on column dnasei digestion  (Qiagen)

 
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    Name:
    RNase Free DNase Set
    Description:
    For DNase digestion during RNA purification Kit contents Qiagen RNase free DNase Set 50 preps For DNase Digestion During RNA Purification Silica gel Membrane Spin column Technology Efficiently Removes the Majority of the DNA Without DNase Treatment The Buffer is Also Well suited for Efficient DNase Digestion in Solution Includes 1500U RNase free DNase I RNase free Buffer RDD and RNase free Water
    Catalog Number:
    79254
    Price:
    108
    Category:
    RNase Free DNase Set
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    Structured Review

    Qiagen on column dnasei digestion
    RNase Free DNase Set
    For DNase digestion during RNA purification Kit contents Qiagen RNase free DNase Set 50 preps For DNase Digestion During RNA Purification Silica gel Membrane Spin column Technology Efficiently Removes the Majority of the DNA Without DNase Treatment The Buffer is Also Well suited for Efficient DNase Digestion in Solution Includes 1500U RNase free DNase I RNase free Buffer RDD and RNase free Water
    https://www.bioz.com/result/on column dnasei digestion/product/Qiagen
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    on column dnasei digestion - by Bioz Stars, 2020-08
    99/100 stars

    Images

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Cadherin-23 Mediates Heterotypic Cell-Cell Adhesion between Breast Cancer Epithelial Cells and Fibroblasts
    Article Snippet: .. qPCR Confluent monocultures of MCF-7s and NBFs were trypsinized and ∼20×104 cells were used for mRNA isolation (RNEasy with RNAse-Free DNase set, Qiagen). qPCR was performed using the QuantiFast SYBR Green RT-PCR kit (Qiagen) on a LightCycler 480 (Roche, Indianapolis, IN). ..

    Isolation:

    Article Title: Cadherin-23 Mediates Heterotypic Cell-Cell Adhesion between Breast Cancer Epithelial Cells and Fibroblasts
    Article Snippet: .. qPCR Confluent monocultures of MCF-7s and NBFs were trypsinized and ∼20×104 cells were used for mRNA isolation (RNEasy with RNAse-Free DNase set, Qiagen). qPCR was performed using the QuantiFast SYBR Green RT-PCR kit (Qiagen) on a LightCycler 480 (Roche, Indianapolis, IN). ..

    Purification:

    Article Title: Dual-functional peptide with defective interfering genes effectively protects mice against avian and seasonal influenza
    Article Snippet: .. Extracted RNA were treated with DNase I (QIAGEN, Cat# 79254, USA) according to the manufacturer’s protocol and purified by RNeasy Mini Kit (QIAGEN, Cat# 74106, USA) to exclude plasmid DNA contamination. .. Real-time RT-qPCR was performed as we described previously .

    Article Title: Enhanced methods for unbiased deep sequencing of Lassa and Ebola RNA viruses from clinical and biological samples
    Article Snippet: .. The complementary DNA probes were removed by bringing the reaction up to 75 μL and treating with RNase-free DNase kit (Qiagen) according to the manufacturer’s protocol. rRNA-depleted samples were purified using 2.2× volumes AMPure RNA clean beads (Beckman Coulter Genomics) and eluted into 10 μL water for cDNA synthesis. .. Illumina library construction and sequencing For the experiments in this study, selectively-depleted EBOV and LASV RNA were fragmented for 4 minutes at 85° C using NEBNext Fragmentation buffer (New England Biolabs).

    SYBR Green Assay:

    Article Title: Cadherin-23 Mediates Heterotypic Cell-Cell Adhesion between Breast Cancer Epithelial Cells and Fibroblasts
    Article Snippet: .. qPCR Confluent monocultures of MCF-7s and NBFs were trypsinized and ∼20×104 cells were used for mRNA isolation (RNEasy with RNAse-Free DNase set, Qiagen). qPCR was performed using the QuantiFast SYBR Green RT-PCR kit (Qiagen) on a LightCycler 480 (Roche, Indianapolis, IN). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Cadherin-23 Mediates Heterotypic Cell-Cell Adhesion between Breast Cancer Epithelial Cells and Fibroblasts
    Article Snippet: .. qPCR Confluent monocultures of MCF-7s and NBFs were trypsinized and ∼20×104 cells were used for mRNA isolation (RNEasy with RNAse-Free DNase set, Qiagen). qPCR was performed using the QuantiFast SYBR Green RT-PCR kit (Qiagen) on a LightCycler 480 (Roche, Indianapolis, IN). ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Transformation of accessible chromatin and 3D nucleome underlies lineage commitment of early T cells
    Article Snippet: .. Total RNA was extracted and on-column digestion with DNase (QIAGEN, Cat79254) was performed, followed by elution with 10μl of RNase-free water. .. Total RNA from 1K cells was reverse transcribed by SuperScript II (Invitrogen, Cat#18064-014) with oligo-dT and LNA-containing TSO primers in a final reaction volume of 10μl using the condition: 42°C for 90min, 10 cycles of 50°C 2min to 42°C 2min, 70°C for 15min and hold at 4°C. cDNA was pre-amplified by PCR using KAPA HiFi HotStart ReadyMix (KAPABIOSYSTEMS Cat#KK2602) with IS PCR for 12 cycles in 25μl.

    Plasmid Preparation:

    Article Title: Dual-functional peptide with defective interfering genes effectively protects mice against avian and seasonal influenza
    Article Snippet: .. Extracted RNA were treated with DNase I (QIAGEN, Cat# 79254, USA) according to the manufacturer’s protocol and purified by RNeasy Mini Kit (QIAGEN, Cat# 74106, USA) to exclude plasmid DNA contamination. .. Real-time RT-qPCR was performed as we described previously .

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  • 99
    Qiagen on column dnase i digestion
    Summary of in vitro primer extension and <t>DNase</t> I and KMnO 4 footprinting. ( A ) Sequence of P vpsL from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4 footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4 cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.
    On Column Dnase I Digestion, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/on column dnase i digestion/product/Qiagen
    Average 99 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    on column dnase i digestion - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    90
    Qiagen on column dnase i digestion step
    <t>DNase</t> I footprinting assay of EsaR binding sites in the noncoding strand of P dkgA , coding strand of P glpF , and noncoding strand of P lrhA . (A to C) Capillary electrophoresis of FAM-labeled DNA fragments P dkgA (A), P glpF (B), and P lrhA (C) from DNase I
    On Column Dnase I Digestion Step, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/on column dnase i digestion step/product/Qiagen
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    on column dnase i digestion step - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    Image Search Results


    Summary of in vitro primer extension and DNase I and KMnO 4 footprinting. ( A ) Sequence of P vpsL from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4 footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4 cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.

    Journal: Nucleic Acids Research

    Article Title: VpsR and cyclic di-GMP together drive transcription initiation to activate biofilm formation in Vibrio cholerae

    doi: 10.1093/nar/gky606

    Figure Lengend Snippet: Summary of in vitro primer extension and DNase I and KMnO 4 footprinting. ( A ) Sequence of P vpsL from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4 footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4 cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.

    Article Snippet: Cells were harvested by centrifugation and RNA was extracted using the RNeasy kit (Qiagen), and an on-column DNase I digestion (Qiagen) was performed according to manufacturer's instructions.

    Techniques: In Vitro, Footprinting, Sequencing, Labeling, Binding Assay

    DNase I footprinting of P vpsL complexes on ( A ) nontemplate DNA and ( B ) template DNA. GA corresponds to G+A ladder. VpsR, c-di-GMP and/or RNAP are present as indicated. To the right of each gel image, a schematic indicates the −10 and −35 regions and the +1. The VpsR binding site is indicated as a dashed black line. DNase I protection regions and hypersensitive sites seen with the activated complex of RNAP, VpsR, c-di-GMP and DNA are depicted as black rectangles and horizontal arrows, respectively. The dashed red boxes indicate the regions of DNA where the protection/enhancement within and immediately adjacent to the VpsR binding site changes when comparing complexes containing RNAP with or without VpsR or c-di-GMP to the activated complex. (See text for details.)

    Journal: Nucleic Acids Research

    Article Title: VpsR and cyclic di-GMP together drive transcription initiation to activate biofilm formation in Vibrio cholerae

    doi: 10.1093/nar/gky606

    Figure Lengend Snippet: DNase I footprinting of P vpsL complexes on ( A ) nontemplate DNA and ( B ) template DNA. GA corresponds to G+A ladder. VpsR, c-di-GMP and/or RNAP are present as indicated. To the right of each gel image, a schematic indicates the −10 and −35 regions and the +1. The VpsR binding site is indicated as a dashed black line. DNase I protection regions and hypersensitive sites seen with the activated complex of RNAP, VpsR, c-di-GMP and DNA are depicted as black rectangles and horizontal arrows, respectively. The dashed red boxes indicate the regions of DNA where the protection/enhancement within and immediately adjacent to the VpsR binding site changes when comparing complexes containing RNAP with or without VpsR or c-di-GMP to the activated complex. (See text for details.)

    Article Snippet: Cells were harvested by centrifugation and RNA was extracted using the RNeasy kit (Qiagen), and an on-column DNase I digestion (Qiagen) was performed according to manufacturer's instructions.

    Techniques: Footprinting, Binding Assay

    Summary of in vitro primer extension and DNase I and KMnO 4 footprinting. ( A ) Sequence of P vpsL from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4 footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4 cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.

    Journal: Nucleic Acids Research

    Article Title: VpsR and cyclic di-GMP together drive transcription initiation to activate biofilm formation in Vibrio cholerae

    doi: 10.1093/nar/gky606

    Figure Lengend Snippet: Summary of in vitro primer extension and DNase I and KMnO 4 footprinting. ( A ) Sequence of P vpsL from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4 footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4 cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.

    Article Snippet: Cells were harvested by centrifugation and RNA was extracted using the RNeasy kit (Qiagen), and an on-column DNase I digestion (Qiagen) was performed according to manufacturer's instructions.

    Techniques: In Vitro, Footprinting, Sequencing, Labeling, Binding Assay

    DNase I footprinting of P vpsL complexes on ( A ) nontemplate DNA and ( B ) template DNA. GA corresponds to G+A ladder. VpsR, c-di-GMP and/or RNAP are present as indicated. To the right of each gel image, a schematic indicates the −10 and −35 regions and the +1. The VpsR binding site is indicated as a dashed black line. DNase I protection regions and hypersensitive sites seen with the activated complex of RNAP, VpsR, c-di-GMP and DNA are depicted as black rectangles and horizontal arrows, respectively. The dashed red boxes indicate the regions of DNA where the protection/enhancement within and immediately adjacent to the VpsR binding site changes when comparing complexes containing RNAP with or without VpsR or c-di-GMP to the activated complex. (See text for details.)

    Journal: Nucleic Acids Research

    Article Title: VpsR and cyclic di-GMP together drive transcription initiation to activate biofilm formation in Vibrio cholerae

    doi: 10.1093/nar/gky606

    Figure Lengend Snippet: DNase I footprinting of P vpsL complexes on ( A ) nontemplate DNA and ( B ) template DNA. GA corresponds to G+A ladder. VpsR, c-di-GMP and/or RNAP are present as indicated. To the right of each gel image, a schematic indicates the −10 and −35 regions and the +1. The VpsR binding site is indicated as a dashed black line. DNase I protection regions and hypersensitive sites seen with the activated complex of RNAP, VpsR, c-di-GMP and DNA are depicted as black rectangles and horizontal arrows, respectively. The dashed red boxes indicate the regions of DNA where the protection/enhancement within and immediately adjacent to the VpsR binding site changes when comparing complexes containing RNAP with or without VpsR or c-di-GMP to the activated complex. (See text for details.)

    Article Snippet: Cells were harvested by centrifugation and RNA was extracted using the RNeasy kit (Qiagen), and an on-column DNase I digestion (Qiagen) was performed according to manufacturer's instructions.

    Techniques: Footprinting, Binding Assay

    DNase I footprinting analysis of N. gonorrhoeae Fur protein with gonococcal fur and tonB promoter/operator regions. (A) DNase I footprint analysis of the fur promoter operator region. Radiolabeled DNA was incubated with increasing concentrations of Fur

    Journal: Journal of Bacteriology

    Article Title: Expression of the Gonococcal Global Regulatory Protein Fur and Genes Encompassing the Fur and Iron Regulon during In Vitro and In Vivo Infection in Women

    doi: 10.1128/JB.01830-07

    Figure Lengend Snippet: DNase I footprinting analysis of N. gonorrhoeae Fur protein with gonococcal fur and tonB promoter/operator regions. (A) DNase I footprint analysis of the fur promoter operator region. Radiolabeled DNA was incubated with increasing concentrations of Fur

    Article Snippet: On-column DNase I digestion was also performed for each sample according to the manufacturer's instructions (Qiagen).

    Techniques: Footprinting, Incubation

    DNase I footprinting assay of EsaR binding sites in the noncoding strand of P dkgA , coding strand of P glpF , and noncoding strand of P lrhA . (A to C) Capillary electrophoresis of FAM-labeled DNA fragments P dkgA (A), P glpF (B), and P lrhA (C) from DNase I

    Journal: Applied and Environmental Microbiology

    Article Title: Proteomic Analysis of the Quorum-Sensing Regulon in Pantoea stewartii and Identification of Direct Targets of EsaR

    doi: 10.1128/AEM.01744-13

    Figure Lengend Snippet: DNase I footprinting assay of EsaR binding sites in the noncoding strand of P dkgA , coding strand of P glpF , and noncoding strand of P lrhA . (A to C) Capillary electrophoresis of FAM-labeled DNA fragments P dkgA (A), P glpF (B), and P lrhA (C) from DNase I

    Article Snippet: The cell pellets were stored at −80°C prior to total RNA extraction using the RNeasy minikit (Qiagen) with an additional on-column DNase I digestion step using the RNase-free DNase set (Qiagen).

    Techniques: Footprinting, Binding Assay, Electrophoresis, Labeling

    Nested deletion EMSA analysis of EsaR direct targets. (A to C) The region protected by DNase I digestion in P dkgA (A), P glpF (B), and P lrhA (C) is the gray-shaded sequence (5′ to 3′), and the underlined bases are the 20-bp EsaR binding

    Journal: Applied and Environmental Microbiology

    Article Title: Proteomic Analysis of the Quorum-Sensing Regulon in Pantoea stewartii and Identification of Direct Targets of EsaR

    doi: 10.1128/AEM.01744-13

    Figure Lengend Snippet: Nested deletion EMSA analysis of EsaR direct targets. (A to C) The region protected by DNase I digestion in P dkgA (A), P glpF (B), and P lrhA (C) is the gray-shaded sequence (5′ to 3′), and the underlined bases are the 20-bp EsaR binding

    Article Snippet: The cell pellets were stored at −80°C prior to total RNA extraction using the RNeasy minikit (Qiagen) with an additional on-column DNase I digestion step using the RNase-free DNase set (Qiagen).

    Techniques: Sequencing, Binding Assay