nucleospin plant ii kit  (MACHEREY NAGEL)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    NucleoSpin Tissue
    Description:

    Catalog Number:
    http://catalog.takara-bio.co.jp/
    Price:
    None
    Score:
    85
    Buy from Supplier


    Structured Review

    MACHEREY NAGEL nucleospin plant ii kit

    https://www.bioz.com/result/nucleospin plant ii kit/product/MACHEREY NAGEL
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    nucleospin plant ii kit - by Bioz Stars, 2019-10
    99/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: The newly-arisen Devil facial tumour disease 2 (DFT2) reveals a mechanism for the emergence of a contagious cancer
    Article Snippet: All products were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel), cloned into a pJET plasmid (CloneJET; ThermoFisher Scientific). .. Plasmid DNA from individual clones was isolated and sequenced using T7 primer.

    Amplification:

    Article Title: Establishment and genomic characterization of the new chordoma cell line Chor-IN-1
    Article Snippet: Primers for PCR amplification and sequencing were designed using the freely available Primer3 software (http://primer3.ut.ee/) and synthesized using an Applied Biosystems 3900 Synthesizer (Thermo Fisher Scientific, Waltham, MA, USA). .. For direct sequencing, the obtained PCR product was electrophoresed in agarose gel, then purified using NucleoSpin Gel and PCR clean-up (Macherey-Nagel, Düren,Germany), according to the manufacturer’s protocol, and subjected to Sanger sequencing with a ABI 3100 Genetic Analyzer instrument (Thermo Fisher Scientific, Waltham, MA, USA) with the same primers used for PCR amplification. .. Chordoma clinical samples and cell lines were profiled using TruSight One (TSO) sequencing panel kit (Illumina, San Diego, CA, USA), following manufacturer’s instructions.

    Article Title: Large-Scale Phylogenomic Analysis Reveals the Complex Evolutionary History of Rabies Virus in Multiple Carnivore Hosts
    Article Snippet: The complete viral genome (excluding the 3’ and 5’ extremities, corresponding to the leader and the trailer regions, respectively) of 160 isolates was amplified with six overlapping PCR fragments by using the Phusion polymerase (ThermoFisher). .. After electrophoresis, each PCR fragment was independently purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and quantified using Picogreen dsDNA quantification kit (Invitrogen).

    Article Title: Screening of microbial communities associated with endive lettuce during postharvest processing on industrial scale
    Article Snippet: The thermal amplification protocol started with an initial step at 95 °C for three min, followed by 25 cycles consisting of a denaturation step at 94 °C for 30 sec, an annealing step at 51 °C for 30 sec and elongation step at 72 °C for 90 sec. After that, a final elongation at 72 °C for eight min was conducted. .. DNA bands of expected size were cut out of the agarose gel and were cleaned up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co. KG, Germany).

    Article Title: Mapping polyclonal antibody responses to bacterial infection using next generation phage display
    Article Snippet: A primer set specific to PC89 vector sequences that flanked the peptide gene and amplified a 330 bp fragment was used in a PCR reaction to amplify the peptide gene sequences, these primers also contained a linker sequence ( , P8 forward and reverse primers). .. PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel).

    Article Title: Karyotype evolution in Phalaris (Poaceae): The role of reductional dysploidy, polyploidy and chromosome alteration in a wide-spread and diverse genus
    Article Snippet: The amplification of specific DNA regions was carried out with the 5S rDNA primers 5SGW (GAG AGA GTA GTA CTA GGA TGG) and p5S1 (CGC TTA ACT TCG GAG TTC TGA TGG) and the primers for regions of the 45S rDNA 18S-F (CCA GTA GTC ATA TGA TTG TCT C) and 18S-R (AGG TTC ACC TAC GGA AAC C). .. PCR products were column-purified with the NucleoSpin Gel and PCR clean-up Kit of Macherey-Nagel and dissolved in H2 O.

    Article Title: Identifying DNA Methylation Biomarkers for Non-Endoscopic Detection of Barrett’s Esophagus
    Article Snippet: Successful amplification was confirmed by agarose gel electrophoresis. .. PCR products were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and quantitated by Qubit.

    Article Title: Microbial inoculants for the biocontrol of Fusarium spp. in durum wheat
    Article Snippet: Two μl of the solutions were then used as template to re-amplify the band fragments using the couple primers 968f-GC/518r, Lac1-f/Lac2-GC andAlfie1-GC/Alfie2-r. Then, the positions of the excised bands in DGGE gel were confirmed with repeated DGGE. .. Bands showing the expected melting position in DGGE gels were amplified with the couple primers, without GC-clamp and the obtained amplicons were purified (Nucleospin gel and PCR clean-up kit; Macherey-Nagel GmbH & Co. KG, Germany). .. Finally, purified PCR products were sequenced by a commercial sequencing facility (EurofinsMWG Operon, Ebersberg, Germany).

    Article Title: Development of a point-of-care-device for fast detection of periodontal pathogens
    Article Snippet: 16S primers and template DNA from E. corrodens were applied to produce a biotinylated amplicon by incorporation of biotinylated dUTPs during PCR. .. The probe was purified with the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany).

    Article Title: Molecular characterization and phylogeny of Linguatulaserrata (Pentastomida: Linguatulidae) based on the nuclear 18S rDNA and mitochondrial cytochrome c oxidase I gene
    Article Snippet: DNA fragments of 18S rDNA and cox1 were amplified by the polymerase chain reaction (PCR) in a total volume of 10 µl , containing 0.25 U TKs Gflex™ DNA polymerase (TaKaRa Bio Inc., Otsu, Japan), 0.2 µl template DNA, 1 µ M each primer and 2× Gflex PCR buffer. .. The PCR products were subsequently purified by gel extraction using a NucleoSpin Gel and PCR clean-up kit (MACHEREY−NAGEL GmbH & Co. KG, Düren, Germany).

    Article Title: First autochthonous cases of canine thelaziosis in Slovakia: a new affected area in Central Europe
    Article Snippet: Both PCR reactions consisted of a polymerase activation step at 94 °C for 2 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72 °C for 30 s, followed by a final extension at 72 °C for 7 min. After amplification, PCR products were visualized using 1.2% agarose gel electrophoresis. .. Consequently, amplicons were purified using NucleoSpin® Gel and a PCR Clean-up kit (Macherey-Nagel GmbH & Co., KG, Düren, Germany) and sequenced in both directions.

    Article Title: Epitope mapping reveals the binding mechanism of a functional antibody cross-reactive to both human and murine programmed death 1
    Article Snippet: In the second PCR step, a pair of primers (pCMV: 5′-GCGTTGACATTGATTATTGAC; pTK: 5′-GGAAGAAAGCGAAAGGAGC) were designed for the amplification of the linear DNA expression cassette, which was composed of a CMV promoter, an extracellular domain (ECD) of PD-1, a His-tag and a herpes simplex virus thymidine kinase (TK) polyadenylation. .. Different PD-1 expression cassettes were generated by using an overlap extension polymerase chain reaction (OE-PCR) technique and were extracted from agarose gels using NucleoSpin™ Gel and PCR Clean-up Kit (Macherey & Nagel, 740609.250).

    Article Title: Bite Injuries of Grey Seals (Halichoerus grypus) on Harbour Porpoises (Phocoena phocoena)
    Article Snippet: Amplification by PCR was performed in 25 µl, with ∼100 ng DNA, 0,5 µM of each primer and 12,5 µl PCR Mastermix (2x) (Thermo Scientific, USA). .. Subsequently, bands were cut and purified with the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel, Germany).

    Positive Control:

    Article Title: Development of a point-of-care-device for fast detection of periodontal pathogens
    Article Snippet: The samples, along with the 16S PCR amplicon as positive control, were separated on a 1 % agarose gel at 4 °C. .. The probe was purified with the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany).

    Synthesized:

    Article Title: Establishment and genomic characterization of the new chordoma cell line Chor-IN-1
    Article Snippet: Primers for PCR amplification and sequencing were designed using the freely available Primer3 software (http://primer3.ut.ee/) and synthesized using an Applied Biosystems 3900 Synthesizer (Thermo Fisher Scientific, Waltham, MA, USA). .. For direct sequencing, the obtained PCR product was electrophoresed in agarose gel, then purified using NucleoSpin Gel and PCR clean-up (Macherey-Nagel, Düren,Germany), according to the manufacturer’s protocol, and subjected to Sanger sequencing with a ABI 3100 Genetic Analyzer instrument (Thermo Fisher Scientific, Waltham, MA, USA) with the same primers used for PCR amplification.

    Neutralization:

    Article Title: Development of a point-of-care-device for fast detection of periodontal pathogens
    Article Snippet: The gel was prepared for capillary blot with depurination (0.25 M HCl), denaturation (1.5 M NaCl, 0.5 M NaOH) and neutralization (3 M NaCl, 0.5 M Tris-HCl), followed by overnight blotting onto a nylon membrane. .. The probe was purified with the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany).

    Construct:

    Article Title: Large-Scale Phylogenomic Analysis Reveals the Complex Evolutionary History of Rabies Virus in Multiple Carnivore Hosts
    Article Snippet: After electrophoresis, each PCR fragment was independently purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and quantified using Picogreen dsDNA quantification kit (Invitrogen). .. After electrophoresis, each PCR fragment was independently purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and quantified using Picogreen dsDNA quantification kit (Invitrogen).

    Article Title: Screening of microbial communities associated with endive lettuce during postharvest processing on industrial scale
    Article Snippet: 2.5 To receive detailed information about the bacterial community along the endive postharvest processing chain, a bacterial 16S rRNA gene library was constructed for each sampled process step. .. DNA bands of expected size were cut out of the agarose gel and were cleaned up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co. KG, Germany).

    Article Title: Identifying DNA Methylation Biomarkers for Non-Endoscopic Detection of Barrett’s Esophagus
    Article Snippet: Bisulfite-specific, methylation-indifferent PCR primers were constructed as a mixture of primers against converted products of fully methylated or fully unmethylated templates and were used to amplify a differentially-methylated region of the vimentin exon 1 CpG island (previously described, ( )) or CCNA1 ( ). .. PCR products were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and quantitated by Qubit.

    Electrophoresis:

    Article Title: Antibacterial Activities of Bacteria Isolated from the Marine Sponges Isodictya compressa and Higginsia bidentifera Collected from Algoa Bay, South Africa
    Article Snippet: Duplicate amplifications were conducted for each sample, pooled and purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). .. Duplicate amplifications were conducted for each sample, pooled and purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel).

    Article Title: First report of Trichogrammadanausicida and Trichogrammacacaeciae reared from Thaumatotibialeucotreta eggs in Israel
    Article Snippet: Each PCR reaction was examined by electrophoresis and bands were visualised with UV light. .. PCR products were excised from the gel and purified using the Nucleospin Gel and PCR Clean-Up Kit (Macherey-Nagel, Germany).

    Article Title: Large-Scale Phylogenomic Analysis Reveals the Complex Evolutionary History of Rabies Virus in Multiple Carnivore Hosts
    Article Snippet: The complete viral genome (excluding the 3’ and 5’ extremities, corresponding to the leader and the trailer regions, respectively) of 160 isolates was amplified with six overlapping PCR fragments by using the Phusion polymerase (ThermoFisher). .. After electrophoresis, each PCR fragment was independently purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and quantified using Picogreen dsDNA quantification kit (Invitrogen). .. For each sample, all six PCR fragments were pooled with equimolar proportions to obtain 500 ng of dsDNA.

    Article Title: Rotavirus Genomic RNA Complex Forms via Specific RNA–RNA Interactions: Disruption of RNA Complex Inhibits Virus Infectivity
    Article Snippet: The reaction was immediately analyzed by electrophoresis in a 1% agarose gel supplemented with 0.1 mM MgCl2 in a TBM buffer (45 mM Tris-HCl (pH 8.3), 43 mM boric acid, and 0.1 mM MgCl2 ) at 160 V and 4 °C for 2.5 h, followed by staining with 0.01% (w /v ) ethidium bromide. .. For quantitative reverse transcription (qRT)-PCR analysis of heterodimeric RNAs, the shifted bands were extracted from excised gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s procedure.

    Incubation:

    Article Title: Rotavirus Genomic RNA Complex Forms via Specific RNA–RNA Interactions: Disruption of RNA Complex Inhibits Virus Infectivity
    Article Snippet: The mixture was incubated for 3 h at 37 °C, followed by RNase-free DNase One treatment. .. For quantitative reverse transcription (qRT)-PCR analysis of heterodimeric RNAs, the shifted bands were extracted from excised gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s procedure.

    Article Title: Development of a point-of-care-device for fast detection of periodontal pathogens
    Article Snippet: The probe was purified with the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany). .. To ensure a complete target sequence for hybridization, an examination of possible cutting sites within the sequence was conducted: an enzymatic incubation of the 16S amplicon with the aforementioned enzymes and subsequent check by comparing the band size of digested amplicon to the control DNA in an agarose gel was carried out.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Identifying DNA Methylation Biomarkers for Non-Endoscopic Detection of Barrett’s Esophagus
    Article Snippet: PCR products were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and quantitated by Qubit. .. The NEXTflex Rapid DNA-seq kit (BIOO Scientific) was used to prepare indexed libraries for NGS sequencing (Illumina-compatible), and NGS was performed using a MiSeq platform at the McGill University and Génome Québec Innovation Centre, Montréal, Canada.

    Article Title: The newly-arisen Devil facial tumour disease 2 (DFT2) reveals a mechanism for the emergence of a contagious cancer
    Article Snippet: The NucleoSpin totalRNA FFPE kit (Macherey-Nagel) was used to extract RNA from formalin fixed tumour tissues according to the manufacturer’s instructions. .. All products were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel), cloned into a pJET plasmid (CloneJET; ThermoFisher Scientific).

    Electrophoretic Mobility Shift Assay:

    Article Title: Rotavirus Genomic RNA Complex Forms via Specific RNA–RNA Interactions: Disruption of RNA Complex Inhibits Virus Infectivity
    Article Snippet: Paragraph title: 2.3. Electrophoretic Mobility Shift Assay (EMSA) for RNA–RNA Interaction ... For quantitative reverse transcription (qRT)-PCR analysis of heterodimeric RNAs, the shifted bands were extracted from excised gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s procedure.

    Expressing:

    Article Title: Epitope mapping reveals the binding mechanism of a functional antibody cross-reactive to both human and murine programmed death 1
    Article Snippet: Cassettes were purified using AxyPrep™ PCR clean-up kit (Axygen Biosciences, AP-PCR-250). .. Different PD-1 expression cassettes were generated by using an overlap extension polymerase chain reaction (OE-PCR) technique and were extracted from agarose gels using NucleoSpin™ Gel and PCR Clean-up Kit (Macherey & Nagel, 740609.250). .. The FreeStyle™ 293 expression system (Life Technologies, K900001) was used for the expression of PD-1 mutants.

    Hybridization:

    Article Title: Rotavirus Genomic RNA Complex Forms via Specific RNA–RNA Interactions: Disruption of RNA Complex Inhibits Virus Infectivity
    Article Snippet: For quantitative reverse transcription (qRT)-PCR analysis of heterodimeric RNAs, the shifted bands were extracted from excised gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s procedure. .. For quantitative reverse transcription (qRT)-PCR analysis of heterodimeric RNAs, the shifted bands were extracted from excised gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s procedure.

    Article Title: Development of a point-of-care-device for fast detection of periodontal pathogens
    Article Snippet: For hybridization, the Biotin PCR Labeling Master (Jena Bioscience, Jena, Germany) was used to generate a probe. .. The probe was purified with the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany).

    Gas Chromatography:

    Article Title: Microbial inoculants for the biocontrol of Fusarium spp. in durum wheat
    Article Snippet: Two μl of the solutions were then used as template to re-amplify the band fragments using the couple primers 968f-GC/518r, Lac1-f/Lac2-GC andAlfie1-GC/Alfie2-r. Then, the positions of the excised bands in DGGE gel were confirmed with repeated DGGE. .. Bands showing the expected melting position in DGGE gels were amplified with the couple primers, without GC-clamp and the obtained amplicons were purified (Nucleospin gel and PCR clean-up kit; Macherey-Nagel GmbH & Co. KG, Germany). .. Finally, purified PCR products were sequenced by a commercial sequencing facility (EurofinsMWG Operon, Ebersberg, Germany).

    Chromatography:

    Article Title: Rotavirus Genomic RNA Complex Forms via Specific RNA–RNA Interactions: Disruption of RNA Complex Inhibits Virus Infectivity
    Article Snippet: For quantitative reverse transcription (qRT)-PCR analysis of heterodimeric RNAs, the shifted bands were extracted from excised gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s procedure. .. The RNA–ORN hybridization assay was carried out by the 3′ end labeling of 10 pmol of S10 and S11 ORNs (10.2, 10.3 and 11.2 ORNs) including scrambled (Scr) with 10 μCi [32 P]pCp (Perkin Elmer, Waltham, MA, USA) with T4 RNA ligase (Thermo Scientific, Waltham, MA, USA) in a T4 RNA ligase buffer, and incubating at 4 °C overnight.

    Activation Assay:

    Article Title: Identifying DNA Methylation Biomarkers for Non-Endoscopic Detection of Barrett’s Esophagus
    Article Snippet: PCR was performed using a touchdown protocol where after the activation of Taq polymerase at 95°C for 5 min, the initial cycling conditions were: 95°C for 45 sec, 67°C for 45 sec, 72°C for 45 sec. .. PCR products were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and quantitated by Qubit.

    Article Title: First autochthonous cases of canine thelaziosis in Slovakia: a new affected area in Central Europe
    Article Snippet: Both PCR reactions consisted of a polymerase activation step at 94 °C for 2 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72 °C for 30 s, followed by a final extension at 72 °C for 7 min. After amplification, PCR products were visualized using 1.2% agarose gel electrophoresis. .. Consequently, amplicons were purified using NucleoSpin® Gel and a PCR Clean-up kit (Macherey-Nagel GmbH & Co., KG, Düren, Germany) and sequenced in both directions.

    Plasmid Preparation:

    Article Title: Rotavirus Genomic RNA Complex Forms via Specific RNA–RNA Interactions: Disruption of RNA Complex Inhibits Virus Infectivity
    Article Snippet: RNA segments were co-transcribed using 150 ng of linearized plasmid (S8–S11) in a buffer containing 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2 , 20 mM NaCl2 , 3 mM spermidine, 50 mM dithiothreitol (DTT), 5 mM each of rATP, rUTP, rCTP, and rGTP, 10 U of RNase inhibitor and 40 U of T7 RNA polymerase (Thermo Scientific, Waltham, MA, USA). .. For quantitative reverse transcription (qRT)-PCR analysis of heterodimeric RNAs, the shifted bands were extracted from excised gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s procedure.

    Article Title: Screening of microbial communities associated with endive lettuce during postharvest processing on industrial scale
    Article Snippet: DNA bands of expected size were cut out of the agarose gel and were cleaned up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co. KG, Germany). .. DNA bands of expected size were cut out of the agarose gel and were cleaned up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co. KG, Germany).

    Article Title: Mapping polyclonal antibody responses to bacterial infection using next generation phage display
    Article Snippet: A primer set specific to PC89 vector sequences that flanked the peptide gene and amplified a 330 bp fragment was used in a PCR reaction to amplify the peptide gene sequences, these primers also contained a linker sequence ( , P8 forward and reverse primers). .. PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel).

    Article Title: The newly-arisen Devil facial tumour disease 2 (DFT2) reveals a mechanism for the emergence of a contagious cancer
    Article Snippet: Primer set one was used to amplify Saha-UA , -UB and –UC products as a group, primer sets 2 and 3 were used to amplify Saha-UK and Saha-UD genes specifically (for PCR conditions see ). .. All products were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel), cloned into a pJET plasmid (CloneJET; ThermoFisher Scientific). .. Plasmid DNA from individual clones was isolated and sequenced using T7 primer.

    Article Title: Epitope mapping reveals the binding mechanism of a functional antibody cross-reactive to both human and murine programmed death 1
    Article Snippet: A pcDNA3.3-hPD-1_ECD.His plasmid that encodes ECD of human PD-1 and a C-terminal His-tag was used as template, and a set of mutagenic primers with a melting temperature ( Tm ) more than 75°C and 10–15 bases of template-complementary sequence on both sides of desired point mutation codon were used for the first PCR step using the QuikChange lightning multi-site-directed mutagenesis kit (Agilent technologies, 210513), according to the manufacturer's instructions. .. Different PD-1 expression cassettes were generated by using an overlap extension polymerase chain reaction (OE-PCR) technique and were extracted from agarose gels using NucleoSpin™ Gel and PCR Clean-up Kit (Macherey & Nagel, 740609.250).

    Generated:

    Article Title: First report of Trichogrammadanausicida and Trichogrammacacaeciae reared from Thaumatotibialeucotreta eggs in Israel
    Article Snippet: PCR products were excised from the gel and purified using the Nucleospin Gel and PCR Clean-Up Kit (Macherey-Nagel, Germany). .. PCR products were excised from the gel and purified using the Nucleospin Gel and PCR Clean-Up Kit (Macherey-Nagel, Germany).

    Article Title: Epitope mapping reveals the binding mechanism of a functional antibody cross-reactive to both human and murine programmed death 1
    Article Snippet: Cassettes were purified using AxyPrep™ PCR clean-up kit (Axygen Biosciences, AP-PCR-250). .. Different PD-1 expression cassettes were generated by using an overlap extension polymerase chain reaction (OE-PCR) technique and were extracted from agarose gels using NucleoSpin™ Gel and PCR Clean-up Kit (Macherey & Nagel, 740609.250). .. The FreeStyle™ 293 expression system (Life Technologies, K900001) was used for the expression of PD-1 mutants.

    Polymerase Chain Reaction:

    Article Title: Antibacterial Activities of Bacteria Isolated from the Marine Sponges Isodictya compressa and Higginsia bidentifera Collected from Algoa Bay, South Africa
    Article Snippet: The following cycling conditions were used: initial denaturation of 98 °C for 2 min followed by 35 cycles of 98 °C for 20 s, 54 °C for 30 s, 72 °C for 1 min and final elongation at 72 °C for 5 min. .. Duplicate amplifications were conducted for each sample, pooled and purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). .. Amplicons (1 µg) were digested in 30 µL volumes containing 3 µL of 10 × Tango buffer, 2 µL of Hha I (10 U/µL), 4 µL Hae III (10 U/µL), and UV treated water.

    Article Title: Establishment and genomic characterization of the new chordoma cell line Chor-IN-1
    Article Snippet: Primers for PCR amplification and sequencing were designed using the freely available Primer3 software (http://primer3.ut.ee/) and synthesized using an Applied Biosystems 3900 Synthesizer (Thermo Fisher Scientific, Waltham, MA, USA). .. For direct sequencing, the obtained PCR product was electrophoresed in agarose gel, then purified using NucleoSpin Gel and PCR clean-up (Macherey-Nagel, Düren,Germany), according to the manufacturer’s protocol, and subjected to Sanger sequencing with a ABI 3100 Genetic Analyzer instrument (Thermo Fisher Scientific, Waltham, MA, USA) with the same primers used for PCR amplification. .. Chordoma clinical samples and cell lines were profiled using TruSight One (TSO) sequencing panel kit (Illumina, San Diego, CA, USA), following manufacturer’s instructions.

    Article Title: First report of Trichogrammadanausicida and Trichogrammacacaeciae reared from Thaumatotibialeucotreta eggs in Israel
    Article Snippet: Each PCR reaction was examined by electrophoresis and bands were visualised with UV light. .. PCR products were excised from the gel and purified using the Nucleospin Gel and PCR Clean-Up Kit (Macherey-Nagel, Germany). .. Purified PCR products were sequenced in both the forward and reverse directions (HyLabs, Rehovot, Israel).

    Article Title: Longitudinal study of age-specific pattern of coronavirus infection in Lyle’s flying fox (Pteropus lylei) in Thailand
    Article Snippet: All positive PCR products were further sequenced for confirmation and strain characterization. .. The RdRp PCR products were gel purified using the NucleoSpin® Gel and PCR Clean-up kit (MACHEREY-NAGEL GmbH & Co. KG), and sequenced directly using an automated ABI PRISM 377 DNA sequencer. .. When multi peaks were shown in chromatogram at same position from direct sequencing, PCR products were cloned using the pGEM®-T Easy Vector System and the LigaFast™ Rapid DNA Ligation System (Promega) before sequencing.

    Article Title: Large-Scale Phylogenomic Analysis Reveals the Complex Evolutionary History of Rabies Virus in Multiple Carnivore Hosts
    Article Snippet: The complete viral genome (excluding the 3’ and 5’ extremities, corresponding to the leader and the trailer regions, respectively) of 160 isolates was amplified with six overlapping PCR fragments by using the Phusion polymerase (ThermoFisher). .. After electrophoresis, each PCR fragment was independently purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and quantified using Picogreen dsDNA quantification kit (Invitrogen). .. For each sample, all six PCR fragments were pooled with equimolar proportions to obtain 500 ng of dsDNA.

    Article Title: Rotavirus Genomic RNA Complex Forms via Specific RNA–RNA Interactions: Disruption of RNA Complex Inhibits Virus Infectivity
    Article Snippet: The integrity of the transcribed RNA was checked by denaturing gel electrophoresis on a 1% agarose gel with formaldehyde (10 mL of 10× MOPS running buffer) and 18 mL of 37% formaldehyde (12 M) on a pH 7.0 1× MOPS running buffer (0.4 M MOPS, 1 M sodium acetate, and 0.01 M ethylenediaminetetraacetic acid (EDTA)). .. For quantitative reverse transcription (qRT)-PCR analysis of heterodimeric RNAs, the shifted bands were extracted from excised gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s procedure. .. The RNA–ORN hybridization assay was carried out by the 3′ end labeling of 10 pmol of S10 and S11 ORNs (10.2, 10.3 and 11.2 ORNs) including scrambled (Scr) with 10 μCi [32 P]pCp (Perkin Elmer, Waltham, MA, USA) with T4 RNA ligase (Thermo Scientific, Waltham, MA, USA) in a T4 RNA ligase buffer, and incubating at 4 °C overnight.

    Article Title: Screening of microbial communities associated with endive lettuce during postharvest processing on industrial scale
    Article Snippet: The reagent mixture consisted of 1x Taq buffer, 2 mM MgCl2 , 0.2 mM dNTPs, 0.4 μM each primer, 1 U recombinant Taq polymerase and 1 μl template DNA in a total volume of 50 μl (all reagents purchased by Thermo Fisher Scientific Inc, Germany). .. DNA bands of expected size were cut out of the agarose gel and were cleaned up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co. KG, Germany). .. Ligation of PCR fragments into the pGEM®-T vector system and the transformation of vectors into JM109 competent cells was done according to the manufacturer's protocol (Promega Corporation, USA).

    Article Title: Mapping polyclonal antibody responses to bacterial infection using next generation phage display
    Article Snippet: A primer set specific to PC89 vector sequences that flanked the peptide gene and amplified a 330 bp fragment was used in a PCR reaction to amplify the peptide gene sequences, these primers also contained a linker sequence ( , P8 forward and reverse primers). .. PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). .. Purified product (1 μl) was amplified with a second primer set ( , Akey-BC-linker1(Forward) and P1prim-linker2(Reverse) primers) that included a distinct barcode ( ) sequence and A and P1 adapter sequences designed for the Ion Torrent sequencing platform.

    Article Title: Karyotype evolution in Phalaris (Poaceae): The role of reductional dysploidy, polyploidy and chromosome alteration in a wide-spread and diverse genus
    Article Snippet: Amplifications were performed on an Eppendorf Mastercycler (Hamburg, Germany) using a reaction mix containing 0.5 μ M each of forward and reverse primer, 2 μ l of 10×Taq specific buffer (Quiagen, Benelux B.V., The Netherlands), 1.75 mM MgCl2 , 1 U Taq DNA polymerase (Qiagen), 200 μ M dNTPs (GeneCraft, Lüdinghausen, Germany), 1 ng/μl of template DNA and an aliquot of purified water to obtain a final volume of 20 μ l. The PCR program was for 5S: 55min [94°C 3 min, (94°C 30 s, 55°C 30 s, 72°C 5 min) × 35, 72°C 1 min, 8°C stop] and for 18S: [94°C 3 min, (94°C 30 s, 51°C 30 s, 72°C 2 min) × 35, 72°C 1 min, 8°C stop]. .. PCR products were column-purified with the NucleoSpin Gel and PCR clean-up Kit of Macherey-Nagel and dissolved in H2 O. .. The probes were labelled with fluorescein-dUTP (45S rDNA)- and tetramethylrhodamine-dUTP (5S rDNA) using the Nick-Translation Mix of Roche Diagnostics (Mannheim, Germany) according to the manufacturer’s instructions.

    Article Title: Wisconsin microbiome study, a cross-sectional investigation of dietary fibre, microbiome composition and antibiotic-resistant organisms: rationale and methods
    Article Snippet: Finally, dried DNA pellets are resuspended in 100 µL TE buffer and stored overnight at 4°C or at 37°C for 1 hour to dissolve the DNA pellet. .. Samples are then purified using NucleoSpin Gel and PCR clean-up kit according to manufacturer’s directions (Macherey-Nagel, Germany) and eluted in 40 µL TE buffer. .. DNA is quantified using PicoGreen in a microplate reader (BioTek Instruments) and stored long term at −80°C.

    Article Title: Identifying DNA Methylation Biomarkers for Non-Endoscopic Detection of Barrett’s Esophagus
    Article Snippet: Successful amplification was confirmed by agarose gel electrophoresis. .. PCR products were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and quantitated by Qubit. .. The NEXTflex Rapid DNA-seq kit (BIOO Scientific) was used to prepare indexed libraries for NGS sequencing (Illumina-compatible), and NGS was performed using a MiSeq platform at the McGill University and Génome Québec Innovation Centre, Montréal, Canada.

    Article Title: Screening of Ready-to-Eat Meat Products for Hepatitis E Virus in Switzerland
    Article Snippet: Cycling conditions were as follows: 95 °C for 3 min, then 40 cycles each with 94 °C for 30 s, 50 °C for 30 s and 72 °C for 60 s, with a final extension at 72 °C for 5 min. PCR products were separated on 1.5% agarose gel and visualized with HD Green Plus DNA stain (Intas) under UV-light. .. The inner nested PCR products were purified (NucleoSpin® Gel and PCR Clean-up Kit, Macherey–Nagel) and DNA strands were sequenced (Microsynth) in separate forward- and reverse-primer reactions. .. The edited nucleotide sequences were analysed for phylogenetic relationship using the web service Phylogeny.fr (Dereeper et al. , ).

    Article Title: Microbial inoculants for the biocontrol of Fusarium spp. in durum wheat
    Article Snippet: Two μl of the solutions were then used as template to re-amplify the band fragments using the couple primers 968f-GC/518r, Lac1-f/Lac2-GC andAlfie1-GC/Alfie2-r. Then, the positions of the excised bands in DGGE gel were confirmed with repeated DGGE. .. Bands showing the expected melting position in DGGE gels were amplified with the couple primers, without GC-clamp and the obtained amplicons were purified (Nucleospin gel and PCR clean-up kit; Macherey-Nagel GmbH & Co. KG, Germany). .. Finally, purified PCR products were sequenced by a commercial sequencing facility (EurofinsMWG Operon, Ebersberg, Germany).

    Article Title: Development of a point-of-care-device for fast detection of periodontal pathogens
    Article Snippet: 16S primers and template DNA from E. corrodens were applied to produce a biotinylated amplicon by incorporation of biotinylated dUTPs during PCR. .. The probe was purified with the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany). .. To ensure a complete target sequence for hybridization, an examination of possible cutting sites within the sequence was conducted: an enzymatic incubation of the 16S amplicon with the aforementioned enzymes and subsequent check by comparing the band size of digested amplicon to the control DNA in an agarose gel was carried out.

    Article Title: Molecular characterization and phylogeny of Linguatulaserrata (Pentastomida: Linguatulidae) based on the nuclear 18S rDNA and mitochondrial cytochrome c oxidase I gene
    Article Snippet: PCR products were separated by 1.2% agarose gel electrophoresis, stained with ethidium bromide and visualized with an ultraviolet transilluminator. .. The PCR products were subsequently purified by gel extraction using a NucleoSpin Gel and PCR clean-up kit (MACHEREY−NAGEL GmbH & Co. KG, Düren, Germany). .. The PCR amplicons for both 18S rDNA and cox1 were sequenced directly from both directions using a BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, U.S.A.).

    Article Title: First autochthonous cases of canine thelaziosis in Slovakia: a new affected area in Central Europe
    Article Snippet: Both PCR reactions consisted of a polymerase activation step at 94 °C for 2 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72 °C for 30 s, followed by a final extension at 72 °C for 7 min. After amplification, PCR products were visualized using 1.2% agarose gel electrophoresis. .. Consequently, amplicons were purified using NucleoSpin® Gel and a PCR Clean-up kit (Macherey-Nagel GmbH & Co., KG, Düren, Germany) and sequenced in both directions. .. Nucleotide sequences were manually edited and further compared with GenBank entries using BLAST (Basic Local Alignment Search Tool).

    Article Title: The newly-arisen Devil facial tumour disease 2 (DFT2) reveals a mechanism for the emergence of a contagious cancer
    Article Snippet: Primer set one was used to amplify Saha-UA , -UB and –UC products as a group, primer sets 2 and 3 were used to amplify Saha-UK and Saha-UD genes specifically (for PCR conditions see ). .. All products were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel), cloned into a pJET plasmid (CloneJET; ThermoFisher Scientific). .. Plasmid DNA from individual clones was isolated and sequenced using T7 primer.

    Article Title: Epitope mapping reveals the binding mechanism of a functional antibody cross-reactive to both human and murine programmed death 1
    Article Snippet: Cassettes were purified using AxyPrep™ PCR clean-up kit (Axygen Biosciences, AP-PCR-250). .. Different PD-1 expression cassettes were generated by using an overlap extension polymerase chain reaction (OE-PCR) technique and were extracted from agarose gels using NucleoSpin™ Gel and PCR Clean-up Kit (Macherey & Nagel, 740609.250). .. The FreeStyle™ 293 expression system (Life Technologies, K900001) was used for the expression of PD-1 mutants.

    Article Title: Bite Injuries of Grey Seals (Halichoerus grypus) on Harbour Porpoises (Phocoena phocoena)
    Article Snippet: PCR products were subjected to electrophoresis on 1% agarose gel (SYBR Safe DNA Gel Stain, Invitrogen, USA) and visualized via UV light. .. Subsequently, bands were cut and purified with the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel, Germany). .. Purified products were cloned in pCRII-Topo plasmid (Invitrogen, USA) and sequenced with automated BigDye capillary sequencer ABI 3730 (Applied Biosystems, Life Technologies, USA), following the manufacturer's recommendations.

    Overlap Extension Polymerase Chain Reaction:

    Article Title: Epitope mapping reveals the binding mechanism of a functional antibody cross-reactive to both human and murine programmed death 1
    Article Snippet: Cassettes were purified using AxyPrep™ PCR clean-up kit (Axygen Biosciences, AP-PCR-250). .. Different PD-1 expression cassettes were generated by using an overlap extension polymerase chain reaction (OE-PCR) technique and were extracted from agarose gels using NucleoSpin™ Gel and PCR Clean-up Kit (Macherey & Nagel, 740609.250). .. The FreeStyle™ 293 expression system (Life Technologies, K900001) was used for the expression of PD-1 mutants.

    DNA Sequencing:

    Article Title: Large-Scale Phylogenomic Analysis Reveals the Complex Evolutionary History of Rabies Virus in Multiple Carnivore Hosts
    Article Snippet: After electrophoresis, each PCR fragment was independently purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and quantified using Picogreen dsDNA quantification kit (Invitrogen). .. After electrophoresis, each PCR fragment was independently purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and quantified using Picogreen dsDNA quantification kit (Invitrogen).

    Sequencing:

    Article Title: Establishment and genomic characterization of the new chordoma cell line Chor-IN-1
    Article Snippet: Primers for PCR amplification and sequencing were designed using the freely available Primer3 software (http://primer3.ut.ee/) and synthesized using an Applied Biosystems 3900 Synthesizer (Thermo Fisher Scientific, Waltham, MA, USA). .. For direct sequencing, the obtained PCR product was electrophoresed in agarose gel, then purified using NucleoSpin Gel and PCR clean-up (Macherey-Nagel, Düren,Germany), according to the manufacturer’s protocol, and subjected to Sanger sequencing with a ABI 3100 Genetic Analyzer instrument (Thermo Fisher Scientific, Waltham, MA, USA) with the same primers used for PCR amplification. .. Chordoma clinical samples and cell lines were profiled using TruSight One (TSO) sequencing panel kit (Illumina, San Diego, CA, USA), following manufacturer’s instructions.

    Article Title: First report of Trichogrammadanausicida and Trichogrammacacaeciae reared from Thaumatotibialeucotreta eggs in Israel
    Article Snippet: PCR products were excised from the gel and purified using the Nucleospin Gel and PCR Clean-Up Kit (Macherey-Nagel, Germany). .. PCR products were excised from the gel and purified using the Nucleospin Gel and PCR Clean-Up Kit (Macherey-Nagel, Germany).

    Article Title: Longitudinal study of age-specific pattern of coronavirus infection in Lyle’s flying fox (Pteropus lylei) in Thailand
    Article Snippet: Paragraph title: Sequencing and phylogenetic analysis ... The RdRp PCR products were gel purified using the NucleoSpin® Gel and PCR Clean-up kit (MACHEREY-NAGEL GmbH & Co. KG), and sequenced directly using an automated ABI PRISM 377 DNA sequencer.

    Article Title: Screening of microbial communities associated with endive lettuce during postharvest processing on industrial scale
    Article Snippet: Paragraph title: 16S rRNA gene nucleotide sequence analysis ... DNA bands of expected size were cut out of the agarose gel and were cleaned up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co. KG, Germany).

    Article Title: Mapping polyclonal antibody responses to bacterial infection using next generation phage display
    Article Snippet: Paragraph title: DNA extraction and sample preparation for Ion Torrent sequencing ... PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel).

    Article Title: Microbial inoculants for the biocontrol of Fusarium spp. in durum wheat
    Article Snippet: Bands showing the expected melting position in DGGE gels were amplified with the couple primers, without GC-clamp and the obtained amplicons were purified (Nucleospin gel and PCR clean-up kit; Macherey-Nagel GmbH & Co. KG, Germany). .. Bands showing the expected melting position in DGGE gels were amplified with the couple primers, without GC-clamp and the obtained amplicons were purified (Nucleospin gel and PCR clean-up kit; Macherey-Nagel GmbH & Co. KG, Germany).

    Article Title: Molecular characterization and phylogeny of Linguatulaserrata (Pentastomida: Linguatulidae) based on the nuclear 18S rDNA and mitochondrial cytochrome c oxidase I gene
    Article Snippet: The PCR products were subsequently purified by gel extraction using a NucleoSpin Gel and PCR clean-up kit (MACHEREY−NAGEL GmbH & Co. KG, Düren, Germany). .. The PCR amplicons for both 18S rDNA and cox1 were sequenced directly from both directions using a BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, U.S.A.).

    Article Title: The newly-arisen Devil facial tumour disease 2 (DFT2) reveals a mechanism for the emergence of a contagious cancer
    Article Snippet: Paragraph title: RT-PCR and sequencing of MHC class I transcripts in DFT2, DFT1 and host devils ... All products were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel), cloned into a pJET plasmid (CloneJET; ThermoFisher Scientific).

    Article Title: Epitope mapping reveals the binding mechanism of a functional antibody cross-reactive to both human and murine programmed death 1
    Article Snippet: A pcDNA3.3-hPD-1_ECD.His plasmid that encodes ECD of human PD-1 and a C-terminal His-tag was used as template, and a set of mutagenic primers with a melting temperature ( Tm ) more than 75°C and 10–15 bases of template-complementary sequence on both sides of desired point mutation codon were used for the first PCR step using the QuikChange lightning multi-site-directed mutagenesis kit (Agilent technologies, 210513), according to the manufacturer's instructions. .. Different PD-1 expression cassettes were generated by using an overlap extension polymerase chain reaction (OE-PCR) technique and were extracted from agarose gels using NucleoSpin™ Gel and PCR Clean-up Kit (Macherey & Nagel, 740609.250).

    Recombinant:

    Article Title: Screening of microbial communities associated with endive lettuce during postharvest processing on industrial scale
    Article Snippet: The reagent mixture consisted of 1x Taq buffer, 2 mM MgCl2 , 0.2 mM dNTPs, 0.4 μM each primer, 1 U recombinant Taq polymerase and 1 μl template DNA in a total volume of 50 μl (all reagents purchased by Thermo Fisher Scientific Inc, Germany). .. DNA bands of expected size were cut out of the agarose gel and were cleaned up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co. KG, Germany).

    Cellular Antioxidant Activity Assay:

    Article Title: First autochthonous cases of canine thelaziosis in Slovakia: a new affected area in Central Europe
    Article Snippet: PCR targeting of a fragment of 28S rRNA gene was performed using a set of universal nematode primers C1 (5'-ACC CGC TGA ATT TAA GCA T-3') and D2 (5'-TCC GTG TTT CAA GAC GG-3') [ ]. .. Consequently, amplicons were purified using NucleoSpin® Gel and a PCR Clean-up kit (Macherey-Nagel GmbH & Co., KG, Düren, Germany) and sequenced in both directions.

    DNA Extraction:

    Article Title: Antibacterial Activities of Bacteria Isolated from the Marine Sponges Isodictya compressa and Higginsia bidentifera Collected from Algoa Bay, South Africa
    Article Snippet: The UltraClean Soil DNA Isolation kit (MOBIO Laboratories, Carlsbad, CA, USA) was used to isolate DNA according to the manufactures protocol. .. Duplicate amplifications were conducted for each sample, pooled and purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel).

    Article Title: Mapping polyclonal antibody responses to bacterial infection using next generation phage display
    Article Snippet: Paragraph title: DNA extraction and sample preparation for Ion Torrent sequencing ... PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel).

    Article Title: Wisconsin microbiome study, a cross-sectional investigation of dietary fibre, microbiome composition and antibiotic-resistant organisms: rationale and methods
    Article Snippet: Paragraph title: Stool genomic DNA extraction ... Samples are then purified using NucleoSpin Gel and PCR clean-up kit according to manufacturer’s directions (Macherey-Nagel, Germany) and eluted in 40 µL TE buffer.

    Nucleic Acid Electrophoresis:

    Article Title: Rotavirus Genomic RNA Complex Forms via Specific RNA–RNA Interactions: Disruption of RNA Complex Inhibits Virus Infectivity
    Article Snippet: The integrity of the transcribed RNA was checked by denaturing gel electrophoresis on a 1% agarose gel with formaldehyde (10 mL of 10× MOPS running buffer) and 18 mL of 37% formaldehyde (12 M) on a pH 7.0 1× MOPS running buffer (0.4 M MOPS, 1 M sodium acetate, and 0.01 M ethylenediaminetetraacetic acid (EDTA)). .. For quantitative reverse transcription (qRT)-PCR analysis of heterodimeric RNAs, the shifted bands were extracted from excised gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s procedure.

    Methylation:

    Article Title: Identifying DNA Methylation Biomarkers for Non-Endoscopic Detection of Barrett’s Esophagus
    Article Snippet: Paragraph title: Bisulfite-Sequencing-based methylation detection (Bisulfite-Seq ... PCR products were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and quantitated by Qubit.

    Mutagenesis:

    Article Title: Epitope mapping reveals the binding mechanism of a functional antibody cross-reactive to both human and murine programmed death 1
    Article Snippet: Dpn I endonuclease was used to digest the parental template after mutant strand synthesis reaction. .. Different PD-1 expression cassettes were generated by using an overlap extension polymerase chain reaction (OE-PCR) technique and were extracted from agarose gels using NucleoSpin™ Gel and PCR Clean-up Kit (Macherey & Nagel, 740609.250).

    Isolation:

    Article Title: Screening of microbial communities associated with endive lettuce during postharvest processing on industrial scale
    Article Snippet: DNA bands of expected size were cut out of the agarose gel and were cleaned up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co. KG, Germany). .. DNA bands of expected size were cut out of the agarose gel and were cleaned up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co. KG, Germany).

    Article Title: Karyotype evolution in Phalaris (Poaceae): The role of reductional dysploidy, polyploidy and chromosome alteration in a wide-spread and diverse genus
    Article Snippet: For FISH, total genomic DNA was isolated from silica gel-dried fresh leaves of Melica ciliata (HB15/1) using the NucleoSpin Plant II Kit of Marcherey-Nagel (Düren, Germany) according to the manufacturer’s instructions. .. PCR products were column-purified with the NucleoSpin Gel and PCR clean-up Kit of Macherey-Nagel and dissolved in H2 O.

    Article Title: First autochthonous cases of canine thelaziosis in Slovakia: a new affected area in Central Europe
    Article Snippet: DNA was extracted using a commercial isolation set DNeasy Blood & Tissue Kit (Qiagen®, Hilden, Germany) according to the manufacturer’s instructions. .. Consequently, amplicons were purified using NucleoSpin® Gel and a PCR Clean-up kit (Macherey-Nagel GmbH & Co., KG, Düren, Germany) and sequenced in both directions.

    Article Title: The newly-arisen Devil facial tumour disease 2 (DFT2) reveals a mechanism for the emergence of a contagious cancer
    Article Snippet: All products were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel), cloned into a pJET plasmid (CloneJET; ThermoFisher Scientific). .. For primer set 1 between 15 and 65 clones were sequenced for each PCR, for primer sets 2 and 3 between 4 and 6 clones were sequenced.

    Size-exclusion Chromatography:

    Article Title: Establishment and genomic characterization of the new chordoma cell line Chor-IN-1
    Article Snippet: The DNA polymorphism (c.G530A, p.G177D) in the “T” gene (Brachyury) was characterized by PCR amplification using the following primer pairs: FW 5′-TCACCAACAAGCTCAACGGA-3′; REV 5′-AAGCAGTCACCGCTATGAAC-3′ (1 cycle: 95 °C for 1 min; 30 cycles: 95 °C for 30 sec, 53 °C for 30 sec, 72 °C for 1 min; 1 cycle: 68 °C for 5 min) in a total reaction volume of 50 µl, containing 1× PCR buffer, 0.2 mM each dNTP mix, 0.8 µM of each primer, 1.25 units of FideliTaq DNA Polymerase (Affymetrix, Santa Clara, CA, USA) and 100 ng of genomic DNA as template. .. For direct sequencing, the obtained PCR product was electrophoresed in agarose gel, then purified using NucleoSpin Gel and PCR clean-up (Macherey-Nagel, Düren,Germany), according to the manufacturer’s protocol, and subjected to Sanger sequencing with a ABI 3100 Genetic Analyzer instrument (Thermo Fisher Scientific, Waltham, MA, USA) with the same primers used for PCR amplification.

    Article Title: First report of Trichogrammadanausicida and Trichogrammacacaeciae reared from Thaumatotibialeucotreta eggs in Israel
    Article Snippet: PCR products were excised from the gel and purified using the Nucleospin Gel and PCR Clean-Up Kit (Macherey-Nagel, Germany). .. PCR products were excised from the gel and purified using the Nucleospin Gel and PCR Clean-Up Kit (Macherey-Nagel, Germany).

    Article Title: Screening of microbial communities associated with endive lettuce during postharvest processing on industrial scale
    Article Snippet: The thermal amplification protocol started with an initial step at 95 °C for three min, followed by 25 cycles consisting of a denaturation step at 94 °C for 30 sec, an annealing step at 51 °C for 30 sec and elongation step at 72 °C for 90 sec. After that, a final elongation at 72 °C for eight min was conducted. .. DNA bands of expected size were cut out of the agarose gel and were cleaned up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co. KG, Germany).

    Article Title: Identifying DNA Methylation Biomarkers for Non-Endoscopic Detection of Barrett’s Esophagus
    Article Snippet: PCR was performed using a touchdown protocol where after the activation of Taq polymerase at 95°C for 5 min, the initial cycling conditions were: 95°C for 45 sec, 67°C for 45 sec, 72°C for 45 sec. .. PCR products were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and quantitated by Qubit.

    Article Title: Molecular characterization and phylogeny of Linguatulaserrata (Pentastomida: Linguatulidae) based on the nuclear 18S rDNA and mitochondrial cytochrome c oxidase I gene
    Article Snippet: The reaction cycle for 18S rDNA consisted of an initial denaturation at 94°C for 1 min; followed by 40 cycles at 98°C for 10 sec, 60°C for 15 sec and 68°C for 30 sec, whereas that for cox1 consisted of an initial denaturation at 95°C for 15 min; followed by 45 cycles at 94°C for 45 sec, 47°C for 45 sec and 72°C for 90 sec; with a final extension at 72°C for 10 min. .. The PCR products were subsequently purified by gel extraction using a NucleoSpin Gel and PCR clean-up kit (MACHEREY−NAGEL GmbH & Co. KG, Düren, Germany).

    Article Title: Bite Injuries of Grey Seals (Halichoerus grypus) on Harbour Porpoises (Phocoena phocoena)
    Article Snippet: PCR reactions were performed on a Mastercycler Pro Thermocycler (Eppendorf, Germany) with the following parameters: 95°C for 2 min, then 40 cycles of 95°C for 20 sec, 43°C for 15 sec and 72°C for 45 sec, followed by 72°C for 2 min. .. Subsequently, bands were cut and purified with the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel, Germany).

    Labeling:

    Article Title: Development of a point-of-care-device for fast detection of periodontal pathogens
    Article Snippet: For hybridization, the Biotin PCR Labeling Master (Jena Bioscience, Jena, Germany) was used to generate a probe. .. The probe was purified with the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany).

    Purification:

    Article Title: Antibacterial Activities of Bacteria Isolated from the Marine Sponges Isodictya compressa and Higginsia bidentifera Collected from Algoa Bay, South Africa
    Article Snippet: The following cycling conditions were used: initial denaturation of 98 °C for 2 min followed by 35 cycles of 98 °C for 20 s, 54 °C for 30 s, 72 °C for 1 min and final elongation at 72 °C for 5 min. .. Duplicate amplifications were conducted for each sample, pooled and purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). .. Amplicons (1 µg) were digested in 30 µL volumes containing 3 µL of 10 × Tango buffer, 2 µL of Hha I (10 U/µL), 4 µL Hae III (10 U/µL), and UV treated water.

    Article Title: Establishment and genomic characterization of the new chordoma cell line Chor-IN-1
    Article Snippet: Primers for PCR amplification and sequencing were designed using the freely available Primer3 software (http://primer3.ut.ee/) and synthesized using an Applied Biosystems 3900 Synthesizer (Thermo Fisher Scientific, Waltham, MA, USA). .. For direct sequencing, the obtained PCR product was electrophoresed in agarose gel, then purified using NucleoSpin Gel and PCR clean-up (Macherey-Nagel, Düren,Germany), according to the manufacturer’s protocol, and subjected to Sanger sequencing with a ABI 3100 Genetic Analyzer instrument (Thermo Fisher Scientific, Waltham, MA, USA) with the same primers used for PCR amplification. .. Chordoma clinical samples and cell lines were profiled using TruSight One (TSO) sequencing panel kit (Illumina, San Diego, CA, USA), following manufacturer’s instructions.

    Article Title: First report of Trichogrammadanausicida and Trichogrammacacaeciae reared from Thaumatotibialeucotreta eggs in Israel
    Article Snippet: Each PCR reaction was examined by electrophoresis and bands were visualised with UV light. .. PCR products were excised from the gel and purified using the Nucleospin Gel and PCR Clean-Up Kit (Macherey-Nagel, Germany). .. Purified PCR products were sequenced in both the forward and reverse directions (HyLabs, Rehovot, Israel).

    Article Title: Longitudinal study of age-specific pattern of coronavirus infection in Lyle’s flying fox (Pteropus lylei) in Thailand
    Article Snippet: All positive PCR products were further sequenced for confirmation and strain characterization. .. The RdRp PCR products were gel purified using the NucleoSpin® Gel and PCR Clean-up kit (MACHEREY-NAGEL GmbH & Co. KG), and sequenced directly using an automated ABI PRISM 377 DNA sequencer. .. When multi peaks were shown in chromatogram at same position from direct sequencing, PCR products were cloned using the pGEM®-T Easy Vector System and the LigaFast™ Rapid DNA Ligation System (Promega) before sequencing.

    Article Title: Large-Scale Phylogenomic Analysis Reveals the Complex Evolutionary History of Rabies Virus in Multiple Carnivore Hosts
    Article Snippet: The complete viral genome (excluding the 3’ and 5’ extremities, corresponding to the leader and the trailer regions, respectively) of 160 isolates was amplified with six overlapping PCR fragments by using the Phusion polymerase (ThermoFisher). .. After electrophoresis, each PCR fragment was independently purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and quantified using Picogreen dsDNA quantification kit (Invitrogen). .. For each sample, all six PCR fragments were pooled with equimolar proportions to obtain 500 ng of dsDNA.

    Article Title: Mapping polyclonal antibody responses to bacterial infection using next generation phage display
    Article Snippet: A primer set specific to PC89 vector sequences that flanked the peptide gene and amplified a 330 bp fragment was used in a PCR reaction to amplify the peptide gene sequences, these primers also contained a linker sequence ( , P8 forward and reverse primers). .. PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). .. Purified product (1 μl) was amplified with a second primer set ( , Akey-BC-linker1(Forward) and P1prim-linker2(Reverse) primers) that included a distinct barcode ( ) sequence and A and P1 adapter sequences designed for the Ion Torrent sequencing platform.

    Article Title: Karyotype evolution in Phalaris (Poaceae): The role of reductional dysploidy, polyploidy and chromosome alteration in a wide-spread and diverse genus
    Article Snippet: Amplifications were performed on an Eppendorf Mastercycler (Hamburg, Germany) using a reaction mix containing 0.5 μ M each of forward and reverse primer, 2 μ l of 10×Taq specific buffer (Quiagen, Benelux B.V., The Netherlands), 1.75 mM MgCl2 , 1 U Taq DNA polymerase (Qiagen), 200 μ M dNTPs (GeneCraft, Lüdinghausen, Germany), 1 ng/μl of template DNA and an aliquot of purified water to obtain a final volume of 20 μ l. The PCR program was for 5S: 55min [94°C 3 min, (94°C 30 s, 55°C 30 s, 72°C 5 min) × 35, 72°C 1 min, 8°C stop] and for 18S: [94°C 3 min, (94°C 30 s, 51°C 30 s, 72°C 2 min) × 35, 72°C 1 min, 8°C stop]. .. PCR products were column-purified with the NucleoSpin Gel and PCR clean-up Kit of Macherey-Nagel and dissolved in H2 O.

    Article Title: Wisconsin microbiome study, a cross-sectional investigation of dietary fibre, microbiome composition and antibiotic-resistant organisms: rationale and methods
    Article Snippet: Finally, dried DNA pellets are resuspended in 100 µL TE buffer and stored overnight at 4°C or at 37°C for 1 hour to dissolve the DNA pellet. .. Samples are then purified using NucleoSpin Gel and PCR clean-up kit according to manufacturer’s directions (Macherey-Nagel, Germany) and eluted in 40 µL TE buffer. .. DNA is quantified using PicoGreen in a microplate reader (BioTek Instruments) and stored long term at −80°C.

    Article Title: Identifying DNA Methylation Biomarkers for Non-Endoscopic Detection of Barrett’s Esophagus
    Article Snippet: Successful amplification was confirmed by agarose gel electrophoresis. .. PCR products were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and quantitated by Qubit. .. The NEXTflex Rapid DNA-seq kit (BIOO Scientific) was used to prepare indexed libraries for NGS sequencing (Illumina-compatible), and NGS was performed using a MiSeq platform at the McGill University and Génome Québec Innovation Centre, Montréal, Canada.

    Article Title: Screening of Ready-to-Eat Meat Products for Hepatitis E Virus in Switzerland
    Article Snippet: Cycling conditions were as follows: 95 °C for 3 min, then 40 cycles each with 94 °C for 30 s, 50 °C for 30 s and 72 °C for 60 s, with a final extension at 72 °C for 5 min. PCR products were separated on 1.5% agarose gel and visualized with HD Green Plus DNA stain (Intas) under UV-light. .. The inner nested PCR products were purified (NucleoSpin® Gel and PCR Clean-up Kit, Macherey–Nagel) and DNA strands were sequenced (Microsynth) in separate forward- and reverse-primer reactions. .. The edited nucleotide sequences were analysed for phylogenetic relationship using the web service Phylogeny.fr (Dereeper et al. , ).

    Article Title: Microbial inoculants for the biocontrol of Fusarium spp. in durum wheat
    Article Snippet: Two μl of the solutions were then used as template to re-amplify the band fragments using the couple primers 968f-GC/518r, Lac1-f/Lac2-GC andAlfie1-GC/Alfie2-r. Then, the positions of the excised bands in DGGE gel were confirmed with repeated DGGE. .. Bands showing the expected melting position in DGGE gels were amplified with the couple primers, without GC-clamp and the obtained amplicons were purified (Nucleospin gel and PCR clean-up kit; Macherey-Nagel GmbH & Co. KG, Germany). .. Finally, purified PCR products were sequenced by a commercial sequencing facility (EurofinsMWG Operon, Ebersberg, Germany).

    Article Title: Development of a point-of-care-device for fast detection of periodontal pathogens
    Article Snippet: 16S primers and template DNA from E. corrodens were applied to produce a biotinylated amplicon by incorporation of biotinylated dUTPs during PCR. .. The probe was purified with the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany). .. To ensure a complete target sequence for hybridization, an examination of possible cutting sites within the sequence was conducted: an enzymatic incubation of the 16S amplicon with the aforementioned enzymes and subsequent check by comparing the band size of digested amplicon to the control DNA in an agarose gel was carried out.

    Article Title: Molecular characterization and phylogeny of Linguatulaserrata (Pentastomida: Linguatulidae) based on the nuclear 18S rDNA and mitochondrial cytochrome c oxidase I gene
    Article Snippet: PCR products were separated by 1.2% agarose gel electrophoresis, stained with ethidium bromide and visualized with an ultraviolet transilluminator. .. The PCR products were subsequently purified by gel extraction using a NucleoSpin Gel and PCR clean-up kit (MACHEREY−NAGEL GmbH & Co. KG, Düren, Germany). .. The PCR amplicons for both 18S rDNA and cox1 were sequenced directly from both directions using a BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, U.S.A.).

    Article Title: First autochthonous cases of canine thelaziosis in Slovakia: a new affected area in Central Europe
    Article Snippet: Both PCR reactions consisted of a polymerase activation step at 94 °C for 2 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72 °C for 30 s, followed by a final extension at 72 °C for 7 min. After amplification, PCR products were visualized using 1.2% agarose gel electrophoresis. .. Consequently, amplicons were purified using NucleoSpin® Gel and a PCR Clean-up kit (Macherey-Nagel GmbH & Co., KG, Düren, Germany) and sequenced in both directions. .. Nucleotide sequences were manually edited and further compared with GenBank entries using BLAST (Basic Local Alignment Search Tool).

    Article Title: The newly-arisen Devil facial tumour disease 2 (DFT2) reveals a mechanism for the emergence of a contagious cancer
    Article Snippet: Primer set one was used to amplify Saha-UA , -UB and –UC products as a group, primer sets 2 and 3 were used to amplify Saha-UK and Saha-UD genes specifically (for PCR conditions see ). .. All products were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel), cloned into a pJET plasmid (CloneJET; ThermoFisher Scientific). .. Plasmid DNA from individual clones was isolated and sequenced using T7 primer.

    Article Title: Epitope mapping reveals the binding mechanism of a functional antibody cross-reactive to both human and murine programmed death 1
    Article Snippet: Cassettes were purified using AxyPrep™ PCR clean-up kit (Axygen Biosciences, AP-PCR-250). .. Different PD-1 expression cassettes were generated by using an overlap extension polymerase chain reaction (OE-PCR) technique and were extracted from agarose gels using NucleoSpin™ Gel and PCR Clean-up Kit (Macherey & Nagel, 740609.250).

    Article Title: Bite Injuries of Grey Seals (Halichoerus grypus) on Harbour Porpoises (Phocoena phocoena)
    Article Snippet: PCR products were subjected to electrophoresis on 1% agarose gel (SYBR Safe DNA Gel Stain, Invitrogen, USA) and visualized via UV light. .. Subsequently, bands were cut and purified with the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel, Germany). .. Purified products were cloned in pCRII-Topo plasmid (Invitrogen, USA) and sequenced with automated BigDye capillary sequencer ABI 3730 (Applied Biosystems, Life Technologies, USA), following the manufacturer's recommendations.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The newly-arisen Devil facial tumour disease 2 (DFT2) reveals a mechanism for the emergence of a contagious cancer
    Article Snippet: Paragraph title: RT-PCR and sequencing of MHC class I transcripts in DFT2, DFT1 and host devils ... All products were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel), cloned into a pJET plasmid (CloneJET; ThermoFisher Scientific).

    Quantitative RT-PCR:

    Article Title: Rotavirus Genomic RNA Complex Forms via Specific RNA–RNA Interactions: Disruption of RNA Complex Inhibits Virus Infectivity
    Article Snippet: The integrity of the transcribed RNA was checked by denaturing gel electrophoresis on a 1% agarose gel with formaldehyde (10 mL of 10× MOPS running buffer) and 18 mL of 37% formaldehyde (12 M) on a pH 7.0 1× MOPS running buffer (0.4 M MOPS, 1 M sodium acetate, and 0.01 M ethylenediaminetetraacetic acid (EDTA)). .. For quantitative reverse transcription (qRT)-PCR analysis of heterodimeric RNAs, the shifted bands were extracted from excised gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s procedure. .. The RNA–ORN hybridization assay was carried out by the 3′ end labeling of 10 pmol of S10 and S11 ORNs (10.2, 10.3 and 11.2 ORNs) including scrambled (Scr) with 10 μCi [32 P]pCp (Perkin Elmer, Waltham, MA, USA) with T4 RNA ligase (Thermo Scientific, Waltham, MA, USA) in a T4 RNA ligase buffer, and incubating at 4 °C overnight.

    Staining:

    Article Title: Rotavirus Genomic RNA Complex Forms via Specific RNA–RNA Interactions: Disruption of RNA Complex Inhibits Virus Infectivity
    Article Snippet: The reaction was immediately analyzed by electrophoresis in a 1% agarose gel supplemented with 0.1 mM MgCl2 in a TBM buffer (45 mM Tris-HCl (pH 8.3), 43 mM boric acid, and 0.1 mM MgCl2 ) at 160 V and 4 °C for 2.5 h, followed by staining with 0.01% (w /v ) ethidium bromide. .. For quantitative reverse transcription (qRT)-PCR analysis of heterodimeric RNAs, the shifted bands were extracted from excised gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s procedure.

    Article Title: Screening of Ready-to-Eat Meat Products for Hepatitis E Virus in Switzerland
    Article Snippet: Cycling conditions were as follows: 95 °C for 3 min, then 40 cycles each with 94 °C for 30 s, 50 °C for 30 s and 72 °C for 60 s, with a final extension at 72 °C for 5 min. PCR products were separated on 1.5% agarose gel and visualized with HD Green Plus DNA stain (Intas) under UV-light. .. The inner nested PCR products were purified (NucleoSpin® Gel and PCR Clean-up Kit, Macherey–Nagel) and DNA strands were sequenced (Microsynth) in separate forward- and reverse-primer reactions.

    Article Title: Microbial inoculants for the biocontrol of Fusarium spp. in durum wheat
    Article Snippet: Gels were stained in a solution of 1X SYBR-Green (Sigma–Aldrich) in 1X TAE for 20 min and their images captured in UV transillumination with Gel DocTM 226 XR apparatus (Bio-Rad). .. Bands showing the expected melting position in DGGE gels were amplified with the couple primers, without GC-clamp and the obtained amplicons were purified (Nucleospin gel and PCR clean-up kit; Macherey-Nagel GmbH & Co. KG, Germany).

    Article Title: Molecular characterization and phylogeny of Linguatulaserrata (Pentastomida: Linguatulidae) based on the nuclear 18S rDNA and mitochondrial cytochrome c oxidase I gene
    Article Snippet: PCR products were separated by 1.2% agarose gel electrophoresis, stained with ethidium bromide and visualized with an ultraviolet transilluminator. .. The PCR products were subsequently purified by gel extraction using a NucleoSpin Gel and PCR clean-up kit (MACHEREY−NAGEL GmbH & Co. KG, Düren, Germany).

    Nested PCR:

    Article Title: Screening of Ready-to-Eat Meat Products for Hepatitis E Virus in Switzerland
    Article Snippet: Cycling conditions were as follows: 95 °C for 3 min, then 40 cycles each with 94 °C for 30 s, 50 °C for 30 s and 72 °C for 60 s, with a final extension at 72 °C for 5 min. PCR products were separated on 1.5% agarose gel and visualized with HD Green Plus DNA stain (Intas) under UV-light. .. The inner nested PCR products were purified (NucleoSpin® Gel and PCR Clean-up Kit, Macherey–Nagel) and DNA strands were sequenced (Microsynth) in separate forward- and reverse-primer reactions. .. The edited nucleotide sequences were analysed for phylogenetic relationship using the web service Phylogeny.fr (Dereeper et al. , ).

    Agarose Gel Electrophoresis:

    Article Title: Establishment and genomic characterization of the new chordoma cell line Chor-IN-1
    Article Snippet: Primers for PCR amplification and sequencing were designed using the freely available Primer3 software (http://primer3.ut.ee/) and synthesized using an Applied Biosystems 3900 Synthesizer (Thermo Fisher Scientific, Waltham, MA, USA). .. For direct sequencing, the obtained PCR product was electrophoresed in agarose gel, then purified using NucleoSpin Gel and PCR clean-up (Macherey-Nagel, Düren,Germany), according to the manufacturer’s protocol, and subjected to Sanger sequencing with a ABI 3100 Genetic Analyzer instrument (Thermo Fisher Scientific, Waltham, MA, USA) with the same primers used for PCR amplification. .. Chordoma clinical samples and cell lines were profiled using TruSight One (TSO) sequencing panel kit (Illumina, San Diego, CA, USA), following manufacturer’s instructions.

    Article Title: Rotavirus Genomic RNA Complex Forms via Specific RNA–RNA Interactions: Disruption of RNA Complex Inhibits Virus Infectivity
    Article Snippet: The integrity of the transcribed RNA was checked by denaturing gel electrophoresis on a 1% agarose gel with formaldehyde (10 mL of 10× MOPS running buffer) and 18 mL of 37% formaldehyde (12 M) on a pH 7.0 1× MOPS running buffer (0.4 M MOPS, 1 M sodium acetate, and 0.01 M ethylenediaminetetraacetic acid (EDTA)). .. For quantitative reverse transcription (qRT)-PCR analysis of heterodimeric RNAs, the shifted bands were extracted from excised gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s procedure.

    Article Title: Screening of microbial communities associated with endive lettuce during postharvest processing on industrial scale
    Article Snippet: The reagent mixture consisted of 1x Taq buffer, 2 mM MgCl2 , 0.2 mM dNTPs, 0.4 μM each primer, 1 U recombinant Taq polymerase and 1 μl template DNA in a total volume of 50 μl (all reagents purchased by Thermo Fisher Scientific Inc, Germany). .. DNA bands of expected size were cut out of the agarose gel and were cleaned up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co. KG, Germany). .. Ligation of PCR fragments into the pGEM®-T vector system and the transformation of vectors into JM109 competent cells was done according to the manufacturer's protocol (Promega Corporation, USA).

    Article Title: Mapping polyclonal antibody responses to bacterial infection using next generation phage display
    Article Snippet: PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). .. Samples were multiplexed (50 different barcoded primers were available; see ) in each sequencing run.

    Article Title: Identifying DNA Methylation Biomarkers for Non-Endoscopic Detection of Barrett’s Esophagus
    Article Snippet: Successful amplification was confirmed by agarose gel electrophoresis. .. PCR products were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and quantitated by Qubit.

    Article Title: Screening of Ready-to-Eat Meat Products for Hepatitis E Virus in Switzerland
    Article Snippet: Cycling conditions were as follows: 95 °C for 3 min, then 40 cycles each with 94 °C for 30 s, 50 °C for 30 s and 72 °C for 60 s, with a final extension at 72 °C for 5 min. PCR products were separated on 1.5% agarose gel and visualized with HD Green Plus DNA stain (Intas) under UV-light. .. The inner nested PCR products were purified (NucleoSpin® Gel and PCR Clean-up Kit, Macherey–Nagel) and DNA strands were sequenced (Microsynth) in separate forward- and reverse-primer reactions.

    Article Title: Development of a point-of-care-device for fast detection of periodontal pathogens
    Article Snippet: The samples, along with the 16S PCR amplicon as positive control, were separated on a 1 % agarose gel at 4 °C. .. The probe was purified with the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany).

    Article Title: Molecular characterization and phylogeny of Linguatulaserrata (Pentastomida: Linguatulidae) based on the nuclear 18S rDNA and mitochondrial cytochrome c oxidase I gene
    Article Snippet: PCR products were separated by 1.2% agarose gel electrophoresis, stained with ethidium bromide and visualized with an ultraviolet transilluminator. .. The PCR products were subsequently purified by gel extraction using a NucleoSpin Gel and PCR clean-up kit (MACHEREY−NAGEL GmbH & Co. KG, Düren, Germany).

    Article Title: First autochthonous cases of canine thelaziosis in Slovakia: a new affected area in Central Europe
    Article Snippet: Both PCR reactions consisted of a polymerase activation step at 94 °C for 2 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72 °C for 30 s, followed by a final extension at 72 °C for 7 min. After amplification, PCR products were visualized using 1.2% agarose gel electrophoresis. .. Consequently, amplicons were purified using NucleoSpin® Gel and a PCR Clean-up kit (Macherey-Nagel GmbH & Co., KG, Düren, Germany) and sequenced in both directions.

    Article Title: Bite Injuries of Grey Seals (Halichoerus grypus) on Harbour Porpoises (Phocoena phocoena)
    Article Snippet: PCR products were subjected to electrophoresis on 1% agarose gel (SYBR Safe DNA Gel Stain, Invitrogen, USA) and visualized via UV light. .. Subsequently, bands were cut and purified with the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel, Germany).

    Activated Clotting Time Assay:

    Article Title: Screening of microbial communities associated with endive lettuce during postharvest processing on industrial scale
    Article Snippet: Therefore, a Bacteria -specific PCR was performed using the unlabelled forward primer 27f and the reverse primer 1492r (5′- TAC GGY TAC CTT GTT ACG ACT T -3′) ( Biomers.net GmbH, Germany) in order to amplify a nearly full length 16S rRNA gene sequence. .. DNA bands of expected size were cut out of the agarose gel and were cleaned up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co. KG, Germany).

    Article Title: Karyotype evolution in Phalaris (Poaceae): The role of reductional dysploidy, polyploidy and chromosome alteration in a wide-spread and diverse genus
    Article Snippet: The amplification of specific DNA regions was carried out with the 5S rDNA primers 5SGW (GAG AGA GTA GTA CTA GGA TGG) and p5S1 (CGC TTA ACT TCG GAG TTC TGA TGG) and the primers for regions of the 45S rDNA 18S-F (CCA GTA GTC ATA TGA TTG TCT C) and 18S-R (AGG TTC ACC TAC GGA AAC C). .. PCR products were column-purified with the NucleoSpin Gel and PCR clean-up Kit of Macherey-Nagel and dissolved in H2 O.

    Terminal Restriction Fragment Length Polymorphism:

    Article Title: Antibacterial Activities of Bacteria Isolated from the Marine Sponges Isodictya compressa and Higginsia bidentifera Collected from Algoa Bay, South Africa
    Article Snippet: Paragraph title: 2.2. Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis ... Duplicate amplifications were conducted for each sample, pooled and purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel).

    Software:

    Article Title: Antibacterial Activities of Bacteria Isolated from the Marine Sponges Isodictya compressa and Higginsia bidentifera Collected from Algoa Bay, South Africa
    Article Snippet: Duplicate amplifications were conducted for each sample, pooled and purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). .. The marker used for the analysis was GS500LIZ.

    Article Title: Establishment and genomic characterization of the new chordoma cell line Chor-IN-1
    Article Snippet: Primers for PCR amplification and sequencing were designed using the freely available Primer3 software (http://primer3.ut.ee/) and synthesized using an Applied Biosystems 3900 Synthesizer (Thermo Fisher Scientific, Waltham, MA, USA). .. For direct sequencing, the obtained PCR product was electrophoresed in agarose gel, then purified using NucleoSpin Gel and PCR clean-up (Macherey-Nagel, Düren,Germany), according to the manufacturer’s protocol, and subjected to Sanger sequencing with a ABI 3100 Genetic Analyzer instrument (Thermo Fisher Scientific, Waltham, MA, USA) with the same primers used for PCR amplification.

    Article Title: Microbial inoculants for the biocontrol of Fusarium spp. in durum wheat
    Article Snippet: Similarities between the DGGE profiles were determined by calculating similarity indices of the densitometric curves of the profiles using Pearson correlation coefficient with the aid of computer software (Gel Compare II, Applied Maths, Keistraat, Belgium). .. Bands showing the expected melting position in DGGE gels were amplified with the couple primers, without GC-clamp and the obtained amplicons were purified (Nucleospin gel and PCR clean-up kit; Macherey-Nagel GmbH & Co. KG, Germany).

    RNA Extraction:

    Article Title: Large-Scale Phylogenomic Analysis Reveals the Complex Evolutionary History of Rabies Virus in Multiple Carnivore Hosts
    Article Snippet: Paragraph title: RNA extraction and next-generation sequencing ... After electrophoresis, each PCR fragment was independently purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and quantified using Picogreen dsDNA quantification kit (Invitrogen).

    Denaturing Gradient Gel Electrophoresis:

    Article Title: Microbial inoculants for the biocontrol of Fusarium spp. in durum wheat
    Article Snippet: Two μl of the solutions were then used as template to re-amplify the band fragments using the couple primers 968f-GC/518r, Lac1-f/Lac2-GC andAlfie1-GC/Alfie2-r. Then, the positions of the excised bands in DGGE gel were confirmed with repeated DGGE. .. Bands showing the expected melting position in DGGE gels were amplified with the couple primers, without GC-clamp and the obtained amplicons were purified (Nucleospin gel and PCR clean-up kit; Macherey-Nagel GmbH & Co. KG, Germany). .. Finally, purified PCR products were sequenced by a commercial sequencing facility (EurofinsMWG Operon, Ebersberg, Germany).

    Sample Prep:

    Article Title: Mapping polyclonal antibody responses to bacterial infection using next generation phage display
    Article Snippet: Paragraph title: DNA extraction and sample preparation for Ion Torrent sequencing ... PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel).

    Southern Blot:

    Article Title: Development of a point-of-care-device for fast detection of periodontal pathogens
    Article Snippet: Paragraph title: Southern blot ... The probe was purified with the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany).

    Next-Generation Sequencing:

    Article Title: Large-Scale Phylogenomic Analysis Reveals the Complex Evolutionary History of Rabies Virus in Multiple Carnivore Hosts
    Article Snippet: Paragraph title: RNA extraction and next-generation sequencing ... After electrophoresis, each PCR fragment was independently purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and quantified using Picogreen dsDNA quantification kit (Invitrogen).

    Article Title: Identifying DNA Methylation Biomarkers for Non-Endoscopic Detection of Barrett’s Esophagus
    Article Snippet: PCR products were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and quantitated by Qubit. .. Analysis of non-endoscopic balloon DNA samples was done the same way as for brushing samples above, except that the PCR amplification primers ( ) were indexed by adding 96 different 7 bp index tags to the 5’ end of both forward and reverse primers ( ).

    Produced:

    Article Title: Mapping polyclonal antibody responses to bacterial infection using next generation phage display
    Article Snippet: Amplicon containing the peptide gene was produced ( ). .. PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel).

    Concentration Assay:

    Article Title: Identifying DNA Methylation Biomarkers for Non-Endoscopic Detection of Barrett’s Esophagus
    Article Snippet: Platinum Taq reaction mix (Invitrogen) was supplemented with 1 mM MgCl2 , 0.2 mM dNTP mix (New England Biolabs), 0.5 M Betaine (Sigma), and a mix of the 4 primers, each at 0.1 µM final concentration. .. PCR products were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and quantitated by Qubit.

    Bisulfite Sequencing:

    Article Title: Identifying DNA Methylation Biomarkers for Non-Endoscopic Detection of Barrett’s Esophagus
    Article Snippet: Paragraph title: Bisulfite-Sequencing-based methylation detection (Bisulfite-Seq ... PCR products were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and quantitated by Qubit.

    Marker:

    Article Title: Antibacterial Activities of Bacteria Isolated from the Marine Sponges Isodictya compressa and Higginsia bidentifera Collected from Algoa Bay, South Africa
    Article Snippet: Duplicate amplifications were conducted for each sample, pooled and purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). .. Digestions were performed in a Bio-Rad T100 Thermal Cycler (Bio-Rad, Hercules, CA, USA) overnight at 37 °C, followed by inactivation at 80 °C for 20 min. Capillary electrophoresis and analysis of the fluorescently labelled terminal restriction fragments (T-RFs) was carried out on an automated ABI3130XL genetic analyzer (Applied Biosystems, Foster City, CA, USA) by the Central Analytical Facility at the University of Stellenbosch, South Africa.

    Gel Extraction:

    Article Title: Molecular characterization and phylogeny of Linguatulaserrata (Pentastomida: Linguatulidae) based on the nuclear 18S rDNA and mitochondrial cytochrome c oxidase I gene
    Article Snippet: PCR products were separated by 1.2% agarose gel electrophoresis, stained with ethidium bromide and visualized with an ultraviolet transilluminator. .. The PCR products were subsequently purified by gel extraction using a NucleoSpin Gel and PCR clean-up kit (MACHEREY−NAGEL GmbH & Co. KG, Düren, Germany). .. The PCR amplicons for both 18S rDNA and cox1 were sequenced directly from both directions using a BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, U.S.A.).

    Fluorescence In Situ Hybridization:

    Article Title: Karyotype evolution in Phalaris (Poaceae): The role of reductional dysploidy, polyploidy and chromosome alteration in a wide-spread and diverse genus
    Article Snippet: For FISH, total genomic DNA was isolated from silica gel-dried fresh leaves of Melica ciliata (HB15/1) using the NucleoSpin Plant II Kit of Marcherey-Nagel (Düren, Germany) according to the manufacturer’s instructions. .. PCR products were column-purified with the NucleoSpin Gel and PCR clean-up Kit of Macherey-Nagel and dissolved in H2 O.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    MACHEREY NAGEL nucleospin plant ii kit
    Nucleospin Plant Ii Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nucleospin plant ii kit/product/MACHEREY NAGEL
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    nucleospin plant ii kit - by Bioz Stars, 2019-10
    99/100 stars
      Buy from Supplier

    99
    MACHEREY NAGEL dna extraction kit nucleospin plant ii
    Dna Extraction Kit Nucleospin Plant Ii, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna extraction kit nucleospin plant ii/product/MACHEREY NAGEL
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dna extraction kit nucleospin plant ii - by Bioz Stars, 2019-10
    99/100 stars
      Buy from Supplier

    Image Search Results