o methyl serine dodecylamide hydrochloride msdh filipin staining  (Cayman Chemical)

 
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    Cayman Chemical o methyl serine dodecylamide hydrochloride msdh filipin staining
    Long term HPβCD treatment increases cellular cholesterol. ( Top Row ) - <t>Filipin</t> staining shows HPβCD treatment, LF loading and the two combined each cause substantial cellular cholesterol increase relative to healthy cells. ( Bottom Row ) Cells with increased lysosome cholesterol composition are more resistant to apoptosis induced by the lysomotropic agent <t>MSDH.</t> Healthy confluent cells were killed by MSDH exposure (45 uM MSDH for 42 hours) while HPβCD treated cells and LF loaded cells survived with no signs of visual stress. Therefore, HPβCD and LF modulate lysosome membrane cholesterol composition in a manner consistent with that suggested by filipin staining.
    O Methyl Serine Dodecylamide Hydrochloride Msdh Filipin Staining, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/o methyl serine dodecylamide hydrochloride msdh filipin staining/product/Cayman Chemical
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    o methyl serine dodecylamide hydrochloride msdh filipin staining - by Bioz Stars, 2020-05
    91/100 stars

    Related Products / Commonly Used Together

    filipin
    cholesterol cell-based detection assay kit

    Images

    1) Product Images from "2-Hydroxypropyl-beta-cyclodextrin (HPβCD) reduces age-related lipofuscin accumulation through a cholesterol-associated pathway"

    Article Title: 2-Hydroxypropyl-beta-cyclodextrin (HPβCD) reduces age-related lipofuscin accumulation through a cholesterol-associated pathway

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-02387-8

    Long term HPβCD treatment increases cellular cholesterol. ( Top Row ) - Filipin staining shows HPβCD treatment, LF loading and the two combined each cause substantial cellular cholesterol increase relative to healthy cells. ( Bottom Row ) Cells with increased lysosome cholesterol composition are more resistant to apoptosis induced by the lysomotropic agent MSDH. Healthy confluent cells were killed by MSDH exposure (45 uM MSDH for 42 hours) while HPβCD treated cells and LF loaded cells survived with no signs of visual stress. Therefore, HPβCD and LF modulate lysosome membrane cholesterol composition in a manner consistent with that suggested by filipin staining.
    Figure Legend Snippet: Long term HPβCD treatment increases cellular cholesterol. ( Top Row ) - Filipin staining shows HPβCD treatment, LF loading and the two combined each cause substantial cellular cholesterol increase relative to healthy cells. ( Bottom Row ) Cells with increased lysosome cholesterol composition are more resistant to apoptosis induced by the lysomotropic agent MSDH. Healthy confluent cells were killed by MSDH exposure (45 uM MSDH for 42 hours) while HPβCD treated cells and LF loaded cells survived with no signs of visual stress. Therefore, HPβCD and LF modulate lysosome membrane cholesterol composition in a manner consistent with that suggested by filipin staining.

    Techniques Used: Staining

    Related Articles

    Staining:

    Article Title: 2-Hydroxypropyl-beta-cyclodextrin (HPβCD) reduces age-related lipofuscin accumulation through a cholesterol-associated pathway
    Article Snippet: .. Cholesterol detection with filipin and O-methyl-serine dodecylamide hydrochloride (MSDH) Filipin staining was performed using the Cholesterol Cell-Based Detection Assay Kit (Cayman Chemical 10009779) and protocol. ..

    Detection Assay:

    Article Title: 2-Hydroxypropyl-beta-cyclodextrin (HPβCD) reduces age-related lipofuscin accumulation through a cholesterol-associated pathway
    Article Snippet: .. Cholesterol detection with filipin and O-methyl-serine dodecylamide hydrochloride (MSDH) Filipin staining was performed using the Cholesterol Cell-Based Detection Assay Kit (Cayman Chemical 10009779) and protocol. ..

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  • 91
    Cayman Chemical o methyl serine dodecylamide hydrochloride msdh filipin staining
    Long term HPβCD treatment increases cellular cholesterol. ( Top Row ) - <t>Filipin</t> staining shows HPβCD treatment, LF loading and the two combined each cause substantial cellular cholesterol increase relative to healthy cells. ( Bottom Row ) Cells with increased lysosome cholesterol composition are more resistant to apoptosis induced by the lysomotropic agent <t>MSDH.</t> Healthy confluent cells were killed by MSDH exposure (45 uM MSDH for 42 hours) while HPβCD treated cells and LF loaded cells survived with no signs of visual stress. Therefore, HPβCD and LF modulate lysosome membrane cholesterol composition in a manner consistent with that suggested by filipin staining.
    O Methyl Serine Dodecylamide Hydrochloride Msdh Filipin Staining, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/o methyl serine dodecylamide hydrochloride msdh filipin staining/product/Cayman Chemical
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    o methyl serine dodecylamide hydrochloride msdh filipin staining - by Bioz Stars, 2020-05
    91/100 stars
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    Long term HPβCD treatment increases cellular cholesterol. ( Top Row ) - Filipin staining shows HPβCD treatment, LF loading and the two combined each cause substantial cellular cholesterol increase relative to healthy cells. ( Bottom Row ) Cells with increased lysosome cholesterol composition are more resistant to apoptosis induced by the lysomotropic agent MSDH. Healthy confluent cells were killed by MSDH exposure (45 uM MSDH for 42 hours) while HPβCD treated cells and LF loaded cells survived with no signs of visual stress. Therefore, HPβCD and LF modulate lysosome membrane cholesterol composition in a manner consistent with that suggested by filipin staining.

    Journal: Scientific Reports

    Article Title: 2-Hydroxypropyl-beta-cyclodextrin (HPβCD) reduces age-related lipofuscin accumulation through a cholesterol-associated pathway

    doi: 10.1038/s41598-017-02387-8

    Figure Lengend Snippet: Long term HPβCD treatment increases cellular cholesterol. ( Top Row ) - Filipin staining shows HPβCD treatment, LF loading and the two combined each cause substantial cellular cholesterol increase relative to healthy cells. ( Bottom Row ) Cells with increased lysosome cholesterol composition are more resistant to apoptosis induced by the lysomotropic agent MSDH. Healthy confluent cells were killed by MSDH exposure (45 uM MSDH for 42 hours) while HPβCD treated cells and LF loaded cells survived with no signs of visual stress. Therefore, HPβCD and LF modulate lysosome membrane cholesterol composition in a manner consistent with that suggested by filipin staining.

    Article Snippet: Cholesterol detection with filipin and O-methyl-serine dodecylamide hydrochloride (MSDH) Filipin staining was performed using the Cholesterol Cell-Based Detection Assay Kit (Cayman Chemical 10009779) and protocol.

    Techniques: Staining