aminoallyl utp atto 680  (Jena Bioscience)


Bioz Verified Symbol Jena Bioscience is a verified supplier
Bioz Manufacturer Symbol Jena Bioscience manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Name:
    Aminoallyl UTP ATTO 680
    Description:

    Catalog Number:
    NU-821-680
    Price:
    209.54
    Category:
    Nucleotides Nucleosides
    Size:
    10 µl
    Buy from Supplier


    Structured Review

    Jena Bioscience aminoallyl utp atto 680

    https://www.bioz.com/result/aminoallyl utp atto 680/product/Jena Bioscience
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aminoallyl utp atto 680 - by Bioz Stars, 2021-06
    91/100 stars

    Images

    Related Articles

    Synthesized:

    Article Title: Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice
    Article Snippet: Preparation of fluorescently labelled RNAs Overlapping 200 bp fragments corresponding to the mouse mt-Co1 mRNA were synthesized from DNA oligonucleotides and each fragment was cloned into pMA-7-Ar (GeneArt, Life Technologies), flanked at the 5′ end by a T7 promoter sequence and at the 3′ end by an Xma I recognition site. .. Plasmids were linearized by Xma I digestion and fluorescently labelled RNAs were synthesized via direct incorporation of aminoallyl-UTP-ATTO-680 (Jena Bioscience) during in vitro transcription with the MAXIscript T7 kit and purified using NucAway spin columns (Ambion, Life Technologies). .. RNA electrophoretic mobility shift assays Purified TACO1 protein (at equimolar to eightfold in excess concentration relative to the probe) was incubated at room temperature for 30 min with 100 ng μl−1 ATTO-680-labelled RNAs in 10 mM HEPES (pH 8.0), 1 mM EDTA, 50 mM KCl, 2 mM DTT, 0.1 mg ml−1 fatty acid-free bovine serum albumin (BSA) and 0.02% Tween-20.

    In Vitro:

    Article Title: Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice
    Article Snippet: Preparation of fluorescently labelled RNAs Overlapping 200 bp fragments corresponding to the mouse mt-Co1 mRNA were synthesized from DNA oligonucleotides and each fragment was cloned into pMA-7-Ar (GeneArt, Life Technologies), flanked at the 5′ end by a T7 promoter sequence and at the 3′ end by an Xma I recognition site. .. Plasmids were linearized by Xma I digestion and fluorescently labelled RNAs were synthesized via direct incorporation of aminoallyl-UTP-ATTO-680 (Jena Bioscience) during in vitro transcription with the MAXIscript T7 kit and purified using NucAway spin columns (Ambion, Life Technologies). .. RNA electrophoretic mobility shift assays Purified TACO1 protein (at equimolar to eightfold in excess concentration relative to the probe) was incubated at room temperature for 30 min with 100 ng μl−1 ATTO-680-labelled RNAs in 10 mM HEPES (pH 8.0), 1 mM EDTA, 50 mM KCl, 2 mM DTT, 0.1 mg ml−1 fatty acid-free bovine serum albumin (BSA) and 0.02% Tween-20.

    Article Title: Structural basis for the RNA-guided ribonuclease activity of CRISPR-Cas13d
    Article Snippet: In vitro transcription was performed using the Hiscribe T7 High yield RNA synthesis kit (New England Biolabs) at 31°C for 12 hours. .. For in vitro cleavage reactions, targets were body-labeled through incorporation of Aminoallyl-UTP-ATTO-680 (Jena Biosciences) during the in vitro transcription. crRNAs and short competitors were purified using RNAclean Agencourt AMPure XP beads (Beckman Coulter) with addition of 50% volume of isopropanol for retention of small RNAs. .. Longer targets and arrays were purified with the MEGAclear Transcription Clean-Up Kit (Thermo Fisher) and frozen at −80°C.

    Article Title: Drosophila PTB promotes formation of high-order RNP particles and represses oskar translation
    Article Snippet: The MS2-binding protein—MBP-expressing plasmid ( ) was a gift from Reinhard Lührmann. .. RNA transcripts were fluorescently labeled with ATTO-680 Aminoallyl-UTP (Jena Bioscience) via in vitro transcription (T7 megascript kit; Ambion). .. Full-length RNA molecules were gel-purified and incubated with different amounts of recombinant MBP-PTB in the presence of 20 mM Tris-HCl (pH 8.0), 60 mM NaCl, 3 mM MgCl2 , 1 mM DTT, 8% glycerol, 5 μM tRNA during 15 min at 4°C.

    Purification:

    Article Title: Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice
    Article Snippet: Preparation of fluorescently labelled RNAs Overlapping 200 bp fragments corresponding to the mouse mt-Co1 mRNA were synthesized from DNA oligonucleotides and each fragment was cloned into pMA-7-Ar (GeneArt, Life Technologies), flanked at the 5′ end by a T7 promoter sequence and at the 3′ end by an Xma I recognition site. .. Plasmids were linearized by Xma I digestion and fluorescently labelled RNAs were synthesized via direct incorporation of aminoallyl-UTP-ATTO-680 (Jena Bioscience) during in vitro transcription with the MAXIscript T7 kit and purified using NucAway spin columns (Ambion, Life Technologies). .. RNA electrophoretic mobility shift assays Purified TACO1 protein (at equimolar to eightfold in excess concentration relative to the probe) was incubated at room temperature for 30 min with 100 ng μl−1 ATTO-680-labelled RNAs in 10 mM HEPES (pH 8.0), 1 mM EDTA, 50 mM KCl, 2 mM DTT, 0.1 mg ml−1 fatty acid-free bovine serum albumin (BSA) and 0.02% Tween-20.

    Article Title: Structural basis for the RNA-guided ribonuclease activity of CRISPR-Cas13d
    Article Snippet: In vitro transcription was performed using the Hiscribe T7 High yield RNA synthesis kit (New England Biolabs) at 31°C for 12 hours. .. For in vitro cleavage reactions, targets were body-labeled through incorporation of Aminoallyl-UTP-ATTO-680 (Jena Biosciences) during the in vitro transcription. crRNAs and short competitors were purified using RNAclean Agencourt AMPure XP beads (Beckman Coulter) with addition of 50% volume of isopropanol for retention of small RNAs. .. Longer targets and arrays were purified with the MEGAclear Transcription Clean-Up Kit (Thermo Fisher) and frozen at −80°C.

    Electrophoretic Mobility Shift Assay:

    Article Title: Novel mutations in the ferritin-L iron-responsive element that only mildly impair IRP binding cause hereditary hyperferritinaemia cataract syndrome
    Article Snippet: DNA templates were linearized with XbaI and used for in vitro transcription. .. Electrophoretic mobility shift assay (EMSA) EMSAs were performed with a new non-radiolabeled method ([ ], and manuscript in preparation) using fluorescently labeled probes with Aminoallyl-UTP-ATTO-680 (Jena Bioscience). .. For direct and competitive EMSAs we include proper controls such as the +39ΔC deletion construct (positive control), the Milano +36 C > G mutation [ ], a variation at the same position as the Badalona +36C > U mutation and the Torino +29C > G mutation [ ], the counterpart of the Heidelberg +52 G > C change.

    Labeling:

    Article Title: Novel mutations in the ferritin-L iron-responsive element that only mildly impair IRP binding cause hereditary hyperferritinaemia cataract syndrome
    Article Snippet: DNA templates were linearized with XbaI and used for in vitro transcription. .. Electrophoretic mobility shift assay (EMSA) EMSAs were performed with a new non-radiolabeled method ([ ], and manuscript in preparation) using fluorescently labeled probes with Aminoallyl-UTP-ATTO-680 (Jena Bioscience). .. For direct and competitive EMSAs we include proper controls such as the +39ΔC deletion construct (positive control), the Milano +36 C > G mutation [ ], a variation at the same position as the Badalona +36C > U mutation and the Torino +29C > G mutation [ ], the counterpart of the Heidelberg +52 G > C change.

    Article Title: Transcriptome engineering with RNA-targeting Type VI-D CRISPR effectors
    Article Snippet: The oligo anneal and PCR templates were in vitro transcribed with the Hiscribe T7 High Yield RNA Synthesis kit (New England Biolabs) at 31°C for 12 hours. .. For labeled targets, fluorescently labelled aminoallyl-UTP atto 680 (Jena Biosciences) was additionally added at 2 mM. .. Guide RNAs were purified with RNA-grade Agencourt AMPure XP beads (Beckman Coulter) and arrays and targets were purified with MEGAclear Transcription Clean-Up Kit (Thermo Fisher) and frozen at −80°C.

    Article Title: Drosophila PTB promotes formation of high-order RNP particles and represses oskar translation
    Article Snippet: The MS2-binding protein—MBP-expressing plasmid ( ) was a gift from Reinhard Lührmann. .. RNA transcripts were fluorescently labeled with ATTO-680 Aminoallyl-UTP (Jena Bioscience) via in vitro transcription (T7 megascript kit; Ambion). .. Full-length RNA molecules were gel-purified and incubated with different amounts of recombinant MBP-PTB in the presence of 20 mM Tris-HCl (pH 8.0), 60 mM NaCl, 3 mM MgCl2 , 1 mM DTT, 8% glycerol, 5 μM tRNA during 15 min at 4°C.

    Article Title: Subcellular localization and RNP formation of IGF2BPs (IGF2 mRNA-binding proteins) is modulated by distinct RNA-binding domains.
    Article Snippet: .. The IGF2 mRNA-binding protein family (IGF2BPs) directs the cytoplasmic fate of various target mRNAs and controls essential cellular functions. .. The IGF2 mRNA-binding protein family (IGF2BPs) directs the cytoplasmic fate of various target mRNAs and controls essential cellular functions.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Jena Bioscience aminoallyl utp atto 680
    Aminoallyl Utp Atto 680, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aminoallyl utp atto 680/product/Jena Bioscience
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aminoallyl utp atto 680 - by Bioz Stars, 2021-06
    91/100 stars
      Buy from Supplier

    Image Search Results