on column dnase digestion step the rnase free dnase set qiagen  (Qiagen)


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    Name:
    RNase Free DNase Set
    Description:
    For DNase digestion during RNA purification Kit contents Qiagen RNase free DNase Set 50 preps For DNase Digestion During RNA Purification Silica gel Membrane Spin column Technology Efficiently Removes the Majority of the DNA Without DNase Treatment The Buffer is Also Well suited for Efficient DNase Digestion in Solution Includes 1500U RNase free DNase I RNase free Buffer RDD and RNase free Water
    Catalog Number:
    79254
    Price:
    108
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    RNase Free DNase Set
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    Qiagen on column dnase digestion step the rnase free dnase set qiagen
    RNase Free DNase Set
    For DNase digestion during RNA purification Kit contents Qiagen RNase free DNase Set 50 preps For DNase Digestion During RNA Purification Silica gel Membrane Spin column Technology Efficiently Removes the Majority of the DNA Without DNase Treatment The Buffer is Also Well suited for Efficient DNase Digestion in Solution Includes 1500U RNase free DNase I RNase free Buffer RDD and RNase free Water
    https://www.bioz.com/result/on column dnase digestion step the rnase free dnase set qiagen/product/Qiagen
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    on column dnase digestion step the rnase free dnase set qiagen - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Synthesized:

    Article Title: The effect of cellobiose on the health status of growing rabbits depends on the dietary level of soluble fiber
    Article Snippet: A DNAse treatment step using RNase-Free DNase Set (Qiagen, Hilden, Germany) was added to prevent genomic DNA contamination. .. First strand cDNA was synthesized using the High-Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA) according to the manufacturer’s instructions.

    Quantitative RT-PCR:

    Article Title: Smooth muscle cell-specific FoxM1 controls hypoxia-induced pulmonary hypertension
    Article Snippet: Paragraph title: Quantitative Real-time Reverse Transcription PCR (qRT-PCR) ... We used a miRNeasy Mini Kit (Qiagen, Valencia, CA) and an RNase-Free DNase Set (Qiagen) to extract the total RNA.

    Article Title: Proteomic analysis of Salmonella enterica serovar Enteritidis following propionate adaptation
    Article Snippet: Quantitative Real Time PCR Five proteins overexpressed in PA adapted 2 D gels were selected for further study to monitor changes at the mRNA level using quantitative real time PCR (qRT-PCR). .. Contaminating genomic DNA was removed from the RNA sample using the RNase-Free DNase set (Qiagen).

    Article Title: Engineering d‐limonene synthase down‐regulation in orange fruit induces resistance against the fungus Phyllosticta citricarpa through enhanced accumulation of monoterpene alcohols and activation of defence
    Article Snippet: .. RNA was treated with DNase I (RNase‐Free DNase Set; Qiagen) for real‐time qRT‐PCR analysis and quantified using a NanoDrop spectrophotometer (NanoDrop 2000C; Thermo Scientific, USA). .. Microarray assays were performed with the flavedo of two independent AS (AS1 and AS7) and EV transgenic ‘Navelina’ mature orange fruit, inoculated with P. citricarpa , comparing randomly selected AS vs. EV samples on the same slide with three biological replicates for each genotype.

    SYBR Green Assay:

    Article Title: Smooth muscle cell-specific FoxM1 controls hypoxia-induced pulmonary hypertension
    Article Snippet: We used a miRNeasy Mini Kit (Qiagen, Valencia, CA) and an RNase-Free DNase Set (Qiagen) to extract the total RNA. .. We determined the quantity and quality of total RNA with Nanodrop 2000 spectrophotometer (ThermoScientific, Rockford, IL) and then performed reverse transcription using High Capacity Reverse Transcription kits (Applied Biosystems, Foster City, CA). qRT-PCR was conducted with SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA) on a StepOnePlus or a ViiA 7 Real-Time PCR System (Applied Biosystems).

    Microarray:

    Article Title: Cancer Immunoediting from Immunosurveillance to Tumor Escape in Microvillus-Formed Niche: A Study of Syngeneic Orthotopic Rat Bladder Cancer Model in Comparison with Human Bladder Cancer 1
    Article Snippet: Paragraph title: Complementary DNA Microarray and Data Analysis ... Cultured AY-27 cells and the entire bladder from each rat were taken and immediately frozen in a bath of liquid nitrogen and stored at -80°C until isolation of RNA using the RNeasy Fibrous Midi Kit and RNase-free DNase Set (Qiagen, Valencia, CA).

    Incubation:

    Article Title: Time-resolved mapping of genetic interactions to model rewiring of signaling pathways
    Article Snippet: After 72 hr incubation (25°C, no CO2 adjustment), cells were washed once in 750 µl PBS (Gibco) and lysed in 350 µl RLT buffer shipped with the RNAeasy-mini Kit (Qiagen). .. An optional DNase digestion step was performed using the RNase-Free DNase Set (Qiagen).

    Activity Assay:

    Article Title: The effect of cellobiose on the health status of growing rabbits depends on the dietary level of soluble fiber
    Article Snippet: Paragraph title: Ileal and Cecal Fermentation, Sucrose Activity, and Immune Response Trial ... A DNAse treatment step using RNase-Free DNase Set (Qiagen, Hilden, Germany) was added to prevent genomic DNA contamination.

    Expressing:

    Article Title: A neurotoxic regimen of methamphetamine exacerbates the febrile and neuroinflammatory response to a subsequent peripheral immune stimulus
    Article Snippet: DNase treatment was performed on a PicoPure column with a Qiagen RNase-free DNase set (Qiagen, Valenica, CA). .. Quantitative real-time PCR was performed using the Applied Biosystems (Foster, CA) Assay-on Demand Gene Expression protocol as previously described [ ].

    Article Title: Germination and the Early Stages of Seedling Development in Brachypodium distachyon
    Article Snippet: Paragraph title: 4.4. Gene Expression Analysis ... The RNA was purified using Qiagen RNase-Free DNase set (Qiagen, Venlo, Netherlands) and an RNeasy MinElute Cleanup Kit (Qiagen, Venlo, Netherlands).

    Derivative Assay:

    Article Title: Pulsed electromagnetic fields increase osteogenetic commitment of MSCs via the mTOR pathway in TNF-α mediated inflammatory conditions: an in-vitro study
    Article Snippet: .. RNase-Free DNase Set (Qiagen) from implants were cultured with adipose tissue derived mesenchymal stem cells for 15 and 25 days. .. The RNA quality and concentration of the samples were measured using a NanoDropTM ND-1000 Spectrophotometer (Thermo Scientific).

    Transfection:

    Article Title: Time-resolved mapping of genetic interactions to model rewiring of signaling pathways
    Article Snippet: To this end, as 5*105 cells / well were seeded in 630 µl ExpressFive (Gibco) culture medium and reverse transfected with 14 µg dsRNA. .. An optional DNase digestion step was performed using the RNase-Free DNase Set (Qiagen).

    Cell Culture:

    Article Title: Cancer Immunoediting from Immunosurveillance to Tumor Escape in Microvillus-Formed Niche: A Study of Syngeneic Orthotopic Rat Bladder Cancer Model in Comparison with Human Bladder Cancer 1
    Article Snippet: .. Cultured AY-27 cells and the entire bladder from each rat were taken and immediately frozen in a bath of liquid nitrogen and stored at -80°C until isolation of RNA using the RNeasy Fibrous Midi Kit and RNase-free DNase Set (Qiagen, Valencia, CA). .. Isolated RNA was analyzed by Affymetrix GeneChip (Affymetrix, Santa Clara, CA) with a RAE 230 A chip that includes more than 15,000 rat genes.

    Article Title: Pulsed electromagnetic fields increase osteogenetic commitment of MSCs via the mTOR pathway in TNF-α mediated inflammatory conditions: an in-vitro study
    Article Snippet: .. RNase-Free DNase Set (Qiagen) from implants were cultured with adipose tissue derived mesenchymal stem cells for 15 and 25 days. .. The RNA quality and concentration of the samples were measured using a NanoDropTM ND-1000 Spectrophotometer (Thermo Scientific).

    other:

    Article Title: Stress-Independent Activation of XBP1s and/or ATF6 Reveals Three Functionally Diverse ER Proteostasis Environments
    Article Snippet: Genomic DNA was removed by on-column digestion using the RNase-free DNase Set (QIAGEN).

    Sequencing:

    Article Title: Circ0043898 acts as a tumor inhibitor and performs regulatory effect on the inhibition of esophageal carcinoma
    Article Snippet: RNA Clean XP Kit (Beckman Coulter, CA, UA) and RNase-free DNase Set (QIAGEN, Germany) were used for RNA cleanup. .. For circRNA sequencing, total RNA was digested with RNase R (Epicenter, USA) in order to remove the linear RNAs.

    Nucleic Acid Electrophoresis:

    Article Title: Early life antibiotic exposure affects pancreatic islet development and metabolic regulation
    Article Snippet: On column DNase (RNase-free DNase set; QIAGEN) was applied to eliminate genomic DNA contamination in the RNA samples. .. RNA concentrations and purity were determined by a NanoDrop 2000c (Thermo Scientific) RNA quality was further verified with gel electrophoresis (RNA loading dye, Thermo Scientific) .

    RNA Sequencing Assay:

    Article Title: Circ0043898 acts as a tumor inhibitor and performs regulatory effect on the inhibition of esophageal carcinoma
    Article Snippet: Paragraph title: RNA-seq ... RNA Clean XP Kit (Beckman Coulter, CA, UA) and RNase-free DNase Set (QIAGEN, Germany) were used for RNA cleanup.

    Fluorescence:

    Article Title: Time-resolved mapping of genetic interactions to model rewiring of signaling pathways
    Article Snippet: An optional DNase digestion step was performed using the RNase-Free DNase Set (Qiagen). .. The plate was then centrifuged (2 min, 2000 rpm) and processed for qPCR in a Roche 480 LightCycler using the following PCR program: (i) 10 min at 95°C, (ii) 15 s at 95°C, (iii) 60 s at 60°C, repeat step ii) and iii) 40 times and measure fluorescence at 494 nm-521 nm during step iii).

    Isolation:

    Article Title: Early life antibiotic exposure affects pancreatic islet development and metabolic regulation
    Article Snippet: Paragraph title: RNA isolation and cDNA synthesis ... On column DNase (RNase-free DNase set; QIAGEN) was applied to eliminate genomic DNA contamination in the RNA samples.

    Article Title: Sexually Dimorphic Actions of Glucocorticoids Provide a Link to Inflammatory Diseases with Gender Differences in Prevalence
    Article Snippet: Paragraph title: RNA isolation ... All samples were treated with the RNase-Free DNase Set (Qiagen).

    Article Title: Genome-Wide Association Mapping in a Rice MAGIC Plus Population Detects QTLs and Genes Useful for Biofortification
    Article Snippet: .. Total RNA was isolated from grains using PureLink Plant RNA Reagent (Life Technologies, CA, United States) and purified using RNeasy Mini Kit with on-column DNAse treatment using RNase-Free DNase Set (Qiagen, CA, United States) as described by the manufacturers. .. RNA was eluted in 30 μl DEPC-treated water, quantified using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, DE, United States), and integrity-checked on agarose gel.

    Article Title: Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots
    Article Snippet: Cells were counted after sorting using a hemocytometer. mRNA was extracted and purified using the PicoPure RNA Isolation Kit (Thermo Fisher Scientific, Grand Island, NY, USA) according to the manufacturer's protocol ( https://www.thermofisher.com/order/catalog/product/KIT0204 ). .. The optional DNase treatment was performed according to the protocol using the RNase-Free DNase Set (Qiagen).

    Article Title: A neurotoxic regimen of methamphetamine exacerbates the febrile and neuroinflammatory response to a subsequent peripheral immune stimulus
    Article Snippet: Cytokine mRNA measurement by quantitative real-time PCR Total hypothalamic RNA was isolated using the Arcturus PicoPure™RNA isolation kit as described by the manufacturer. .. DNase treatment was performed on a PicoPure column with a Qiagen RNase-free DNase set (Qiagen, Valenica, CA).

    Article Title: The effect of cellobiose on the health status of growing rabbits depends on the dietary level of soluble fiber
    Article Snippet: Total RNA was isolated using the GenElute Mammalian Total RNA Miniprep kit (Sigma-Aldrich, St Louis, MO, USA) according to manufacturer’s instructions. .. A DNAse treatment step using RNase-Free DNase Set (Qiagen, Hilden, Germany) was added to prevent genomic DNA contamination.

    Article Title: Proteomic analysis of Salmonella enterica serovar Enteritidis following propionate adaptation
    Article Snippet: Total RNA isolation of PA adapted cells and unadapted cells was achieved using Qiagen's RNeasy® Mini Kit following the manufacturer's instructions. .. Contaminating genomic DNA was removed from the RNA sample using the RNase-Free DNase set (Qiagen).

    Article Title: Germination and the Early Stages of Seedling Development in Brachypodium distachyon
    Article Snippet: Gene Expression Analysis RNA was isolated from dry seeds (0 HAI) as well as from the developing embryos/seedlings at 4, 12, and 24 HAI. .. The RNA was purified using Qiagen RNase-Free DNase set (Qiagen, Venlo, Netherlands) and an RNeasy MinElute Cleanup Kit (Qiagen, Venlo, Netherlands).

    Article Title: Cancer Immunoediting from Immunosurveillance to Tumor Escape in Microvillus-Formed Niche: A Study of Syngeneic Orthotopic Rat Bladder Cancer Model in Comparison with Human Bladder Cancer 1
    Article Snippet: .. Cultured AY-27 cells and the entire bladder from each rat were taken and immediately frozen in a bath of liquid nitrogen and stored at -80°C until isolation of RNA using the RNeasy Fibrous Midi Kit and RNase-free DNase Set (Qiagen, Valencia, CA). .. Isolated RNA was analyzed by Affymetrix GeneChip (Affymetrix, Santa Clara, CA) with a RAE 230 A chip that includes more than 15,000 rat genes.

    Purification:

    Article Title: Early life antibiotic exposure affects pancreatic islet development and metabolic regulation
    Article Snippet: RNA isolation and cDNA synthesis Total RNA was extracted from the pancreas, ileum and proximal colon using the GeneJET RNA Purification Kit (Thermo Scientific). .. On column DNase (RNase-free DNase set; QIAGEN) was applied to eliminate genomic DNA contamination in the RNA samples.

    Article Title: Genome-Wide Association Mapping in a Rice MAGIC Plus Population Detects QTLs and Genes Useful for Biofortification
    Article Snippet: .. Total RNA was isolated from grains using PureLink Plant RNA Reagent (Life Technologies, CA, United States) and purified using RNeasy Mini Kit with on-column DNAse treatment using RNase-Free DNase Set (Qiagen, CA, United States) as described by the manufacturers. .. RNA was eluted in 30 μl DEPC-treated water, quantified using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, DE, United States), and integrity-checked on agarose gel.

    Article Title: Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots
    Article Snippet: Cells were counted after sorting using a hemocytometer. mRNA was extracted and purified using the PicoPure RNA Isolation Kit (Thermo Fisher Scientific, Grand Island, NY, USA) according to the manufacturer's protocol ( https://www.thermofisher.com/order/catalog/product/KIT0204 ). .. The optional DNase treatment was performed according to the protocol using the RNase-Free DNase Set (Qiagen).

    Article Title: Germination and the Early Stages of Seedling Development in Brachypodium distachyon
    Article Snippet: .. The RNA was purified using Qiagen RNase-Free DNase set (Qiagen, Venlo, Netherlands) and an RNeasy MinElute Cleanup Kit (Qiagen, Venlo, Netherlands). .. The concentration and quality of the isolated RNA was evaluated using an ND-1000 NanoDrop spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    Article Title: Time-resolved mapping of genetic interactions to model rewiring of signaling pathways
    Article Snippet: RNA was then purified from all samples according to manufacturer's standard instructions for spin column purification. .. An optional DNase digestion step was performed using the RNase-Free DNase Set (Qiagen).

    Polymerase Chain Reaction:

    Article Title: Genome-Wide Association Mapping in a Rice MAGIC Plus Population Detects QTLs and Genes Useful for Biofortification
    Article Snippet: Total RNA was isolated from grains using PureLink Plant RNA Reagent (Life Technologies, CA, United States) and purified using RNeasy Mini Kit with on-column DNAse treatment using RNase-Free DNase Set (Qiagen, CA, United States) as described by the manufacturers. .. Reverse transcription was done with 1 μg total RNA, 1 μl Oligo DT, 1 μl anchored-oligo primer from Transcriptor First Strand cDNA Synthesis Kit (Roche Applied Science, Mannheim, Germany), and PCR-grade water to make a total volume of 13 μl.

    Article Title: Smooth muscle cell-specific FoxM1 controls hypoxia-induced pulmonary hypertension
    Article Snippet: Paragraph title: Quantitative Real-time Reverse Transcription PCR (qRT-PCR) ... We used a miRNeasy Mini Kit (Qiagen, Valencia, CA) and an RNase-Free DNase Set (Qiagen) to extract the total RNA.

    Article Title: Proteomic analysis of Salmonella enterica serovar Enteritidis following propionate adaptation
    Article Snippet: Contaminating genomic DNA was removed from the RNA sample using the RNase-Free DNase set (Qiagen). .. The cDNA template was subsequently diluted 10× in preparation for PCR.

    Article Title: Time-resolved mapping of genetic interactions to model rewiring of signaling pathways
    Article Snippet: An optional DNase digestion step was performed using the RNase-Free DNase Set (Qiagen). .. The plate was then centrifuged (2 min, 2000 rpm) and processed for qPCR in a Roche 480 LightCycler using the following PCR program: (i) 10 min at 95°C, (ii) 15 s at 95°C, (iii) 60 s at 60°C, repeat step ii) and iii) 40 times and measure fluorescence at 494 nm-521 nm during step iii).

    Chromatin Immunoprecipitation:

    Article Title: Cancer Immunoediting from Immunosurveillance to Tumor Escape in Microvillus-Formed Niche: A Study of Syngeneic Orthotopic Rat Bladder Cancer Model in Comparison with Human Bladder Cancer 1
    Article Snippet: Cultured AY-27 cells and the entire bladder from each rat were taken and immediately frozen in a bath of liquid nitrogen and stored at -80°C until isolation of RNA using the RNeasy Fibrous Midi Kit and RNase-free DNase Set (Qiagen, Valencia, CA). .. Isolated RNA was analyzed by Affymetrix GeneChip (Affymetrix, Santa Clara, CA) with a RAE 230 A chip that includes more than 15,000 rat genes.

    Real-time Polymerase Chain Reaction:

    Article Title: Smooth muscle cell-specific FoxM1 controls hypoxia-induced pulmonary hypertension
    Article Snippet: We used a miRNeasy Mini Kit (Qiagen, Valencia, CA) and an RNase-Free DNase Set (Qiagen) to extract the total RNA. .. We determined the quantity and quality of total RNA with Nanodrop 2000 spectrophotometer (ThermoScientific, Rockford, IL) and then performed reverse transcription using High Capacity Reverse Transcription kits (Applied Biosystems, Foster City, CA). qRT-PCR was conducted with SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA) on a StepOnePlus or a ViiA 7 Real-Time PCR System (Applied Biosystems).

    Article Title: A neurotoxic regimen of methamphetamine exacerbates the febrile and neuroinflammatory response to a subsequent peripheral immune stimulus
    Article Snippet: Paragraph title: Cytokine mRNA measurement by quantitative real-time PCR ... DNase treatment was performed on a PicoPure column with a Qiagen RNase-free DNase set (Qiagen, Valenica, CA).

    Article Title: Proteomic analysis of Salmonella enterica serovar Enteritidis following propionate adaptation
    Article Snippet: Paragraph title: Quantitative Real Time PCR ... Contaminating genomic DNA was removed from the RNA sample using the RNase-Free DNase set (Qiagen).

    Article Title: Germination and the Early Stages of Seedling Development in Brachypodium distachyon
    Article Snippet: The RNA was purified using Qiagen RNase-Free DNase set (Qiagen, Venlo, Netherlands) and an RNeasy MinElute Cleanup Kit (Qiagen, Venlo, Netherlands). .. The real-time PCR as well as the calculation of the relative expression level were done according to Betekhtin et al. [ ].

    Article Title: Time-resolved mapping of genetic interactions to model rewiring of signaling pathways
    Article Snippet: Paragraph title: qPCR analysis ... An optional DNase digestion step was performed using the RNase-Free DNase Set (Qiagen).

    RNA Extraction:

    Article Title: Pulsed electromagnetic fields increase osteogenetic commitment of MSCs via the mTOR pathway in TNF-α mediated inflammatory conditions: an in-vitro study
    Article Snippet: Paragraph title: RNA extraction and first-strand cDNA synthesis ... RNase-Free DNase Set (Qiagen) from implants were cultured with adipose tissue derived mesenchymal stem cells for 15 and 25 days.

    Article Title: Engineering d‐limonene synthase down‐regulation in orange fruit induces resistance against the fungus Phyllosticta citricarpa through enhanced accumulation of monoterpene alcohols and activation of defence
    Article Snippet: Paragraph title: RNA extraction ... RNA was treated with DNase I (RNase‐Free DNase Set; Qiagen) for real‐time qRT‐PCR analysis and quantified using a NanoDrop spectrophotometer (NanoDrop 2000C; Thermo Scientific, USA).

    Agarose Gel Electrophoresis:

    Article Title: Genome-Wide Association Mapping in a Rice MAGIC Plus Population Detects QTLs and Genes Useful for Biofortification
    Article Snippet: Total RNA was isolated from grains using PureLink Plant RNA Reagent (Life Technologies, CA, United States) and purified using RNeasy Mini Kit with on-column DNAse treatment using RNase-Free DNase Set (Qiagen, CA, United States) as described by the manufacturers. .. RNA was eluted in 30 μl DEPC-treated water, quantified using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, DE, United States), and integrity-checked on agarose gel.

    Preserving:

    Article Title: The effect of cellobiose on the health status of growing rabbits depends on the dietary level of soluble fiber
    Article Snippet: Samples were placed in vials containing 1 mL of RNA preserving solution (RNA Later, Applied Biosystems, Foster City, CA) and frozen at −80°C. .. A DNAse treatment step using RNase-Free DNase Set (Qiagen, Hilden, Germany) was added to prevent genomic DNA contamination.

    Spectrophotometry:

    Article Title: Genome-Wide Association Mapping in a Rice MAGIC Plus Population Detects QTLs and Genes Useful for Biofortification
    Article Snippet: Total RNA was isolated from grains using PureLink Plant RNA Reagent (Life Technologies, CA, United States) and purified using RNeasy Mini Kit with on-column DNAse treatment using RNase-Free DNase Set (Qiagen, CA, United States) as described by the manufacturers. .. RNA was eluted in 30 μl DEPC-treated water, quantified using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, DE, United States), and integrity-checked on agarose gel.

    Article Title: Smooth muscle cell-specific FoxM1 controls hypoxia-induced pulmonary hypertension
    Article Snippet: We used a miRNeasy Mini Kit (Qiagen, Valencia, CA) and an RNase-Free DNase Set (Qiagen) to extract the total RNA. .. We determined the quantity and quality of total RNA with Nanodrop 2000 spectrophotometer (ThermoScientific, Rockford, IL) and then performed reverse transcription using High Capacity Reverse Transcription kits (Applied Biosystems, Foster City, CA). qRT-PCR was conducted with SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA) on a StepOnePlus or a ViiA 7 Real-Time PCR System (Applied Biosystems).

    Article Title: The effect of cellobiose on the health status of growing rabbits depends on the dietary level of soluble fiber
    Article Snippet: A DNAse treatment step using RNase-Free DNase Set (Qiagen, Hilden, Germany) was added to prevent genomic DNA contamination. .. The RNA concentration was measured by spectrophotometry (Epoch, BioTek, Winooski, VT) combined with the Take3 Micro-Volume Plate (BioTek, Santa Barbara, CA).

    Article Title: Germination and the Early Stages of Seedling Development in Brachypodium distachyon
    Article Snippet: The RNA was purified using Qiagen RNase-Free DNase set (Qiagen, Venlo, Netherlands) and an RNeasy MinElute Cleanup Kit (Qiagen, Venlo, Netherlands). .. The concentration and quality of the isolated RNA was evaluated using an ND-1000 NanoDrop spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    Article Title: Pulsed electromagnetic fields increase osteogenetic commitment of MSCs via the mTOR pathway in TNF-α mediated inflammatory conditions: an in-vitro study
    Article Snippet: RNase-Free DNase Set (Qiagen) from implants were cultured with adipose tissue derived mesenchymal stem cells for 15 and 25 days. .. The RNA quality and concentration of the samples were measured using a NanoDropTM ND-1000 Spectrophotometer (Thermo Scientific).

    Article Title: Engineering d‐limonene synthase down‐regulation in orange fruit induces resistance against the fungus Phyllosticta citricarpa through enhanced accumulation of monoterpene alcohols and activation of defence
    Article Snippet: .. RNA was treated with DNase I (RNase‐Free DNase Set; Qiagen) for real‐time qRT‐PCR analysis and quantified using a NanoDrop spectrophotometer (NanoDrop 2000C; Thermo Scientific, USA). .. Microarray assays were performed with the flavedo of two independent AS (AS1 and AS7) and EV transgenic ‘Navelina’ mature orange fruit, inoculated with P. citricarpa , comparing randomly selected AS vs. EV samples on the same slide with three biological replicates for each genotype.

    Produced:

    Article Title: Germination and the Early Stages of Seedling Development in Brachypodium distachyon
    Article Snippet: The RNA was purified using Qiagen RNase-Free DNase set (Qiagen, Venlo, Netherlands) and an RNeasy MinElute Cleanup Kit (Qiagen, Venlo, Netherlands). .. First-strand cDNA was produced using a Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, Waltham, MA, USA).

    Concentration Assay:

    Article Title: The effect of cellobiose on the health status of growing rabbits depends on the dietary level of soluble fiber
    Article Snippet: A DNAse treatment step using RNase-Free DNase Set (Qiagen, Hilden, Germany) was added to prevent genomic DNA contamination. .. The RNA concentration was measured by spectrophotometry (Epoch, BioTek, Winooski, VT) combined with the Take3 Micro-Volume Plate (BioTek, Santa Barbara, CA).

    Article Title: Germination and the Early Stages of Seedling Development in Brachypodium distachyon
    Article Snippet: The RNA was purified using Qiagen RNase-Free DNase set (Qiagen, Venlo, Netherlands) and an RNeasy MinElute Cleanup Kit (Qiagen, Venlo, Netherlands). .. The concentration and quality of the isolated RNA was evaluated using an ND-1000 NanoDrop spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    Article Title: Pulsed electromagnetic fields increase osteogenetic commitment of MSCs via the mTOR pathway in TNF-α mediated inflammatory conditions: an in-vitro study
    Article Snippet: RNase-Free DNase Set (Qiagen) from implants were cultured with adipose tissue derived mesenchymal stem cells for 15 and 25 days. .. The RNA quality and concentration of the samples were measured using a NanoDropTM ND-1000 Spectrophotometer (Thermo Scientific).

    Article Title: Circ0043898 acts as a tumor inhibitor and performs regulatory effect on the inhibition of esophageal carcinoma
    Article Snippet: RNA Clean XP Kit (Beckman Coulter, CA, UA) and RNase-free DNase Set (QIAGEN, Germany) were used for RNA cleanup. .. The quality and concentration of the RNA were determined by NanoDrop 2000 (Thermo Fisher, USA).

    Lysis:

    Article Title: Proteomic analysis of Salmonella enterica serovar Enteritidis following propionate adaptation
    Article Snippet: Enzymatic lysis of cell wall material was performed by incubating freshly harvested cells in TE buffer containing 1 mg/mL lysozyme for five minutes at room temperature. .. Contaminating genomic DNA was removed from the RNA sample using the RNase-Free DNase set (Qiagen).

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  • 90
    Qiagen on column dnase digestion step the rnase free dnase set qiagen
    On Column Dnase Digestion Step The Rnase Free Dnase Set Qiagen, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/on column dnase digestion step the rnase free dnase set qiagen/product/Qiagen
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    on column dnase digestion step the rnase free dnase set qiagen - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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