oligotex mrna mini kit  (Qiagen)

 
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    Name:
    Oligotex mRNA Mini Kit
    Description:
    For mRNA purification from total RNA and for in vitro transcript cleanup Kit contents Qiagen Oligotex mRNA Mini Kit 12 preps 250g Sample 20 to 100L Elution Volume Total RNA Sample Poly A mRNA Purification Polystyrene latex Particles Technology Spin Column Format Manual Processing 90 Recovery Yield Ideal for PCR Real time PCR cDNA Synthesis In vitro Translation Microarray Transfection Includes 200L Oligotex Suspension Small Spin Columns 1 5mL Collection Tubes RNase free Reagents and Buffers Benefits High recovery of pure mRNA in as little as 30 minutes No oligo dT cellulose or ethanol precipitation Flexibility for use with widely varying amounts of star
    Catalog Number:
    70022
    Price:
    341
    Category:
    Oligotex mRNA Mini Kit
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    Structured Review

    Qiagen oligotex mrna mini kit
    Oligotex mRNA Mini Kit
    For mRNA purification from total RNA and for in vitro transcript cleanup Kit contents Qiagen Oligotex mRNA Mini Kit 12 preps 250g Sample 20 to 100L Elution Volume Total RNA Sample Poly A mRNA Purification Polystyrene latex Particles Technology Spin Column Format Manual Processing 90 Recovery Yield Ideal for PCR Real time PCR cDNA Synthesis In vitro Translation Microarray Transfection Includes 200L Oligotex Suspension Small Spin Columns 1 5mL Collection Tubes RNase free Reagents and Buffers Benefits High recovery of pure mRNA in as little as 30 minutes No oligo dT cellulose or ethanol precipitation Flexibility for use with widely varying amounts of star
    https://www.bioz.com/result/oligotex mrna mini kit/product/Qiagen
    Average 99 stars, based on 225 article reviews
    Price from $9.99 to $1999.99
    oligotex mrna mini kit - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Comparative Transcriptional Profiling and Physiological Responses of Two Contrasting Oat Genotypes under Salt Stress
    Article Snippet: .. RNA extraction and double-stranded cDNA synthesis Total RNA was extracted from whole plants using the RNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s instructions. mRNA was isolated from the qualified total RNA using the Oligotex mRNA Mini Kit (Qiagen). mRNA extracted from the salt-stressed oat seedlings after different durations was prepared for cDNA synthesis. .. The quality of the total RNA, mRNA, and final double-stranded cDNA samples was determined with an Agilent 2100 Bioanalyzer (Agilent Technologies).

    Article Title: Expression of chemokine/cytokine genes and immune cell recruitment following the instillation of Mycobacterium bovis, bacillus Calmette-Gu?rin or Lactobacillus rhamnosus strain GG in the healthy murine bladder
    Article Snippet: .. Briefly, total RNA was extracted from each bladder using Trizol and poly (A)+ RNA was isolated using the Oligotex messenger RNA (mRNA) kit (Qiagen, Hilden, Germany) and was used to synthesize biotinylated complementary RNA (TrueLabeling-AMP Linear RNA Amplification Kit; SuperArray Bioscience Corporation), which was hybridized to the membrane for 24 hr. ..

    Article Title: Recombinant Major Vault Protein Is Targeted to Neuritic Tips of PC12 Cells
    Article Snippet: .. Northern Blot Analysis For analyzing length and yield of MVP transcripts, mRNA from CHO or PC12 cells was isolated using an mRNA kit (Oligotex Direct; Quiagen). .. To further investigate the influence of the differentiation state on the expression of the MVP transcript, PC12 cells were cultivated for 3 d in the presence of β-NGF (5 ng/ml; Sigma Chemical Co. ).

    Article Title: Comparative Transcriptional Profiling and Physiological Responses of Two Contrasting Oat Genotypes under Salt Stress
    Article Snippet: .. Total RNA was extracted from whole plants using the RNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s instructions. mRNA was isolated from the qualified total RNA using the Oligotex mRNA Mini Kit (Qiagen). mRNA extracted from the salt-stressed oat seedlings after different durations was prepared for cDNA synthesis. .. The quality of the total RNA, mRNA, and final double-stranded cDNA samples was determined with an Agilent 2100 Bioanalyzer (Agilent Technologies).

    RNA Extraction:

    Article Title: Comparative Transcriptional Profiling and Physiological Responses of Two Contrasting Oat Genotypes under Salt Stress
    Article Snippet: .. RNA extraction and double-stranded cDNA synthesis Total RNA was extracted from whole plants using the RNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s instructions. mRNA was isolated from the qualified total RNA using the Oligotex mRNA Mini Kit (Qiagen). mRNA extracted from the salt-stressed oat seedlings after different durations was prepared for cDNA synthesis. .. The quality of the total RNA, mRNA, and final double-stranded cDNA samples was determined with an Agilent 2100 Bioanalyzer (Agilent Technologies).

    Amplification:

    Article Title: Expression of chemokine/cytokine genes and immune cell recruitment following the instillation of Mycobacterium bovis, bacillus Calmette-Gu?rin or Lactobacillus rhamnosus strain GG in the healthy murine bladder
    Article Snippet: .. Briefly, total RNA was extracted from each bladder using Trizol and poly (A)+ RNA was isolated using the Oligotex messenger RNA (mRNA) kit (Qiagen, Hilden, Germany) and was used to synthesize biotinylated complementary RNA (TrueLabeling-AMP Linear RNA Amplification Kit; SuperArray Bioscience Corporation), which was hybridized to the membrane for 24 hr. ..

    Northern Blot:

    Article Title: Recombinant Major Vault Protein Is Targeted to Neuritic Tips of PC12 Cells
    Article Snippet: .. Northern Blot Analysis For analyzing length and yield of MVP transcripts, mRNA from CHO or PC12 cells was isolated using an mRNA kit (Oligotex Direct; Quiagen). .. To further investigate the influence of the differentiation state on the expression of the MVP transcript, PC12 cells were cultivated for 3 d in the presence of β-NGF (5 ng/ml; Sigma Chemical Co. ).

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  • mrna  (Qiagen)
    99
    Qiagen mrna
    Validation of the expression patterns of selected oat genes by qRT-PCR. ( A ) Changes in the relative <t>mRNA</t> levels of 18 selected genes as determined by <t>RNA-Seq.</t> The Y axis indicates the relative fold change in transcript abundance under conditions of salt stress in relation to the control. S: ‘Huazao-2’ under normal growth conditions (1) and after 2 (2), 4 (3), 8 (4), 12 (5), and 24 (6) h of salt stress treatment. T: ‘Hanyou-5’ under normal growth conditions (1) and after 2 (2), 4 (3), 8 (4), 12 (5), and 24 (6) h of salt stress treatment. ( B ) Changes in the relative mRNA levels of 18 selected genes as determined by qRT-PCR. The qRT-PCR experiment was carried out with three biological replicates, and the actin gene was used as an internal control. The results are presented as target/reference ratios normalized by the internal control.
    Mrna, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mrna/product/Qiagen
    Average 99 stars, based on 1619 article reviews
    Price from $9.99 to $1999.99
    mrna - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Qiagen m rna isolation kits
    <t>mRNA</t> expression of neurotrophin receptors in eosinophils. Expression of mRNA for β-actin (positive control, 194 bp), p75NTR (147 bp), trkA (479 bp), trkB (221 bp), and trkC (356 bp) in peripheral blood eosinophils (PB-Eos) or BALF eosinophils, respectively, from asthmatics 18 h after SAP. M, 100 bp DNA ladder; <t>RNA,</t> RNA control.
    M Rna Isolation Kits, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m rna isolation kits/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m rna isolation kits - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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    Validation of the expression patterns of selected oat genes by qRT-PCR. ( A ) Changes in the relative mRNA levels of 18 selected genes as determined by RNA-Seq. The Y axis indicates the relative fold change in transcript abundance under conditions of salt stress in relation to the control. S: ‘Huazao-2’ under normal growth conditions (1) and after 2 (2), 4 (3), 8 (4), 12 (5), and 24 (6) h of salt stress treatment. T: ‘Hanyou-5’ under normal growth conditions (1) and after 2 (2), 4 (3), 8 (4), 12 (5), and 24 (6) h of salt stress treatment. ( B ) Changes in the relative mRNA levels of 18 selected genes as determined by qRT-PCR. The qRT-PCR experiment was carried out with three biological replicates, and the actin gene was used as an internal control. The results are presented as target/reference ratios normalized by the internal control.

    Journal: Scientific Reports

    Article Title: Comparative Transcriptional Profiling and Physiological Responses of Two Contrasting Oat Genotypes under Salt Stress

    doi: 10.1038/s41598-018-34505-5

    Figure Lengend Snippet: Validation of the expression patterns of selected oat genes by qRT-PCR. ( A ) Changes in the relative mRNA levels of 18 selected genes as determined by RNA-Seq. The Y axis indicates the relative fold change in transcript abundance under conditions of salt stress in relation to the control. S: ‘Huazao-2’ under normal growth conditions (1) and after 2 (2), 4 (3), 8 (4), 12 (5), and 24 (6) h of salt stress treatment. T: ‘Hanyou-5’ under normal growth conditions (1) and after 2 (2), 4 (3), 8 (4), 12 (5), and 24 (6) h of salt stress treatment. ( B ) Changes in the relative mRNA levels of 18 selected genes as determined by qRT-PCR. The qRT-PCR experiment was carried out with three biological replicates, and the actin gene was used as an internal control. The results are presented as target/reference ratios normalized by the internal control.

    Article Snippet: RNA extraction and double-stranded cDNA synthesis Total RNA was extracted from whole plants using the RNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s instructions. mRNA was isolated from the qualified total RNA using the Oligotex mRNA Mini Kit (Qiagen). mRNA extracted from the salt-stressed oat seedlings after different durations was prepared for cDNA synthesis.

    Techniques: Expressing, Quantitative RT-PCR, RNA Sequencing Assay

    hCycT1 and CDK9 duplex siRNAs inhibit HIV-1 Tat transactivation in Magi cells. (A) Analysis of hCycT1 and CDK9 RNAi activities in Magi cells by Western blotting. Magi cells were cotransfected with pTat-RFP plasmid and various siRNAs. Cells were harvested at 48 h posttransfection. Proteins were resolved by SDS-10% PAGE, transferred onto PVDF membranes, and immunoblotted with antibodies against hCycT1 (upper panel) and CDK9 (lower panel). Lanes 1 and 2, RNAi activities in Magi cells treated with antisense (as) strands of hCycT1 and CDK9 siRNAs; lanes 3 and 4, RNAi activities of cells treated with double-stranded siRNAs targeting hCycT1 and CDK9; lanes 5 and 6, RNAi activities in cells treated with mutant hCycT1 siRNA (hCycT1 mm) or mutant CDK9 siRNA (CDK9 mm). A double-stranded GFP siRNA was used as an unrelated control (lane 7), while double-stranded Tat siRNA was used to target mRNA encoding Tat (lane 8). (B) Photomicrographs of β-Gal-stained Magi cells. Magi cells were untransfected (panels a, c, e, and g) or transfected (panels b, d, f, and h) with pTat-RFP in the presence of mismatched hCycT1 siRNA (mm) (panels b and f) or hCycT1 double-stranded siRNA (panels d and h). LTR activation (represented by β-Gal-stained cells) was reduced in the hCycT1 double-stranded siRNA-treated cells (panels d and h). (C) Effect of P-TEFb silencing by RNAi on Tat transactivation in Magi cells. Twenty-four hours after pretreatment of Magi cells with siRNA, the cells were cotransfected with pTat-RFP plasmid and various siRNAs. Cells were harvested at 48 h post-pTat-RFP transfection, and the activity of β-Gal in clear cell lysates was measured (see Materials and Methods). Tat transactivation was determined by the ratio of β-Gal activity in pTat-RFP-transfected cells to that in cells without pTat-RFP treatment. Inhibitory activity was determined by normalizing the Tat transactivation activity to the amount of Tat-RFP protein (see Materials and Methods) in the presence or absence of siRNA treatment. Bar 1, Tat-RFP transfection (mock). Magi cells were cotransfected with double-stranded siRNAs targeting hCycT1 and CDK9 (bars 4 and 5), with antisense (as) RNA strands (bars 2 and 3), or with mutant (mm) siRNAs (bars 6 and 7). Double-stranded GFP siRNA was used as an unrelated control (bar 8), while a double-stranded Tat siRNA targeting the mRNA encoding the Tat sequence was used as a positive control (bar 9). Means ± standard deviations (SD) of two experiments are shown.

    Journal: Journal of Virology

    Article Title: Inhibition of Human Immunodeficiency Virus Type 1 Replication by RNA Interference Directed against Human Transcription Elongation Factor P-TEFb (CDK9/CyclinT1)

    doi: 10.1128/JVI.78.5.2517-2529.2004

    Figure Lengend Snippet: hCycT1 and CDK9 duplex siRNAs inhibit HIV-1 Tat transactivation in Magi cells. (A) Analysis of hCycT1 and CDK9 RNAi activities in Magi cells by Western blotting. Magi cells were cotransfected with pTat-RFP plasmid and various siRNAs. Cells were harvested at 48 h posttransfection. Proteins were resolved by SDS-10% PAGE, transferred onto PVDF membranes, and immunoblotted with antibodies against hCycT1 (upper panel) and CDK9 (lower panel). Lanes 1 and 2, RNAi activities in Magi cells treated with antisense (as) strands of hCycT1 and CDK9 siRNAs; lanes 3 and 4, RNAi activities of cells treated with double-stranded siRNAs targeting hCycT1 and CDK9; lanes 5 and 6, RNAi activities in cells treated with mutant hCycT1 siRNA (hCycT1 mm) or mutant CDK9 siRNA (CDK9 mm). A double-stranded GFP siRNA was used as an unrelated control (lane 7), while double-stranded Tat siRNA was used to target mRNA encoding Tat (lane 8). (B) Photomicrographs of β-Gal-stained Magi cells. Magi cells were untransfected (panels a, c, e, and g) or transfected (panels b, d, f, and h) with pTat-RFP in the presence of mismatched hCycT1 siRNA (mm) (panels b and f) or hCycT1 double-stranded siRNA (panels d and h). LTR activation (represented by β-Gal-stained cells) was reduced in the hCycT1 double-stranded siRNA-treated cells (panels d and h). (C) Effect of P-TEFb silencing by RNAi on Tat transactivation in Magi cells. Twenty-four hours after pretreatment of Magi cells with siRNA, the cells were cotransfected with pTat-RFP plasmid and various siRNAs. Cells were harvested at 48 h post-pTat-RFP transfection, and the activity of β-Gal in clear cell lysates was measured (see Materials and Methods). Tat transactivation was determined by the ratio of β-Gal activity in pTat-RFP-transfected cells to that in cells without pTat-RFP treatment. Inhibitory activity was determined by normalizing the Tat transactivation activity to the amount of Tat-RFP protein (see Materials and Methods) in the presence or absence of siRNA treatment. Bar 1, Tat-RFP transfection (mock). Magi cells were cotransfected with double-stranded siRNAs targeting hCycT1 and CDK9 (bars 4 and 5), with antisense (as) RNA strands (bars 2 and 3), or with mutant (mm) siRNAs (bars 6 and 7). Double-stranded GFP siRNA was used as an unrelated control (bar 8), while a double-stranded Tat siRNA targeting the mRNA encoding the Tat sequence was used as a positive control (bar 9). Means ± standard deviations (SD) of two experiments are shown.

    Article Snippet: The total cellular mRNA was prepared from HeLa cells, with or without hCycT1 or CDK9 siRNA treatment, by using a Qiagen RNA mini kit followed by an Oligotex mRNA mini kit (Qiagen).

    Techniques: Western Blot, Plasmid Preparation, Polyacrylamide Gel Electrophoresis, Mutagenesis, Staining, Transfection, Activation Assay, Activity Assay, Sequencing, Positive Control

    Expression of MVP in CHO and PC12 cells. (A) Western blot of total protein extracts of CHO (lane 1) and PC12 (lane 2) cells using polyclonal affinity-purified anti–rat vault antibody. 50 μg of protein was loaded per lane. (B) Northern blot of mRNA isolated from CHO (lane 1) and PC12 (lane 2) cells using a DIG-labeled RNA encoding rat MVP as a probe. 800 ng of mRNA was applied per lane. The hybridization signal for β-actin (lower graph) served as a control. Sizes of RNA markers are indicated (left). Immunocytochemistry of CHO (C) and PC12 (D) cells using the same antibody as in B demonstrated the overall distribution of vault particles. In D, localization of vaults in neuritic extensions and tips is indicated (arrows). Bar, 20 μm.

    Journal: The Journal of Cell Biology

    Article Title: Recombinant Major Vault Protein Is Targeted to Neuritic Tips of PC12 Cells

    doi:

    Figure Lengend Snippet: Expression of MVP in CHO and PC12 cells. (A) Western blot of total protein extracts of CHO (lane 1) and PC12 (lane 2) cells using polyclonal affinity-purified anti–rat vault antibody. 50 μg of protein was loaded per lane. (B) Northern blot of mRNA isolated from CHO (lane 1) and PC12 (lane 2) cells using a DIG-labeled RNA encoding rat MVP as a probe. 800 ng of mRNA was applied per lane. The hybridization signal for β-actin (lower graph) served as a control. Sizes of RNA markers are indicated (left). Immunocytochemistry of CHO (C) and PC12 (D) cells using the same antibody as in B demonstrated the overall distribution of vault particles. In D, localization of vaults in neuritic extensions and tips is indicated (arrows). Bar, 20 μm.

    Article Snippet: Northern Blot Analysis For analyzing length and yield of MVP transcripts, mRNA from CHO or PC12 cells was isolated using an mRNA kit (Oligotex Direct; Quiagen).

    Techniques: Expressing, Western Blot, Affinity Purification, Northern Blot, Isolation, Labeling, Hybridization, Immunocytochemistry

    Transient expression of rat vMVP in CHO cells. (A) Schematic drawing of the engineered construct denoted pvMVP for heterologous expression of rat MVP in mammalian cell lines is shown. Note that expression vector and cDNA sequence encoding MVP are not in scale. (B) Northern blot using a DIG-labeled RNA encoding rat MVP as a probe is shown. 800 ng of mRNA was applied per lane. Lane 1 shows mRNA isolated from nontransfected CHO cells; lane 2 shows mRNA from CHO cells transfected with pvMVP; and lane 3 shows mRNA from transfected CHO cells treated with sodium butyrate. The hybridization signal for β-actin (lower graph) served as a control. Arrows indicate the position of the endogenous MVP transcript (left and right), overexpressed vMVP, and β-actin (both right). The position of marker RNA is given in kilobases. (C) Western blot using total protein extract of CHO cells, polyclonal affinity-purified anti–rat vault antibody (upper graph), or anti-VSVG mAb (lower graph) is shown. (Lane 1) Nontransfected; (lane 2) pvMVP-transfected, and (lane 3) pvMVP-transfected and butyrate-treated cells. 30 μg of protein was applied per lane. Protein bands detected by anti-MVP antibody (MVP*) or anti-VSVG antibody (vMVP) are marked by arrows (right). Note, the anti-MVP antibody detects endogenous and recombinant MVP. The size of marker polypeptides is given in kilodaltons (left).

    Journal: The Journal of Cell Biology

    Article Title: Recombinant Major Vault Protein Is Targeted to Neuritic Tips of PC12 Cells

    doi:

    Figure Lengend Snippet: Transient expression of rat vMVP in CHO cells. (A) Schematic drawing of the engineered construct denoted pvMVP for heterologous expression of rat MVP in mammalian cell lines is shown. Note that expression vector and cDNA sequence encoding MVP are not in scale. (B) Northern blot using a DIG-labeled RNA encoding rat MVP as a probe is shown. 800 ng of mRNA was applied per lane. Lane 1 shows mRNA isolated from nontransfected CHO cells; lane 2 shows mRNA from CHO cells transfected with pvMVP; and lane 3 shows mRNA from transfected CHO cells treated with sodium butyrate. The hybridization signal for β-actin (lower graph) served as a control. Arrows indicate the position of the endogenous MVP transcript (left and right), overexpressed vMVP, and β-actin (both right). The position of marker RNA is given in kilobases. (C) Western blot using total protein extract of CHO cells, polyclonal affinity-purified anti–rat vault antibody (upper graph), or anti-VSVG mAb (lower graph) is shown. (Lane 1) Nontransfected; (lane 2) pvMVP-transfected, and (lane 3) pvMVP-transfected and butyrate-treated cells. 30 μg of protein was applied per lane. Protein bands detected by anti-MVP antibody (MVP*) or anti-VSVG antibody (vMVP) are marked by arrows (right). Note, the anti-MVP antibody detects endogenous and recombinant MVP. The size of marker polypeptides is given in kilodaltons (left).

    Article Snippet: Northern Blot Analysis For analyzing length and yield of MVP transcripts, mRNA from CHO or PC12 cells was isolated using an mRNA kit (Oligotex Direct; Quiagen).

    Techniques: Expressing, Construct, Plasmid Preparation, Sequencing, Northern Blot, Labeling, Isolation, Transfection, Hybridization, Marker, Western Blot, Affinity Purification, Recombinant

    mRNA expression of neurotrophin receptors in eosinophils. Expression of mRNA for β-actin (positive control, 194 bp), p75NTR (147 bp), trkA (479 bp), trkB (221 bp), and trkC (356 bp) in peripheral blood eosinophils (PB-Eos) or BALF eosinophils, respectively, from asthmatics 18 h after SAP. M, 100 bp DNA ladder; RNA, RNA control.

    Journal: The Journal of Experimental Medicine

    Article Title: The Neurotrophins Nerve Growth Factor, Brain-derived Neurotrophic Factor, Neurotrophin-3, and Neurotrophin-4 Are Survival and Activation Factors for Eosinophils in Patients with Allergic Bronchial Asthma

    doi: 10.1084/jem.20010897

    Figure Lengend Snippet: mRNA expression of neurotrophin receptors in eosinophils. Expression of mRNA for β-actin (positive control, 194 bp), p75NTR (147 bp), trkA (479 bp), trkB (221 bp), and trkC (356 bp) in peripheral blood eosinophils (PB-Eos) or BALF eosinophils, respectively, from asthmatics 18 h after SAP. M, 100 bp DNA ladder; RNA, RNA control.

    Article Snippet: Neurotrophin receptor mRNA expression of purified eosinophils obtained 18 h after SAP from peripheral blood or BALF fluid of patients with asthma, respectively, was examined. mRNA was extracted from total cellular RNA using commercially available (m)RNA-isolation kits (RNeasy Mini Kit and Oligotex mRNA Isolation Kit, both QIAGEN).

    Techniques: Expressing, Positive Control