Journal: BioTech
Article Title: Strategies for Increasing the Throughput of Genetic Screening: Lessons Learned from the COVID-19 Pandemic within a University Community
doi: 10.3390/biotech13030026
Figure Lengend Snippet: Workflow for COVID-19 mass screening using RT-qPCR-based diagnosis and relying on the developed strategies of low-risk sample pooling and multiplex analysis for predictably high-viral-load samples. In the orange box, numbers 1 and 2 represent the start of the procedure of triage and risk assessment of individuals. The pathways for Yes and No indicate the approach to be followed. Developed methodologies described in this work are in green boxes, and standard procedures are in light blue boxes. An RT-qPCR was considered dubious when at least one viral target was amplified and/or had Cp values above the standardized 35-cycle threshold. This workflow was created using BioRender.com and PowerPoint.
Article Snippet: RT-qPCR analysis was performed using NZYSpeedy One-step RT-qPCR Probe Master Mix (NZYTech, Lisbon, Portugal).
Techniques: Quantitative RT-PCR, Multiplex Assay, Amplification