odyssey blocking buffer  (LI-COR)

 
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    Name:
    Odyssey Blocking Buffer PBS
    Description:
    Non mammalian Odyssey Blocker This Blocking Reagent is in PBS phosphate buffered saline containing 0 1 sodium azide
    Catalog Number:
    927-40000
    Price:
    None
    Category:
    Blocking buffer
    Applications:
    Western blot, In-Cell Western assay
    Size:
    1
    Quantity:
    1
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    Structured Review

    LI-COR odyssey blocking buffer
    Odyssey Blocking Buffer PBS
    Non mammalian Odyssey Blocker This Blocking Reagent is in PBS phosphate buffered saline containing 0 1 sodium azide
    https://www.bioz.com/result/odyssey blocking buffer/product/LI-COR
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    odyssey blocking buffer - by Bioz Stars, 2021-03
    86/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Homology-guided identification of a conserved motif linking the antiviral functions of IFITM3 to its oligomeric state
    Article Snippet: Proteins were transferred Amersham Protran Premium Nitrocellulose Membrane, pore size 0.20 μm (GE Healthcare). .. Membranes were blocked with Odyssey blocking buffer in PBS (Li-COR) and incubated with anti-FLAG M2 (F1804; Sigma) and anti-c-Myc (C3956; Sigma). .. Secondary antibodies conjugated to DyLight 800 or 680 (Li-COR) and the Li-COR Odyssey imaging system were used to reveal specific protein detection.

    Article Title: Novel exosome-targeted T-cell-based vaccine counteracts T-cell anergy and converts CTL exhaustion in chronic infection via CD40L signaling through the mTORC1 pathway
    Article Snippet: Cell lysates (10 μg/lane) were loaded onto 12% acrylamide gels, subjected to sodium dodecyl sulfate-poly-acrylamide gel electrophoresis (SDS-PAGE), and subsequently transferred onto a nitrocellulose membrane (Millipore, Bedford, MA, USA). .. The membrane was blocked by incubation for 2 h at room temperature with ODYSSEY blocking buffer (LI-COR Bioscience, Lincoln, NE, USA) and immunoblotted with Abs specific for GRAIL and ITCH at 4 °C overnight. .. After three washes with PBS containing 0.05% (V/V) Tween 20, the membrane was further incubated with goat anti-rabbit IRDye800CW and scanned using an ODYSSEY instrument according to the manufacturer’s instructions (LI-COR Bioscience).

    Article Title: Coordination of asparagine uptake and asparagine synthetase expression is required for T cell activation
    Article Snippet: Membranes were blocked in LI-COR blocking buffer (LI-COR Biosciences). .. Primary antibodies (rabbit anti-ASNS – HPA029318, Atlas Antibodies, mouse anti-β actin, Clone AC-15, Sigma Aldrich) were diluted in LI-COR blocking buffer and detected using goat anti-rabbit-AF680 and goat anti-mouse-AF790 secondary reagents (Molecular Probes). .. Protein bands were detected and quantified using a Li-COR Odyssey Imaging System.

    Article Title: Crystal structure of Spot 14, a modulator of fatty acid synthesis
    Article Snippet: .. The membranes were incubated with blocking solution containing 5% nonfat dried milk and 5% newborn calf serum in PBST or LI-COR blocking buffer for 30 min. Immunoblot analyses were performed using rabbit polyclonal antibodies against rat ACC1, mouse MIG12 , and a mouse monoclonal antibody against S14 (Roche). .. Horseradish peroxidase linked anti-rabbit IgG (GE Healthcare) and IRDye 800-conjugated anti-rabbit antibody (LI-COR) were used as secondary antibodies to visualize ACC or MIG12.

    Article Title: Neuraminidase inhibition contributes to influenza A virus neutralization by anti-hemagglutinin stem antibodies
    Article Snippet: The following day we washed cells with DPBS twice and added virus diluted in infection media (MEM medium [Gibco] containing 0.3% BSA [fraction V; Roche], 10 mM HEPES [Corning], 500 µg/ml gentamicin [Quality Biologicals], and 1 µg/ml TPCK trypsin [Worthington]). .. At the times p.i. indicated, we fix-permeabilized the cells with 100% ice-cold methanol for 20 min. We washed the cells with DPBS and added 30 µl HB65 anti-NP mAb or/and NA2-1C1 anti-NA mAb (1 µg/ml) diluted in Odyssey Blocking Buffer PBS (OBB; Li-Cor) for 1 h at room temperature. .. We washed the plate with DPBS containing 0.05% NP-40 (Thermo Fisher) twice and added IRDye 800CW goat anti-mouse or IRDye 680RD goat anti-rabbit secondary Ab (Li-Cor) 1,000× diluted in OBB.

    Incubation:

    Article Title: Homology-guided identification of a conserved motif linking the antiviral functions of IFITM3 to its oligomeric state
    Article Snippet: Proteins were transferred Amersham Protran Premium Nitrocellulose Membrane, pore size 0.20 μm (GE Healthcare). .. Membranes were blocked with Odyssey blocking buffer in PBS (Li-COR) and incubated with anti-FLAG M2 (F1804; Sigma) and anti-c-Myc (C3956; Sigma). .. Secondary antibodies conjugated to DyLight 800 or 680 (Li-COR) and the Li-COR Odyssey imaging system were used to reveal specific protein detection.

    Article Title: Novel exosome-targeted T-cell-based vaccine counteracts T-cell anergy and converts CTL exhaustion in chronic infection via CD40L signaling through the mTORC1 pathway
    Article Snippet: Cell lysates (10 μg/lane) were loaded onto 12% acrylamide gels, subjected to sodium dodecyl sulfate-poly-acrylamide gel electrophoresis (SDS-PAGE), and subsequently transferred onto a nitrocellulose membrane (Millipore, Bedford, MA, USA). .. The membrane was blocked by incubation for 2 h at room temperature with ODYSSEY blocking buffer (LI-COR Bioscience, Lincoln, NE, USA) and immunoblotted with Abs specific for GRAIL and ITCH at 4 °C overnight. .. After three washes with PBS containing 0.05% (V/V) Tween 20, the membrane was further incubated with goat anti-rabbit IRDye800CW and scanned using an ODYSSEY instrument according to the manufacturer’s instructions (LI-COR Bioscience).

    Article Title: Th Cells Promote CTL Survival and Memory via Acquired pMHC-I and Endogenous IL-2 and CD40L Signaling and by Modulating Apoptosis-Controlling Pathways
    Article Snippet: The blots were stained with panel of monoclonal- or polyclonal-rabbit Abs specific for Bcl-2 (50E3), β-actin (13E5), Bcl10 (C79F1), Akt1 (2H10), phospho-Akt1 (S473), cleaved Caspase-3 (Asp175) and -7 (Asp198) (Cell Signaling Technology), NF-κB-p65 (C-20), phospho-NF-κB-p65 (Ser536) (Santa Cruz Biotechnology) and NFATc1 (7A6) (BD Biosciences). .. The blots were incubated with goat anti-rabbit IRDyeR800/680CW, and band densities were quantified using ODYSSEY densitometer (LI-COR Bioscience). ..

    Article Title: Crystal structure of Spot 14, a modulator of fatty acid synthesis
    Article Snippet: .. The membranes were incubated with blocking solution containing 5% nonfat dried milk and 5% newborn calf serum in PBST or LI-COR blocking buffer for 30 min. Immunoblot analyses were performed using rabbit polyclonal antibodies against rat ACC1, mouse MIG12 , and a mouse monoclonal antibody against S14 (Roche). .. Horseradish peroxidase linked anti-rabbit IgG (GE Healthcare) and IRDye 800-conjugated anti-rabbit antibody (LI-COR) were used as secondary antibodies to visualize ACC or MIG12.

    In-Cell ELISA:

    Article Title: Inhibition of citrate cotransporter Slc13a5/mINDY by RNAi improves hepatic insulin sensitivity and prevents diet-induced non-alcoholic fatty liver disease in mice
    Article Snippet: 2.3.7 In-Cell Western Assay HEK293-mINDY cells were reversely transfected in a 96-well format with RNAiMAX as described in the section “siRNA transfection”. .. 48 h post-transfection, cells were fixed with 3.7% formaldehyde and the Odyssey In-Cell Western Assay (LiCor, Bad Homburg, Germany) was performed according to the manufacturer's instructions using an anti-FLAG M2 antibody (Sigma) overnight for detection of FLAG-tagged mINDY. .. Subsequently, the plate was incubated with a fluorescently labeled secondary goat-anti-mouse antibody (IRDye 800 CW, LiCor) and the CellTag 700 Stain (LiCor) for normalization to cell number.

    Labeling:

    Article Title: Structural insights into non-covalent ubiquitin activation of the cIAP1-UbcH5B∼ubiquitin complex
    Article Snippet: Reactions performed using 32 P-Ub were dried and visualized using autoradiography. .. Fluorescently labeled UbcH5B∼Ub was visualized by a LI-COR Odyssey scanner, followed by staining with InstantBlue. .. SPR All SPR experiments were performed at 25 °C on a Biacore T200 system with a CM-5 chip.

    Staining:

    Article Title: Structural insights into non-covalent ubiquitin activation of the cIAP1-UbcH5B∼ubiquitin complex
    Article Snippet: Reactions performed using 32 P-Ub were dried and visualized using autoradiography. .. Fluorescently labeled UbcH5B∼Ub was visualized by a LI-COR Odyssey scanner, followed by staining with InstantBlue. .. SPR All SPR experiments were performed at 25 °C on a Biacore T200 system with a CM-5 chip.

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  • 97
    LI-COR li cor odyssey scanner
    Ub B stimulates cIAP1R-catalyzed Ub transfer. A , nonreduced autoradiograms of lysine discharge reactions showing the disappearance of UbcH5B variant∼ 32 P-Ub over time in the presence and absence of UbΔGG (300 μ m ) catalyzed by cIAP1R. B , nonreduced SDS-PAGE showing the cIAP1R-mediated discharge of <t>fluorescently</t> labeled UbcH5B∼Ub to l -lysine over time in the presence of UbΔGG (20 μ m ) or UbcH5B S22R,F62A,P95D–Ub (20 μ m ) visualized with a <t>LI-COR</t> Odyssey scanner ( top ) followed by staining with InstantBlue ( bottom ). *, fluorescently labeled Ub.
    Li Cor Odyssey Scanner, supplied by LI-COR, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/li cor odyssey scanner/product/LI-COR
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    li cor odyssey scanner - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    Image Search Results


    Ub B stimulates cIAP1R-catalyzed Ub transfer. A , nonreduced autoradiograms of lysine discharge reactions showing the disappearance of UbcH5B variant∼ 32 P-Ub over time in the presence and absence of UbΔGG (300 μ m ) catalyzed by cIAP1R. B , nonreduced SDS-PAGE showing the cIAP1R-mediated discharge of fluorescently labeled UbcH5B∼Ub to l -lysine over time in the presence of UbΔGG (20 μ m ) or UbcH5B S22R,F62A,P95D–Ub (20 μ m ) visualized with a LI-COR Odyssey scanner ( top ) followed by staining with InstantBlue ( bottom ). *, fluorescently labeled Ub.

    Journal: The Journal of Biological Chemistry

    Article Title: Structural insights into non-covalent ubiquitin activation of the cIAP1-UbcH5B∼ubiquitin complex

    doi: 10.1074/jbc.RA118.006045

    Figure Lengend Snippet: Ub B stimulates cIAP1R-catalyzed Ub transfer. A , nonreduced autoradiograms of lysine discharge reactions showing the disappearance of UbcH5B variant∼ 32 P-Ub over time in the presence and absence of UbΔGG (300 μ m ) catalyzed by cIAP1R. B , nonreduced SDS-PAGE showing the cIAP1R-mediated discharge of fluorescently labeled UbcH5B∼Ub to l -lysine over time in the presence of UbΔGG (20 μ m ) or UbcH5B S22R,F62A,P95D–Ub (20 μ m ) visualized with a LI-COR Odyssey scanner ( top ) followed by staining with InstantBlue ( bottom ). *, fluorescently labeled Ub.

    Article Snippet: Fluorescently labeled UbcH5B∼Ub was visualized by a LI-COR Odyssey scanner, followed by staining with InstantBlue.

    Techniques: Variant Assay, SDS Page, Labeling, Staining

    T cell development and homeostasis is unimpaired in ASNS-deficient mice (A) Western blots show levels of ASNS protein in lysates of OT-1 T cells stimulated in IMDM with SIINFEKL peptide ± rapamycin (rap), for the indicated time periods, or thymocytes. (B) ASNS levels in lysates from activated lymph node T cell from homozygous, heterozygous or wild-type (WT) Asns Tm1a mice. For (A, B) β actin serves as a protein loading control. Values in bar graph represent relative ASNS expression levels (ASNS/Actin) calculated using the LI-COR Odyssey imaging system from 1 of 2 repeated experiments. (C) Thymocyte and lymph node (LN) cell populations from littermate Asns Tm1a mice were analysed by flow cytometry. Total thymocytes (D) and LN CD4 and CD8 T cells (E) were enumerated from 3-4 age-matched male mice of each genotype. (F) Proportions of naïve, effector and memory cells within gated CD4 + and CD8 + T cell populations were analysed by analysis of CD44 and CD62L expression by flow cytometry. FACS dotplots are representative of 5-6 mice of each genotype. Values on dotplots represent % cells within the defined gates. NS – not significant, as determined by one-way ANOVA.

    Journal: bioRxiv

    Article Title: Coordination of asparagine uptake and asparagine synthetase expression is required for T cell activation

    doi: 10.1101/2020.02.28.969774

    Figure Lengend Snippet: T cell development and homeostasis is unimpaired in ASNS-deficient mice (A) Western blots show levels of ASNS protein in lysates of OT-1 T cells stimulated in IMDM with SIINFEKL peptide ± rapamycin (rap), for the indicated time periods, or thymocytes. (B) ASNS levels in lysates from activated lymph node T cell from homozygous, heterozygous or wild-type (WT) Asns Tm1a mice. For (A, B) β actin serves as a protein loading control. Values in bar graph represent relative ASNS expression levels (ASNS/Actin) calculated using the LI-COR Odyssey imaging system from 1 of 2 repeated experiments. (C) Thymocyte and lymph node (LN) cell populations from littermate Asns Tm1a mice were analysed by flow cytometry. Total thymocytes (D) and LN CD4 and CD8 T cells (E) were enumerated from 3-4 age-matched male mice of each genotype. (F) Proportions of naïve, effector and memory cells within gated CD4 + and CD8 + T cell populations were analysed by analysis of CD44 and CD62L expression by flow cytometry. FACS dotplots are representative of 5-6 mice of each genotype. Values on dotplots represent % cells within the defined gates. NS – not significant, as determined by one-way ANOVA.

    Article Snippet: Primary antibodies (rabbit anti-ASNS – HPA029318, Atlas Antibodies, mouse anti-β actin, Clone AC-15, Sigma Aldrich) were diluted in LI-COR blocking buffer and detected using goat anti-rabbit-AF680 and goat anti-mouse-AF790 secondary reagents (Molecular Probes).

    Techniques: Mouse Assay, Western Blot, Expressing, Imaging, Flow Cytometry, FACS

    Immunoblot analysis of the expression and phosphorylation status of pro-survival and pro-apoptotic proteins. ( a ) Lysates prepared from helped or unhelped CTLs obtained from WT or CD4 + T cell-deficient mice were subjected to SDS-PAGE, and transferred to the nitrocellulose membrane. Western blotting was performed with a panel of Abs specific for β-actin, Bcl-2, Bcl10, Akt1, NF-κB-p65, phosphorylated-Akt1 and -NF-kB-p65, cleaved Caspases-3 and -7, or NFATc1 transcription factor and analyzed by the ODYSSEY densitometer. Densitometric values were normalized on the β-actin control and n-fold changes of normalized targets in the helped CTLs of WT or Ia b-/- mice relative to the unhelped CTLs in Ia b-/- mice are shown below the corresponding lanes. Data are derived from samples pooled from four to six mice in each group of the first experiment. The results of the second experiment are consistent with the first experiment (data not shown). One representative of two independent experiments is shown. ( b ) Representative FACS plots of helped CTLs from WT or CD4-deficient mice, or unhelped CTLs from CD4-deficient mice. Blood samples from helped CTLs in WT and Ia b-/- mice and unhelped CTL in Ia b-/- mice were collected 16 days of adoptive transfer and processed for triple staining. Value in panel a, b and c represents percentage of tetramer + -and CD8 + -specific double-positive cells in the total of CD8 + T cell population. The tetramer + -and CD8 + -specific double-positive cell populations were gated (in the circle) for analysis of TRAIL expression. Histogram overlays (right panels) of CD8 + tetramer + -gated helped CTLs from WT (histogram green filled overlays; right top panel) or CD4-deficient mice (histogram red filled overlays; right bottom panel) and CD8 + tetramer + -gated unhelped CTLs from CD4-deficient mice (histogram grey filled overlays; right top and bottom panel). The mean of fluorescence intensity (MFI) was calculated from triplicate values. The value in each panel represents the mean of MFI ± SD of positive staining cells (grey) versus the controls with MFI of 0.5 for green and MFI of 0.8 for red, respectively. One representative of two independent experiments is shown.

    Journal: PLoS ONE

    Article Title: Th Cells Promote CTL Survival and Memory via Acquired pMHC-I and Endogenous IL-2 and CD40L Signaling and by Modulating Apoptosis-Controlling Pathways

    doi: 10.1371/journal.pone.0064787

    Figure Lengend Snippet: Immunoblot analysis of the expression and phosphorylation status of pro-survival and pro-apoptotic proteins. ( a ) Lysates prepared from helped or unhelped CTLs obtained from WT or CD4 + T cell-deficient mice were subjected to SDS-PAGE, and transferred to the nitrocellulose membrane. Western blotting was performed with a panel of Abs specific for β-actin, Bcl-2, Bcl10, Akt1, NF-κB-p65, phosphorylated-Akt1 and -NF-kB-p65, cleaved Caspases-3 and -7, or NFATc1 transcription factor and analyzed by the ODYSSEY densitometer. Densitometric values were normalized on the β-actin control and n-fold changes of normalized targets in the helped CTLs of WT or Ia b-/- mice relative to the unhelped CTLs in Ia b-/- mice are shown below the corresponding lanes. Data are derived from samples pooled from four to six mice in each group of the first experiment. The results of the second experiment are consistent with the first experiment (data not shown). One representative of two independent experiments is shown. ( b ) Representative FACS plots of helped CTLs from WT or CD4-deficient mice, or unhelped CTLs from CD4-deficient mice. Blood samples from helped CTLs in WT and Ia b-/- mice and unhelped CTL in Ia b-/- mice were collected 16 days of adoptive transfer and processed for triple staining. Value in panel a, b and c represents percentage of tetramer + -and CD8 + -specific double-positive cells in the total of CD8 + T cell population. The tetramer + -and CD8 + -specific double-positive cell populations were gated (in the circle) for analysis of TRAIL expression. Histogram overlays (right panels) of CD8 + tetramer + -gated helped CTLs from WT (histogram green filled overlays; right top panel) or CD4-deficient mice (histogram red filled overlays; right bottom panel) and CD8 + tetramer + -gated unhelped CTLs from CD4-deficient mice (histogram grey filled overlays; right top and bottom panel). The mean of fluorescence intensity (MFI) was calculated from triplicate values. The value in each panel represents the mean of MFI ± SD of positive staining cells (grey) versus the controls with MFI of 0.5 for green and MFI of 0.8 for red, respectively. One representative of two independent experiments is shown.

    Article Snippet: The blots were incubated with goat anti-rabbit IRDyeR800/680CW, and band densities were quantified using ODYSSEY densitometer (LI-COR Bioscience).

    Techniques: Expressing, Mouse Assay, SDS Page, Western Blot, IA, Derivative Assay, FACS, CTL Assay, Adoptive Transfer Assay, Staining, Fluorescence

    In vitro (A – C) and in vivo (D and E) validation of mINDY-specific siRNA (siINDY) . (A) Dose–response curves upon transfection of HEK293 cells stably overexpressing mINDY carrying a FLAG-tag (HEK293-mINDY) with siINDY and its unmodified form (siINDY*) (n = 2). (B) Transfection of HEK293-mINDY with siINDY reduced mINDY protein in a concentration-dependent manner, determined by anti-FLAG In-Cell Western Assay (n = 3). The black arrow indicates background fluorescence signal of HEK293 wild-type cells analyzed in parallel. (C) Transfection of HEK293-mINDY cells with siINDY reduced cellular citrate uptake (n = 3). (D) Pilot mouse study to evaluate the siRNA efficacy. mINDY mRNA expression in liver 2 and 6 days after injection normalized to GAPDH and control siRNA at day 2 (n = 3). (E) mINDY mRNA expression in liver, kidney, and muscle tissue after 8 weeks of siRNA treatment normalized to GAPDH and control siRNA in liver (n = 4). Data are represented as mean ± SEM. Two-way ANOVA with Bonferroni's multiple comparisons test. Scrambled control siRNA (SCR): open bars; off-target-specific mINDY-unrelated siRNA (OT): fasciated bars; mINDY-specific unmodified siRNA (siINDY*): grey triangle; mINDY-specific modified siRNA (siINDY): black bars.

    Journal: Molecular Metabolism

    Article Title: Inhibition of citrate cotransporter Slc13a5/mINDY by RNAi improves hepatic insulin sensitivity and prevents diet-induced non-alcoholic fatty liver disease in mice

    doi: 10.1016/j.molmet.2016.08.004

    Figure Lengend Snippet: In vitro (A – C) and in vivo (D and E) validation of mINDY-specific siRNA (siINDY) . (A) Dose–response curves upon transfection of HEK293 cells stably overexpressing mINDY carrying a FLAG-tag (HEK293-mINDY) with siINDY and its unmodified form (siINDY*) (n = 2). (B) Transfection of HEK293-mINDY with siINDY reduced mINDY protein in a concentration-dependent manner, determined by anti-FLAG In-Cell Western Assay (n = 3). The black arrow indicates background fluorescence signal of HEK293 wild-type cells analyzed in parallel. (C) Transfection of HEK293-mINDY cells with siINDY reduced cellular citrate uptake (n = 3). (D) Pilot mouse study to evaluate the siRNA efficacy. mINDY mRNA expression in liver 2 and 6 days after injection normalized to GAPDH and control siRNA at day 2 (n = 3). (E) mINDY mRNA expression in liver, kidney, and muscle tissue after 8 weeks of siRNA treatment normalized to GAPDH and control siRNA in liver (n = 4). Data are represented as mean ± SEM. Two-way ANOVA with Bonferroni's multiple comparisons test. Scrambled control siRNA (SCR): open bars; off-target-specific mINDY-unrelated siRNA (OT): fasciated bars; mINDY-specific unmodified siRNA (siINDY*): grey triangle; mINDY-specific modified siRNA (siINDY): black bars.

    Article Snippet: 48 h post-transfection, cells were fixed with 3.7% formaldehyde and the Odyssey In-Cell Western Assay (LiCor, Bad Homburg, Germany) was performed according to the manufacturer's instructions using an anti-FLAG M2 antibody (Sigma) overnight for detection of FLAG-tagged mINDY.

    Techniques: In Vitro, In Vivo, Transfection, Stable Transfection, FLAG-tag, Concentration Assay, In-Cell ELISA, Fluorescence, Expressing, Injection, Modification