oligofectamine transfection lipid  (Thermo Fisher)


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    Oligofectamine Transfection Reagent
    Description:
    Oligofectamine Transfection Reagent is a proprietary formulation for transfecting oligonucleotides and short interfering RNA siRNA into eukaryotic cells Oligofectamine Transfection Reagent forms stable complexes with oligos permitting efficient transfection into eukaryotic cells in a highly specific yet nontoxic fashion Oligofectamine Reagent is suitable for nuclear and cytoplasmic targets and transfects a wide variety of cell lines including CHO HEK 293 NIH 3T3 and HeLa Using Oligofectamine Transfection ReagentOligofectamine Reagent is easy to use because it provides a simple and fast protocol Just dilute Oligofectamine Reagent mix with oligonucleotide and add to your cells Oligofectamine Reagent requires nanomolar quantities of antisense oligonucleotide reducing the amount of valuable oligonucleotide needed by up to a thousand fold This makes it ideal for high throughput applications In addition Oligofectamine Reagent has also been shown to work for siRNA transfections We recommend this reagent when performing RNAi knockdown experiments in HeLa cells Please visit RNAi Central for more information
    Catalog Number:
    12252011
    Price:
    None
    Applications:
    Cell Culture|RNAi Transfection|Stem Cell & Primary Cell Transfections|Synthetic siRNA Transfection|Transfection
    Category:
    Cell Culture Transfection Reagents
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    Structured Review

    Thermo Fisher oligofectamine transfection lipid
    Oligofectamine Transfection Reagent is a proprietary formulation for transfecting oligonucleotides and short interfering RNA siRNA into eukaryotic cells Oligofectamine Transfection Reagent forms stable complexes with oligos permitting efficient transfection into eukaryotic cells in a highly specific yet nontoxic fashion Oligofectamine Reagent is suitable for nuclear and cytoplasmic targets and transfects a wide variety of cell lines including CHO HEK 293 NIH 3T3 and HeLa Using Oligofectamine Transfection ReagentOligofectamine Reagent is easy to use because it provides a simple and fast protocol Just dilute Oligofectamine Reagent mix with oligonucleotide and add to your cells Oligofectamine Reagent requires nanomolar quantities of antisense oligonucleotide reducing the amount of valuable oligonucleotide needed by up to a thousand fold This makes it ideal for high throughput applications In addition Oligofectamine Reagent has also been shown to work for siRNA transfections We recommend this reagent when performing RNAi knockdown experiments in HeLa cells Please visit RNAi Central for more information
    https://www.bioz.com/result/oligofectamine transfection lipid/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    oligofectamine transfection lipid - by Bioz Stars, 2020-12
    99/100 stars

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    Concentration Assay:

    Article Title: Role of the TAB2-related protein TAB3 in IL-1 and TNF signaling
    Article Snippet: .. HeLa cells (1 × 105 cells/well) were seeded into six-well (35 mm) plates to 20–30% confluency, and transfection of the RNA oligonucleotides was performed using Oligofectamine (Invitrogen) to a final RNA concentration of 400 nM. .. The cells were harvested at different time points post-treatment with IL-1β (10 ng/ml) or TNFα (10 ng/ml) and lysed for use in western blot analysis.

    Article Title: HIV-1 Vpr-Induced Apoptosis Is Cell Cycle Dependent and Requires Bax but Not ANT
    Article Snippet: .. Smart pool siRNAs were transfected at a final concentration of 100 nM into exponentially growing HeLa cells with Oligofectamine (Invitrogen) according to the manufacturer's protocol. ..

    Article Title: MiR-24 Tumor Suppressor Activity Is Regulated Independent of p53 and through a Target Site Polymorphism
    Article Snippet: .. Transfections of miR-24 and siRNAs RKO, HT-29, U2-OS, MG63, HCT-116 (wt-p53) and HCT-116 (null-p53) cells (2×105 ) were plated in six-well plates and transfected with 100 nM of either miR-24 or non-specific miRNA (Ambion) after 24 h by Oligofectamine (Invitrogen) according to the manufacturer's protocols. siRNA against DHFR was purchased from Dharmacon and transfected with Oligofectamine (Invitrogen) at a final concentration of 100 nM. .. RNA Isolation Total RNA, including miRNA, was isolated from the miR-24 transfected cell lines (24 h after transfection) and from clinical colorectal cancer samples using TRIzol reagent, according to the manufacturer's instructions (Invitrogen).

    Transfection:

    Article Title: Role of the TAB2-related protein TAB3 in IL-1 and TNF signaling
    Article Snippet: .. HeLa cells (1 × 105 cells/well) were seeded into six-well (35 mm) plates to 20–30% confluency, and transfection of the RNA oligonucleotides was performed using Oligofectamine (Invitrogen) to a final RNA concentration of 400 nM. .. The cells were harvested at different time points post-treatment with IL-1β (10 ng/ml) or TNFα (10 ng/ml) and lysed for use in western blot analysis.

    Article Title: HIV-1 Vpr-Induced Apoptosis Is Cell Cycle Dependent and Requires Bax but Not ANT
    Article Snippet: .. Smart pool siRNAs were transfected at a final concentration of 100 nM into exponentially growing HeLa cells with Oligofectamine (Invitrogen) according to the manufacturer's protocol. ..

    Article Title: Effect of 2′-5′/3′-5′ phosphodiester linkage heterogeneity on RNA interference
    Article Snippet: .. Subsequently, cells were washed once with serum-free DMEM media and then 80 μl of serum-free DMEM media was added. siRNA and control duplexes were diluted up to 20 μl with serum-free media and transfection reagent (Oligofectamine, Invitrogen) and added to the appropriate well (for a total of 100 μl) at increasing concentrations (0.16, 0.8, 4, 20 and 100 nM). .. Cells were incubated overnight (for a total of 24 h post-transfection).

    Article Title: Identification of Cytoskeleton-Associated Proteins Essential for Lysosomal Stability and Survival of Human Cancer Cells
    Article Snippet: .. Cells were transfected with single (8 nM) or pooled siRNAs (3×6.7 nM) using Oligofectamine (Invitrogen). .. CTSB siRNA (described previously ), Hsp70 (HSPA1) siRNA and non-targeting siRNA (both described previously ) as well as AllStar Negative Control siRNA (QIAGEN) were used as controls.

    Article Title: A systems biology analysis of the changes in gene expression via silencing of HPV-18 E1 expression in HeLa cells
    Article Snippet: .. For transfection, 4 µl of Oligofectamine and 100 nM siRNA were diluted in Opti-MEM I reduced-serum medium (Invitrogen) in a final volume of 200 µl. .. Cultured cells in 0.8 ml Opti-MEM I were transfected with a mixed solution of siRNA, and 4 h later the culture was supplemented with 0.5 ml MEM and l (+)-glutamine containing 30% fetal bovine serum.

    Article Title: MiR-24 Tumor Suppressor Activity Is Regulated Independent of p53 and through a Target Site Polymorphism
    Article Snippet: .. Transfections of miR-24 and siRNAs RKO, HT-29, U2-OS, MG63, HCT-116 (wt-p53) and HCT-116 (null-p53) cells (2×105 ) were plated in six-well plates and transfected with 100 nM of either miR-24 or non-specific miRNA (Ambion) after 24 h by Oligofectamine (Invitrogen) according to the manufacturer's protocols. siRNA against DHFR was purchased from Dharmacon and transfected with Oligofectamine (Invitrogen) at a final concentration of 100 nM. .. RNA Isolation Total RNA, including miRNA, was isolated from the miR-24 transfected cell lines (24 h after transfection) and from clinical colorectal cancer samples using TRIzol reagent, according to the manufacturer's instructions (Invitrogen).

    Article Title: The role of YY1 in reduced HP1? gene expression in invasive human breast cancer cells
    Article Snippet: .. The following day the cells were transfected with siRNA using Oligofectamine reagent (Invitrogen, Carlsbad, CA, USA). siRNA oligos were diluted to 500 nM with Opti-MEM (Invitrogen, Carlsbad, CA, USA). ..

    Cell Culture:

    Article Title: Electrophysiological Correlates of Hyperalgesic Priming In Vitro and In Vivo
    Article Snippet: .. Total RNA from cultured dorsal root ganglia treated with 1 μM of the phosphothioate antisense / mismatch oligonucleotides for 60 h in the presence of oligofectamine™ (Invitrogen) was extracted using Trizol reagent (Invitrogen) with the PureLink™ RNA mini kit (Ambion) according to the manufactures instructions. .. The amount of RNA was quantified with a spectrophotometer (UV-160, Shimadzu) and cDNA preparation was carried out with 1 μg of total RNA/sample and the superscript III platinum one-step RT-PCR system (Invitrogen).

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    Thermo Fisher hela cells
    Mps1 destabilizes kinetochore-microtubule attachments by phosphorylating the hinge region of the Ska complex ). Inset displays a magnified view of the hinge region, with serine 34 on Ska3 labeled in red. (B) <t>HeLa</t> cells bearing the indicated LAP-Ska3 transgenes were <t>transfected</t> with a 3′ UTR-specific siRNA ( siSKA3 ) or luciferase-specific siRNA ( siGL2 ) as a negative control, then harvested 72 hr later and analyzed by Western blotting. Chk1 was used a loading control. Results are representative of two experiments. (C) HeLa cells transgenic for LAP-Ska3 were synchronized in mitosis with nocodazole and harvested immediately (wt, S34A, and S34D; lanes 1–3), or after further treatment with MG132 (wt + MG, lane 4) or MG132 and reversine (wt + rev, lane 5) for 2 hours. LAP-Ska3 was precipitated with S-protein-agarose and analyzed by Western blotting to assess its phosphorylation state and binding to Ska1. Results are representative of three independent experiments. (D) SKA3 -depleted HeLa cells were treated with nocodazole for 2 hr and analyzed by IFM to detect LAP-Ska3 (green) or CREST (red). Images are representative of three experiments. Kinetochore targeting of LAP-Ska3 is reported as a percentage ± SEM of the wildtype control. (E and F) Control and SKA3 -depleted HeLa cells were treated with MG132 for 50 minutes, incubated on ice for 10 minutes, and analyzed by IFM as shown. Images shown are representative of three experiments. Cold-stable microtubules were quantified from 8 to 12 cells per condition and compared using one-way ANOVA and Kruskal-Wallis post hoc test. No cold-stability defect was observed in the absence of Ska3 depletion, indicating that the S34D allele acts recessively.
    Hela Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 25657 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mps1 destabilizes kinetochore-microtubule attachments by phosphorylating the hinge region of the Ska complex ). Inset displays a magnified view of the hinge region, with serine 34 on Ska3 labeled in red. (B) HeLa cells bearing the indicated LAP-Ska3 transgenes were transfected with a 3′ UTR-specific siRNA ( siSKA3 ) or luciferase-specific siRNA ( siGL2 ) as a negative control, then harvested 72 hr later and analyzed by Western blotting. Chk1 was used a loading control. Results are representative of two experiments. (C) HeLa cells transgenic for LAP-Ska3 were synchronized in mitosis with nocodazole and harvested immediately (wt, S34A, and S34D; lanes 1–3), or after further treatment with MG132 (wt + MG, lane 4) or MG132 and reversine (wt + rev, lane 5) for 2 hours. LAP-Ska3 was precipitated with S-protein-agarose and analyzed by Western blotting to assess its phosphorylation state and binding to Ska1. Results are representative of three independent experiments. (D) SKA3 -depleted HeLa cells were treated with nocodazole for 2 hr and analyzed by IFM to detect LAP-Ska3 (green) or CREST (red). Images are representative of three experiments. Kinetochore targeting of LAP-Ska3 is reported as a percentage ± SEM of the wildtype control. (E and F) Control and SKA3 -depleted HeLa cells were treated with MG132 for 50 minutes, incubated on ice for 10 minutes, and analyzed by IFM as shown. Images shown are representative of three experiments. Cold-stable microtubules were quantified from 8 to 12 cells per condition and compared using one-way ANOVA and Kruskal-Wallis post hoc test. No cold-stability defect was observed in the absence of Ska3 depletion, indicating that the S34D allele acts recessively.

    Journal: Developmental cell

    Article Title: Mps1 regulates kinetochore-microtubule attachment stability via the Ska complex to ensure error-free chromosome segregation

    doi: 10.1016/j.devcel.2017.03.025

    Figure Lengend Snippet: Mps1 destabilizes kinetochore-microtubule attachments by phosphorylating the hinge region of the Ska complex ). Inset displays a magnified view of the hinge region, with serine 34 on Ska3 labeled in red. (B) HeLa cells bearing the indicated LAP-Ska3 transgenes were transfected with a 3′ UTR-specific siRNA ( siSKA3 ) or luciferase-specific siRNA ( siGL2 ) as a negative control, then harvested 72 hr later and analyzed by Western blotting. Chk1 was used a loading control. Results are representative of two experiments. (C) HeLa cells transgenic for LAP-Ska3 were synchronized in mitosis with nocodazole and harvested immediately (wt, S34A, and S34D; lanes 1–3), or after further treatment with MG132 (wt + MG, lane 4) or MG132 and reversine (wt + rev, lane 5) for 2 hours. LAP-Ska3 was precipitated with S-protein-agarose and analyzed by Western blotting to assess its phosphorylation state and binding to Ska1. Results are representative of three independent experiments. (D) SKA3 -depleted HeLa cells were treated with nocodazole for 2 hr and analyzed by IFM to detect LAP-Ska3 (green) or CREST (red). Images are representative of three experiments. Kinetochore targeting of LAP-Ska3 is reported as a percentage ± SEM of the wildtype control. (E and F) Control and SKA3 -depleted HeLa cells were treated with MG132 for 50 minutes, incubated on ice for 10 minutes, and analyzed by IFM as shown. Images shown are representative of three experiments. Cold-stable microtubules were quantified from 8 to 12 cells per condition and compared using one-way ANOVA and Kruskal-Wallis post hoc test. No cold-stability defect was observed in the absence of Ska3 depletion, indicating that the S34D allele acts recessively.

    Article Snippet: Synthetic siRNAs were transfected into HeLa cells using Oligofectamine (ThermoFisher) and into hTERT-RPE1 cells using RNAiMax (ThermoFisher).

    Techniques: Labeling, Transfection, Luciferase, Negative Control, Western Blot, Transgenic Assay, Binding Assay, Incubation

    Regulated phosphorylation and dephosphorylation of the Ska complex hinge are required for accurate chromosome segregation (A) HeLa cells transgenic for LAP-Ska3 and H2B-RFP were transfected with siRNAs and followed by spinning disk microscopy. Time relative to nuclear envelope breakdown (NEBD) is indicated on each image and representative of at least three experiments per condition. (B) The length of M phase (from NEBD to mitotic exit or cell death) was quantified from 104 to 208 cells in three experiments and compared using one-way ANOVA and Kruskal-Wallis post hoc test. (C) Cells analyzed in (B) were also scored for their eventual fates (persistent mitotic arrest, cell death, or anaphase with or without lagging chromosomes). (D and E) HeLa cells reconstituted with wildtype or mutant LAP-Ska3 were treated with monastrol for 2 hours. Following washout, cells were incubated in MG132 for 60 minutes, then fixed and analyzed by IFM. Images are representative of two experiments (n = 40 to 100 cells analyzed per condition; P -values computed using Fisher’s exact test). (F and G) Live imaging of LAP-Ska3 and H2B-RFP in cells reaching metaphase after endogenous SKA3 depletion. Inter-kinetochore distances were measured from 105 to 130 sister kinetochore pairs (6 to 10 cells per condition) and compared using one-way ANOVA and Kruskal-Wallis post hoc test.

    Journal: Developmental cell

    Article Title: Mps1 regulates kinetochore-microtubule attachment stability via the Ska complex to ensure error-free chromosome segregation

    doi: 10.1016/j.devcel.2017.03.025

    Figure Lengend Snippet: Regulated phosphorylation and dephosphorylation of the Ska complex hinge are required for accurate chromosome segregation (A) HeLa cells transgenic for LAP-Ska3 and H2B-RFP were transfected with siRNAs and followed by spinning disk microscopy. Time relative to nuclear envelope breakdown (NEBD) is indicated on each image and representative of at least three experiments per condition. (B) The length of M phase (from NEBD to mitotic exit or cell death) was quantified from 104 to 208 cells in three experiments and compared using one-way ANOVA and Kruskal-Wallis post hoc test. (C) Cells analyzed in (B) were also scored for their eventual fates (persistent mitotic arrest, cell death, or anaphase with or without lagging chromosomes). (D and E) HeLa cells reconstituted with wildtype or mutant LAP-Ska3 were treated with monastrol for 2 hours. Following washout, cells were incubated in MG132 for 60 minutes, then fixed and analyzed by IFM. Images are representative of two experiments (n = 40 to 100 cells analyzed per condition; P -values computed using Fisher’s exact test). (F and G) Live imaging of LAP-Ska3 and H2B-RFP in cells reaching metaphase after endogenous SKA3 depletion. Inter-kinetochore distances were measured from 105 to 130 sister kinetochore pairs (6 to 10 cells per condition) and compared using one-way ANOVA and Kruskal-Wallis post hoc test.

    Article Snippet: Synthetic siRNAs were transfected into HeLa cells using Oligofectamine (ThermoFisher) and into hTERT-RPE1 cells using RNAiMax (ThermoFisher).

    Techniques: De-Phosphorylation Assay, Transgenic Assay, Transfection, Microscopy, Mutagenesis, Incubation, Imaging

    The acid-induced Cl - current is significantly reduced by TRPM7 siRNA. (A) Amplification plot of ß-actin (black), TRPM7 (orange), and TRPM6 (violet) mRNA in MDCT cells (RT-qPCR; n = 3, triplicate). (B) Relative expression of TRPM7 to TRPM6 using the data shown in A. (C) Comparison of TRPM7 (black) and TRPM6 (grey) mRNA after 24 h transfection with scramble or TRPM7 siRNA, normalized to ß-actin (RT-qPCR; n = 4). (D) Representative MDCT current at pH 7.4 (oI) after transfection with scramble (black) or TRPM7 (grey) siRNA. (E) Mean current density of MDCT cells transfected with scramble (n = 10) or TRPM7 (n = 9) siRNA at -60 mV and pH 7.4. (F) Mean current density of MDCT cells transfected with scramble (n = 10) or TRPM7 (n = 9) siRNA at +100 mV and pH 7.4. (G) Representative MDCT current at pH 5.0 (oI) after transfection with scramble (black) or TRPM7 (grey) siRNA. (H) Mean current density of MDCT cells transfected with scramble (n = 8) or TRPM7 (n = 9) siRNA at -60 mV and pH 5.0. (I) Mean current density of MDCT cells transfected with scramble (n = 8) or TRPM7 (n = 9) siRNA at +100 mV and pH 5.0. Quantified mRNA was statistically compared by a Mann-Whitney U test (mean ± SEM). Currents were statistically compared via unpaired two-tailed Student's t -tests (mean ± SEM). Data were considered significant when p

    Journal: Biochimica et Biophysica Acta

    Article Title: Characterization of constitutive and acid-induced outwardly rectifying chloride currents in immortalized mouse distal tubular cells

    doi: 10.1016/j.bbagen.2017.05.004

    Figure Lengend Snippet: The acid-induced Cl - current is significantly reduced by TRPM7 siRNA. (A) Amplification plot of ß-actin (black), TRPM7 (orange), and TRPM6 (violet) mRNA in MDCT cells (RT-qPCR; n = 3, triplicate). (B) Relative expression of TRPM7 to TRPM6 using the data shown in A. (C) Comparison of TRPM7 (black) and TRPM6 (grey) mRNA after 24 h transfection with scramble or TRPM7 siRNA, normalized to ß-actin (RT-qPCR; n = 4). (D) Representative MDCT current at pH 7.4 (oI) after transfection with scramble (black) or TRPM7 (grey) siRNA. (E) Mean current density of MDCT cells transfected with scramble (n = 10) or TRPM7 (n = 9) siRNA at -60 mV and pH 7.4. (F) Mean current density of MDCT cells transfected with scramble (n = 10) or TRPM7 (n = 9) siRNA at +100 mV and pH 7.4. (G) Representative MDCT current at pH 5.0 (oI) after transfection with scramble (black) or TRPM7 (grey) siRNA. (H) Mean current density of MDCT cells transfected with scramble (n = 8) or TRPM7 (n = 9) siRNA at -60 mV and pH 5.0. (I) Mean current density of MDCT cells transfected with scramble (n = 8) or TRPM7 (n = 9) siRNA at +100 mV and pH 5.0. Quantified mRNA was statistically compared by a Mann-Whitney U test (mean ± SEM). Currents were statistically compared via unpaired two-tailed Student's t -tests (mean ± SEM). Data were considered significant when p

    Article Snippet: MDCT cells were plated in a 35 mm plastic cell culture dish, transfected with 100 nM siRNA using oligofectamine (ThermoFisher Scientific Invitrogen) for 6 h in OPTIMEM (ThermoFisher Scientific Gibco), and utilized 24 h after transfection.

    Techniques: Amplification, Quantitative RT-PCR, Expressing, Transfection, MANN-WHITNEY, Two Tailed Test

    Expression of ARSB and ARSG. ( a ) The efficiency of ARSB gene silencing in PASM and HPAEC cells using siRNA; ( b ) expression of ARSG transcript in PASM and HPAEC cells with a depleted ARSB gene. Results are shown as mean ± SD from three biological experiments. *—statistically significant changes (compared to controls treated with non-targeting siRNA; p -value

    Journal: International Journal of Molecular Sciences

    Article Title: A Possible Role for Arylsulfatase G in Dermatan Sulfate Metabolism

    doi: 10.3390/ijms21144913

    Figure Lengend Snippet: Expression of ARSB and ARSG. ( a ) The efficiency of ARSB gene silencing in PASM and HPAEC cells using siRNA; ( b ) expression of ARSG transcript in PASM and HPAEC cells with a depleted ARSB gene. Results are shown as mean ± SD from three biological experiments. *—statistically significant changes (compared to controls treated with non-targeting siRNA; p -value

    Article Snippet: Gene SilencingThe silencing of the ARSB gene was achieved using a siRNA (Thermo Scientific, Waltham, MA, USA) and oligofectamine reagent (Life Technologies, Grand Island, NY, USA) according to the manufacturer’s protocols.

    Techniques: Expressing

    Depletion of Separase in HeLa cells. (A) A scheme depicting the strategy used to generate Separase-depleted S phase arrested and cycling cells. (B) Immunoblot analysis of Pericentrin cleavage after Separase depletion. Please note reduced cleavage of Pericentrin in mitotic Separase-depleted cells. The signal after longer exposure is included to ascertain the lack of Pericentrin cleavage in S phase arrested cells by Hydroxyurea (HU) after Plk1TD expression. (C) Immunofluorescence analysis of cell population transfected with non-targeting or Separase siRNA. Separase labeling (magenta) is significantly reduced in the population of cycling cells. DNA is labeled in blue. (D) Centrosome-associated Pericentrin levels are higher in Separase-depleted postmitotic cells in comparison with cells with detectable Separase (ND cells). DNA bridges connecting two sister cells (DB) are the hallmark of Separase-depleted cells and are visible in cells lacking Separase. (E) Two sister cells from cell culture depleted for Separase associated with DNA bridge. Pericentrin is still abundant on centrosomes. STORM analysis of centrosomes #1, shows its distribution around centrioles. Scale bars: 10 μm and 1μm for for centriole inserts.

    Journal: bioRxiv

    Article Title: Procentriole microtubules as drivers of centriole reduplication

    doi: 10.1101/2020.04.12.038307

    Figure Lengend Snippet: Depletion of Separase in HeLa cells. (A) A scheme depicting the strategy used to generate Separase-depleted S phase arrested and cycling cells. (B) Immunoblot analysis of Pericentrin cleavage after Separase depletion. Please note reduced cleavage of Pericentrin in mitotic Separase-depleted cells. The signal after longer exposure is included to ascertain the lack of Pericentrin cleavage in S phase arrested cells by Hydroxyurea (HU) after Plk1TD expression. (C) Immunofluorescence analysis of cell population transfected with non-targeting or Separase siRNA. Separase labeling (magenta) is significantly reduced in the population of cycling cells. DNA is labeled in blue. (D) Centrosome-associated Pericentrin levels are higher in Separase-depleted postmitotic cells in comparison with cells with detectable Separase (ND cells). DNA bridges connecting two sister cells (DB) are the hallmark of Separase-depleted cells and are visible in cells lacking Separase. (E) Two sister cells from cell culture depleted for Separase associated with DNA bridge. Pericentrin is still abundant on centrosomes. STORM analysis of centrosomes #1, shows its distribution around centrioles. Scale bars: 10 μm and 1μm for for centriole inserts.

    Article Snippet: Cells, growing on glass coverslips or in 75 cm2 cell culture flasks, were transfected 2h after shake off with 300 nM of siRNA oligonucleotides and Oligofectamine (Thermo Fisher Scientific; 12252011).

    Techniques: Expressing, Immunofluorescence, Transfection, Labeling, Cell Culture