Structured Review

GATC Biotech nucleotide sequences
Nucleotide Sequences, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nucleotide sequences/product/GATC Biotech
Average 92 stars, based on 7 article reviews
Price from $9.99 to $1999.99
nucleotide sequences - by Bioz Stars, 2020-09
92/100 stars

Images

Related Articles

Isolation:

Article Title: Maltose Uptake by the Novel ABC Transport System MusEFGK2I Causes Increased Expression of ptsG in Corynebacterium glutamicum
Article Snippet: .. The resulting plasmids were isolated and the nucleotide sequences controlled by sequencing (GATC Biotech). .. The promoter probe vector pET2 was used to construct a transcriptional fusion of the musF (cg2704) promoter to the promoterless cat gene.

Article Title: Production of the compatible solute α-d-glucosylglycerol by metabolically engineered Corynebacterium glutamicum
Article Snippet: .. The resulting plasmid pEKEx2- ggpSP was isolated from E. coli and the nucleotide sequence controlled by sequencing (GATC Biotech). .. For heterologous expression of ggpS and ggpP in kanamycin-resistant C. glutamicum integration mutants the plasmid pEKEx3- ggpSP was constructed.

Construct:

Article Title: A lipid-binding loop of botulinum neurotoxin serotypes B, DC and G is an essential feature to confer their exquisite potency
Article Snippet: .. Nucleotide sequences of all newly generated constructs were verified by DNA sequencing (GATC Biotech, Konstanz, Germany). .. Expression and purification of recombinant proteins In general, expression and purification of active full-length BoNT and mutants thereof were conducted under biosafety level 2 containment (project number GAA A/Z 40654/3/123).

Article Title: A Reverse Genetics System for Zika Virus Based on a Simple Molecular Cloning Strategy
Article Snippet: .. All nucleotide sequences of the final constructs were validated by using Sanger sequencing (GATC Biotech). .. In Vitro Transcription and RNA Transfection The protocol for in vitro transcription has been described earlier [ ].

Sequencing:

Article Title: Establishment of a novel anabolism-based addiction system with an artificially introduced mevalonate pathway: complete stabilization of plasmids as universal application in white biotechnology.
Article Snippet: .. Plasmid stability in recombinant microorganisms is a very important requirement for highly efficient plasmid-based production processes in biotechnology. ..

Article Title: Maltose Uptake by the Novel ABC Transport System MusEFGK2I Causes Increased Expression of ptsG in Corynebacterium glutamicum
Article Snippet: .. The resulting plasmids were isolated and the nucleotide sequences controlled by sequencing (GATC Biotech). .. The promoter probe vector pET2 was used to construct a transcriptional fusion of the musF (cg2704) promoter to the promoterless cat gene.

Article Title: Epigenetic changes around the pX region and spontaneous HTLV-1 transcription are CTCF-independent
Article Snippet: .. The DNA sequence of the putative mutants was confirmed by Sanger sequencing (GATC Biotech). .. HTLV-1-infected T cell clones were subjected to single-molecule RNA-FISH, targeting the plus- or minus-strand transcripts of HTLV-1, following the protocol described previously .

Article Title: Neisseria gonorrhoeae PilV, a type IV pilus-associated protein essential to human epithelial cell adherence
Article Snippet: .. The nucleotide sequence of pilV and pilE alleles was determined directly from PCR products at GATC Biotech AG (Konstanz, Germany). .. A pilV insertion mutant was constructed by cloning a kanamycin resistance gene cassette into the unique Xho I site at codons L +4/E +5 of PilV in pPilV1 to yield pPILV2.

Article Title: Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered Pseudomonas putida KT2440
Article Snippet: .. The nucleotide sequence of xylAB was analyzed and confirmed by GATC Biotech AG (Konstanz, Germany). .. Introduction of pSLM1 into P. putida KT2440 The obtained plasmid pSLM1 was introduced into P. putida KT2440 by triparental mating [ ].

Article Title: Production of the compatible solute α-d-glucosylglycerol by metabolically engineered Corynebacterium glutamicum
Article Snippet: .. The resulting plasmid pEKEx2- ggpSP was isolated from E. coli and the nucleotide sequence controlled by sequencing (GATC Biotech). .. For heterologous expression of ggpS and ggpP in kanamycin-resistant C. glutamicum integration mutants the plasmid pEKEx3- ggpSP was constructed.

Article Title: A Reverse Genetics System for Zika Virus Based on a Simple Molecular Cloning Strategy
Article Snippet: .. All nucleotide sequences of the final constructs were validated by using Sanger sequencing (GATC Biotech). .. In Vitro Transcription and RNA Transfection The protocol for in vitro transcription has been described earlier [ ].

Generated:

Article Title: A lipid-binding loop of botulinum neurotoxin serotypes B, DC and G is an essential feature to confer their exquisite potency
Article Snippet: .. Nucleotide sequences of all newly generated constructs were verified by DNA sequencing (GATC Biotech, Konstanz, Germany). .. Expression and purification of recombinant proteins In general, expression and purification of active full-length BoNT and mutants thereof were conducted under biosafety level 2 containment (project number GAA A/Z 40654/3/123).

DNA Sequencing:

Article Title: A lipid-binding loop of botulinum neurotoxin serotypes B, DC and G is an essential feature to confer their exquisite potency
Article Snippet: .. Nucleotide sequences of all newly generated constructs were verified by DNA sequencing (GATC Biotech, Konstanz, Germany). .. Expression and purification of recombinant proteins In general, expression and purification of active full-length BoNT and mutants thereof were conducted under biosafety level 2 containment (project number GAA A/Z 40654/3/123).

Polymerase Chain Reaction:

Article Title: Neisseria gonorrhoeae PilV, a type IV pilus-associated protein essential to human epithelial cell adherence
Article Snippet: .. The nucleotide sequence of pilV and pilE alleles was determined directly from PCR products at GATC Biotech AG (Konstanz, Germany). .. A pilV insertion mutant was constructed by cloning a kanamycin resistance gene cassette into the unique Xho I site at codons L +4/E +5 of PilV in pPilV1 to yield pPILV2.

Plasmid Preparation:

Article Title: Establishment of a novel anabolism-based addiction system with an artificially introduced mevalonate pathway: complete stabilization of plasmids as universal application in white biotechnology.
Article Snippet: .. Plasmid stability in recombinant microorganisms is a very important requirement for highly efficient plasmid-based production processes in biotechnology. ..

Article Title: Production of the compatible solute α-d-glucosylglycerol by metabolically engineered Corynebacterium glutamicum
Article Snippet: .. The resulting plasmid pEKEx2- ggpSP was isolated from E. coli and the nucleotide sequence controlled by sequencing (GATC Biotech). .. For heterologous expression of ggpS and ggpP in kanamycin-resistant C. glutamicum integration mutants the plasmid pEKEx3- ggpSP was constructed.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    GATC Biotech b10 riii control mice
    Graphical presentation of the Med13L protein and amino acid-altering SNPs between <t>B10.RIII</t> and BR.RIIIS/J- Eae39r1 mice. The conserved N terminal (Med13l_N) and C terminal (Med13l_C) domains are shown in grey. Numbers representing amino acid residue and location of amino acid changes due to coding non-synonymous SNPs between B10.RIII and BR.RIIIS/J- Eae39r1 mice (E814D and V1222I) are shown by arrows
    B10 Riii Control Mice, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b10 riii control mice/product/GATC Biotech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    b10 riii control mice - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    GATC Biotech cdna sequencing
    Comparison of 152 transcript levels (40 downregulated in CDM and 112 upregulated in CDM) that were consistently classified among the DEG gene sets as determined by <t>microarray</t> transcriptomes or direct <t>cDNA</t> and 3′-UTR transcriptome sequencing. Data
    Cdna Sequencing, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna sequencing/product/GATC Biotech
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    cdna sequencing - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    GATC Biotech cdna library construction
    Overview of <t>RNA</t> sample preparation. Whole brains were dissected from isofemale lines derived from a European (the Netherlands) and an African (Zimbabwe) population. Brains were pooled within each population for RNA extraction. The dissection and pooling procedures were performed for two biological replicates of each population and sex, resulting in a total of eight samples that were used for <t>cDNA</t> library construction and high-throughput sequencing.
    Cdna Library Construction, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna library construction/product/GATC Biotech
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    cdna library construction - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    90
    GATC Biotech unique rflp patterns
    The <t>cytb-qPCR</t> products and <t>RFLP</t> assays for species determination. a) The 430 bp cytb-qPCR products for each species. b-e) Four RFLP assays using FspBI, AluI, HpyCH4V and Csp6I restriction enzymes for Plasmodium species determination. f) Schematic diagram showing the RFLP product lengths. a-e) Lane 1, GeneRuler low range DNA ladder (Thermo Fisher); F, P . falciparum ; V, P . vivax ; M, P . malariae ; O, P . ovale ; NC, negative control.
    Unique Rflp Patterns, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unique rflp patterns/product/GATC Biotech
    Average 90 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    unique rflp patterns - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Graphical presentation of the Med13L protein and amino acid-altering SNPs between B10.RIII and BR.RIIIS/J- Eae39r1 mice. The conserved N terminal (Med13l_N) and C terminal (Med13l_C) domains are shown in grey. Numbers representing amino acid residue and location of amino acid changes due to coding non-synonymous SNPs between B10.RIII and BR.RIIIS/J- Eae39r1 mice (E814D and V1222I) are shown by arrows

    Journal: Archivum Immunologiae et Therapiae Experimentalis

    Article Title: Analysis of Polymorphisms in the Mediator Complex Subunit 13-like (Med13L) Gene in the Context of Immune Function and Development of Experimental Arthritis

    doi: 10.1007/s00005-018-0516-8

    Figure Lengend Snippet: Graphical presentation of the Med13L protein and amino acid-altering SNPs between B10.RIII and BR.RIIIS/J- Eae39r1 mice. The conserved N terminal (Med13l_N) and C terminal (Med13l_C) domains are shown in grey. Numbers representing amino acid residue and location of amino acid changes due to coding non-synonymous SNPs between B10.RIII and BR.RIIIS/J- Eae39r1 mice (E814D and V1222I) are shown by arrows

    Article Snippet: For DNA sequencing, all coding exons (and surrounding regions) of the Med13L gene for BR.RIIIS/J-Eae39r1 congenic and B10.RIII control mice were amplified with specific primer pairs and sequenced by Sanger technology (GATC biotech, Germany).

    Techniques: Mouse Assay

    Collagen-induced arthritis (CIA) development and progression in B10.RIIIS/J -Eae39r1 mice in comparison to B10.RIII littermate control mice, represented as mean arthritis score for each group ± standard error of the mean (SEM) ( a ); IgM ( b ) and IgG ( c ) anti-collagen type II antibody responses in early pre-clinical CIA, measured on day 15, and late clinical CIA, measured on day 72. ( n = 9 for B10.RIIIS/J -Eae39r1 and n = 11 for B10.RIII)

    Journal: Archivum Immunologiae et Therapiae Experimentalis

    Article Title: Analysis of Polymorphisms in the Mediator Complex Subunit 13-like (Med13L) Gene in the Context of Immune Function and Development of Experimental Arthritis

    doi: 10.1007/s00005-018-0516-8

    Figure Lengend Snippet: Collagen-induced arthritis (CIA) development and progression in B10.RIIIS/J -Eae39r1 mice in comparison to B10.RIII littermate control mice, represented as mean arthritis score for each group ± standard error of the mean (SEM) ( a ); IgM ( b ) and IgG ( c ) anti-collagen type II antibody responses in early pre-clinical CIA, measured on day 15, and late clinical CIA, measured on day 72. ( n = 9 for B10.RIIIS/J -Eae39r1 and n = 11 for B10.RIII)

    Article Snippet: For DNA sequencing, all coding exons (and surrounding regions) of the Med13L gene for BR.RIIIS/J-Eae39r1 congenic and B10.RIII control mice were amplified with specific primer pairs and sequenced by Sanger technology (GATC biotech, Germany).

    Techniques: Mouse Assay

    In vitro proliferative responses of splenic B and T lymphocytes from B10.RIII and BR.RIIIS/J- Eae39r1 mice. 3 H-thymidine incorporation, represented as counts per minute ± SEM, of B cells following in vitro stimulation with: ( a ) titrated concentrations (0–10 µg/ml) of LPS; ( b ) titrated concentrations (0–60 µg/ml) of anti-IgM antibody. 3 H-thymidine incorporation of CD4 + T cells following in vitro stimulation with: ( c ) titrated concentrations (0–1.25 µg/ml) of ConA; ( d ) a combination of titrated concentrations (0–3 µg/ml) of anti-CD3 antibody and fixed concentration of anti-CD28 antibody (3 µg/ml). ( e, f ) IL-2 production (mean ± SEM) in the supernatant of T cells stimulated with ConA or anti-CD3/CD28 antibodies

    Journal: Archivum Immunologiae et Therapiae Experimentalis

    Article Title: Analysis of Polymorphisms in the Mediator Complex Subunit 13-like (Med13L) Gene in the Context of Immune Function and Development of Experimental Arthritis

    doi: 10.1007/s00005-018-0516-8

    Figure Lengend Snippet: In vitro proliferative responses of splenic B and T lymphocytes from B10.RIII and BR.RIIIS/J- Eae39r1 mice. 3 H-thymidine incorporation, represented as counts per minute ± SEM, of B cells following in vitro stimulation with: ( a ) titrated concentrations (0–10 µg/ml) of LPS; ( b ) titrated concentrations (0–60 µg/ml) of anti-IgM antibody. 3 H-thymidine incorporation of CD4 + T cells following in vitro stimulation with: ( c ) titrated concentrations (0–1.25 µg/ml) of ConA; ( d ) a combination of titrated concentrations (0–3 µg/ml) of anti-CD3 antibody and fixed concentration of anti-CD28 antibody (3 µg/ml). ( e, f ) IL-2 production (mean ± SEM) in the supernatant of T cells stimulated with ConA or anti-CD3/CD28 antibodies

    Article Snippet: For DNA sequencing, all coding exons (and surrounding regions) of the Med13L gene for BR.RIIIS/J-Eae39r1 congenic and B10.RIII control mice were amplified with specific primer pairs and sequenced by Sanger technology (GATC biotech, Germany).

    Techniques: In Vitro, Mouse Assay, Concentration Assay

    Comparison of 152 transcript levels (40 downregulated in CDM and 112 upregulated in CDM) that were consistently classified among the DEG gene sets as determined by microarray transcriptomes or direct cDNA and 3′-UTR transcriptome sequencing. Data

    Journal: Applied and Environmental Microbiology

    Article Title: Comparative Analysis of Lactobacillus plantarum WCFS1 Transcriptomes by Using DNA Microarray and Next-Generation Sequencing Technologies

    doi: 10.1128/AEM.00470-12

    Figure Lengend Snippet: Comparison of 152 transcript levels (40 downregulated in CDM and 112 upregulated in CDM) that were consistently classified among the DEG gene sets as determined by microarray transcriptomes or direct cDNA and 3′-UTR transcriptome sequencing. Data

    Article Snippet: This is also reflected by the somewhat lower Pearson correlation for the comparison of the microarray to 3′-UTR sequencing (0.852; P < 0.01) relative to the comparison of the microarray to direct cDNA sequencing (0.897; P < 0.01).

    Techniques: Microarray, Sequencing

    Comparison between normalized signal intensity level of microarray and normalized read counts of direct cDNA sequencing (A and B) and 3′-UTR sequencing (C and D) in transcriptome data sets from bacteria grown in CDM and MRS.

    Journal: Applied and Environmental Microbiology

    Article Title: Comparative Analysis of Lactobacillus plantarum WCFS1 Transcriptomes by Using DNA Microarray and Next-Generation Sequencing Technologies

    doi: 10.1128/AEM.00470-12

    Figure Lengend Snippet: Comparison between normalized signal intensity level of microarray and normalized read counts of direct cDNA sequencing (A and B) and 3′-UTR sequencing (C and D) in transcriptome data sets from bacteria grown in CDM and MRS.

    Article Snippet: This is also reflected by the somewhat lower Pearson correlation for the comparison of the microarray to 3′-UTR sequencing (0.852; P < 0.01) relative to the comparison of the microarray to direct cDNA sequencing (0.897; P < 0.01).

    Techniques: Microarray, Sequencing

    Overview of RNA sample preparation. Whole brains were dissected from isofemale lines derived from a European (the Netherlands) and an African (Zimbabwe) population. Brains were pooled within each population for RNA extraction. The dissection and pooling procedures were performed for two biological replicates of each population and sex, resulting in a total of eight samples that were used for cDNA library construction and high-throughput sequencing.

    Journal: BMC Genomics

    Article Title: Population and sex differences in Drosophila melanogaster brain gene expression

    doi: 10.1186/1471-2164-13-654

    Figure Lengend Snippet: Overview of RNA sample preparation. Whole brains were dissected from isofemale lines derived from a European (the Netherlands) and an African (Zimbabwe) population. Brains were pooled within each population for RNA extraction. The dissection and pooling procedures were performed for two biological replicates of each population and sex, resulting in a total of eight samples that were used for cDNA library construction and high-throughput sequencing.

    Article Snippet: RNA extraction and high-throughput sequencing Total RNA extraction and DNase I digestion were performed using the MasterPure RNA Purification Kit (Epicentre). cDNA library construction and high-throughput sequencing were performed by GATC Biotech (Konstanz, Germany).

    Techniques: Sample Prep, Derivative Assay, RNA Extraction, Dissection, cDNA Library Assay, Next-Generation Sequencing

    The cytb-qPCR products and RFLP assays for species determination. a) The 430 bp cytb-qPCR products for each species. b-e) Four RFLP assays using FspBI, AluI, HpyCH4V and Csp6I restriction enzymes for Plasmodium species determination. f) Schematic diagram showing the RFLP product lengths. a-e) Lane 1, GeneRuler low range DNA ladder (Thermo Fisher); F, P . falciparum ; V, P . vivax ; M, P . malariae ; O, P . ovale ; NC, negative control.

    Journal: PLoS ONE

    Article Title: SYBR Green Real-Time PCR-RFLP Assay Targeting the Plasmodium Cytochrome B Gene – A Highly Sensitive Molecular Tool for Malaria Parasite Detection and Species Determination

    doi: 10.1371/journal.pone.0120210

    Figure Lengend Snippet: The cytb-qPCR products and RFLP assays for species determination. a) The 430 bp cytb-qPCR products for each species. b-e) Four RFLP assays using FspBI, AluI, HpyCH4V and Csp6I restriction enzymes for Plasmodium species determination. f) Schematic diagram showing the RFLP product lengths. a-e) Lane 1, GeneRuler low range DNA ladder (Thermo Fisher); F, P . falciparum ; V, P . vivax ; M, P . malariae ; O, P . ovale ; NC, negative control.

    Article Snippet: In a few cases, where the cytb-qPCR products were of correct size but showed unique RFLP patterns compared to the reference species, Plasmodium spp. was determined by DNA sequencing performed by GATC Biotech AG (Constance, Germany).

    Techniques: Real-time Polymerase Chain Reaction, Negative Control