on column dnase digestion  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Qiagen on column dnase digestion
    On Column Dnase Digestion, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 242 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/on column dnase digestion/product/Qiagen
    Average 99 stars, based on 242 article reviews
    Price from $9.99 to $1999.99
    on column dnase digestion - by Bioz Stars, 2020-01
    99/100 stars

    Images

    Related Articles

    other:

    Article Title:
    Article Snippet: The aqueous phase of the phenol–chloroform extraction was further purified using the RNeasy Mini kit (Qiagen) and on-column DNase digestion using the RNase free DNase kit (Qiagen).

    Article Title:
    Article Snippet: Transcriptome Analysis BEAS-2B cells were either left untreated or treated with 0.2 ng/ml of IFNβ and 5 ng/ml of IFNλ1 for 24 h and RNA was isolated using RNeasy spin columns including on-column DNase digestion (Qiagen,Germantown, MD).

    Article Title:
    Article Snippet: RNA isolation and transcriptome analysis Synchronized populations of fer-15;fem-1 mutants were harvested to examine gene expression in the host in response to preconditioning with JDFM216 or OP50 for 24 h. Total RNA from worms was immediately isolated using TRIZOL reagent (Invitrogen), according to the manufacturer’s instructions, and purified using an RNeasy Mini Kit (Qiagen) including an on-column DNase digestion with RNase-free DNase (Qiagen) coupled with a mini bead beater (Biospec, Bartlesville, OK).

    Article Title:
    Article Snippet: Total RNA was extracted using the RNeasy Mini kit (QIAGEN) performing on-column DNase digestion with RNase-Free DNase Set (QIAGEN), according to the manufacturer's instructions.

    Article Title:
    Article Snippet: Total RNA was extracted using the RNeasy mini kit with on-column DNase digestion (Qiagen).

    Article Title:
    Article Snippet: RNA was prepared from siRNA transfected HCT116 cultures at the indicated time points post-siRNA transfection using the RNeasy Mini Kit (Qiagen cat. # 74104) including on-column DNase digestion (Qiagen cat. # 79254) and eluted in the supplied RNase-free water.

    Article Title:
    Article Snippet: For quantitative RT-PCR analysis, total RNA was extracted from around 5 × 105 cells using an RNeasy Mini Kit (Qiagen) with on-column DNase digestion using an RNase-Free DNase Set (Qiagen), according to the manufacturer’s instructions.

    Article Title:
    Article Snippet: RNA was extracted from 20 mg of frozen material using RNeasy Plant kit (Qiagen) combined with on-column DNase digestion using RNase-Free DNase Set (Qiagen).

    Article Title:
    Article Snippet: Aqueous chloroform fractions were mixed with equal volumes of 70% ethanol/30% water and RNA further purified with RNeasy Mini spin columns (Qiagen) and on-column DNase-digestion (Qiagen).

    Article Title:
    Article Snippet: Cells were then immediately harvested and RNA was isolated and column purified using the RNeasy Mini Kit with on-column DNase digestion (Qiagen, Hilden, Germany; Cat. No. 74106).

    Article Title:
    Article Snippet: Prior to isolating RNA using an RNeasy minikit (Qiagen) with on-column DNase digestion, samples were digested with Proteinase K (Qiagen) for 10 minutes at 55°C.

    Article Title:
    Article Snippet: Total cellular RNA was harvested with the RNeasy Mini Kit, followed by on-column DNAse digestion (Qiagen, Hilden, Germany).

    Article Title:
    Article Snippet: Total RNA was extracted using the RNeasy Mini Kit (Qiagen) performing on-column DNase digestion with RNase-Free DNase Set (Qiagen) according to the manufacturer's instructions.

    Article Title:
    Article Snippet: RNA isolation, cDNA synthesis, and real-time PCR Total RNA from cultured cells was isolated 24 h after indicated treatments and purified with on-column DNase digestion using miRNeasy Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s protocol.

    Article Title:
    Article Snippet: On-column DNase digestion (QIAGEN) were performed for all samples during RNA extraction according to manufacturer’s protocol.

    Article Title:
    Article Snippet: Total RNA from tissue lysates was purified by RNeasy mini kit (Qiagen) and on-column DNase digestion using an RNase-Free DNase Set (Qiagen).

    Article Title:
    Article Snippet: Total RNA was extracted and purified from whole-cell lysates using the RNeasy Mini Kit with on-column DNase digestion (Qiagen, Hilden, Germany; Cat. No. 74106).

    Article Title:
    Article Snippet: To purify RNA, stabilized cells were processed by using the RNeasy plant minikit (Qiagen) and on-column DNase digestion with the RNase-free DNase set (Qiagen).

    Article Title:
    Article Snippet: RNA was isolated using RNeasy with DNase on-column digestion (Qiagen).

    Article Title:
    Article Snippet: Total RNA from homogenized tissue was isolated and purified using an RNeasy mini kit with on-column DNase digestion (Qiagen) and kept at -80°C.

    Article Title:
    Article Snippet: After collection of the nematodes, total RNA was quickly isolated following the protocol of the TRIzol reagent (Invitrogen) and purified using the RNeasy minikit (Qiagen) including an on-column DNase digestion with RNase-free DNase (Qiagen).

    Article Title:
    Article Snippet: Cells were harvested at 24 hours for qPCR analysis with RNA isolated using the RNeasy Plus Mini kit with on-column Dnase digestion per manufacturer’s instructions (Qiagen).

    Article Title:
    Article Snippet: Real-time PCR Total RNA extraction was performed with RNeasy Mini Kit (Qiagen) including On-column DNase Digestion with RNase-Free DNase set (Qiagen).

    Article Title:
    Article Snippet: Total cellular RNA from each replicate was isolated using TRIZOL (Invitrogen) and purified using RNeasy Mini Kit (Qiagen) with on-column DNase digestion performed with RNase-free DNase Set (Qiagen).

    Article Title:
    Article Snippet: RNA isolation, cDNA preparation, and RT-qPCR Total RNA was extracted and purified from whole-cell lysates using the RNeasy Mini Kit with on-column DNase digestion (Qiagen, Hilden, Germany; Cat. No. 74106).

    Article Title:
    Article Snippet: To remove genomic DNA, On-Column DNase Digestion was performed using an RNase-Free DNase Set (QIAGEN).

    Article Title:
    Article Snippet: RNA isolation and quantitative Real-Time PCR (qRT-PCR) Total RNA was isolated by RNeasy Mini kit (Qiagen ) including on-column DNase digestion to eliminate DNA (Rnase-Free DNase Set, Qiagen).

    Article Title:
    Article Snippet: Total RNA was isolated from epithelial cells using RNeasy reagent (Qiagen, Valencia, CA) and QIAshredder columns according to the manufacturer’s recommendations (Qiagen), and purified by elution through RNeasy columns (Qiagen) with on-column DNase digestion using the RNase-Free DNase set (Qiagen).

    Article Title:
    Article Snippet: Total RNA was extracted using an RNeasy minikit (Qiagen) with on-column DNase digestion using the RNase-free DNase (Qiagen) according to the supplier's instructions.

    Article Title:
    Article Snippet: Reverse transcription (RT)-PCR Total RNA was extracted using the RNeasy mini kit with on-column DNase digestion (Qiagen).

    Article Title:
    Article Snippet: RNA was extracted using TRI reagent (Sigma) and then subjected to a cleanup step using an RNeasy Mini Kit with on-column DNase digestion (Qiagen).

    Article Title:
    Article Snippet: Approximately 15 flies were homogenized in TRIzol reagent (Invitrogen) to isolate total RNA, which was then purified with an RNeasy minikit (Qiagen) with on-column DNase digestion. cDNA was synthesized from 1 μg of the purified RNA with a QuantiTect (Qiagen) reverse transcription kit.

    Article Title:
    Article Snippet: Synchronized populations of fer-15;fem-1 mutants were harvested to examine gene expression in the host in response to preconditioning with JDFM216 or OP50 for 24 h. Total RNA from worms was immediately isolated using TRIZOL reagent (Invitrogen), according to the manufacturer’s instructions, and purified using an RNeasy Mini Kit (Qiagen) including an on-column DNase digestion with RNase-free DNase (Qiagen) coupled with a mini bead beater (Biospec, Bartlesville, OK).

    Article Title:
    Article Snippet: Colonic RNA was extracted with TRIzol reagent (Invitrogen) and cleaned on RNeasy Mini columns (Qiagen) with on-column DNase digestion performed using the RNAse-Free DNase kit (Qiagen) according to manufacturer's instructions.

    Article Title:
    Article Snippet: Gene expression analysis of tendon markers Total RNA was isolated from MSC monolayer cultures (P3) using the RNeasy Mini Kit with On-Column DNase digestion (both Qiagen, Hilden, Germany).

    Article Title:
    Article Snippet: Total RNA was isolated from cells using RNeasy reagent (Qiagen, Valencia, CA) and QIAshredder columns according to the manufacturer's recommendations (Qiagen), and purified on RNeasy columns (Qiagen) with on-column DNase digestion using the RNase-Free DNase set (Qiagen).

    Article Title:
    Article Snippet: RNA extraction and real-time quantitative PCR (RT-qPCR) Total RNA was isolated from breast tissue samples using Tissue Lyser (Qiagen, Hilden, Germany) and RNeasy Lipid Tissue Mini Kit (Qiagen) and the on-column DNase digestion with RNase-free DNase Set (Qiagen) following the manufacturers’ instructions.

    Article Title:
    Article Snippet: Total RNA was purified using the RNeasy Maxi kit (Qiagen) with on-column DNase digestion, and 500 μg was used as input for the Oligotex mRNA Midi kit (Qiagen), yielding ~3% mRNA.

    Article Title:
    Article Snippet: Total RNA was extracted using the RNeasy Mini Kit (Qiagen) performing on-column DNase digestion with RNase-Free DNase Set (Qiagen), according to the manufacturer’s instructions.

    Article Title:
    Article Snippet: Real-time RT-PCR of TLR mRNAs in dMs and dNK cells Total RNA was extracted from purified dM and dNK cells and from decidual mononuclear cells by using the RNeasy Mini kit (Qiagen) and on-column DNase digestion using the RNase-free DNase set (Qiagen).

    Article Title:
    Article Snippet: RT and qPCR Total cellular RNA was harvested with the RNeasy Mini Kit, followed by on-column DNAse digestion (Qiagen, Hilden, Germany).

    Article Title:
    Article Snippet: Quantitative RT-PCR For quantitative RT-PCR analysis, total RNA was extracted from around 5 × 105 cells using an RNeasy Mini Kit (Qiagen) with on-column DNase digestion using an RNase-Free DNase Set (Qiagen), according to the manufacturer’s instructions.

    Article Title:
    Article Snippet: An aliquot of 20 μg of total RNA was further purified using the RNeasy mini kit (Qiagen) with on-column DNase digestion to remove any trace of genomic DNA contamination. cDNA was obtained by reverse transcription of 1 μg of purified RNA using the Omniscript RT kit (Qiagen) and analyzed by quantitative real-time PCR using primer pairs as shown in Additional file .

    Article Title:
    Article Snippet: Prior to performing standard RNA extraction protocols (with on-column DNase digestion) with RNeasy min kits (Qiagen), cells were enzymatically treated with 100μL 2U/μL lyticase solution (Sigma #L2524) for 10min at 30°C.

    Article Title:
    Article Snippet: RNA isolation and microarray hybridisation Total RNA was isolated using the RNeasy® Mini Kit (QIAGEN AG) and on-column DNase digestion was performed using RNase-Free DNase Set (QIAGEN AG).

    Article Title:
    Article Snippet: Total RNA from worms was quickly isolated following the protocol of the TRIzol reagent (Invitrogen) and purified using the RNeasy minikit (Qiagen) including an on-column DNase digestion with RNase-free DNase (Qiagen).

    Article Title:
    Article Snippet: RNA was extracted using the RNeasy plant mini kit (Qiagen); on-column DNase digestion with the RNase-Free DNase set (Qiagen) was performed to remove contaminating DNA.

    Article Title:
    Article Snippet: RNA purification was performed using the RNEasy kit with on-column DNAse digestion (Qiagen, Germantown MD).

    Article Title:
    Article Snippet: RNA was purified using the RNeasy minikit (Qiagen) with on-column DNase digestion using the RNase-free DNase set (Qiagen) and then reverse-transcribed using the Transcriptor first strand cDNA synthesis kit (Roche) using 60 μM random hexamer primers.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Qiagen rnase free dnase set
    Rnase Free Dnase Set, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 962 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase free dnase set/product/Qiagen
    Average 90 stars, based on 962 article reviews
    Price from $9.99 to $1999.99
    rnase free dnase set - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results