aminoallyl dutp atto 647n  (Jena Bioscience)


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    Name:
    Aminoallyl dUTP ATTO 647N
    Description:

    Catalog Number:
    NU-803-647N-L
    Price:
    395.34
    Category:
    Nucleotides Nucleosides
    Size:
    5 x 10 µl
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    Structured Review

    Jena Bioscience aminoallyl dutp atto 647n

    https://www.bioz.com/result/aminoallyl dutp atto 647n/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aminoallyl dutp atto 647n - by Bioz Stars, 2021-06
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Quantifying DNA damage induced by ionizing radiation and hyperthermia using single DNA molecule imaging
    Article Snippet: Fluorescent labelling of DNA damage sites Samples containing 500 ng of DNA were incubated with APE1 (5 U), FpG (1.6 U), Endo III (2 U), Endo IV (2 U) and Endo VIII (2 U) in the reaction buffer (0.05 M Tris HCl (pH 7.8), 0.1 mM dithiothreitol, 0.01 M MgSO4 , 50 μg/ml bovine serum albumin) for 2 h at 37 °C in a final volume of 100 μl. .. The enzymes were inactivated at 65 °C for 5 min, followed by 4 h incubation at 16 °C with dNTPs (1 μM of dATP, dGTP, dCTP, 0.1 μM dTTP (Sigma-Aldrich) and 0.1 μM Aminoallyl-dUTP-ATTO-647 N (Jena Bioscience), in nick translation buffer (0.05 M Tris HCl (pH 7.5), 0.1 mM dithiothreitol, 0.01 M MgSO4, 50 μg/ml bovine serum albumin), supplemented with DNA polymerase 1 (5 U), 1 mM ATP, and T4 DNA ligase (10 U). ..

    Nick Translation:

    Article Title: Quantifying DNA damage induced by ionizing radiation and hyperthermia using single DNA molecule imaging
    Article Snippet: Fluorescent labelling of DNA damage sites Samples containing 500 ng of DNA were incubated with APE1 (5 U), FpG (1.6 U), Endo III (2 U), Endo IV (2 U) and Endo VIII (2 U) in the reaction buffer (0.05 M Tris HCl (pH 7.8), 0.1 mM dithiothreitol, 0.01 M MgSO4 , 50 μg/ml bovine serum albumin) for 2 h at 37 °C in a final volume of 100 μl. .. The enzymes were inactivated at 65 °C for 5 min, followed by 4 h incubation at 16 °C with dNTPs (1 μM of dATP, dGTP, dCTP, 0.1 μM dTTP (Sigma-Aldrich) and 0.1 μM Aminoallyl-dUTP-ATTO-647 N (Jena Bioscience), in nick translation buffer (0.05 M Tris HCl (pH 7.5), 0.1 mM dithiothreitol, 0.01 M MgSO4, 50 μg/ml bovine serum albumin), supplemented with DNA polymerase 1 (5 U), 1 mM ATP, and T4 DNA ligase (10 U). ..

    Article Title: A Central Role for Canonical PRC1 in Shaping the 3D Nuclear Landscape
    Article Snippet: Hybridisation800 ng of each fluorescently labelled oligonucleotide probe pool (2 μl; MyTags or Roche Probes) were added to (26 μl) hybridisation mix (50% formamide, 2× SSC, 1% Tween20, 10% Dextran Sulphate), denatured for 5 min at 70°C and then snap chilled on ice. .. 1 μg of fosmid DNA was labelled by nick translation to incorporate green-dUTP (Enzo lifesciences), AlexaFluor 594-dUTP (Invitrogen) or aminoallyl-dUTP-ATTO-647N (Jena biosciences). .. 100 ng of fosmid, 6 μl of Cot1 DNA and 5 μg of sonicated salmon sperm DNA was dried in a spin-vac and then re-consituted in 30 μl of hybridisation mix.

    Article Title: A central role for canonical PRC1 in shaping the 3D nuclear landscape
    Article Snippet: Eight-hundred nanograms of each fluorescently labeled oligonucleotide probe pool (2 μL; MyTags or Roche Probes) was added to 26 μL of hybridization mix (50% formamide, 2× SSC, 1% Tween20, 10% dextran sulfate), denatured for 5 min at 70°C, and then snap-chilled on ice. .. One microgram of fosmid DNA was labeled by nick translation to incorporate green-dUTP (Enzo Lifesciences), Alexa fluor 594-dUTP (Invitrogen) or aminoallyl-dUTP-ATTO-647N (Jena Biosciences). .. One-hundred nanograms of fosmid, 6 μL of Cot1 DNA, and 5 μg of sonicated salmon sperm DNA were dried in a spin-vac and then reconstituted in 30 μL of hybridization mix.

    Article Title: Supernumerary B chromosomes of Aegilops speltoides undergo precise elimination in roots early in embryo development
    Article Snippet: For FISH of immunolabeled tissue sections: after immunostaining, the slides were fixed in 3:1 (ethanol: acetic acid) for 10 min and washed 2 × 5 min in 2×SSC. .. Plasmid DNA of clones AesTR-183, AesTR-205, AesTR-148 and pTa-794 was isolated with the QIAGEN Plasmid Midi Kit and labeled with dUTP-ATTO-488, dUTP-ATTO-550 or dUTP-ATTO-647N by nick-translation using the ATTO NT Labeling Kit (Jena Bioscience, Germany). ..

    Article Title: Evolution of the S-Genomes in Triticum-Aegilops Alliance: Evidences From Chromosome Analysis
    Article Snippet: .. DNA Probes Following probes were used for FISH: Plasmid clones pTa71 - a 9 kb long sequence of common wheat encoding 18S, 5.8S and 26S rRNA genes including spacers (Gerlach and Bedbrook, ), pTa794 – a 420 bp long sequence of wheat containing the 5S rRNA gene and intergenic spacer (Gerlach and Dyer, ), pAs1 - a 1 kb fragment derived from Ae. tauschii and belonging to Afa family (Rayburn and Gill, ), pSc119.2 – a 120 bp long sequence isolated from rye (Bedbrook et al., ), pTa-s53 – a 587 bp DNA fragment isolated from common wheat (Komuro et al., ), Spelt-1 – a 150 bp fragment isolated from Ae. speltoides (Salina et al., ), Spelt-52 (homolog of pAesKB52) – a 276 bp long DNA fragment isolated from Ae. speltoides (Salina et al., ), and pAesp_SAT86 - a new satellite family with a monomer length of 86 bp isolated from Ae. speltoides genomic DNA (Badaeva et al., ) and showing 91-94% similarity to wheat repeat pTa-713 described in Komuro et al. ( ) were labeled with dUTP-ATTO-488, dUTP-ATTO-550, dUTP-ATTO-647N by nick-translation using an Atto NT Labeling Kit (Jena Bioscience, Germany) or with FITC (fluorescein-12-dUTP, Roche, Germany) or biotin (biotin-16-dUTP, Roche, Germany) by nick-translation using the Nick Translation Mix (Roche, Germany) according to manufacturers' instruction. ..

    Labeling:

    Article Title: A central role for canonical PRC1 in shaping the 3D nuclear landscape
    Article Snippet: Eight-hundred nanograms of each fluorescently labeled oligonucleotide probe pool (2 μL; MyTags or Roche Probes) was added to 26 μL of hybridization mix (50% formamide, 2× SSC, 1% Tween20, 10% dextran sulfate), denatured for 5 min at 70°C, and then snap-chilled on ice. .. One microgram of fosmid DNA was labeled by nick translation to incorporate green-dUTP (Enzo Lifesciences), Alexa fluor 594-dUTP (Invitrogen) or aminoallyl-dUTP-ATTO-647N (Jena Biosciences). .. One-hundred nanograms of fosmid, 6 μL of Cot1 DNA, and 5 μg of sonicated salmon sperm DNA were dried in a spin-vac and then reconstituted in 30 μL of hybridization mix.

    Article Title: Supernumerary B chromosomes of Aegilops speltoides undergo precise elimination in roots early in embryo development
    Article Snippet: For FISH of immunolabeled tissue sections: after immunostaining, the slides were fixed in 3:1 (ethanol: acetic acid) for 10 min and washed 2 × 5 min in 2×SSC. .. Plasmid DNA of clones AesTR-183, AesTR-205, AesTR-148 and pTa-794 was isolated with the QIAGEN Plasmid Midi Kit and labeled with dUTP-ATTO-488, dUTP-ATTO-550 or dUTP-ATTO-647N by nick-translation using the ATTO NT Labeling Kit (Jena Bioscience, Germany). ..

    Article Title: Evolution of the S-Genomes in Triticum-Aegilops Alliance: Evidences From Chromosome Analysis
    Article Snippet: .. DNA Probes Following probes were used for FISH: Plasmid clones pTa71 - a 9 kb long sequence of common wheat encoding 18S, 5.8S and 26S rRNA genes including spacers (Gerlach and Bedbrook, ), pTa794 – a 420 bp long sequence of wheat containing the 5S rRNA gene and intergenic spacer (Gerlach and Dyer, ), pAs1 - a 1 kb fragment derived from Ae. tauschii and belonging to Afa family (Rayburn and Gill, ), pSc119.2 – a 120 bp long sequence isolated from rye (Bedbrook et al., ), pTa-s53 – a 587 bp DNA fragment isolated from common wheat (Komuro et al., ), Spelt-1 – a 150 bp fragment isolated from Ae. speltoides (Salina et al., ), Spelt-52 (homolog of pAesKB52) – a 276 bp long DNA fragment isolated from Ae. speltoides (Salina et al., ), and pAesp_SAT86 - a new satellite family with a monomer length of 86 bp isolated from Ae. speltoides genomic DNA (Badaeva et al., ) and showing 91-94% similarity to wheat repeat pTa-713 described in Komuro et al. ( ) were labeled with dUTP-ATTO-488, dUTP-ATTO-550, dUTP-ATTO-647N by nick-translation using an Atto NT Labeling Kit (Jena Bioscience, Germany) or with FITC (fluorescein-12-dUTP, Roche, Germany) or biotin (biotin-16-dUTP, Roche, Germany) by nick-translation using the Nick Translation Mix (Roche, Germany) according to manufacturers' instruction. ..

    Plasmid Preparation:

    Article Title: Supernumerary B chromosomes of Aegilops speltoides undergo precise elimination in roots early in embryo development
    Article Snippet: For FISH of immunolabeled tissue sections: after immunostaining, the slides were fixed in 3:1 (ethanol: acetic acid) for 10 min and washed 2 × 5 min in 2×SSC. .. Plasmid DNA of clones AesTR-183, AesTR-205, AesTR-148 and pTa-794 was isolated with the QIAGEN Plasmid Midi Kit and labeled with dUTP-ATTO-488, dUTP-ATTO-550 or dUTP-ATTO-647N by nick-translation using the ATTO NT Labeling Kit (Jena Bioscience, Germany). ..

    Article Title: Evolution of the S-Genomes in Triticum-Aegilops Alliance: Evidences From Chromosome Analysis
    Article Snippet: .. DNA Probes Following probes were used for FISH: Plasmid clones pTa71 - a 9 kb long sequence of common wheat encoding 18S, 5.8S and 26S rRNA genes including spacers (Gerlach and Bedbrook, ), pTa794 – a 420 bp long sequence of wheat containing the 5S rRNA gene and intergenic spacer (Gerlach and Dyer, ), pAs1 - a 1 kb fragment derived from Ae. tauschii and belonging to Afa family (Rayburn and Gill, ), pSc119.2 – a 120 bp long sequence isolated from rye (Bedbrook et al., ), pTa-s53 – a 587 bp DNA fragment isolated from common wheat (Komuro et al., ), Spelt-1 – a 150 bp fragment isolated from Ae. speltoides (Salina et al., ), Spelt-52 (homolog of pAesKB52) – a 276 bp long DNA fragment isolated from Ae. speltoides (Salina et al., ), and pAesp_SAT86 - a new satellite family with a monomer length of 86 bp isolated from Ae. speltoides genomic DNA (Badaeva et al., ) and showing 91-94% similarity to wheat repeat pTa-713 described in Komuro et al. ( ) were labeled with dUTP-ATTO-488, dUTP-ATTO-550, dUTP-ATTO-647N by nick-translation using an Atto NT Labeling Kit (Jena Bioscience, Germany) or with FITC (fluorescein-12-dUTP, Roche, Germany) or biotin (biotin-16-dUTP, Roche, Germany) by nick-translation using the Nick Translation Mix (Roche, Germany) according to manufacturers' instruction. ..

    Clone Assay:

    Article Title: Supernumerary B chromosomes of Aegilops speltoides undergo precise elimination in roots early in embryo development
    Article Snippet: For FISH of immunolabeled tissue sections: after immunostaining, the slides were fixed in 3:1 (ethanol: acetic acid) for 10 min and washed 2 × 5 min in 2×SSC. .. Plasmid DNA of clones AesTR-183, AesTR-205, AesTR-148 and pTa-794 was isolated with the QIAGEN Plasmid Midi Kit and labeled with dUTP-ATTO-488, dUTP-ATTO-550 or dUTP-ATTO-647N by nick-translation using the ATTO NT Labeling Kit (Jena Bioscience, Germany). ..

    Article Title: Evolution of the S-Genomes in Triticum-Aegilops Alliance: Evidences From Chromosome Analysis
    Article Snippet: .. DNA Probes Following probes were used for FISH: Plasmid clones pTa71 - a 9 kb long sequence of common wheat encoding 18S, 5.8S and 26S rRNA genes including spacers (Gerlach and Bedbrook, ), pTa794 – a 420 bp long sequence of wheat containing the 5S rRNA gene and intergenic spacer (Gerlach and Dyer, ), pAs1 - a 1 kb fragment derived from Ae. tauschii and belonging to Afa family (Rayburn and Gill, ), pSc119.2 – a 120 bp long sequence isolated from rye (Bedbrook et al., ), pTa-s53 – a 587 bp DNA fragment isolated from common wheat (Komuro et al., ), Spelt-1 – a 150 bp fragment isolated from Ae. speltoides (Salina et al., ), Spelt-52 (homolog of pAesKB52) – a 276 bp long DNA fragment isolated from Ae. speltoides (Salina et al., ), and pAesp_SAT86 - a new satellite family with a monomer length of 86 bp isolated from Ae. speltoides genomic DNA (Badaeva et al., ) and showing 91-94% similarity to wheat repeat pTa-713 described in Komuro et al. ( ) were labeled with dUTP-ATTO-488, dUTP-ATTO-550, dUTP-ATTO-647N by nick-translation using an Atto NT Labeling Kit (Jena Bioscience, Germany) or with FITC (fluorescein-12-dUTP, Roche, Germany) or biotin (biotin-16-dUTP, Roche, Germany) by nick-translation using the Nick Translation Mix (Roche, Germany) according to manufacturers' instruction. ..

    Isolation:

    Article Title: Supernumerary B chromosomes of Aegilops speltoides undergo precise elimination in roots early in embryo development
    Article Snippet: For FISH of immunolabeled tissue sections: after immunostaining, the slides were fixed in 3:1 (ethanol: acetic acid) for 10 min and washed 2 × 5 min in 2×SSC. .. Plasmid DNA of clones AesTR-183, AesTR-205, AesTR-148 and pTa-794 was isolated with the QIAGEN Plasmid Midi Kit and labeled with dUTP-ATTO-488, dUTP-ATTO-550 or dUTP-ATTO-647N by nick-translation using the ATTO NT Labeling Kit (Jena Bioscience, Germany). ..

    Article Title: Evolution of the S-Genomes in Triticum-Aegilops Alliance: Evidences From Chromosome Analysis
    Article Snippet: .. DNA Probes Following probes were used for FISH: Plasmid clones pTa71 - a 9 kb long sequence of common wheat encoding 18S, 5.8S and 26S rRNA genes including spacers (Gerlach and Bedbrook, ), pTa794 – a 420 bp long sequence of wheat containing the 5S rRNA gene and intergenic spacer (Gerlach and Dyer, ), pAs1 - a 1 kb fragment derived from Ae. tauschii and belonging to Afa family (Rayburn and Gill, ), pSc119.2 – a 120 bp long sequence isolated from rye (Bedbrook et al., ), pTa-s53 – a 587 bp DNA fragment isolated from common wheat (Komuro et al., ), Spelt-1 – a 150 bp fragment isolated from Ae. speltoides (Salina et al., ), Spelt-52 (homolog of pAesKB52) – a 276 bp long DNA fragment isolated from Ae. speltoides (Salina et al., ), and pAesp_SAT86 - a new satellite family with a monomer length of 86 bp isolated from Ae. speltoides genomic DNA (Badaeva et al., ) and showing 91-94% similarity to wheat repeat pTa-713 described in Komuro et al. ( ) were labeled with dUTP-ATTO-488, dUTP-ATTO-550, dUTP-ATTO-647N by nick-translation using an Atto NT Labeling Kit (Jena Bioscience, Germany) or with FITC (fluorescein-12-dUTP, Roche, Germany) or biotin (biotin-16-dUTP, Roche, Germany) by nick-translation using the Nick Translation Mix (Roche, Germany) according to manufacturers' instruction. ..

    Fluorescence In Situ Hybridization:

    Article Title: Evolution of the S-Genomes in Triticum-Aegilops Alliance: Evidences From Chromosome Analysis
    Article Snippet: .. DNA Probes Following probes were used for FISH: Plasmid clones pTa71 - a 9 kb long sequence of common wheat encoding 18S, 5.8S and 26S rRNA genes including spacers (Gerlach and Bedbrook, ), pTa794 – a 420 bp long sequence of wheat containing the 5S rRNA gene and intergenic spacer (Gerlach and Dyer, ), pAs1 - a 1 kb fragment derived from Ae. tauschii and belonging to Afa family (Rayburn and Gill, ), pSc119.2 – a 120 bp long sequence isolated from rye (Bedbrook et al., ), pTa-s53 – a 587 bp DNA fragment isolated from common wheat (Komuro et al., ), Spelt-1 – a 150 bp fragment isolated from Ae. speltoides (Salina et al., ), Spelt-52 (homolog of pAesKB52) – a 276 bp long DNA fragment isolated from Ae. speltoides (Salina et al., ), and pAesp_SAT86 - a new satellite family with a monomer length of 86 bp isolated from Ae. speltoides genomic DNA (Badaeva et al., ) and showing 91-94% similarity to wheat repeat pTa-713 described in Komuro et al. ( ) were labeled with dUTP-ATTO-488, dUTP-ATTO-550, dUTP-ATTO-647N by nick-translation using an Atto NT Labeling Kit (Jena Bioscience, Germany) or with FITC (fluorescein-12-dUTP, Roche, Germany) or biotin (biotin-16-dUTP, Roche, Germany) by nick-translation using the Nick Translation Mix (Roche, Germany) according to manufacturers' instruction. ..

    Sequencing:

    Article Title: Evolution of the S-Genomes in Triticum-Aegilops Alliance: Evidences From Chromosome Analysis
    Article Snippet: .. DNA Probes Following probes were used for FISH: Plasmid clones pTa71 - a 9 kb long sequence of common wheat encoding 18S, 5.8S and 26S rRNA genes including spacers (Gerlach and Bedbrook, ), pTa794 – a 420 bp long sequence of wheat containing the 5S rRNA gene and intergenic spacer (Gerlach and Dyer, ), pAs1 - a 1 kb fragment derived from Ae. tauschii and belonging to Afa family (Rayburn and Gill, ), pSc119.2 – a 120 bp long sequence isolated from rye (Bedbrook et al., ), pTa-s53 – a 587 bp DNA fragment isolated from common wheat (Komuro et al., ), Spelt-1 – a 150 bp fragment isolated from Ae. speltoides (Salina et al., ), Spelt-52 (homolog of pAesKB52) – a 276 bp long DNA fragment isolated from Ae. speltoides (Salina et al., ), and pAesp_SAT86 - a new satellite family with a monomer length of 86 bp isolated from Ae. speltoides genomic DNA (Badaeva et al., ) and showing 91-94% similarity to wheat repeat pTa-713 described in Komuro et al. ( ) were labeled with dUTP-ATTO-488, dUTP-ATTO-550, dUTP-ATTO-647N by nick-translation using an Atto NT Labeling Kit (Jena Bioscience, Germany) or with FITC (fluorescein-12-dUTP, Roche, Germany) or biotin (biotin-16-dUTP, Roche, Germany) by nick-translation using the Nick Translation Mix (Roche, Germany) according to manufacturers' instruction. ..

    Derivative Assay:

    Article Title: Evolution of the S-Genomes in Triticum-Aegilops Alliance: Evidences From Chromosome Analysis
    Article Snippet: .. DNA Probes Following probes were used for FISH: Plasmid clones pTa71 - a 9 kb long sequence of common wheat encoding 18S, 5.8S and 26S rRNA genes including spacers (Gerlach and Bedbrook, ), pTa794 – a 420 bp long sequence of wheat containing the 5S rRNA gene and intergenic spacer (Gerlach and Dyer, ), pAs1 - a 1 kb fragment derived from Ae. tauschii and belonging to Afa family (Rayburn and Gill, ), pSc119.2 – a 120 bp long sequence isolated from rye (Bedbrook et al., ), pTa-s53 – a 587 bp DNA fragment isolated from common wheat (Komuro et al., ), Spelt-1 – a 150 bp fragment isolated from Ae. speltoides (Salina et al., ), Spelt-52 (homolog of pAesKB52) – a 276 bp long DNA fragment isolated from Ae. speltoides (Salina et al., ), and pAesp_SAT86 - a new satellite family with a monomer length of 86 bp isolated from Ae. speltoides genomic DNA (Badaeva et al., ) and showing 91-94% similarity to wheat repeat pTa-713 described in Komuro et al. ( ) were labeled with dUTP-ATTO-488, dUTP-ATTO-550, dUTP-ATTO-647N by nick-translation using an Atto NT Labeling Kit (Jena Bioscience, Germany) or with FITC (fluorescein-12-dUTP, Roche, Germany) or biotin (biotin-16-dUTP, Roche, Germany) by nick-translation using the Nick Translation Mix (Roche, Germany) according to manufacturers' instruction. ..

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  • 93
    Jena Bioscience aminoallyl dutp atto 647n
    Aminoallyl Dutp Atto 647n, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aminoallyl dutp atto 647n/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aminoallyl dutp atto 647n - by Bioz Stars, 2021-06
    93/100 stars
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