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Promega oligo dt chromatography
: localisation of <t>mRNA.</t> (A) Fluorescent in situ hybridisation assay using specific <t>oligo</t> (dT) probe. As the control, RNase A was incubated with the parasites before probe hybridisation. DAPI: DNA stained with DAPI; mRNA: mRNA detection; granules: labelling of pixels with high intensity fluorescence signals; N: nucleus; K: kinetoplast. Scale bar = 5 µm. (B) Histogram of mRNA granule counts per cell.
Oligo Dt Chromatography, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oligo dt chromatography/product/Promega
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
oligo dt chromatography - by Bioz Stars, 2020-09
92/100 stars

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1) Product Images from "Trypanosoma cruzi transcriptome during axenic epimastigote growth curve"

Article Title: Trypanosoma cruzi transcriptome during axenic epimastigote growth curve

Journal: Memórias do Instituto Oswaldo Cruz

doi: 10.1590/0074-02760170404

: localisation of mRNA. (A) Fluorescent in situ hybridisation assay using specific oligo (dT) probe. As the control, RNase A was incubated with the parasites before probe hybridisation. DAPI: DNA stained with DAPI; mRNA: mRNA detection; granules: labelling of pixels with high intensity fluorescence signals; N: nucleus; K: kinetoplast. Scale bar = 5 µm. (B) Histogram of mRNA granule counts per cell.
Figure Legend Snippet: : localisation of mRNA. (A) Fluorescent in situ hybridisation assay using specific oligo (dT) probe. As the control, RNase A was incubated with the parasites before probe hybridisation. DAPI: DNA stained with DAPI; mRNA: mRNA detection; granules: labelling of pixels with high intensity fluorescence signals; N: nucleus; K: kinetoplast. Scale bar = 5 µm. (B) Histogram of mRNA granule counts per cell.

Techniques Used: In Situ, Hybridization, Incubation, Staining, Fluorescence

2) Product Images from "Trypanosoma cruzi transcriptome during axenic epimastigote growth curve"

Article Title: Trypanosoma cruzi transcriptome during axenic epimastigote growth curve

Journal: Memórias do Instituto Oswaldo Cruz

doi: 10.1590/0074-02760170404

: localisation of mRNA. (A) Fluorescent in situ hybridisation assay using specific oligo (dT) probe. As the control, RNase A was incubated with the parasites before probe hybridisation. DAPI: DNA stained with DAPI; mRNA: mRNA detection; granules: labelling of pixels with high intensity fluorescence signals; N: nucleus; K: kinetoplast. Scale bar = 5 µm. (B) Histogram of mRNA granule counts per cell.
Figure Legend Snippet: : localisation of mRNA. (A) Fluorescent in situ hybridisation assay using specific oligo (dT) probe. As the control, RNase A was incubated with the parasites before probe hybridisation. DAPI: DNA stained with DAPI; mRNA: mRNA detection; granules: labelling of pixels with high intensity fluorescence signals; N: nucleus; K: kinetoplast. Scale bar = 5 µm. (B) Histogram of mRNA granule counts per cell.

Techniques Used: In Situ, Hybridization, Incubation, Staining, Fluorescence

Related Articles

Isolation:

Article Title: Trypanosoma cruzi transcriptome during axenic epimastigote growth curve
Article Snippet: .. Subsequently, RNA integrity and quality was analysed using the Agilent 2100 bioanalyzer and the RNA 6000 Pico Kit (Agilent). mRNA was selected by oligo-dT chromatography using the PolyATtract® mRNA Isolation Kit (Promega). .. RNA-Seq - Samples were prepared for RNA sequencing using the SOLiDTM Total RNA-Seq Kit (#4445374; Life Technologies) according to the manufacturer’s instructions.

Chromatography:

Article Title: Trypanosoma cruzi transcriptome during axenic epimastigote growth curve
Article Snippet: .. Subsequently, RNA integrity and quality was analysed using the Agilent 2100 bioanalyzer and the RNA 6000 Pico Kit (Agilent). mRNA was selected by oligo-dT chromatography using the PolyATtract® mRNA Isolation Kit (Promega). .. RNA-Seq - Samples were prepared for RNA sequencing using the SOLiDTM Total RNA-Seq Kit (#4445374; Life Technologies) according to the manufacturer’s instructions.

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  • 92
    Promega oligo dt chromatography
    : localisation of <t>mRNA.</t> (A) Fluorescent in situ hybridisation assay using specific <t>oligo</t> (dT) probe. As the control, RNase A was incubated with the parasites before probe hybridisation. DAPI: DNA stained with DAPI; mRNA: mRNA detection; granules: labelling of pixels with high intensity fluorescence signals; N: nucleus; K: kinetoplast. Scale bar = 5 µm. (B) Histogram of mRNA granule counts per cell.
    Oligo Dt Chromatography, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo dt chromatography/product/Promega
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    oligo dt chromatography - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    94
    Promega oligo dt primers
    : localisation of <t>mRNA.</t> (A) Fluorescent in situ hybridisation assay using specific <t>oligo</t> (dT) probe. As the control, RNase A was incubated with the parasites before probe hybridisation. DAPI: DNA stained with DAPI; mRNA: mRNA detection; granules: labelling of pixels with high intensity fluorescence signals; N: nucleus; K: kinetoplast. Scale bar = 5 µm. (B) Histogram of mRNA granule counts per cell.
    Oligo Dt Primers, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo dt primers/product/Promega
    Average 94 stars, based on 241 article reviews
    Price from $9.99 to $1999.99
    oligo dt primers - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    88
    Promega oligo dt cellulose chromatography
    : localisation of <t>mRNA.</t> (A) Fluorescent in situ hybridisation assay using specific <t>oligo</t> (dT) probe. As the control, RNase A was incubated with the parasites before probe hybridisation. DAPI: DNA stained with DAPI; mRNA: mRNA detection; granules: labelling of pixels with high intensity fluorescence signals; N: nucleus; K: kinetoplast. Scale bar = 5 µm. (B) Histogram of mRNA granule counts per cell.
    Oligo Dt Cellulose Chromatography, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo dt cellulose chromatography/product/Promega
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    oligo dt cellulose chromatography - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    : localisation of mRNA. (A) Fluorescent in situ hybridisation assay using specific oligo (dT) probe. As the control, RNase A was incubated with the parasites before probe hybridisation. DAPI: DNA stained with DAPI; mRNA: mRNA detection; granules: labelling of pixels with high intensity fluorescence signals; N: nucleus; K: kinetoplast. Scale bar = 5 µm. (B) Histogram of mRNA granule counts per cell.

    Journal: Memórias do Instituto Oswaldo Cruz

    Article Title: Trypanosoma cruzi transcriptome during axenic epimastigote growth curve

    doi: 10.1590/0074-02760170404

    Figure Lengend Snippet: : localisation of mRNA. (A) Fluorescent in situ hybridisation assay using specific oligo (dT) probe. As the control, RNase A was incubated with the parasites before probe hybridisation. DAPI: DNA stained with DAPI; mRNA: mRNA detection; granules: labelling of pixels with high intensity fluorescence signals; N: nucleus; K: kinetoplast. Scale bar = 5 µm. (B) Histogram of mRNA granule counts per cell.

    Article Snippet: Subsequently, RNA integrity and quality was analysed using the Agilent 2100 bioanalyzer and the RNA 6000 Pico Kit (Agilent). mRNA was selected by oligo-dT chromatography using the PolyATtract® mRNA Isolation Kit (Promega).

    Techniques: In Situ, Hybridization, Incubation, Staining, Fluorescence

    : localisation of mRNA. (A) Fluorescent in situ hybridisation assay using specific oligo (dT) probe. As the control, RNase A was incubated with the parasites before probe hybridisation. DAPI: DNA stained with DAPI; mRNA: mRNA detection; granules: labelling of pixels with high intensity fluorescence signals; N: nucleus; K: kinetoplast. Scale bar = 5 µm. (B) Histogram of mRNA granule counts per cell.

    Journal: Memórias do Instituto Oswaldo Cruz

    Article Title: Trypanosoma cruzi transcriptome during axenic epimastigote growth curve

    doi: 10.1590/0074-02760170404

    Figure Lengend Snippet: : localisation of mRNA. (A) Fluorescent in situ hybridisation assay using specific oligo (dT) probe. As the control, RNase A was incubated with the parasites before probe hybridisation. DAPI: DNA stained with DAPI; mRNA: mRNA detection; granules: labelling of pixels with high intensity fluorescence signals; N: nucleus; K: kinetoplast. Scale bar = 5 µm. (B) Histogram of mRNA granule counts per cell.

    Article Snippet: Subsequently, RNA integrity and quality was analysed using the Agilent 2100 bioanalyzer and the RNA 6000 Pico Kit (Agilent). mRNA was selected by oligo-dT chromatography using the PolyATtract® mRNA Isolation Kit (Promega).

    Techniques: In Situ, Hybridization, Incubation, Staining, Fluorescence