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Addgene inc oct4 tdtomato donor plasmid
Small molecules significantly promote CRISPR-Cpf1-mediated generation of knockin hPSC lines without drug selection. a A scheme of the targeting strategy of <t>OCT4-2A-tdTomato.</t> b FACS analysis for OCT4-tdTomato-positive cells after transfection of the OCT4-tdTomato donor plasmid and the OCT4-targeting crRNA into hPSCs. A representative FACS result of samples treated with DMSO, VE-822, and AZD-7762 was shown. c Addition of VE-822 and AZD-7762 increased the percentage of OCT4-tdTomato-positive cells. n = 3 experiments. Statistical significance calculated using two-tailed Student’s t -test, compared to DMSO controls. * P
Oct4 Tdtomato Donor Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oct4 tdtomato donor plasmid/product/Addgene inc
Average 82 stars, based on 1 article reviews
Price from $9.99 to $1999.99
oct4 tdtomato donor plasmid - by Bioz Stars, 2020-01
82/100 stars

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1) Product Images from "Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells"

Article Title: Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells

Journal: Nature Communications

doi: 10.1038/s41467-018-03760-5

Small molecules significantly promote CRISPR-Cpf1-mediated generation of knockin hPSC lines without drug selection. a A scheme of the targeting strategy of OCT4-2A-tdTomato. b FACS analysis for OCT4-tdTomato-positive cells after transfection of the OCT4-tdTomato donor plasmid and the OCT4-targeting crRNA into hPSCs. A representative FACS result of samples treated with DMSO, VE-822, and AZD-7762 was shown. c Addition of VE-822 and AZD-7762 increased the percentage of OCT4-tdTomato-positive cells. n = 3 experiments. Statistical significance calculated using two-tailed Student’s t -test, compared to DMSO controls. * P
Figure Legend Snippet: Small molecules significantly promote CRISPR-Cpf1-mediated generation of knockin hPSC lines without drug selection. a A scheme of the targeting strategy of OCT4-2A-tdTomato. b FACS analysis for OCT4-tdTomato-positive cells after transfection of the OCT4-tdTomato donor plasmid and the OCT4-targeting crRNA into hPSCs. A representative FACS result of samples treated with DMSO, VE-822, and AZD-7762 was shown. c Addition of VE-822 and AZD-7762 increased the percentage of OCT4-tdTomato-positive cells. n = 3 experiments. Statistical significance calculated using two-tailed Student’s t -test, compared to DMSO controls. * P

Techniques Used: CRISPR, Knock-In, Selection, FACS, Transfection, Plasmid Preparation, Two Tailed Test

Related Articles

Clone Assay:

Article Title: Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells
Article Snippet: For pCpfcr-crRNA vectors expressing Cpf1 target sequence, a 24-bp oligo located 3′ end of the PAM sequence was designed, annealed, and cloned into BbsI-digested pCpfcr. .. For OCT4-tdTomato donor plasmid, OCT4-2A-mOrange donor plasmid (Addgene, Plasmid #66986) was PCR amplified without mOrange sequence as the backbone, and the tdTomato sequence was amplified and linked with backbone by using Gibson Assembly kit (New England Biolabs).

Amplification:

Article Title: Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells
Article Snippet: For pCpfcr-crRNA vectors expressing Cpf1 target sequence, a 24-bp oligo located 3′ end of the PAM sequence was designed, annealed, and cloned into BbsI-digested pCpfcr. .. For OCT4-tdTomato donor plasmid, OCT4-2A-mOrange donor plasmid (Addgene, Plasmid #66986) was PCR amplified without mOrange sequence as the backbone, and the tdTomato sequence was amplified and linked with backbone by using Gibson Assembly kit (New England Biolabs). .. All vectors were checked by Sanger sequencing.

Polymerase Chain Reaction:

Article Title: Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells
Article Snippet: For pCpfcr-crRNA vectors expressing Cpf1 target sequence, a 24-bp oligo located 3′ end of the PAM sequence was designed, annealed, and cloned into BbsI-digested pCpfcr. .. For OCT4-tdTomato donor plasmid, OCT4-2A-mOrange donor plasmid (Addgene, Plasmid #66986) was PCR amplified without mOrange sequence as the backbone, and the tdTomato sequence was amplified and linked with backbone by using Gibson Assembly kit (New England Biolabs). .. All vectors were checked by Sanger sequencing.

Expressing:

Article Title: Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells
Article Snippet: For pCpfcr-crRNA vectors expressing Cpf1 target sequence, a 24-bp oligo located 3′ end of the PAM sequence was designed, annealed, and cloned into BbsI-digested pCpfcr. .. For OCT4-tdTomato donor plasmid, OCT4-2A-mOrange donor plasmid (Addgene, Plasmid #66986) was PCR amplified without mOrange sequence as the backbone, and the tdTomato sequence was amplified and linked with backbone by using Gibson Assembly kit (New England Biolabs).

Sequencing:

Article Title: Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells
Article Snippet: For pCpfcr-crRNA vectors expressing Cpf1 target sequence, a 24-bp oligo located 3′ end of the PAM sequence was designed, annealed, and cloned into BbsI-digested pCpfcr. .. For OCT4-tdTomato donor plasmid, OCT4-2A-mOrange donor plasmid (Addgene, Plasmid #66986) was PCR amplified without mOrange sequence as the backbone, and the tdTomato sequence was amplified and linked with backbone by using Gibson Assembly kit (New England Biolabs). .. All vectors were checked by Sanger sequencing.

Plasmid Preparation:

Article Title: Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells
Article Snippet: For pCpfcr-crRNA vectors expressing Cpf1 target sequence, a 24-bp oligo located 3′ end of the PAM sequence was designed, annealed, and cloned into BbsI-digested pCpfcr. .. For OCT4-tdTomato donor plasmid, OCT4-2A-mOrange donor plasmid (Addgene, Plasmid #66986) was PCR amplified without mOrange sequence as the backbone, and the tdTomato sequence was amplified and linked with backbone by using Gibson Assembly kit (New England Biolabs). .. All vectors were checked by Sanger sequencing.

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    Addgene inc oct4 tdtomato donor plasmid
    Small molecules significantly promote CRISPR-Cpf1-mediated generation of knockin hPSC lines without drug selection. a A scheme of the targeting strategy of <t>OCT4-2A-tdTomato.</t> b FACS analysis for OCT4-tdTomato-positive cells after transfection of the OCT4-tdTomato donor plasmid and the OCT4-targeting crRNA into hPSCs. A representative FACS result of samples treated with DMSO, VE-822, and AZD-7762 was shown. c Addition of VE-822 and AZD-7762 increased the percentage of OCT4-tdTomato-positive cells. n = 3 experiments. Statistical significance calculated using two-tailed Student’s t -test, compared to DMSO controls. * P
    Oct4 Tdtomato Donor Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oct4 tdtomato donor plasmid/product/Addgene inc
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    oct4 tdtomato donor plasmid - by Bioz Stars, 2020-01
    82/100 stars
      Buy from Supplier

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    Small molecules significantly promote CRISPR-Cpf1-mediated generation of knockin hPSC lines without drug selection. a A scheme of the targeting strategy of OCT4-2A-tdTomato. b FACS analysis for OCT4-tdTomato-positive cells after transfection of the OCT4-tdTomato donor plasmid and the OCT4-targeting crRNA into hPSCs. A representative FACS result of samples treated with DMSO, VE-822, and AZD-7762 was shown. c Addition of VE-822 and AZD-7762 increased the percentage of OCT4-tdTomato-positive cells. n = 3 experiments. Statistical significance calculated using two-tailed Student’s t -test, compared to DMSO controls. * P

    Journal: Nature Communications

    Article Title: Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells

    doi: 10.1038/s41467-018-03760-5

    Figure Lengend Snippet: Small molecules significantly promote CRISPR-Cpf1-mediated generation of knockin hPSC lines without drug selection. a A scheme of the targeting strategy of OCT4-2A-tdTomato. b FACS analysis for OCT4-tdTomato-positive cells after transfection of the OCT4-tdTomato donor plasmid and the OCT4-targeting crRNA into hPSCs. A representative FACS result of samples treated with DMSO, VE-822, and AZD-7762 was shown. c Addition of VE-822 and AZD-7762 increased the percentage of OCT4-tdTomato-positive cells. n = 3 experiments. Statistical significance calculated using two-tailed Student’s t -test, compared to DMSO controls. * P

    Article Snippet: For OCT4-tdTomato donor plasmid, OCT4-2A-mOrange donor plasmid (Addgene, Plasmid #66986) was PCR amplified without mOrange sequence as the backbone, and the tdTomato sequence was amplified and linked with backbone by using Gibson Assembly kit (New England Biolabs).

    Techniques: CRISPR, Knock-In, Selection, FACS, Transfection, Plasmid Preparation, Two Tailed Test