nunc microwell  (Thermo Fisher)


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    Name:
    Nunc MicroWell Plates
    Description:
    Improve efficiency in mixing washing and recovery of samples with Thermo Scientific Nunc 96 Well Polystyrene Round Bottom Microwell Plates The clear and U shaped wells are ideal for solution based assays e g agglutination assays and common serial dilutions allowing clean and easy pipetting Available in a variety of options for surfaces sterility closures and packaging sizes
    Catalog Number:
    143761
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    Applications:
    Cell Culture|Cell Culture Plastics
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    Lab Supplies Plastics Glassware
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    Structured Review

    Thermo Fisher nunc microwell
    Improve efficiency in mixing washing and recovery of samples with Thermo Scientific Nunc 96 Well Polystyrene Round Bottom Microwell Plates The clear and U shaped wells are ideal for solution based assays e g agglutination assays and common serial dilutions allowing clean and easy pipetting Available in a variety of options for surfaces sterility closures and packaging sizes
    https://www.bioz.com/result/nunc microwell/product/Thermo Fisher
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nunc microwell - by Bioz Stars, 2020-07
    96/100 stars

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    Related Articles

    MTT Assay:

    Article Title: Co-culture with lung cancer A549 cells promotes the proliferation and migration of mesenchymal stem cells derived from bone marrow
    Article Snippet: .. Cells (~1×104 cells/well) were incubated in 96-microwell plates (Nalge Nunc International, Penfield, NY, USA) at 37°C for 24 h. Following incubation, 20 µl MTT solution was added to each well and incubated for 4 h in a 5% CO2 incubator at 37°C. .. Subsequently, the solution was removed and the construct was blotted with filter paper.

    Transfection:

    Article Title: Drug discovery with an RBM20 dependent titin splice reporter identifies cardenolides as lead structures to improve cardiac filling
    Article Snippet: .. For transfection 25000 cells/well were seeded on 96-well Nunc F96 MicroWell™ plates (Life Technologies GmbH, Darmstadt, Germany) and transfected with a total of 200 ng of plasmid DNA of which 1 ng was splice reporter plus a corresponding amount of RBM20, RBFOX1 or control plasmid (pcDNA3.1) in a 20x molar excess. .. To deliver plasmid DNA we used the 40 kDa linear polyethylenimine (Polysciences Europe GmbH, Hirschberg, Germany) at a 1:3 ratio (DNA:PEI40).

    Fluorescence:

    Article Title: Fluorescence Quenching-Based Mechanism for Determination of Hypochlorite by Coumarin-Derived Sensors
    Article Snippet: .. Steady-state fluorescence measurements were performed in 96-well black plates (Nunc™ F96 MicroWell™ Black Polystyrene Plate, Thermo Scientific™, Roskilde, Denmark) on a Tecan Infinite 200 microplate reader (Tecan Austria GmbH, Grödig/Salzburg, Austria). .. HPLC-ESI-MS analyses were performed on an LCMS-8030 mass spectrometer (Shimadzu, Kyoto, Japan).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Development of a Whole-Virus ELISA for Serological Evaluation of Domestic Livestock as Possible Hosts of Human Coronavirus NL63
    Article Snippet: .. Final ELISA Testing Procedure In brief, viral protein was coated into 96-well Nunc MicroWellTM plates (Thermo Fisher, US) by diluting the stock to 0.75 µg/mL in NaCO3 buffer (0.1 M, PH 9.6) and coating with 50 µL per well with overnight incubation at 4 °C. .. The plates were washed 5 times with 0.1% PBS-T then blocked with 5% milk powder (Carl Roth, Germany) in PBS-T for one hour at room temperature and the wash repeated.

    Incubation:

    Article Title: Induction and maintenance of anti‐influenza antigen‐specific nasal secretory IgA levels and serum IgG levels after influenza infection in adults
    Article Snippet: .. For quantification of anti‐IAV‐specific s‐IgA in nasal washes, 96‐MicroWell Plates (Nalge Nunc International, Tokyo, Japan) were coated with the IAV vaccine antigens (0·1 μg/well) described previously, incubated overnight in phosphate‐buffered saline (PBS) at 4°C, and then blocked with 1% BSA in 50 m m Tris–HCl (pH 8·0) containing 0·14 m NaCl and 0·05% Tween‐20 (TTBS) for 1 h at room temperature. .. The nasal wash diluted with TTBS was added to each well and incubated for 2 h at room temperature.

    Article Title: Co-culture with lung cancer A549 cells promotes the proliferation and migration of mesenchymal stem cells derived from bone marrow
    Article Snippet: .. Cells (~1×104 cells/well) were incubated in 96-microwell plates (Nalge Nunc International, Penfield, NY, USA) at 37°C for 24 h. Following incubation, 20 µl MTT solution was added to each well and incubated for 4 h in a 5% CO2 incubator at 37°C. .. Subsequently, the solution was removed and the construct was blotted with filter paper.

    Article Title: Development of a Whole-Virus ELISA for Serological Evaluation of Domestic Livestock as Possible Hosts of Human Coronavirus NL63
    Article Snippet: .. Final ELISA Testing Procedure In brief, viral protein was coated into 96-well Nunc MicroWellTM plates (Thermo Fisher, US) by diluting the stock to 0.75 µg/mL in NaCO3 buffer (0.1 M, PH 9.6) and coating with 50 µL per well with overnight incubation at 4 °C. .. The plates were washed 5 times with 0.1% PBS-T then blocked with 5% milk powder (Carl Roth, Germany) in PBS-T for one hour at room temperature and the wash repeated.

    Plasmid Preparation:

    Article Title: Drug discovery with an RBM20 dependent titin splice reporter identifies cardenolides as lead structures to improve cardiac filling
    Article Snippet: .. For transfection 25000 cells/well were seeded on 96-well Nunc F96 MicroWell™ plates (Life Technologies GmbH, Darmstadt, Germany) and transfected with a total of 200 ng of plasmid DNA of which 1 ng was splice reporter plus a corresponding amount of RBM20, RBFOX1 or control plasmid (pcDNA3.1) in a 20x molar excess. .. To deliver plasmid DNA we used the 40 kDa linear polyethylenimine (Polysciences Europe GmbH, Hirschberg, Germany) at a 1:3 ratio (DNA:PEI40).

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  • 98
    Thermo Fisher 96 well optical bottom plates
    High-Throughput Fungal-Bacterial Interaction Screen and Biocontrol Activities of Bacterial Root Commensals, Related to   Figure 5 (A) Schematic overview of the experimental protocol. Fungal spores were equally distributed to the wells of a transparent-bottom 96 well plate and incubated in the presence or absence of different root-derived bacteria (stationary phase) in liquid medium (screening plate). Bacteria were also grown in the absence of fungal spores as a control (background plate). After 48 hours of interaction, three washing steps were used to eliminate bacterial cells in suspension. Note that the fungal mycelium sticks to the transparent optical bottom of the plate. After overnight incubation in Wheat Germ Agglutinin and two additional washes, the fluorescence intensity (reflecting fungal growth) was measured using a plate reader. The relative growth index was calculated as illustrated (see   STAR Methods ). (B) Alteration of the growth of  Plectosphaerella cucumerina isolate 10  upon competition with phylogenetically diverse members of the bacterial root microbiota in minimum medium (M9) and a carbon-rich medium (20% TSB). The phylogenetic tree was constructed based on the full bacterial 16S rRNA gene sequences and bootstrap values are depicted with black circles. The heatmap depicts the log2 fungal relative growth index (presence versus absence of bacterial competitors) measured by fluorescence (see above). Note the similar overall inhibitory activities in minimum and complex media. (C) Validation experiment, in which the bacterial strains were re-screened against ten randomly-selected fungi. Fluorescence intensities (log2) were compared with those obtained from the first biological replicate. (D) Comparison of network-derived correlations and experimentally tested interactions of bacterial families with fungal species. For each bacterial family shared between antagonistic screening and root-associated OTU network analysis, the average antagonistic activity against fungal isolates and the cumulative correlation to fungal OTUs in the network are represented. Bacterial families with more than two members were considered and values for both measurements were normalized to be in the same range. (E) Direct comparison of bacterial OTUs from the root network with bacterial isolates used in the antagonistic screening. Each data point corresponds to a bacterial OTU-isolate pair ( >  97% sequence similarity; only best matching hits are shown). For each pair, the network-derived correlation with fungal OTUs (from the bacterial OTU) is plotted against the result from the antagonistic screening (from the bacterial isolates). (F) Same as E) but data are presented in a correlation plot. (G) Validation of withdrawal of a subset of bacterial strains in the FlowPot microbiota reconstitution system. Relative abundances of isolates of the Pseudomonadaceae (top) and the Comamonadaceae (bottom) families in output matrix and root samples four weeks after inoculation (direct mapping at 100% sequence similarity). RA: Relative abundance. -C: withdrawal of ten Comamonadaceae strains, -P: withdrawal of eight Pseudomonadaceae strains. -C-P removal of 18 Comamonadaceae plus Pseudomonadaceae strains. Control samples were inoculated with the full 148-member bacterial community. Note that one Acidovorax strain (Acidovorax 275) was unintentionally not removed from the -C depleted samples. This Acidovorax strain is unable to rescue plant growth in the presence of the fungal community (see   Figure 5 C). (H and I) Relative abundance of (H) fungal isolates and (I) bacterial isolates (direct mapping at 100% sequence similarity) is presented for all conditions. B: full 148-member bacterial community. F: 34-member fungal community. -C: depletion of Comamonadaceae isolates. -P: depletion of Pseudomonadaceae isolates. -C-P: depletion of Comamonadaceae and Pseudomonadaceae isolates.
    96 Well Optical Bottom Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/96 well optical bottom plates/product/Thermo Fisher
    Average 98 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    96 well optical bottom plates - by Bioz Stars, 2020-07
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    93
    Thermo Fisher luminometer compatible nunc microwell 96 well plates
    High-Throughput Fungal-Bacterial Interaction Screen and Biocontrol Activities of Bacterial Root Commensals, Related to   Figure 5 (A) Schematic overview of the experimental protocol. Fungal spores were equally distributed to the wells of a transparent-bottom 96 well plate and incubated in the presence or absence of different root-derived bacteria (stationary phase) in liquid medium (screening plate). Bacteria were also grown in the absence of fungal spores as a control (background plate). After 48 hours of interaction, three washing steps were used to eliminate bacterial cells in suspension. Note that the fungal mycelium sticks to the transparent optical bottom of the plate. After overnight incubation in Wheat Germ Agglutinin and two additional washes, the fluorescence intensity (reflecting fungal growth) was measured using a plate reader. The relative growth index was calculated as illustrated (see   STAR Methods ). (B) Alteration of the growth of  Plectosphaerella cucumerina isolate 10  upon competition with phylogenetically diverse members of the bacterial root microbiota in minimum medium (M9) and a carbon-rich medium (20% TSB). The phylogenetic tree was constructed based on the full bacterial 16S rRNA gene sequences and bootstrap values are depicted with black circles. The heatmap depicts the log2 fungal relative growth index (presence versus absence of bacterial competitors) measured by fluorescence (see above). Note the similar overall inhibitory activities in minimum and complex media. (C) Validation experiment, in which the bacterial strains were re-screened against ten randomly-selected fungi. Fluorescence intensities (log2) were compared with those obtained from the first biological replicate. (D) Comparison of network-derived correlations and experimentally tested interactions of bacterial families with fungal species. For each bacterial family shared between antagonistic screening and root-associated OTU network analysis, the average antagonistic activity against fungal isolates and the cumulative correlation to fungal OTUs in the network are represented. Bacterial families with more than two members were considered and values for both measurements were normalized to be in the same range. (E) Direct comparison of bacterial OTUs from the root network with bacterial isolates used in the antagonistic screening. Each data point corresponds to a bacterial OTU-isolate pair ( >  97% sequence similarity; only best matching hits are shown). For each pair, the network-derived correlation with fungal OTUs (from the bacterial OTU) is plotted against the result from the antagonistic screening (from the bacterial isolates). (F) Same as E) but data are presented in a correlation plot. (G) Validation of withdrawal of a subset of bacterial strains in the FlowPot microbiota reconstitution system. Relative abundances of isolates of the Pseudomonadaceae (top) and the Comamonadaceae (bottom) families in output matrix and root samples four weeks after inoculation (direct mapping at 100% sequence similarity). RA: Relative abundance. -C: withdrawal of ten Comamonadaceae strains, -P: withdrawal of eight Pseudomonadaceae strains. -C-P removal of 18 Comamonadaceae plus Pseudomonadaceae strains. Control samples were inoculated with the full 148-member bacterial community. Note that one Acidovorax strain (Acidovorax 275) was unintentionally not removed from the -C depleted samples. This Acidovorax strain is unable to rescue plant growth in the presence of the fungal community (see   Figure 5 C). (H and I) Relative abundance of (H) fungal isolates and (I) bacterial isolates (direct mapping at 100% sequence similarity) is presented for all conditions. B: full 148-member bacterial community. F: 34-member fungal community. -C: depletion of Comamonadaceae isolates. -P: depletion of Pseudomonadaceae isolates. -C-P: depletion of Comamonadaceae and Pseudomonadaceae isolates.
    Luminometer Compatible Nunc Microwell 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luminometer compatible nunc microwell 96 well plates/product/Thermo Fisher
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    luminometer compatible nunc microwell 96 well plates - by Bioz Stars, 2020-07
    93/100 stars
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    96
    Thermo Fisher thermo scientific nunc microwell
    High-Throughput Fungal-Bacterial Interaction Screen and Biocontrol Activities of Bacterial Root Commensals, Related to   Figure 5 (A) Schematic overview of the experimental protocol. Fungal spores were equally distributed to the wells of a transparent-bottom 96 well plate and incubated in the presence or absence of different root-derived bacteria (stationary phase) in liquid medium (screening plate). Bacteria were also grown in the absence of fungal spores as a control (background plate). After 48 hours of interaction, three washing steps were used to eliminate bacterial cells in suspension. Note that the fungal mycelium sticks to the transparent optical bottom of the plate. After overnight incubation in Wheat Germ Agglutinin and two additional washes, the fluorescence intensity (reflecting fungal growth) was measured using a plate reader. The relative growth index was calculated as illustrated (see   STAR Methods ). (B) Alteration of the growth of  Plectosphaerella cucumerina isolate 10  upon competition with phylogenetically diverse members of the bacterial root microbiota in minimum medium (M9) and a carbon-rich medium (20% TSB). The phylogenetic tree was constructed based on the full bacterial 16S rRNA gene sequences and bootstrap values are depicted with black circles. The heatmap depicts the log2 fungal relative growth index (presence versus absence of bacterial competitors) measured by fluorescence (see above). Note the similar overall inhibitory activities in minimum and complex media. (C) Validation experiment, in which the bacterial strains were re-screened against ten randomly-selected fungi. Fluorescence intensities (log2) were compared with those obtained from the first biological replicate. (D) Comparison of network-derived correlations and experimentally tested interactions of bacterial families with fungal species. For each bacterial family shared between antagonistic screening and root-associated OTU network analysis, the average antagonistic activity against fungal isolates and the cumulative correlation to fungal OTUs in the network are represented. Bacterial families with more than two members were considered and values for both measurements were normalized to be in the same range. (E) Direct comparison of bacterial OTUs from the root network with bacterial isolates used in the antagonistic screening. Each data point corresponds to a bacterial OTU-isolate pair ( >  97% sequence similarity; only best matching hits are shown). For each pair, the network-derived correlation with fungal OTUs (from the bacterial OTU) is plotted against the result from the antagonistic screening (from the bacterial isolates). (F) Same as E) but data are presented in a correlation plot. (G) Validation of withdrawal of a subset of bacterial strains in the FlowPot microbiota reconstitution system. Relative abundances of isolates of the Pseudomonadaceae (top) and the Comamonadaceae (bottom) families in output matrix and root samples four weeks after inoculation (direct mapping at 100% sequence similarity). RA: Relative abundance. -C: withdrawal of ten Comamonadaceae strains, -P: withdrawal of eight Pseudomonadaceae strains. -C-P removal of 18 Comamonadaceae plus Pseudomonadaceae strains. Control samples were inoculated with the full 148-member bacterial community. Note that one Acidovorax strain (Acidovorax 275) was unintentionally not removed from the -C depleted samples. This Acidovorax strain is unable to rescue plant growth in the presence of the fungal community (see   Figure 5 C). (H and I) Relative abundance of (H) fungal isolates and (I) bacterial isolates (direct mapping at 100% sequence similarity) is presented for all conditions. B: full 148-member bacterial community. F: 34-member fungal community. -C: depletion of Comamonadaceae isolates. -P: depletion of Pseudomonadaceae isolates. -C-P: depletion of Comamonadaceae and Pseudomonadaceae isolates.
    Thermo Scientific Nunc Microwell, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermo scientific nunc microwell/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thermo scientific nunc microwell - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

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    High-Throughput Fungal-Bacterial Interaction Screen and Biocontrol Activities of Bacterial Root Commensals, Related to   Figure 5 (A) Schematic overview of the experimental protocol. Fungal spores were equally distributed to the wells of a transparent-bottom 96 well plate and incubated in the presence or absence of different root-derived bacteria (stationary phase) in liquid medium (screening plate). Bacteria were also grown in the absence of fungal spores as a control (background plate). After 48 hours of interaction, three washing steps were used to eliminate bacterial cells in suspension. Note that the fungal mycelium sticks to the transparent optical bottom of the plate. After overnight incubation in Wheat Germ Agglutinin and two additional washes, the fluorescence intensity (reflecting fungal growth) was measured using a plate reader. The relative growth index was calculated as illustrated (see   STAR Methods ). (B) Alteration of the growth of  Plectosphaerella cucumerina isolate 10  upon competition with phylogenetically diverse members of the bacterial root microbiota in minimum medium (M9) and a carbon-rich medium (20% TSB). The phylogenetic tree was constructed based on the full bacterial 16S rRNA gene sequences and bootstrap values are depicted with black circles. The heatmap depicts the log2 fungal relative growth index (presence versus absence of bacterial competitors) measured by fluorescence (see above). Note the similar overall inhibitory activities in minimum and complex media. (C) Validation experiment, in which the bacterial strains were re-screened against ten randomly-selected fungi. Fluorescence intensities (log2) were compared with those obtained from the first biological replicate. (D) Comparison of network-derived correlations and experimentally tested interactions of bacterial families with fungal species. For each bacterial family shared between antagonistic screening and root-associated OTU network analysis, the average antagonistic activity against fungal isolates and the cumulative correlation to fungal OTUs in the network are represented. Bacterial families with more than two members were considered and values for both measurements were normalized to be in the same range. (E) Direct comparison of bacterial OTUs from the root network with bacterial isolates used in the antagonistic screening. Each data point corresponds to a bacterial OTU-isolate pair ( >  97% sequence similarity; only best matching hits are shown). For each pair, the network-derived correlation with fungal OTUs (from the bacterial OTU) is plotted against the result from the antagonistic screening (from the bacterial isolates). (F) Same as E) but data are presented in a correlation plot. (G) Validation of withdrawal of a subset of bacterial strains in the FlowPot microbiota reconstitution system. Relative abundances of isolates of the Pseudomonadaceae (top) and the Comamonadaceae (bottom) families in output matrix and root samples four weeks after inoculation (direct mapping at 100% sequence similarity). RA: Relative abundance. -C: withdrawal of ten Comamonadaceae strains, -P: withdrawal of eight Pseudomonadaceae strains. -C-P removal of 18 Comamonadaceae plus Pseudomonadaceae strains. Control samples were inoculated with the full 148-member bacterial community. Note that one Acidovorax strain (Acidovorax 275) was unintentionally not removed from the -C depleted samples. This Acidovorax strain is unable to rescue plant growth in the presence of the fungal community (see   Figure 5 C). (H and I) Relative abundance of (H) fungal isolates and (I) bacterial isolates (direct mapping at 100% sequence similarity) is presented for all conditions. B: full 148-member bacterial community. F: 34-member fungal community. -C: depletion of Comamonadaceae isolates. -P: depletion of Pseudomonadaceae isolates. -C-P: depletion of Comamonadaceae and Pseudomonadaceae isolates.

    Journal: Cell

    Article Title: Microbial Interkingdom Interactions in Roots Promote Arabidopsis Survival

    doi: 10.1016/j.cell.2018.10.020

    Figure Lengend Snippet: High-Throughput Fungal-Bacterial Interaction Screen and Biocontrol Activities of Bacterial Root Commensals, Related to Figure 5 (A) Schematic overview of the experimental protocol. Fungal spores were equally distributed to the wells of a transparent-bottom 96 well plate and incubated in the presence or absence of different root-derived bacteria (stationary phase) in liquid medium (screening plate). Bacteria were also grown in the absence of fungal spores as a control (background plate). After 48 hours of interaction, three washing steps were used to eliminate bacterial cells in suspension. Note that the fungal mycelium sticks to the transparent optical bottom of the plate. After overnight incubation in Wheat Germ Agglutinin and two additional washes, the fluorescence intensity (reflecting fungal growth) was measured using a plate reader. The relative growth index was calculated as illustrated (see STAR Methods ). (B) Alteration of the growth of Plectosphaerella cucumerina isolate 10 upon competition with phylogenetically diverse members of the bacterial root microbiota in minimum medium (M9) and a carbon-rich medium (20% TSB). The phylogenetic tree was constructed based on the full bacterial 16S rRNA gene sequences and bootstrap values are depicted with black circles. The heatmap depicts the log2 fungal relative growth index (presence versus absence of bacterial competitors) measured by fluorescence (see above). Note the similar overall inhibitory activities in minimum and complex media. (C) Validation experiment, in which the bacterial strains were re-screened against ten randomly-selected fungi. Fluorescence intensities (log2) were compared with those obtained from the first biological replicate. (D) Comparison of network-derived correlations and experimentally tested interactions of bacterial families with fungal species. For each bacterial family shared between antagonistic screening and root-associated OTU network analysis, the average antagonistic activity against fungal isolates and the cumulative correlation to fungal OTUs in the network are represented. Bacterial families with more than two members were considered and values for both measurements were normalized to be in the same range. (E) Direct comparison of bacterial OTUs from the root network with bacterial isolates used in the antagonistic screening. Each data point corresponds to a bacterial OTU-isolate pair ( > 97% sequence similarity; only best matching hits are shown). For each pair, the network-derived correlation with fungal OTUs (from the bacterial OTU) is plotted against the result from the antagonistic screening (from the bacterial isolates). (F) Same as E) but data are presented in a correlation plot. (G) Validation of withdrawal of a subset of bacterial strains in the FlowPot microbiota reconstitution system. Relative abundances of isolates of the Pseudomonadaceae (top) and the Comamonadaceae (bottom) families in output matrix and root samples four weeks after inoculation (direct mapping at 100% sequence similarity). RA: Relative abundance. -C: withdrawal of ten Comamonadaceae strains, -P: withdrawal of eight Pseudomonadaceae strains. -C-P removal of 18 Comamonadaceae plus Pseudomonadaceae strains. Control samples were inoculated with the full 148-member bacterial community. Note that one Acidovorax strain (Acidovorax 275) was unintentionally not removed from the -C depleted samples. This Acidovorax strain is unable to rescue plant growth in the presence of the fungal community (see Figure 5 C). (H and I) Relative abundance of (H) fungal isolates and (I) bacterial isolates (direct mapping at 100% sequence similarity) is presented for all conditions. B: full 148-member bacterial community. F: 34-member fungal community. -C: depletion of Comamonadaceae isolates. -P: depletion of Pseudomonadaceae isolates. -C-P: depletion of Comamonadaceae and Pseudomonadaceae isolates.

    Article Snippet: Two 96-well optical bottom plates (ThermoFisher Scientific/Nunc, Rochester, USA) were used for the screening.

    Techniques: High Throughput Screening Assay, Incubation, Derivative Assay, Fluorescence, Construct, Activity Assay, Sequencing