nupage mops sds running buffer  (Thermo Fisher)


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    Name:
    NuPAGE MOPS SDS Running Buffer 20X
    Description:
    NuPAGE MOPS SDS Running Buffer 20X is formulated for running proteins on NuPAGE Novex Bis Tris gels only NuPAGE MOPS SDS Running Buffer is recommended for separating medium to large sized proteins Use the right buffer to optimize protein separationsNuPAGE MES SDS Running Buffer and NuPAGE MOPS SDS Running Buffer can both be used with NuPAGE Novex Bis Tris gels Use of MOPS buffer allows proteins to run slower than when using MES buffer Get consistent convenient high quality resultsPre mixed NuPAGE buffers are a convenient way to ensure high quality consistent electrophoresis results All buffers are made with high purity reagents and are strictly quality controlled The buffers are provided as a concentrated solution and require simple dilution with deionized water before use Related links• Compare protein migration patterns with different combinations of gels and running buffers • Find NuPAGE Novex Bis Tris gels
    Catalog Number:
    np0001
    Price:
    None
    Applications:
    1D Gel Electrophoresis|NuPAGE® Novex® Bis-Tris Gel Electrophoresis|Protein Biology|Protein Gel Electrophoresis
    Category:
    Lab Reagents and Chemicals
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    Structured Review

    Thermo Fisher nupage mops sds running buffer
    <t>SDS-PAGE</t> of wt DevS642 (molecular weight 25330 Da) and H149A mutant after purification by affinity chromatography. In each lane about 0.5 μg of protein was loaded on a 12% BT Novex <t>NuPage</t> gel. Lane 1, SeeBlue Plus 2 prestained protein standard
    NuPAGE MOPS SDS Running Buffer 20X is formulated for running proteins on NuPAGE Novex Bis Tris gels only NuPAGE MOPS SDS Running Buffer is recommended for separating medium to large sized proteins Use the right buffer to optimize protein separationsNuPAGE MES SDS Running Buffer and NuPAGE MOPS SDS Running Buffer can both be used with NuPAGE Novex Bis Tris gels Use of MOPS buffer allows proteins to run slower than when using MES buffer Get consistent convenient high quality resultsPre mixed NuPAGE buffers are a convenient way to ensure high quality consistent electrophoresis results All buffers are made with high purity reagents and are strictly quality controlled The buffers are provided as a concentrated solution and require simple dilution with deionized water before use Related links• Compare protein migration patterns with different combinations of gels and running buffers • Find NuPAGE Novex Bis Tris gels
    https://www.bioz.com/result/nupage mops sds running buffer/product/Thermo Fisher
    Average 99 stars, based on 254 article reviews
    Price from $9.99 to $1999.99
    nupage mops sds running buffer - by Bioz Stars, 2020-10
    99/100 stars

    Images

    1) Product Images from "DevS, a Heme-Containing Two-Component Oxygen Sensor of Mycobacterium tuberculosis †"

    Article Title: DevS, a Heme-Containing Two-Component Oxygen Sensor of Mycobacterium tuberculosis †

    Journal:

    doi: 10.1021/bi602422p

    SDS-PAGE of wt DevS642 (molecular weight 25330 Da) and H149A mutant after purification by affinity chromatography. In each lane about 0.5 μg of protein was loaded on a 12% BT Novex NuPage gel. Lane 1, SeeBlue Plus 2 prestained protein standard
    Figure Legend Snippet: SDS-PAGE of wt DevS642 (molecular weight 25330 Da) and H149A mutant after purification by affinity chromatography. In each lane about 0.5 μg of protein was loaded on a 12% BT Novex NuPage gel. Lane 1, SeeBlue Plus 2 prestained protein standard

    Techniques Used: SDS Page, Molecular Weight, Mutagenesis, Purification, Affinity Chromatography

    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Stress‐induced modulation of volume‐regulated anions channels in human alveolar carcinoma cells . Stress‐induced modulation of volume‐regulated anions channels in human alveolar carcinoma cells
    Article Snippet: .. Protein samples were mixed with NuPAGE sample buffer containing dithiothreitol (DTT) and proceeded for SDS‐PAGE gel electrophoresis (NuPAGE precast 10% or 4–12% Bis‐tris gels in NuPAGE MOPS SDS running buffer, Invitrogen, Waltham, MA) in NOVEX chambers under reducing/denaturing conditions. .. To indicate the molecular weight of proteins we used a benchmark protein ladder (Invitrogen).

    Transfection:

    Article Title: Role of microRNAs in epigenetic silencing of the CHD5 tumor suppressor gene in neuroblastomas
    Article Snippet: .. Western analysis Whole cell extracts (100 μg), either transfected with indicated miRNAs or mock transfected, were subjected to polyacrylamide gel electrophoresis (4-12% SDS-PAGE), using NuPAGE Bis-Tris gels with MOPS-SDS Running Buffer (Invitrogen, Grand Island, NY). .. Allstars siRNA and miRNA-454 were used as negative controls.

    Purification:

    Article Title: Use of mariner transposases for one-step delivery and integration of DNA in prokaryotes and eukaryotes by transfection
    Article Snippet: .. Subsequently, 250 μg of total protein extracts or recombinant purified proteins (GFP: 1 ng per lane, GFP-Mos1: 10 ng per lane) were separated on 4–12% NuPAGE SDS-PAGE gels using MOPS running buffer (ThermoFisher) and were transferred onto a nitrocellulose membrane (GE Healthcare). .. The membrane was blocked overnight at 4°C with 1:10 Western Blocking Reagent (Roche) in Tris-buffered saline, Tween 20 (TBST) buffer (20 mM Tris pH 7.5, 137 mM NaCl and 0.1% (v/v) Tween 20).

    SDS-Gel:

    Article Title: Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine production
    Article Snippet: .. For molecular mass analysis post digestion, 2 μg of each protein was electrophoresed on a 4–12% Bis-Tris PAGE SDS gel in MOPS running buffer, and stained with Coomassie Simply Blue (Thermo Scientific, Waltham, MA). .. Supporting information Binding of V2 antibodies CH58 and CH59 to A244-rgp120 produced in normal and A244_N332-rgp120 produced in MGAT1- CHO cell lines.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine production
    Article Snippet: .. For molecular mass analysis post digestion, 2 μg of each protein was electrophoresed on a 4–12% Bis-Tris PAGE SDS gel in MOPS running buffer, and stained with Coomassie Simply Blue (Thermo Scientific, Waltham, MA). .. Supporting information Binding of V2 antibodies CH58 and CH59 to A244-rgp120 produced in normal and A244_N332-rgp120 produced in MGAT1- CHO cell lines.

    Article Title: Two p53 tetramers bind one consensus DNA response element
    Article Snippet: .. 1x MOPS SDS buffer (Invitrogen) was used for the denaturing PAGE. .. Anode (25 mM imidazole. pH 7) and cathode (50 mM tricine, 7.5mM imidazole, 0.002% Coomassie blue-G250. pH 7) buffers were used for the Native PAGE.

    Article Title: Role of microRNAs in epigenetic silencing of the CHD5 tumor suppressor gene in neuroblastomas
    Article Snippet: .. Western analysis Whole cell extracts (100 μg), either transfected with indicated miRNAs or mock transfected, were subjected to polyacrylamide gel electrophoresis (4-12% SDS-PAGE), using NuPAGE Bis-Tris gels with MOPS-SDS Running Buffer (Invitrogen, Grand Island, NY). .. Allstars siRNA and miRNA-454 were used as negative controls.

    Staining:

    Article Title: Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine production
    Article Snippet: .. For molecular mass analysis post digestion, 2 μg of each protein was electrophoresed on a 4–12% Bis-Tris PAGE SDS gel in MOPS running buffer, and stained with Coomassie Simply Blue (Thermo Scientific, Waltham, MA). .. Supporting information Binding of V2 antibodies CH58 and CH59 to A244-rgp120 produced in normal and A244_N332-rgp120 produced in MGAT1- CHO cell lines.

    Article Title: DevS, a Heme-Containing Two-Component Oxygen Sensor of Mycobacterium tuberculosis †
    Article Snippet: .. Simply Blue Safe Stain, In Vision Histag In-Gel Stain, See Blue Plus 2 protein markers, S.O.C. medium, along with the gel apparatus (Novex MiniCell), NuPage 12% Bis-Tris gels and NuPage MOPS SDS running buffer were purchased from Invitrogen. .. The E. coli XL-10 gold, BL21DE3, and XL-1 Blue cells were from Stratagene.

    Western Blot:

    Article Title: Role of microRNAs in epigenetic silencing of the CHD5 tumor suppressor gene in neuroblastomas
    Article Snippet: .. Western analysis Whole cell extracts (100 μg), either transfected with indicated miRNAs or mock transfected, were subjected to polyacrylamide gel electrophoresis (4-12% SDS-PAGE), using NuPAGE Bis-Tris gels with MOPS-SDS Running Buffer (Invitrogen, Grand Island, NY). .. Allstars siRNA and miRNA-454 were used as negative controls.

    Recombinant:

    Article Title: Use of mariner transposases for one-step delivery and integration of DNA in prokaryotes and eukaryotes by transfection
    Article Snippet: .. Subsequently, 250 μg of total protein extracts or recombinant purified proteins (GFP: 1 ng per lane, GFP-Mos1: 10 ng per lane) were separated on 4–12% NuPAGE SDS-PAGE gels using MOPS running buffer (ThermoFisher) and were transferred onto a nitrocellulose membrane (GE Healthcare). .. The membrane was blocked overnight at 4°C with 1:10 Western Blocking Reagent (Roche) in Tris-buffered saline, Tween 20 (TBST) buffer (20 mM Tris pH 7.5, 137 mM NaCl and 0.1% (v/v) Tween 20).

    SDS Page:

    Article Title: Stress‐induced modulation of volume‐regulated anions channels in human alveolar carcinoma cells . Stress‐induced modulation of volume‐regulated anions channels in human alveolar carcinoma cells
    Article Snippet: .. Protein samples were mixed with NuPAGE sample buffer containing dithiothreitol (DTT) and proceeded for SDS‐PAGE gel electrophoresis (NuPAGE precast 10% or 4–12% Bis‐tris gels in NuPAGE MOPS SDS running buffer, Invitrogen, Waltham, MA) in NOVEX chambers under reducing/denaturing conditions. .. To indicate the molecular weight of proteins we used a benchmark protein ladder (Invitrogen).

    Article Title: Role of microRNAs in epigenetic silencing of the CHD5 tumor suppressor gene in neuroblastomas
    Article Snippet: .. Western analysis Whole cell extracts (100 μg), either transfected with indicated miRNAs or mock transfected, were subjected to polyacrylamide gel electrophoresis (4-12% SDS-PAGE), using NuPAGE Bis-Tris gels with MOPS-SDS Running Buffer (Invitrogen, Grand Island, NY). .. Allstars siRNA and miRNA-454 were used as negative controls.

    Article Title: Use of mariner transposases for one-step delivery and integration of DNA in prokaryotes and eukaryotes by transfection
    Article Snippet: .. Subsequently, 250 μg of total protein extracts or recombinant purified proteins (GFP: 1 ng per lane, GFP-Mos1: 10 ng per lane) were separated on 4–12% NuPAGE SDS-PAGE gels using MOPS running buffer (ThermoFisher) and were transferred onto a nitrocellulose membrane (GE Healthcare). .. The membrane was blocked overnight at 4°C with 1:10 Western Blocking Reagent (Roche) in Tris-buffered saline, Tween 20 (TBST) buffer (20 mM Tris pH 7.5, 137 mM NaCl and 0.1% (v/v) Tween 20).

    Article Title: Unique Functional Properties of Conserved Arginine Residues in the Lentivirus Lytic Peptide Domains of the C-terminal Tail of HIV-1 gp41 *
    Article Snippet: .. The viral pellet was then resuspended in MOPS and NuPAGE SDS-PAGE buffer, heated for 10 min at 70 °C, and loaded onto NuPAGE 4–12% bis-tris gel (Invitrogen). .. Gels were electrophoresed for 50 min at 200 volts followed by transfer on PVDF membrane using the iBlot system (Invitrogen).

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    Thermo Fisher nupage mops running buffer
    ( a ) The trace from a typical size-exclusion run of purified base is shown. The peak between the void and the assembled base contains misassembled base and on a gel looks similar to properly assembled base. ( b ) Gel samples from a typical base purification run on a 10% <t>NuPAGE</t> gel in <t>MOPS</t> buffer and stained using Coomassie blue
    Nupage Mops Running Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 367 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nupage mops running buffer/product/Thermo Fisher
    Average 99 stars, based on 367 article reviews
    Price from $9.99 to $1999.99
    nupage mops running buffer - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    ( a ) The trace from a typical size-exclusion run of purified base is shown. The peak between the void and the assembled base contains misassembled base and on a gel looks similar to properly assembled base. ( b ) Gel samples from a typical base purification run on a 10% NuPAGE gel in MOPS buffer and stained using Coomassie blue

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: Recombinant Expression, Unnatural Amino Acid Incorporation, and Site-Specific Labeling of 26S Proteasomal Subcomplexes

    doi: 10.1007/978-1-4939-8706-1_15

    Figure Lengend Snippet: ( a ) The trace from a typical size-exclusion run of purified base is shown. The peak between the void and the assembled base contains misassembled base and on a gel looks similar to properly assembled base. ( b ) Gel samples from a typical base purification run on a 10% NuPAGE gel in MOPS buffer and stained using Coomassie blue

    Article Snippet: 20 × NuPAGE MOPS running buffer (Thermo Fisher).

    Techniques: Purification, Staining

    ( a ) The trace from a typical size-exclusion run of purified lid is shown. The peak after the assembled lid contains mostly cleaved MBP. ( b ) Gel samples from a typical lid purification run on a 10% NuPAGE gel in MOPS buffer and stained using Coomassie blue

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: Recombinant Expression, Unnatural Amino Acid Incorporation, and Site-Specific Labeling of 26S Proteasomal Subcomplexes

    doi: 10.1007/978-1-4939-8706-1_15

    Figure Lengend Snippet: ( a ) The trace from a typical size-exclusion run of purified lid is shown. The peak after the assembled lid contains mostly cleaved MBP. ( b ) Gel samples from a typical lid purification run on a 10% NuPAGE gel in MOPS buffer and stained using Coomassie blue

    Article Snippet: 20 × NuPAGE MOPS running buffer (Thermo Fisher).

    Techniques: Purification, Staining

    Analysis of A244_N332-rgp120 secreted from stable MGAT1 - CHO cell lines. Six stable MGAT1 - CHO cell lines identified with the ClonePix2 were selected as potential substrates for HIV vaccine production. (A) Immunoblot of affinity-purified rgp120 (50 ng per lane) produced by each of six A244_N332-rgp120 cell lines: 3E, 5C, 5D, 3F, 6B, and 5F. Purified A244_N332-rgp120 produced in normal CHO DG44 cells (692) was shown for purpose of comparison. (B) Comparison of A244_N332-rgp120 protein yields as determined by ELISA from the six MGAT1 - CHO cell lines. ( C) SDS PAGE of rgp120 produced by the 5F MGAT1 - CHO cell line. Supernatant samples (10 μ l per lane) collected over the time course of the culture were electrophoresed on a 4–12% NuPage PAGE SDS gel in MOPS buffer (Thermo Scientific, Waltham, MA). The gel was stained with Simply Blue (Thermo Scientific, Waltham, MA) and visualized using an Innotech FluoChem2 system (Genetic Technologies, Grover, MO).

    Journal: PLoS ONE

    Article Title: Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine production

    doi: 10.1371/journal.pone.0197656

    Figure Lengend Snippet: Analysis of A244_N332-rgp120 secreted from stable MGAT1 - CHO cell lines. Six stable MGAT1 - CHO cell lines identified with the ClonePix2 were selected as potential substrates for HIV vaccine production. (A) Immunoblot of affinity-purified rgp120 (50 ng per lane) produced by each of six A244_N332-rgp120 cell lines: 3E, 5C, 5D, 3F, 6B, and 5F. Purified A244_N332-rgp120 produced in normal CHO DG44 cells (692) was shown for purpose of comparison. (B) Comparison of A244_N332-rgp120 protein yields as determined by ELISA from the six MGAT1 - CHO cell lines. ( C) SDS PAGE of rgp120 produced by the 5F MGAT1 - CHO cell line. Supernatant samples (10 μ l per lane) collected over the time course of the culture were electrophoresed on a 4–12% NuPage PAGE SDS gel in MOPS buffer (Thermo Scientific, Waltham, MA). The gel was stained with Simply Blue (Thermo Scientific, Waltham, MA) and visualized using an Innotech FluoChem2 system (Genetic Technologies, Grover, MO).

    Article Snippet: For molecular mass analysis post digestion, 2 μg of each protein was electrophoresed on a 4–12% Bis-Tris PAGE SDS gel in MOPS running buffer, and stained with Coomassie Simply Blue (Thermo Scientific, Waltham, MA).

    Techniques: Affinity Purification, Produced, Purification, Enzyme-linked Immunosorbent Assay, SDS Page, Polyacrylamide Gel Electrophoresis, SDS-Gel, Staining

    Construction and imaging of GFP-Mos1 fusion transposase. ( A ) Mos1 and GFP-Mos1 transposase fusion protein constructs in pET30a expression vector. ( B ) Coomassie stained 12% SDS-PAGE in Tris-Glycine-SDS buffer of purified Mos1 and GFP-Mos1 transposases. ( C ) Live cell imaging with 60× magnification after transfection of HeLa cells with purified GFP-Mos1 transposase in complex with donor DNA. Scale bar is 10 μm. Images are representatives of 10 image points on a dish. ( D ) Quantification of the GFP signal in the nucleus and cytoplasm over time after transfection; 10 cells were analyzed. Shadowed error bars represent the standard deviation. ( E ) Western blotting analysis of HeLa cells lysates after transfection with GFP-Mos1 and donor plasmid. Proteins were separated on 4–12% NuPAGE SDS-PAGE in MOPS buffer (buffer composition, acrylamide percentage and protein concentration affect protein migration). Lane 1–1 ng of purified GFP, lane 2–10 ng of purified GFP-Mos1, lane 3-mock, no transposase or DNA was added to the cells. In lanes 3–7, 250 μg of total HeLa cell extracts were loaded. Lanes 4–7 contain full-length GFP-Mos1 transposase with no evidence of protein degradation 20 h post transfection. The antibody against GFP recognizes an unspecific protein, which is present in all time points and the mock control. Lane 7 shows lower signal for GFP-Mos1 protein since the cells have undergone division, which dilutes GFP-Mos1 concentration versus total protein concentration.

    Journal: Nucleic Acids Research

    Article Title: Use of mariner transposases for one-step delivery and integration of DNA in prokaryotes and eukaryotes by transfection

    doi: 10.1093/nar/gkx113

    Figure Lengend Snippet: Construction and imaging of GFP-Mos1 fusion transposase. ( A ) Mos1 and GFP-Mos1 transposase fusion protein constructs in pET30a expression vector. ( B ) Coomassie stained 12% SDS-PAGE in Tris-Glycine-SDS buffer of purified Mos1 and GFP-Mos1 transposases. ( C ) Live cell imaging with 60× magnification after transfection of HeLa cells with purified GFP-Mos1 transposase in complex with donor DNA. Scale bar is 10 μm. Images are representatives of 10 image points on a dish. ( D ) Quantification of the GFP signal in the nucleus and cytoplasm over time after transfection; 10 cells were analyzed. Shadowed error bars represent the standard deviation. ( E ) Western blotting analysis of HeLa cells lysates after transfection with GFP-Mos1 and donor plasmid. Proteins were separated on 4–12% NuPAGE SDS-PAGE in MOPS buffer (buffer composition, acrylamide percentage and protein concentration affect protein migration). Lane 1–1 ng of purified GFP, lane 2–10 ng of purified GFP-Mos1, lane 3-mock, no transposase or DNA was added to the cells. In lanes 3–7, 250 μg of total HeLa cell extracts were loaded. Lanes 4–7 contain full-length GFP-Mos1 transposase with no evidence of protein degradation 20 h post transfection. The antibody against GFP recognizes an unspecific protein, which is present in all time points and the mock control. Lane 7 shows lower signal for GFP-Mos1 protein since the cells have undergone division, which dilutes GFP-Mos1 concentration versus total protein concentration.

    Article Snippet: Subsequently, 250 μg of total protein extracts or recombinant purified proteins (GFP: 1 ng per lane, GFP-Mos1: 10 ng per lane) were separated on 4–12% NuPAGE SDS-PAGE gels using MOPS running buffer (ThermoFisher) and were transferred onto a nitrocellulose membrane (GE Healthcare).

    Techniques: Imaging, Construct, Expressing, Plasmid Preparation, Staining, SDS Page, Purification, Live Cell Imaging, Transfection, Standard Deviation, Western Blot, Protein Concentration, Migration, Concentration Assay