apg  (Jena Bioscience)


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  • 92
    Name:
    ApG
    Description:

    Catalog Number:
    NU-888L
    Price:
    596.6
    Category:
    Nucleotides Nucleosides
    Size:
    5 x 10 µl
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    Structured Review

    Jena Bioscience apg

    https://www.bioz.com/result/apg/product/Jena Bioscience
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apg - by Bioz Stars, 2021-06
    92/100 stars

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    Related Articles

    other:

    Article Title: A Mechanism for Priming and Realignment during Influenza A Virus Replication
    Article Snippet: Briefly, reactions were performed with 4-μl mixtures that contained 500 μM ApG (Jena Bioscience), 1 mM DTT, 500 μM UTP (unless indicated otherwise), 500 μM CTP, 500 μM ATP (unless indicated otherwise), 0.7 μM vRNA or cRNA promoter (Sigma), 5 mM MgCl2 , 1 U μl−1 RNasin (Promega), 0.05 μM [α-32 P]GTP (3000 Ci mmol−1 ; Perkin-Elmer), 5% glycerol, 0.05% NP-40, 75 mM NaCl, 10 mM HEPES (pH 7.5), and ∼2 ng RdRp μl−1 .

    In Vitro:

    Article Title: Determination of the vRNA and cRNA promoter activity by M segment-specific non-coding nucleotides of influenza A virus
    Article Snippet: The reaction was stopped by addition of 5 µl of 2× RNA loading dye (Invitrogen) and analysed on an 8 M urea/20% denaturing PAGE gel. .. For in vitro pApG-primed synthesis assay, the dinucleotide primer was prepared by labelling of 1 µM ApG (Jena Bioscience) with 0.5 μM [γ-32P]ATP (6,000 Ci/mmole, Perkin-Elmer) and 1 U/µl T4 polynucleotide kinase (T4 PNK) (Enzynomics) in 10 μl 1× T4 PNK reaction buffer (Enzynomics) at 37°C for 1 h[ ]. .. The RdRp assay was performed as mentioned above but with some key modifications in which ATP and [α-32P]GTP were replaced with radiolabeled pApG and 1 mM CTP (for cRNA synthesis) or UTP (for vRNA synthesis) without SAP treatment.

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  • 92
    Jena Bioscience apg
    Apg, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apg/product/Jena Bioscience
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apg - by Bioz Stars, 2021-06
    92/100 stars
      Buy from Supplier

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