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TaKaRa oligodeoxythymidylic acid primer system
Oligodeoxythymidylic Acid Primer System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Down-regulation of tumor suppressor gene FEZ1/LZTS1 in breast carcinoma involves promoter methylation and associates with metastasis
Article Snippet: .. The first strand cDNA was synthesized from total RNA using the Iscript cDNA synthesis kit (Bio-Rad Laboratories, CA, USA) or the oligodeoxythymidylic acid primer system (TaKaRa, Japan). .. An RNA sample from a pool of normal human breast tissues (Clontech, Palo Alto, CA) was used as the normal control.

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  • 85
    TaKaRa adapter linked oligo dt primer
    RT–PCR analysis. ( A ) The DHFR gene structure is diagramed. Black boxes indicate exons. Primer sets used for PCR are shown. ( B ) Poly(A) + RNA from control mock-transfected (lane 1) or from siRNA-408 and siRNA-613 transfected CFIm25 knocked-down (lanes 2 and 3) <t>HeLa</t> cells were reverse transcribed using an <t>oligo-dT</t> primer, and then DHFR cDNA fragments were amplified by PCR. A 100 bp DNA ladder was loaded in lane M.
    Adapter Linked Oligo Dt Primer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adapter linked oligo dt primer/product/TaKaRa
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    adapter linked oligo dt primer - by Bioz Stars, 2020-08
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    94
    TaKaRa oligo dt primers
    Definition of messenger RNAs transcribed from MSBI1.176. ( a ) Linear representation of the MSBI1.176 genome including ORFs larger than 40 amino acids for the sense strand. The position of the <t>RNA</t> probe for northern blot analyses (Fig. 2e) is indicated. For 5′-RACE, primers located in different regions of the Rep-ORF were used (indicated as red, yellow and blue arrows). Each reproducibly detected transcription start site for each primer is plotted against the location within the genome. For 3′-RACE, nested PCRs were performed with the indicated primers (black and grey arrows). Each reproducibly detected polyadenylation site is plotted against the location within the genome. Putative transcripts arising from the combination of the 5′- and 3′-RACE results, whose existence was supported by northern blot, and their coding potentials are shown. ( b ) Agarose gel image of the MSBI1.176-specific 5′-RACE products obtained by PCR using the primers indicated in panel (a). cDNA of mock-transfected cells was used as a negative control. Specific start sites are indicated by letters according to panel ( a ). ( c ) Agarose gel image of the respective 3′-RACE products obtained by nested PCR using the primers indicated in panel (a). Specific polyadenylation sites are indicated as letters according to panel (a). ( d ) Validation of continuous transcripts using RT-PCR. Total RNA of MSBI1.176-transfected cells was reverse transcribed using <t>oligo-dT</t> primers (+RT) and the resulting cDNA was subjected to PCR reactions using the indicated primer combinations. RT reactions lacking reverse transcriptase served as negative controls (−RT) and PCR reactions using linearized MSBI1.176 DNA as a template served as a positive control.( e ) Northern blot validation of MSBI1.176 transcripts in HEK293TT cells using an antisense RNA probe as indicated in ( a ). Mock-transfected cells served as a negative control circular MSBI1.176 genome served as a positive control. ( f ) Detection of MSBI1.176 Rep translation in HEK293TT cells transfected with a genome version containing a N-terminal FLAG tag fused to the Rep ORF (F-Rep) using an anti-FLAG antibody. An overexpressed Rep-FLAG fusion protein was used as a positive control and total protein of mock-transfected cells was used as a negative control. Gamma tubulin was used as a loading control.
    Oligo Dt Primers, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 420 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo dt primers/product/TaKaRa
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    88
    TaKaRa advantage reverse transcriptase for pcr kit
    P2X2 channels are the targets for ATP at interneuron synapses. A , Top left, Diagrammatic representation of the endogenous mouse P2X2 (mP2X2) locus with 11 exons. Top right, Representation of the targeted locus with exons 2-11 deleted and replaced with Neo. In both diagrams, the black bars indicate the approximate positions of the primers used for <t>PCR</t> analysis of tail genomic DNA, as shown in the gel to the left. Both the PCR and Southern blot (results not shown) approaches show that the mP2X2 gene is disrupted with Neo, but because we ran the PCR screen with all three primers, this approach additionally shows that exons downstream of exon 1 are deleted because no wild-type band was detected (left gel). Right gel, RT-PCR results from P2X2 + / + and P2X2 - / - mice with <t>mRNA</t> harvested from brain and testis. There was P2X2 mRNA in both brain and testis from P2X2 + / + mice but not from P2X2 - / - mice. mRNA for β-actin (β-act) was present in both genotypes and tissues. mw, Molecular weight markers. B, C , Nissl staining in P2X2 + / + ( B ) and P2X2 - / - mice ( C ). The representative voltage waveforms show responses of interneurons to depolarizing and hyperpolarizing current injections. D , EPSC frequency against time for 11 interneurons recorded from a P2X2 + / + mouse; the inset indicates that 64% of neurons responded to P2X activation in this mouse. E , mEPSC frequency against time for 13 interneurons recorded from a P2X2 - / - mouse; the inset indicates that only 15% of neurons responded to P2X activation in this mouse. F , Summary of recordings for all neurons that responded to ATPγS from seven P2X2 - / - and seven P2X2 + / + mice. G , In the P2X2 - / - mice, the proportion of neurons that receive P2X-modulated synapses is significantly reduced ( p
    Advantage Reverse Transcriptase For Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/advantage reverse transcriptase for pcr kit/product/TaKaRa
    Average 88 stars, based on 9 article reviews
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    RT–PCR analysis. ( A ) The DHFR gene structure is diagramed. Black boxes indicate exons. Primer sets used for PCR are shown. ( B ) Poly(A) + RNA from control mock-transfected (lane 1) or from siRNA-408 and siRNA-613 transfected CFIm25 knocked-down (lanes 2 and 3) HeLa cells were reverse transcribed using an oligo-dT primer, and then DHFR cDNA fragments were amplified by PCR. A 100 bp DNA ladder was loaded in lane M.

    Journal: Nucleic Acids Research

    Article Title: Knock-down of 25 kDa subunit of cleavage factor Im in Hela cells alters alternative polyadenylation within 3?-UTRs

    doi: 10.1093/nar/gkl794

    Figure Lengend Snippet: RT–PCR analysis. ( A ) The DHFR gene structure is diagramed. Black boxes indicate exons. Primer sets used for PCR are shown. ( B ) Poly(A) + RNA from control mock-transfected (lane 1) or from siRNA-408 and siRNA-613 transfected CFIm25 knocked-down (lanes 2 and 3) HeLa cells were reverse transcribed using an oligo-dT primer, and then DHFR cDNA fragments were amplified by PCR. A 100 bp DNA ladder was loaded in lane M.

    Article Snippet: 3′ RACE analysis and RT–PCR analysis For 3′ RACE experiments, 1 μg of total RNA, from control HeLa cells or knocked-down cells, was reverse transcribed with an adapter linked oligo-dT primer to the final volume of 20 μl following the manufacture's procedure (3′-Full RACE Core Set, TAKARA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Transfection, Amplification

    Definition of messenger RNAs transcribed from MSBI1.176. ( a ) Linear representation of the MSBI1.176 genome including ORFs larger than 40 amino acids for the sense strand. The position of the RNA probe for northern blot analyses (Fig. 2e) is indicated. For 5′-RACE, primers located in different regions of the Rep-ORF were used (indicated as red, yellow and blue arrows). Each reproducibly detected transcription start site for each primer is plotted against the location within the genome. For 3′-RACE, nested PCRs were performed with the indicated primers (black and grey arrows). Each reproducibly detected polyadenylation site is plotted against the location within the genome. Putative transcripts arising from the combination of the 5′- and 3′-RACE results, whose existence was supported by northern blot, and their coding potentials are shown. ( b ) Agarose gel image of the MSBI1.176-specific 5′-RACE products obtained by PCR using the primers indicated in panel (a). cDNA of mock-transfected cells was used as a negative control. Specific start sites are indicated by letters according to panel ( a ). ( c ) Agarose gel image of the respective 3′-RACE products obtained by nested PCR using the primers indicated in panel (a). Specific polyadenylation sites are indicated as letters according to panel (a). ( d ) Validation of continuous transcripts using RT-PCR. Total RNA of MSBI1.176-transfected cells was reverse transcribed using oligo-dT primers (+RT) and the resulting cDNA was subjected to PCR reactions using the indicated primer combinations. RT reactions lacking reverse transcriptase served as negative controls (−RT) and PCR reactions using linearized MSBI1.176 DNA as a template served as a positive control.( e ) Northern blot validation of MSBI1.176 transcripts in HEK293TT cells using an antisense RNA probe as indicated in ( a ). Mock-transfected cells served as a negative control circular MSBI1.176 genome served as a positive control. ( f ) Detection of MSBI1.176 Rep translation in HEK293TT cells transfected with a genome version containing a N-terminal FLAG tag fused to the Rep ORF (F-Rep) using an anti-FLAG antibody. An overexpressed Rep-FLAG fusion protein was used as a positive control and total protein of mock-transfected cells was used as a negative control. Gamma tubulin was used as a loading control.

    Journal: Scientific Reports

    Article Title: Expression and replication of virus-like circular DNA in human cells

    doi: 10.1038/s41598-018-21317-w

    Figure Lengend Snippet: Definition of messenger RNAs transcribed from MSBI1.176. ( a ) Linear representation of the MSBI1.176 genome including ORFs larger than 40 amino acids for the sense strand. The position of the RNA probe for northern blot analyses (Fig. 2e) is indicated. For 5′-RACE, primers located in different regions of the Rep-ORF were used (indicated as red, yellow and blue arrows). Each reproducibly detected transcription start site for each primer is plotted against the location within the genome. For 3′-RACE, nested PCRs were performed with the indicated primers (black and grey arrows). Each reproducibly detected polyadenylation site is plotted against the location within the genome. Putative transcripts arising from the combination of the 5′- and 3′-RACE results, whose existence was supported by northern blot, and their coding potentials are shown. ( b ) Agarose gel image of the MSBI1.176-specific 5′-RACE products obtained by PCR using the primers indicated in panel (a). cDNA of mock-transfected cells was used as a negative control. Specific start sites are indicated by letters according to panel ( a ). ( c ) Agarose gel image of the respective 3′-RACE products obtained by nested PCR using the primers indicated in panel (a). Specific polyadenylation sites are indicated as letters according to panel (a). ( d ) Validation of continuous transcripts using RT-PCR. Total RNA of MSBI1.176-transfected cells was reverse transcribed using oligo-dT primers (+RT) and the resulting cDNA was subjected to PCR reactions using the indicated primer combinations. RT reactions lacking reverse transcriptase served as negative controls (−RT) and PCR reactions using linearized MSBI1.176 DNA as a template served as a positive control.( e ) Northern blot validation of MSBI1.176 transcripts in HEK293TT cells using an antisense RNA probe as indicated in ( a ). Mock-transfected cells served as a negative control circular MSBI1.176 genome served as a positive control. ( f ) Detection of MSBI1.176 Rep translation in HEK293TT cells transfected with a genome version containing a N-terminal FLAG tag fused to the Rep ORF (F-Rep) using an anti-FLAG antibody. An overexpressed Rep-FLAG fusion protein was used as a positive control and total protein of mock-transfected cells was used as a negative control. Gamma tubulin was used as a loading control.

    Article Snippet: Reverse transcription (RT) PCR and long PCR For cDNA synthesis, 1 µg of DNase1-treated total RNA was reverse transcribed using random hexamer or oligo-dT primers using SMARTScribe Reverse Transcriptase (Clontech) as recommended by the manufacturer.

    Techniques: Northern Blot, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Transfection, Negative Control, Nested PCR, Reverse Transcription Polymerase Chain Reaction, Positive Control, FLAG-tag

    Definition of messenger RNAs transcribed from CMI1.252. ( a ) Linear representation of the CMI1.252 genome including ORFs larger than 40 amino acids for the sense strand. The position of the RNA probes for northern blot analyses (Fig. 3e) are indicated. For 5′-RACE, nested PCR was performed using primers located within the Rep-ORF (indicated as red arrows) and within the ORF-2 (blue arrows). Each reproducibly detected transcription start site for each primer is plotted against the location within the genome. For 3′-RACE, nested PCR was performed using the indicated primers (black and grey arrows). Each reproducibly detected polyadenylation site is plotted against the location within the genome. Transcripts arising from the combination of the 5′- and 3′-RACE results, whose existence was supported by northern blots, as well as their coding potentials are shown. ( b ) Agarose gel image of the CMI1.252-specific 5′-RACE products obtained by PCR using the different primers indicated in panel (a). cDNA of mock-transfected cells was used as a negative control. Specific start sites are indicated by letters according to panel (a). ( c ) Agarose gel image of the respective 3′-RACE products obtained by nested PCR using the primers indicated in panel (a). Specific polyadenylation sites are indicated as letters according to panel (a). ( d ) Validation of continuous transcripts using RT-PCR. Total RNA of CMI1.252-transfected cells was reverse transcribed using oligo-dT primers (+RT) and the resulting cDNA was subjected to PCR reactions using the indicated primer combinations. RT reactions lacking reverse transcriptase was used as negative controls (−RT) and PCR reactions using linearized CMI1.252 DNA as a template served as a positive control. ( e ) Northern blot validation of CMI1.252 transcripts in HEK293TT cells. Antisense RNA probes complementary to nt 883–1051 (probe 1) or nt 2074–2264 (probe 2) of CMI1.252 were used to detect transcripts in CMI1.252-transfected HEK293TT cells. Mock-transfected cells served as a negative control and circular CMI1.252 genome served as a positive control.

    Journal: Scientific Reports

    Article Title: Expression and replication of virus-like circular DNA in human cells

    doi: 10.1038/s41598-018-21317-w

    Figure Lengend Snippet: Definition of messenger RNAs transcribed from CMI1.252. ( a ) Linear representation of the CMI1.252 genome including ORFs larger than 40 amino acids for the sense strand. The position of the RNA probes for northern blot analyses (Fig. 3e) are indicated. For 5′-RACE, nested PCR was performed using primers located within the Rep-ORF (indicated as red arrows) and within the ORF-2 (blue arrows). Each reproducibly detected transcription start site for each primer is plotted against the location within the genome. For 3′-RACE, nested PCR was performed using the indicated primers (black and grey arrows). Each reproducibly detected polyadenylation site is plotted against the location within the genome. Transcripts arising from the combination of the 5′- and 3′-RACE results, whose existence was supported by northern blots, as well as their coding potentials are shown. ( b ) Agarose gel image of the CMI1.252-specific 5′-RACE products obtained by PCR using the different primers indicated in panel (a). cDNA of mock-transfected cells was used as a negative control. Specific start sites are indicated by letters according to panel (a). ( c ) Agarose gel image of the respective 3′-RACE products obtained by nested PCR using the primers indicated in panel (a). Specific polyadenylation sites are indicated as letters according to panel (a). ( d ) Validation of continuous transcripts using RT-PCR. Total RNA of CMI1.252-transfected cells was reverse transcribed using oligo-dT primers (+RT) and the resulting cDNA was subjected to PCR reactions using the indicated primer combinations. RT reactions lacking reverse transcriptase was used as negative controls (−RT) and PCR reactions using linearized CMI1.252 DNA as a template served as a positive control. ( e ) Northern blot validation of CMI1.252 transcripts in HEK293TT cells. Antisense RNA probes complementary to nt 883–1051 (probe 1) or nt 2074–2264 (probe 2) of CMI1.252 were used to detect transcripts in CMI1.252-transfected HEK293TT cells. Mock-transfected cells served as a negative control and circular CMI1.252 genome served as a positive control.

    Article Snippet: Reverse transcription (RT) PCR and long PCR For cDNA synthesis, 1 µg of DNase1-treated total RNA was reverse transcribed using random hexamer or oligo-dT primers using SMARTScribe Reverse Transcriptase (Clontech) as recommended by the manufacturer.

    Techniques: Northern Blot, Nested PCR, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Transfection, Negative Control, Reverse Transcription Polymerase Chain Reaction, Positive Control

    Identification and characterization of LOC100133669. A, Schematic diagram of LOC100133669 from NCBI RefSeq and RACE. Blue boxes, exons annotated by NCBI RefSeq. Orange boxes, additional sequence and exon determined by RACE. Blue lines, introns. Arrows on blue lines indicate transcriptional directions. B, Gel electrophoresis of nested PCR products from 5′‐RACE and 3′‐RACE. Arrows indicate purpose strips. C, The full‐length sequence of LOC100133669 transcript. Blue, reference sequence from NCBI RefSeq. Orange, added sequence from RACE. D, E, ORF finder software (D) and CPAT software (E) prediction for protein‐coding potential of LOC100133669. F, Gel electrophoresis of PCR products reversed with oligo dT primer or random 6 mers, respectively, in KYSE150, KYSE410, KYSE450 and KYSE510 cells. G, Subcellular distribution of LOC100133669 in KYSE450 and KYSE510 cells. GAPDH and myc precursor RNA (pre‐myc) were served as cytoplasmic and nuclear references, respectively. Data are presented as mean ± SD, n = 3

    Journal: Cell Proliferation

    Article Title: Long non‐coding RNA LOC100133669 promotes cell proliferation in oesophageal squamous cell carcinoma, et al. Long non‐coding RNA LOC100133669 promotes cell proliferation in oesophageal squamous cell carcinoma

    doi: 10.1111/cpr.12750

    Figure Lengend Snippet: Identification and characterization of LOC100133669. A, Schematic diagram of LOC100133669 from NCBI RefSeq and RACE. Blue boxes, exons annotated by NCBI RefSeq. Orange boxes, additional sequence and exon determined by RACE. Blue lines, introns. Arrows on blue lines indicate transcriptional directions. B, Gel electrophoresis of nested PCR products from 5′‐RACE and 3′‐RACE. Arrows indicate purpose strips. C, The full‐length sequence of LOC100133669 transcript. Blue, reference sequence from NCBI RefSeq. Orange, added sequence from RACE. D, E, ORF finder software (D) and CPAT software (E) prediction for protein‐coding potential of LOC100133669. F, Gel electrophoresis of PCR products reversed with oligo dT primer or random 6 mers, respectively, in KYSE150, KYSE410, KYSE450 and KYSE510 cells. G, Subcellular distribution of LOC100133669 in KYSE450 and KYSE510 cells. GAPDH and myc precursor RNA (pre‐myc) were served as cytoplasmic and nuclear references, respectively. Data are presented as mean ± SD, n = 3

    Article Snippet: 2.6 RNA extraction, reverse transcription‐quantitative PCR (RT‐qPCR) and semi‐quantitative PCR Total RNA extracted from ESCC cells with TRIzol reagent (Invitrogen) was reverse‐transcribed to cDNA with oligo dT primer or random 6 mers using a PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara, China). qPCR was performed using SYBR Premix Ex Taq (Takara, China) on a Stratagene Mx3000P qPCR System (Agilent Technologies).

    Techniques: Sequencing, Nucleic Acid Electrophoresis, Nested PCR, Software, Polymerase Chain Reaction

    P2X2 channels are the targets for ATP at interneuron synapses. A , Top left, Diagrammatic representation of the endogenous mouse P2X2 (mP2X2) locus with 11 exons. Top right, Representation of the targeted locus with exons 2-11 deleted and replaced with Neo. In both diagrams, the black bars indicate the approximate positions of the primers used for PCR analysis of tail genomic DNA, as shown in the gel to the left. Both the PCR and Southern blot (results not shown) approaches show that the mP2X2 gene is disrupted with Neo, but because we ran the PCR screen with all three primers, this approach additionally shows that exons downstream of exon 1 are deleted because no wild-type band was detected (left gel). Right gel, RT-PCR results from P2X2 + / + and P2X2 - / - mice with mRNA harvested from brain and testis. There was P2X2 mRNA in both brain and testis from P2X2 + / + mice but not from P2X2 - / - mice. mRNA for β-actin (β-act) was present in both genotypes and tissues. mw, Molecular weight markers. B, C , Nissl staining in P2X2 + / + ( B ) and P2X2 - / - mice ( C ). The representative voltage waveforms show responses of interneurons to depolarizing and hyperpolarizing current injections. D , EPSC frequency against time for 11 interneurons recorded from a P2X2 + / + mouse; the inset indicates that 64% of neurons responded to P2X activation in this mouse. E , mEPSC frequency against time for 13 interneurons recorded from a P2X2 - / - mouse; the inset indicates that only 15% of neurons responded to P2X activation in this mouse. F , Summary of recordings for all neurons that responded to ATPγS from seven P2X2 - / - and seven P2X2 + / + mice. G , In the P2X2 - / - mice, the proportion of neurons that receive P2X-modulated synapses is significantly reduced ( p

    Journal: The Journal of Neuroscience

    Article Title: ATP Modulation of Excitatory Synapses onto Interneurons

    doi: 10.1523/JNEUROSCI.23-19-07426.2003

    Figure Lengend Snippet: P2X2 channels are the targets for ATP at interneuron synapses. A , Top left, Diagrammatic representation of the endogenous mouse P2X2 (mP2X2) locus with 11 exons. Top right, Representation of the targeted locus with exons 2-11 deleted and replaced with Neo. In both diagrams, the black bars indicate the approximate positions of the primers used for PCR analysis of tail genomic DNA, as shown in the gel to the left. Both the PCR and Southern blot (results not shown) approaches show that the mP2X2 gene is disrupted with Neo, but because we ran the PCR screen with all three primers, this approach additionally shows that exons downstream of exon 1 are deleted because no wild-type band was detected (left gel). Right gel, RT-PCR results from P2X2 + / + and P2X2 - / - mice with mRNA harvested from brain and testis. There was P2X2 mRNA in both brain and testis from P2X2 + / + mice but not from P2X2 - / - mice. mRNA for β-actin (β-act) was present in both genotypes and tissues. mw, Molecular weight markers. B, C , Nissl staining in P2X2 + / + ( B ) and P2X2 - / - mice ( C ). The representative voltage waveforms show responses of interneurons to depolarizing and hyperpolarizing current injections. D , EPSC frequency against time for 11 interneurons recorded from a P2X2 + / + mouse; the inset indicates that 64% of neurons responded to P2X activation in this mouse. E , mEPSC frequency against time for 13 interneurons recorded from a P2X2 - / - mouse; the inset indicates that only 15% of neurons responded to P2X activation in this mouse. F , Summary of recordings for all neurons that responded to ATPγS from seven P2X2 - / - and seven P2X2 + / + mice. G , In the P2X2 - / - mice, the proportion of neurons that receive P2X-modulated synapses is significantly reduced ( p

    Article Snippet: We isolated mRNA from whole brain and testis with oligo-dT beads using the Quick Prep Micro mRNA purification kit (Amersham Biosciences, Arlington Heights, IL). cDNA was reverse-transcribed from mRNA using Moloney murine leukemia virus reverse transcriptase and oligo-dT primers (Advantage reverse transcriptase for PCR kit; Clontech, Palo Alto, CA), and PCR was performed using Taq polymerase ( Taq PCR core kit; Qiagen, Hilden, Germany).

    Techniques: Polymerase Chain Reaction, Southern Blot, Reverse Transcription Polymerase Chain Reaction, Mouse Assay, Activated Clotting Time Assay, Molecular Weight, Staining, Activation Assay