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Bio-Rad oligo dt primers
Oligo Dt Primers, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oligo dt primers/product/Bio-Rad
Average 93 stars, based on 98 article reviews
Price from $9.99 to $1999.99
oligo dt primers - by Bioz Stars, 2020-11
93/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: Notch-Mediated Suppression of TSC2 Expression Regulates Cell Differentiation in the Drosophila Intestinal Stem Cell Lineage
Article Snippet: .. Reverse transcription and real-time PCR Total RNA was extracted using Trizol. cDNAs were synthesized using oligo-dT primers and real-time RTPCR was performed on a BioRad iQ5 detection system (using SYBR Green and ΔΔCt quantification method). .. Gigas and Suppressor of Hairless expression levels were quantified relative to Actin5c expression.

Article Title: Neural JNK3 regulates blood flow recovery after hindlimb ischemia in mice via an Egr1/Creb1 axis
Article Snippet: .. RNA preparation and quantitative polymerase chain reaction Total RNA was extracted from cells and tissues with the RNeasy Mini Kit (Qiagen) or TRIzol reagent (Invitrogen), and 1 μg of total RNA was reverse transcribed with oligo(dT) primers for cDNA synthesis using an iScript cDNA synthesis kit (Biorad). .. The expression of mRNA was examined by quantitative PCR analysis using a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems).

Article Title: RALFL34 regulates formative cell divisions in Arabidopsis pericycle during lateral root initiation
Article Snippet: .. Quantitative real-time PCR (qRT-PCR) Total RNA was extracted from lines of interest using an RNeasy kit (Qiagen) as per the manufacturer’s instructions. cDNA was generated from 1 µg or 200ng of total RNA via oligo(dT) primers using iScript (BioRad) or Superscript II (Invitrogen), respectively, as per the instructions. .. Quantitative PCRs were performed in at least two technical repeats, using the SYBR Green QPCR Master Mix (Quanta Biosciences), in our modified reaction protocol: Master mix, 7 μl per reaction [forward primer (100 μM), 0.1 μl per reaction; reverse primer (100 μM), 0.1 μl per reaction; SYBR (2×), 6 μl per reaction; dH2 O, 0.8 μl per reaction] and template cDNA (diluted 1/100 in dH2 O, 5 μl per reaction.

Synthesized:

Article Title: Notch-Mediated Suppression of TSC2 Expression Regulates Cell Differentiation in the Drosophila Intestinal Stem Cell Lineage
Article Snippet: .. Reverse transcription and real-time PCR Total RNA was extracted using Trizol. cDNAs were synthesized using oligo-dT primers and real-time RTPCR was performed on a BioRad iQ5 detection system (using SYBR Green and ΔΔCt quantification method). .. Gigas and Suppressor of Hairless expression levels were quantified relative to Actin5c expression.

Random Hexamer Labeling:

Article Title: TRESK two-pore-domain K+ channels constitute a significant component of background potassium currents in murine dorsal root ganglion neurones
Article Snippet: .. Total RNA (0.5 μg) of individual animals was reverse transcribed with random hexamer and oligo-dT primers using an iScript cDNA synthesis kit (Bio-Rad Laboratories, Hercules, CA, USA). ..

Quantitative RT-PCR:

Article Title: RALFL34 regulates formative cell divisions in Arabidopsis pericycle during lateral root initiation
Article Snippet: .. Quantitative real-time PCR (qRT-PCR) Total RNA was extracted from lines of interest using an RNeasy kit (Qiagen) as per the manufacturer’s instructions. cDNA was generated from 1 µg or 200ng of total RNA via oligo(dT) primers using iScript (BioRad) or Superscript II (Invitrogen), respectively, as per the instructions. .. Quantitative PCRs were performed in at least two technical repeats, using the SYBR Green QPCR Master Mix (Quanta Biosciences), in our modified reaction protocol: Master mix, 7 μl per reaction [forward primer (100 μM), 0.1 μl per reaction; reverse primer (100 μM), 0.1 μl per reaction; SYBR (2×), 6 μl per reaction; dH2 O, 0.8 μl per reaction] and template cDNA (diluted 1/100 in dH2 O, 5 μl per reaction.

SYBR Green Assay:

Article Title: Notch-Mediated Suppression of TSC2 Expression Regulates Cell Differentiation in the Drosophila Intestinal Stem Cell Lineage
Article Snippet: .. Reverse transcription and real-time PCR Total RNA was extracted using Trizol. cDNAs were synthesized using oligo-dT primers and real-time RTPCR was performed on a BioRad iQ5 detection system (using SYBR Green and ΔΔCt quantification method). .. Gigas and Suppressor of Hairless expression levels were quantified relative to Actin5c expression.

Incubation:

Article Title: Embryonic poly(A)-binding protein (EPAB) is required for oocyte maturation and female fertility in mice
Article Snippet: .. Reverse transcription was performed using the RETROscript kit (Ambion) in two steps: first, template RNA and oligo(dT) primers were incubated at 85 °C for 3 min to eliminate any secondary structures, and then the buffer and enzyme were added and the reaction was carried out at 42 °C for 1 h. qRT-PCRs were carried out on an iCycler (Bio-Rad Laboratories). cDNA was prepared as described above, and assayed in triplicate. .. Each 25 μ l reaction contained 12.5 μ l of 2× SYBR Green supermix (Bio-Rad Laboratories), 0.4 μ M of each primer and 1 μ l of template.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Notch-Mediated Suppression of TSC2 Expression Regulates Cell Differentiation in the Drosophila Intestinal Stem Cell Lineage
Article Snippet: .. Reverse transcription and real-time PCR Total RNA was extracted using Trizol. cDNAs were synthesized using oligo-dT primers and real-time RTPCR was performed on a BioRad iQ5 detection system (using SYBR Green and ΔΔCt quantification method). .. Gigas and Suppressor of Hairless expression levels were quantified relative to Actin5c expression.

Generated:

Article Title: RALFL34 regulates formative cell divisions in Arabidopsis pericycle during lateral root initiation
Article Snippet: .. Quantitative real-time PCR (qRT-PCR) Total RNA was extracted from lines of interest using an RNeasy kit (Qiagen) as per the manufacturer’s instructions. cDNA was generated from 1 µg or 200ng of total RNA via oligo(dT) primers using iScript (BioRad) or Superscript II (Invitrogen), respectively, as per the instructions. .. Quantitative PCRs were performed in at least two technical repeats, using the SYBR Green QPCR Master Mix (Quanta Biosciences), in our modified reaction protocol: Master mix, 7 μl per reaction [forward primer (100 μM), 0.1 μl per reaction; reverse primer (100 μM), 0.1 μl per reaction; SYBR (2×), 6 μl per reaction; dH2 O, 0.8 μl per reaction] and template cDNA (diluted 1/100 in dH2 O, 5 μl per reaction.

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    Bio-Rad oligo dt
    Expression of human miRNAs suppress HIV-1 replication in cell culture. ( A ) Effects of transfection of the indicated individual miRNAs on HIV-1 replication in cultured cells. The 42CD4 cells were transfected with the indicated miRNAs, and cells were infected with HIV-1 NL4-3 24 h later. Viral replication was monitored by measuring RT production in culture supernatants, 3 days after infection. Values are expressed as percent of a control irrelevant <t>RNA</t> oligonucleotide <t>(oligo</t> fluo). Arrows indicate miRNA chosen for follow-up experiments. ( B ) Time course replication curves spanning 2, 3, 4 and 6 days post-infection in cells transfected with miR-138, miR-149, miR-326, miR-195 and miR-29b (indicated by an arrow in panel A) and irrelevant control RNA oligonucleotide (oligo fluo); miR-29b is a positive control; oligo fluo is a negative control. Results are representative of three independent experiments. Values are the mean of three separate experiments with standard deviations.
    Oligo Dt, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo dt/product/Bio-Rad
    Average 94 stars, based on 98 article reviews
    Price from $9.99 to $1999.99
    oligo dt - by Bioz Stars, 2020-11
    94/100 stars
      Buy from Supplier

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    Expression of human miRNAs suppress HIV-1 replication in cell culture. ( A ) Effects of transfection of the indicated individual miRNAs on HIV-1 replication in cultured cells. The 42CD4 cells were transfected with the indicated miRNAs, and cells were infected with HIV-1 NL4-3 24 h later. Viral replication was monitored by measuring RT production in culture supernatants, 3 days after infection. Values are expressed as percent of a control irrelevant RNA oligonucleotide (oligo fluo). Arrows indicate miRNA chosen for follow-up experiments. ( B ) Time course replication curves spanning 2, 3, 4 and 6 days post-infection in cells transfected with miR-138, miR-149, miR-326, miR-195 and miR-29b (indicated by an arrow in panel A) and irrelevant control RNA oligonucleotide (oligo fluo); miR-29b is a positive control; oligo fluo is a negative control. Results are representative of three independent experiments. Values are the mean of three separate experiments with standard deviations.

    Journal: Nucleic Acids Research

    Article Title: The extent of sequence complementarity correlates with the potency of cellular miRNA-mediated restriction of HIV-1

    doi: 10.1093/nar/gks912

    Figure Lengend Snippet: Expression of human miRNAs suppress HIV-1 replication in cell culture. ( A ) Effects of transfection of the indicated individual miRNAs on HIV-1 replication in cultured cells. The 42CD4 cells were transfected with the indicated miRNAs, and cells were infected with HIV-1 NL4-3 24 h later. Viral replication was monitored by measuring RT production in culture supernatants, 3 days after infection. Values are expressed as percent of a control irrelevant RNA oligonucleotide (oligo fluo). Arrows indicate miRNA chosen for follow-up experiments. ( B ) Time course replication curves spanning 2, 3, 4 and 6 days post-infection in cells transfected with miR-138, miR-149, miR-326, miR-195 and miR-29b (indicated by an arrow in panel A) and irrelevant control RNA oligonucleotide (oligo fluo); miR-29b is a positive control; oligo fluo is a negative control. Results are representative of three independent experiments. Values are the mean of three separate experiments with standard deviations.

    Article Snippet: Reverse transcription was performed on 0.5 µg of cellular RNA with oligo(dT) as the RT primer, and quantitative PCR for HIV RNAs was performed with 1% of the RT reaction using IQ SYBR Green Supermix (Bio-Rad), and the reaction was carried out with a Bio-Rad CFX96 Real-Time System instrument.

    Techniques: Expressing, Cell Culture, Transfection, Infection, Positive Control, Negative Control