Germany).

    Techniques: Polymerase Chain Reaction, Hot Start PCR