5 propargylamino dctp cy5  (Jena Bioscience)


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  • 94
    Name:
    5 Propargylamino dCTP Cy5
    Description:

    Catalog Number:
    NU-809-CY5-L
    Price:
    332.92
    Category:
    Nucleotides Nucleosides
    Size:
    5 x 10 µl
    Buy from Supplier


    Structured Review

    Jena Bioscience 5 propargylamino dctp cy5
    Scheme for selective fluorescent labeling of single-stranded PT modifications. ( A ) Upon iodine-induced strand cleavage and subsequent dephosphorylation with shrimp alkaline phosphatase, the single-stranded PT at 5′-C PS CA-3′ is converted to a DNA nick with a free 3′-OH, which is filled by Taq DNA polymerase-mediated sequential incorporation of <t>5-propargylamino-dCTP-Cy5</t> and dideoxyATP. ( B ) Selected images of the 15-kb-C PS CA molecules labeled with Cy5 (shown in violet) present close to the left end. The DNA backbone is stained with YOYO-1 (green); scale bar: 2.5 μm.

    https://www.bioz.com/result/5 propargylamino dctp cy5/product/Jena Bioscience
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    5 propargylamino dctp cy5 - by Bioz Stars, 2021-06
    94/100 stars

    Images

    1) Product Images from "Single-molecule optical mapping of the distribution of DNA phosphorothioate epigenetics"

    Article Title: Single-molecule optical mapping of the distribution of DNA phosphorothioate epigenetics

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkab169

    Scheme for selective fluorescent labeling of single-stranded PT modifications. ( A ) Upon iodine-induced strand cleavage and subsequent dephosphorylation with shrimp alkaline phosphatase, the single-stranded PT at 5′-C PS CA-3′ is converted to a DNA nick with a free 3′-OH, which is filled by Taq DNA polymerase-mediated sequential incorporation of 5-propargylamino-dCTP-Cy5 and dideoxyATP. ( B ) Selected images of the 15-kb-C PS CA molecules labeled with Cy5 (shown in violet) present close to the left end. The DNA backbone is stained with YOYO-1 (green); scale bar: 2.5 μm.
    Figure Legend Snippet: Scheme for selective fluorescent labeling of single-stranded PT modifications. ( A ) Upon iodine-induced strand cleavage and subsequent dephosphorylation with shrimp alkaline phosphatase, the single-stranded PT at 5′-C PS CA-3′ is converted to a DNA nick with a free 3′-OH, which is filled by Taq DNA polymerase-mediated sequential incorporation of 5-propargylamino-dCTP-Cy5 and dideoxyATP. ( B ) Selected images of the 15-kb-C PS CA molecules labeled with Cy5 (shown in violet) present close to the left end. The DNA backbone is stained with YOYO-1 (green); scale bar: 2.5 μm.

    Techniques Used: Labeling, De-Phosphorylation Assay, Staining

    2) Product Images from "Single-molecule optical mapping of the distribution of DNA phosphorothioate epigenetics"

    Article Title: Single-molecule optical mapping of the distribution of DNA phosphorothioate epigenetics

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkab169

    Scheme for selective fluorescent labeling of single-stranded PT modifications. ( A ) Upon iodine-induced strand cleavage and subsequent dephosphorylation with shrimp alkaline phosphatase, the single-stranded PT at 5′-C PS CA-3′ is converted to a DNA nick with a free 3′-OH, which is filled by Taq DNA polymerase-mediated sequential incorporation of 5-propargylamino-dCTP-Cy5 and dideoxyATP. ( B ) Selected images of the 15-kb-C PS CA molecules labeled with Cy5 (shown in violet) present close to the left end. The DNA backbone is stained with YOYO-1 (green); scale bar: 2.5 μm.
    Figure Legend Snippet: Scheme for selective fluorescent labeling of single-stranded PT modifications. ( A ) Upon iodine-induced strand cleavage and subsequent dephosphorylation with shrimp alkaline phosphatase, the single-stranded PT at 5′-C PS CA-3′ is converted to a DNA nick with a free 3′-OH, which is filled by Taq DNA polymerase-mediated sequential incorporation of 5-propargylamino-dCTP-Cy5 and dideoxyATP. ( B ) Selected images of the 15-kb-C PS CA molecules labeled with Cy5 (shown in violet) present close to the left end. The DNA backbone is stained with YOYO-1 (green); scale bar: 2.5 μm.

    Techniques Used: Labeling, De-Phosphorylation Assay, Staining

    Related Articles

    Incubation:

    Article Title: Single-molecule optical mapping of the distribution of DNA phosphorothioate epigenetics
    Article Snippet: The purified DNA products were next mixed with 1 μl of shrimp alkaline phosphatase (New England Biolabs) and 5 μl of NEB CutSmart buffer to remove 3′-phosphates arising from iodine cleavage. .. After incubation at 37°C, the reaction was terminated by heating at 75°C for an additional 10 min. To fill PT-converted nicks, DNA samples were incubated with 1 μl of Taq DNA polymerase (New England Biolabs), 100 nM ddATP, 100 nM ddTTP, 100 nM ddGTP, 100 nM 5-propargylamino-dCTP-Cy5 (Jena Bioscience) and 5 μl of ThermoPol buffer (20 mM Tris-HCl (pH 8.8), 10 mM (NH4 )2 SO4 , 10 mM KCl, 2 mM MgSO4 and 0.1% Triton® X-100) in a total volume of 50 μl. ..

    Polymerase Chain Reaction:

    Article Title: Simple and cost-effective fluorescent labeling of 5-hydroxymethylcytosine.
    Article Snippet: The nucleobase 5-hydroxymethylcytosine (5-hmC), a modified form of cytosine, is an important epigenetic mark related to regulation of gene expression. .. The nucleobase 5-hydroxymethylcytosine (5-hmC), a modified form of cytosine, is an important epigenetic mark related to regulation of gene expression. ..

    Article Title: Spectroscopic quantification of 5-hydroxymethylcytosine in genomic DNA.
    Article Snippet: .. 5-Hydroxymethylcytosine (5hmC), a modified form of the DNA base cytosine, is an important epigenetic mark linked to regulation of gene expression in development, and tumorigenesis. .. 5-Hydroxymethylcytosine (5hmC), a modified form of the DNA base cytosine, is an important epigenetic mark linked to regulation of gene expression in development, and tumorigenesis.

    Lambda DNA Preparation:

    Article Title: Simple and cost-effective fluorescent labeling of 5-hydroxymethylcytosine.
    Article Snippet: The nucleobase 5-hydroxymethylcytosine (5-hmC), a modified form of cytosine, is an important epigenetic mark related to regulation of gene expression. .. The nucleobase 5-hydroxymethylcytosine (5-hmC), a modified form of cytosine, is an important epigenetic mark related to regulation of gene expression. ..

    Labeling:

    Article Title: Spectroscopic quantification of 5-hydroxymethylcytosine in genomic DNA.
    Article Snippet: .. 5-Hydroxymethylcytosine (5hmC), a modified form of the DNA base cytosine, is an important epigenetic mark linked to regulation of gene expression in development, and tumorigenesis. .. 5-Hydroxymethylcytosine (5hmC), a modified form of the DNA base cytosine, is an important epigenetic mark linked to regulation of gene expression in development, and tumorigenesis.

    Sequencing:

    Article Title: Spectroscopic quantification of 5-hydroxymethylcytosine in genomic DNA.
    Article Snippet: .. 5-Hydroxymethylcytosine (5hmC), a modified form of the DNA base cytosine, is an important epigenetic mark linked to regulation of gene expression in development, and tumorigenesis. .. 5-Hydroxymethylcytosine (5hmC), a modified form of the DNA base cytosine, is an important epigenetic mark linked to regulation of gene expression in development, and tumorigenesis.

    other:

    Article Title: Single-molecule optical mapping of the distribution of DNA phosphorothioate epigenetics
    Article Snippet: For 5-propargylamino-dCTP-Cy5, a 640-nm laser was used for excitation.

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  • 94
    Jena Bioscience 5 propargylamino dctp cy5
    Scheme for selective fluorescent labeling of single-stranded PT modifications. ( A ) Upon iodine-induced strand cleavage and subsequent dephosphorylation with shrimp alkaline phosphatase, the single-stranded PT at 5′-C PS CA-3′ is converted to a DNA nick with a free 3′-OH, which is filled by Taq DNA polymerase-mediated sequential incorporation of <t>5-propargylamino-dCTP-Cy5</t> and dideoxyATP. ( B ) Selected images of the 15-kb-C PS CA molecules labeled with Cy5 (shown in violet) present close to the left end. The DNA backbone is stained with YOYO-1 (green); scale bar: 2.5 μm.
    5 Propargylamino Dctp Cy5, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 propargylamino dctp cy5/product/Jena Bioscience
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    5 propargylamino dctp cy5 - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier


    Image Search Results


    Scheme for selective fluorescent labeling of single-stranded PT modifications. ( A ) Upon iodine-induced strand cleavage and subsequent dephosphorylation with shrimp alkaline phosphatase, the single-stranded PT at 5′-C PS CA-3′ is converted to a DNA nick with a free 3′-OH, which is filled by Taq DNA polymerase-mediated sequential incorporation of 5-propargylamino-dCTP-Cy5 and dideoxyATP. ( B ) Selected images of the 15-kb-C PS CA molecules labeled with Cy5 (shown in violet) present close to the left end. The DNA backbone is stained with YOYO-1 (green); scale bar: 2.5 μm.

    Journal: Nucleic Acids Research

    Article Title: Single-molecule optical mapping of the distribution of DNA phosphorothioate epigenetics

    doi: 10.1093/nar/gkab169

    Figure Lengend Snippet: Scheme for selective fluorescent labeling of single-stranded PT modifications. ( A ) Upon iodine-induced strand cleavage and subsequent dephosphorylation with shrimp alkaline phosphatase, the single-stranded PT at 5′-C PS CA-3′ is converted to a DNA nick with a free 3′-OH, which is filled by Taq DNA polymerase-mediated sequential incorporation of 5-propargylamino-dCTP-Cy5 and dideoxyATP. ( B ) Selected images of the 15-kb-C PS CA molecules labeled with Cy5 (shown in violet) present close to the left end. The DNA backbone is stained with YOYO-1 (green); scale bar: 2.5 μm.

    Article Snippet: After incubation at 37°C, the reaction was terminated by heating at 75°C for an additional 10 min. To fill PT-converted nicks, DNA samples were incubated with 1 μl of Taq DNA polymerase (New England Biolabs), 100 nM ddATP, 100 nM ddTTP, 100 nM ddGTP, 100 nM 5-propargylamino-dCTP-Cy5 (Jena Bioscience) and 5 μl of ThermoPol buffer (20 mM Tris-HCl (pH 8.8), 10 mM (NH4 )2 SO4 , 10 mM KCl, 2 mM MgSO4 and 0.1% Triton® X-100) in a total volume of 50 μl.

    Techniques: Labeling, De-Phosphorylation Assay, Staining