Structured Review

Thermo Fisher oligos
Oligos, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oligos/product/Thermo Fisher
Average 93 stars, based on 129 article reviews
Price from $9.99 to $1999.99
oligos - by Bioz Stars, 2020-05
93/100 stars

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Small Interfering RNA:

Article Title: Kaposi's Sarcoma-Associated Herpesvirus Downregulates Transforming Growth Factor β2 To Promote Enhanced Stability of Capillary-Like Tube Formation
Article Snippet: .. Small interfering RNA (siRNA) specific to gp130 and negative-control oligonucleotides were designed and synthesized by Ambion (Austin, TX). .. The following oligonucleotide sequences were used: sense oligonucleotide 5′-GGC AUA CCU UAA ACA AGC UdTdT-3′ for gp130 (Ambion identification no. 106709) and sense oligonucleotide 5′-AGU ACU GCU UAC GAU ACG GdTdT-3′ for negative-control siRNA.

Transfection:

Article Title: MicroRNA 452 Regulates Cell Proliferation, Cell Migration, and Angiogenesis in Colorectal Cancer by Suppressing VEGFA Expression
Article Snippet: .. The MIR452 mimic (hsa-miR-452, pre-miR miRNA precursor AM17100, product ID: PM12946) and negative control oligonucleotides were commercially synthesized (Ambion, Austin, TX, USA), and used at 50 nmol/mL for transfections. .. The transfections were performed with Lipofectamine RNAiMAX (Invitrogen) or siPORT NeoFX transfection agent (Ambion) according to the manufacturers’ recommendations.

Article Title: Latent KSHV Infection of Endothelial Cells Induces Integrin Beta3 to Activate Angiogenic Phenotypes
Article Snippet: .. Transfection of siRNA siRNA specific to integrin β3 and negative-control oligonucleotides were designed and synthesized by Ambion (Austin, TX). .. The following oligonucleotide sequences were used: integrin β3 (Ambion identification [ID] no. 112581; sense, 5′-GCU AAU UCU UUG ACC UGU UdTdT-3′ ) and negative-control siRNA (sense, 5′-AGU ACU GCU UAC GAU ACG GdTdT-3′ ).

Negative Control:

Article Title: Ets-1 Is Required for the Activation of VEGFR3 during Latent Kaposi's Sarcoma-Associated Herpesvirus Infection of Endothelial Cells
Article Snippet: .. siRNAs specific to gp130, Ets-1, and negative-control oligonucleotides were designed and synthesized by Ambion (Austin, TX). .. The following oligonucleotide sequences were used: gp130 (Ambion identification [ID] no. 106709; sense, 5′-GGC AUA CCU UAA ACA AGC UdTdT-3′), Ets-1 (ID no. 146635; sense, 5′-GCA UAG AGA GCU ACG AUA GdTdT-3′), and negative-control siRNA (sense, 5′-AGU ACU GCU UAC GAU ACG GdTdT-3′).

Article Title: MicroRNA 452 Regulates Cell Proliferation, Cell Migration, and Angiogenesis in Colorectal Cancer by Suppressing VEGFA Expression
Article Snippet: .. The MIR452 mimic (hsa-miR-452, pre-miR miRNA precursor AM17100, product ID: PM12946) and negative control oligonucleotides were commercially synthesized (Ambion, Austin, TX, USA), and used at 50 nmol/mL for transfections. .. The transfections were performed with Lipofectamine RNAiMAX (Invitrogen) or siPORT NeoFX transfection agent (Ambion) according to the manufacturers’ recommendations.

Article Title: Activation of Akt through gp130 Receptor Signaling Is Required for Kaposi's Sarcoma-Associated Herpesvirus-Induced Lymphatic Reprogramming of Endothelial Cells ▿
Article Snippet: .. siRNA specific to gp130, Akt1, STAT3, Prox1, and negative-control oligonucleotides were designed and synthesized by Ambion (Austin, TX). .. The following oligonucleotide sequences were used: gp130 (Ambion identification [ID] no. 106709; sense, 5′-GGC AUA CCU UAA ACA AGC UdTdT-3′), Akt1 (ID no. 633; sense, 5′-GGG CAC UUU CGG CAA GGU GdTdT-3′), STAT3 (ID no. 116558; sense 5′-GCA CAA UCU ACG AAG AAU CdTdT-3′), Prox1 (ID no. 106879; sense, 5′-GGG AAU UUG UUA ACG AUG CdTdT-3′), VEGFR-1A (ID no. 190; 5′-GGU UCA AAA UUA AAA GAU CdTdT-3′), VEGFR-1B (ID no. 191; 5′-GGA AAA GGA AAA AAA GCA AdTdT-3′), VEGFR-1C (ID no. 192; 5′-GGA UCU AGU UCA GGU UCA AdTdT-3′), VEGFR-3 (ID no. 194; 5′-GGA UGA AGA CAU UUG AGG AdTdT-3′), and negative-control siRNA (sense, 5′-AGU ACU GCU UAC GAU ACG GdTdT-3′).

Article Title: Latent KSHV Infection of Endothelial Cells Induces Integrin Beta3 to Activate Angiogenic Phenotypes
Article Snippet: .. Transfection of siRNA siRNA specific to integrin β3 and negative-control oligonucleotides were designed and synthesized by Ambion (Austin, TX). .. The following oligonucleotide sequences were used: integrin β3 (Ambion identification [ID] no. 112581; sense, 5′-GCU AAU UCU UUG ACC UGU UdTdT-3′ ) and negative-control siRNA (sense, 5′-AGU ACU GCU UAC GAU ACG GdTdT-3′ ).

Article Title: Kaposi's Sarcoma-Associated Herpesvirus Downregulates Transforming Growth Factor β2 To Promote Enhanced Stability of Capillary-Like Tube Formation
Article Snippet: .. Small interfering RNA (siRNA) specific to gp130 and negative-control oligonucleotides were designed and synthesized by Ambion (Austin, TX). .. The following oligonucleotide sequences were used: sense oligonucleotide 5′-GGC AUA CCU UAA ACA AGC UdTdT-3′ for gp130 (Ambion identification no. 106709) and sense oligonucleotide 5′-AGU ACU GCU UAC GAU ACG GdTdT-3′ for negative-control siRNA.

Synthesized:

Article Title: Ets-1 Is Required for the Activation of VEGFR3 during Latent Kaposi's Sarcoma-Associated Herpesvirus Infection of Endothelial Cells
Article Snippet: .. siRNAs specific to gp130, Ets-1, and negative-control oligonucleotides were designed and synthesized by Ambion (Austin, TX). .. The following oligonucleotide sequences were used: gp130 (Ambion identification [ID] no. 106709; sense, 5′-GGC AUA CCU UAA ACA AGC UdTdT-3′), Ets-1 (ID no. 146635; sense, 5′-GCA UAG AGA GCU ACG AUA GdTdT-3′), and negative-control siRNA (sense, 5′-AGU ACU GCU UAC GAU ACG GdTdT-3′).

Article Title: MicroRNA 452 Regulates Cell Proliferation, Cell Migration, and Angiogenesis in Colorectal Cancer by Suppressing VEGFA Expression
Article Snippet: .. The MIR452 mimic (hsa-miR-452, pre-miR miRNA precursor AM17100, product ID: PM12946) and negative control oligonucleotides were commercially synthesized (Ambion, Austin, TX, USA), and used at 50 nmol/mL for transfections. .. The transfections were performed with Lipofectamine RNAiMAX (Invitrogen) or siPORT NeoFX transfection agent (Ambion) according to the manufacturers’ recommendations.

Article Title: Activation of Akt through gp130 Receptor Signaling Is Required for Kaposi's Sarcoma-Associated Herpesvirus-Induced Lymphatic Reprogramming of Endothelial Cells ▿
Article Snippet: .. siRNA specific to gp130, Akt1, STAT3, Prox1, and negative-control oligonucleotides were designed and synthesized by Ambion (Austin, TX). .. The following oligonucleotide sequences were used: gp130 (Ambion identification [ID] no. 106709; sense, 5′-GGC AUA CCU UAA ACA AGC UdTdT-3′), Akt1 (ID no. 633; sense, 5′-GGG CAC UUU CGG CAA GGU GdTdT-3′), STAT3 (ID no. 116558; sense 5′-GCA CAA UCU ACG AAG AAU CdTdT-3′), Prox1 (ID no. 106879; sense, 5′-GGG AAU UUG UUA ACG AUG CdTdT-3′), VEGFR-1A (ID no. 190; 5′-GGU UCA AAA UUA AAA GAU CdTdT-3′), VEGFR-1B (ID no. 191; 5′-GGA AAA GGA AAA AAA GCA AdTdT-3′), VEGFR-1C (ID no. 192; 5′-GGA UCU AGU UCA GGU UCA AdTdT-3′), VEGFR-3 (ID no. 194; 5′-GGA UGA AGA CAU UUG AGG AdTdT-3′), and negative-control siRNA (sense, 5′-AGU ACU GCU UAC GAU ACG GdTdT-3′).

Article Title: Latent KSHV Infection of Endothelial Cells Induces Integrin Beta3 to Activate Angiogenic Phenotypes
Article Snippet: .. Transfection of siRNA siRNA specific to integrin β3 and negative-control oligonucleotides were designed and synthesized by Ambion (Austin, TX). .. The following oligonucleotide sequences were used: integrin β3 (Ambion identification [ID] no. 112581; sense, 5′-GCU AAU UCU UUG ACC UGU UdTdT-3′ ) and negative-control siRNA (sense, 5′-AGU ACU GCU UAC GAU ACG GdTdT-3′ ).

Article Title: Kaposi's Sarcoma-Associated Herpesvirus Downregulates Transforming Growth Factor β2 To Promote Enhanced Stability of Capillary-Like Tube Formation
Article Snippet: .. Small interfering RNA (siRNA) specific to gp130 and negative-control oligonucleotides were designed and synthesized by Ambion (Austin, TX). .. The following oligonucleotide sequences were used: sense oligonucleotide 5′-GGC AUA CCU UAA ACA AGC UdTdT-3′ for gp130 (Ambion identification no. 106709) and sense oligonucleotide 5′-AGU ACU GCU UAC GAU ACG GdTdT-3′ for negative-control siRNA.

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  • 92
    Thermo Fisher bugz sirna oligonucleotide
    Effects of <t>BuGZ</t> on AurA kinase activity in mitosis. (A–D) Cells were transfected with control, BuGZ, or TPX2 <t>siRNA</t> (si) or were treated with AurA inhibitor MLN8237 (100 nM) followed by staining with antibodies to total AurA (A) or p-AurA (B). Bars, 10 µm. The immunostaining intensity of total AurA (C) and p-AurA (D) in control siRNA–treated cells in metaphase or prometaphase (ProM, containing misaligned chromosomes or thick chromosome bars) and cells in the experimental groups containing misaligned chromosomes were quantified. 75–152 total cells from three independent experiments in each experiment were measured and quantified. Error bars indicate SEM. One-way ANOVA: *, P
    Bugz Sirna Oligonucleotide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bugz sirna oligonucleotide/product/Thermo Fisher
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bugz sirna oligonucleotide - by Bioz Stars, 2020-05
    92/100 stars
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    92
    Thermo Fisher oligonucleotide zrv1
    Identification of a cbrB-crcZ co-transcript in A . vinelandii . A. Representation of the cbrB-crcZ locus in A . vinelandii . The positions of the promoters is indicated, along with the oligonucleotides used for the reverse transcription-PCR (RT-PCR) assay of panel B. B. Identification of crcZ transcripts by RT-PCR. The wild-type strain AEIV was cultured in BAG medium with or without ammonium (NH 4 + ), under acetate (Ac) or glucose (Gl) consumption. The total RNA was purified and used to generate cDNA using an oligonucleotide annealing within crcZ <t>(Zrv1).</t> The cDNA was PCR amplified with primer pairs Zfw2/Zrv1 or Zfw1/Zrv1, generating products of 330 and 100 bp, respectively. Control reactions using genomic DNA (lanes 1 and 7) or RT-PCR reactions in the absence of cDNA (lanes 2 and 8) are shown. M, DNA Molecular Weight Marker. C. The activity of the cbrB promoter ( PcbrB ) is RpoN-independent. Strain JG513 ( PcbrB - gusA ; wt) and its rpoN - derivative AErpoNBgus, were cultured in BAG-N medium. Cells were harvested under acetate (5h) or glucose growing conditions (15 h), and the activity of ß-glucuronidase (ß-Gluc) was determined. D. Sensitivity of crcZ transcripts from the wild type strain AEIV (wt) or its isogenic rpoN - mutant to the TEX enzyme. crcZ RT-PCR reactions were performed as in panel B, using primers pair Zfw1/Zrv1, and total RNA extracted from cells grown in BAG-N medium for 5h. When indicated, prior to the generation of the cDNA, RNA samples were treated with TEX. The amount of cDNA in nanograms used as a template for the PCR reaction in each experimental condition is indicated at the top. RT-PCR reactions using 20 nanograms of cDNA derived from the gyrA mRNA was used as an internal control, using primer pair gyrAfw/gyrArev (100 bp amplicon). Control reactions using genomic DNA (C+) or RT-PCR reactions in the absence of cDNA (C-) are shown. M, DNA molecular weight marker. The assay was repeated twice obtaining essentially the same results.
    Oligonucleotide Zrv1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligonucleotide zrv1/product/Thermo Fisher
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    oligonucleotide zrv1 - by Bioz Stars, 2020-05
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    92
    Thermo Fisher l glama primer
    Molecular detection of S. <t>aucheniae</t> DNA by semi-nested PCR. Fragments of S. aucheniae 18S rRNA gene were amplified by semi-nested PCR from DNA samples extracted from a S. aucheniae macrocyst using the protocol described in Martín et al. (2016) (lane 1, amplicon size ∼400 bp) or in the present work (lane 2, amplicon size ∼550 bp). A duplex format of this assay was set up, including primers to amplify a ∼257 bp fragment of Lama <t>glama</t> DNA isolated from blood of llama. Lanes 3 and 4 show a positive and a negative result, respectively. M: 1 kb Plus DNA marker.
    L Glama Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l glama primer/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l glama primer - by Bioz Stars, 2020-05
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    99
    Thermo Fisher sirna oligonucleotides
    RASSF1A regulation is dependent on FoxM1. <t>T84</t> and Colo 205 cells were transfected with plasmid for overexpression of FoxM1. ( A ) Cell lysates were analyzed by immunoblot and quantified by densitometry for expression of FoxM1, RASSF1A. Expression is normalized against GAPDH. The right panel of ( A ) represents the densitometric analysis of FoxM1 and RASSF1A. ( B ) T84 and Colo 205 cells were transfected with <t>siRNA</t> for FoxM1 or control siRNA for 48 h. Cell lysates were evaluated for FoxM1 (( B ), 1st lane), RASSF1A (( B ), 2nd lane) by immunoblot and quantified by densitometry. The results are from three independent experiments. (** p
    Sirna Oligonucleotides, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 703 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sirna oligonucleotides/product/Thermo Fisher
    Average 99 stars, based on 703 article reviews
    Price from $9.99 to $1999.99
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    Effects of BuGZ on AurA kinase activity in mitosis. (A–D) Cells were transfected with control, BuGZ, or TPX2 siRNA (si) or were treated with AurA inhibitor MLN8237 (100 nM) followed by staining with antibodies to total AurA (A) or p-AurA (B). Bars, 10 µm. The immunostaining intensity of total AurA (C) and p-AurA (D) in control siRNA–treated cells in metaphase or prometaphase (ProM, containing misaligned chromosomes or thick chromosome bars) and cells in the experimental groups containing misaligned chromosomes were quantified. 75–152 total cells from three independent experiments in each experiment were measured and quantified. Error bars indicate SEM. One-way ANOVA: *, P

    Journal: The Journal of Cell Biology

    Article Title: Aurora A activation in mitosis promoted by BuGZ

    doi: 10.1083/jcb.201706103

    Figure Lengend Snippet: Effects of BuGZ on AurA kinase activity in mitosis. (A–D) Cells were transfected with control, BuGZ, or TPX2 siRNA (si) or were treated with AurA inhibitor MLN8237 (100 nM) followed by staining with antibodies to total AurA (A) or p-AurA (B). Bars, 10 µm. The immunostaining intensity of total AurA (C) and p-AurA (D) in control siRNA–treated cells in metaphase or prometaphase (ProM, containing misaligned chromosomes or thick chromosome bars) and cells in the experimental groups containing misaligned chromosomes were quantified. 75–152 total cells from three independent experiments in each experiment were measured and quantified. Error bars indicate SEM. One-way ANOVA: *, P

    Article Snippet: For BuGZ knockdown, we used our previously published BuGZ siRNA oligonucleotide (5′-GCCUGCUACACUUACAACAACUAGU-3′; , ), control oligonucleotide (12935-300; Thermo Fisher Scientific), and a TPX2 oligonucleotide (SI02665082; QIAGEN).

    Techniques: Activity Assay, Transfection, Staining, Immunostaining

    Identification of a cbrB-crcZ co-transcript in A . vinelandii . A. Representation of the cbrB-crcZ locus in A . vinelandii . The positions of the promoters is indicated, along with the oligonucleotides used for the reverse transcription-PCR (RT-PCR) assay of panel B. B. Identification of crcZ transcripts by RT-PCR. The wild-type strain AEIV was cultured in BAG medium with or without ammonium (NH 4 + ), under acetate (Ac) or glucose (Gl) consumption. The total RNA was purified and used to generate cDNA using an oligonucleotide annealing within crcZ (Zrv1). The cDNA was PCR amplified with primer pairs Zfw2/Zrv1 or Zfw1/Zrv1, generating products of 330 and 100 bp, respectively. Control reactions using genomic DNA (lanes 1 and 7) or RT-PCR reactions in the absence of cDNA (lanes 2 and 8) are shown. M, DNA Molecular Weight Marker. C. The activity of the cbrB promoter ( PcbrB ) is RpoN-independent. Strain JG513 ( PcbrB - gusA ; wt) and its rpoN - derivative AErpoNBgus, were cultured in BAG-N medium. Cells were harvested under acetate (5h) or glucose growing conditions (15 h), and the activity of ß-glucuronidase (ß-Gluc) was determined. D. Sensitivity of crcZ transcripts from the wild type strain AEIV (wt) or its isogenic rpoN - mutant to the TEX enzyme. crcZ RT-PCR reactions were performed as in panel B, using primers pair Zfw1/Zrv1, and total RNA extracted from cells grown in BAG-N medium for 5h. When indicated, prior to the generation of the cDNA, RNA samples were treated with TEX. The amount of cDNA in nanograms used as a template for the PCR reaction in each experimental condition is indicated at the top. RT-PCR reactions using 20 nanograms of cDNA derived from the gyrA mRNA was used as an internal control, using primer pair gyrAfw/gyrArev (100 bp amplicon). Control reactions using genomic DNA (C+) or RT-PCR reactions in the absence of cDNA (C-) are shown. M, DNA molecular weight marker. The assay was repeated twice obtaining essentially the same results.

    Journal: PLoS ONE

    Article Title: Expression of the sRNAs CrcZ and CrcY modulate the strength of carbon catabolite repression under diazotrophic or non-diazotrophic growing conditions in Azotobacter vinelandii

    doi: 10.1371/journal.pone.0208975

    Figure Lengend Snippet: Identification of a cbrB-crcZ co-transcript in A . vinelandii . A. Representation of the cbrB-crcZ locus in A . vinelandii . The positions of the promoters is indicated, along with the oligonucleotides used for the reverse transcription-PCR (RT-PCR) assay of panel B. B. Identification of crcZ transcripts by RT-PCR. The wild-type strain AEIV was cultured in BAG medium with or without ammonium (NH 4 + ), under acetate (Ac) or glucose (Gl) consumption. The total RNA was purified and used to generate cDNA using an oligonucleotide annealing within crcZ (Zrv1). The cDNA was PCR amplified with primer pairs Zfw2/Zrv1 or Zfw1/Zrv1, generating products of 330 and 100 bp, respectively. Control reactions using genomic DNA (lanes 1 and 7) or RT-PCR reactions in the absence of cDNA (lanes 2 and 8) are shown. M, DNA Molecular Weight Marker. C. The activity of the cbrB promoter ( PcbrB ) is RpoN-independent. Strain JG513 ( PcbrB - gusA ; wt) and its rpoN - derivative AErpoNBgus, were cultured in BAG-N medium. Cells were harvested under acetate (5h) or glucose growing conditions (15 h), and the activity of ß-glucuronidase (ß-Gluc) was determined. D. Sensitivity of crcZ transcripts from the wild type strain AEIV (wt) or its isogenic rpoN - mutant to the TEX enzyme. crcZ RT-PCR reactions were performed as in panel B, using primers pair Zfw1/Zrv1, and total RNA extracted from cells grown in BAG-N medium for 5h. When indicated, prior to the generation of the cDNA, RNA samples were treated with TEX. The amount of cDNA in nanograms used as a template for the PCR reaction in each experimental condition is indicated at the top. RT-PCR reactions using 20 nanograms of cDNA derived from the gyrA mRNA was used as an internal control, using primer pair gyrAfw/gyrArev (100 bp amplicon). Control reactions using genomic DNA (C+) or RT-PCR reactions in the absence of cDNA (C-) are shown. M, DNA molecular weight marker. The assay was repeated twice obtaining essentially the same results.

    Article Snippet: Thereafter, generation of cDNA by reverse transcription was conducted using 200 ng of RNA, the reverse oligonucleotide Zrv1 and the RevertAid H Minus Reverse Transcriptase (Thermo Scientific), as instructed by the manufacturer.

    Techniques: Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Cell Culture, Purification, Amplification, Molecular Weight, Marker, Activity Assay, Mutagenesis, Derivative Assay

    Molecular detection of S. aucheniae DNA by semi-nested PCR. Fragments of S. aucheniae 18S rRNA gene were amplified by semi-nested PCR from DNA samples extracted from a S. aucheniae macrocyst using the protocol described in Martín et al. (2016) (lane 1, amplicon size ∼400 bp) or in the present work (lane 2, amplicon size ∼550 bp). A duplex format of this assay was set up, including primers to amplify a ∼257 bp fragment of Lama glama DNA isolated from blood of llama. Lanes 3 and 4 show a positive and a negative result, respectively. M: 1 kb Plus DNA marker.

    Journal: Heliyon

    Article Title: Detection of Sarcocystis aucheniae in blood of llama using a duplex semi-nested PCR assay and its association with cyst infestation

    doi: 10.1016/j.heliyon.2018.e00928

    Figure Lengend Snippet: Molecular detection of S. aucheniae DNA by semi-nested PCR. Fragments of S. aucheniae 18S rRNA gene were amplified by semi-nested PCR from DNA samples extracted from a S. aucheniae macrocyst using the protocol described in Martín et al. (2016) (lane 1, amplicon size ∼400 bp) or in the present work (lane 2, amplicon size ∼550 bp). A duplex format of this assay was set up, including primers to amplify a ∼257 bp fragment of Lama glama DNA isolated from blood of llama. Lanes 3 and 4 show a positive and a negative result, respectively. M: 1 kb Plus DNA marker.

    Article Snippet: Both reactions were carried out in a final volume of 13 μL, containing 0.4 μM of each S. aucheniae primer and 0.1 μM of each L. glama primer, 0.2 mM of each dNTP (Thermo Fisher Scientific), 0.4 U GoTaq polymerase and its corresponding buffer (Promega, Madison, USA), and as template, 1 μL genomic DNA sample described in section in the first round, or the amplicon of the first round for secondary amplification.

    Techniques: Nested PCR, Amplification, Isolation, Marker

    RASSF1A regulation is dependent on FoxM1. T84 and Colo 205 cells were transfected with plasmid for overexpression of FoxM1. ( A ) Cell lysates were analyzed by immunoblot and quantified by densitometry for expression of FoxM1, RASSF1A. Expression is normalized against GAPDH. The right panel of ( A ) represents the densitometric analysis of FoxM1 and RASSF1A. ( B ) T84 and Colo 205 cells were transfected with siRNA for FoxM1 or control siRNA for 48 h. Cell lysates were evaluated for FoxM1 (( B ), 1st lane), RASSF1A (( B ), 2nd lane) by immunoblot and quantified by densitometry. The results are from three independent experiments. (** p

    Journal: Cancers

    Article Title: Identification of Cross Talk between FoxM1 and RASSF1A as a Therapeutic Target of Colon Cancer

    doi: 10.3390/cancers11020199

    Figure Lengend Snippet: RASSF1A regulation is dependent on FoxM1. T84 and Colo 205 cells were transfected with plasmid for overexpression of FoxM1. ( A ) Cell lysates were analyzed by immunoblot and quantified by densitometry for expression of FoxM1, RASSF1A. Expression is normalized against GAPDH. The right panel of ( A ) represents the densitometric analysis of FoxM1 and RASSF1A. ( B ) T84 and Colo 205 cells were transfected with siRNA for FoxM1 or control siRNA for 48 h. Cell lysates were evaluated for FoxM1 (( B ), 1st lane), RASSF1A (( B ), 2nd lane) by immunoblot and quantified by densitometry. The results are from three independent experiments. (** p

    Article Snippet: T84 and Colo 205 cells were transfected with siRNA oligonucleotides using TurboFect (Thermo Scientific, #R0533) according to the manufacturer’s recommendations.

    Techniques: Transfection, Plasmid Preparation, Over Expression, Expressing