dudp (Jena Bioscience)


Name:
dUDP
Description:
Catalog Number:
NU-901L
Price:
369.6
Category:
Nucleotides Nucleosides
Size:
5 x 150 µl
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https://www.bioz.com/result/dudp/product/Jena Bioscience
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1) Product Images from "Nucleotide pyrophosphatase employs a P-loop-like motif to enhance catalytic power and NDP/NTP discrimination"
Article Title: Nucleotide pyrophosphatase employs a P-loop-like motif to enhance catalytic power and NDP/NTP discrimination
Journal: Proceedings of the National Academy of Sciences of the United States of America
doi: 10.1073/pnas.1013872108

Figure Legend Snippet: Effect of mutations in the P-loop-like sequence on nucleotide and PPi binding. Fluorescence intensity titration of the loop Trp is shown upon dUTPase binding to ( A ), dUPNPP (solid symbols) and dUDP (open symbols) (hDUT R / K data shown in the inset separately
Techniques Used: Sequencing, Binding Assay, Fluorescence, Titration
Related Articles
Enzymatic Assay:Article Title: Kinetic properties and inhibition of the dimeric dUTPase-dUDPase from Campylobacter jejuni. Article Snippet: .. The enzyme deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) catalyses the hydrolysis of Concentration Assay:Article Title: Kinetic properties and inhibition of the dimeric dUTPase-dUDPase from Campylobacter jejuni. Article Snippet: .. The enzyme deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) catalyses the hydrolysis of Protein Concentration:Article Title: Nucleotide pyrophosphatase employs a P-loop-like motif to enhance catalytic power and NDP/NTP discrimination Article Snippet: .. Protein concentration was measured using the Bradford method (Bio-Rad Protein Assay) and by UV absorbance ( λ 280 = 10,555 M-1 cm-1 for WT and hDUTarmless , λ 280 = 16,055 M-1 cm-1 for hDUTF 158W , hDUTAR /K , and hDUTST/AA ) and is given in monomers. dUMP, Purification:Article Title: Kinetic mechanism of human dUTPase, an essential nucleotide pyrophosphatase enzyme. Article Snippet: Protein concentration was measured using the Bio-Rad protein assay reagent and by UV absorbance ( 280 10,430 M 1 cm 1 for hDUT and 280 15,930 M 1 cm 1 for hDUTW158) and is given in monomers. .. All measurements were carried out in 20 mM HEPES, pH 7.4, buffer, also containing 40 mM NaCl, 2 mM MgCl2, and 1 mM dithiothreitol (unless otherwise stated), at 20 °C. dUMP, |