dudp (Jena Bioscience)

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Name:

dUDP

Description:

Catalog Number:

NU-901L

Price:

369.6

Category:

Nucleotides Nucleosides

Size:

5 x 150 µl

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Structured Review

Jena Bioscience dudp
Effect of mutations in the P-loop-like sequence on nucleotide and PPi binding. Fluorescence intensity titration of the loop Trp is shown upon dUTPase binding to ( A ), <t>dUPNPP</t> (solid symbols) and <t>dUDP</t> (open symbols) (hDUT R / K data shown in the inset separately

Effect of mutations in the P-loop-like sequence on nucleotide and PPi binding. Fluorescence intensity titration of the loop Trp is shown upon dUTPase binding to ( A ), <t>dUPNPP</t> (solid symbols) and <t>dUDP</t> (open symbols) (hDUT R / K data shown in the inset separately

https://www.bioz.com/result/dudp/product/Jena Bioscience
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99

dudp - by Bioz Stars, 2021-04

88/100 stars

Images

1) Product Images from "Nucleotide pyrophosphatase employs a P-loop-like motif to enhance catalytic power and NDP/NTP discrimination"

Article Title: Nucleotide pyrophosphatase employs a P-loop-like motif to enhance catalytic power and NDP/NTP discrimination

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi: 10.1073/pnas.1013872108

Figure Legend Snippet: Effect of mutations in the P-loop-like sequence on nucleotide and PPi binding. Fluorescence intensity titration of the loop Trp is shown upon dUTPase binding to ( A ), dUPNPP (solid symbols) and dUDP (open symbols) (hDUT R / K data shown in the inset separately

Techniques Used: Sequencing, Binding Assay, Fluorescence, Titration

Enzymatic Assay:

Article Title: Kinetic properties and inhibition of the dimeric dUTPase-dUDPase from Campylobacter jejuni.
Article Snippet: .. The enzyme deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) catalyses the hydrolysis of dUTP to dUMP and PPi thus controlling the incorporation of uracil into DNA genomes. ..

Concentration Assay:

Protein Concentration:

Article Title: Nucleotide pyrophosphatase employs a P-loop-like motif to enhance catalytic power and NDP/NTP discrimination
Article Snippet: .. Protein concentration was measured using the Bradford method (Bio-Rad Protein Assay) and by UV absorbance ( λ 280 = 10,555 M-1 cm-1 for WT and hDUTarmless , λ 280 = 16,055 M-1 cm-1 for hDUTF 158W , hDUTAR /K , and hDUTST/AA ) and is given in monomers. dUMP, dUDP, dUTP, and α,β-imido-dUTP (dUPNPP) where purchased from Jena Bioscience, other chemicals where from Sigma Aldrich. ..

Purification:

Article Title: Kinetic mechanism of human dUTPase, an essential nucleotide pyrophosphatase enzyme.
Article Snippet: Protein concentration was measured using the Bio-Rad protein assay reagent and by UV absorbance ( 280 10,430 M 1 cm 1 for hDUT and 280 15,930 M 1 cm 1 for hDUTW158) and is given in monomers. .. All measurements were carried out in 20 mM HEPES, pH 7.4, buffer, also containing 40 mM NaCl, 2 mM MgCl2, and 1 mM dithiothreitol (unless otherwise stated), at 20 °C. dUMP, dUDP, dUTP, and , -imidodUTP (dUPNPP) were purchased from Jena Bioscience (Germany), and [ -32P]ATP was from Izinta Ltd. Myosin was purified from rabbit skeletal muscle according to Ref. 23. .. Enzyme Activity—Enzyme activity was measured in steadystate pH indicator-based assays as described in Ref. 20 and was typically found to be 6 2 s 1.