Structured Review

Thermo Fisher oligo
Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1893 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oligo/product/Thermo Fisher
Average 99 stars, based on 1893 article reviews
Price from $9.99 to $1999.99
oligo - by Bioz Stars, 2020-01
99/100 stars

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Amplification:

Article Title: Hepatocyte Growth Factor Regulates Migration of Olfactory Interneuron Precursors in the Rostral Migratory Stream through Met–Grb2 Coupling
Article Snippet: Five hundred nanograms of total RNA were reverse transcribed into single stranded cDNA in a volume of 20 μl using 200 U of M-MLV Reverse-Transcriptase RNaseH (Invitrogen) in 1× buffer (Invitrogen) with 25 μg/ml oligo(dT)12–18 (Invitrogen), 0.5 m m dNTPs (Invitrogen), 0.01 m DTT(Invitrogen), and 2 U/μl RNaseOUT (Invitrogen). .. The following primers were used: 5′-GGGACTGCAGCAGCAAAGC-3′ and 5′-GTCTGAGCATCTAGAGTTTCC-3′ for Met amplification, corresponding to nucleotides 296–815 ( ); 5′-GGGGAATGAGAAATGCAGTCAG-3′ and 5′-CCTGTATCCATGGATGCTTC-3′ for HGF amplification, corresponding to nucleotides 1981–2275 ( ); and 5′-CCTGAAGGTCAAAGGGAATGTGTTC-3′ and 5′-GGACAGAGTCTTGATGATCTCCTCC-3′ for L-19 amplification, corresponding to nucleotides 400–595 (gi56971151).

Isolation:

Article Title: Hepatocyte Growth Factor Regulates Migration of Olfactory Interneuron Precursors in the Rostral Migratory Stream through Met–Grb2 Coupling
Article Snippet: Total RNA was isolated from aRMS, posterior RMS (pRMS), SVZ, and total OB of P5 mice using Qiagen columns according to the manufacturer's instructions. .. Five hundred nanograms of total RNA were reverse transcribed into single stranded cDNA in a volume of 20 μl using 200 U of M-MLV Reverse-Transcriptase RNaseH (Invitrogen) in 1× buffer (Invitrogen) with 25 μg/ml oligo(dT)12–18 (Invitrogen), 0.5 m m dNTPs (Invitrogen), 0.01 m DTT(Invitrogen), and 2 U/μl RNaseOUT (Invitrogen).

Size-exclusion Chromatography:

Article Title: Involvement of Brain-Derived Neurotrophic Factor in Spatial Memory Formation and Maintenance in a Radial Arm Maze Test in Rats
Article Snippet: The time taken to consume all 10 baits was recorded, with a cutoff time of 180 sec ( ). .. Total RNA (1 μg) was converted into cDNA using oligo(dT) 12–18 primer (Life Technologies, Tokyo, Japan) and Moloney murine leukemia virus reverse transcriptase (Life Technologies) in a total reaction volume of 25 μl (RT-reaction mixture).

Quantitative RT-PCR:

Article Title: A Crucial Role for Primary Cilia in Cortical Morphogenesis
Article Snippet: Paragraph title: Quantitative real time RT-PCR ... 1–5 μg of RNA was transcribed into cDNA using oligo(dT)12–18 (0,5 μg/μl, Invitrogen) or random hexamers (50 m m , Applied Biosystems) and SuperScript II RNase H− reverse transcriptase (Invitrogen).

Mouse Assay:

Article Title: Hepatocyte Growth Factor Regulates Migration of Olfactory Interneuron Precursors in the Rostral Migratory Stream through Met–Grb2 Coupling
Article Snippet: Total RNA was isolated from aRMS, posterior RMS (pRMS), SVZ, and total OB of P5 mice using Qiagen columns according to the manufacturer's instructions. .. Five hundred nanograms of total RNA were reverse transcribed into single stranded cDNA in a volume of 20 μl using 200 U of M-MLV Reverse-Transcriptase RNaseH (Invitrogen) in 1× buffer (Invitrogen) with 25 μg/ml oligo(dT)12–18 (Invitrogen), 0.5 m m dNTPs (Invitrogen), 0.01 m DTT(Invitrogen), and 2 U/μl RNaseOUT (Invitrogen).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Involvement of Brain-Derived Neurotrophic Factor in Spatial Memory Formation and Maintenance in a Radial Arm Maze Test in Rats
Article Snippet: The levels of BDNF mRNA in brain tissues were determined by RT-PCR. .. Total RNA (1 μg) was converted into cDNA using oligo(dT) 12–18 primer (Life Technologies, Tokyo, Japan) and Moloney murine leukemia virus reverse transcriptase (Life Technologies) in a total reaction volume of 25 μl (RT-reaction mixture).

Article Title: Hepatocyte Growth Factor Regulates Migration of Olfactory Interneuron Precursors in the Rostral Migratory Stream through Met–Grb2 Coupling
Article Snippet: Expression of HGF, Met, and L-19 was analyzed by reverse transcription (RT)-PCR. .. Five hundred nanograms of total RNA were reverse transcribed into single stranded cDNA in a volume of 20 μl using 200 U of M-MLV Reverse-Transcriptase RNaseH (Invitrogen) in 1× buffer (Invitrogen) with 25 μg/ml oligo(dT)12–18 (Invitrogen), 0.5 m m dNTPs (Invitrogen), 0.01 m DTT(Invitrogen), and 2 U/μl RNaseOUT (Invitrogen).

Expressing:

Article Title: A Crucial Role for Primary Cilia in Cortical Morphogenesis
Article Snippet: 1–5 μg of RNA was transcribed into cDNA using oligo(dT)12–18 (0,5 μg/μl, Invitrogen) or random hexamers (50 m m , Applied Biosystems) and SuperScript II RNase H− reverse transcriptase (Invitrogen). .. Quantitative real time PCRs were performed using the ABI Prism 7000 Detection System (Applied Biosystems) using TaqMan Gene Expression Assays (Applied Biosystems) with 1 μl of cDNA (20 μl of RTase reactions using 1–5 μg of whole RNA input).

Article Title: Hepatocyte Growth Factor Regulates Migration of Olfactory Interneuron Precursors in the Rostral Migratory Stream through Met–Grb2 Coupling
Article Snippet: Expression of HGF, Met, and L-19 was analyzed by reverse transcription (RT)-PCR. .. Five hundred nanograms of total RNA were reverse transcribed into single stranded cDNA in a volume of 20 μl using 200 U of M-MLV Reverse-Transcriptase RNaseH (Invitrogen) in 1× buffer (Invitrogen) with 25 μg/ml oligo(dT)12–18 (Invitrogen), 0.5 m m dNTPs (Invitrogen), 0.01 m DTT(Invitrogen), and 2 U/μl RNaseOUT (Invitrogen).

Polymerase Chain Reaction:

Article Title: Involvement of Brain-Derived Neurotrophic Factor in Spatial Memory Formation and Maintenance in a Radial Arm Maze Test in Rats
Article Snippet: Total RNA (1 μg) was converted into cDNA using oligo(dT) 12–18 primer (Life Technologies, Tokyo, Japan) and Moloney murine leukemia virus reverse transcriptase (Life Technologies) in a total reaction volume of 25 μl (RT-reaction mixture). .. PCR was performed using one twenty-fifth of the RT-reaction mixture, 0.5 μ m of each (forward and reverse) primers, and ReadyToGo PCR Beads (Amersham Pharmacia Biotech, Tokyo, Japan) in a total reaction volume of 25 μl.

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