oligofectamine lipid transfection reagent  (Thermo Fisher)


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    Name:
    Oligofectamine Transfection Reagent
    Description:
    Oligofectamine Transfection Reagent is a proprietary formulation for transfecting oligonucleotides and short interfering RNA siRNA into eukaryotic cells Oligofectamine Transfection Reagent forms stable complexes with oligos permitting efficient transfection into eukaryotic cells in a highly specific yet nontoxic fashion Oligofectamine Reagent is suitable for nuclear and cytoplasmic targets and transfects a wide variety of cell lines including CHO HEK 293 NIH 3T3 and HeLa Using Oligofectamine Transfection ReagentOligofectamine Reagent is easy to use because it provides a simple and fast protocol Just dilute Oligofectamine Reagent mix with oligonucleotide and add to your cells Oligofectamine Reagent requires nanomolar quantities of antisense oligonucleotide reducing the amount of valuable oligonucleotide needed by up to a thousand fold This makes it ideal for high throughput applications In addition Oligofectamine Reagent has also been shown to work for siRNA transfections We recommend this reagent when performing RNAi knockdown experiments in HeLa cells Please visit RNAi Central for more information
    Catalog Number:
    12252011
    Price:
    None
    Applications:
    Cell Culture|RNAi Transfection|Stem Cell & Primary Cell Transfections|Synthetic siRNA Transfection|Transfection
    Category:
    Cell Culture Transfection Reagents
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    Structured Review

    Thermo Fisher oligofectamine lipid transfection reagent
    Oligofectamine Transfection Reagent is a proprietary formulation for transfecting oligonucleotides and short interfering RNA siRNA into eukaryotic cells Oligofectamine Transfection Reagent forms stable complexes with oligos permitting efficient transfection into eukaryotic cells in a highly specific yet nontoxic fashion Oligofectamine Reagent is suitable for nuclear and cytoplasmic targets and transfects a wide variety of cell lines including CHO HEK 293 NIH 3T3 and HeLa Using Oligofectamine Transfection ReagentOligofectamine Reagent is easy to use because it provides a simple and fast protocol Just dilute Oligofectamine Reagent mix with oligonucleotide and add to your cells Oligofectamine Reagent requires nanomolar quantities of antisense oligonucleotide reducing the amount of valuable oligonucleotide needed by up to a thousand fold This makes it ideal for high throughput applications In addition Oligofectamine Reagent has also been shown to work for siRNA transfections We recommend this reagent when performing RNAi knockdown experiments in HeLa cells Please visit RNAi Central for more information
    https://www.bioz.com/result/oligofectamine lipid transfection reagent/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    oligofectamine lipid transfection reagent - by Bioz Stars, 2020-07
    99/100 stars

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    Transfection:

    Article Title: Rottlerin enhances IL-1?-induced COX-2 expression through sustained p38 MAPK activation in MDA-MB-231 human breast cancer cells
    Article Snippet: .. Cells were transfected with siRNA oligonucleotides using Oligofectamine™ Reagent (Invitrogen). .. Abad MJ, Bessa AL, Ballarin B, Aragon O, Gonzales E, Bermejo P. Anti-inflammatory activity of four Bolivian Baccharis species (Compositae) J Ethnopharmacol.

    Article Title: ?Np63α regulates keratinocyte proliferation by controlling PTEN expression and localization
    Article Snippet: .. The ΔNp63 α and CA-Akt plasmids used were described previously., H1299 cells were transfected using Lipofectamine 2000, and A431, HaCaT and HEK cells were transfected with two rounds of siRNA using Oligofectamine as per the manufacturer's instructions (Invitrogen, Carlsband, CA, USA). .. Cells were harvested for immunoblot and qRT-PCR studies 24 h after the second transfection.

    Article Title: HIV-1 Vpr-Mediated G2 Arrest Involves the DDB1-CUL4AVPRBP E3 Ubiquitin Ligase
    Article Snippet: .. siRNA-mediated protein knockdown. siRNA targeting VPRBP (siGenome SMARTpool M-021119–00) and scrambled control siRNA (non-targeting siRNA #2) were obtained from Dharmacon ( http://www.dharmacon.com/ ). siRNA was transfected using Oligofectamine (Invitrogen Canada) according to the manufacturer instructions. .. Briefly, 300 pmol of siRNA were pre-incubated with 15 μl of Oligofectamine and overlayed on cells at 50% confluence (the final concentration of siRNA was 125 nM).

    Article Title: Identification of Cytoskeleton-Associated Proteins Essential for Lysosomal Stability and Survival of Human Cancer Cells
    Article Snippet: .. Cells were transfected with single (8 nM) or pooled siRNAs (3×6.7 nM) using Oligofectamine (Invitrogen). .. CTSB siRNA (described previously ), Hsp70 (HSPA1) siRNA and non-targeting siRNA (both described previously ) as well as AllStar Negative Control siRNA (QIAGEN) were used as controls.

    Article Title: A systems biology analysis of the changes in gene expression via silencing of HPV-18 E1 expression in HeLa cells
    Article Snippet: .. For transfection, 4 µl of Oligofectamine and 100 nM siRNA were diluted in Opti-MEM I reduced-serum medium (Invitrogen) in a final volume of 200 µl. .. Cultured cells in 0.8 ml Opti-MEM I were transfected with a mixed solution of siRNA, and 4 h later the culture was supplemented with 0.5 ml MEM and l (+)-glutamine containing 30% fetal bovine serum.

    Article Title: Auxilin Depletion Causes Self-Assembly of Clathrin into Membraneless Cages In Vivo
    Article Snippet: .. HeLa cells were transfected with siRNA using Oligofectamine (Invitrogen), as specified by the manufacturer. ..

    Article Title: FOXM1 is overexpressed in B-acute lymphoblastic leukemia (B-ALL) and its inhibition sensitizes B-ALL cells to chemotherapeutic drugs
    Article Snippet: .. RNA interference with small interfering RNAs (siRNAs) For FOXM1 silencing, REH or NALM-6 cells were transiently transfected with siRNA SMARTpool reagents purchased from Thermo Scientific Dharmacon (Lafayette, CO, USA) using the transfection reagent Oligofectamine (Life Technologies, UK) according to the manufacturer's instructions. ..

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    Thermo Fisher sirna
    The acid-induced Cl - current is significantly reduced by TRPM7 <t>siRNA.</t> (A) Amplification plot of ß-actin (black), TRPM7 (orange), and TRPM6 (violet) mRNA in MDCT cells (RT-qPCR; n = 3, triplicate). (B) Relative expression of TRPM7 to TRPM6 using the data shown in A. (C) Comparison of TRPM7 (black) and TRPM6 (grey) mRNA after 24 h transfection with scramble or TRPM7 siRNA, normalized to ß-actin (RT-qPCR; n = 4). (D) Representative MDCT current at pH 7.4 (oI) after transfection with scramble (black) or TRPM7 (grey) siRNA. (E) Mean current density of MDCT cells <t>transfected</t> with scramble (n = 10) or TRPM7 (n = 9) siRNA at -60 mV and pH 7.4. (F) Mean current density of MDCT cells transfected with scramble (n = 10) or TRPM7 (n = 9) siRNA at +100 mV and pH 7.4. (G) Representative MDCT current at pH 5.0 (oI) after transfection with scramble (black) or TRPM7 (grey) siRNA. (H) Mean current density of MDCT cells transfected with scramble (n = 8) or TRPM7 (n = 9) siRNA at -60 mV and pH 5.0. (I) Mean current density of MDCT cells transfected with scramble (n = 8) or TRPM7 (n = 9) siRNA at +100 mV and pH 5.0. Quantified mRNA was statistically compared by a Mann-Whitney U test (mean ± SEM). Currents were statistically compared via unpaired two-tailed Student's t -tests (mean ± SEM). Data were considered significant when p
    Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 23609 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sirna/product/Thermo Fisher
    Average 99 stars, based on 23609 article reviews
    Price from $9.99 to $1999.99
    sirna - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    The acid-induced Cl - current is significantly reduced by TRPM7 siRNA. (A) Amplification plot of ß-actin (black), TRPM7 (orange), and TRPM6 (violet) mRNA in MDCT cells (RT-qPCR; n = 3, triplicate). (B) Relative expression of TRPM7 to TRPM6 using the data shown in A. (C) Comparison of TRPM7 (black) and TRPM6 (grey) mRNA after 24 h transfection with scramble or TRPM7 siRNA, normalized to ß-actin (RT-qPCR; n = 4). (D) Representative MDCT current at pH 7.4 (oI) after transfection with scramble (black) or TRPM7 (grey) siRNA. (E) Mean current density of MDCT cells transfected with scramble (n = 10) or TRPM7 (n = 9) siRNA at -60 mV and pH 7.4. (F) Mean current density of MDCT cells transfected with scramble (n = 10) or TRPM7 (n = 9) siRNA at +100 mV and pH 7.4. (G) Representative MDCT current at pH 5.0 (oI) after transfection with scramble (black) or TRPM7 (grey) siRNA. (H) Mean current density of MDCT cells transfected with scramble (n = 8) or TRPM7 (n = 9) siRNA at -60 mV and pH 5.0. (I) Mean current density of MDCT cells transfected with scramble (n = 8) or TRPM7 (n = 9) siRNA at +100 mV and pH 5.0. Quantified mRNA was statistically compared by a Mann-Whitney U test (mean ± SEM). Currents were statistically compared via unpaired two-tailed Student's t -tests (mean ± SEM). Data were considered significant when p

    Journal: Biochimica et Biophysica Acta

    Article Title: Characterization of constitutive and acid-induced outwardly rectifying chloride currents in immortalized mouse distal tubular cells

    doi: 10.1016/j.bbagen.2017.05.004

    Figure Lengend Snippet: The acid-induced Cl - current is significantly reduced by TRPM7 siRNA. (A) Amplification plot of ß-actin (black), TRPM7 (orange), and TRPM6 (violet) mRNA in MDCT cells (RT-qPCR; n = 3, triplicate). (B) Relative expression of TRPM7 to TRPM6 using the data shown in A. (C) Comparison of TRPM7 (black) and TRPM6 (grey) mRNA after 24 h transfection with scramble or TRPM7 siRNA, normalized to ß-actin (RT-qPCR; n = 4). (D) Representative MDCT current at pH 7.4 (oI) after transfection with scramble (black) or TRPM7 (grey) siRNA. (E) Mean current density of MDCT cells transfected with scramble (n = 10) or TRPM7 (n = 9) siRNA at -60 mV and pH 7.4. (F) Mean current density of MDCT cells transfected with scramble (n = 10) or TRPM7 (n = 9) siRNA at +100 mV and pH 7.4. (G) Representative MDCT current at pH 5.0 (oI) after transfection with scramble (black) or TRPM7 (grey) siRNA. (H) Mean current density of MDCT cells transfected with scramble (n = 8) or TRPM7 (n = 9) siRNA at -60 mV and pH 5.0. (I) Mean current density of MDCT cells transfected with scramble (n = 8) or TRPM7 (n = 9) siRNA at +100 mV and pH 5.0. Quantified mRNA was statistically compared by a Mann-Whitney U test (mean ± SEM). Currents were statistically compared via unpaired two-tailed Student's t -tests (mean ± SEM). Data were considered significant when p

    Article Snippet: MDCT cells were plated in a 35 mm plastic cell culture dish, transfected with 100 nM siRNA using oligofectamine (ThermoFisher Scientific Invitrogen) for 6 h in OPTIMEM (ThermoFisher Scientific Gibco), and utilized 24 h after transfection.

    Techniques: Amplification, Quantitative RT-PCR, Expressing, Transfection, MANN-WHITNEY, Two Tailed Test

    Mimic-1 inhibits colon cancer cell proliferation in HCT116, RKO, SW480, and SW620 colon cancer cell lines with and without transfection reagents (A) HCT116, RKO, SW480, and SW620 colon cancer cells were transfected with negative control miRNA, native miR-129, or Mimic-1 at the concentration of 50 nM using oligofectamine, and cell proliferation was measured by WST-1 assay (B) HCT116, RKO, SW480, and SW620 colon cancer cells were transfected with negative control miRNA, native miR-129, or Mimic-1 at the concentration of 50 nM without any transfection reagent, and cell proliferation was measured with WST-1 assay. (C) qRT-PCR analysis of Mimic-1 levels in colon cancer cells without transfection reagent. (D) Without transfection reagent, Mimic-1 expression reduced expression of E2F3. ( ** p

    Journal: Oncotarget

    Article Title: Development of novel miR-129 mimics with enhanced efficacy to eliminate chemoresistant colon cancer stem cells

    doi: 10.18632/oncotarget.22322

    Figure Lengend Snippet: Mimic-1 inhibits colon cancer cell proliferation in HCT116, RKO, SW480, and SW620 colon cancer cell lines with and without transfection reagents (A) HCT116, RKO, SW480, and SW620 colon cancer cells were transfected with negative control miRNA, native miR-129, or Mimic-1 at the concentration of 50 nM using oligofectamine, and cell proliferation was measured by WST-1 assay (B) HCT116, RKO, SW480, and SW620 colon cancer cells were transfected with negative control miRNA, native miR-129, or Mimic-1 at the concentration of 50 nM without any transfection reagent, and cell proliferation was measured with WST-1 assay. (C) qRT-PCR analysis of Mimic-1 levels in colon cancer cells without transfection reagent. (D) Without transfection reagent, Mimic-1 expression reduced expression of E2F3. ( ** p

    Article Snippet: For Transfection, cells (1×105 ) were plated in six-well plates and transfected with 50 nM of either mir-129 precursor, non-specific miRNA (Thermo Fischer) or modified miR-129 mimics (Dharmacon, GE Life Sciences) after 24 hours using Oligofectamine (Thermo Fischer) following the manufacturer’s protocols.

    Techniques: Transfection, Negative Control, Concentration Assay, WST-1 Assay, Quantitative RT-PCR, Expressing

    Regulation of FA disassembly requires SOCS6 interaction with CRL5 and Cas. Microtubule-dependent FA disassembly was assayed in transfected HeLa cells, scoring the percent of cells lacking FAs at 30 min after nocodazole reversal. ( a ) Rescue of normal FA disassembly by expression of siRNA-resistant mouse SOCS6 wildtype (WT) but not by SOCS box mutant LCQQ. ( b ) Expression of CasFF, a mutant that does not bind SOCS6, stimulates FA disassembly independent of Cul5, while wildtype Cas only stimulates disassembly when Cul5 is absent and Cas15F, which is unable to bind downstream signaling molecules, is inactive. Mean and standard deviation of two experiments. DOI: http://dx.doi.org/10.7554/eLife.17440.016

    Journal: eLife

    Article Title: The ubiquitin-proteasome system regulates focal adhesions at the leading edge of migrating cells

    doi: 10.7554/eLife.17440

    Figure Lengend Snippet: Regulation of FA disassembly requires SOCS6 interaction with CRL5 and Cas. Microtubule-dependent FA disassembly was assayed in transfected HeLa cells, scoring the percent of cells lacking FAs at 30 min after nocodazole reversal. ( a ) Rescue of normal FA disassembly by expression of siRNA-resistant mouse SOCS6 wildtype (WT) but not by SOCS box mutant LCQQ. ( b ) Expression of CasFF, a mutant that does not bind SOCS6, stimulates FA disassembly independent of Cul5, while wildtype Cas only stimulates disassembly when Cul5 is absent and Cas15F, which is unable to bind downstream signaling molecules, is inactive. Mean and standard deviation of two experiments. DOI: http://dx.doi.org/10.7554/eLife.17440.016

    Article Snippet: Briefly, cells were transfected with 50 pmol pooled siRNA oligonucleotides using Oligofectamine (Thermo Fisher Scientific) on days 1 and 3, transferred to collagen IV-coated glass coverslips on day four and analyzed on day 5.

    Techniques: Transfection, Expressing, Mutagenesis, Standard Deviation

    The in vitro gene-silencing effect of exosomes loaded with small interfering ribonucleic acid (siRNA). Notes: The graphs depict the blocking effect of electroporated endothelial exosomes with siRNA(luc) ( A ) and siRNA(luc) complexed with Oligofectamine ( B ) on primary endothelial cells expressing luciferase ( P

    Journal: International Journal of Nanomedicine

    Article Title: In vitro evaluation of endothelial exosomes as carriers for small interfering ribonucleic acid delivery

    doi: 10.2147/IJN.S64267

    Figure Lengend Snippet: The in vitro gene-silencing effect of exosomes loaded with small interfering ribonucleic acid (siRNA). Notes: The graphs depict the blocking effect of electroporated endothelial exosomes with siRNA(luc) ( A ) and siRNA(luc) complexed with Oligofectamine ( B ) on primary endothelial cells expressing luciferase ( P

    Article Snippet: Luciferase-expressing endothelial cells seeded on 24-well plates (prepared as earlier) were transfected with a final concentration of 100 nM siRNA(luc) or siRNA(cont) complexed with 1.5 μL/well Oligofectamine (Thermo Fisher Scientific).

    Techniques: In Vitro, Blocking Assay, Expressing, Luciferase