oligo dt 18 primer  (Thermo Fisher)


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    Name:
    Oligo dT 18 Primer
    Description:
    Thermo Scientific Oligo dT 18 Primer is a synthetic single stranded 18 mer oligonucleotide with 5 and 3 hydroxyl ends The primer is supplied as a ready to use 20X concentrated aqueous solution Applications• cDNA Synthesis• RT qPCR• RT PCRRelated ProductsAnchored Oligo dTRandom Hexamer PrimerOligo dT 18 Primer
    Catalog Number:
    so131
    Price:
    None
    Applications:
    Cloning|One-Step qRT-PCR|PCR & Real-Time PCR|RT-PCR|Real Time PCR (qPCR)|Reverse Transcription|Two-Step RT-PCR|cDNA Libraries & Library Construction
    Category:
    Oligos Primers Probes Nucleotides
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    Structured Review

    Thermo Fisher oligo dt 18 primer
    Thermo Scientific Oligo dT 18 Primer is a synthetic single stranded 18 mer oligonucleotide with 5 and 3 hydroxyl ends The primer is supplied as a ready to use 20X concentrated aqueous solution Applications• cDNA Synthesis• RT qPCR• RT PCRRelated ProductsAnchored Oligo dTRandom Hexamer PrimerOligo dT 18 Primer
    https://www.bioz.com/result/oligo dt 18 primer/product/Thermo Fisher
    Average 99 stars, based on 94 article reviews
    Price from $9.99 to $1999.99
    oligo dt 18 primer - by Bioz Stars, 2020-11
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Low dose of alcohol attenuates pro-atherosclerotic activity of thrombin.
    Article Snippet: .. Thrombin, the active enzyme of the coagulation system, plays a critical role in the pathogenesis of atherosclerosis. .. Thrombin, the active enzyme of the coagulation system, plays a critical role in the pathogenesis of atherosclerosis.

    Synthesized:

    Article Title: MUC4 Mucin Interacts with and Stabilizes the HER2 Oncoprotein in Human Pancreatic Cancer Cells
    Article Snippet: .. RNA isolation and reverse transcription-PCR analysis Total RNA was isolated from cell lines as previously described ( ). cDNA was synthesized using 2 μg of total RNA, oligo(dT)18 primer, and Superscript reverse transcriptase (Invitrogen Corp.). .. Quantitative real-time PCR was performed using 2 μL of a 1:50 dilution of first-strand cDNA in FastStart DNA Master SYBR Green I (Roche Applied System) using primers specific to HER2 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in iCycler (Bio-Rad).

    Isolation:

    Article Title: MUC4 Mucin Interacts with and Stabilizes the HER2 Oncoprotein in Human Pancreatic Cancer Cells
    Article Snippet: .. RNA isolation and reverse transcription-PCR analysis Total RNA was isolated from cell lines as previously described ( ). cDNA was synthesized using 2 μg of total RNA, oligo(dT)18 primer, and Superscript reverse transcriptase (Invitrogen Corp.). .. Quantitative real-time PCR was performed using 2 μL of a 1:50 dilution of first-strand cDNA in FastStart DNA Master SYBR Green I (Roche Applied System) using primers specific to HER2 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in iCycler (Bio-Rad).

    Quantitative RT-PCR:

    Article Title: Selection and validation of reference genes for quantitative RT-PCR expression studies of the non-model crop Musa
    Article Snippet: .. Two-step real-time RT-PCR One microgram of each DNA-free RNA sample was reverse-transcribed to cDNA by using an oligo(dT)18 primer and the RevertAid H Minus First Strand cDNA Synthesis Kit (Fermentas, St-Leon Rot, Germany) according to the manufacturer’s instructions. .. Real-time RT-PCR was performed on the Corbett Rotor-Gene 3000 (Qiagen, Hilden, Germany) using the SYBR Green I technology.

    Purification:

    Article Title: Development and characterization of 5 canine B-cell lymphoma cell lines
    Article Snippet: .. mRNA was purified from each cell line using TRIzol Reagent (Invitrogen), and then transcribed using oligo(dT)18 primer (Fermentas) and M-MLV reverse transcriptase (Promega). .. The upper p53 fragment (nt 18–932) was amplified from cDNA with primers 5′AAGTCCAGAGCCACCATCC and 5′TCAAAGCTGTTGCGTCCC, and the lower fragment (nt 727–1362) was amplified with primers 5′-GCCAAGTACCTGGACGACA and 5′-CAGGGAAGGAGGACGAGA.

    Random Hexamer Labeling:

    Article Title: Immunomodulatory sphingosine-1-phosphates as plasma biomarkers of Alzheimer’s disease and vascular cognitive impairment
    Article Snippet: .. Approximately 1 μg of each sample was primed with oligo (dT)18 and random hexamer primers prior using Maxima First Strand cDNA synthesis kit. .. For quantitative real-time RT-PCR, targets were amplified with Maxima SYBR Green/ROX qPCR Master Mix on an Applied Biosystems ViiA 7 Real-Time PCR System (Foster City, CA, USA) using gene-specific primer pairs (IL1β: forward 5′-GGA GAA TGA CCT GAG CAC CT-3′; reverse 5′-GGA GGT GGA GAG CTT TCA GT-3′, IL-6: forward 5′-AGT CCT GAT CCA GTT CCT GC-3′; reverse 5′-AAG CTG CGC AGA ATG AGA TG-3′, with normalization to the housekeeping GAPDH: forward 5′-CGA CCA CTT TGT CAA GCT CA-3′; reverse 5′-AGG GGT CTA CAT GGC AAC TG-3′).

    Polymerase Chain Reaction:

    Article Title: Expression and evolutionary divergence of the non-conventional olfactory receptor in four species of fig wasp associated with one species of fig
    Article Snippet: .. Each experiment used about 3 μg RNA for first-strand cDNA synthesis with EasyScript Reverse Transcriptase (Transgen Biotech) and Oligo(dT)18 primers (Invitrogen) to generate templates for individual PCR reactions. ..

    Article Title: Low dose of alcohol attenuates pro-atherosclerotic activity of thrombin.
    Article Snippet: .. Thrombin, the active enzyme of the coagulation system, plays a critical role in the pathogenesis of atherosclerosis. .. Thrombin, the active enzyme of the coagulation system, plays a critical role in the pathogenesis of atherosclerosis.

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  • 99
    Thermo Fisher oligo dt 18
    Oligo Dt 18, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3025 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo dt 18/product/Thermo Fisher
    Average 99 stars, based on 3025 article reviews
    Price from $9.99 to $1999.99
    oligo dt 18 - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

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