biotin 16 utp  (Jena Bioscience)


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  • 94
    Name:
    Biotin 16 UTP
    Description:

    Catalog Number:
    NU-821-BIO16
    Price:
    171.06
    Category:
    Nucleotides Nucleosides
    Size:
    30 µl
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    Structured Review

    Jena Bioscience biotin 16 utp

    https://www.bioz.com/result/biotin 16 utp/product/Jena Bioscience
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin 16 utp - by Bioz Stars, 2021-06
    94/100 stars

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    Related Articles

    In Vitro:

    Article Title: Interaction of 7SK with the Smn complex modulates snRNP production
    Article Snippet: For preparation of biotinylated U2 snRNA, the mouse U2 snRNA gene was PCR-amplified with primers T7-U2-F and U2-R (Supplementary Table ). .. In vitro transcription was performed with T7 RNA Polymerase (Thermo Fisher Scientific) with a 1:10 ratio of biotin-16-UTP/UTP (Jena Bioscience, NU-821-BIO16) and 0.25 U/50 μl inorganic pyrophosphatase (Thermo Fisher Scientific) overnight at 37 °C. .. Afterwards, the DNA template was digested by addition of 4 U of TURBO DNase (Ambion) and incubation for 30 min at 37 °C.

    Pull Down Assay:

    Article Title: eIF4G2 balances its own mRNA translation via a PCBP2-based feedback loop
    Article Snippet: .. RNA pull-down assay RNAs were synthesized using MEGAscript T7 Transcription Kit (Ambion) as suggested by the manufacturer with an addition of 0.75 mM biotin-16-UTP (Jena Bioscience). ..

    Synthesized:

    Article Title: eIF4G2 balances its own mRNA translation via a PCBP2-based feedback loop
    Article Snippet: .. RNA pull-down assay RNAs were synthesized using MEGAscript T7 Transcription Kit (Ambion) as suggested by the manufacturer with an addition of 0.75 mM biotin-16-UTP (Jena Bioscience). ..

    Concentration Assay:

    Article Title: Translational repression of the Drosophila nanos mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch
    Article Snippet: Biotin-16-UTP (Jena Bioscience) and [α-32 P]-UTP were incorporated during transcription at a reduced concentration of UTP. .. For incorporation of a similar number of biotin molecules per RNA, UTP was adjusted according to the number of uridines in the RNA (Luc: 1 mM, 1-AUG: 0.25 mM, SREonly: 0.1 mM) at a constant concentration of biotin-16-UTP (20 µM). ..

    Article Title: Translational repression of the Drosophila nanos mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch
    Article Snippet: RNAs were synthesized with T3 RNA polymerase. .. Biotin-16-UTP (Jena Bioscience) and [α-32 P]-UTP were incorporated during transcription at a reduced concentration of UTP. .. For incorporation of a similar number of biotin molecules per RNA, UTP was adjusted according to the number of uridines in the RNA (Luc: 1 mM, 1-AUG: 0.25 mM, SREonly: 0.1 mM) at a constant concentration of biotin-16-UTP (20 µM).

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  • 94
    Jena Bioscience biotin 16 utp
    Biotin 16 Utp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin 16 utp/product/Jena Bioscience
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin 16 utp - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

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