fluorescein 12 dutp  (Jena Bioscience)


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  • 94
    Name:
    Fluorescein 12 dUTP
    Description:

    Catalog Number:
    NU-803-FAMX-L
    Price:
    243.6
    Category:
    Nucleotides Nucleosides
    Size:
    5 x 10 µl
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    Structured Review

    Jena Bioscience fluorescein 12 dutp
    Genomic in situ hybridization combined with the (TTAGG) n telomeric probe in pachytene chromosomes in male (a-c) and female (d-f) Choristoneura fumiferana . Female-derived genomic probe was labeled with <t>fluorescein-12-dUTP</t> (green), and the telomeric probe with Cy3-dUTP (red); chromosomes were counterstained with DAPI (blue). Panels (a-c) show a male pachytene complement; arrows indicate heterochromatic block highlighted with the female genomic probe. Panels (d-f) show a female pachytene complement; “WZ” label identifies the sex chromosome bivalent (see schematic drawing in the lower left corner of panel d), where discrimination of the W chromosome is provided by the female-derived genomic probe; “N” indicates a nucleolus associated with a heterochromatic region (showing strong hybridization signals of the female genomic probe in panel d-f) of an autosome bivalent. (a, d) Merged images of preparations hybridized with female-derived genomic probe and telomeric probe, and counterstained with DAPI; (b, e) DAPI staining pattern; (c, f) hybridization pattern obtained using female-derived genomic probe. Scale bars = 10 μm.

    https://www.bioz.com/result/fluorescein 12 dutp/product/Jena Bioscience
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescein 12 dutp - by Bioz Stars, 2021-05
    94/100 stars

    Images

    1) Product Images from "Insights into the Structure of the Spruce Budworm (Choristoneura fumiferana) Genome, as Revealed by Molecular Cytogenetic Analyses and a High-Density Linkage Map"

    Article Title: Insights into the Structure of the Spruce Budworm (Choristoneura fumiferana) Genome, as Revealed by Molecular Cytogenetic Analyses and a High-Density Linkage Map

    Journal: G3: Genes|Genomes|Genetics

    doi: 10.1534/g3.118.200263

    Genomic in situ hybridization combined with the (TTAGG) n telomeric probe in pachytene chromosomes in male (a-c) and female (d-f) Choristoneura fumiferana . Female-derived genomic probe was labeled with fluorescein-12-dUTP (green), and the telomeric probe with Cy3-dUTP (red); chromosomes were counterstained with DAPI (blue). Panels (a-c) show a male pachytene complement; arrows indicate heterochromatic block highlighted with the female genomic probe. Panels (d-f) show a female pachytene complement; “WZ” label identifies the sex chromosome bivalent (see schematic drawing in the lower left corner of panel d), where discrimination of the W chromosome is provided by the female-derived genomic probe; “N” indicates a nucleolus associated with a heterochromatic region (showing strong hybridization signals of the female genomic probe in panel d-f) of an autosome bivalent. (a, d) Merged images of preparations hybridized with female-derived genomic probe and telomeric probe, and counterstained with DAPI; (b, e) DAPI staining pattern; (c, f) hybridization pattern obtained using female-derived genomic probe. Scale bars = 10 μm.
    Figure Legend Snippet: Genomic in situ hybridization combined with the (TTAGG) n telomeric probe in pachytene chromosomes in male (a-c) and female (d-f) Choristoneura fumiferana . Female-derived genomic probe was labeled with fluorescein-12-dUTP (green), and the telomeric probe with Cy3-dUTP (red); chromosomes were counterstained with DAPI (blue). Panels (a-c) show a male pachytene complement; arrows indicate heterochromatic block highlighted with the female genomic probe. Panels (d-f) show a female pachytene complement; “WZ” label identifies the sex chromosome bivalent (see schematic drawing in the lower left corner of panel d), where discrimination of the W chromosome is provided by the female-derived genomic probe; “N” indicates a nucleolus associated with a heterochromatic region (showing strong hybridization signals of the female genomic probe in panel d-f) of an autosome bivalent. (a, d) Merged images of preparations hybridized with female-derived genomic probe and telomeric probe, and counterstained with DAPI; (b, e) DAPI staining pattern; (c, f) hybridization pattern obtained using female-derived genomic probe. Scale bars = 10 μm.

    Techniques Used: In Situ Hybridization, Derivative Assay, Labeling, Blocking Assay, Hybridization, Staining

    Related Articles

    Labeling:

    Article Title: The Role of Satellite DNAs in Genome Architecture and Sex Chromosome Evolution in Crambidae Moths
    Article Snippet: .. Female and male gDNAs extracted by the CTAB procedure were labeled by nick translation with Cy3-dUTP for female gDNA or fluorescein-12-dUTP (both Jena Bioscience, Jena, Germany) for male gDNA, or vice versa. .. The nick translation mixture was composed of 500 ng of gDNA, 25 μM of each dATP, dCTP, and dGTP, 9 μM of dTTP, 16 μM of labeled nucleotides, nick translation buffer (50 mM Tris–HCl, pH 7.5, 5 mM MgCl2, 0.005% BSA), 10 mM β-mercaptoethanol, 20 U DNA Polymerase I (ThermoFisher Scientific, Waltham, MA, United States), and 0.005 U DNase I (ThermoFisher Scientific).

    Article Title: Supernumerary B Chromosomes and Plant Genome Changes: A Snapshot of Wild Populations of Aegilops speltoides Tausch (Poaceae, Triticeae)
    Article Snippet: The tandem repeats Spelt1 [ ], Spelt52 [ ], and pSc119.2 [ ], pTa71 (for localization of 45S rDNA) [ ], As5SDNAE (for localization of 5S rDNA) [ ], CCS1 (cereal centromere-specific sequence 1) [ ], and a PCR-generated synthetic probe consisting of a tandem repeat array of the short sequence TTTAGGG for the localization of telomeres [ ] were used as DNA probes for FISH. .. The DNA probes were directly labeled with Cy-3 and fluorescein-12-dUTP (Jena Bioscience, Jena, Thuringia, Germany) using a standard protocol. .. The chromosomes were differentially stained with 4′,6-diamidino-2-phenylindole (DAPI).

    Article Title: Intraspecific and intraorganismal copy number dynamics of retrotransposons and tandem repeat in Aegilops speltoides Tausch (Poaceae, Triticeae).
    Article Snippet: Transposable elements (TE) and tandem repeats (TR) compose the largest fraction of the plant genome. .. Transposable elements (TE) and tandem repeats (TR) compose the largest fraction of the plant genome. .. Transposable elements (TE) and tandem repeats (TR) compose the largest fraction of the plant genome.

    Article Title: Genotype- and Cell-Specific Dynamics of Tandem Repeat Patterns in Aegilops speltoides Tausch (Poaceae, Triticeae).
    Article Snippet: In wild plant populations, chromosome rearrangements lead to the wide intraspecific polymorphisms in the abundance and patterns of highly repetitive DNA. .. In wild plant populations, chromosome rearrangements lead to the wide intraspecific polymorphisms in the abundance and patterns of highly repetitive DNA. .. In wild plant populations, chromosome rearrangements lead to the wide intraspecific polymorphisms in the abundance and patterns of highly repetitive DNA.

    Article Title: Insights into the Structure of the Spruce Budworm (Choristoneura fumiferana) Genome, as Revealed by Molecular Cytogenetic Analyses and a High-Density Linkage Map
    Article Snippet: For GISH, total gDNA from males and females was isolated using the CTAB method adapted from . .. Female gDNA was labeled with fluorescein-12-dUTP (Jena Bioscience) using the Nick Translation Kit (Abbott Molecular Inc.), with 4 h of incubation at 15°, and male gDNA was sonicated using a Sonopuls HD 2070 (Bandelin Electric, Berlin, Germany) and used as competitor DNA. .. For GISH combined with tel-FISH, the probe mixture contained fluorescein-labeled female gDNA (300 ng), Cy3-labeled telomeric probe (100 ng), sonicated male gDNA (3 μg) and sonicated salmon sperm DNA (25 μg).

    Nick Translation:

    Article Title: The Role of Satellite DNAs in Genome Architecture and Sex Chromosome Evolution in Crambidae Moths
    Article Snippet: .. Female and male gDNAs extracted by the CTAB procedure were labeled by nick translation with Cy3-dUTP for female gDNA or fluorescein-12-dUTP (both Jena Bioscience, Jena, Germany) for male gDNA, or vice versa. .. The nick translation mixture was composed of 500 ng of gDNA, 25 μM of each dATP, dCTP, and dGTP, 9 μM of dTTP, 16 μM of labeled nucleotides, nick translation buffer (50 mM Tris–HCl, pH 7.5, 5 mM MgCl2, 0.005% BSA), 10 mM β-mercaptoethanol, 20 U DNA Polymerase I (ThermoFisher Scientific, Waltham, MA, United States), and 0.005 U DNase I (ThermoFisher Scientific).

    Article Title: Insights into the Structure of the Spruce Budworm (Choristoneura fumiferana) Genome, as Revealed by Molecular Cytogenetic Analyses and a High-Density Linkage Map
    Article Snippet: For GISH, total gDNA from males and females was isolated using the CTAB method adapted from . .. Female gDNA was labeled with fluorescein-12-dUTP (Jena Bioscience) using the Nick Translation Kit (Abbott Molecular Inc.), with 4 h of incubation at 15°, and male gDNA was sonicated using a Sonopuls HD 2070 (Bandelin Electric, Berlin, Germany) and used as competitor DNA. .. For GISH combined with tel-FISH, the probe mixture contained fluorescein-labeled female gDNA (300 ng), Cy3-labeled telomeric probe (100 ng), sonicated male gDNA (3 μg) and sonicated salmon sperm DNA (25 μg).

    other:

    Article Title: Repetitive DNA in the Architecture, Repatterning, and Diversification of the Genome of Aegilops speltoides Tausch (Poaceae, Triticeae)
    Article Snippet: Lasers used: EX 405 EM 430–470 for DAPI, EX 488 EM 500–540 for Fluorescein-12-dUTP, and EX 552 EM 560–590 for Cy3.

    Oligonucleotide Labeling:

    Article Title: Intraspecific and intraorganismal copy number dynamics of retrotransposons and tandem repeat in Aegilops speltoides Tausch (Poaceae, Triticeae).
    Article Snippet: Transposable elements (TE) and tandem repeats (TR) compose the largest fraction of the plant genome. .. Transposable elements (TE) and tandem repeats (TR) compose the largest fraction of the plant genome. .. Transposable elements (TE) and tandem repeats (TR) compose the largest fraction of the plant genome.

    Imaging:

    Article Title: Repetitive DNA in the Architecture, Repatterning, and Diversification of the Genome of Aegilops speltoides Tausch (Poaceae, Triticeae)
    Article Snippet: AT-specific 4′,6-diamidino-2-phenylindole (DAPI) fluorochrome was used for differential staining. .. Epi-Fluorescence Imaging The slides were examined on a Leica DMR fluorescent microscope equipped with a DFC300 FX CCD color camera using following filter sets: A for DAPI, I3 for Fluorescein-12-dUTP, N2.1 for Cy3, FI/RH for Fluorescein-12-dUTP/Cy3, and B/G/R for blue/green/red fluorescence. .. Confocal Image Acquisition and Analysis Confocal imaging was done using a LEICA SP8 (CTR6000) microscope with a Leica HC PL APO CS2 × 63 N.A 1.4 oil objective.

    Microscopy:

    Article Title: Repetitive DNA in the Architecture, Repatterning, and Diversification of the Genome of Aegilops speltoides Tausch (Poaceae, Triticeae)
    Article Snippet: AT-specific 4′,6-diamidino-2-phenylindole (DAPI) fluorochrome was used for differential staining. .. Epi-Fluorescence Imaging The slides were examined on a Leica DMR fluorescent microscope equipped with a DFC300 FX CCD color camera using following filter sets: A for DAPI, I3 for Fluorescein-12-dUTP, N2.1 for Cy3, FI/RH for Fluorescein-12-dUTP/Cy3, and B/G/R for blue/green/red fluorescence. .. Confocal Image Acquisition and Analysis Confocal imaging was done using a LEICA SP8 (CTR6000) microscope with a Leica HC PL APO CS2 × 63 N.A 1.4 oil objective.

    Fluorescence:

    Article Title: Repetitive DNA in the Architecture, Repatterning, and Diversification of the Genome of Aegilops speltoides Tausch (Poaceae, Triticeae)
    Article Snippet: AT-specific 4′,6-diamidino-2-phenylindole (DAPI) fluorochrome was used for differential staining. .. Epi-Fluorescence Imaging The slides were examined on a Leica DMR fluorescent microscope equipped with a DFC300 FX CCD color camera using following filter sets: A for DAPI, I3 for Fluorescein-12-dUTP, N2.1 for Cy3, FI/RH for Fluorescein-12-dUTP/Cy3, and B/G/R for blue/green/red fluorescence. .. Confocal Image Acquisition and Analysis Confocal imaging was done using a LEICA SP8 (CTR6000) microscope with a Leica HC PL APO CS2 × 63 N.A 1.4 oil objective.

    Incubation:

    Article Title: Insights into the Structure of the Spruce Budworm (Choristoneura fumiferana) Genome, as Revealed by Molecular Cytogenetic Analyses and a High-Density Linkage Map
    Article Snippet: For GISH, total gDNA from males and females was isolated using the CTAB method adapted from . .. Female gDNA was labeled with fluorescein-12-dUTP (Jena Bioscience) using the Nick Translation Kit (Abbott Molecular Inc.), with 4 h of incubation at 15°, and male gDNA was sonicated using a Sonopuls HD 2070 (Bandelin Electric, Berlin, Germany) and used as competitor DNA. .. For GISH combined with tel-FISH, the probe mixture contained fluorescein-labeled female gDNA (300 ng), Cy3-labeled telomeric probe (100 ng), sonicated male gDNA (3 μg) and sonicated salmon sperm DNA (25 μg).

    Sonication:

    Article Title: Insights into the Structure of the Spruce Budworm (Choristoneura fumiferana) Genome, as Revealed by Molecular Cytogenetic Analyses and a High-Density Linkage Map
    Article Snippet: For GISH, total gDNA from males and females was isolated using the CTAB method adapted from . .. Female gDNA was labeled with fluorescein-12-dUTP (Jena Bioscience) using the Nick Translation Kit (Abbott Molecular Inc.), with 4 h of incubation at 15°, and male gDNA was sonicated using a Sonopuls HD 2070 (Bandelin Electric, Berlin, Germany) and used as competitor DNA. .. For GISH combined with tel-FISH, the probe mixture contained fluorescein-labeled female gDNA (300 ng), Cy3-labeled telomeric probe (100 ng), sonicated male gDNA (3 μg) and sonicated salmon sperm DNA (25 μg).

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  • 94
    Jena Bioscience fluorescein 12 dutp
    Genomic in situ hybridization combined with the (TTAGG) n telomeric probe in pachytene chromosomes in male (a-c) and female (d-f) Choristoneura fumiferana . Female-derived genomic probe was labeled with <t>fluorescein-12-dUTP</t> (green), and the telomeric probe with Cy3-dUTP (red); chromosomes were counterstained with DAPI (blue). Panels (a-c) show a male pachytene complement; arrows indicate heterochromatic block highlighted with the female genomic probe. Panels (d-f) show a female pachytene complement; “WZ” label identifies the sex chromosome bivalent (see schematic drawing in the lower left corner of panel d), where discrimination of the W chromosome is provided by the female-derived genomic probe; “N” indicates a nucleolus associated with a heterochromatic region (showing strong hybridization signals of the female genomic probe in panel d-f) of an autosome bivalent. (a, d) Merged images of preparations hybridized with female-derived genomic probe and telomeric probe, and counterstained with DAPI; (b, e) DAPI staining pattern; (c, f) hybridization pattern obtained using female-derived genomic probe. Scale bars = 10 μm.
    Fluorescein 12 Dutp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein 12 dutp/product/Jena Bioscience
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescein 12 dutp - by Bioz Stars, 2021-05
    94/100 stars
      Buy from Supplier

    Image Search Results


    Genomic in situ hybridization combined with the (TTAGG) n telomeric probe in pachytene chromosomes in male (a-c) and female (d-f) Choristoneura fumiferana . Female-derived genomic probe was labeled with fluorescein-12-dUTP (green), and the telomeric probe with Cy3-dUTP (red); chromosomes were counterstained with DAPI (blue). Panels (a-c) show a male pachytene complement; arrows indicate heterochromatic block highlighted with the female genomic probe. Panels (d-f) show a female pachytene complement; “WZ” label identifies the sex chromosome bivalent (see schematic drawing in the lower left corner of panel d), where discrimination of the W chromosome is provided by the female-derived genomic probe; “N” indicates a nucleolus associated with a heterochromatic region (showing strong hybridization signals of the female genomic probe in panel d-f) of an autosome bivalent. (a, d) Merged images of preparations hybridized with female-derived genomic probe and telomeric probe, and counterstained with DAPI; (b, e) DAPI staining pattern; (c, f) hybridization pattern obtained using female-derived genomic probe. Scale bars = 10 μm.

    Journal: G3: Genes|Genomes|Genetics

    Article Title: Insights into the Structure of the Spruce Budworm (Choristoneura fumiferana) Genome, as Revealed by Molecular Cytogenetic Analyses and a High-Density Linkage Map

    doi: 10.1534/g3.118.200263

    Figure Lengend Snippet: Genomic in situ hybridization combined with the (TTAGG) n telomeric probe in pachytene chromosomes in male (a-c) and female (d-f) Choristoneura fumiferana . Female-derived genomic probe was labeled with fluorescein-12-dUTP (green), and the telomeric probe with Cy3-dUTP (red); chromosomes were counterstained with DAPI (blue). Panels (a-c) show a male pachytene complement; arrows indicate heterochromatic block highlighted with the female genomic probe. Panels (d-f) show a female pachytene complement; “WZ” label identifies the sex chromosome bivalent (see schematic drawing in the lower left corner of panel d), where discrimination of the W chromosome is provided by the female-derived genomic probe; “N” indicates a nucleolus associated with a heterochromatic region (showing strong hybridization signals of the female genomic probe in panel d-f) of an autosome bivalent. (a, d) Merged images of preparations hybridized with female-derived genomic probe and telomeric probe, and counterstained with DAPI; (b, e) DAPI staining pattern; (c, f) hybridization pattern obtained using female-derived genomic probe. Scale bars = 10 μm.

    Article Snippet: Female gDNA was labeled with fluorescein-12-dUTP (Jena Bioscience) using the Nick Translation Kit (Abbott Molecular Inc.), with 4 h of incubation at 15°, and male gDNA was sonicated using a Sonopuls HD 2070 (Bandelin Electric, Berlin, Germany) and used as competitor DNA.

    Techniques: In Situ Hybridization, Derivative Assay, Labeling, Blocking Assay, Hybridization, Staining