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MACHEREY NAGEL nucleospin plant ii kit
Nucleospin Plant Ii Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nucleospin plant ii kit/product/MACHEREY NAGEL
Average 82 stars, based on 1 article reviews
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nucleospin plant ii kit - by Bioz Stars, 2020-02
82/100 stars

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Methylation Sequencing:

Article Title: Memory of plant communications for priming anti-herbivore responses
Article Snippet: .. Bisulfite sequencing of TI gene Genomic DNA was isolated from leaf tissue using a NucleoSpin Plant II Kit (MACHEREY-NAGEL, Düren, Germany) following the manufacturer's protocol. .. The genomic DNA was bisulfate treated and purified using a MethylEasy Xceed DNA Bisulphite Modification Kit (Takara, Otsu, Japan) following the manufacturer's protocol.

Amplification:

Article Title: Microbial diversity in biodeteriorated Greek historical documents dating back to the 19th and 20th century: A case study, et al. Microbial diversity in biodeteriorated Greek historical documents dating back to the 19th and 20th century: A case study
Article Snippet: .. 2.2 DNA extraction and PCR amplification from microbial isolates DNA was extracted from 17 morphologically distinct fungal isolates using the NucleoSpin Plant II Kit (Macherey‐Nagel, GERMANY) according to manufacturer's instructions. ..

Article Title: Simple sequence repeat markers for kānuka (Kunzea spp.; Myrtaceae) present in New Zealand 1
Article Snippet: Primers were designed using Primer3 , implemented in MSATCOMMANDER, with the following specifications: 80–550 bp amplicon length, repeat units flanked by ≥50 bp, and 57–62°C melting temperature ( ). .. DNA was extracted from 20 mg of dried leaf material using the NucleoSpin Plant II kit (PL1 lysis buffer; Macherey-Nagel, Düren, Germany) following manufacturer’s instructions, resulting in 200–800 ng of DNA per sample.

Article Title: Begoniayenyeniae ( Begoniaceae), a new species from Endau Rompin National Park, Johor, Malaysia
Article Snippet: Paragraph title: DNA Extraction, PCR amplification and DNA sequencing ... DNA of Begonia species was extracted from living material or silica gel dried material using the NucleoSpin Plant II kit following the manufacturer’s protocols (Macherey Nagel, Germany).

Article Title: Molecular Species Delimitation in the Racomitrium canescens Complex (Grimmiaceae) and Implications for DNA Barcoding of Species Complexes in Mosses
Article Snippet: Total genomic DNA was extracted using the DNeasy® Plant Kit (Qiagen) or the NucleoSpin® Plant II Kit (Macherey-Nagel). .. The employed molecular markers were amplified by PCR using protocols and primers as described in for rps4-trnT-trnL (primers rps4-166F and P6/7) and for nrITS (primers 18F and 25R).

Construct:

Article Title: Characterisation of pathogen-specific regions and novel effector candidates in Fusarium oxysporum f. sp. cepae
Article Snippet: DNA was extracted from freeze-dried mycelium for the three Foc (Fus2, 125, A23) and four Fo isolates (A13, A28, PG, CB3) using the Macherey-Nagel Nucleospin Plant II kit (Fisher 11912262). .. Illumina libraries were constructed using either Illumina TruSeq LT kit (FC-121-2001), or with a PCR-free method using NEBNext End Repair (E6050S), NEBNext dA-tailing (E6053S) and Blunt T/A ligase (M0367S) New England Biolabs modules.

Real-time Polymerase Chain Reaction:

Article Title: Spatio-Temporal Dynamic of Tuber magnatum Mycelium in Natural Truffle Grounds
Article Snippet: Paragraph title: DNA extraction and qPCR assay ... Crude DNA solutions were then purified using the Nucleospin Plant II kit (Macherey-Nagel).

Mass Spectrometry:

Article Title: Characterisation of pathogen-specific regions and novel effector candidates in Fusarium oxysporum f. sp. cepae
Article Snippet: DNA was extracted from freeze-dried mycelium for the three Foc (Fus2, 125, A23) and four Fo isolates (A13, A28, PG, CB3) using the Macherey-Nagel Nucleospin Plant II kit (Fisher 11912262). .. Libraries were sequenced using Illumina Miseq v2 2 × 250 bp PE (MS-102-2003) or v3 2 × 300 bp PE (MS-102-3003).

Modification:

Article Title: Memory of plant communications for priming anti-herbivore responses
Article Snippet: Bisulfite sequencing of TI gene Genomic DNA was isolated from leaf tissue using a NucleoSpin Plant II Kit (MACHEREY-NAGEL, Düren, Germany) following the manufacturer's protocol. .. The genomic DNA was bisulfate treated and purified using a MethylEasy Xceed DNA Bisulphite Modification Kit (Takara, Otsu, Japan) following the manufacturer's protocol.

Derivative Assay:

Article Title: Identification of QTLs conferring resistance to downy mildew in legacy cultivars of lettuce
Article Snippet: Development of mapping population and linkage map Mapping population consisted of 90 F6 RILs that were derived from a Grand Rapids × Iceberg cross using single seed descent. .. Lyophilized samples were ground to fine powder using TissueLyser mill, before extracting genomic DNA with NucleoSpin Plant II kit (Macherey-Nagel, Betlehem, PA, USA).

Northern Blot:

Article Title: Sequencing and Analysis of the Genome of the Filamentous Fungus Penicillium verrucosum BFE808, Which Produces the Mycotoxins Citrinin and Ochratoxin
Article Snippet: P. verrucosum is psychrotolerant and occurs mainly in moderate-temperature regions, such as Northern Europe, North America, Canada, and parts of South America. .. DNA was extracted using the NucleoSpin Plant II kit (Macherey-Nagel, Germany) and was quantified and quality checked using NanoDrop 1000 (VWR International, Germany) and the Qubit 3.0 photometer, respectively.

Generated:

Article Title: Molecular Species Delimitation in the Racomitrium canescens Complex (Grimmiaceae) and Implications for DNA Barcoding of Species Complexes in Mosses
Article Snippet: Sequences from the plastid rps4-trnT-trnL and nuclear ribosomal ITS regions were newly generated for 70 specimens of the Racomitrium canescens complex. .. Total genomic DNA was extracted using the DNeasy® Plant Kit (Qiagen) or the NucleoSpin® Plant II Kit (Macherey-Nagel).

DNA Sequencing:

Article Title: Begoniayenyeniae ( Begoniaceae), a new species from Endau Rompin National Park, Johor, Malaysia
Article Snippet: Paragraph title: DNA Extraction, PCR amplification and DNA sequencing ... DNA of Begonia species was extracted from living material or silica gel dried material using the NucleoSpin Plant II kit following the manufacturer’s protocols (Macherey Nagel, Germany).

Sequencing:

Article Title: Draft Genome Sequence of Mycobacterium sp. Strain shizuoka-1, a Novel Mycobacterium Isolated from Groundwater of a Bathing Facility in Shizuoka, Japan
Article Snippet: Here, we report the draft genome sequence of this mycobacterium, named Mycobacterium sp. strain shizuoka-1. .. DNA was extracted using the NucleoSpin plant II kit (Macherey-Nagel, Düren, Germany).

Article Title: Simple sequence repeat markers for kānuka (Kunzea spp.; Myrtaceae) present in New Zealand 1
Article Snippet: From a total of 3174 putative simple sequence repeat regions, 96 primer pairs, providing a range of product sizes and repeat units, were screened. .. DNA was extracted from 20 mg of dried leaf material using the NucleoSpin Plant II kit (PL1 lysis buffer; Macherey-Nagel, Düren, Germany) following manufacturer’s instructions, resulting in 200–800 ng of DNA per sample.

Article Title: Identification of fasciclin-like arabinogalactan proteins in textile hemp (Cannabis sativa L.): in silico analyses and gene expression patterns in different tissues
Article Snippet: Paragraph title: Sequencing of some representative CsaFLA promoters ... Genomic DNA was extracted from stem tissues (whole internodes) by using a CTAB-based protocol coupled to the NucleoSpin Plant II kit (Macherey-Nagel).

Article Title: Sequencing and Analysis of the Genome of the Filamentous Fungus Penicillium verrucosum BFE808, Which Produces the Mycotoxins Citrinin and Ochratoxin
Article Snippet: Genome sequencing was done on the MiSeq platform (Illumina, USA) after P. verrucosum BFE808 was grown for 7 days at 25°C in yeast extract-sucrose (YES) broth (20 g/liter yeast extract, 150 g/liter saccharose). .. DNA was extracted using the NucleoSpin Plant II kit (Macherey-Nagel, Germany) and was quantified and quality checked using NanoDrop 1000 (VWR International, Germany) and the Qubit 3.0 photometer, respectively.

Article Title: Molecular Species Delimitation in the Racomitrium canescens Complex (Grimmiaceae) and Implications for DNA Barcoding of Species Complexes in Mosses
Article Snippet: Paragraph title: Taxon sampling, DNA extraction, PCR and sequencing ... Total genomic DNA was extracted using the DNeasy® Plant Kit (Qiagen) or the NucleoSpin® Plant II Kit (Macherey-Nagel).

Article Title: Characterisation of pathogen-specific regions and novel effector candidates in Fusarium oxysporum f. sp. cepae
Article Snippet: Paragraph title: DNA extraction, library preparation and sequencing ... DNA was extracted from freeze-dried mycelium for the three Foc (Fus2, 125, A23) and four Fo isolates (A13, A28, PG, CB3) using the Macherey-Nagel Nucleospin Plant II kit (Fisher 11912262).

DNA Extraction:

Article Title: Spatio-Temporal Dynamic of Tuber magnatum Mycelium in Natural Truffle Grounds
Article Snippet: Paragraph title: DNA extraction and qPCR assay ... Crude DNA solutions were then purified using the Nucleospin Plant II kit (Macherey-Nagel).

Article Title: Microbial diversity in biodeteriorated Greek historical documents dating back to the 19th and 20th century: A case study, et al. Microbial diversity in biodeteriorated Greek historical documents dating back to the 19th and 20th century: A case study
Article Snippet: .. 2.2 DNA extraction and PCR amplification from microbial isolates DNA was extracted from 17 morphologically distinct fungal isolates using the NucleoSpin Plant II Kit (Macherey‐Nagel, GERMANY) according to manufacturer's instructions. ..

Article Title: Identification of QTLs conferring resistance to downy mildew in legacy cultivars of lettuce
Article Snippet: Tissue for DNA extraction was obtained from young leaves of about one month old plants and immediately lyophilized. .. Lyophilized samples were ground to fine powder using TissueLyser mill, before extracting genomic DNA with NucleoSpin Plant II kit (Macherey-Nagel, Betlehem, PA, USA).

Article Title: Begoniayenyeniae ( Begoniaceae), a new species from Endau Rompin National Park, Johor, Malaysia
Article Snippet: Paragraph title: DNA Extraction, PCR amplification and DNA sequencing ... DNA of Begonia species was extracted from living material or silica gel dried material using the NucleoSpin Plant II kit following the manufacturer’s protocols (Macherey Nagel, Germany).

Article Title: Molecular Species Delimitation in the Racomitrium canescens Complex (Grimmiaceae) and Implications for DNA Barcoding of Species Complexes in Mosses
Article Snippet: Paragraph title: Taxon sampling, DNA extraction, PCR and sequencing ... Total genomic DNA was extracted using the DNeasy® Plant Kit (Qiagen) or the NucleoSpin® Plant II Kit (Macherey-Nagel).

Article Title: Characterisation of pathogen-specific regions and novel effector candidates in Fusarium oxysporum f. sp. cepae
Article Snippet: Paragraph title: DNA extraction, library preparation and sequencing ... DNA was extracted from freeze-dried mycelium for the three Foc (Fus2, 125, A23) and four Fo isolates (A13, A28, PG, CB3) using the Macherey-Nagel Nucleospin Plant II kit (Fisher 11912262).

Nucleic Acid Electrophoresis:

Article Title: Identification of QTLs conferring resistance to downy mildew in legacy cultivars of lettuce
Article Snippet: Lyophilized samples were ground to fine powder using TissueLyser mill, before extracting genomic DNA with NucleoSpin Plant II kit (Macherey-Nagel, Betlehem, PA, USA). .. Concentration and quality of the DNA was analyzed with an ND-1000 Spectrometer (NanoDrop Technologies, Wilmington, DE, USA) and gel electrophoresis.

Isolation:

Article Title: Spatio-Temporal Dynamic of Tuber magnatum Mycelium in Natural Truffle Grounds
Article Snippet: DNA extraction and qPCR assay DNAs were isolated using a CTAB-based buffer (2% CTAB, 2% Polyvinylpyrrolidon, 2 M NaCl, 20 mM EDTA, 100 mM Tris–HCl, pH 8), following the protocol described by Iotti et al. . .. Crude DNA solutions were then purified using the Nucleospin Plant II kit (Macherey-Nagel).

Article Title: Memory of plant communications for priming anti-herbivore responses
Article Snippet: .. Bisulfite sequencing of TI gene Genomic DNA was isolated from leaf tissue using a NucleoSpin Plant II Kit (MACHEREY-NAGEL, Düren, Germany) following the manufacturer's protocol. .. The genomic DNA was bisulfate treated and purified using a MethylEasy Xceed DNA Bisulphite Modification Kit (Takara, Otsu, Japan) following the manufacturer's protocol.

Article Title: Nonsense Mutation Inside Anthocyanidin Synthase Gene Controls Pigmentation in Yellow Raspberry (Rubus idaeus L.)
Article Snippet: DNA and RNA Extraction Total RNA was isolated from leaves and different fruit stages using Spectrum Plant Total RNA kit (Sigma, Germany) according to the manufacturer’s instructions. .. Total genomic DNA was also extracted from young and old leaves according to instructions of NucleoSpin® Plant II kit (Macherey-Nagel, Düren, Germany).

Article Title: Draft Genome Sequence of Mycobacterium sp. Strain shizuoka-1, a Novel Mycobacterium Isolated from Groundwater of a Bathing Facility in Shizuoka, Japan
Article Snippet: This time, we isolated a novel rapidly growing scotochromogenic mycobacterium from well water for a hot bathing facility in Shizuoka prefecture, Japan. .. DNA was extracted using the NucleoSpin plant II kit (Macherey-Nagel, Düren, Germany).

Article Title: Functional characterization of squalene synthase and squalene epoxidase in Taraxacum koksaghyz, et al. Functional characterization of squalene synthase and squalene epoxidase in Taraxacum koksaghyz
Article Snippet: .. 2.6 Isolation of genomic DNA Genomic DNA was isolated using the NucleoSpin Plant II Kit (Macherey‐Nagel, Düren, Germany) according to the manufacturer's instructions. .. 2.7 Cloning and stable transformation procedures For localization studies, the full‐length TkSQS1 cDNA was amplified using primers TkSQS1‐fwd‐blunt and TkSQS1‐rev‐XhoI, digested with XhoI and inserted into the XmnI/XhoI sites of the Gateway‐compatible vector pENTR3C (Thermo Fisher Scientific Inc., Darmstadt, Germany), resulting in the final vector pENTR3C‐TkSQS1.

Article Title: Sequencing and Analysis of the Genome of the Filamentous Fungus Penicillium verrucosum BFE808, Which Produces the Mycotoxins Citrinin and Ochratoxin
Article Snippet: The strain P. verrucosum BFE808, whose genome sequence is announced here, was isolated from wheat kernels and produces the mycotoxins ochratoxin and citrinin , which both have detrimental effects on the kidneys, liver, and immune system ( ). .. DNA was extracted using the NucleoSpin Plant II kit (Macherey-Nagel, Germany) and was quantified and quality checked using NanoDrop 1000 (VWR International, Germany) and the Qubit 3.0 photometer, respectively.

Purification:

Article Title: Spatio-Temporal Dynamic of Tuber magnatum Mycelium in Natural Truffle Grounds
Article Snippet: .. Crude DNA solutions were then purified using the Nucleospin Plant II kit (Macherey-Nagel). .. Total DNAs were quantified by a NanoDrop ND-1000 Spectrophotometer (Thermo Scientific) and their quality evaluated with optical density (OD) 260/280 nm and 260/230 nm ratios.

Article Title: Memory of plant communications for priming anti-herbivore responses
Article Snippet: Bisulfite sequencing of TI gene Genomic DNA was isolated from leaf tissue using a NucleoSpin Plant II Kit (MACHEREY-NAGEL, Düren, Germany) following the manufacturer's protocol. .. The genomic DNA was bisulfate treated and purified using a MethylEasy Xceed DNA Bisulphite Modification Kit (Takara, Otsu, Japan) following the manufacturer's protocol.

Article Title: Molecular Species Delimitation in the Racomitrium canescens Complex (Grimmiaceae) and Implications for DNA Barcoding of Species Complexes in Mosses
Article Snippet: Total genomic DNA was extracted using the DNeasy® Plant Kit (Qiagen) or the NucleoSpin® Plant II Kit (Macherey-Nagel). .. PCR products were purified using the Wizard® DNA Clean-up kit (Promega) or by Macrogen Inc. ( www.macrogen.com ), where the automated sequencing was performed as well.

Polymerase Chain Reaction:

Article Title: Memory of plant communications for priming anti-herbivore responses
Article Snippet: Bisulfite sequencing of TI gene Genomic DNA was isolated from leaf tissue using a NucleoSpin Plant II Kit (MACHEREY-NAGEL, Düren, Germany) following the manufacturer's protocol. .. Two micrograms of the bisulfite-treated DNA solution were added to a first-round PCR mixture containing 1× PCR buffer, 2.5 mM MgCl2 , 0.3 mM dNTPs, 0.4 μM of each primer (5′-TTTTTATAGAGAGTATTATTAGATGGTTTA-3′ and 5′-ACCATCATAAAAAACATCTAACCACA-3′), and 0.625 U Takara EpiTaq HS enzyme (Takara) in a final volume of 25 μl.

Article Title: Microbial diversity in biodeteriorated Greek historical documents dating back to the 19th and 20th century: A case study, et al. Microbial diversity in biodeteriorated Greek historical documents dating back to the 19th and 20th century: A case study
Article Snippet: .. 2.2 DNA extraction and PCR amplification from microbial isolates DNA was extracted from 17 morphologically distinct fungal isolates using the NucleoSpin Plant II Kit (Macherey‐Nagel, GERMANY) according to manufacturer's instructions. ..

Article Title: Simple sequence repeat markers for kānuka (Kunzea spp.; Myrtaceae) present in New Zealand 1
Article Snippet: Adding an M13F tag (TGTAAAACGACGGCCAGT) to the 5′ end of the forward primers enabled the use of 6-FAM–labeled M13F probes in the second step of the PCR for economic genotyping ( ; ). .. DNA was extracted from 20 mg of dried leaf material using the NucleoSpin Plant II kit (PL1 lysis buffer; Macherey-Nagel, Düren, Germany) following manufacturer’s instructions, resulting in 200–800 ng of DNA per sample.

Article Title: Begoniayenyeniae ( Begoniaceae), a new species from Endau Rompin National Park, Johor, Malaysia
Article Snippet: Paragraph title: DNA Extraction, PCR amplification and DNA sequencing ... DNA of Begonia species was extracted from living material or silica gel dried material using the NucleoSpin Plant II kit following the manufacturer’s protocols (Macherey Nagel, Germany).

Article Title: Molecular Species Delimitation in the Racomitrium canescens Complex (Grimmiaceae) and Implications for DNA Barcoding of Species Complexes in Mosses
Article Snippet: Paragraph title: Taxon sampling, DNA extraction, PCR and sequencing ... Total genomic DNA was extracted using the DNeasy® Plant Kit (Qiagen) or the NucleoSpin® Plant II Kit (Macherey-Nagel).

Article Title: Characterisation of pathogen-specific regions and novel effector candidates in Fusarium oxysporum f. sp. cepae
Article Snippet: DNA was extracted from freeze-dried mycelium for the three Foc (Fus2, 125, A23) and four Fo isolates (A13, A28, PG, CB3) using the Macherey-Nagel Nucleospin Plant II kit (Fisher 11912262). .. Illumina libraries were constructed using either Illumina TruSeq LT kit (FC-121-2001), or with a PCR-free method using NEBNext End Repair (E6050S), NEBNext dA-tailing (E6053S) and Blunt T/A ligase (M0367S) New England Biolabs modules.

Staining:

Article Title: Begoniayenyeniae ( Begoniaceae), a new species from Endau Rompin National Park, Johor, Malaysia
Article Snippet: DNA of Begonia species was extracted from living material or silica gel dried material using the NucleoSpin Plant II kit following the manufacturer’s protocols (Macherey Nagel, Germany). .. The following PCR profile was used: initial denaturation at 95 °C for 5 min, followed by 40 cycles of denaturation at 95 °C for 1 min, primer annealing at 55 °C for 1 min and extension at 72 °C for 2 min; followed by a final extension step at 72 °C for 5 min. Amplicons were visualised using 1.0% agarose gel electrophoresis stained with SYBR Safe DNA Gel Stain (Invitrogen, U.S.A.).

Software:

Article Title: Advances in genotyping microsatellite markers through sequencing and consequences of scoring methods for Ceratonia siliqua (Leguminosae)Advances in genotyping microsatellite markers through sequencing and consequences of scoring methods for Ceratonia siliqua (Leguminosae)
Article Snippet: Total DNA of one individual was extracted from leaves stored with silica gel using the NucleoSpin Plant II Kit (Macherey‐Nagel Sarl, Hoerdt, France). .. A de novo assembly was then performed with the merged paired‐end reads using MIRA 4.0.1 software (Chevreux et al., ).

RNA Extraction:

Article Title: Nonsense Mutation Inside Anthocyanidin Synthase Gene Controls Pigmentation in Yellow Raspberry (Rubus idaeus L.)
Article Snippet: Paragraph title: DNA and RNA Extraction ... Total genomic DNA was also extracted from young and old leaves according to instructions of NucleoSpin® Plant II kit (Macherey-Nagel, Düren, Germany).

Selection:

Article Title: Molecular Species Delimitation in the Racomitrium canescens Complex (Grimmiaceae) and Implications for DNA Barcoding of Species Complexes in Mosses
Article Snippet: Taxon sampling, DNA extraction, PCR and sequencing The present taxon sampling and outgroup selection was based on recent molecular phylogenetic reconstructions of Racomitrium s.l., which showed that R. fasciculare and R. laevigatum (both treated as Codriophorus by ), formed a sister clade to the R. canescens complex , (treated as Niphotrichum by ), the latter including R. varium (also treated as a Codriophorus in ). .. Total genomic DNA was extracted using the DNeasy® Plant Kit (Qiagen) or the NucleoSpin® Plant II Kit (Macherey-Nagel).

Agarose Gel Electrophoresis:

Article Title: Begoniayenyeniae ( Begoniaceae), a new species from Endau Rompin National Park, Johor, Malaysia
Article Snippet: DNA of Begonia species was extracted from living material or silica gel dried material using the NucleoSpin Plant II kit following the manufacturer’s protocols (Macherey Nagel, Germany). .. The following PCR profile was used: initial denaturation at 95 °C for 5 min, followed by 40 cycles of denaturation at 95 °C for 1 min, primer annealing at 55 °C for 1 min and extension at 72 °C for 2 min; followed by a final extension step at 72 °C for 5 min. Amplicons were visualised using 1.0% agarose gel electrophoresis stained with SYBR Safe DNA Gel Stain (Invitrogen, U.S.A.).

Spectrophotometry:

Article Title: Spatio-Temporal Dynamic of Tuber magnatum Mycelium in Natural Truffle Grounds
Article Snippet: Crude DNA solutions were then purified using the Nucleospin Plant II kit (Macherey-Nagel). .. Total DNAs were quantified by a NanoDrop ND-1000 Spectrophotometer (Thermo Scientific) and their quality evaluated with optical density (OD) 260/280 nm and 260/230 nm ratios.

Sampling:

Article Title: Molecular Species Delimitation in the Racomitrium canescens Complex (Grimmiaceae) and Implications for DNA Barcoding of Species Complexes in Mosses
Article Snippet: Paragraph title: Taxon sampling, DNA extraction, PCR and sequencing ... Total genomic DNA was extracted using the DNeasy® Plant Kit (Qiagen) or the NucleoSpin® Plant II Kit (Macherey-Nagel).

Concentration Assay:

Article Title: Simple sequence repeat markers for kānuka (Kunzea spp.; Myrtaceae) present in New Zealand 1
Article Snippet: DNA was extracted from 20 mg of dried leaf material using the NucleoSpin Plant II kit (PL1 lysis buffer; Macherey-Nagel, Düren, Germany) following manufacturer’s instructions, resulting in 200–800 ng of DNA per sample. .. Concentration of PCR products was adjusted, and 1 μL added to 10 μL Hi-Di formamide (Applied Biosystems, Carlsbad, California, USA) and 0.2 μL GeneScan 600 LIZ Size Standard (Applied Biosystems).

Article Title: Identification of QTLs conferring resistance to downy mildew in legacy cultivars of lettuce
Article Snippet: Lyophilized samples were ground to fine powder using TissueLyser mill, before extracting genomic DNA with NucleoSpin Plant II kit (Macherey-Nagel, Betlehem, PA, USA). .. Concentration and quality of the DNA was analyzed with an ND-1000 Spectrometer (NanoDrop Technologies, Wilmington, DE, USA) and gel electrophoresis.

Lysis:

Article Title: Microbial diversity in biodeteriorated Greek historical documents dating back to the 19th and 20th century: A case study, et al. Microbial diversity in biodeteriorated Greek historical documents dating back to the 19th and 20th century: A case study
Article Snippet: 2.2 DNA extraction and PCR amplification from microbial isolates DNA was extracted from 17 morphologically distinct fungal isolates using the NucleoSpin Plant II Kit (Macherey‐Nagel, GERMANY) according to manufacturer's instructions. .. Briefly, a single CFU was suspended in 20 μl of lysis buffer containing 0.25% (v/v) sodium dodecyl sulfate (Serva, GERMANY) and 0.05 N NaOH (Serva, GERMANY).

Article Title: Simple sequence repeat markers for kānuka (Kunzea spp.; Myrtaceae) present in New Zealand 1
Article Snippet: .. DNA was extracted from 20 mg of dried leaf material using the NucleoSpin Plant II kit (PL1 lysis buffer; Macherey-Nagel, Düren, Germany) following manufacturer’s instructions, resulting in 200–800 ng of DNA per sample. .. PCRs were performed in 15-μL reactions, containing 5–50 ng of DNA, and final concentrations of 0.08 μM forward primer, 0.32 μM reverse primer, 0.32 μM 6-FAM–labeled M13F primer, 1× KAPA plant PCR buffer with dNTPs, 0.3 units KAPA3G Plant DNA Polymerase (Kapa Biosystems, Wilmington, Massachusetts, USA), and PCR-grade H2 O. Thermocycling was conducted on the Bioer GenePro thermocyler (Bioer Technology, Hangzhou, Zhejiang Province, China) using the following conditions: initial denaturation at 95°C for 5 min; 30 cycles of 95°C for 20 s, 55°C for 15 s, and 72°C for 30 s; followed by 10 cycles of 95°C for 20 s, 51°C for 15 s, and 72°C for 30 s; and final extension at 72°C for 10 min. Five-microliter PCR products were separated on 2.5% agarose gels.

Article Title: Identification and Monitoring of Amomi Fructus and its Adulterants Based on DNA Barcoding Analysis and Designed DNA Markers
Article Snippet: .. Preparation of Genomic DNA The genomic DNA was extracted from the Amomi Fructus according to the manual of NucleoSpin® Plant II kit (Macherey-Nagel, Dueren, Germany) with PL1 lysis buffer. ..

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    MACHEREY NAGEL nucleospin plant ii dna kit
    a <t>DNA</t> isolate evaluation - silica dioxide powder, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder. Samples 7b–10b were amplificated twice; b DNA isolate evaluation <t>-NucleoSpin®</t> Plant II/Macherey Nagel Kit, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; c DNA isolate evaluation -DNeasy Plant Mini Kit/Qiagen, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; d DNA isolate evaluation-CTAB method, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder.
    Nucleospin Plant Ii Dna Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nucleospin plant ii dna kit/product/MACHEREY NAGEL
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nucleospin plant ii dna kit - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    82
    MACHEREY NAGEL nucleospin plant ii kit
    Correlation between the total number of sequences and the total number of OTUs (a) and the Shannon index (b). Each color represents the three replicates of each of the samples. The codes indicate the technical procedures used in this paper. For type of trap: PJ, petri dish coated with a mix of petroleum jelly and Vaseline; W1, Whatman filter no. 1; W1g, Whatman filter no. 1 sprayed with a sticky layer; W3, Whatman filter no. 3; W3g, Whatman filter no. 3 sprayed with a sticky layer. For spore recovery protocol: G, grinding; R, rubbing; S, shaking. For DNA extraction kit: mnM, <t>NucleoSpin</t> plant II kit; mnQ, DNeasy plant minikit; mxM, NucleoSpin plant II maxikit; mxQ, DNeasy plant maxikit.
    Nucleospin Plant Ii Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nucleospin plant ii kit/product/MACHEREY NAGEL
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nucleospin plant ii kit - by Bioz Stars, 2020-02
    82/100 stars
      Buy from Supplier

    Image Search Results


    a DNA isolate evaluation - silica dioxide powder, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder. Samples 7b–10b were amplificated twice; b DNA isolate evaluation -NucleoSpin® Plant II/Macherey Nagel Kit, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; c DNA isolate evaluation -DNeasy Plant Mini Kit/Qiagen, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; d DNA isolate evaluation-CTAB method, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder.

    Journal: Environmental Monitoring and Assessment

    Article Title: Isolation of nucleic acids using silicon dioxide powder as a tool for environmental monitoring

    doi: 10.1007/s10661-019-7840-2

    Figure Lengend Snippet: a DNA isolate evaluation - silica dioxide powder, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder. Samples 7b–10b were amplificated twice; b DNA isolate evaluation -NucleoSpin® Plant II/Macherey Nagel Kit, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; c DNA isolate evaluation -DNeasy Plant Mini Kit/Qiagen, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder; d DNA isolate evaluation-CTAB method, PCR amplification of the ITS region. For sample details, see Table 2 . Lane M, 100 bp DNA ladder.

    Article Snippet: CTAB-based lysis buffer (NucleoSpin® Plant II DNA Kit, Macherey Nagel, Düren, Germany)

    Techniques: Polymerase Chain Reaction, Amplification

    Correlation between the total number of sequences and the total number of OTUs (a) and the Shannon index (b). Each color represents the three replicates of each of the samples. The codes indicate the technical procedures used in this paper. For type of trap: PJ, petri dish coated with a mix of petroleum jelly and Vaseline; W1, Whatman filter no. 1; W1g, Whatman filter no. 1 sprayed with a sticky layer; W3, Whatman filter no. 3; W3g, Whatman filter no. 3 sprayed with a sticky layer. For spore recovery protocol: G, grinding; R, rubbing; S, shaking. For DNA extraction kit: mnM, NucleoSpin plant II kit; mnQ, DNeasy plant minikit; mxM, NucleoSpin plant II maxikit; mxQ, DNeasy plant maxikit.

    Journal: Applied and Environmental Microbiology

    Article Title: Assessment of Passive Traps Combined with High-Throughput Sequencing To Study Airborne Fungal Communities

    doi: 10.1128/AEM.02637-17

    Figure Lengend Snippet: Correlation between the total number of sequences and the total number of OTUs (a) and the Shannon index (b). Each color represents the three replicates of each of the samples. The codes indicate the technical procedures used in this paper. For type of trap: PJ, petri dish coated with a mix of petroleum jelly and Vaseline; W1, Whatman filter no. 1; W1g, Whatman filter no. 1 sprayed with a sticky layer; W3, Whatman filter no. 3; W3g, Whatman filter no. 3 sprayed with a sticky layer. For spore recovery protocol: G, grinding; R, rubbing; S, shaking. For DNA extraction kit: mnM, NucleoSpin plant II kit; mnQ, DNeasy plant minikit; mxM, NucleoSpin plant II maxikit; mxQ, DNeasy plant maxikit.

    Article Snippet: Four different plant DNA extraction kits were used and compared during this study: the NucleoSpin plant II kit, the NucleoSpin plant II maxikit (both from Macherey-Nagel, Düren, Germany), the DNeasy plant minikit, and the DNeasy plant maxikit (both from Qiagen, Hilden, Germany).

    Techniques: DNA Extraction

    NMDS representation of all the exposed trap protocols. A protocol was the combination of the type of trap, the spore recovery procedure, the DNA extraction kit, and the ITS barcode. All samples were replicated three times. The NMDS showed acceptable values for stress (0.16) and linear fit ( R 2 = 0.89). The codes indicate the technical procedures used in this paper. For type of trap: PJ, petri dish coated with a mix of petroleum jelly and Vaseline; W1, Whatman filter no. 1; W1g, Whatman filter no. 1 sprayed with a sticky layer; W3, Whatman filter no. 3; W3g, Whatman filter no. 3 sprayed with a sticky layer. For spore recovery protocol: G, grinding; R, rubbing; S, shaking. For DNA extraction kit: mnM, NucleoSpin plant II kit; mnQ, DNeasy plant minikit; mxM, NucleoSpin plant II maxikit; mxQ, DNeasy plant maxikit.

    Journal: Applied and Environmental Microbiology

    Article Title: Assessment of Passive Traps Combined with High-Throughput Sequencing To Study Airborne Fungal Communities

    doi: 10.1128/AEM.02637-17

    Figure Lengend Snippet: NMDS representation of all the exposed trap protocols. A protocol was the combination of the type of trap, the spore recovery procedure, the DNA extraction kit, and the ITS barcode. All samples were replicated three times. The NMDS showed acceptable values for stress (0.16) and linear fit ( R 2 = 0.89). The codes indicate the technical procedures used in this paper. For type of trap: PJ, petri dish coated with a mix of petroleum jelly and Vaseline; W1, Whatman filter no. 1; W1g, Whatman filter no. 1 sprayed with a sticky layer; W3, Whatman filter no. 3; W3g, Whatman filter no. 3 sprayed with a sticky layer. For spore recovery protocol: G, grinding; R, rubbing; S, shaking. For DNA extraction kit: mnM, NucleoSpin plant II kit; mnQ, DNeasy plant minikit; mxM, NucleoSpin plant II maxikit; mxQ, DNeasy plant maxikit.

    Article Snippet: Four different plant DNA extraction kits were used and compared during this study: the NucleoSpin plant II kit, the NucleoSpin plant II maxikit (both from Macherey-Nagel, Düren, Germany), the DNeasy plant minikit, and the DNeasy plant maxikit (both from Qiagen, Hilden, Germany).

    Techniques: DNA Extraction