Journal: bioRxiv

Article Title: Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and Identification of Potential Bacterial Chassis for studying Plant-Microbe Interactions

doi: 10.1101/2023.06.05.543168

Figure Lengend Snippet: An overview of the MRTK cloning process, parts within the toolkit, origin compatibility, origin copy number and antimicrobial susceptibility for each strain. A) Cloning plasmids (C plasmids) are used as starting material with BsaI restriction enzyme and NEBridge MasterMix to clone the product plasmid (P plasmid). P plasmids can then be transformed into the desired SynCom strain of interest with a time of ∼2.5 hours from plasmid to plate. B) Overview of all 45 parts included in the MRTK and their 4 bp golden gate overhang sequences. C) A table showing the compatibility and copy number of the origins of the MRTK for each strain as well as their antimicrobial susceptibility. Green boxes indicate that the origin is replicated in the strain and that the strain is susceptible to the antimicrobial agent. Red boxes indicate that the origin is not replicated in the strain and that the strain will grow on that antimicrobial agent.

Article Snippet: Colony PCR reactions for sequence verification were performed with NEB OneTaq HotStart 2x MasterMix (M0484S) and PCR reactions for molecular cloning were performed with Q5 HotStart 2x MasterMix (M0494S).

Techniques: Clone Assay, Plasmid Preparation, Transformation Assay

Journal: Journal of visualized experiments : JoVE

Article Title: Establishment and Maintenance of Gnotobiotic American Cockroaches ( Periplaneta americana )

doi: 10.3791/61316

Figure Lengend Snippet: 6. Quality control of sterility

Article Snippet: Extract DNA from nymph homogenate using a bacterial DNA extraction kit ( ) as follows: table ft1 table-wrap mode="anchored" t5 Name of Material/ Equipment Company Catalog Number Comments/Description 2X master mix New England BioLabs M0482 OneTaq MasterMix Autoclavable rat chow Zeigler NIH-31 Modified Auto Bacterial DNA extraction kit Omega Bio-Tek D-3350 E.Z.N.A.

Techniques: Modification, DNA Extraction, Binding Assay, DNA Purification, Hybridization, Spectrophotometry