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Boehringer Mannheim oligodeoxythymidylic acid primers
Oligodeoxythymidylic Acid Primers, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oligodeoxythymidylic acid primers/product/Boehringer Mannheim
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
oligodeoxythymidylic acid primers - by Bioz Stars, 2020-08
85/100 stars

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Polymerase Chain Reaction:

Article Title: Dual Transforming Activities of the FUS (TLS)-ERG Leukemia Fusion Protein Conferred by Two N-Terminal Domains of FUS (TLS)
Article Snippet: .. PCR-amplification was carried out between the same oligo(dT) primers and arbitrary 10-mers (Operon Technologies) by using Taq DNA polymerase (Boehringer Mannheim) with 1 cycle of denaturation at 94°C for 3 min, annealing at 40°C for 5 min, and extension at 72°C for 5 min followed by 24 cycles of denaturation at 95°C for 15 s, annealing at 40°C for 2 min, and extension at 72°C for 1 min. .. The PCR products were separated by gel electrophoresis in 6% polyacrylamide gels, stained with SYBR green I (Molecular Probes), and detected with FluorImager SI (Molecular Dynamics).

other:

Article Title: Cytokines and soluble cytokine receptor induction after IL-12 administration in cancer patients
Article Snippet: Total cellular RNA (2 μg) were mixed with oligo-dT primers to synthesize cDNA (First strand cDNA synthesis kit; Boehringer-Mannheim, Mannheim, Germany) according to the manufacturer's protocol.

Article Title: PACAP type I receptor activation regulates ECL cells and gastric acid secretion
Article Snippet: Total RNA (5 μg) from enriched rat ECL cells was used to synthesize cDNA by reverse transcription with oligo-dT primers (Boehringer-Mannheim).

Cell Culture:

Article Title: Defective Development of ?/? T Cells in Interleukin 7 Receptor-Deficient Mice Is Due to Impaired Expression of T Cell Receptor ? Genes
Article Snippet: .. Reverse transcription (RT) of 1–2 μg of total RNA from sorted (and cultured) thymocytes was performed using oligo-dT primers and avian reverse transcriptase (both from Boehringer Mannheim). .. Each sorting experiment used pooled thymocytes from four to eight IL-7Rα−/− mice for the analysis of transgene expression and pooled thymocytes from three to six B6 mice for the analysis of endogenous TCR-γ gene expression in α/β cells. cDNA was serially diluted 3- or 4-fold, and PCR was performed for 28 cycles with the addition of 1.0 μCi of [α-32 P]dCTP (Amersham Pharmacia Biotech) per sample in a total reaction volume of 50 μl.

Synthesized:

Article Title: Pleiotrophin Induces Transdifferentiation of Monocytes Into Functional Endothelial Cells
Article Snippet: .. Extracted RNA was treated with DNase I (Ambion) in the presence of RNasin (Promega) to remove DNA contamination before cDNA synthesis. cDNA was synthesized with oligodeoxythymidylic acid primers (Boehringer Mannheim) and Superscript 11 reverse transcriptase (Life Technologies, Inc.). .. The cDNA primers and PCR conditions for the amplification of the endothelial cell markers are described in this supplement.

Article Title: Rheumatoid Arthritis Fibroblast-like Synoviocyte Suppression Mediated by PTEN Involves Survivin Gene Silencing
Article Snippet: .. First strand cDNA was synthesized by oligo(dT) primers and Moloney murine leukemia virus (M-MuLV) reverse transcriptase (Boehringer-Mannheim, La Jolla, California, USA). .. Plasmid construction A 1212 base pair PTEN cDNA fragment was amplified using polymerase chain reaction (PCR) with primers of fwd: 5′-ATAGGATCCATGACAGCCATCATCAAAGAG-3′ and rev: 5′-CCGCTCGAGTTTCATGGTGTTTTATCCCTC-3′ with KOD-Plus-DNA-polymerase (Toyobo Co., Ltd., Japan) from cDNA of peripheral blood mononuclear cells, digested with BamH /Xho I and cloned into pcDNA3.1 vector.

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  • 92
    Boehringer Mannheim oligo dt primer
    CDDO-Me inhibits hTERT expression in pancreatic cancer cells A. Effect of CDDO-Me on hTERT gene expression. Panc-1 and Mia PaCa-2 cells were treated with CDDO-Me (0–0.5 μM) for 5 days and total cellular <t>RNA</t> was prepared using TRI-zole reagent. 1 μg of cellular RNA was reverse transcribed using <t>oligo-dt</t> primer and high fidelity reverse transcriptase. 1 μL of cDNA was amplified using hTERT or GAPDH primers. Amplified products were separated on 2% DNA agarose gel. Gels were stained with ethidium bromide and DNA fragments were identified by base pair size. B. Effect of CDDO-Me on hTERT protein. Panc-1 and MiaPaCa-2 cells were treated with CDDO-Me as described above and cell lysates were analyzed for hTERT and p-hTERT protein by western blotting. Each experiment was repeated at least three times.
    Oligo Dt Primer, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo dt primer/product/Boehringer Mannheim
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    oligo dt primer - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Boehringer Mannheim oligo dt columns
    (A) Primer extension of bovine kidney outer medullar RNA (20 μg) (lane 1) and tRNA (lane 2) with primers, bTHP4 and bTHP7 (not shown), randomly chosen from exon 2. The major extension product identified is 16 nucleotides upstream from the 5′ end of bTHP1. A sequencing ladder was produced with bTHP1 and the same <t>oligo</t> primers. (B) Primer extension analysis of rat kidney mRNA (2 μg) with primers <t>RTHPRE36</t> (lane 1) and RTH5 (lane 2). Lanes 3 and 4 are extensions using rat liver mRNA (2 μg) and primers RTHPRE36 and RTH5, respectively. The major extension products (102 nt in lane 1 and 159 nt in lane 2) are marked by arrows. A sequencing ladder was run next to the reactions for sizing purposes.
    Oligo Dt Columns, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo dt columns/product/Boehringer Mannheim
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    oligo dt columns - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Boehringer Mannheim oligo
    (A) Primer extension of bovine kidney outer medullar RNA (20 μg) (lane 1) and tRNA (lane 2) with primers, bTHP4 and bTHP7 (not shown), randomly chosen from exon 2. The major extension product identified is 16 nucleotides upstream from the 5′ end of bTHP1. A sequencing ladder was produced with bTHP1 and the same <t>oligo</t> primers. (B) Primer extension analysis of rat kidney mRNA (2 μg) with primers <t>RTHPRE36</t> (lane 1) and RTH5 (lane 2). Lanes 3 and 4 are extensions using rat liver mRNA (2 μg) and primers RTHPRE36 and RTH5, respectively. The major extension products (102 nt in lane 1 and 159 nt in lane 2) are marked by arrows. A sequencing ladder was run next to the reactions for sizing purposes.
    Oligo, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo/product/Boehringer Mannheim
    Average 92 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    oligo - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    CDDO-Me inhibits hTERT expression in pancreatic cancer cells A. Effect of CDDO-Me on hTERT gene expression. Panc-1 and Mia PaCa-2 cells were treated with CDDO-Me (0–0.5 μM) for 5 days and total cellular RNA was prepared using TRI-zole reagent. 1 μg of cellular RNA was reverse transcribed using oligo-dt primer and high fidelity reverse transcriptase. 1 μL of cDNA was amplified using hTERT or GAPDH primers. Amplified products were separated on 2% DNA agarose gel. Gels were stained with ethidium bromide and DNA fragments were identified by base pair size. B. Effect of CDDO-Me on hTERT protein. Panc-1 and MiaPaCa-2 cells were treated with CDDO-Me as described above and cell lysates were analyzed for hTERT and p-hTERT protein by western blotting. Each experiment was repeated at least three times.

    Journal: Journal of carcinogenesis & mutagenesis

    Article Title: Induction of Apoptosis in Pancreatic Cancer Cells by CDDO-Me Involves Repression of Telomerase through Epigenetic Pathways

    doi: 10.4172/2157-2518.1000177

    Figure Lengend Snippet: CDDO-Me inhibits hTERT expression in pancreatic cancer cells A. Effect of CDDO-Me on hTERT gene expression. Panc-1 and Mia PaCa-2 cells were treated with CDDO-Me (0–0.5 μM) for 5 days and total cellular RNA was prepared using TRI-zole reagent. 1 μg of cellular RNA was reverse transcribed using oligo-dt primer and high fidelity reverse transcriptase. 1 μL of cDNA was amplified using hTERT or GAPDH primers. Amplified products were separated on 2% DNA agarose gel. Gels were stained with ethidium bromide and DNA fragments were identified by base pair size. B. Effect of CDDO-Me on hTERT protein. Panc-1 and MiaPaCa-2 cells were treated with CDDO-Me as described above and cell lysates were analyzed for hTERT and p-hTERT protein by western blotting. Each experiment was repeated at least three times.

    Article Snippet: 1 μg of RNA was then reverse transcribed by oligo-dt primer and high fidelity reverse transcriptase (Boehringer Mannheim, Germany) to generate cDNAs.

    Techniques: Expressing, Amplification, Agarose Gel Electrophoresis, Staining, Western Blot

    (A) Primer extension of bovine kidney outer medullar RNA (20 μg) (lane 1) and tRNA (lane 2) with primers, bTHP4 and bTHP7 (not shown), randomly chosen from exon 2. The major extension product identified is 16 nucleotides upstream from the 5′ end of bTHP1. A sequencing ladder was produced with bTHP1 and the same oligo primers. (B) Primer extension analysis of rat kidney mRNA (2 μg) with primers RTHPRE36 (lane 1) and RTH5 (lane 2). Lanes 3 and 4 are extensions using rat liver mRNA (2 μg) and primers RTHPRE36 and RTH5, respectively. The major extension products (102 nt in lane 1 and 159 nt in lane 2) are marked by arrows. A sequencing ladder was run next to the reactions for sizing purposes.

    Journal: Gene Expression

    Article Title: Bovine and Rodent Tamm-Horsfall Protein (THP) Genes: Cloning, Structural Analysis, and Promoter Identification

    doi:

    Figure Lengend Snippet: (A) Primer extension of bovine kidney outer medullar RNA (20 μg) (lane 1) and tRNA (lane 2) with primers, bTHP4 and bTHP7 (not shown), randomly chosen from exon 2. The major extension product identified is 16 nucleotides upstream from the 5′ end of bTHP1. A sequencing ladder was produced with bTHP1 and the same oligo primers. (B) Primer extension analysis of rat kidney mRNA (2 μg) with primers RTHPRE36 (lane 1) and RTH5 (lane 2). Lanes 3 and 4 are extensions using rat liver mRNA (2 μg) and primers RTHPRE36 and RTH5, respectively. The major extension products (102 nt in lane 1 and 159 nt in lane 2) are marked by arrows. A sequencing ladder was run next to the reactions for sizing purposes.

    Article Snippet: Primer extension analysis was carried out as described by R. Kingston ( ) with specific exon 2 oligos BTHP4 and BTHP7 for bovine samples and RTHPRE36 and RTH5 for rat samples. mRNA was prepared by using oligo-dT columns (Boehringer Mannheim).

    Techniques: Sequencing, Produced