nunc cell culture treated flasks  (Thermo Fisher)


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    Name:
    Nunc Cell Culture Treated Flasks
    Description:
    Achieve maximum adhesion for a broad range of cell types with Thermo Scientific Nunc Cell Culture Treated Flasks with Filter Caps These flasks receive the proprietary Nunclon Delta surface treatment to ensure consistent cell growth The filter caps allow constant airflow and minimize chances of contamination
    Catalog Number:
    136196
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    Applications:
    Cell Culture|Cell Culture Plastics|Laboratory Plastics and Supplies|Microplates, Dishes and Flasks
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    Structured Review

    Thermo Fisher nunc cell culture treated flasks
    Achieve maximum adhesion for a broad range of cell types with Thermo Scientific Nunc Cell Culture Treated Flasks with Filter Caps These flasks receive the proprietary Nunclon Delta surface treatment to ensure consistent cell growth The filter caps allow constant airflow and minimize chances of contamination
    https://www.bioz.com/result/nunc cell culture treated flasks/product/Thermo Fisher
    Average 90 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    nunc cell culture treated flasks - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Amphibian chytridiomycosis outbreak dynamics are linked with host skin bacterial community structure
    Article Snippet: Isolates were grown in 50 ml Nalgene Nunc™ tissue culture-treated flasks (Thermo Fisher Scientific, Massachusetts, USA) for 10–14 days at 18–20 °C. .. DNA extraction was performed using the MasterPure™ Yeast DNA Purification Kit (Epicentre, Wisconsin, USA) or Qiagen Genomic Tips 20/G and DNeasy Blood and Tissue Kits (Qiagen, Venlo, Netherlands).

    Transduction:

    Article Title: Tumor Infiltrating Lymphocytes Genetically Engineered with an Inducible Gene Encoding Interleukin-12 for the Immunotherapy of Metastatic Melanoma
    Article Snippet: .. After transduction, cells were transferred from the 6 well plates into 175cm2 Nunc Cell Culture Treated Flasks with Filter Caps (Thermo Scientific) at a concentration of 0.5×106 cells/ml. .. Cells were cultured in AIMV media (Life technologies) supplemented with 3000 IU/ml Aldesleukin (IL-2; Novartis), 2mM Glutamax (Life technologies), 5% human serum (Gemini BioProducts) 100U/ml penicillin and 100μg/ml streptomycin (Lonza).

    Transfection:

    Article Title: Optimizing assembly and production of native bispecific antibodies by codon de-optimization
    Article Snippet: .. One day before transfection, confluent cells were split 1:4 in T175 flasks (Nunc™ Cell Culture Treated Flasks with Filter Caps, Thermo Fisher Scientific) to have cells in the exponential growth phase. .. Transient transfections were performed using a mix containing 30 μg of DNA and 42 μL of Lipofectamine® 2000 transfection reagent (Thermo Fisher Scientific) in 1.4 mL of DMEM for 107 cells per T175 flask in 50 mL of complete DMEM.

    Article Title: Tuning Relative Polypeptide Expression to Optimize Assembly, Yield and Downstream Processing of Bispecific Antibodies
    Article Snippet: .. One day before transfection, confluent cells were splited 1:4 in T175 flasks (Nunc™ Cell Culture Treated Flasks with Filter Caps, Thermo Scientific, Waltham, MA, USA) in order to have cells in the exponential growth phase. .. Transient transfections were performed using a mix containing 30 µg of DNA and 42 µL of Lipofectamine 2000 transfection reagent (Invitrogen, Carlsbad, CA, USA) in 1.4 mL of DMEM for 107 cells per T175 flask in 50 mL of complete DMEM.

    Sequencing:

    Article Title: Amphibian chytridiomycosis outbreak dynamics are linked with host skin bacterial community structure
    Article Snippet: Paragraph title: Bd isolation and genome sequencing ... Isolates were grown in 50 ml Nalgene Nunc™ tissue culture-treated flasks (Thermo Fisher Scientific, Massachusetts, USA) for 10–14 days at 18–20 °C.

    DNA Purification:

    Article Title: Amphibian chytridiomycosis outbreak dynamics are linked with host skin bacterial community structure
    Article Snippet: Isolates were grown in 50 ml Nalgene Nunc™ tissue culture-treated flasks (Thermo Fisher Scientific, Massachusetts, USA) for 10–14 days at 18–20 °C. .. DNA extraction was performed using the MasterPure™ Yeast DNA Purification Kit (Epicentre, Wisconsin, USA) or Qiagen Genomic Tips 20/G and DNeasy Blood and Tissue Kits (Qiagen, Venlo, Netherlands).

    Concentration Assay:

    Article Title: Reparative macrophage transplantation for myocardial repair: a refinement of bone marrow mononuclear cell-based therapy
    Article Snippet: .. M2-like macrophage generation protocol Freshly isolated BM-MNCs were seeded onto Nunc© Cell Culture Treated Flasks with Filter Caps (ThermoFisher) at a concentration of 3.6 × 104 cells/cm2 . .. The cells were treated by supplementing complete DMEM with M-CSF (20 ng/ml; Peprotech) and either IL-4, IL-10, TGF-β1 (20 ng/ml; Peprotech) or specific combinations of them.

    Article Title: Tumor Infiltrating Lymphocytes Genetically Engineered with an Inducible Gene Encoding Interleukin-12 for the Immunotherapy of Metastatic Melanoma
    Article Snippet: .. After transduction, cells were transferred from the 6 well plates into 175cm2 Nunc Cell Culture Treated Flasks with Filter Caps (Thermo Scientific) at a concentration of 0.5×106 cells/ml. .. Cells were cultured in AIMV media (Life technologies) supplemented with 3000 IU/ml Aldesleukin (IL-2; Novartis), 2mM Glutamax (Life technologies), 5% human serum (Gemini BioProducts) 100U/ml penicillin and 100μg/ml streptomycin (Lonza).

    Isolation:

    Article Title: Reparative macrophage transplantation for myocardial repair: a refinement of bone marrow mononuclear cell-based therapy
    Article Snippet: .. M2-like macrophage generation protocol Freshly isolated BM-MNCs were seeded onto Nunc© Cell Culture Treated Flasks with Filter Caps (ThermoFisher) at a concentration of 3.6 × 104 cells/cm2 . .. The cells were treated by supplementing complete DMEM with M-CSF (20 ng/ml; Peprotech) and either IL-4, IL-10, TGF-β1 (20 ng/ml; Peprotech) or specific combinations of them.

    Article Title: Amphibian chytridiomycosis outbreak dynamics are linked with host skin bacterial community structure
    Article Snippet: Paragraph title: Bd isolation and genome sequencing ... Isolates were grown in 50 ml Nalgene Nunc™ tissue culture-treated flasks (Thermo Fisher Scientific, Massachusetts, USA) for 10–14 days at 18–20 °C.

    Article Title: Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica
    Article Snippet: Culture procedures For viral isolation, we used Acanthamoeba polyphaga (ATCC 30461), Acanthamoeba castellanii (ATCC 30234) kindly provided by the Laboratório de Amebíases (Departamento de Parasitologia, ICB/UFMG) and Vermamoeba vermiformis (ATCC CDC19), kindly provided by Professor Bernard La Scola from Aix Marseille University. .. Amoeba were grown in 75 cm2 Nunc™ Cell Culture Treated Flasks with Filter Caps (Thermo Fisher Scientific, USA) with 30 mL of peptone-yeast extract-glucose (PYG) medium supplemented with 0,14 mg/mL penicillin (Sigma-Aldrich, USA), 50 mg/mL gentamycin (Thermo Fisher Scientific, USA) and 2.5 mg/mL amphotericin (Bristol-Myers- Squibb, New York, USA) at 32 °C.

    Article Title: Therapeutic Benefits of Young, But Not Old, Adipose-Derived Mesenchymal Stem Cells in a Chronic Mouse Model of Bleomycin-Induced Pulmonary Fibrosis
    Article Snippet: Paragraph title: ASC Isolation ... Cells were expanded in plastic Thermo Scientific™ Nunc™ Cell Culture Treated Flasks with Filter Caps (Thermo Fisher Scientific, Inc., Waltham, MA).

    Cell Culture:

    Article Title: Reparative macrophage transplantation for myocardial repair: a refinement of bone marrow mononuclear cell-based therapy
    Article Snippet: .. M2-like macrophage generation protocol Freshly isolated BM-MNCs were seeded onto Nunc© Cell Culture Treated Flasks with Filter Caps (ThermoFisher) at a concentration of 3.6 × 104 cells/cm2 . .. The cells were treated by supplementing complete DMEM with M-CSF (20 ng/ml; Peprotech) and either IL-4, IL-10, TGF-β1 (20 ng/ml; Peprotech) or specific combinations of them.

    Article Title: Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica
    Article Snippet: .. The contents of wells with cytopathic effect were collected and inoculated in a new 25 cm2 Nunc™ Cell Culture Treated Flasks with Filter Caps (Thermo Fisher Scientific, USA) culture containing 1 million cells, the cytopathic effect was confirmed and this culture was centrifuged 10,000 rpm for 10 min (Centrifuge Sigma 1–14) for lysate clearance and were further analyzed for giant viruses. ..

    Article Title: Utilization of lactic acid in human myotubes and interplay with glucose and fatty acid metabolism
    Article Snippet: .. Nunc™ Cell Culture Treated Flasks with Filter Caps, Nunc™ 96-MicroWell™ plates, TaqMan reverse transcription kit reagents, High-Capacity cDNA Reverse Transcription Kit, primers for TaqMan PCR, MicroAmp® Optical 96-well Reaction Plate, MicroAmp® Optical Adhesive Film, and Power SYBR® Green PCR Master Mix were obtained from ThermoFisher Scientific (Roskilde, Denmark). .. Glycerol, Tris-HCl and thin layer chromatography plates were from Merck (Darmstadt, Germany).

    Article Title: Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica
    Article Snippet: .. Amoeba were grown in 75 cm2 Nunc™ Cell Culture Treated Flasks with Filter Caps (Thermo Fisher Scientific, USA) with 30 mL of peptone-yeast extract-glucose (PYG) medium supplemented with 0,14 mg/mL penicillin (Sigma-Aldrich, USA), 50 mg/mL gentamycin (Thermo Fisher Scientific, USA) and 2.5 mg/mL amphotericin (Bristol-Myers- Squibb, New York, USA) at 32 °C. .. For co-culture, amoeba were re-suspended in 10 mL of PYG supplemented with an antibiotic mix containing 0,004 mg/mL ciprofloxacin (Cellofarm, Brazil), 0,004 mg/mL vancomycin (Sigma-Aldrich, U.S.A), and 0,020 mg/mL doxycycline (Sigma-Aldrich, U.S.A).

    Article Title: Tumor Infiltrating Lymphocytes Genetically Engineered with an Inducible Gene Encoding Interleukin-12 for the Immunotherapy of Metastatic Melanoma
    Article Snippet: .. After transduction, cells were transferred from the 6 well plates into 175cm2 Nunc Cell Culture Treated Flasks with Filter Caps (Thermo Scientific) at a concentration of 0.5×106 cells/ml. .. Cells were cultured in AIMV media (Life technologies) supplemented with 3000 IU/ml Aldesleukin (IL-2; Novartis), 2mM Glutamax (Life technologies), 5% human serum (Gemini BioProducts) 100U/ml penicillin and 100μg/ml streptomycin (Lonza).

    Article Title: Trimethylated homoserine functions as the major compatible solute in the globally significant oceanic cyanobacterium Trichodesmium
    Article Snippet: .. T. erythraeum strain IMS101 was grown in 160 mL of Nunc Cell Culture Treated EasYFlasks with filter screw caps (Thermo Fisher Scientific) in artificial seawater YCBII medium ( ). .. The light regime was a 12-h-light/12-h-dark cycle with light of 22 μmol photons m−2 ⋅s−1 .

    Article Title: Optimizing assembly and production of native bispecific antibodies by codon de-optimization
    Article Snippet: .. One day before transfection, confluent cells were split 1:4 in T175 flasks (Nunc™ Cell Culture Treated Flasks with Filter Caps, Thermo Fisher Scientific) to have cells in the exponential growth phase. .. Transient transfections were performed using a mix containing 30 μg of DNA and 42 μL of Lipofectamine® 2000 transfection reagent (Thermo Fisher Scientific) in 1.4 mL of DMEM for 107 cells per T175 flask in 50 mL of complete DMEM.

    Article Title: Therapeutic Benefits of Young, But Not Old, Adipose-Derived Mesenchymal Stem Cells in a Chronic Mouse Model of Bleomycin-Induced Pulmonary Fibrosis
    Article Snippet: .. Cells were expanded in plastic Thermo Scientific™ Nunc™ Cell Culture Treated Flasks with Filter Caps (Thermo Fisher Scientific, Inc., Waltham, MA). .. After a 24-hr incubation period, non-adherent cells were removed.

    Article Title: Tracing information flow from Erk to target gene induction reveals mechanisms of dynamic and combinatorial control
    Article Snippet: .. Cells were maintained on Thermo Scientific™ Nunc™ Cell Culture Treated Flasks with Filter Caps and grown at 37°C with 5% CO2 . ..

    Article Title: Tuning Relative Polypeptide Expression to Optimize Assembly, Yield and Downstream Processing of Bispecific Antibodies
    Article Snippet: .. One day before transfection, confluent cells were splited 1:4 in T175 flasks (Nunc™ Cell Culture Treated Flasks with Filter Caps, Thermo Scientific, Waltham, MA, USA) in order to have cells in the exponential growth phase. .. Transient transfections were performed using a mix containing 30 µg of DNA and 42 µL of Lipofectamine 2000 transfection reagent (Invitrogen, Carlsbad, CA, USA) in 1.4 mL of DMEM for 107 cells per T175 flask in 50 mL of complete DMEM.

    Article Title: The Biochemistry and Immunohistochemistry of Versican
    Article Snippet: .. We have used Nunc™ Cell Culture Treated TripleFlasks (Thermo-Fisher) with good results. .. Collect conditioned cell culture medium (DMEM containing 10 % FBS) from cultures of aortic smooth muscle cells grown over several passages.

    Mouse Assay:

    Article Title: Therapeutic Benefits of Young, But Not Old, Adipose-Derived Mesenchymal Stem Cells in a Chronic Mouse Model of Bleomycin-Induced Pulmonary Fibrosis
    Article Snippet: Mice were anesthetized by a ketamine (200 mg/kg) and xylazine (10 mg/kg) injected intraperitoneally. .. Cells were expanded in plastic Thermo Scientific™ Nunc™ Cell Culture Treated Flasks with Filter Caps (Thermo Fisher Scientific, Inc., Waltham, MA).

    SYBR Green Assay:

    Article Title: Utilization of lactic acid in human myotubes and interplay with glucose and fatty acid metabolism
    Article Snippet: .. Nunc™ Cell Culture Treated Flasks with Filter Caps, Nunc™ 96-MicroWell™ plates, TaqMan reverse transcription kit reagents, High-Capacity cDNA Reverse Transcription Kit, primers for TaqMan PCR, MicroAmp® Optical 96-well Reaction Plate, MicroAmp® Optical Adhesive Film, and Power SYBR® Green PCR Master Mix were obtained from ThermoFisher Scientific (Roskilde, Denmark). .. Glycerol, Tris-HCl and thin layer chromatography plates were from Merck (Darmstadt, Germany).

    Modification:

    Article Title: Tumor Infiltrating Lymphocytes Genetically Engineered with an Inducible Gene Encoding Interleukin-12 for the Immunotherapy of Metastatic Melanoma
    Article Snippet: After transduction, cells were transferred from the 6 well plates into 175cm2 Nunc Cell Culture Treated Flasks with Filter Caps (Thermo Scientific) at a concentration of 0.5×106 cells/ml. .. Because IL-12 can inhibit lymphocyte proliferation, our usual procedure for lymphocyte expansion was modified.

    Incubation:

    Article Title: Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica
    Article Snippet: The plates were incubated for 7 days at 32 °C and observation of the cytopathic effect was done daily using an inverted optical microscope. .. The contents of wells with cytopathic effect were collected and inoculated in a new 25 cm2 Nunc™ Cell Culture Treated Flasks with Filter Caps (Thermo Fisher Scientific, USA) culture containing 1 million cells, the cytopathic effect was confirmed and this culture was centrifuged 10,000 rpm for 10 min (Centrifuge Sigma 1–14) for lysate clearance and were further analyzed for giant viruses.

    Article Title: Development and characterization of two cell lines from gills of Atlantic salmon
    Article Snippet: The cells were washed three times with PBS and incubated with 1 ml of trypsin while inspected under the microscope. .. After passage 4, the cells were transferred to and cultivated in Nunc™ cell culture-treated EasYFlasks™ (Thermo Scientific, US) and kept at 20°C.

    Article Title: Therapeutic Benefits of Young, But Not Old, Adipose-Derived Mesenchymal Stem Cells in a Chronic Mouse Model of Bleomycin-Induced Pulmonary Fibrosis
    Article Snippet: Cells were expanded in plastic Thermo Scientific™ Nunc™ Cell Culture Treated Flasks with Filter Caps (Thermo Fisher Scientific, Inc., Waltham, MA). .. After a 24-hr incubation period, non-adherent cells were removed.

    Thin Layer Chromatography:

    Article Title: Utilization of lactic acid in human myotubes and interplay with glucose and fatty acid metabolism
    Article Snippet: Nunc™ Cell Culture Treated Flasks with Filter Caps, Nunc™ 96-MicroWell™ plates, TaqMan reverse transcription kit reagents, High-Capacity cDNA Reverse Transcription Kit, primers for TaqMan PCR, MicroAmp® Optical 96-well Reaction Plate, MicroAmp® Optical Adhesive Film, and Power SYBR® Green PCR Master Mix were obtained from ThermoFisher Scientific (Roskilde, Denmark). .. Glycerol, Tris-HCl and thin layer chromatography plates were from Merck (Darmstadt, Germany).

    Co-Culture Assay:

    Article Title: Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica
    Article Snippet: The well contents were then collected, frozen and thawed three times to lyse the bacterial and fungal cells that may be present in the samples and thereby decrease the chance of co-culture contamination and also helps release the viruses of amoeba cells not lysed. .. The contents of wells with cytopathic effect were collected and inoculated in a new 25 cm2 Nunc™ Cell Culture Treated Flasks with Filter Caps (Thermo Fisher Scientific, USA) culture containing 1 million cells, the cytopathic effect was confirmed and this culture was centrifuged 10,000 rpm for 10 min (Centrifuge Sigma 1–14) for lysate clearance and were further analyzed for giant viruses.

    Article Title: Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica
    Article Snippet: Amoeba were grown in 75 cm2 Nunc™ Cell Culture Treated Flasks with Filter Caps (Thermo Fisher Scientific, USA) with 30 mL of peptone-yeast extract-glucose (PYG) medium supplemented with 0,14 mg/mL penicillin (Sigma-Aldrich, USA), 50 mg/mL gentamycin (Thermo Fisher Scientific, USA) and 2.5 mg/mL amphotericin (Bristol-Myers- Squibb, New York, USA) at 32 °C. .. For co-culture, amoeba were re-suspended in 10 mL of PYG supplemented with an antibiotic mix containing 0,004 mg/mL ciprofloxacin (Cellofarm, Brazil), 0,004 mg/mL vancomycin (Sigma-Aldrich, U.S.A), and 0,020 mg/mL doxycycline (Sigma-Aldrich, U.S.A).

    Expressing:

    Article Title: Optimizing assembly and production of native bispecific antibodies by codon de-optimization
    Article Snippet: Paragraph title: Transient IgG expression in PEAK cells ... One day before transfection, confluent cells were split 1:4 in T175 flasks (Nunc™ Cell Culture Treated Flasks with Filter Caps, Thermo Fisher Scientific) to have cells in the exponential growth phase.

    Article Title: Tracing information flow from Erk to target gene induction reveals mechanisms of dynamic and combinatorial control
    Article Snippet: Cells were maintained on Thermo Scientific™ Nunc™ Cell Culture Treated Flasks with Filter Caps and grown at 37°C with 5% CO2 . .. For cell lines expressing puromycin and/or hygromycin resistance genes media was supplemented with 50 μg/ml hygromycin and/or 1 μg/ml puromycin.

    Article Title: Tuning Relative Polypeptide Expression to Optimize Assembly, Yield and Downstream Processing of Bispecific Antibodies
    Article Snippet: Paragraph title: 2.3. Transient IgG Expression in Mammalian Cells ... One day before transfection, confluent cells were splited 1:4 in T175 flasks (Nunc™ Cell Culture Treated Flasks with Filter Caps, Thermo Scientific, Waltham, MA, USA) in order to have cells in the exponential growth phase.

    Protease Inhibitor:

    Article Title: Utilization of lactic acid in human myotubes and interplay with glucose and fatty acid metabolism
    Article Snippet: Bovine serum albumin (BSA, essentially fatty acid free), L-carnitine, D-glucose, oleic acid (OA, 18:1, n-9), palmitic acid (PA, 16:0), sodium lactic acid, trypan blue 0.4% solution, HEPES, DMSO, L-glutamine, gentamicin, cytochalasin B, α-cyano-4-hydroxycinnamate (CHC), protease inhibitor, phosphatase II inhibitor, β-mercaptoethanol, and Ponceau S solution were from Sigma-Aldrich (St.Louis, MO, US). .. Nunc™ Cell Culture Treated Flasks with Filter Caps, Nunc™ 96-MicroWell™ plates, TaqMan reverse transcription kit reagents, High-Capacity cDNA Reverse Transcription Kit, primers for TaqMan PCR, MicroAmp® Optical 96-well Reaction Plate, MicroAmp® Optical Adhesive Film, and Power SYBR® Green PCR Master Mix were obtained from ThermoFisher Scientific (Roskilde, Denmark).

    Polymerase Chain Reaction:

    Article Title: Utilization of lactic acid in human myotubes and interplay with glucose and fatty acid metabolism
    Article Snippet: .. Nunc™ Cell Culture Treated Flasks with Filter Caps, Nunc™ 96-MicroWell™ plates, TaqMan reverse transcription kit reagents, High-Capacity cDNA Reverse Transcription Kit, primers for TaqMan PCR, MicroAmp® Optical 96-well Reaction Plate, MicroAmp® Optical Adhesive Film, and Power SYBR® Green PCR Master Mix were obtained from ThermoFisher Scientific (Roskilde, Denmark). .. Glycerol, Tris-HCl and thin layer chromatography plates were from Merck (Darmstadt, Germany).

    Western Blot:

    Article Title: Utilization of lactic acid in human myotubes and interplay with glucose and fatty acid metabolism
    Article Snippet: Nunc™ Cell Culture Treated Flasks with Filter Caps, Nunc™ 96-MicroWell™ plates, TaqMan reverse transcription kit reagents, High-Capacity cDNA Reverse Transcription Kit, primers for TaqMan PCR, MicroAmp® Optical 96-well Reaction Plate, MicroAmp® Optical Adhesive Film, and Power SYBR® Green PCR Master Mix were obtained from ThermoFisher Scientific (Roskilde, Denmark). .. Bio Rad Protein Assay Dye Reagent Concentrate, Clarity™ Western ECL Substrate, Tris/glycine buffer, Tris/glycine/SDS buffer, SDS, Tween 20, brom-phenyl blue, Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (170–6515) secondary antibody, Goat Anti-Mouse IgG (H + L)-HRP Conjugate (170–6516) secondary antibody, and Mini-Protean® TGX™ gels (4–20%) were from Bio-Rad (Copenhagen, Denmark).

    Transformation Assay:

    Article Title: Optimizing assembly and production of native bispecific antibodies by codon de-optimization
    Article Snippet: Transformed Human Embryo Kidney monolayer epithelial cells (PEAK cells; Edge Bio) were maintained in 5% CO2 at 37°C in a humidified atmosphere in DMEM (Thermo Fisher Scientific, Waltham, MA) containing 10% FCS (Sigma-Aldrich, St Louis, MO) and supplemented with 2mM glutamine (Sigma-Aldrich), referring to complete DMEM. .. One day before transfection, confluent cells were split 1:4 in T175 flasks (Nunc™ Cell Culture Treated Flasks with Filter Caps, Thermo Fisher Scientific) to have cells in the exponential growth phase.

    Article Title: Tuning Relative Polypeptide Expression to Optimize Assembly, Yield and Downstream Processing of Bispecific Antibodies
    Article Snippet: Transient IgG Expression in Mammalian Cells Transformed Human Embryo Kidney monolayer epithelial cells (PEAK cells; Edge Bio, La Jolla, CA, USA) were maintained in 5% CO2 at 37 °C in a humidified atmosphere in DMEM (Invitrogen, Carlsbad, CA, USA) containing 10% FCS (Sigma-Aldrich, St Louis, MO, USA) and supplemented with 2 mM glutamine (Sigma-Aldrich, St Louis, MO, USA), referring to complete DMEM. .. One day before transfection, confluent cells were splited 1:4 in T175 flasks (Nunc™ Cell Culture Treated Flasks with Filter Caps, Thermo Scientific, Waltham, MA, USA) in order to have cells in the exponential growth phase.

    Injection:

    Article Title: Therapeutic Benefits of Young, But Not Old, Adipose-Derived Mesenchymal Stem Cells in a Chronic Mouse Model of Bleomycin-Induced Pulmonary Fibrosis
    Article Snippet: Mice were anesthetized by a ketamine (200 mg/kg) and xylazine (10 mg/kg) injected intraperitoneally. .. Cells were expanded in plastic Thermo Scientific™ Nunc™ Cell Culture Treated Flasks with Filter Caps (Thermo Fisher Scientific, Inc., Waltham, MA).

    Microscopy:

    Article Title: Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica
    Article Snippet: The plates were incubated for 7 days at 32 °C and observation of the cytopathic effect was done daily using an inverted optical microscope. .. The contents of wells with cytopathic effect were collected and inoculated in a new 25 cm2 Nunc™ Cell Culture Treated Flasks with Filter Caps (Thermo Fisher Scientific, USA) culture containing 1 million cells, the cytopathic effect was confirmed and this culture was centrifuged 10,000 rpm for 10 min (Centrifuge Sigma 1–14) for lysate clearance and were further analyzed for giant viruses.

    Article Title: Development and characterization of two cell lines from gills of Atlantic salmon
    Article Snippet: The cells were washed three times with PBS and incubated with 1 ml of trypsin while inspected under the microscope. .. After passage 4, the cells were transferred to and cultivated in Nunc™ cell culture-treated EasYFlasks™ (Thermo Scientific, US) and kept at 20°C.

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