biological safety cabinet  (NuAire)


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    Name:
    LabGard ES Bench Top Class I Containment Ventilated Enclosure
    Description:
    The LabGard Containment Ventilated Enclosure CVE Model NU 813 is offered as a Class I Containment Enclosure equipped with a HEPA filter In the Class I configuration the NU 813 provides personnel protection for handling low risk aerosols and particulate matter Room air enters the Enclosure flowing past the operator into the work access opening providing an air barrier which minimizes contaminants generated within the enclosure work zone from migrating into the room The air flows through the enclosure carrying contaminants with it exiting through a HEPA filter
    Catalog Number:
    NU-813
    Product Aliases:
    class I, biosafety cabinet, biological safety cabinet, bsc, personnel protection, containment ventilated enclosure, cve
    Price:
    None
    Size:
    3 4 6 foot 0 9 1 2 1 8 meter
    Category:
    Biosafety Cabinet
    Buy from Supplier


    Structured Review

    NuAire biological safety cabinet
    LabGard ES Bench Top Class I Containment Ventilated Enclosure
    The LabGard Containment Ventilated Enclosure CVE Model NU 813 is offered as a Class I Containment Enclosure equipped with a HEPA filter In the Class I configuration the NU 813 provides personnel protection for handling low risk aerosols and particulate matter Room air enters the Enclosure flowing past the operator into the work access opening providing an air barrier which minimizes contaminants generated within the enclosure work zone from migrating into the room The air flows through the enclosure carrying contaminants with it exiting through a HEPA filter
    https://www.bioz.com/result/biological safety cabinet/product/NuAire
    Average 97 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    biological safety cabinet - by Bioz Stars, 2019-08
    97/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Generation of a novel monoclonal antibody that recognizes the alpha (α)-amidated isoform of a valine residue
    Article Snippet: Mice were killed by cervical dislocation; splenocytes were collected under aseptic conditions (Biological Safety Cabinet, Nuaire, Class II Type A/B3) and were promptly fused with the murine myeloma Sp2/0 cells (kindly donated by Dr. Pascal Herion, Instituto de Investigaciones Biomedicas, UNAM, Mexico) using 50 % polyethylene glycol 4000 (Gibco)/PBS (pH 7.4) according to standard cell fusion protocols as described previously [ , ]. .. The murine myeloma cell line Sp2/0 is a variant line derived from the fusion of MOPC-21 and BALB/c mouse splenocytes [ , ]; it is frequently used for the generation of stable hybridomas due to its high growth rate, rapid replication rate, high capacity for cell fusion, and cloning efficiency [ , ].

    Centrifugation:

    Article Title: Characterization of Verotoxin-Encoding Phages from Escherichia coli O103:H2 Strains of Bovine and Human Origins
    Article Snippet: Following incubation at 37°C with shaking at 200 rpm for 3 to 4 h , the bacteria were pelleted by centrifugation, suspended in 5 ml of 0.01 M CaCl2 , and transferred to a 50-ml sterile glass petri dish. .. The dish was placed on a black surface 40 cm below a UV light (G3OT8; GE) in a biological safety cabinet (NuAire Inc., Plymouth, MN).

    Article Title: Inactivation of Pure Bacterial Biofilms by Impaction of Aerosolized Consumer Products Containing Nanoparticulate Metals
    Article Snippet: Agar plates were then poured to have a final volume of 20 mL per plate and left to solidify and dry overnight in a biological safety cabinet (Nuaire, Class 2, Type A2, Plymouth, MN) at room temperature. .. Agar plates were then poured to have a final volume of 20 mL per plate and left to solidify and dry overnight in a biological safety cabinet (Nuaire, Class 2, Type A2, Plymouth, MN) at room temperature.

    Neutralization:

    Article Title: Fate of Salmonella enterica and Enterohemorrhagic Escherichia coli Cells Artificially Internalized into Vegetable Seeds during Germination
    Article Snippet: Residual chlorine on vegetable seeds was neutralized with 20 ml Dey-Engley neutralization broth (BD) for 10 min. Disinfected seeds were rinsed twice, each with 20 ml sterilized distilled water, and were vigorously vortexed for 20 s. Vacuum infiltration was performed according to a procedure previously described by Darrasse et al. ( ) with modifications. .. The draw and release of vacuum were repeated three times , and inoculated vegetable seeds were collected and dried overnight in a biological safety cabinet (class II type A/B 3; Nuaire, Plymouth, MN).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Generation of a novel monoclonal antibody that recognizes the alpha (α)-amidated isoform of a valine residue
    Article Snippet: Hyperimmune mice displaying high antisera titers based on ELISA received an intraperitoneal pre-fusion booster of 50 μg of the KLH-Val-CONH2 immunoconjugate in 200 μL of PBS (pH 7.4) in the absence of adjuvant 7 days before lymphoid cells were harvested [ , ]. .. Mice were killed by cervical dislocation; splenocytes were collected under aseptic conditions (Biological Safety Cabinet, Nuaire, Class II Type A/B3) and were promptly fused with the murine myeloma Sp2/0 cells (kindly donated by Dr. Pascal Herion, Instituto de Investigaciones Biomedicas, UNAM, Mexico) using 50 % polyethylene glycol 4000 (Gibco)/PBS (pH 7.4) according to standard cell fusion protocols as described previously [ , ].

    Ex Vivo:

    Article Title: Pulsatile ex vivo perfusion of human saphenous vein grafts under controlled pressure conditions increases MMP-2 expression
    Article Snippet: Paragraph title: Ex vivo perfusion system ... The vessel mounting procedure is carried out under a biological safety cabinet (NuAire, Plymouth, MN).

    Incubation:

    Article Title: Characterization of Verotoxin-Encoding Phages from Escherichia coli O103:H2 Strains of Bovine and Human Origins
    Article Snippet: Following incubation at 37°C with shaking at 200 rpm for 3 to 4 h , the bacteria were pelleted by centrifugation, suspended in 5 ml of 0.01 M CaCl2 , and transferred to a 50-ml sterile glass petri dish. .. The dish was placed on a black surface 40 cm below a UV light (G3OT8; GE) in a biological safety cabinet (NuAire Inc., Plymouth, MN).

    Article Title: Formulation, Characterization and Pulmonary Deposition of Nebulized Celecoxib Encapsulated Nanostructured Lipid Carriers
    Article Snippet: A549 cells (one million in 15 ml of medium per petri plate) was plated in petri plates was placed on stage 5 of the viable impactor and exposed to the Cxb-NLC formulation for 2 min. After the exposure, the petri plate was taken out from the impactor, covered with sterile aluminum lids provided with the petri plate (Graseby Andersen, Smyrna, GA) and incubated at 37 °C for 72 hours. .. Since all the operations were performed under biological safety cabinet (Class II, Type A/B3, NuAire, Inc., Plymouth, MN) using established tissue culture precautions, sterility can be maintained throughout the incubation period. .. At the end of incubation, the medium in the petri plate was discarded and the cells were rinsed three times with sterile PBS, and detached by adding trypsin.

    Article Title: Inactivation of Pure Bacterial Biofilms by Impaction of Aerosolized Consumer Products Containing Nanoparticulate Metals
    Article Snippet: Agar plates were then poured to have a final volume of 20 mL per plate and left to solidify and dry overnight in a biological safety cabinet (Nuaire, Class 2, Type A2, Plymouth, MN) at room temperature. .. Agar plates were then poured to have a final volume of 20 mL per plate and left to solidify and dry overnight in a biological safety cabinet (Nuaire, Class 2, Type A2, Plymouth, MN) at room temperature.

    HAT Assay:

    Article Title: Generation of a novel monoclonal antibody that recognizes the alpha (α)-amidated isoform of a valine residue
    Article Snippet: Mice were killed by cervical dislocation; splenocytes were collected under aseptic conditions (Biological Safety Cabinet, Nuaire, Class II Type A/B3) and were promptly fused with the murine myeloma Sp2/0 cells (kindly donated by Dr. Pascal Herion, Instituto de Investigaciones Biomedicas, UNAM, Mexico) using 50 % polyethylene glycol 4000 (Gibco)/PBS (pH 7.4) according to standard cell fusion protocols as described previously [ , ]. .. The murine myeloma cell line Sp2/0 is a variant line derived from the fusion of MOPC-21 and BALB/c mouse splenocytes [ , ]; it is frequently used for the generation of stable hybridomas due to its high growth rate, rapid replication rate, high capacity for cell fusion, and cloning efficiency [ , ].

    Modification:

    Article Title: Characterization of Verotoxin-Encoding Phages from Escherichia coli O103:H2 Strains of Bovine and Human Origins
    Article Snippet: A loopful of an overnight broth culture of VTEC O103:H2 was added to a 250-ml baffled flask (Bellco, Vineland, NJ) containing 45 ml of modified Luria-Bertani (LB) broth prepared with 2.5 g/liter of NaCl instead of the usual 5 g and supplemented with 0.01 M CaCl2. .. The dish was placed on a black surface 40 cm below a UV light (G3OT8; GE) in a biological safety cabinet (NuAire Inc., Plymouth, MN).

    Real-time Polymerase Chain Reaction:

    Article Title: Implementation of Novel Design Features for qPCR-Based eDNA Assessment
    Article Snippet: Prior to isolation of total DNA, all sample filters were randomized and processed blind to eliminate bias. .. All further work with filter samples was performed in a biological safety cabinet (NuAire, Plymouth, MN, USA) including total DNA isolation from filters and assembly of the eDNA qPCR assay. .. In all instances of handling individual filter samples, non-serrated stainless steel forceps were used that had been treated sequentially with a bleach (5% w/v sodium hypochlorite) wash, a distilled water rinse, and then wiped dry.

    Sterility:

    Article Title: Formulation, Characterization and Pulmonary Deposition of Nebulized Celecoxib Encapsulated Nanostructured Lipid Carriers
    Article Snippet: A549 cells (one million in 15 ml of medium per petri plate) was plated in petri plates was placed on stage 5 of the viable impactor and exposed to the Cxb-NLC formulation for 2 min. After the exposure, the petri plate was taken out from the impactor, covered with sterile aluminum lids provided with the petri plate (Graseby Andersen, Smyrna, GA) and incubated at 37 °C for 72 hours. .. Since all the operations were performed under biological safety cabinet (Class II, Type A/B3, NuAire, Inc., Plymouth, MN) using established tissue culture precautions, sterility can be maintained throughout the incubation period. .. At the end of incubation, the medium in the petri plate was discarded and the cells were rinsed three times with sterile PBS, and detached by adding trypsin.

    Flow Cytometry:

    Article Title: Protein-inspired antibiotics active against vancomycin- and daptomycin-resistant bacteria
    Article Snippet: Bacto agar (Cat# 214040, BD DIFCO, USA), brain heart infusion broth (Cat# 237500, BD, USA), fetal bovine serum (Cat# 30071.03, HyClone, USA), sodium chloride (Cat# S7653, SIGMA-Aldrich, USA), vancomycin (Cat# V2002, Sigma, USA) and water for injection (WFI) (Tai-Yu, Taiwan). .. Animal cage (Allentown, USA), biokinetic reader (Bio-Tek, USA), biological safety cabinet (NuAire, USA), centrifuge (Eppendrof 5810 R, Germany), laminar flow (Chao-Shin, Taiwan), orbital shaking incubator (Firstek Scientific, Taiwan), Pipetman P100 (Gilson, France), refrigerated incubator (Hotpack, USA) and ultra-low temperature freezer (NuAire, USA). .. Assay # 608100 S. pneumoniae (ATCC® 6301 ™ ), Infected Lung Model. Groups of 10 male specific-pathogen-free ICR mice weighing 22 ± 2 g were used.

    Article Title: Pulsatile ex vivo perfusion of human saphenous vein grafts under controlled pressure conditions increases MMP-2 expression
    Article Snippet: The circuit of the perfusion system is driven by a roller pump ISMATEC S2 (Wertheim, Germany) producing a pulsatile and non-static flow. .. The vessel mounting procedure is carried out under a biological safety cabinet (NuAire, Plymouth, MN).

    Article Title: Formulation, Characterization and Pulmonary Deposition of Nebulized Celecoxib Encapsulated Nanostructured Lipid Carriers
    Article Snippet: To determine the in-vitro cytotoxicity of aerosolized Cxb-NLC formulation, the viable impactor was connected to the Pari LC Star jet nebulizer through an USP throat and operated at a flow rate of 28.3 L/min. .. Since all the operations were performed under biological safety cabinet (Class II, Type A/B3, NuAire, Inc., Plymouth, MN) using established tissue culture precautions, sterility can be maintained throughout the incubation period.

    Dissection:

    Article Title: Antisense Oligonucleotide-mediated Knockdown in Mammary Tumor Organoids
    Article Snippet: Optimal tumor size is ~5–10 mm in diameter 200 Proof ethyl alcohol ( e.g. , UltraPure, catalog number: 200CSPTP) Sterile water Ice Matrigel Growth Factor reduced Basement Membrane Matrix, phenol-red free (Corning, catalog number: 356231) Optional: Tissue-Tek O.C.T. compound (Electron Microscopy Sciences, catalog number: 62550-12) Liquid N2 Cell recovery solution (Corning, catalog number: 354253) 1x DPBS ( e.g. , Thermo Fisher Scientific, Gibco™, catalog number: 14190250) TRIzol (Thermo Fisher Scientific, Invitrogen™, catalog number: 15596018) GlycoBlue (Thermo Fisher Scientific, Invitrogen™, catalog number: AM9516) DNAse I, amplification grade, for cDNA synthesis ( e.g. , Thermo Fisher Scientific, Invitrogen™, catalog number: 18068015) Ethylenediaminetetraacetate acid disodium salt (EDTA) Fetal bovine serum (FBS) ( e.g. , VWR, product number: 1500-500) Gentamicin 50 mg/ml ( e.g. , Lonza, catalog number: 17-528Z) Insulin from bovine pancreas (Sigma-Aldrich, catalog number: I1882-100MG) Trypsin ( e.g. , Mediatech, catalog number: 25-054-CI) Collagenase from Clostridium histolyticum (Sigma-Aldrich, catalog number: C5138-1G) Advanced DMEM/F12 ( e.g. , Thermo Fisher Scientific, Gibco™, catalog number: 12634010) Bovine serum albumin (BSA) ( e.g. , Sigma-Aldrich, catalog number: A2153-100G) DNase I from bovine pancreas for organoid preparation ( e.g. , Sigma-Aldrich, catalog number: D4263-1VL) Pen/Strep ( e.g. , Sigma-Aldrich, catalog numbers: PENNA-100MU and S6501-100G) ITS liquid media supplement 100x (Sigma-Aldrich, catalog number: I3146-5ML) Murine FGF-basic (PeproTech, catalog number: 450-33) Dimethyl sulfoxide (DMSO) ( e.g. , Sigma-Aldrich, catalog number: D2650-5x10ML) Nuclease-free water ( e.g. , Thermo Fisher Scientific, Invitrogen™, catalog number: AM9937) TaqMan Reverse Transcription Kit (Thermo Fisher Scientific, Invitrogen™, catalog number: N8080234) PowerUp SYBR Green Master Mix (Thermo Fischer Scientific, Applied Biosystems™, catalog number: ) Chloroform, purified ( e.g. , Avantor Performance Materials, MACRON, catalog number: 4432-10) Isopropanol, molecular biology grade ( e.g. , Fisher Scientific, catalog number: BP2618500) Collagenase solution (see Recipes) BSA solution (see Recipes) DNase solution (see Recipes) Organoid medium (10 ml, sufficient for 10 wells) (see Recipes) Freezing medium (see Recipes) cDNA Master mix (see Recipes) qPCR Master mix (see Recipes) .. Biological safety cabinet ( e.g ., NuAire) Dissection tools ( e.g., 114.3 mm scissors, Sklar Surgical Instruments, catalog number: 98-104; forceps, Sklar Surgical Instruments, catalog number: 97-751, sterile scalpels, e.g. , Sklar Surgical Instruments, catalog number: 06-3110) Cell culture incubator ( e.g. , Heracell i Copper CO2 incubator, Thermo Fischer Scientific, Thermo Scientific™, model: Heracell™ 150i and 240i, catalog number: 50116050) Shaker with temperature control ( e.g. , Thomas Scientific, catalog number: 1222U12) Manufacturer: Benchmark Scientific, catalog number: H1000-M. .. Centrifuge for 15 and 50 ml tubes ( e.g. , Eppendorf, model: 5804) Centrifuge for 1.5 ml reaction tubes with cooling function ( e.g. , Eppendorf, model: 5427 R) Vacuum suction Micro-pipettes ( e.g. , Gilson, catalog number: F167300, catalog number for Thomas Scientific: 1222N73) Phase-contrast microscope ( e.g ., Nikon) Optional: Cryo-Safe Freeze Controller ( e.g. , SP Scienceware - Bel-Art Products - H-B Instrument, catalog number: F18844-0000) NanoDrop 2000 UV-Vis spectrophotometer (Thermo Fisher Scientific, Thermo Scientific™, model: NanoDrop™ 2000, catalog number: ND-2000) PCR machine ( e.g. , Applied Biosystems Proflex Thermocycler, Thermo Fisher Scientific, Applied Biosystems™, catalog number: 4484073) qPCR machine ( e.g. , Applied Biosystems StepOne Plus Real-Time PCR system, Thermo Fisher Scientific, Thermo Scientific™, catalog number: 4376600) −80 °C freezer ( e.g. , VWR, catalog number: 10160-728) Water bath ( e.g. , PolyScience, catalog number: WB10A11B) Optional: liquid N2 freezers ( e.g. , VWR, catalog number: 82017-934) Refrigerator (4 °C)

    Cell Culture:

    Article Title: Changes in the Proteome of Langat-Infected Ixodes scapularis ISE6 Cells: Metabolic Pathways Associated with Flavivirus Infection
    Article Snippet: Serial IFAs were conducted in parallel as previously described [ ] in 96-well cell culture plates to determine LGTV stock titers. .. To create non-infectious LGTV (UV-LGTV), LGTV p4 stock medium was placed in 48 well cell culture plates and treated with UV radiation at a distance of 11 cm from a standard (12.4 watt) UV lamp in a biological safety cabinet (Nuaire Labgard ES, Plymouth, MN) for 30 second intervals over a five minute period. .. LGTV inactivation was confirmed by blind passage of UV-LGTV on ~2 x 107 ISE6 cells and ~80% confluent BHK15 cells, followed by immunofluorescent and plaque assay as described by Perera et al. [ ] to demonstrate lack of infectivity.

    Article Title: Antisense Oligonucleotide-mediated Knockdown in Mammary Tumor Organoids
    Article Snippet: Optimal tumor size is ~5–10 mm in diameter 200 Proof ethyl alcohol ( e.g. , UltraPure, catalog number: 200CSPTP) Sterile water Ice Matrigel Growth Factor reduced Basement Membrane Matrix, phenol-red free (Corning, catalog number: 356231) Optional: Tissue-Tek O.C.T. compound (Electron Microscopy Sciences, catalog number: 62550-12) Liquid N2 Cell recovery solution (Corning, catalog number: 354253) 1x DPBS ( e.g. , Thermo Fisher Scientific, Gibco™, catalog number: 14190250) TRIzol (Thermo Fisher Scientific, Invitrogen™, catalog number: 15596018) GlycoBlue (Thermo Fisher Scientific, Invitrogen™, catalog number: AM9516) DNAse I, amplification grade, for cDNA synthesis ( e.g. , Thermo Fisher Scientific, Invitrogen™, catalog number: 18068015) Ethylenediaminetetraacetate acid disodium salt (EDTA) Fetal bovine serum (FBS) ( e.g. , VWR, product number: 1500-500) Gentamicin 50 mg/ml ( e.g. , Lonza, catalog number: 17-528Z) Insulin from bovine pancreas (Sigma-Aldrich, catalog number: I1882-100MG) Trypsin ( e.g. , Mediatech, catalog number: 25-054-CI) Collagenase from Clostridium histolyticum (Sigma-Aldrich, catalog number: C5138-1G) Advanced DMEM/F12 ( e.g. , Thermo Fisher Scientific, Gibco™, catalog number: 12634010) Bovine serum albumin (BSA) ( e.g. , Sigma-Aldrich, catalog number: A2153-100G) DNase I from bovine pancreas for organoid preparation ( e.g. , Sigma-Aldrich, catalog number: D4263-1VL) Pen/Strep ( e.g. , Sigma-Aldrich, catalog numbers: PENNA-100MU and S6501-100G) ITS liquid media supplement 100x (Sigma-Aldrich, catalog number: I3146-5ML) Murine FGF-basic (PeproTech, catalog number: 450-33) Dimethyl sulfoxide (DMSO) ( e.g. , Sigma-Aldrich, catalog number: D2650-5x10ML) Nuclease-free water ( e.g. , Thermo Fisher Scientific, Invitrogen™, catalog number: AM9937) TaqMan Reverse Transcription Kit (Thermo Fisher Scientific, Invitrogen™, catalog number: N8080234) PowerUp SYBR Green Master Mix (Thermo Fischer Scientific, Applied Biosystems™, catalog number: ) Chloroform, purified ( e.g. , Avantor Performance Materials, MACRON, catalog number: 4432-10) Isopropanol, molecular biology grade ( e.g. , Fisher Scientific, catalog number: BP2618500) Collagenase solution (see Recipes) BSA solution (see Recipes) DNase solution (see Recipes) Organoid medium (10 ml, sufficient for 10 wells) (see Recipes) Freezing medium (see Recipes) cDNA Master mix (see Recipes) qPCR Master mix (see Recipes) .. Biological safety cabinet ( e.g ., NuAire) Dissection tools ( e.g., 114.3 mm scissors, Sklar Surgical Instruments, catalog number: 98-104; forceps, Sklar Surgical Instruments, catalog number: 97-751, sterile scalpels, e.g. , Sklar Surgical Instruments, catalog number: 06-3110) Cell culture incubator ( e.g. , Heracell i Copper CO2 incubator, Thermo Fischer Scientific, Thermo Scientific™, model: Heracell™ 150i and 240i, catalog number: 50116050) Shaker with temperature control ( e.g. , Thomas Scientific, catalog number: 1222U12) Manufacturer: Benchmark Scientific, catalog number: H1000-M. .. Centrifuge for 15 and 50 ml tubes ( e.g. , Eppendorf, model: 5804) Centrifuge for 1.5 ml reaction tubes with cooling function ( e.g. , Eppendorf, model: 5427 R) Vacuum suction Micro-pipettes ( e.g. , Gilson, catalog number: F167300, catalog number for Thomas Scientific: 1222N73) Phase-contrast microscope ( e.g ., Nikon) Optional: Cryo-Safe Freeze Controller ( e.g. , SP Scienceware - Bel-Art Products - H-B Instrument, catalog number: F18844-0000) NanoDrop 2000 UV-Vis spectrophotometer (Thermo Fisher Scientific, Thermo Scientific™, model: NanoDrop™ 2000, catalog number: ND-2000) PCR machine ( e.g. , Applied Biosystems Proflex Thermocycler, Thermo Fisher Scientific, Applied Biosystems™, catalog number: 4484073) qPCR machine ( e.g. , Applied Biosystems StepOne Plus Real-Time PCR system, Thermo Fisher Scientific, Thermo Scientific™, catalog number: 4376600) −80 °C freezer ( e.g. , VWR, catalog number: 10160-728) Water bath ( e.g. , PolyScience, catalog number: WB10A11B) Optional: liquid N2 freezers ( e.g. , VWR, catalog number: 82017-934) Refrigerator (4 °C)

    Generated:

    Article Title: Stratosphere Conditions Inactivate Bacterial Endospores from a Mars Spacecraft Assembly Facility
    Article Snippet: To supplement the balloon flight experiment, we used another group of coupons to evaluate B. pumilus SAFR-032 spore resistance to artificially generated UVC conditions in the laboratory. .. Within a biological safety cabinet (Labgard Class II, Type A/B3, Model NU-600 Series 24, NuAire, Inc.), a 3-D-printed acrylic UV light-emitting diode (LED) test stand (12.0 × 7.5 × 14.1 cm) held experimental coupons at vertical distances of 1–5 cm from the light source ( ).

    DNA Extraction:

    Article Title: Implementation of Novel Design Features for qPCR-Based eDNA Assessment
    Article Snippet: Prior to isolation of total DNA, all sample filters were randomized and processed blind to eliminate bias. .. All further work with filter samples was performed in a biological safety cabinet (NuAire, Plymouth, MN, USA) including total DNA isolation from filters and assembly of the eDNA qPCR assay. .. In all instances of handling individual filter samples, non-serrated stainless steel forceps were used that had been treated sequentially with a bleach (5% w/v sodium hypochlorite) wash, a distilled water rinse, and then wiped dry.

    Plaque Assay:

    Article Title: Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 2. General Practices
    Article Snippet: Plaque assay for murine norovirus. .. Working safely in your NuAire biological safety cabinet.

    Isolation:

    Article Title: Implementation of Novel Design Features for qPCR-Based eDNA Assessment
    Article Snippet: Paragraph title: Isolation of Total DNA from Filtered Water Samples ... All further work with filter samples was performed in a biological safety cabinet (NuAire, Plymouth, MN, USA) including total DNA isolation from filters and assembly of the eDNA qPCR assay.

    Microelectrode Array:

    Article Title: Effect of UV-C radiation and vapor released from a water hyacinth root absorbent containing bergamot oil to control mold on storage of brown rice
    Article Snippet: The rice was put into a biological safety cabinet (Cellgard, NuAire, USA) to be dried for 10 min Mold spore were allowed to attach to brown rice for 90 min. Next, the absorbent from each of the round disc (ϕ ~ 20 mm) was placed into the Petri dish lid. .. The rice was put into a biological safety cabinet (Cellgard, NuAire, USA) to be dried for 10 min Mold spore were allowed to attach to brown rice for 90 min. Next, the absorbent from each of the round disc (ϕ ~ 20 mm) was placed into the Petri dish lid.

    Microscopy:

    Article Title: A simple and reproducible 96 well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing
    Article Snippet: Acetone (Sigma, cat. no. 34850) Antifungal drugs: Typically fluconazole (Pfizer, Inc.), amphotericin B (Bristol-Myers Squibb) and caspofungin (Merck & Co., Inc.) as representatives of the three main classes of antifungal agents most frequently used in the clinics: azoles, polyenes and echinocandins respectively. .. Autoclave (Sanyo) Disposable plastic vacuum filter system, 500 ml (Corning, cat no. 430769) Petri dishes (Fisher, cat no. 08-757-12) 15 ml conical centrifuge tubes (Corning, cat no. 430790) 50 ml conical centrifuge tubes (Corning, cat no. 430828) Erlenmeyer flasks (Corning) Biological safety cabinet (Nuaire) Vortex mixer (Fisher) Orbital shaker (New Brunswick) Centrifuge (Eppendorf) Hemacytometer (Hausser Scientific) Microscope (Fisher) 96 well microtiter plates: Polystyrene, flat-bottomed, tissue culture treated (Corning, cat. no. 3595). .. Parafilm Multichannel pipette and tips (Eppendorf) Incubator (Fisher) Inverted Microscope (optional) (Fisher) Microtiter plate reader (Bio-Rad)

    Mouse Assay:

    Article Title: Generation of a novel monoclonal antibody that recognizes the alpha (α)-amidated isoform of a valine residue
    Article Snippet: Hyperimmune mice displaying high antisera titers based on ELISA received an intraperitoneal pre-fusion booster of 50 μg of the KLH-Val-CONH2 immunoconjugate in 200 μL of PBS (pH 7.4) in the absence of adjuvant 7 days before lymphoid cells were harvested [ , ]. .. Mice were killed by cervical dislocation; splenocytes were collected under aseptic conditions (Biological Safety Cabinet, Nuaire, Class II Type A/B3) and were promptly fused with the murine myeloma Sp2/0 cells (kindly donated by Dr. Pascal Herion, Instituto de Investigaciones Biomedicas, UNAM, Mexico) using 50 % polyethylene glycol 4000 (Gibco)/PBS (pH 7.4) according to standard cell fusion protocols as described previously [ , ]. .. The murine myeloma cell line Sp2/0 is a variant line derived from the fusion of MOPC-21 and BALB/c mouse splenocytes [ , ]; it is frequently used for the generation of stable hybridomas due to its high growth rate, rapid replication rate, high capacity for cell fusion, and cloning efficiency [ , ].

    Liquid Chromatography:

    Article Title: Formulation, Characterization and Pulmonary Deposition of Nebulized Celecoxib Encapsulated Nanostructured Lipid Carriers
    Article Snippet: To determine the in-vitro cytotoxicity of aerosolized Cxb-NLC formulation, the viable impactor was connected to the Pari LC Star jet nebulizer through an USP throat and operated at a flow rate of 28.3 L/min. .. Since all the operations were performed under biological safety cabinet (Class II, Type A/B3, NuAire, Inc., Plymouth, MN) using established tissue culture precautions, sterility can be maintained throughout the incubation period.

    Irradiation:

    Article Title: Effect of UV-C radiation and vapor released from a water hyacinth root absorbent containing bergamot oil to control mold on storage of brown rice
    Article Snippet: After compressing dried root (10 ± 5 g) in a stainless tube into a round disc (20 mm diameter and 3 mm long), the absorbent materials obtained were then irradiated with UV-C for 10, 15, and 20 min. .. The rice was put into a biological safety cabinet (Cellgard, NuAire, USA) to be dried for 10 min Mold spore were allowed to attach to brown rice for 90 min. Next, the absorbent from each of the round disc (ϕ ~ 20 mm) was placed into the Petri dish lid.

    Article Title: Characterization of Verotoxin-Encoding Phages from Escherichia coli O103:H2 Strains of Bovine and Human Origins
    Article Snippet: Phage induction was carried out by UV irradiation of the VTEC O103:H2 strains according to the method of O'Brien et al. ( ). .. The dish was placed on a black surface 40 cm below a UV light (G3OT8; GE) in a biological safety cabinet (NuAire Inc., Plymouth, MN).

    In Vitro:

    Article Title: Formulation, Characterization and Pulmonary Deposition of Nebulized Celecoxib Encapsulated Nanostructured Lipid Carriers
    Article Snippet: Paragraph title: 2.10 In-vitro cytotoxicity of aerosolized Cxb-NLC against A549 Cells ... Since all the operations were performed under biological safety cabinet (Class II, Type A/B3, NuAire, Inc., Plymouth, MN) using established tissue culture precautions, sterility can be maintained throughout the incubation period.

    Concentration Assay:

    Article Title: Generation of a novel monoclonal antibody that recognizes the alpha (α)-amidated isoform of a valine residue
    Article Snippet: Mice were killed by cervical dislocation; splenocytes were collected under aseptic conditions (Biological Safety Cabinet, Nuaire, Class II Type A/B3) and were promptly fused with the murine myeloma Sp2/0 cells (kindly donated by Dr. Pascal Herion, Instituto de Investigaciones Biomedicas, UNAM, Mexico) using 50 % polyethylene glycol 4000 (Gibco)/PBS (pH 7.4) according to standard cell fusion protocols as described previously [ , ]. .. The murine myeloma cell line Sp2/0 is a variant line derived from the fusion of MOPC-21 and BALB/c mouse splenocytes [ , ]; it is frequently used for the generation of stable hybridomas due to its high growth rate, rapid replication rate, high capacity for cell fusion, and cloning efficiency [ , ].

    Article Title: Effect of UV-C radiation and vapor released from a water hyacinth root absorbent containing bergamot oil to control mold on storage of brown rice
    Article Snippet: The rice was put into a biological safety cabinet (Cellgard, NuAire, USA) to be dried for 10 min Mold spore were allowed to attach to brown rice for 90 min. Next, the absorbent from each of the round disc (ϕ ~ 20 mm) was placed into the Petri dish lid. .. Then, each count of the A. flavus was done by using MEA to count the colonies (Suhem et al. ).

    Article Title: Inactivation of Pure Bacterial Biofilms by Impaction of Aerosolized Consumer Products Containing Nanoparticulate Metals
    Article Snippet: Paragraph title: Minimal Inhibitory Concentration (MIC) Tests ... Agar plates were then poured to have a final volume of 20 mL per plate and left to solidify and dry overnight in a biological safety cabinet (Nuaire, Class 2, Type A2, Plymouth, MN) at room temperature.

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    NuAire biological safety cabinet
    Biological Safety Cabinet, supplied by NuAire, used in various techniques. Bioz Stars score: 97/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biological safety cabinet/product/NuAire
    Average 97 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    biological safety cabinet - by Bioz Stars, 2019-08
    97/100 stars
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