Journal: ACS chemical biology

Article Title: Conditional covalent lethality driven by oncometabolite accumulation

doi: 10.1021/acschembio.2c00384

Figure Lengend Snippet: (a) Structure of MP-1 clickable probe. (b) Dose-dependent labeling of proteomes by clickable probe. Concentrations: 0, 12.5, 25, 50, 75, 100 μM. (c) Schematic for validating target labeling by MP-1 chemotype. (d) Evaluation of putative MP-1 target enrichment by clickable probe following proteomic incubation (0, 10, 50, 100, 200 and 500 μM, 12 h), click chemistry (biotin-N3), streptavidin enrichment, and immunoblotted for TRMT1, Phosphofructokinase platelet type (PFKP) and Cytosolic iron-sulfur cluster assembly factor NUBP2. Ponceau stain image indicates protein loading for each lane. Western blot procedure described in general methods found in supplementary information. Briefly, following chemiluminescence analysis of TRMT1 the nitrocellulose membrane was stripped and re-probed for PFKP and NUBP2.

Article Snippet: TRMT1 (sc-373687), PFKP (sc-514824) and NUBP2 (sc-376784) antibodies were purchased from Santa Cruz Biotechnologies.

Techniques: Labeling, Incubation, Staining, Western Blot, Membrane

Journal: Frontiers in Oncology

Article Title: Serum autoantibody profiling of oral squamous cell carcinoma patients reveals NUBP2 as a potential diagnostic marker

doi: 10.3389/fonc.2023.1167691

Figure Lengend Snippet: Increased levels (Normalized RFU) of protein autoantibodies in serum samples from patients with oral squamous cell carcinoma (OSCC)as compared to those from healthy volunteers (Control) for (A) GGPS1, (B) KRAS, (C) MAP2K6, (D) NUBP2, (E) NSBP1, (F) PRDX1, (G) PSME3, (H) PTPN20A, (I) PYCR1, (J) RPA2, (K) S100A9, (L) TK1, (M) TSPY2, (N) TSPY3, (O) XAGE4. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.

Article Snippet: The mouse monoclonal anti-NUBP2 antibody was purchased from Santa Cruz Biotechnology Inc. (sc-376784) and used at 1:50 dilution.

Techniques: Control

Journal: Frontiers in Oncology

Article Title: Serum autoantibody profiling of oral squamous cell carcinoma patients reveals NUBP2 as a potential diagnostic marker

doi: 10.3389/fonc.2023.1167691

Figure Lengend Snippet: Details of autoantibodies increased in OSCC serum as compared to controls.

Article Snippet: The mouse monoclonal anti-NUBP2 antibody was purchased from Santa Cruz Biotechnology Inc. (sc-376784) and used at 1:50 dilution.

Techniques: Binding Assay, Expressing, Amplification, Over Expression, Phospho-proteomics

Journal: Frontiers in Oncology

Article Title: Serum autoantibody profiling of oral squamous cell carcinoma patients reveals NUBP2 as a potential diagnostic marker

doi: 10.3389/fonc.2023.1167691

Figure Lengend Snippet: Representative immunohistochemistry images showing positive staining of NUBP2 in Oral squamous cell carcinoma. Parietal cells were used as positive controls.

Article Snippet: The mouse monoclonal anti-NUBP2 antibody was purchased from Santa Cruz Biotechnology Inc. (sc-376784) and used at 1:50 dilution.

Techniques: Immunohistochemistry, Staining

Journal: Frontiers in Oncology

Article Title: Serum autoantibody profiling of oral squamous cell carcinoma patients reveals NUBP2 as a potential diagnostic marker

doi: 10.3389/fonc.2023.1167691

Figure Lengend Snippet: Immunohistochemical results for NUBP2.

Article Snippet: The mouse monoclonal anti-NUBP2 antibody was purchased from Santa Cruz Biotechnology Inc. (sc-376784) and used at 1:50 dilution.

Techniques: Immunohistochemical staining, Staining

Journal: Frontiers in Oncology

Article Title: Serum autoantibody profiling of oral squamous cell carcinoma patients reveals NUBP2 as a potential diagnostic marker

doi: 10.3389/fonc.2023.1167691

Figure Lengend Snippet: (A) NUBP2 expression across sample types in GDC TCGA Head and Neck Cancer RNASeq-HTSeq data from Xena Functional Genomics Explorer. (B) NUBP2 expression across clinical stages in GDC TCGA Head and Neck Cancer RNASeq-HTSeq data from Xena Functional Genomics Explorer. Kaplan Meier plots showing correlation of NUBP2 expression with (C) Overall Survival and (D) Disease Free survival in patients with HNSCC from TCGA. (E) Figure showing sequence similarity of NUBP2 across various species. (F) Conservation of NUBP2 across species.

Article Snippet: The mouse monoclonal anti-NUBP2 antibody was purchased from Santa Cruz Biotechnology Inc. (sc-376784) and used at 1:50 dilution.

Techniques: Expressing, Functional Assay, Sequencing

Journal: PLoS ONE

Article Title: Nubp1 Is Required for Lung Branching Morphogenesis and Distal Progenitor Cell Survival in Mice

doi: 10.1371/journal.pone.0044871

Figure Lengend Snippet: (A–E) Nubp1 and its homolog Nubp2 are expressed in the distal lung epithelium. (A, C) In situ hybridization of Nubp1 and Nubp2 on E11.5 lung whole mounts. (B, D) Q-PCR for Nubp1 and Nubp2 at different developmental stages reveals no expression differences in mutant versus wild-type lungs. (E) PCR amplification of the indicated genes on FACS sorted E18.5 distal lung epithelial cells (GFP +) versus remaining GFP (-) cells. (F) Nubp2 interacts with Nubp1 but not with the mutant protein (Nubp1-m1Nisw). A549 cells overexpressing the indicated genes were processed for immunoprecipitation and analyzed by western blotting. (G–H) Mutation in the Nubp1 gene (Nubp1-m1Nisw) does not alter its cellular localization. A549 cells were stably transfected with GFP-Nubp1 or GFP-Nubp1(m1Nisw) and processed for immunofluorescence.

Article Snippet: For immunoprecipitation, cell lysates were incubated with 1 ug of anti-GFP antibody (Sigma, 1∶1000 dilution) and western blotting was performed with anti-myc or anti-Nubp2 antibody (Sigma, 1∶1000 dilution).

Techniques: In Situ Hybridization, Expressing, Mutagenesis, Amplification, Immunoprecipitation, Western Blot, Stable Transfection, Transfection, Immunofluorescence