biotinylated nucleotides  (Jena Bioscience)


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    Name:
    Biotin 16 dCTP
    Description:
    Biotin 16 dCTP is enzymatically incorporated into DNA cDNA as substitute for its natural counterpart dCTP The resulting Biotin labeled DNA cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase HRP alkaline phosphatase AP a fluorescent dye or agarose magnetic beads Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 16 atom linker attached to the C5 position of cytidine Recommended Biotin 16 dCTP dCTP ratio for PCR 50 Biotin 16 dCTP 50 dCTP Please note The optimal final concentration of Biotin 16 dCTP may very depending on the application and assay conditions For optimal product yields and high incorporation rates an individual optimization of the Biotin 16 dCTP dTTP ratio is recommended
    Catalog Number:
    nu-809-bio16-l
    Price:
    234.09
    Applications:
    Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I [1,2] - Primer Extension with Klenow exo- [3] - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [4] - Reverse Transcription with MMLV Reverse Transcriptase [5]
    Purity:
    ≥ 95 % (HPLC)
    Category:
    Nucleotides Nucleosides
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    Structured Review

    Jena Bioscience biotinylated nucleotides
    Biotin 16 dCTP is enzymatically incorporated into DNA cDNA as substitute for its natural counterpart dCTP The resulting Biotin labeled DNA cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase HRP alkaline phosphatase AP a fluorescent dye or agarose magnetic beads Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 16 atom linker attached to the C5 position of cytidine Recommended Biotin 16 dCTP dCTP ratio for PCR 50 Biotin 16 dCTP 50 dCTP Please note The optimal final concentration of Biotin 16 dCTP may very depending on the application and assay conditions For optimal product yields and high incorporation rates an individual optimization of the Biotin 16 dCTP dTTP ratio is recommended
    https://www.bioz.com/result/biotinylated nucleotides/product/Jena Bioscience
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    biotinylated nucleotides - by Bioz Stars, 2020-08
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: .. In order to increase SAPE signal, we tested addition of Biotin-16-dCTP (Jena Bioscience, Germany) to the PCR reaction mix in a ratio of 1:1 to the dCTPs included within the original EliZyme master mix. .. Hybridization to microspheres and MAGPIX analysis The MOL-PCR products were hybridized to a particular magnetic microsphere set using xTAG sequences complementary to anti-TAG sequences covalently linked to the microspheres’ surface.

    Lambda DNA Preparation:

    Article Title: Blocking the RecA activity and SOS-response in bacteria with a short α-helical peptide
    Article Snippet: .. First channel contained 50 fM of streptavidin-coated polystyrene beads (2.1 μm, Spherotech), second channel—5 pM of Lambda DNA (New England Biolabs) end labeled with biotinylated nucleotides (Biotin-16-dCTP, Jena Bioscience) using Klenow fragment (Thermo Scientific), third channel—400 nM of RecA and 1 mM of ATP (Sigma), fourth channel—400 nM of RecA, 1 mM of ATP and 10 μM of peptide. ..

    Labeling:

    Article Title: Blocking the RecA activity and SOS-response in bacteria with a short α-helical peptide
    Article Snippet: .. First channel contained 50 fM of streptavidin-coated polystyrene beads (2.1 μm, Spherotech), second channel—5 pM of Lambda DNA (New England Biolabs) end labeled with biotinylated nucleotides (Biotin-16-dCTP, Jena Bioscience) using Klenow fragment (Thermo Scientific), third channel—400 nM of RecA and 1 mM of ATP (Sigma), fourth channel—400 nM of RecA, 1 mM of ATP and 10 μM of peptide. ..

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  • 93
    Jena Bioscience biotinylated nucleotides
    Biotinylated Nucleotides, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated nucleotides/product/Jena Bioscience
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    biotinylated nucleotides - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

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