on column dnasei digestion  (Qiagen)


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    Structured Review

    Qiagen on column dnasei digestion
    On Column Dnasei Digestion, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/on column dnasei digestion/product/Qiagen
    Average 97 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    on column dnasei digestion - by Bioz Stars, 2019-12
    97/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Mutation of the cytosolic ribosomal protein-encoding RPS10B gene affects shoot meristematic function in Arabidopsis
    Article Snippet: Paragraph title: RNA isolation, RT-PCR, cloning ... Total RNA was extracted using the RNeasy plant miniprep kit with on-column DNaseI digestion (Qiagen, Hilden, Germany) from about 100 mg tissue powder, obtained from 10 pooled 1-cm primary inflorescence stem base segments per genotype, from bolting plants of about 25 cm height. cDNA synthesis was performed from 1 μg total RNA in a total volume of 10 μl with SuperscriptII (Invitrogen, Life Technologies, Carlsbad, CA) and oligo-dT primer.

    Article Title: A common variant in the 3?UTR of the GRIK4 glutamate receptor gene affects transcript abundance and protects against bipolar disorder
    Article Snippet: The cloned GRIK4 sequences compose the majority of the final coding exon sequence and ≈1 kb of 3′UTR. .. Cells were cultured for another 24 h and then harvested using trypsin/versene in preparation for total RNA extraction using a RNeasy Mini Kit and on-column DNaseI digestion (Qiagen).

    Amplification:

    Article Title: The Formation and Sequestration of Nonendogenous Ketocarotenoids in Transgenic Nicotiana glauca
    Article Snippet: RNA was extracted using the RNeasy reagents and protocol, including on-column DNaseI digestion (Qiagen). .. The Rotor-Gene SYBR Green RT-PCR Kit was used to determine gene expression levels, and the Rotor-Gene SYBR Green PCR Kit (Qiagen) was used to determine transgene copy number.

    Article Title: Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development
    Article Snippet: Total RNA was extracted using PicoPure RNA isolation Kit (Life Technologies, CA) or AllPrep DNA/RNA Micro Kit (Qiagen, Germany) with an on-column DNaseI digestion (Qiagen, Germany). cDNA was prepared using SuperScript III (Life Technologies, CA) and random hexamer primers (Life Technologies, CA). .. Total RNA was extracted using PicoPure RNA isolation Kit (Life Technologies, CA) or AllPrep DNA/RNA Micro Kit (Qiagen, Germany) with an on-column DNaseI digestion (Qiagen, Germany). cDNA was prepared using SuperScript III (Life Technologies, CA) and random hexamer primers (Life Technologies, CA).

    Article Title: Proteomic Detection of Non-Annotated Protein-Coding Genes in Pseudomonas fluorescens Pf0-1
    Article Snippet: RNA was extracted from P. fluorescens Pf0-1 using an RNeasy Mini Kit, and on-column DNaseI digestion (Qiagen). .. RNA so recovered was treated with RQ1 DNase (37°C, 1 h) (Promega), and subsequently purified using an RNeasy Mini Kit column.

    Synthesized:

    Article Title: Protein Phosphatase 2A Holoenzyme Is Targeted to Peroxisomes by Piggybacking and Positively Affects Peroxisomal β-Oxidation
    Article Snippet: Total RNA was isolated using the RNeasy Plant Mini Kit and treated with on-column DNaseI digestion (Qiagen). .. Total RNA was isolated using the RNeasy Plant Mini Kit and treated with on-column DNaseI digestion (Qiagen).

    Article Title: The Pancreas Is Altered by In Utero Androgen Exposure: Implications for Clinical Conditions Such as Polycystic Ovary Syndrome (PCOS)
    Article Snippet: Frozen fetal pancreas was homogenised in RLT buffer (including 2-mercaptoethanol, 1% v/v) (Qiagen, Crawley, UK) and RNA was extracted using RNeasy minispin columns following the manufacturer’s protocols, including on-column DNaseI digestion (Qiagen, Crawley, UK). .. RNA concentrations were determined using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Loughborough, UK), with gel electrophoresis used to monitor quality.

    Article Title: Fine Mapping of an Epilepsy Modifier Gene on Mouse Chromosome 19
    Article Snippet: Whole brain RNA was isolated from 6 week old male C57BL/6J and SJL/J mice (n=5 per strain) using the RNeasy Kit with on-column DNaseI digestion according to the manufacturer’s instructions (Qiagen). .. Following total RNA isolation a second DNase treatment was performed with Turbo DNase according to the manufacturer’s instructions (Ambion).

    Quantitative RT-PCR:

    Article Title: Functional and molecular characterisation of EO771.LMB tumours, a new C57BL/6-mouse-derived model of spontaneously metastatic mammary cancer
    Article Snippet: Paragraph title: Quantitative real-time RT-PCR ... Total RNA was subsequently re-purified using RNeasy mini-columns with on-column DNaseI digestion (Qiagen, Doncaster, Victoria, Australia).

    Article Title: PLATINUM SENSITIVE 2 LIKE impacts growth, root morphology, seed set, and stress responses
    Article Snippet: Root measurements were accomplished using Image J ( https://imagej.nih.gov/ij/index.html ). .. For qRT-PCR, total RNA was extracted using RNAeasy Plant Mini Kit and treated with on-column DNaseI digestion (Qiagen, Hilden, Germany). .. One μg RNA was reverse-transcribed using the High Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA, USA) to generate first-strand cDNA in a 20 μL reaction volume.

    Article Title: Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development
    Article Snippet: Total RNA was extracted using PicoPure RNA isolation Kit (Life Technologies, CA) or AllPrep DNA/RNA Micro Kit (Qiagen, Germany) with an on-column DNaseI digestion (Qiagen, Germany). cDNA was prepared using SuperScript III (Life Technologies, CA) and random hexamer primers (Life Technologies, CA). .. RT-q PCR reactions were performed using Kapa Sybr Fast qPCR kit (Kapa Biosystems, MA).

    Article Title: Fine Mapping of an Epilepsy Modifier Gene on Mouse Chromosome 19
    Article Snippet: Paragraph title: Real-Time RT-PCR ... Whole brain RNA was isolated from 6 week old male C57BL/6J and SJL/J mice (n=5 per strain) using the RNeasy Kit with on-column DNaseI digestion according to the manufacturer’s instructions (Qiagen).

    SYBR Green Assay:

    Article Title: Functional and molecular characterisation of EO771.LMB tumours, a new C57BL/6-mouse-derived model of spontaneously metastatic mammary cancer
    Article Snippet: Total RNA was subsequently re-purified using RNeasy mini-columns with on-column DNaseI digestion (Qiagen, Doncaster, Victoria, Australia). .. For cell lines, total RNA was isolated using RNeasy mini-kits with on-column DNaseI digestion (Qiagen).

    Article Title: Peritoneal VEGF-A expression is regulated by TGF-β1 through an ID1 pathway in women with endometriosis
    Article Snippet: RNA was extracted using the RNeasy Mini kit with on-column DNaseI digestion according to the manufacturer’s instructions (Qiagen, West Sussex, UK). .. RNA was extracted using the RNeasy Mini kit with on-column DNaseI digestion according to the manufacturer’s instructions (Qiagen, West Sussex, UK).

    Article Title: Fine Mapping of an Epilepsy Modifier Gene on Mouse Chromosome 19
    Article Snippet: Whole brain RNA was isolated from 6 week old male C57BL/6J and SJL/J mice (n=5 per strain) using the RNeasy Kit with on-column DNaseI digestion according to the manufacturer’s instructions (Qiagen). .. First strand cDNA was synthesized from 1–2 micrograms of template RNA using oligo(dT)20 primers with the Superscript III first strand synthesis system (Invitrogen).

    Random Hexamer Labeling:

    Article Title: Expression of Innate Immunity Genes and Damage of Primary Human Pancreatic Islets by Epidemic Strains of Echovirus: Implication for Post-Virus Islet Autoimmunity
    Article Snippet: Total RNA from islets was extracted with RNAeasy Mini kit with on-column DNaseI digestion (Qiagen) with G DNA eliminator column to remove the genomic DNA. .. Total RNA from islets was extracted with RNAeasy Mini kit with on-column DNaseI digestion (Qiagen) with G DNA eliminator column to remove the genomic DNA.

    Article Title: Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development
    Article Snippet: For single epiblast experiments, embryos were dissected as previously described ( ). .. Total RNA was extracted using PicoPure RNA isolation Kit (Life Technologies, CA) or AllPrep DNA/RNA Micro Kit (Qiagen, Germany) with an on-column DNaseI digestion (Qiagen, Germany). cDNA was prepared using SuperScript III (Life Technologies, CA) and random hexamer primers (Life Technologies, CA). .. RT-q PCR reactions were performed using Kapa Sybr Fast qPCR kit (Kapa Biosystems, MA).

    Cell Culture:

    Article Title: A common variant in the 3?UTR of the GRIK4 glutamate receptor gene affects transcript abundance and protects against bipolar disorder
    Article Snippet: After 24 h of transfection in serum-free conditions, the growth medium was replaced by 10% fetal bovine serum-supplemented Dulbecco's modified Eagle medium. .. Cells were cultured for another 24 h and then harvested using trypsin/versene in preparation for total RNA extraction using a RNeasy Mini Kit and on-column DNaseI digestion (Qiagen). .. Frozen samples from histologically characterized normal brain tissues that were authorized for ethically approved scientific investigation were obtained from the Medical Research Council Sudden Death Brain & Tissue Bank, Edinburgh ( ) and genotyped for indel status.

    Expressing:

    Article Title: Acute knockdown of the insulin receptor or its substrates Irs1 and 2 in 3T3-L1 adipocytes suppresses adiponectin production
    Article Snippet: Total RNA was extracted using an RNeasy mini kit including on-column DNAseI digestion (Qiagen). .. Fluorescence was monitored and analysed in an ABI Prism 7900 HT sequence detection system (Applied Biosystems).

    Article Title: Functional and molecular characterisation of EO771.LMB tumours, a new C57BL/6-mouse-derived model of spontaneously metastatic mammary cancer
    Article Snippet: Total RNA was subsequently re-purified using RNeasy mini-columns with on-column DNaseI digestion (Qiagen, Doncaster, Victoria, Australia). .. For cell lines, total RNA was isolated using RNeasy mini-kits with on-column DNaseI digestion (Qiagen).

    Article Title: PLATINUM SENSITIVE 2 LIKE impacts growth, root morphology, seed set, and stress responses
    Article Snippet: For qRT-PCR, total RNA was extracted using RNAeasy Plant Mini Kit and treated with on-column DNaseI digestion (Qiagen, Hilden, Germany). .. For qRT-PCR, total RNA was extracted using RNAeasy Plant Mini Kit and treated with on-column DNaseI digestion (Qiagen, Hilden, Germany).

    Article Title: Expression of Innate Immunity Genes and Damage of Primary Human Pancreatic Islets by Epidemic Strains of Echovirus: Implication for Post-Virus Islet Autoimmunity
    Article Snippet: Paragraph title: Innate Immunity Gene Expression in Echovirus-infected Islets ... Total RNA from islets was extracted with RNAeasy Mini kit with on-column DNaseI digestion (Qiagen) with G DNA eliminator column to remove the genomic DNA.

    Article Title: Developmental programming of polycystic ovary syndrome (PCOS): prenatal androgens establish pancreatic islet α/β cell ratio and subsequent insulin secretion
    Article Snippet: Paragraph title: Examination of Gene Expression by Quantitative (Q) RT-PCR ... Frozen fetal pancreas and offspring liver and striated muscle was homogenised in RLT buffer (including 2-mercaptoethanol, 1% v/v) (Qiagen, Crawley, UK) and RNA was extracted using RNeasy minispin columns following the manufacturer’s protocols, including on-column DNaseI digestion (Qiagen, Crawley, UK).

    Article Title: The Pancreas Is Altered by In Utero Androgen Exposure: Implications for Clinical Conditions Such as Polycystic Ovary Syndrome (PCOS)
    Article Snippet: Paragraph title: Examination of Gene Expression by Quantitative (Q) RT-PCR ... Frozen fetal pancreas was homogenised in RLT buffer (including 2-mercaptoethanol, 1% v/v) (Qiagen, Crawley, UK) and RNA was extracted using RNeasy minispin columns following the manufacturer’s protocols, including on-column DNaseI digestion (Qiagen, Crawley, UK).

    Article Title: A common variant in the 3?UTR of the GRIK4 glutamate receptor gene affects transcript abundance and protects against bipolar disorder
    Article Snippet: Equal quantities (as determined by spectroscopic DNA quantification) of the two expression constructs were combined and transfected into HEK293 cells using Lipofectamine 2000 (Invitrogen) according to manufacturer's directions. .. Cells were cultured for another 24 h and then harvested using trypsin/versene in preparation for total RNA extraction using a RNeasy Mini Kit and on-column DNaseI digestion (Qiagen).

    Article Title: Circadian and light-driven regulation of rod dark adaptation
    Article Snippet: Total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen) with on-column DNaseI digestion (Qiagen). .. Total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen) with on-column DNaseI digestion (Qiagen).

    Modification:

    Article Title: A common variant in the 3?UTR of the GRIK4 glutamate receptor gene affects transcript abundance and protects against bipolar disorder
    Article Snippet: After 24 h of transfection in serum-free conditions, the growth medium was replaced by 10% fetal bovine serum-supplemented Dulbecco's modified Eagle medium. .. Cells were cultured for another 24 h and then harvested using trypsin/versene in preparation for total RNA extraction using a RNeasy Mini Kit and on-column DNaseI digestion (Qiagen).

    Transfection:

    Article Title: A common variant in the 3?UTR of the GRIK4 glutamate receptor gene affects transcript abundance and protects against bipolar disorder
    Article Snippet: Paragraph title: Transient Transfection Experiments. ... Cells were cultured for another 24 h and then harvested using trypsin/versene in preparation for total RNA extraction using a RNeasy Mini Kit and on-column DNaseI digestion (Qiagen).

    Ligation:

    Article Title: Hybrid Mice Reveal Parent-of-Origin and Cis- and Trans-Regulatory Effects in the Retina
    Article Snippet: Total RNA was extracted using TRIzol (Invitrogen) and purified using the RNeasy Mini Kit (Qiagen) with on-column DNaseI digestion (Qiagen). .. Total RNA was extracted using TRIzol (Invitrogen) and purified using the RNeasy Mini Kit (Qiagen) with on-column DNaseI digestion (Qiagen).

    Infection:

    Article Title: Expression of Innate Immunity Genes and Damage of Primary Human Pancreatic Islets by Epidemic Strains of Echovirus: Implication for Post-Virus Islet Autoimmunity
    Article Snippet: Three days post infection isolated human pancreatic islets were washed in PBS and lysed by using RLT buffer (Qiagen, Sollentuna, Sweden) on QIAshredder spin columns (Qiagen, Sollentuna, Sweden). .. Total RNA from islets was extracted with RNAeasy Mini kit with on-column DNaseI digestion (Qiagen) with G DNA eliminator column to remove the genomic DNA.

    other:

    Article Title: Transforming Growth Factor-β Induced Warburg-Like Metabolic Reprogramming May Underpin the Development of Peritoneal Endometriosis
    Article Snippet: RNA was extracted using the RNeasy Mini kit with on-column DNaseI digestion (QIAGEN) according to the manufacturer's instructions.

    Article Title: PLATINUM SENSITIVE 2 LIKE impacts growth, root morphology, seed set, and stress responses
    Article Snippet: Total RNA was extracted using RNAeasy Plant Mini Kit and treated with on-column DNaseI digestion.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Developmental programming of polycystic ovary syndrome (PCOS): prenatal androgens establish pancreatic islet α/β cell ratio and subsequent insulin secretion
    Article Snippet: Paragraph title: Examination of Gene Expression by Quantitative (Q) RT-PCR ... Frozen fetal pancreas and offspring liver and striated muscle was homogenised in RLT buffer (including 2-mercaptoethanol, 1% v/v) (Qiagen, Crawley, UK) and RNA was extracted using RNeasy minispin columns following the manufacturer’s protocols, including on-column DNaseI digestion (Qiagen, Crawley, UK).

    Article Title: Proteomic Detection of Non-Annotated Protein-Coding Genes in Pseudomonas fluorescens Pf0-1
    Article Snippet: Paragraph title: RNA Isolation and RT-PCR ... RNA was extracted from P. fluorescens Pf0-1 using an RNeasy Mini Kit, and on-column DNaseI digestion (Qiagen).

    Article Title: The Pancreas Is Altered by In Utero Androgen Exposure: Implications for Clinical Conditions Such as Polycystic Ovary Syndrome (PCOS)
    Article Snippet: Paragraph title: Examination of Gene Expression by Quantitative (Q) RT-PCR ... Frozen fetal pancreas was homogenised in RLT buffer (including 2-mercaptoethanol, 1% v/v) (Qiagen, Crawley, UK) and RNA was extracted using RNeasy minispin columns following the manufacturer’s protocols, including on-column DNaseI digestion (Qiagen, Crawley, UK).

    Article Title: Mutation of the cytosolic ribosomal protein-encoding RPS10B gene affects shoot meristematic function in Arabidopsis
    Article Snippet: Paragraph title: RNA isolation, RT-PCR, cloning ... Total RNA was extracted using the RNeasy plant miniprep kit with on-column DNaseI digestion (Qiagen, Hilden, Germany) from about 100 mg tissue powder, obtained from 10 pooled 1-cm primary inflorescence stem base segments per genotype, from bolting plants of about 25 cm height. cDNA synthesis was performed from 1 μg total RNA in a total volume of 10 μl with SuperscriptII (Invitrogen, Life Technologies, Carlsbad, CA) and oligo-dT primer.

    RNA Sequencing Assay:

    Article Title: Circadian and light-driven regulation of rod dark adaptation
    Article Snippet: Paragraph title: RNA-seq ... Total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen) with on-column DNaseI digestion (Qiagen).

    Fluorescence:

    Article Title: Acute knockdown of the insulin receptor or its substrates Irs1 and 2 in 3T3-L1 adipocytes suppresses adiponectin production
    Article Snippet: Total RNA was extracted using an RNeasy mini kit including on-column DNAseI digestion (Qiagen). .. Taqman real time PCR was performed starting with 10 ng of cDNA, sense and antisense oligonucleotides (333 nM) and a 5′-[6FAM], 3′-[TAMRA]- labeled fluorogenic probe (167 nM) (Sigma-Aldrich) using PCR Taqman Mastermix (Applied Biosystems) in a final volume of 12 μl.

    Magnetic Beads:

    Article Title: Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix
    Article Snippet: Cells were incubated overnight, medium changed after 24 hrs and incubated for another 1 or 2 days until confluence, when RNA was extracted. .. RNA was extracted using RNeasy mini kit (Qiagen) following manufacturer instructions, DNA was removed with on-column DNaseI digestion (RNase-free DNaseI, Qiagen) and total RNA was analyzed for quality and quantity using an Agilent Bioanalyzer (Agilent RNA Nano chips, Agilent). polyA mRNA was purified and fragmented using poly-T oligo attached magnetic beads in accordance to manufacturer instructions, (Illumina). .. The cleaved RNA fragments were primed with random hexamers into first strand cDNA followed by reverse transcription using reverse transcriptase (SuperScriptII reverse transcriptase, Invitrogen) and random primers.

    Isolation:

    Article Title: Acute knockdown of the insulin receptor or its substrates Irs1 and 2 in 3T3-L1 adipocytes suppresses adiponectin production
    Article Snippet: Paragraph title: RNA isolation and Real-time PCR analysis ... Total RNA was extracted using an RNeasy mini kit including on-column DNAseI digestion (Qiagen).

    Article Title: The Formation and Sequestration of Nonendogenous Ketocarotenoids in Transgenic Nicotiana glauca
    Article Snippet: Paragraph title: DNA and RNA Isolation and Quantitative Analysis ... RNA was extracted using the RNeasy reagents and protocol, including on-column DNaseI digestion (Qiagen).

    Article Title: Functional and molecular characterisation of EO771.LMB tumours, a new C57BL/6-mouse-derived model of spontaneously metastatic mammary cancer
    Article Snippet: A representative sample of primary tumour was lysed in Trizol reagent using a FastPrep automated bench-top homogeniser (MP Biomedical, Seven Hills, NSW, Australia), and total RNA isolated in accordance with the manufacturer’s instructions (Life Technologies). .. Total RNA was subsequently re-purified using RNeasy mini-columns with on-column DNaseI digestion (Qiagen, Doncaster, Victoria, Australia).

    Article Title: Functional and molecular characterisation of EO771.LMB tumours, a new C57BL/6-mouse-derived model of spontaneously metastatic mammary cancer
    Article Snippet: Total RNA was subsequently re-purified using RNeasy mini-columns with on-column DNaseI digestion (Qiagen, Doncaster, Victoria, Australia). .. For cell lines, total RNA was isolated using RNeasy mini-kits with on-column DNaseI digestion (Qiagen). .. RNA quality was determined using an RNA6000 Nano chip and 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). cDNA was synthesised using SuperscriptIII reverse transcriptase (Life Technologies). qPCR was completed using either inventoried TaqMan gene expression assays (ERα, ERβ, PR, Erb-b2, Cd36, MMP-3, Glycam1) in a 15 μl reaction volume (Life Technologies) or using SYBR-green reagent (Life Technologies) in a 15 μl reaction volume in conjunction with the primers listed in supplementary material Table S4 , as previously described ( ).

    Article Title: Expression of Innate Immunity Genes and Damage of Primary Human Pancreatic Islets by Epidemic Strains of Echovirus: Implication for Post-Virus Islet Autoimmunity
    Article Snippet: Three days post infection isolated human pancreatic islets were washed in PBS and lysed by using RLT buffer (Qiagen, Sollentuna, Sweden) on QIAshredder spin columns (Qiagen, Sollentuna, Sweden). .. Total RNA from islets was extracted with RNAeasy Mini kit with on-column DNaseI digestion (Qiagen) with G DNA eliminator column to remove the genomic DNA.

    Article Title: Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development
    Article Snippet: For single epiblast experiments, embryos were dissected as previously described ( ). .. Total RNA was extracted using PicoPure RNA isolation Kit (Life Technologies, CA) or AllPrep DNA/RNA Micro Kit (Qiagen, Germany) with an on-column DNaseI digestion (Qiagen, Germany). cDNA was prepared using SuperScript III (Life Technologies, CA) and random hexamer primers (Life Technologies, CA). .. RT-q PCR reactions were performed using Kapa Sybr Fast qPCR kit (Kapa Biosystems, MA).

    Article Title: Proteomic Detection of Non-Annotated Protein-Coding Genes in Pseudomonas fluorescens Pf0-1
    Article Snippet: Paragraph title: RNA Isolation and RT-PCR ... RNA was extracted from P. fluorescens Pf0-1 using an RNeasy Mini Kit, and on-column DNaseI digestion (Qiagen).

    Article Title: Protein Phosphatase 2A Holoenzyme Is Targeted to Peroxisomes by Piggybacking and Positively Affects Peroxisomal β-Oxidation
    Article Snippet: Seeds were sown and stratified for 2 d, then kept in continuous light for 7 d. Length of the primary root was measured using ImageJ ( ; ). .. Total RNA was isolated using the RNeasy Plant Mini Kit and treated with on-column DNaseI digestion (Qiagen). .. Afterward, RNA was treated in solution with DNaseI (Life Technologies) and precipitated by ammonium acetate (7.5 m ) and ethanol.

    Article Title: Mutation of the cytosolic ribosomal protein-encoding RPS10B gene affects shoot meristematic function in Arabidopsis
    Article Snippet: Paragraph title: RNA isolation, RT-PCR, cloning ... Total RNA was extracted using the RNeasy plant miniprep kit with on-column DNaseI digestion (Qiagen, Hilden, Germany) from about 100 mg tissue powder, obtained from 10 pooled 1-cm primary inflorescence stem base segments per genotype, from bolting plants of about 25 cm height. cDNA synthesis was performed from 1 μg total RNA in a total volume of 10 μl with SuperscriptII (Invitrogen, Life Technologies, Carlsbad, CA) and oligo-dT primer.

    Article Title: Fine Mapping of an Epilepsy Modifier Gene on Mouse Chromosome 19
    Article Snippet: Sequences were compared using Sequencher software (GeneCodes, Ann Arbor, MI). .. Whole brain RNA was isolated from 6 week old male C57BL/6J and SJL/J mice (n=5 per strain) using the RNeasy Kit with on-column DNaseI digestion according to the manufacturer’s instructions (Qiagen). .. Following total RNA isolation a second DNase treatment was performed with Turbo DNase according to the manufacturer’s instructions (Ambion).

    Polymerase Chain Reaction:

    Article Title: Acute knockdown of the insulin receptor or its substrates Irs1 and 2 in 3T3-L1 adipocytes suppresses adiponectin production
    Article Snippet: Total RNA was extracted using an RNeasy mini kit including on-column DNAseI digestion (Qiagen). .. Total RNA was extracted using an RNeasy mini kit including on-column DNAseI digestion (Qiagen).

    Article Title: Functional and molecular characterisation of EO771.LMB tumours, a new C57BL/6-mouse-derived model of spontaneously metastatic mammary cancer
    Article Snippet: Total RNA was subsequently re-purified using RNeasy mini-columns with on-column DNaseI digestion (Qiagen, Doncaster, Victoria, Australia). .. Total RNA was subsequently re-purified using RNeasy mini-columns with on-column DNaseI digestion (Qiagen, Doncaster, Victoria, Australia).

    Article Title: Proteomic Detection of Non-Annotated Protein-Coding Genes in Pseudomonas fluorescens Pf0-1
    Article Snippet: RNA was extracted from P. fluorescens Pf0-1 using an RNeasy Mini Kit, and on-column DNaseI digestion (Qiagen). .. RNA so recovered was treated with RQ1 DNase (37°C, 1 h) (Promega), and subsequently purified using an RNeasy Mini Kit column.

    Article Title: Protein Phosphatase 2A Holoenzyme Is Targeted to Peroxisomes by Piggybacking and Positively Affects Peroxisomal β-Oxidation
    Article Snippet: Paragraph title: -PCR ... Total RNA was isolated using the RNeasy Plant Mini Kit and treated with on-column DNaseI digestion (Qiagen).

    Article Title: Fine Mapping of an Epilepsy Modifier Gene on Mouse Chromosome 19
    Article Snippet: Whole brain RNA was isolated from 6 week old male C57BL/6J and SJL/J mice (n=5 per strain) using the RNeasy Kit with on-column DNaseI digestion according to the manufacturer’s instructions (Qiagen). .. First strand cDNA was synthesized from 1–2 micrograms of template RNA using oligo(dT)20 primers with the Superscript III first strand synthesis system (Invitrogen).

    RNA Extraction:

    Article Title: Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix
    Article Snippet: Paragraph title: RNA extraction and processing ... RNA was extracted using RNeasy mini kit (Qiagen) following manufacturer instructions, DNA was removed with on-column DNaseI digestion (RNase-free DNaseI, Qiagen) and total RNA was analyzed for quality and quantity using an Agilent Bioanalyzer (Agilent RNA Nano chips, Agilent). polyA mRNA was purified and fragmented using poly-T oligo attached magnetic beads in accordance to manufacturer instructions, (Illumina).

    Article Title: The Pancreas Is Altered by In Utero Androgen Exposure: Implications for Clinical Conditions Such as Polycystic Ovary Syndrome (PCOS)
    Article Snippet: For RNA extraction, frozen adult tissue samples were homogenized with 1 ml TRI-reagent (Sigma Aldrich, UK) following the manufacturer’s instructions and the RNA was dissolved in 20 µl RNase-free water. .. Frozen fetal pancreas was homogenised in RLT buffer (including 2-mercaptoethanol, 1% v/v) (Qiagen, Crawley, UK) and RNA was extracted using RNeasy minispin columns following the manufacturer’s protocols, including on-column DNaseI digestion (Qiagen, Crawley, UK).

    Article Title: A common variant in the 3?UTR of the GRIK4 glutamate receptor gene affects transcript abundance and protects against bipolar disorder
    Article Snippet: After 24 h of transfection in serum-free conditions, the growth medium was replaced by 10% fetal bovine serum-supplemented Dulbecco's modified Eagle medium. .. Cells were cultured for another 24 h and then harvested using trypsin/versene in preparation for total RNA extraction using a RNeasy Mini Kit and on-column DNaseI digestion (Qiagen). .. Frozen samples from histologically characterized normal brain tissues that were authorized for ethically approved scientific investigation were obtained from the Medical Research Council Sudden Death Brain & Tissue Bank, Edinburgh ( ) and genotyped for indel status.

    Labeling:

    Article Title: Acute knockdown of the insulin receptor or its substrates Irs1 and 2 in 3T3-L1 adipocytes suppresses adiponectin production
    Article Snippet: Total RNA was extracted using an RNeasy mini kit including on-column DNAseI digestion (Qiagen). .. Total RNA was extracted using an RNeasy mini kit including on-column DNAseI digestion (Qiagen).

    Purification:

    Article Title: Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix
    Article Snippet: Cells were incubated overnight, medium changed after 24 hrs and incubated for another 1 or 2 days until confluence, when RNA was extracted. .. RNA was extracted using RNeasy mini kit (Qiagen) following manufacturer instructions, DNA was removed with on-column DNaseI digestion (RNase-free DNaseI, Qiagen) and total RNA was analyzed for quality and quantity using an Agilent Bioanalyzer (Agilent RNA Nano chips, Agilent). polyA mRNA was purified and fragmented using poly-T oligo attached magnetic beads in accordance to manufacturer instructions, (Illumina). .. The cleaved RNA fragments were primed with random hexamers into first strand cDNA followed by reverse transcription using reverse transcriptase (SuperScriptII reverse transcriptase, Invitrogen) and random primers.

    Article Title: Circadian and light-driven regulation of rod dark adaptation
    Article Snippet: The anterior portion of the eye including the lens was removed, and the remaining tissue (posterior sclera, choroid, RPE, and retina) was rinsed in cold sterile HBSS with calcium and magnesium (Gibco) and stored in TRIzol (Invitrogen) at −80 °C. .. Total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen) with on-column DNaseI digestion (Qiagen). .. Integrity of total RNA was verified on a Agilent 2100 Bioanalyzer.

    Article Title: Hybrid Mice Reveal Parent-of-Origin and Cis- and Trans-Regulatory Effects in the Retina
    Article Snippet: Retinas were dissected in cold sterile HBSS with calcium and magnesium (Gibco) and stored at −80°C until use. .. Total RNA was extracted using TRIzol (Invitrogen) and purified using the RNeasy Mini Kit (Qiagen) with on-column DNaseI digestion (Qiagen). .. Integrity of total RNA was verified on the Agilent 2100 Bioanalyzer.

    Sequencing:

    Article Title: Acute knockdown of the insulin receptor or its substrates Irs1 and 2 in 3T3-L1 adipocytes suppresses adiponectin production
    Article Snippet: Total RNA was extracted using an RNeasy mini kit including on-column DNAseI digestion (Qiagen). .. Taqman real time PCR was performed starting with 10 ng of cDNA, sense and antisense oligonucleotides (333 nM) and a 5′-[6FAM], 3′-[TAMRA]- labeled fluorogenic probe (167 nM) (Sigma-Aldrich) using PCR Taqman Mastermix (Applied Biosystems) in a final volume of 12 μl.

    Article Title: PLATINUM SENSITIVE 2 LIKE impacts growth, root morphology, seed set, and stress responses
    Article Snippet: For qRT-PCR, total RNA was extracted using RNAeasy Plant Mini Kit and treated with on-column DNaseI digestion (Qiagen, Hilden, Germany). .. For qRT-PCR, total RNA was extracted using RNAeasy Plant Mini Kit and treated with on-column DNaseI digestion (Qiagen, Hilden, Germany).

    Article Title: A common variant in the 3?UTR of the GRIK4 glutamate receptor gene affects transcript abundance and protects against bipolar disorder
    Article Snippet: The cloned GRIK4 sequences compose the majority of the final coding exon sequence and ≈1 kb of 3′UTR. .. Cells were cultured for another 24 h and then harvested using trypsin/versene in preparation for total RNA extraction using a RNeasy Mini Kit and on-column DNaseI digestion (Qiagen).

    Article Title: Circadian and light-driven regulation of rod dark adaptation
    Article Snippet: Total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen) with on-column DNaseI digestion (Qiagen). .. Total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen) with on-column DNaseI digestion (Qiagen).

    Article Title: Hybrid Mice Reveal Parent-of-Origin and Cis- and Trans-Regulatory Effects in the Retina
    Article Snippet: Paragraph title: Sample collection and sequencing ... Total RNA was extracted using TRIzol (Invitrogen) and purified using the RNeasy Mini Kit (Qiagen) with on-column DNaseI digestion (Qiagen).

    Construct:

    Article Title: A common variant in the 3?UTR of the GRIK4 glutamate receptor gene affects transcript abundance and protects against bipolar disorder
    Article Snippet: Equal quantities (as determined by spectroscopic DNA quantification) of the two expression constructs were combined and transfected into HEK293 cells using Lipofectamine 2000 (Invitrogen) according to manufacturer's directions. .. Cells were cultured for another 24 h and then harvested using trypsin/versene in preparation for total RNA extraction using a RNeasy Mini Kit and on-column DNaseI digestion (Qiagen).

    cDNA Library Assay:

    Article Title: Circadian and light-driven regulation of rod dark adaptation
    Article Snippet: Total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen) with on-column DNaseI digestion (Qiagen). .. Total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen) with on-column DNaseI digestion (Qiagen).

    Mouse Assay:

    Article Title: Hybrid Mice Reveal Parent-of-Origin and Cis- and Trans-Regulatory Effects in the Retina
    Article Snippet: Each biological replicate consisted of a pool of 6–8 retinas from 8 week old male mice. .. Total RNA was extracted using TRIzol (Invitrogen) and purified using the RNeasy Mini Kit (Qiagen) with on-column DNaseI digestion (Qiagen).

    Article Title: Fine Mapping of an Epilepsy Modifier Gene on Mouse Chromosome 19
    Article Snippet: Sequences were compared using Sequencher software (GeneCodes, Ann Arbor, MI). .. Whole brain RNA was isolated from 6 week old male C57BL/6J and SJL/J mice (n=5 per strain) using the RNeasy Kit with on-column DNaseI digestion according to the manufacturer’s instructions (Qiagen). .. Following total RNA isolation a second DNase treatment was performed with Turbo DNase according to the manufacturer’s instructions (Ambion).

    Chromatin Immunoprecipitation:

    Article Title: Functional and molecular characterisation of EO771.LMB tumours, a new C57BL/6-mouse-derived model of spontaneously metastatic mammary cancer
    Article Snippet: Total RNA was subsequently re-purified using RNeasy mini-columns with on-column DNaseI digestion (Qiagen, Doncaster, Victoria, Australia). .. For cell lines, total RNA was isolated using RNeasy mini-kits with on-column DNaseI digestion (Qiagen).

    Plasmid Preparation:

    Article Title: A common variant in the 3?UTR of the GRIK4 glutamate receptor gene affects transcript abundance and protects against bipolar disorder
    Article Snippet: Plasmid inserts were fully sequenced to ensure correct incorporation of GRIK4 insertion or deletion sequences. .. Cells were cultured for another 24 h and then harvested using trypsin/versene in preparation for total RNA extraction using a RNeasy Mini Kit and on-column DNaseI digestion (Qiagen).

    Software:

    Article Title: PLATINUM SENSITIVE 2 LIKE impacts growth, root morphology, seed set, and stress responses
    Article Snippet: For qRT-PCR, total RNA was extracted using RNAeasy Plant Mini Kit and treated with on-column DNaseI digestion (Qiagen, Hilden, Germany). .. Primers were predesigned TaqMan Gene expression assays ( ).

    Real-time Polymerase Chain Reaction:

    Article Title: Acute knockdown of the insulin receptor or its substrates Irs1 and 2 in 3T3-L1 adipocytes suppresses adiponectin production
    Article Snippet: Paragraph title: RNA isolation and Real-time PCR analysis ... Total RNA was extracted using an RNeasy mini kit including on-column DNAseI digestion (Qiagen).

    Article Title: Functional and molecular characterisation of EO771.LMB tumours, a new C57BL/6-mouse-derived model of spontaneously metastatic mammary cancer
    Article Snippet: Total RNA was subsequently re-purified using RNeasy mini-columns with on-column DNaseI digestion (Qiagen, Doncaster, Victoria, Australia). .. Total RNA was subsequently re-purified using RNeasy mini-columns with on-column DNaseI digestion (Qiagen, Doncaster, Victoria, Australia).

    Article Title: Peritoneal VEGF-A expression is regulated by TGF-β1 through an ID1 pathway in women with endometriosis
    Article Snippet: RNA was extracted using the RNeasy Mini kit with on-column DNaseI digestion according to the manufacturer’s instructions (Qiagen, West Sussex, UK). .. RNA was extracted using the RNeasy Mini kit with on-column DNaseI digestion according to the manufacturer’s instructions (Qiagen, West Sussex, UK).

    Article Title: PLATINUM SENSITIVE 2 LIKE impacts growth, root morphology, seed set, and stress responses
    Article Snippet: For qRT-PCR, total RNA was extracted using RNAeasy Plant Mini Kit and treated with on-column DNaseI digestion (Qiagen, Hilden, Germany). .. For qRT-PCR, total RNA was extracted using RNAeasy Plant Mini Kit and treated with on-column DNaseI digestion (Qiagen, Hilden, Germany).

    Article Title: Functional and molecular characterisation of EO771.LMB tumours, a new C57BL/6-mouse-derived model of spontaneously metastatic mammary cancer
    Article Snippet: For cell lines, total RNA was isolated using RNeasy mini-kits with on-column DNaseI digestion (Qiagen). .. For cell lines, total RNA was isolated using RNeasy mini-kits with on-column DNaseI digestion (Qiagen).

    Article Title: Developmental programming of polycystic ovary syndrome (PCOS): prenatal androgens establish pancreatic islet α/β cell ratio and subsequent insulin secretion
    Article Snippet: Frozen fetal pancreas and offspring liver and striated muscle was homogenised in RLT buffer (including 2-mercaptoethanol, 1% v/v) (Qiagen, Crawley, UK) and RNA was extracted using RNeasy minispin columns following the manufacturer’s protocols, including on-column DNaseI digestion (Qiagen, Crawley, UK). .. Frozen fetal pancreas and offspring liver and striated muscle was homogenised in RLT buffer (including 2-mercaptoethanol, 1% v/v) (Qiagen, Crawley, UK) and RNA was extracted using RNeasy minispin columns following the manufacturer’s protocols, including on-column DNaseI digestion (Qiagen, Crawley, UK).

    Article Title: Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development
    Article Snippet: Paragraph title: RNA isolation, reverse transcription and qPCR ... Total RNA was extracted using PicoPure RNA isolation Kit (Life Technologies, CA) or AllPrep DNA/RNA Micro Kit (Qiagen, Germany) with an on-column DNaseI digestion (Qiagen, Germany). cDNA was prepared using SuperScript III (Life Technologies, CA) and random hexamer primers (Life Technologies, CA).

    Article Title: Protein Phosphatase 2A Holoenzyme Is Targeted to Peroxisomes by Piggybacking and Positively Affects Peroxisomal β-Oxidation
    Article Snippet: Total RNA was isolated using the RNeasy Plant Mini Kit and treated with on-column DNaseI digestion (Qiagen). .. The was synthesized using the High Capacity cDNA Archive Kit (Applied Biosystems).

    Article Title: Fine Mapping of an Epilepsy Modifier Gene on Mouse Chromosome 19
    Article Snippet: Whole brain RNA was isolated from 6 week old male C57BL/6J and SJL/J mice (n=5 per strain) using the RNeasy Kit with on-column DNaseI digestion according to the manufacturer’s instructions (Qiagen). .. First strand cDNA was synthesized from 1–2 micrograms of template RNA using oligo(dT)20 primers with the Superscript III first strand synthesis system (Invitrogen).

    Negative Control:

    Article Title: Proteomic Detection of Non-Annotated Protein-Coding Genes in Pseudomonas fluorescens Pf0-1
    Article Snippet: RNA was extracted from P. fluorescens Pf0-1 using an RNeasy Mini Kit, and on-column DNaseI digestion (Qiagen). .. RNA was extracted from P. fluorescens Pf0-1 using an RNeasy Mini Kit, and on-column DNaseI digestion (Qiagen).

    Selection:

    Article Title: Circadian and light-driven regulation of rod dark adaptation
    Article Snippet: Total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen) with on-column DNaseI digestion (Qiagen). .. Total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen) with on-column DNaseI digestion (Qiagen).

    Concentration Assay:

    Article Title: The Formation and Sequestration of Nonendogenous Ketocarotenoids in Transgenic Nicotiana glauca
    Article Snippet: RNA was extracted using the RNeasy reagents and protocol, including on-column DNaseI digestion (Qiagen). .. The analysis of copy number relied upon the relative amplification of the transgenes and an endogenous single-copy gene, Pds .

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  • 99
    Qiagen on column dnase i digestion
    Summary of  in vitro  primer extension and DNase I and KMnO 4  footprinting. ( A ) Sequence of P vpsL  from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4  footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4  cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.
    On Column Dnase I Digestion, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/on column dnase i digestion/product/Qiagen
    Average 99 stars, based on 235 article reviews
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    99
    Qiagen column dnase digestion
    Summary of  in vitro  primer extension and DNase I and KMnO 4  footprinting. ( A ) Sequence of P vpsL  from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4  footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4  cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.
    Column Dnase Digestion, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/column dnase digestion/product/Qiagen
    Average 99 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    column dnase digestion - by Bioz Stars, 2019-12
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    93
    Qiagen dnase on column digestion
    Summary of  in vitro  primer extension and DNase I and KMnO 4  footprinting. ( A ) Sequence of P vpsL  from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4  footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4  cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.
    Dnase On Column Digestion, supplied by Qiagen, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase on column digestion/product/Qiagen
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    dnase on column digestion - by Bioz Stars, 2019-12
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    Image Search Results


    Summary of  in vitro  primer extension and DNase I and KMnO 4  footprinting. ( A ) Sequence of P vpsL  from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4  footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4  cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.

    Journal: Nucleic Acids Research

    Article Title: VpsR and cyclic di-GMP together drive transcription initiation to activate biofilm formation in Vibrio cholerae

    doi: 10.1093/nar/gky606

    Figure Lengend Snippet: Summary of in vitro primer extension and DNase I and KMnO 4 footprinting. ( A ) Sequence of P vpsL from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4 footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4 cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.

    Article Snippet: Cells were harvested by centrifugation and RNA was extracted using the RNeasy kit (Qiagen), and an on-column DNase I digestion (Qiagen) was performed according to manufacturer's instructions.

    Techniques: In Vitro, Footprinting, Sequencing, Labeling, Binding Assay

    DNase I footprinting of P vpsL  complexes on ( A ) nontemplate DNA and ( B ) template DNA. GA corresponds to G+A ladder. VpsR, c-di-GMP and/or RNAP are present as indicated. To the right of each gel image, a schematic indicates the −10 and −35 regions and the +1. The VpsR binding site is indicated as a dashed black line. DNase I protection regions and hypersensitive sites seen with the activated complex of RNAP, VpsR, c-di-GMP and DNA are depicted as black rectangles and horizontal arrows, respectively. The dashed red boxes indicate the regions of DNA where the protection/enhancement within and immediately adjacent to the VpsR binding site changes when comparing complexes containing RNAP with or without VpsR or c-di-GMP to the activated complex. (See text for details.)

    Journal: Nucleic Acids Research

    Article Title: VpsR and cyclic di-GMP together drive transcription initiation to activate biofilm formation in Vibrio cholerae

    doi: 10.1093/nar/gky606

    Figure Lengend Snippet: DNase I footprinting of P vpsL complexes on ( A ) nontemplate DNA and ( B ) template DNA. GA corresponds to G+A ladder. VpsR, c-di-GMP and/or RNAP are present as indicated. To the right of each gel image, a schematic indicates the −10 and −35 regions and the +1. The VpsR binding site is indicated as a dashed black line. DNase I protection regions and hypersensitive sites seen with the activated complex of RNAP, VpsR, c-di-GMP and DNA are depicted as black rectangles and horizontal arrows, respectively. The dashed red boxes indicate the regions of DNA where the protection/enhancement within and immediately adjacent to the VpsR binding site changes when comparing complexes containing RNAP with or without VpsR or c-di-GMP to the activated complex. (See text for details.)

    Article Snippet: Cells were harvested by centrifugation and RNA was extracted using the RNeasy kit (Qiagen), and an on-column DNase I digestion (Qiagen) was performed according to manufacturer's instructions.

    Techniques: Footprinting, Binding Assay