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oroboros o2k fluorometer  (Oroboros Instruments)
86
Oroboros Instruments oroboros o2k fluorometer
(A) Mitochondrial respiration and H 2 O 2 production were measured using an Oroboros <t>O2k</t> fluorometer in mitochondria isolated from WT and HuR Hep−/− livers under untreated (control) conditions and at 2 h after APAP treatment (2 h APAP). Oxygen consumption was assessed sequentially following the addition of malate, ADP, succinate, and FCCP. n = 3–4 mice per group. (B) Mitochondrial redox status, expressed as basal H 2 O 2 production normalized to basal O 2 flux, and OXPHOS capacity, calculated as State 3 respiration minus leak respiration. n = 3–4 mice per group. (C) Representative transmission electron micrographs (TEMs) of WT and HuR Hep−/− mouse livers collected under control conditions and at indicated time points following APAP treatment. TEM analysis was performed using one mouse per genotype and time point. Mitochondrial features were quantified at the organelle level and are presented as within-animal distributions. Mitochondrial number represents the total number of mitochondria per micrograph, whereas mitochondrial area represents the total surface area occupied by mitochondria in each micrograph. (D) Western blot analysis of mitochondrial dynamics proteins Opa1, Mfn2, and Drp1 in livers collected 2 h after APAP treatment. Protein levels were normalized to GAPDH. Data are represented as mean ± SEM. n = 5–6 mice per group. Each data point represents an individual mouse. Statistical differences between 2 groups were analyzed using an unpaired 2-tailed Student’s t test. Comparisons involving multiple genotypes and time points were analyzed using two-way ANOVA followed by Sidak’s multiple comparisons test. * p < 0.05, ** p < 0.01. Abbreviations: ADP, adenine diphosphate; FCCP, carbonyl cyanide-p-(trifluoromethyoxy) phenylhydrazone; M, mitochondria; N, nucleus, Opa1, optic atrophy 1; Mfn2, mitofusion 2; Drp1, dynamin-related protein 1.
Oroboros O2k Fluorometer, supplied by Oroboros Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/o2k+fluorometer/high+resolution+respirometry/pmc13202677-106-13-16
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oroboros o2k fluorometer - by Bioz Stars, 2026-11
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o2k fluorometer  (Oroboros Instruments)
86
Oroboros Instruments o2k fluorometer
(A) Mitochondrial respiration and H 2 O 2 production were measured using an Oroboros <t>O2k</t> fluorometer in mitochondria isolated from WT and HuR Hep−/− livers under untreated (control) conditions and at 2 h after APAP treatment (2 h APAP). Oxygen consumption was assessed sequentially following the addition of malate, ADP, succinate, and FCCP. n = 3–4 mice per group. (B) Mitochondrial redox status, expressed as basal H 2 O 2 production normalized to basal O 2 flux, and OXPHOS capacity, calculated as State 3 respiration minus leak respiration. n = 3–4 mice per group. (C) Representative transmission electron micrographs (TEMs) of WT and HuR Hep−/− mouse livers collected under control conditions and at indicated time points following APAP treatment. TEM analysis was performed using one mouse per genotype and time point. Mitochondrial features were quantified at the organelle level and are presented as within-animal distributions. Mitochondrial number represents the total number of mitochondria per micrograph, whereas mitochondrial area represents the total surface area occupied by mitochondria in each micrograph. (D) Western blot analysis of mitochondrial dynamics proteins Opa1, Mfn2, and Drp1 in livers collected 2 h after APAP treatment. Protein levels were normalized to GAPDH. Data are represented as mean ± SEM. n = 5–6 mice per group. Each data point represents an individual mouse. Statistical differences between 2 groups were analyzed using an unpaired 2-tailed Student’s t test. Comparisons involving multiple genotypes and time points were analyzed using two-way ANOVA followed by Sidak’s multiple comparisons test. * p < 0.05, ** p < 0.01. Abbreviations: ADP, adenine diphosphate; FCCP, carbonyl cyanide-p-(trifluoromethyoxy) phenylhydrazone; M, mitochondria; N, nucleus, Opa1, optic atrophy 1; Mfn2, mitofusion 2; Drp1, dynamin-related protein 1.
O2k Fluorometer, supplied by Oroboros Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/o2k+fluorometer/high+resolution+respirometry/pmc12561179-107-27-28
Average 86 stars, based on 1 article reviews
o2k fluorometer - by Bioz Stars, 2026-11
86/100 stars
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o2k-fluorometer  (Oroboros Instruments)
90
Oroboros Instruments o2k-fluorometer
(A) Mitochondrial respiration and H 2 O 2 production were measured using an Oroboros <t>O2k</t> fluorometer in mitochondria isolated from WT and HuR Hep−/− livers under untreated (control) conditions and at 2 h after APAP treatment (2 h APAP). Oxygen consumption was assessed sequentially following the addition of malate, ADP, succinate, and FCCP. n = 3–4 mice per group. (B) Mitochondrial redox status, expressed as basal H 2 O 2 production normalized to basal O 2 flux, and OXPHOS capacity, calculated as State 3 respiration minus leak respiration. n = 3–4 mice per group. (C) Representative transmission electron micrographs (TEMs) of WT and HuR Hep−/− mouse livers collected under control conditions and at indicated time points following APAP treatment. TEM analysis was performed using one mouse per genotype and time point. Mitochondrial features were quantified at the organelle level and are presented as within-animal distributions. Mitochondrial number represents the total number of mitochondria per micrograph, whereas mitochondrial area represents the total surface area occupied by mitochondria in each micrograph. (D) Western blot analysis of mitochondrial dynamics proteins Opa1, Mfn2, and Drp1 in livers collected 2 h after APAP treatment. Protein levels were normalized to GAPDH. Data are represented as mean ± SEM. n = 5–6 mice per group. Each data point represents an individual mouse. Statistical differences between 2 groups were analyzed using an unpaired 2-tailed Student’s t test. Comparisons involving multiple genotypes and time points were analyzed using two-way ANOVA followed by Sidak’s multiple comparisons test. * p < 0.05, ** p < 0.01. Abbreviations: ADP, adenine diphosphate; FCCP, carbonyl cyanide-p-(trifluoromethyoxy) phenylhydrazone; M, mitochondria; N, nucleus, Opa1, optic atrophy 1; Mfn2, mitofusion 2; Drp1, dynamin-related protein 1.
O2k Fluorometer, supplied by Oroboros Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/o2k+fluorometer/oxygraph+2k/pm40542676-76-10-10
Average 90 stars, based on 1 article reviews
o2k-fluorometer - by Bioz Stars, 2026-11
90/100 stars
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orobos o2k-fluorometer  (Oroboros Instruments)
90
Oroboros Instruments orobos o2k-fluorometer
(A) Mitochondrial respiration and H 2 O 2 production were measured using an Oroboros <t>O2k</t> fluorometer in mitochondria isolated from WT and HuR Hep−/− livers under untreated (control) conditions and at 2 h after APAP treatment (2 h APAP). Oxygen consumption was assessed sequentially following the addition of malate, ADP, succinate, and FCCP. n = 3–4 mice per group. (B) Mitochondrial redox status, expressed as basal H 2 O 2 production normalized to basal O 2 flux, and OXPHOS capacity, calculated as State 3 respiration minus leak respiration. n = 3–4 mice per group. (C) Representative transmission electron micrographs (TEMs) of WT and HuR Hep−/− mouse livers collected under control conditions and at indicated time points following APAP treatment. TEM analysis was performed using one mouse per genotype and time point. Mitochondrial features were quantified at the organelle level and are presented as within-animal distributions. Mitochondrial number represents the total number of mitochondria per micrograph, whereas mitochondrial area represents the total surface area occupied by mitochondria in each micrograph. (D) Western blot analysis of mitochondrial dynamics proteins Opa1, Mfn2, and Drp1 in livers collected 2 h after APAP treatment. Protein levels were normalized to GAPDH. Data are represented as mean ± SEM. n = 5–6 mice per group. Each data point represents an individual mouse. Statistical differences between 2 groups were analyzed using an unpaired 2-tailed Student’s t test. Comparisons involving multiple genotypes and time points were analyzed using two-way ANOVA followed by Sidak’s multiple comparisons test. * p < 0.05, ** p < 0.01. Abbreviations: ADP, adenine diphosphate; FCCP, carbonyl cyanide-p-(trifluoromethyoxy) phenylhydrazone; M, mitochondria; N, nucleus, Opa1, optic atrophy 1; Mfn2, mitofusion 2; Drp1, dynamin-related protein 1.
Orobos O2k Fluorometer, supplied by Oroboros Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/o2k+fluorometer/oxygraph+2k/pmc12148014-73-26-28
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orobos o2k-fluorometer - by Bioz Stars, 2026-11
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o2k-fluorometer module  (Oroboros Instruments)
90
Oroboros Instruments o2k-fluorometer module
(A) Mitochondrial respiration and H 2 O 2 production were measured using an Oroboros <t>O2k</t> fluorometer in mitochondria isolated from WT and HuR Hep−/− livers under untreated (control) conditions and at 2 h after APAP treatment (2 h APAP). Oxygen consumption was assessed sequentially following the addition of malate, ADP, succinate, and FCCP. n = 3–4 mice per group. (B) Mitochondrial redox status, expressed as basal H 2 O 2 production normalized to basal O 2 flux, and OXPHOS capacity, calculated as State 3 respiration minus leak respiration. n = 3–4 mice per group. (C) Representative transmission electron micrographs (TEMs) of WT and HuR Hep−/− mouse livers collected under control conditions and at indicated time points following APAP treatment. TEM analysis was performed using one mouse per genotype and time point. Mitochondrial features were quantified at the organelle level and are presented as within-animal distributions. Mitochondrial number represents the total number of mitochondria per micrograph, whereas mitochondrial area represents the total surface area occupied by mitochondria in each micrograph. (D) Western blot analysis of mitochondrial dynamics proteins Opa1, Mfn2, and Drp1 in livers collected 2 h after APAP treatment. Protein levels were normalized to GAPDH. Data are represented as mean ± SEM. n = 5–6 mice per group. Each data point represents an individual mouse. Statistical differences between 2 groups were analyzed using an unpaired 2-tailed Student’s t test. Comparisons involving multiple genotypes and time points were analyzed using two-way ANOVA followed by Sidak’s multiple comparisons test. * p < 0.05, ** p < 0.01. Abbreviations: ADP, adenine diphosphate; FCCP, carbonyl cyanide-p-(trifluoromethyoxy) phenylhydrazone; M, mitochondria; N, nucleus, Opa1, optic atrophy 1; Mfn2, mitofusion 2; Drp1, dynamin-related protein 1.
O2k Fluorometer Module, supplied by Oroboros Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/o2k+fluorometer/o2k+fluorometer+module/pmc12162577-91-19-21
Average 90 stars, based on 1 article reviews
o2k-fluorometer module - by Bioz Stars, 2026-11
90/100 stars
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o2k-fluoromet r  (Oroboros Instruments)
90
Oroboros Instruments o2k-fluoromet r
(A) Mitochondrial respiration and H 2 O 2 production were measured using an Oroboros <t>O2k</t> fluorometer in mitochondria isolated from WT and HuR Hep−/− livers under untreated (control) conditions and at 2 h after APAP treatment (2 h APAP). Oxygen consumption was assessed sequentially following the addition of malate, ADP, succinate, and FCCP. n = 3–4 mice per group. (B) Mitochondrial redox status, expressed as basal H 2 O 2 production normalized to basal O 2 flux, and OXPHOS capacity, calculated as State 3 respiration minus leak respiration. n = 3–4 mice per group. (C) Representative transmission electron micrographs (TEMs) of WT and HuR Hep−/− mouse livers collected under control conditions and at indicated time points following APAP treatment. TEM analysis was performed using one mouse per genotype and time point. Mitochondrial features were quantified at the organelle level and are presented as within-animal distributions. Mitochondrial number represents the total number of mitochondria per micrograph, whereas mitochondrial area represents the total surface area occupied by mitochondria in each micrograph. (D) Western blot analysis of mitochondrial dynamics proteins Opa1, Mfn2, and Drp1 in livers collected 2 h after APAP treatment. Protein levels were normalized to GAPDH. Data are represented as mean ± SEM. n = 5–6 mice per group. Each data point represents an individual mouse. Statistical differences between 2 groups were analyzed using an unpaired 2-tailed Student’s t test. Comparisons involving multiple genotypes and time points were analyzed using two-way ANOVA followed by Sidak’s multiple comparisons test. * p < 0.05, ** p < 0.01. Abbreviations: ADP, adenine diphosphate; FCCP, carbonyl cyanide-p-(trifluoromethyoxy) phenylhydrazone; M, mitochondria; N, nucleus, Opa1, optic atrophy 1; Mfn2, mitofusion 2; Drp1, dynamin-related protein 1.
O2k Fluoromet R, supplied by Oroboros Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/o2k+fluorometer/o2k+fluoromet+r/pm40227392-314-25-27
Average 90 stars, based on 1 article reviews
o2k-fluoromet r - by Bioz Stars, 2026-11
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(A) Mitochondrial respiration and H 2 O 2 production were measured using an Oroboros O2k fluorometer in mitochondria isolated from WT and HuR Hep−/− livers under untreated (control) conditions and at 2 h after APAP treatment (2 h APAP). Oxygen consumption was assessed sequentially following the addition of malate, ADP, succinate, and FCCP. n = 3–4 mice per group. (B) Mitochondrial redox status, expressed as basal H 2 O 2 production normalized to basal O 2 flux, and OXPHOS capacity, calculated as State 3 respiration minus leak respiration. n = 3–4 mice per group. (C) Representative transmission electron micrographs (TEMs) of WT and HuR Hep−/− mouse livers collected under control conditions and at indicated time points following APAP treatment. TEM analysis was performed using one mouse per genotype and time point. Mitochondrial features were quantified at the organelle level and are presented as within-animal distributions. Mitochondrial number represents the total number of mitochondria per micrograph, whereas mitochondrial area represents the total surface area occupied by mitochondria in each micrograph. (D) Western blot analysis of mitochondrial dynamics proteins Opa1, Mfn2, and Drp1 in livers collected 2 h after APAP treatment. Protein levels were normalized to GAPDH. Data are represented as mean ± SEM. n = 5–6 mice per group. Each data point represents an individual mouse. Statistical differences between 2 groups were analyzed using an unpaired 2-tailed Student’s t test. Comparisons involving multiple genotypes and time points were analyzed using two-way ANOVA followed by Sidak’s multiple comparisons test. * p < 0.05, ** p < 0.01. Abbreviations: ADP, adenine diphosphate; FCCP, carbonyl cyanide-p-(trifluoromethyoxy) phenylhydrazone; M, mitochondria; N, nucleus, Opa1, optic atrophy 1; Mfn2, mitofusion 2; Drp1, dynamin-related protein 1.

Journal: Toxicology

Article Title: Dysregulation of xenobiotic metabolism and mitochondrial dysfunction exacerbate acetaminophen-induced hepatotoxicity in human antigen R-deficient male mice

doi: 10.1016/j.tox.2026.154480

Figure Lengend Snippet: (A) Mitochondrial respiration and H 2 O 2 production were measured using an Oroboros O2k fluorometer in mitochondria isolated from WT and HuR Hep−/− livers under untreated (control) conditions and at 2 h after APAP treatment (2 h APAP). Oxygen consumption was assessed sequentially following the addition of malate, ADP, succinate, and FCCP. n = 3–4 mice per group. (B) Mitochondrial redox status, expressed as basal H 2 O 2 production normalized to basal O 2 flux, and OXPHOS capacity, calculated as State 3 respiration minus leak respiration. n = 3–4 mice per group. (C) Representative transmission electron micrographs (TEMs) of WT and HuR Hep−/− mouse livers collected under control conditions and at indicated time points following APAP treatment. TEM analysis was performed using one mouse per genotype and time point. Mitochondrial features were quantified at the organelle level and are presented as within-animal distributions. Mitochondrial number represents the total number of mitochondria per micrograph, whereas mitochondrial area represents the total surface area occupied by mitochondria in each micrograph. (D) Western blot analysis of mitochondrial dynamics proteins Opa1, Mfn2, and Drp1 in livers collected 2 h after APAP treatment. Protein levels were normalized to GAPDH. Data are represented as mean ± SEM. n = 5–6 mice per group. Each data point represents an individual mouse. Statistical differences between 2 groups were analyzed using an unpaired 2-tailed Student’s t test. Comparisons involving multiple genotypes and time points were analyzed using two-way ANOVA followed by Sidak’s multiple comparisons test. * p < 0.05, ** p < 0.01. Abbreviations: ADP, adenine diphosphate; FCCP, carbonyl cyanide-p-(trifluoromethyoxy) phenylhydrazone; M, mitochondria; N, nucleus, Opa1, optic atrophy 1; Mfn2, mitofusion 2; Drp1, dynamin-related protein 1.

Article Snippet: Mitochondrial respiration and H 2 O 2 emission were measured simultaneously using an Oroboros O2K fluorometer (Oroboros Instruments, Austria) following established protocols ( ; ).

Techniques: Isolation, Control, Transmission Assay, Western Blot