oligotex mrna mini kit  (Qiagen)


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  • 99
    Name:
    Oligotex mRNA Mini Kit
    Description:
    For mRNA purification from total RNA and for in vitro transcript cleanup Kit contents Qiagen Oligotex mRNA Mini Kit 12 preps 250g Sample 20 to 100L Elution Volume Total RNA Sample Poly A mRNA Purification Polystyrene latex Particles Technology Spin Column Format Manual Processing 90 Recovery Yield Ideal for PCR Real time PCR cDNA Synthesis In vitro Translation Microarray Transfection Includes 200L Oligotex Suspension Small Spin Columns 1 5mL Collection Tubes RNase free Reagents and Buffers Benefits High recovery of pure mRNA in as little as 30 minutes No oligo dT cellulose or ethanol precipitation Flexibility for use with widely varying amounts of star
    Catalog Number:
    70022
    Price:
    341
    Category:
    Oligotex mRNA Mini Kit
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    Structured Review

    Qiagen oligotex mrna mini kit
    Oligotex mRNA Mini Kit
    For mRNA purification from total RNA and for in vitro transcript cleanup Kit contents Qiagen Oligotex mRNA Mini Kit 12 preps 250g Sample 20 to 100L Elution Volume Total RNA Sample Poly A mRNA Purification Polystyrene latex Particles Technology Spin Column Format Manual Processing 90 Recovery Yield Ideal for PCR Real time PCR cDNA Synthesis In vitro Translation Microarray Transfection Includes 200L Oligotex Suspension Small Spin Columns 1 5mL Collection Tubes RNase free Reagents and Buffers Benefits High recovery of pure mRNA in as little as 30 minutes No oligo dT cellulose or ethanol precipitation Flexibility for use with widely varying amounts of star
    https://www.bioz.com/result/oligotex mrna mini kit/product/Qiagen
    Average 99 stars, based on 212 article reviews
    Price from $9.99 to $1999.99
    oligotex mrna mini kit - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    RNA Extraction:

    Article Title: Destabilization of the human RED–SMU1 splicing complex as a basis for host-directed antiinfluenza strategy
    Article Snippet: Paragraph title: RNA Extraction and Reverse Transcription-Quantitative PCR. ... Total RNA and poly(A)+ RNAs were extracted successively using the RNeasy Mini Kit (Qiagen) and Oligotex mRNA Mini Kit (Qiagen) following the manufacturer’s protocol.

    Real-time Polymerase Chain Reaction:

    Article Title: Destabilization of the human RED–SMU1 splicing complex as a basis for host-directed antiinfluenza strategy
    Article Snippet: Total RNA and poly(A)+ RNAs were extracted successively using the RNeasy Mini Kit (Qiagen) and Oligotex mRNA Mini Kit (Qiagen) following the manufacturer’s protocol. .. Quantitative real-time PCR was performed on 2 μL of a 1/10 dilution of the RT reaction using Luminaris Color Probe qPCR Master Mix (Thermo Fisher Scientific) and a Light Cycler 480 (Roche).

    Sequencing:

    Article Title: CD19-antigen specific nanoscale liposomal formulation of a SYK P-site inhibitor causes apoptotic destruction of human B-precursor leukemia cells
    Article Snippet: .. To determine the unique cDNA sequence of the complementarity determining regions (CDR) of this MoAb, mRNA was extracted from around 107 ARESX1 hybridoma cells using Qiagen Oligotex mRNA Mini kit (Cat. No70022). .. The first-strand cDNA was generated with oligo (dT) primers using Superscript III reverse transcriptase (Invitrogen, San Diego, CA).

    Generated:

    Article Title: CD19-antigen specific nanoscale liposomal formulation of a SYK P-site inhibitor causes apoptotic destruction of human B-precursor leukemia cells
    Article Snippet: To determine the unique cDNA sequence of the complementarity determining regions (CDR) of this MoAb, mRNA was extracted from around 107 ARESX1 hybridoma cells using Qiagen Oligotex mRNA Mini kit (Cat. No70022). .. The first-strand cDNA was generated with oligo (dT) primers using Superscript III reverse transcriptase (Invitrogen, San Diego, CA).

    DNA Sequencing:

    Article Title: CD19-antigen specific nanoscale liposomal formulation of a SYK P-site inhibitor causes apoptotic destruction of human B-precursor leukemia cells
    Article Snippet: To determine the unique cDNA sequence of the complementarity determining regions (CDR) of this MoAb, mRNA was extracted from around 107 ARESX1 hybridoma cells using Qiagen Oligotex mRNA Mini kit (Cat. No70022). .. The VH (~390-bp) and VL (~320-bp) PCR products were subsequently ligated to the pCR4Blunt-TOPO vector (Cat. No K2835-20, Invitrogen) for DNA sequencing using universal forward (GTAAAACGACGGCCAG) and reverse (CAGGAAACAGCTATGAC) primers (Cat. No N520-02 and N530-02).

    Polymerase Chain Reaction:

    Article Title: CD19-antigen specific nanoscale liposomal formulation of a SYK P-site inhibitor causes apoptotic destruction of human B-precursor leukemia cells
    Article Snippet: To determine the unique cDNA sequence of the complementarity determining regions (CDR) of this MoAb, mRNA was extracted from around 107 ARESX1 hybridoma cells using Qiagen Oligotex mRNA Mini kit (Cat. No70022). .. The cDNA fragments encoding the heavy chain and the light chain variable regions were then obtained by PCR using the high fidelity Phusion kit (E0553s, New England Biolabs) with the Heavy Primers Mix (Cat NO 27-1586-01, GE Healthcare) and the Light Primers Mix (Cat No 27-1583-01, GE Healthcare), respectively.

    Article Title: Destabilization of the human RED–SMU1 splicing complex as a basis for host-directed antiinfluenza strategy
    Article Snippet: Paragraph title: RNA Extraction and Reverse Transcription-Quantitative PCR. ... Total RNA and poly(A)+ RNAs were extracted successively using the RNeasy Mini Kit (Qiagen) and Oligotex mRNA Mini Kit (Qiagen) following the manufacturer’s protocol.

    Plasmid Preparation:

    Article Title: CD19-antigen specific nanoscale liposomal formulation of a SYK P-site inhibitor causes apoptotic destruction of human B-precursor leukemia cells
    Article Snippet: To determine the unique cDNA sequence of the complementarity determining regions (CDR) of this MoAb, mRNA was extracted from around 107 ARESX1 hybridoma cells using Qiagen Oligotex mRNA Mini kit (Cat. No70022). .. The VH (~390-bp) and VL (~320-bp) PCR products were subsequently ligated to the pCR4Blunt-TOPO vector (Cat. No K2835-20, Invitrogen) for DNA sequencing using universal forward (GTAAAACGACGGCCAG) and reverse (CAGGAAACAGCTATGAC) primers (Cat. No N520-02 and N530-02).

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  • 90
    Qiagen oligotex mrna mini kit
    Oligotex Mrna Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligotex mrna mini kit/product/Qiagen
    Average 90 stars, based on 212 article reviews
    Price from $9.99 to $1999.99
    oligotex mrna mini kit - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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