odyssey blocking buffer (LI-COR)
86
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Name:
Odyssey Blocking Buffer PBS
Description:
Non mammalian Odyssey Blocker This Blocking Reagent is in PBS phosphate buffered saline containing 0 1 sodium azide
Catalog Number:
927-40000
Price:
None
Category:
Blocking buffer
Applications:
Western blot, In-Cell Western assay
Size:
1
Quantity:
1
|
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Structured Review
LI-COR
odyssey blocking buffer
Non mammalian Odyssey Blocker This Blocking Reagent is in PBS phosphate buffered saline containing 0 1 sodium azide
https://www.bioz.com/result/odyssey blocking buffer/product/LI-COR
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Non mammalian Odyssey Blocker This Blocking Reagent is in PBS phosphate buffered saline containing 0 1 sodium azide
https://www.bioz.com/result/odyssey blocking buffer/product/LI-COR
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
odyssey blocking buffer - by Bioz Stars,
2021-03
86/100 stars
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Related Articles
Blocking Assay:Article Title: Homology-guided identification of a conserved motif linking the antiviral functions of IFITM3 to its oligomeric state Article Snippet: Proteins were transferred Amersham Protran Premium Nitrocellulose Membrane, pore size 0.20 μm (GE Healthcare). .. Membranes were blocked with Odyssey blocking Article Title: Novel exosome-targeted T-cell-based vaccine counteracts T-cell anergy and converts CTL exhaustion in chronic infection via CD40L signaling through the mTORC1 pathway Article Snippet: Cell lysates (10 μg/lane) were loaded onto 12% acrylamide gels, subjected to sodium dodecyl sulfate-poly-acrylamide gel electrophoresis (SDS-PAGE), and subsequently transferred onto a nitrocellulose membrane (Millipore, Bedford, MA, USA). .. The membrane was blocked by incubation for 2 h at room temperature with Article Title: Coordination of asparagine uptake and asparagine synthetase expression is required for T cell activation Article Snippet: Membranes were blocked in LI-COR blocking buffer (LI-COR Biosciences). .. Primary antibodies (rabbit anti-ASNS – HPA029318, Atlas Antibodies, mouse anti-β actin, Article Title: Crystal structure of Spot 14, a modulator of fatty acid synthesis Article Snippet: .. The membranes were incubated with blocking solution containing 5% nonfat dried milk and 5% newborn calf serum in PBST or Article Title: Neuraminidase inhibition contributes to influenza A virus neutralization by anti-hemagglutinin stem antibodies Article Snippet: The following day we washed cells with DPBS twice and added virus diluted in infection media (MEM medium [Gibco] containing 0.3% BSA [fraction V; Roche], 10 mM HEPES [Corning], 500 µg/ml gentamicin [Quality Biologicals], and 1 µg/ml TPCK trypsin [Worthington]). .. At the times p.i. indicated, we fix-permeabilized the cells with 100% ice-cold methanol for 20 min. We washed the cells with DPBS and added 30 µl HB65 anti-NP mAb or/and NA2-1C1 anti-NA mAb (1 µg/ml) diluted in Incubation:Article Title: Homology-guided identification of a conserved motif linking the antiviral functions of IFITM3 to its oligomeric state Article Snippet: Proteins were transferred Amersham Protran Premium Nitrocellulose Membrane, pore size 0.20 μm (GE Healthcare). .. Membranes were blocked with Odyssey blocking Article Title: Novel exosome-targeted T-cell-based vaccine counteracts T-cell anergy and converts CTL exhaustion in chronic infection via CD40L signaling through the mTORC1 pathway Article Snippet: Cell lysates (10 μg/lane) were loaded onto 12% acrylamide gels, subjected to sodium dodecyl sulfate-poly-acrylamide gel electrophoresis (SDS-PAGE), and subsequently transferred onto a nitrocellulose membrane (Millipore, Bedford, MA, USA). .. The membrane was blocked by incubation for 2 h at room temperature with Article Title: Th Cells Promote CTL Survival and Memory via Acquired pMHC-I and Endogenous IL-2 and CD40L Signaling and by Modulating Apoptosis-Controlling Pathways Article Snippet: The blots were stained with panel of monoclonal- or polyclonal-rabbit Abs specific for Bcl-2 (50E3), β-actin (13E5), Bcl10 (C79F1), Akt1 (2H10), phospho-Akt1 (S473), cleaved Caspase-3 (Asp175) and -7 (Asp198) (Cell Signaling Technology), NF-κB-p65 (C-20), phospho-NF-κB-p65 (Ser536) (Santa Cruz Biotechnology) and NFATc1 (7A6) (BD Biosciences). .. The blots were incubated with goat anti-rabbit IRDyeR800/680CW, and band densities were quantified using Article Title: Crystal structure of Spot 14, a modulator of fatty acid synthesis Article Snippet: .. The membranes were incubated with blocking solution containing 5% nonfat dried milk and 5% newborn calf serum in PBST or In-Cell ELISA:Article Title: Inhibition of citrate cotransporter Slc13a5/mINDY by RNAi improves hepatic insulin sensitivity and prevents diet-induced non-alcoholic fatty liver disease in mice Article Snippet: 2.3.7 In-Cell Western Assay HEK293-mINDY cells were reversely transfected in a 96-well format with RNAiMAX as described in the section “siRNA transfection”. .. 48 h post-transfection, cells were fixed with 3.7% formaldehyde and the Labeling:Article Title: Structural insights into non-covalent ubiquitin activation of the cIAP1-UbcH5B∼ubiquitin complex Article Snippet: Reactions performed using 32 P-Ub were dried and visualized using autoradiography. .. Fluorescently labeled UbcH5B∼Ub was visualized by a Staining:Article Title: Structural insights into non-covalent ubiquitin activation of the cIAP1-UbcH5B∼ubiquitin complex Article Snippet: Reactions performed using 32 P-Ub were dried and visualized using autoradiography. .. Fluorescently labeled UbcH5B∼Ub was visualized by a |
