non hydrolyzable ligand analog 2 deoxyuridine 5 α β imido triphosphate  (Jena Bioscience)


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    Name:
    dUpNHpp
    Description:

    Catalog Number:
    NU-903L
    Price:
    388.1
    Category:
    Nucleotides Nucleosides
    Size:
    5 x 50 µl
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    Structured Review

    Jena Bioscience non hydrolyzable ligand analog 2 deoxyuridine 5 α β imido triphosphate

    https://www.bioz.com/result/non hydrolyzable ligand analog 2 deoxyuridine 5 α β imido triphosphate/product/Jena Bioscience
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    non hydrolyzable ligand analog 2 deoxyuridine 5 α β imido triphosphate - by Bioz Stars, 2021-05
    86/100 stars

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    Crystallization Assay:

    Article Title: Structural insights into the mechanism defining substrate affinity in Arabidopsis thaliana dUTPase: the role of tryptophan 93 in ligand orientation
    Article Snippet: The assay solution contained 100 mM KCl, 5 mM MgCl2 , and 0.25 mM bicine at pH 7.6. dUTPase, to a final concentration of 50 nM, was rapidly mixed with 1–5 μM dUTP solutions in the stopped-flow system, and absorbance was monitored at 573 nm (Fig. d). .. Crystallization Crystals of holo Arabidopsis dUTPase were grown by vapor diffusion with the hanging drop method using 2 M ammonium sulfate as a precipitant in 50 mM Tris–HCl at pH 7.4 and 5 mM of the non-hydrolyzable ligand analog 2′-deoxyuridine 5′-[(α,β)-imido]-triphosphate (dUpNHpp; Jena Bioscience, Jena, Germany) along with 5 mM MgSO4 (Table ) [ , , ]. .. X-ray diffraction data collection, structural analysis, and structural mining Diffraction data were collected from a single holo dUTPase crystal at the Advanced Photon Source (Argonne, IL, USA) sector 14-BM-C.

    Diffusion-based Assay:

    Article Title: Structural insights into the mechanism defining substrate affinity in Arabidopsis thaliana dUTPase: the role of tryptophan 93 in ligand orientation
    Article Snippet: The assay solution contained 100 mM KCl, 5 mM MgCl2 , and 0.25 mM bicine at pH 7.6. dUTPase, to a final concentration of 50 nM, was rapidly mixed with 1–5 μM dUTP solutions in the stopped-flow system, and absorbance was monitored at 573 nm (Fig. d). .. Crystallization Crystals of holo Arabidopsis dUTPase were grown by vapor diffusion with the hanging drop method using 2 M ammonium sulfate as a precipitant in 50 mM Tris–HCl at pH 7.4 and 5 mM of the non-hydrolyzable ligand analog 2′-deoxyuridine 5′-[(α,β)-imido]-triphosphate (dUpNHpp; Jena Bioscience, Jena, Germany) along with 5 mM MgSO4 (Table ) [ , , ]. .. X-ray diffraction data collection, structural analysis, and structural mining Diffraction data were collected from a single holo dUTPase crystal at the Advanced Photon Source (Argonne, IL, USA) sector 14-BM-C.

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    Jena Bioscience non hydrolyzable ligand analog 2 deoxyuridine 5 α β imido triphosphate
    Non Hydrolyzable Ligand Analog 2 Deoxyuridine 5 α β Imido Triphosphate, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non hydrolyzable ligand analog 2 deoxyuridine 5 α β imido triphosphate/product/Jena Bioscience
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    non hydrolyzable ligand analog 2 deoxyuridine 5 α β imido triphosphate - by Bioz Stars, 2021-05
    86/100 stars
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