biotin 11 dutp  (Jena Bioscience)


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    Name:
    Biotin 11 dUTP
    Description:
    Biotin 11 dUTP is enzymatically incorporated into DNA cDNA as substitute for its natural counterpart dTTP The resulting Biotin labeled DNA cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase HRP alkaline phosphatase AP a fluorescent dye or agarose magnetic beads Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11 atom linker attached to the C5 position of uridine Recommended Biotin 11 dUTP dTTP ratio for PCR and Nick Translation 50 Biotin 11 dUTP 50 dTTP Please note The optimal final concentration of Biotin 11 dUTP may very depending on the application and assay conditions For optimal product yields and high incorporation rates an individual optimization of the Biotin 11 dUTP dTTP ratio is recommended
    Catalog Number:
    nu-803-biox-l
    Price:
    234.09
    Applications:
    Incorporation into DNA/cDNA by - PCR with Taq polymerase [1,2] & in-house data - Nick Translation with DNAse I/ DNA Polymerase I [3,4] & in-house data - Primer Extension with Klenow 3'-5' exo- [5] - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)[6] - Reverse Transcription with MMLV Reverse Transcriptase [7]
    Purity:
    ≥ 95 % (HPLC)
    Category:
    Nucleotides Nucleosides
    Buy from Supplier


    Structured Review

    Jena Bioscience biotin 11 dutp
    Biotin 11 dUTP is enzymatically incorporated into DNA cDNA as substitute for its natural counterpart dTTP The resulting Biotin labeled DNA cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase HRP alkaline phosphatase AP a fluorescent dye or agarose magnetic beads Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11 atom linker attached to the C5 position of uridine Recommended Biotin 11 dUTP dTTP ratio for PCR and Nick Translation 50 Biotin 11 dUTP 50 dTTP Please note The optimal final concentration of Biotin 11 dUTP may very depending on the application and assay conditions For optimal product yields and high incorporation rates an individual optimization of the Biotin 11 dUTP dTTP ratio is recommended
    https://www.bioz.com/result/biotin 11 dutp/product/Jena Bioscience
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    biotin 11 dutp - by Bioz Stars, 2020-08
    93/100 stars

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    Related Articles

    Random Hexamer Labeling:

    Article Title: Identification of the Main Regulator Responsible for Synthesis of the Typical Yellow Pigment Produced by Trichoderma reesei
    Article Snippet: .. Labeling of the probe was performed by using a Klenow fragment (exo-) (Thermo Scientific), random hexamer primers, and biotin-11-dUTP (Jena Bioscience, Jena, Germany). .. Signals were visualized by using Poly-HRP conjugated to streptavidin and ECL Plus Western blotting substrate (both from Thermo Scientific) on a ChemiDoc MP imaging system (Bio-Rad Laboratories, Hercules, CA).

    Article Title: Xpp1 regulates the expression of xylanases, but not of cellulases in Trichoderma reesei
    Article Snippet: .. Labeling of the probe was performed using a Klenow Fragment (exo-) (Thermo Scientific), random hexamer primers, and biotin-11-dUTP (Jena Bioscience, Jena, Germany). .. Signals were visualized by using Poly-HRP conjugated to streptavidin and ECL Plus Western Blotting substrate (both Thermo Scientific Pierce, part of Life Technologies) on a ChemiDoc MP (Bio-Rad Laboratories, Hercules, USA).

    Article Title: Fusion transcription factors for strong, constitutive expression of cellulases and xylanases in Trichoderma reesei
    Article Snippet: .. Labeling of the probe was performed using a Klenow Fragment (exo-) (Thermo Scientific), random hexamer primers, and biotin-11-dUTP (Jena Bioscience, Jena, Germany). .. Signals were visualized using Poly-HRP conjugated to streptavidin and ECL Plus Western Blotting substrate (both Thermo Scientific) on a ChemiDoc MP (Bio-Rad Laboratories, Hercules, USA).

    Article Title: Influence of cis Element Arrangement on Promoter Strength in Trichoderma reesei
    Article Snippet: .. Labeling of the probe was performed by using the Klenow fragment (exo− ) (Thermo Fisher Scientific), random hexamer primers, and biotin-11-dUTP (Jena Bioscience, Jena, Germany). .. Signals were visualized by using poly-horseradish peroxidase (poly-HRP; Pierce) conjugated to streptavidin and ECL plus Western blotting substrate (Life Technologies) (both from Thermo Fisher Scientific) on a ChemiDoc MP (Bio-Rad Laboratories, Hercules, CA, USA).

    Article Title: Novel Strategies for Genomic Manipulation of Trichoderma reesei with the Purpose of Strain Engineering
    Article Snippet: .. Labeling of the probe was performed by using Klenow fragment (exo−) (Thermo Scientific), random hexamer primers, and biotin-11-dUTP (Jena Bioscience, Jena, Germany). .. Signals were visualized by using poly-horseradish peroxidase (poly-HRP) conjugated to streptavidin and ECL Plus Western blotting substrate (both from Thermo Scientific Pierce, Life Technologies) on a ChemiDoc MP (Bio-Rad Laboratories, Hercules, CA, USA).

    Labeling:

    Article Title: Identification of the Main Regulator Responsible for Synthesis of the Typical Yellow Pigment Produced by Trichoderma reesei
    Article Snippet: .. Labeling of the probe was performed by using a Klenow fragment (exo-) (Thermo Scientific), random hexamer primers, and biotin-11-dUTP (Jena Bioscience, Jena, Germany). .. Signals were visualized by using Poly-HRP conjugated to streptavidin and ECL Plus Western blotting substrate (both from Thermo Scientific) on a ChemiDoc MP imaging system (Bio-Rad Laboratories, Hercules, CA).

    Article Title: Xpp1 regulates the expression of xylanases, but not of cellulases in Trichoderma reesei
    Article Snippet: .. Labeling of the probe was performed using a Klenow Fragment (exo-) (Thermo Scientific), random hexamer primers, and biotin-11-dUTP (Jena Bioscience, Jena, Germany). .. Signals were visualized by using Poly-HRP conjugated to streptavidin and ECL Plus Western Blotting substrate (both Thermo Scientific Pierce, part of Life Technologies) on a ChemiDoc MP (Bio-Rad Laboratories, Hercules, USA).

    Article Title: Fusion transcription factors for strong, constitutive expression of cellulases and xylanases in Trichoderma reesei
    Article Snippet: .. Labeling of the probe was performed using a Klenow Fragment (exo-) (Thermo Scientific), random hexamer primers, and biotin-11-dUTP (Jena Bioscience, Jena, Germany). .. Signals were visualized using Poly-HRP conjugated to streptavidin and ECL Plus Western Blotting substrate (both Thermo Scientific) on a ChemiDoc MP (Bio-Rad Laboratories, Hercules, USA).

    Article Title: Influence of cis Element Arrangement on Promoter Strength in Trichoderma reesei
    Article Snippet: .. Labeling of the probe was performed by using the Klenow fragment (exo− ) (Thermo Fisher Scientific), random hexamer primers, and biotin-11-dUTP (Jena Bioscience, Jena, Germany). .. Signals were visualized by using poly-horseradish peroxidase (poly-HRP; Pierce) conjugated to streptavidin and ECL plus Western blotting substrate (Life Technologies) (both from Thermo Fisher Scientific) on a ChemiDoc MP (Bio-Rad Laboratories, Hercules, CA, USA).

    Article Title: Novel Strategies for Genomic Manipulation of Trichoderma reesei with the Purpose of Strain Engineering
    Article Snippet: .. Labeling of the probe was performed by using Klenow fragment (exo−) (Thermo Scientific), random hexamer primers, and biotin-11-dUTP (Jena Bioscience, Jena, Germany). .. Signals were visualized by using poly-horseradish peroxidase (poly-HRP) conjugated to streptavidin and ECL Plus Western blotting substrate (both from Thermo Scientific Pierce, Life Technologies) on a ChemiDoc MP (Bio-Rad Laboratories, Hercules, CA, USA).

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  • 93
    Jena Bioscience biotin 11 dutp
    Biotin 11 Dutp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin 11 dutp/product/Jena Bioscience
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    biotin 11 dutp - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

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