nucaway spin columns  (Thermo Fisher)


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    Structured Review

    Thermo Fisher nucaway spin columns
    Nucaway Spin Columns, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nucaway spin columns/product/Thermo Fisher
    Average 97 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    nucaway spin columns - by Bioz Stars, 2020-04
    97/100 stars

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    Amplification:

    Article Title: Dual-initiation promoters with intertwined canonical and TCT/TOP transcription start sites diversify transcript processing
    Article Snippet: Fluorescent whole-mount in situ hybridization T3 promoter was linked at the 5′ and the 3′ end of the full-length cDNA for each amplified snoRNAs for the synthesis of antisense and Sense riboprobes, respectively. .. The probes were subsequently purified on NucAway spin columns (Ambion), and then ethanol-precipitated.

    In Vitro:

    Article Title: The p22 RNA silencing suppressor of the crinivirus Tomato chlorosis virus preferentially binds long dsRNAs preventing them from cleavage.
    Article Snippet: The PCR products containing the T7 promoter sequence at only one of the ends were used as a template for in vitro transcription. .. Unincorporated nucleotides were removed using NucAway Spin Columns (Ambion).

    Synthesized:

    Article Title: Copper-Induced Expression of a Transmissible Lipoprotein Intramolecular Transacylase Alters Lipoprotein Acylation and the Toll-Like Receptor 2 Response to Listeria monocytogenes
    Article Snippet: .. Biotin-labeled RNA probes were synthesized from DNA using a Maxiscript T7 transcription kit (Thermo Fisher), including the optional DNase digestion and cleanup with NucAway spin columns (Invitrogen), along with gene-T7-specific primer sets (see Table S1). .. Probes were added to 10 ng/ml in Ultrahyb ultrasensitive hybridization buffer (Invitrogen) and incubated at 72°C for 20 h.

    Isolation:

    Article Title: Circular RNA Vav3 sponges gga-miR-375 to promote epithelial-mesenchymal transition
    Article Snippet: .. Total RNA samples isolated from the liver of three chickens in each group and were treated with DNase I (Ambion Turbo DNA-free kit) and NucAway spin columns (Thermo Fisher Scientific) to remove DNA contamination and salts. .. The RNA was incubated at 70°C for 5 min and then 3 μl of Rnase R was added and incubated at 37°C for 10 min. After the reaction, the treated RNA was purified using the RNeasy MinElute Cleanup kits (Qiagen, Valencia, CA, USA) and Agencourt RNAClean XP (Beckman Coulter, Brea, CA, USA).

    Northern Blot:

    Article Title: Copper-Induced Expression of a Transmissible Lipoprotein Intramolecular Transacylase Alters Lipoprotein Acylation and the Toll-Like Receptor 2 Response to Listeria monocytogenes
    Article Snippet: Paragraph title: Northern blotting. ... Biotin-labeled RNA probes were synthesized from DNA using a Maxiscript T7 transcription kit (Thermo Fisher), including the optional DNase digestion and cleanup with NucAway spin columns (Invitrogen), along with gene-T7-specific primer sets (see Table S1).

    Labeling:

    Article Title: Dual-initiation promoters with intertwined canonical and TCT/TOP transcription start sites diversify transcript processing
    Article Snippet: Transcription were done by T3 polymerase using digoxigenin (DIG) labeling mix (Roche) or DNP-11-UTP (TSA™ Plus system, Perkin Elmer) according to manufacturer’s instructions. .. The probes were subsequently purified on NucAway spin columns (Ambion), and then ethanol-precipitated.

    Article Title: The p22 RNA silencing suppressor of the crinivirus Tomato chlorosis virus preferentially binds long dsRNAs preventing them from cleavage.
    Article Snippet: Paragraph title: Preparation of labeled nucleic acids ... Unincorporated nucleotides were removed using NucAway Spin Columns (Ambion).

    Purification:

    Article Title: Dual-initiation promoters with intertwined canonical and TCT/TOP transcription start sites diversify transcript processing
    Article Snippet: .. The probes were subsequently purified on NucAway spin columns (Ambion), and then ethanol-precipitated. .. Single whole-mount in situ hybridizations were performed as described previously .

    Article Title: Circular RNA Vav3 sponges gga-miR-375 to promote epithelial-mesenchymal transition
    Article Snippet: Total RNA samples isolated from the liver of three chickens in each group and were treated with DNase I (Ambion Turbo DNA-free kit) and NucAway spin columns (Thermo Fisher Scientific) to remove DNA contamination and salts. .. The RNA was incubated at 70°C for 5 min and then 3 μl of Rnase R was added and incubated at 37°C for 10 min. After the reaction, the treated RNA was purified using the RNeasy MinElute Cleanup kits (Qiagen, Valencia, CA, USA) and Agencourt RNAClean XP (Beckman Coulter, Brea, CA, USA).

    Produced:

    Article Title: The p22 RNA silencing suppressor of the crinivirus Tomato chlorosis virus preferentially binds long dsRNAs preventing them from cleavage.
    Article Snippet: Duplex dsRNAs of 562 bp and 100 bp were obtained by annealing the two complementary T7 transcripts produced in vitro, and small RNA (sRNA) duplexes were obtained by annealing the two complementary RNA oligonucleotides. .. Unincorporated nucleotides were removed using NucAway Spin Columns (Ambion).

    Polymerase Chain Reaction:

    Article Title: The p22 RNA silencing suppressor of the crinivirus Tomato chlorosis virus preferentially binds long dsRNAs preventing them from cleavage.
    Article Snippet: The PCR products containing the T7 promoter sequence at only one of the ends were used as a template for in vitro transcription. .. Unincorporated nucleotides were removed using NucAway Spin Columns (Ambion).

    Incubation:

    Article Title: Copper-Induced Expression of a Transmissible Lipoprotein Intramolecular Transacylase Alters Lipoprotein Acylation and the Toll-Like Receptor 2 Response to Listeria monocytogenes
    Article Snippet: Biotin-labeled RNA probes were synthesized from DNA using a Maxiscript T7 transcription kit (Thermo Fisher), including the optional DNase digestion and cleanup with NucAway spin columns (Invitrogen), along with gene-T7-specific primer sets (see Table S1). .. Probes were added to 10 ng/ml in Ultrahyb ultrasensitive hybridization buffer (Invitrogen) and incubated at 72°C for 20 h.

    Article Title: Circular RNA Vav3 sponges gga-miR-375 to promote epithelial-mesenchymal transition
    Article Snippet: Total RNA samples isolated from the liver of three chickens in each group and were treated with DNase I (Ambion Turbo DNA-free kit) and NucAway spin columns (Thermo Fisher Scientific) to remove DNA contamination and salts. .. The RNA was incubated at 70°C for 5 min and then 3 μl of Rnase R was added and incubated at 37°C for 10 min. After the reaction, the treated RNA was purified using the RNeasy MinElute Cleanup kits (Qiagen, Valencia, CA, USA) and Agencourt RNAClean XP (Beckman Coulter, Brea, CA, USA).

    Article Title: The p22 RNA silencing suppressor of the crinivirus Tomato chlorosis virus preferentially binds long dsRNAs preventing them from cleavage.
    Article Snippet: In both cases, to anneal complementary strands, reaction mixtures were incubated for 1 min at 95°C and cooled to room temperature. .. Unincorporated nucleotides were removed using NucAway Spin Columns (Ambion).

    Construct:

    Article Title: Circular RNA Vav3 sponges gga-miR-375 to promote epithelial-mesenchymal transition
    Article Snippet: Total RNA samples isolated from the liver of three chickens in each group and were treated with DNase I (Ambion Turbo DNA-free kit) and NucAway spin columns (Thermo Fisher Scientific) to remove DNA contamination and salts. .. RNA libraries were constructed from the treated RNAs using the TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions.

    Acrylamide Gel Assay:

    Article Title: The p22 RNA silencing suppressor of the crinivirus Tomato chlorosis virus preferentially binds long dsRNAs preventing them from cleavage.
    Article Snippet: Unincorporated nucleotides were removed using NucAway Spin Columns (Ambion). .. For single-and double-strand quantification, RNAs were analyzed in 5% acrylamide gel and stained with ethidium bromide, where the different mobilities of both types of molecules could be clearly observed.

    Staining:

    Article Title: The p22 RNA silencing suppressor of the crinivirus Tomato chlorosis virus preferentially binds long dsRNAs preventing them from cleavage.
    Article Snippet: Unincorporated nucleotides were removed using NucAway Spin Columns (Ambion). .. For single-and double-strand quantification, RNAs were analyzed in 5% acrylamide gel and stained with ethidium bromide, where the different mobilities of both types of molecules could be clearly observed.

    Hybridization:

    Article Title: Copper-Induced Expression of a Transmissible Lipoprotein Intramolecular Transacylase Alters Lipoprotein Acylation and the Toll-Like Receptor 2 Response to Listeria monocytogenes
    Article Snippet: Biotin-labeled RNA probes were synthesized from DNA using a Maxiscript T7 transcription kit (Thermo Fisher), including the optional DNase digestion and cleanup with NucAway spin columns (Invitrogen), along with gene-T7-specific primer sets (see Table S1). .. Probes were added to 10 ng/ml in Ultrahyb ultrasensitive hybridization buffer (Invitrogen) and incubated at 72°C for 20 h.

    Sequencing:

    Article Title: Circular RNA Vav3 sponges gga-miR-375 to promote epithelial-mesenchymal transition
    Article Snippet: Paragraph title: CircRNA sequencing and target site prediction ... Total RNA samples isolated from the liver of three chickens in each group and were treated with DNase I (Ambion Turbo DNA-free kit) and NucAway spin columns (Thermo Fisher Scientific) to remove DNA contamination and salts.

    Article Title: The p22 RNA silencing suppressor of the crinivirus Tomato chlorosis virus preferentially binds long dsRNAs preventing them from cleavage.
    Article Snippet: The PCR products containing the T7 promoter sequence at only one of the ends were used as a template for in vitro transcription. .. Unincorporated nucleotides were removed using NucAway Spin Columns (Ambion).

    In Situ Hybridization:

    Article Title: Dual-initiation promoters with intertwined canonical and TCT/TOP transcription start sites diversify transcript processing
    Article Snippet: Paragraph title: Fluorescent whole-mount in situ hybridization ... The probes were subsequently purified on NucAway spin columns (Ambion), and then ethanol-precipitated.

    In Situ:

    Article Title: Dual-initiation promoters with intertwined canonical and TCT/TOP transcription start sites diversify transcript processing
    Article Snippet: The probes were subsequently purified on NucAway spin columns (Ambion), and then ethanol-precipitated. .. Single whole-mount in situ hybridizations were performed as described previously .

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  • Team
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  • 99
    Thermo Fisher nucaway spin columns
    Nucaway Spin Columns, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nucaway spin columns/product/Thermo Fisher
    Average 99 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    nucaway spin columns - by Bioz Stars, 2020-04
    99/100 stars
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