oligofectamine lipid transfection reagent  (Thermo Fisher)


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    Name:
    Oligofectamine Transfection Reagent
    Description:
    Oligofectamine Transfection Reagent is a proprietary formulation for transfecting oligonucleotides and short interfering RNA siRNA into eukaryotic cells Oligofectamine Transfection Reagent forms stable complexes with oligos permitting efficient transfection into eukaryotic cells in a highly specific yet nontoxic fashion Oligofectamine Reagent is suitable for nuclear and cytoplasmic targets and transfects a wide variety of cell lines including CHO HEK 293 NIH 3T3 and HeLa Using Oligofectamine Transfection ReagentOligofectamine Reagent is easy to use because it provides a simple and fast protocol Just dilute Oligofectamine Reagent mix with oligonucleotide and add to your cells Oligofectamine Reagent requires nanomolar quantities of antisense oligonucleotide reducing the amount of valuable oligonucleotide needed by up to a thousand fold This makes it ideal for high throughput applications In addition Oligofectamine Reagent has also been shown to work for siRNA transfections We recommend this reagent when performing RNAi knockdown experiments in HeLa cells Please visit RNAi Central for more information
    Catalog Number:
    12252011
    Price:
    None
    Applications:
    Cell Culture|RNAi Transfection|Stem Cell & Primary Cell Transfections|Synthetic siRNA Transfection|Transfection
    Category:
    Cell Culture Transfection Reagents
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    Structured Review

    Thermo Fisher oligofectamine lipid transfection reagent
    Oligofectamine Transfection Reagent is a proprietary formulation for transfecting oligonucleotides and short interfering RNA siRNA into eukaryotic cells Oligofectamine Transfection Reagent forms stable complexes with oligos permitting efficient transfection into eukaryotic cells in a highly specific yet nontoxic fashion Oligofectamine Reagent is suitable for nuclear and cytoplasmic targets and transfects a wide variety of cell lines including CHO HEK 293 NIH 3T3 and HeLa Using Oligofectamine Transfection ReagentOligofectamine Reagent is easy to use because it provides a simple and fast protocol Just dilute Oligofectamine Reagent mix with oligonucleotide and add to your cells Oligofectamine Reagent requires nanomolar quantities of antisense oligonucleotide reducing the amount of valuable oligonucleotide needed by up to a thousand fold This makes it ideal for high throughput applications In addition Oligofectamine Reagent has also been shown to work for siRNA transfections We recommend this reagent when performing RNAi knockdown experiments in HeLa cells Please visit RNAi Central for more information
    https://www.bioz.com/result/oligofectamine lipid transfection reagent/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    oligofectamine lipid transfection reagent - by Bioz Stars, 2020-10
    99/100 stars

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    Concentration Assay:

    Article Title: HIV-1 Vpr-Induced Apoptosis Is Cell Cycle Dependent and Requires Bax but Not ANT
    Article Snippet: .. Smart pool siRNAs were transfected at a final concentration of 100 nM into exponentially growing HeLa cells with Oligofectamine (Invitrogen) according to the manufacturer's protocol. ..

    Article Title: MiR-24 Tumor Suppressor Activity Is Regulated Independent of p53 and through a Target Site Polymorphism
    Article Snippet: .. Transfections of miR-24 and siRNAs RKO, HT-29, U2-OS, MG63, HCT-116 (wt-p53) and HCT-116 (null-p53) cells (2×105 ) were plated in six-well plates and transfected with 100 nM of either miR-24 or non-specific miRNA (Ambion) after 24 h by Oligofectamine (Invitrogen) according to the manufacturer's protocols. siRNA against DHFR was purchased from Dharmacon and transfected with Oligofectamine (Invitrogen) at a final concentration of 100 nM. .. RNA Isolation Total RNA, including miRNA, was isolated from the miR-24 transfected cell lines (24 h after transfection) and from clinical colorectal cancer samples using TRIzol reagent, according to the manufacturer's instructions (Invitrogen).

    Transfection:

    Article Title: HIV-1 Vpr-Induced Apoptosis Is Cell Cycle Dependent and Requires Bax but Not ANT
    Article Snippet: .. Smart pool siRNAs were transfected at a final concentration of 100 nM into exponentially growing HeLa cells with Oligofectamine (Invitrogen) according to the manufacturer's protocol. ..

    Article Title: Clues to CD2-associated Protein Involvement in Cytokinesis D⃞V⃞
    Article Snippet: .. For CD2AP small interfering RNA (siRNA) transfections, 40–50% confluent HeLa cells cultivated in DMEM 10% fetal calf serum were used for transfection with Oligofectamine (Invitrogen Cergy Pontoise, France), as indicated by the manufacturer. .. Briefly, for each 35-mm dish, 10 μl of 10 μM CD2AP siRNA (sc-29984; Santa Cruz Biotechnology) was diluted into 175 μl of Opti-MEM I (Invitrogen SARL) and incubated for 5 min.

    Article Title: Effect of 2′-5′/3′-5′ phosphodiester linkage heterogeneity on RNA interference
    Article Snippet: .. Subsequently, cells were washed once with serum-free DMEM media and then 80 μl of serum-free DMEM media was added. siRNA and control duplexes were diluted up to 20 μl with serum-free media and transfection reagent (Oligofectamine, Invitrogen) and added to the appropriate well (for a total of 100 μl) at increasing concentrations (0.16, 0.8, 4, 20 and 100 nM). .. Cells were incubated overnight (for a total of 24 h post-transfection).

    Article Title: Identification of Cytoskeleton-Associated Proteins Essential for Lysosomal Stability and Survival of Human Cancer Cells
    Article Snippet: .. Cells were transfected with single (8 nM) or pooled siRNAs (3×6.7 nM) using Oligofectamine (Invitrogen). .. CTSB siRNA (described previously ), Hsp70 (HSPA1) siRNA and non-targeting siRNA (both described previously ) as well as AllStar Negative Control siRNA (QIAGEN) were used as controls.

    Article Title: MiR-24 Tumor Suppressor Activity Is Regulated Independent of p53 and through a Target Site Polymorphism
    Article Snippet: .. Transfections of miR-24 and siRNAs RKO, HT-29, U2-OS, MG63, HCT-116 (wt-p53) and HCT-116 (null-p53) cells (2×105 ) were plated in six-well plates and transfected with 100 nM of either miR-24 or non-specific miRNA (Ambion) after 24 h by Oligofectamine (Invitrogen) according to the manufacturer's protocols. siRNA against DHFR was purchased from Dharmacon and transfected with Oligofectamine (Invitrogen) at a final concentration of 100 nM. .. RNA Isolation Total RNA, including miRNA, was isolated from the miR-24 transfected cell lines (24 h after transfection) and from clinical colorectal cancer samples using TRIzol reagent, according to the manufacturer's instructions (Invitrogen).

    Article Title: The role of YY1 in reduced HP1? gene expression in invasive human breast cancer cells
    Article Snippet: .. The following day the cells were transfected with siRNA using Oligofectamine reagent (Invitrogen, Carlsbad, CA, USA). siRNA oligos were diluted to 500 nM with Opti-MEM (Invitrogen, Carlsbad, CA, USA). ..

    Article Title: Myc Enforces Overexpression of EZH2 in Early Prostatic Neoplasia via Transcriptional and Post-transcriptional Mechanisms
    Article Snippet: .. Transfections Cells were transfected using Oligofectamine or Lipofectamine 2000 (Invitrogen). .. MYC siRNA pools (Dharmacon, L-003282), siCONTROL Non-Targeting siRNA pool #1 (Dharmacon, D-001810), miRIDIAN Mimic hsa-mir-26a (Dharmacon, C-300499), miRIDIAN Mimic hsa-mir-26b (Dharmacon, C-300501) and miRIDIAN microRNA Mimic Negative Control #1 (Dharmacon CN-001000) were transfected at a final concentration of 50nM.

    Small Interfering RNA:

    Article Title: Clues to CD2-associated Protein Involvement in Cytokinesis D⃞V⃞
    Article Snippet: .. For CD2AP small interfering RNA (siRNA) transfections, 40–50% confluent HeLa cells cultivated in DMEM 10% fetal calf serum were used for transfection with Oligofectamine (Invitrogen Cergy Pontoise, France), as indicated by the manufacturer. .. Briefly, for each 35-mm dish, 10 μl of 10 μM CD2AP siRNA (sc-29984; Santa Cruz Biotechnology) was diluted into 175 μl of Opti-MEM I (Invitrogen SARL) and incubated for 5 min.

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    Thermo Fisher sirna oligonucleotides
    Depletion of Separase in HeLa cells. (A) A scheme depicting the strategy used to generate Separase-depleted S phase arrested and cycling cells. (B) Immunoblot analysis of Pericentrin cleavage after Separase depletion. Please note reduced cleavage of Pericentrin in mitotic Separase-depleted cells. The signal after longer exposure is included to ascertain the lack of Pericentrin cleavage in S phase arrested cells by Hydroxyurea (HU) after Plk1TD expression. (C) Immunofluorescence analysis of cell population <t>transfected</t> with non-targeting or Separase <t>siRNA.</t> Separase labeling (magenta) is significantly reduced in the population of cycling cells. DNA is labeled in blue. (D) Centrosome-associated Pericentrin levels are higher in Separase-depleted postmitotic cells in comparison with cells with detectable Separase (ND cells). DNA bridges connecting two sister cells (DB) are the hallmark of Separase-depleted cells and are visible in cells lacking Separase. (E) Two sister cells from cell culture depleted for Separase associated with DNA bridge. Pericentrin is still abundant on centrosomes. STORM analysis of centrosomes #1, shows its distribution around centrioles. Scale bars: 10 μm and 1μm for for centriole inserts.
    Sirna Oligonucleotides, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 19942 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sirna oligonucleotides/product/Thermo Fisher
    Average 99 stars, based on 19942 article reviews
    Price from $9.99 to $1999.99
    sirna oligonucleotides - by Bioz Stars, 2020-10
    99/100 stars
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    Depletion of Separase in HeLa cells. (A) A scheme depicting the strategy used to generate Separase-depleted S phase arrested and cycling cells. (B) Immunoblot analysis of Pericentrin cleavage after Separase depletion. Please note reduced cleavage of Pericentrin in mitotic Separase-depleted cells. The signal after longer exposure is included to ascertain the lack of Pericentrin cleavage in S phase arrested cells by Hydroxyurea (HU) after Plk1TD expression. (C) Immunofluorescence analysis of cell population transfected with non-targeting or Separase siRNA. Separase labeling (magenta) is significantly reduced in the population of cycling cells. DNA is labeled in blue. (D) Centrosome-associated Pericentrin levels are higher in Separase-depleted postmitotic cells in comparison with cells with detectable Separase (ND cells). DNA bridges connecting two sister cells (DB) are the hallmark of Separase-depleted cells and are visible in cells lacking Separase. (E) Two sister cells from cell culture depleted for Separase associated with DNA bridge. Pericentrin is still abundant on centrosomes. STORM analysis of centrosomes #1, shows its distribution around centrioles. Scale bars: 10 μm and 1μm for for centriole inserts.

    Journal: bioRxiv

    Article Title: Procentriole microtubules as drivers of centriole reduplication

    doi: 10.1101/2020.04.12.038307

    Figure Lengend Snippet: Depletion of Separase in HeLa cells. (A) A scheme depicting the strategy used to generate Separase-depleted S phase arrested and cycling cells. (B) Immunoblot analysis of Pericentrin cleavage after Separase depletion. Please note reduced cleavage of Pericentrin in mitotic Separase-depleted cells. The signal after longer exposure is included to ascertain the lack of Pericentrin cleavage in S phase arrested cells by Hydroxyurea (HU) after Plk1TD expression. (C) Immunofluorescence analysis of cell population transfected with non-targeting or Separase siRNA. Separase labeling (magenta) is significantly reduced in the population of cycling cells. DNA is labeled in blue. (D) Centrosome-associated Pericentrin levels are higher in Separase-depleted postmitotic cells in comparison with cells with detectable Separase (ND cells). DNA bridges connecting two sister cells (DB) are the hallmark of Separase-depleted cells and are visible in cells lacking Separase. (E) Two sister cells from cell culture depleted for Separase associated with DNA bridge. Pericentrin is still abundant on centrosomes. STORM analysis of centrosomes #1, shows its distribution around centrioles. Scale bars: 10 μm and 1μm for for centriole inserts.

    Article Snippet: Cells, growing on glass coverslips or in 75 cm2 cell culture flasks, were transfected 2h after shake off with 300 nM of siRNA oligonucleotides and Oligofectamine (Thermo Fisher Scientific; 12252011).

    Techniques: Expressing, Immunofluorescence, Transfection, Labeling, Cell Culture

    The acid-induced Cl - current is significantly reduced by TRPM7 siRNA. (A) Amplification plot of ß-actin (black), TRPM7 (orange), and TRPM6 (violet) mRNA in MDCT cells (RT-qPCR; n = 3, triplicate). (B) Relative expression of TRPM7 to TRPM6 using the data shown in A. (C) Comparison of TRPM7 (black) and TRPM6 (grey) mRNA after 24 h transfection with scramble or TRPM7 siRNA, normalized to ß-actin (RT-qPCR; n = 4). (D) Representative MDCT current at pH 7.4 (oI) after transfection with scramble (black) or TRPM7 (grey) siRNA. (E) Mean current density of MDCT cells transfected with scramble (n = 10) or TRPM7 (n = 9) siRNA at -60 mV and pH 7.4. (F) Mean current density of MDCT cells transfected with scramble (n = 10) or TRPM7 (n = 9) siRNA at +100 mV and pH 7.4. (G) Representative MDCT current at pH 5.0 (oI) after transfection with scramble (black) or TRPM7 (grey) siRNA. (H) Mean current density of MDCT cells transfected with scramble (n = 8) or TRPM7 (n = 9) siRNA at -60 mV and pH 5.0. (I) Mean current density of MDCT cells transfected with scramble (n = 8) or TRPM7 (n = 9) siRNA at +100 mV and pH 5.0. Quantified mRNA was statistically compared by a Mann-Whitney U test (mean ± SEM). Currents were statistically compared via unpaired two-tailed Student's t -tests (mean ± SEM). Data were considered significant when p

    Journal: Biochimica et Biophysica Acta

    Article Title: Characterization of constitutive and acid-induced outwardly rectifying chloride currents in immortalized mouse distal tubular cells

    doi: 10.1016/j.bbagen.2017.05.004

    Figure Lengend Snippet: The acid-induced Cl - current is significantly reduced by TRPM7 siRNA. (A) Amplification plot of ß-actin (black), TRPM7 (orange), and TRPM6 (violet) mRNA in MDCT cells (RT-qPCR; n = 3, triplicate). (B) Relative expression of TRPM7 to TRPM6 using the data shown in A. (C) Comparison of TRPM7 (black) and TRPM6 (grey) mRNA after 24 h transfection with scramble or TRPM7 siRNA, normalized to ß-actin (RT-qPCR; n = 4). (D) Representative MDCT current at pH 7.4 (oI) after transfection with scramble (black) or TRPM7 (grey) siRNA. (E) Mean current density of MDCT cells transfected with scramble (n = 10) or TRPM7 (n = 9) siRNA at -60 mV and pH 7.4. (F) Mean current density of MDCT cells transfected with scramble (n = 10) or TRPM7 (n = 9) siRNA at +100 mV and pH 7.4. (G) Representative MDCT current at pH 5.0 (oI) after transfection with scramble (black) or TRPM7 (grey) siRNA. (H) Mean current density of MDCT cells transfected with scramble (n = 8) or TRPM7 (n = 9) siRNA at -60 mV and pH 5.0. (I) Mean current density of MDCT cells transfected with scramble (n = 8) or TRPM7 (n = 9) siRNA at +100 mV and pH 5.0. Quantified mRNA was statistically compared by a Mann-Whitney U test (mean ± SEM). Currents were statistically compared via unpaired two-tailed Student's t -tests (mean ± SEM). Data were considered significant when p

    Article Snippet: MDCT cells were plated in a 35 mm plastic cell culture dish, transfected with 100 nM siRNA using oligofectamine (ThermoFisher Scientific Invitrogen) for 6 h in OPTIMEM (ThermoFisher Scientific Gibco), and utilized 24 h after transfection.

    Techniques: Amplification, Quantitative RT-PCR, Expressing, Transfection, MANN-WHITNEY, Two Tailed Test

    Expression of ARSB and ARSG. ( a ) The efficiency of ARSB gene silencing in PASM and HPAEC cells using siRNA; ( b ) expression of ARSG transcript in PASM and HPAEC cells with a depleted ARSB gene. Results are shown as mean ± SD from three biological experiments. *—statistically significant changes (compared to controls treated with non-targeting siRNA; p -value

    Journal: International Journal of Molecular Sciences

    Article Title: A Possible Role for Arylsulfatase G in Dermatan Sulfate Metabolism

    doi: 10.3390/ijms21144913

    Figure Lengend Snippet: Expression of ARSB and ARSG. ( a ) The efficiency of ARSB gene silencing in PASM and HPAEC cells using siRNA; ( b ) expression of ARSG transcript in PASM and HPAEC cells with a depleted ARSB gene. Results are shown as mean ± SD from three biological experiments. *—statistically significant changes (compared to controls treated with non-targeting siRNA; p -value

    Article Snippet: Gene SilencingThe silencing of the ARSB gene was achieved using a siRNA (Thermo Scientific, Waltham, MA, USA) and oligofectamine reagent (Life Technologies, Grand Island, NY, USA) according to the manufacturer’s protocols.

    Techniques: Expressing

    SPIRE1/2 interact with active Rab27a via their membrane-binding C-termini. a A schematic representation of the domain structure of SPIRE1/2, Mlph and truncations used in interaction studies ( b , c , e , f ). Interaction of SPIRE1/2 and Rab27a was investigated using GST pull-down ( b , c , e , f ) and BiFC ( d ) assays (see Experimental procedures). b , c , e Western blots and Ponceau S stained filters showing the results of pull-down assays measuring the interaction of GST-Rab27a (active Q78L and inactive T23N mutants) with SPIRE1/2 and the indicated truncations (Myc-tagged ( b , c ) and GFP-tagged ( e ) in vitro). c is a contrast enhanced version of the section of b showing interaction of SPIRE2 with Rab27a-Q78L and Rab27a-T23N. d Fluorescence images and a bee swarm plot (upper and lower panels) showing the results of the BiFC assay, reporting the interaction of Rab27a with SPIRE1 and SPIRE2 in HEK293a cells (see Experimental procedures). Images of mCherry indicate transfection efficiency and vYFP indicates BiFC, i.e., interaction. The bee swarm plot shows the BiFC signal for populations of cells expressing SPIRE1/2 with and without active and inactive Rab27a mutants. Data shown are from three independent experiments. ****, *** and ** indicate significant differences of p =

    Journal: Nature Communications

    Article Title: Rab27a co-ordinates actin-dependent transport by controlling organelle-associated motors and track assembly proteins

    doi: 10.1038/s41467-020-17212-6

    Figure Lengend Snippet: SPIRE1/2 interact with active Rab27a via their membrane-binding C-termini. a A schematic representation of the domain structure of SPIRE1/2, Mlph and truncations used in interaction studies ( b , c , e , f ). Interaction of SPIRE1/2 and Rab27a was investigated using GST pull-down ( b , c , e , f ) and BiFC ( d ) assays (see Experimental procedures). b , c , e Western blots and Ponceau S stained filters showing the results of pull-down assays measuring the interaction of GST-Rab27a (active Q78L and inactive T23N mutants) with SPIRE1/2 and the indicated truncations (Myc-tagged ( b , c ) and GFP-tagged ( e ) in vitro). c is a contrast enhanced version of the section of b showing interaction of SPIRE2 with Rab27a-Q78L and Rab27a-T23N. d Fluorescence images and a bee swarm plot (upper and lower panels) showing the results of the BiFC assay, reporting the interaction of Rab27a with SPIRE1 and SPIRE2 in HEK293a cells (see Experimental procedures). Images of mCherry indicate transfection efficiency and vYFP indicates BiFC, i.e., interaction. The bee swarm plot shows the BiFC signal for populations of cells expressing SPIRE1/2 with and without active and inactive Rab27a mutants. Data shown are from three independent experiments. ****, *** and ** indicate significant differences of p =

    Article Snippet: Cultures of immortal melan-f, melan-ash, melan-ln and melan-a melanocytes, and HEK293a were maintained, infected with adenovirus expression vectors and transfected with siRNA oligonucleotides at a final concentration of 66.7 fmol/μl of transfection mixture and Oligofectamine transfection reagent diluted 300-fold in optiMEM medium (both Thermo-Fisher) , .

    Techniques: Binding Assay, Bimolecular Fluorescence Complementation Assay, Western Blot, Staining, In Vitro, Fluorescence, Transfection, Expressing

    The FMN interaction (KIND) and AF nucleation (WH2) domains of SPIRE1 are essential for melanosome dispersion. a A schematic representation of the domain structure of human SPIRE1, and the correspondence with mutant and chimeric proteins used in functional studies ( b , c ). Numbers indicate amino acid boundaries. K KIND, W WH2, M GTBM globular tail domain binding motif, S SB SPIRE box, F FYVE-type zinc finger, H C-terminal flanking sequences similar to H2 of Slp/Slac-proteins. b melan-a cells were depleted of SPIRE1/2 by siRNA transfection and 72 h later infected with adenoviruses expressing the indicated proteins. Cells were fixed 24 h later, processed for immunofluorescence and imaged using bright-field and fluorescence optics to observe melanosome and protein distribution/expression (see Experimental procedures). Scale bars = 100, 20 and 3 μm in low, medium and high-magnification images. Boxes in KW-Rab27a images indicate the region shown below. For the merged image green = KW-Rab27a and magenta = melanosomes. c Is a bee swarm plot showing the percentage of human SPIRE1/2 expressing SPIRE1/2-depleted melan-a cells (50 cells for each condition in each experiment), in which melanosomes are classed as dispersed and/or hyper-dispersed. Results shown are from of three independent experiments. Source data for c are provided in the Supplementary Source data file.

    Journal: Nature Communications

    Article Title: Rab27a co-ordinates actin-dependent transport by controlling organelle-associated motors and track assembly proteins

    doi: 10.1038/s41467-020-17212-6

    Figure Lengend Snippet: The FMN interaction (KIND) and AF nucleation (WH2) domains of SPIRE1 are essential for melanosome dispersion. a A schematic representation of the domain structure of human SPIRE1, and the correspondence with mutant and chimeric proteins used in functional studies ( b , c ). Numbers indicate amino acid boundaries. K KIND, W WH2, M GTBM globular tail domain binding motif, S SB SPIRE box, F FYVE-type zinc finger, H C-terminal flanking sequences similar to H2 of Slp/Slac-proteins. b melan-a cells were depleted of SPIRE1/2 by siRNA transfection and 72 h later infected with adenoviruses expressing the indicated proteins. Cells were fixed 24 h later, processed for immunofluorescence and imaged using bright-field and fluorescence optics to observe melanosome and protein distribution/expression (see Experimental procedures). Scale bars = 100, 20 and 3 μm in low, medium and high-magnification images. Boxes in KW-Rab27a images indicate the region shown below. For the merged image green = KW-Rab27a and magenta = melanosomes. c Is a bee swarm plot showing the percentage of human SPIRE1/2 expressing SPIRE1/2-depleted melan-a cells (50 cells for each condition in each experiment), in which melanosomes are classed as dispersed and/or hyper-dispersed. Results shown are from of three independent experiments. Source data for c are provided in the Supplementary Source data file.

    Article Snippet: Cultures of immortal melan-f, melan-ash, melan-ln and melan-a melanocytes, and HEK293a were maintained, infected with adenovirus expression vectors and transfected with siRNA oligonucleotides at a final concentration of 66.7 fmol/μl of transfection mixture and Oligofectamine transfection reagent diluted 300-fold in optiMEM medium (both Thermo-Fisher) , .

    Techniques: Mutagenesis, Functional Assay, Binding Assay, Transfection, Infection, Expressing, Immunofluorescence, Fluorescence