fluorescein 12 dutp  (Jena Bioscience)


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    Name:
    Fluorescein 12 dUTP
    Description:
    Fluorescein 12 dUTP is recommended for direct enzymatic labeling of DNA cDNA e g by PCR and Nick Translation It is incorporated as substitute for its natural counterpart dTTP The resulting Dye labeled DNA cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray based gene expression profiling Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine Recommended Fluorescein 12 dUTP dTTP ratio for PCR and Nick Translation 30 50 Fluorescein 12 dUTP 70 50 dTTP Please note Protect the Dye labeled dUTP from exposure to light and carry out experimental procedures in low light conditions The optimal final concentration of the Dye labeled dUTP may very depending on the application and assay conditions For optimal product yields and high incorporation rates an individual optimization of the Dye labeled dUTP dTTP ratio is recommended
    Catalog Number:
    nu-803-famx-l
    Price:
    243.6
    Applications:
    Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data, [1] - Nick Translation with DNAse I/ DNA Polymerase I in-house data
    Purity:
    ≥ 95 % (HPLC)
    Category:
    Nucleotides Nucleosides
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    Structured Review

    Jena Bioscience fluorescein 12 dutp
    Fluorescein 12 dUTP
    Fluorescein 12 dUTP is recommended for direct enzymatic labeling of DNA cDNA e g by PCR and Nick Translation It is incorporated as substitute for its natural counterpart dTTP The resulting Dye labeled DNA cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray based gene expression profiling Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine Recommended Fluorescein 12 dUTP dTTP ratio for PCR and Nick Translation 30 50 Fluorescein 12 dUTP 70 50 dTTP Please note Protect the Dye labeled dUTP from exposure to light and carry out experimental procedures in low light conditions The optimal final concentration of the Dye labeled dUTP may very depending on the application and assay conditions For optimal product yields and high incorporation rates an individual optimization of the Dye labeled dUTP dTTP ratio is recommended
    https://www.bioz.com/result/fluorescein 12 dutp/product/Jena Bioscience
    Average 93 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    fluorescein 12 dutp - by Bioz Stars, 2020-08
    93/100 stars

    Images

    1) Product Images from "Insights into the Structure of the Spruce Budworm (Choristoneura fumiferana) Genome, as Revealed by Molecular Cytogenetic Analyses and a High-Density Linkage Map"

    Article Title: Insights into the Structure of the Spruce Budworm (Choristoneura fumiferana) Genome, as Revealed by Molecular Cytogenetic Analyses and a High-Density Linkage Map

    Journal: G3: Genes|Genomes|Genetics

    doi: 10.1534/g3.118.200263

    Genomic in situ hybridization combined with the (TTAGG) n telomeric probe in pachytene chromosomes in male (a-c) and female (d-f) Choristoneura fumiferana . Female-derived genomic probe was labeled with fluorescein-12-dUTP (green), and the telomeric probe with Cy3-dUTP (red); chromosomes were counterstained with DAPI (blue). Panels (a-c) show a male pachytene complement; arrows indicate heterochromatic block highlighted with the female genomic probe. Panels (d-f) show a female pachytene complement; “WZ” label identifies the sex chromosome bivalent (see schematic drawing in the lower left corner of panel d), where discrimination of the W chromosome is provided by the female-derived genomic probe; “N” indicates a nucleolus associated with a heterochromatic region (showing strong hybridization signals of the female genomic probe in panel d-f) of an autosome bivalent. (a, d) Merged images of preparations hybridized with female-derived genomic probe and telomeric probe, and counterstained with DAPI; (b, e) DAPI staining pattern; (c, f) hybridization pattern obtained using female-derived genomic probe. Scale bars = 10 μm.
    Figure Legend Snippet: Genomic in situ hybridization combined with the (TTAGG) n telomeric probe in pachytene chromosomes in male (a-c) and female (d-f) Choristoneura fumiferana . Female-derived genomic probe was labeled with fluorescein-12-dUTP (green), and the telomeric probe with Cy3-dUTP (red); chromosomes were counterstained with DAPI (blue). Panels (a-c) show a male pachytene complement; arrows indicate heterochromatic block highlighted with the female genomic probe. Panels (d-f) show a female pachytene complement; “WZ” label identifies the sex chromosome bivalent (see schematic drawing in the lower left corner of panel d), where discrimination of the W chromosome is provided by the female-derived genomic probe; “N” indicates a nucleolus associated with a heterochromatic region (showing strong hybridization signals of the female genomic probe in panel d-f) of an autosome bivalent. (a, d) Merged images of preparations hybridized with female-derived genomic probe and telomeric probe, and counterstained with DAPI; (b, e) DAPI staining pattern; (c, f) hybridization pattern obtained using female-derived genomic probe. Scale bars = 10 μm.

    Techniques Used: In Situ Hybridization, Derivative Assay, Labeling, Blocking Assay, Hybridization, Staining

    Related Articles

    Fluorescence:

    Article Title: Repetitive DNA in the Architecture, Repatterning, and Diversification of the Genome of Aegilops speltoides Tausch (Poaceae, Triticeae)
    Article Snippet: .. The slides were examined on a Leica DMR fluorescent microscope equipped with a DFC300 FX CCD color camera using following filter sets: A for DAPI, I3 for Fluorescein-12-dUTP, N2.1 for Cy3, FI/RH for Fluorescein-12-dUTP/Cy3, and B/G/R for blue/green/red fluorescence. .. Confocal imaging was done using a LEICA SP8 (CTR6000) microscope with a Leica HC PL APO CS2 × 63 N.A 1.4 oil objective.

    Nick Translation:

    Article Title: Insights into the Structure of the Spruce Budworm (Choristoneura fumiferana) Genome, as Revealed by Molecular Cytogenetic Analyses and a High-Density Linkage Map
    Article Snippet: .. Female gDNA was labeled with fluorescein-12-dUTP (Jena Bioscience) using the Nick Translation Kit (Abbott Molecular Inc.), with 4 h of incubation at 15°, and male gDNA was sonicated using a Sonopuls HD 2070 (Bandelin Electric, Berlin, Germany) and used as competitor DNA. .. For GISH combined with tel-FISH, the probe mixture contained fluorescein-labeled female gDNA (300 ng), Cy3-labeled telomeric probe (100 ng), sonicated male gDNA (3 μg) and sonicated salmon sperm DNA (25 μg).

    Article Title: Neo-sex Chromosomes in the Monarch Butterfly, Danaus plexippus
    Article Snippet: .. Hybridization probes were prepared from the entire complement of extracted male or female gDNA, which was labeled by aminoallyl-dUTP-Cy3 or fluorescein-12-dUTP (both Jena Bioscience GmbH, Jena, Germany) using the Nick Translation Kit (Abbott Molecular Inc., Des Plaines, IL) with 7 hr incubation at 15° ( ). ..

    Article Title: Sex Chromosomes of the Iconic Moth Abraxas grossulariata (Lepidoptera, Geometridae) and Its Congener A. sylvata
    Article Snippet: .. The 25 μL nick translation reaction contained 500 ng gDNA; 25 µM dATP, dCTP, and dGTP; 9 µM dTTP; 16 µM labeled nucleotides with either Cy3-dUTP (male gDNA) or fluorescein-12-dUTP (female gDNA) (both Jena Bioscience, Jena, Germany); 1× nick translation buffer and 5 µL of nick translation enzyme mix. .. The reaction was incubated at 15 °C for 6–7 h. CGH was performed according to a published protocol [ ] with modifications described previously [ ].

    Labeling:

    Article Title: Insights into the Structure of the Spruce Budworm (Choristoneura fumiferana) Genome, as Revealed by Molecular Cytogenetic Analyses and a High-Density Linkage Map
    Article Snippet: .. Female gDNA was labeled with fluorescein-12-dUTP (Jena Bioscience) using the Nick Translation Kit (Abbott Molecular Inc.), with 4 h of incubation at 15°, and male gDNA was sonicated using a Sonopuls HD 2070 (Bandelin Electric, Berlin, Germany) and used as competitor DNA. .. For GISH combined with tel-FISH, the probe mixture contained fluorescein-labeled female gDNA (300 ng), Cy3-labeled telomeric probe (100 ng), sonicated male gDNA (3 μg) and sonicated salmon sperm DNA (25 μg).

    Article Title: Repetitive DNA in the Architecture, Repatterning, and Diversification of the Genome of Aegilops speltoides Tausch (Poaceae, Triticeae)
    Article Snippet: .. The DNA probes were directly labeled with Cy-3, Fluorescein-12-dUTP, and ATTO-425 (Jena Bioscience, Germany). .. AT-specific 4′,6-diamidino-2-phenylindole (DAPI) fluorochrome was used for differential staining.

    Article Title: Transposable Elements in the Organization and Diversification of the Genome of Aegilops speltoides Tausch (Poaceae, Triticeae)
    Article Snippet: .. The DNA probes were directly labeled with Cy-3, fluorescein-12-dUTP, and ATTO-425 (Jena Bioscience, Germany). .. AT-specific 4′,6-diamidino-2-phenylindole (DAPI) and GC-specific chromomycin A3 (CMA3 ) fluorochromes were used for differential staining to reveal AT-enriched heterochromatin patterns and GC-enriched heterochromatic clusters in the nuclear organizer regions (NORs) on chromosomes 1 and 6 in the Ae. speltoides genome.

    Article Title: Neo-sex Chromosomes in the Monarch Butterfly, Danaus plexippus
    Article Snippet: .. Hybridization probes were prepared from the entire complement of extracted male or female gDNA, which was labeled by aminoallyl-dUTP-Cy3 or fluorescein-12-dUTP (both Jena Bioscience GmbH, Jena, Germany) using the Nick Translation Kit (Abbott Molecular Inc., Des Plaines, IL) with 7 hr incubation at 15° ( ). ..

    Article Title: Sex Chromosomes of the Iconic Moth Abraxas grossulariata (Lepidoptera, Geometridae) and Its Congener A. sylvata
    Article Snippet: .. The 25 μL nick translation reaction contained 500 ng gDNA; 25 µM dATP, dCTP, and dGTP; 9 µM dTTP; 16 µM labeled nucleotides with either Cy3-dUTP (male gDNA) or fluorescein-12-dUTP (female gDNA) (both Jena Bioscience, Jena, Germany); 1× nick translation buffer and 5 µL of nick translation enzyme mix. .. The reaction was incubated at 15 °C for 6–7 h. CGH was performed according to a published protocol [ ] with modifications described previously [ ].

    Incubation:

    Article Title: Insights into the Structure of the Spruce Budworm (Choristoneura fumiferana) Genome, as Revealed by Molecular Cytogenetic Analyses and a High-Density Linkage Map
    Article Snippet: .. Female gDNA was labeled with fluorescein-12-dUTP (Jena Bioscience) using the Nick Translation Kit (Abbott Molecular Inc.), with 4 h of incubation at 15°, and male gDNA was sonicated using a Sonopuls HD 2070 (Bandelin Electric, Berlin, Germany) and used as competitor DNA. .. For GISH combined with tel-FISH, the probe mixture contained fluorescein-labeled female gDNA (300 ng), Cy3-labeled telomeric probe (100 ng), sonicated male gDNA (3 μg) and sonicated salmon sperm DNA (25 μg).

    Article Title: Neo-sex Chromosomes in the Monarch Butterfly, Danaus plexippus
    Article Snippet: .. Hybridization probes were prepared from the entire complement of extracted male or female gDNA, which was labeled by aminoallyl-dUTP-Cy3 or fluorescein-12-dUTP (both Jena Bioscience GmbH, Jena, Germany) using the Nick Translation Kit (Abbott Molecular Inc., Des Plaines, IL) with 7 hr incubation at 15° ( ). ..

    Microscopy:

    Article Title: Repetitive DNA in the Architecture, Repatterning, and Diversification of the Genome of Aegilops speltoides Tausch (Poaceae, Triticeae)
    Article Snippet: .. The slides were examined on a Leica DMR fluorescent microscope equipped with a DFC300 FX CCD color camera using following filter sets: A for DAPI, I3 for Fluorescein-12-dUTP, N2.1 for Cy3, FI/RH for Fluorescein-12-dUTP/Cy3, and B/G/R for blue/green/red fluorescence. .. Confocal imaging was done using a LEICA SP8 (CTR6000) microscope with a Leica HC PL APO CS2 × 63 N.A 1.4 oil objective.

    Sonication:

    Article Title: Insights into the Structure of the Spruce Budworm (Choristoneura fumiferana) Genome, as Revealed by Molecular Cytogenetic Analyses and a High-Density Linkage Map
    Article Snippet: .. Female gDNA was labeled with fluorescein-12-dUTP (Jena Bioscience) using the Nick Translation Kit (Abbott Molecular Inc.), with 4 h of incubation at 15°, and male gDNA was sonicated using a Sonopuls HD 2070 (Bandelin Electric, Berlin, Germany) and used as competitor DNA. .. For GISH combined with tel-FISH, the probe mixture contained fluorescein-labeled female gDNA (300 ng), Cy3-labeled telomeric probe (100 ng), sonicated male gDNA (3 μg) and sonicated salmon sperm DNA (25 μg).

    Hybridization:

    Article Title: Neo-sex Chromosomes in the Monarch Butterfly, Danaus plexippus
    Article Snippet: .. Hybridization probes were prepared from the entire complement of extracted male or female gDNA, which was labeled by aminoallyl-dUTP-Cy3 or fluorescein-12-dUTP (both Jena Bioscience GmbH, Jena, Germany) using the Nick Translation Kit (Abbott Molecular Inc., Des Plaines, IL) with 7 hr incubation at 15° ( ). ..

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    Jena Bioscience aminoallyl dutp cy3 jena bioscience
    Aminoallyl Dutp Cy3 Jena Bioscience, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aminoallyl dutp cy3 jena bioscience/product/Jena Bioscience
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    aminoallyl dutp cy3 jena bioscience - by Bioz Stars, 2020-08
    93/100 stars
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