cy5 xx dutp  (Jena Bioscience)


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  • 92
    Name:
    Aminoallyl dUTP XX Cy5
    Description:

    Catalog Number:
    NU-803-XX-CY5-L
    Price:
    349.4
    Category:
    Nucleotides Nucleosides
    Size:
    5 x 10 µl
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    Structured Review

    Jena Bioscience cy5 xx dutp

    https://www.bioz.com/result/cy5 xx dutp/product/Jena Bioscience
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cy5 xx dutp - by Bioz Stars, 2021-06
    92/100 stars

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    Related Articles

    Fluorescence In Situ Hybridization:

    Article Title: Functional linkage of gene fusions to cancer cell fitness assessed by pharmacological and CRISPR-Cas9 screening
    Article Snippet: Probes were generated by whole-genome amplification with GenomePlex Whole Genome Amplification Kits (Sigma-Aldrich), from purified BAC fosmid DNA as described previously . .. For interphase and metaphase FISH, probes were labeled directly with Atto488-XX-dUTP, Cy3-XX-dUTP, Texas Red-12-dUTP, and Cy5-XX-dUTP (Jena Bioscience), respectively. .. Slides pre-treatment included a 10 min fixation in acetone (Sigma-Aldrich), followed by baking at 65 °C for 1 h. Metaphase spreads on slides were denatured by immersion in an alkaline denaturation solution (0.5 M NaOH, 1.0 M NaCl) for 7–10 min, followed by rinsing in 1M Tris-HCl (pH 7.4) solution for 3 min, 1× PBS for 3 min and dehydration through a 70, 90, and 100% ethanol series.

    Labeling:

    Article Title: Functional linkage of gene fusions to cancer cell fitness assessed by pharmacological and CRISPR-Cas9 screening
    Article Snippet: Probes were generated by whole-genome amplification with GenomePlex Whole Genome Amplification Kits (Sigma-Aldrich), from purified BAC fosmid DNA as described previously . .. For interphase and metaphase FISH, probes were labeled directly with Atto488-XX-dUTP, Cy3-XX-dUTP, Texas Red-12-dUTP, and Cy5-XX-dUTP (Jena Bioscience), respectively. .. Slides pre-treatment included a 10 min fixation in acetone (Sigma-Aldrich), followed by baking at 65 °C for 1 h. Metaphase spreads on slides were denatured by immersion in an alkaline denaturation solution (0.5 M NaOH, 1.0 M NaCl) for 7–10 min, followed by rinsing in 1M Tris-HCl (pH 7.4) solution for 3 min, 1× PBS for 3 min and dehydration through a 70, 90, and 100% ethanol series.

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  • 99
    Jena Bioscience cy5 modified
    Direct visualization of individual plectonemes on supercoiled DNA. ( a ) Schematic of the ISD assay. (top) A flow-stretched DNA is doubly-tethered on a PEG-coated surface via streptavidin-biotin linkage. One-end of the DNA is labeled with <t>Cy5-fluorophores</t> (red stars) for identifying the direction of each DNA molecule. (bottom) Binding of SxO fluorophores induces supercoiling to the torsionally constrained DNA molecule. ( b ) Representative fluorescence images of a supercoiled DNA molecule. Left: Snap-shot image of a supercoiled DNA with 100 ms exposure. Yellow arrows highlight higher DNA density, that is individual plectonemes. Right: Time-averaged DNA image by stacking 1000 images (of 100 ms exposure each). Arrows indicate peaks in the inhomogeneous average density of plectonemes. ( c ) AT-contents of two DNA samples: template1 and template2 binned to 300 bp. ( d ) Plectoneme densities obtained from individual DNA molecules. (top) Plectoneme density on template1 (grey thin lines, n = 70) and their ensemble average (red line). Arrow indicates a strong plectoneme pinning site. (bottom) Plectoneme densities obtained from individual DNA molecules of template2 (grey thin lines, n = 120) and their ensemble average (black line).
    Cy5 Modified, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy5 modified/product/Jena Bioscience
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cy5 modified - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    92
    Jena Bioscience cy5 xx dutp
    Direct visualization of individual plectonemes on supercoiled DNA. ( a ) Schematic of the ISD assay. (top) A flow-stretched DNA is doubly-tethered on a PEG-coated surface via streptavidin-biotin linkage. One-end of the DNA is labeled with <t>Cy5-fluorophores</t> (red stars) for identifying the direction of each DNA molecule. (bottom) Binding of SxO fluorophores induces supercoiling to the torsionally constrained DNA molecule. ( b ) Representative fluorescence images of a supercoiled DNA molecule. Left: Snap-shot image of a supercoiled DNA with 100 ms exposure. Yellow arrows highlight higher DNA density, that is individual plectonemes. Right: Time-averaged DNA image by stacking 1000 images (of 100 ms exposure each). Arrows indicate peaks in the inhomogeneous average density of plectonemes. ( c ) AT-contents of two DNA samples: template1 and template2 binned to 300 bp. ( d ) Plectoneme densities obtained from individual DNA molecules. (top) Plectoneme density on template1 (grey thin lines, n = 70) and their ensemble average (red line). Arrow indicates a strong plectoneme pinning site. (bottom) Plectoneme densities obtained from individual DNA molecules of template2 (grey thin lines, n = 120) and their ensemble average (black line).
    Cy5 Xx Dutp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy5 xx dutp/product/Jena Bioscience
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cy5 xx dutp - by Bioz Stars, 2021-06
    92/100 stars
      Buy from Supplier

    Image Search Results


    Direct visualization of individual plectonemes on supercoiled DNA. ( a ) Schematic of the ISD assay. (top) A flow-stretched DNA is doubly-tethered on a PEG-coated surface via streptavidin-biotin linkage. One-end of the DNA is labeled with Cy5-fluorophores (red stars) for identifying the direction of each DNA molecule. (bottom) Binding of SxO fluorophores induces supercoiling to the torsionally constrained DNA molecule. ( b ) Representative fluorescence images of a supercoiled DNA molecule. Left: Snap-shot image of a supercoiled DNA with 100 ms exposure. Yellow arrows highlight higher DNA density, that is individual plectonemes. Right: Time-averaged DNA image by stacking 1000 images (of 100 ms exposure each). Arrows indicate peaks in the inhomogeneous average density of plectonemes. ( c ) AT-contents of two DNA samples: template1 and template2 binned to 300 bp. ( d ) Plectoneme densities obtained from individual DNA molecules. (top) Plectoneme density on template1 (grey thin lines, n = 70) and their ensemble average (red line). Arrow indicates a strong plectoneme pinning site. (bottom) Plectoneme densities obtained from individual DNA molecules of template2 (grey thin lines, n = 120) and their ensemble average (black line).

    Journal: eLife

    Article Title: DNA sequence encodes the position of DNA supercoils

    doi: 10.7554/eLife.36557

    Figure Lengend Snippet: Direct visualization of individual plectonemes on supercoiled DNA. ( a ) Schematic of the ISD assay. (top) A flow-stretched DNA is doubly-tethered on a PEG-coated surface via streptavidin-biotin linkage. One-end of the DNA is labeled with Cy5-fluorophores (red stars) for identifying the direction of each DNA molecule. (bottom) Binding of SxO fluorophores induces supercoiling to the torsionally constrained DNA molecule. ( b ) Representative fluorescence images of a supercoiled DNA molecule. Left: Snap-shot image of a supercoiled DNA with 100 ms exposure. Yellow arrows highlight higher DNA density, that is individual plectonemes. Right: Time-averaged DNA image by stacking 1000 images (of 100 ms exposure each). Arrows indicate peaks in the inhomogeneous average density of plectonemes. ( c ) AT-contents of two DNA samples: template1 and template2 binned to 300 bp. ( d ) Plectoneme densities obtained from individual DNA molecules. (top) Plectoneme density on template1 (grey thin lines, n = 70) and their ensemble average (red line). Arrow indicates a strong plectoneme pinning site. (bottom) Plectoneme densities obtained from individual DNA molecules of template2 (grey thin lines, n = 120) and their ensemble average (black line).

    Article Snippet: The ‘Cy5-biotin handle’ and ‘biotin handle’ were prepared by PCR methods in the presence of Cy5-modified and/or biotinylated dUTP (aminoallyl-dUTP-Cy5 and biotin-16-dUTP, Jena Bioscience).

    Techniques: Flow Cytometry, Labeling, Binding Assay, Fluorescence, Mass Spectrometry