fluorescein 12 dutp  (Jena Bioscience)


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  • 94
    Name:
    Fluorescein 12 dUTP
    Description:
    Fluorescein 12 dUTP is recommended for direct enzymatic labeling of DNA cDNA e g by PCR and Nick Translation It is incorporated as substitute for its natural counterpart dTTP The resulting Dye labeled DNA cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray based gene expression profiling Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine Recommended Fluorescein 12 dUTP dTTP ratio for PCR and Nick Translation 30 50 Fluorescein 12 dUTP 70 50 dTTP Please note Protect the Dye labeled dUTP from exposure to light and carry out experimental procedures in low light conditions The optimal final concentration of the Dye labeled dUTP may very depending on the application and assay conditions For optimal product yields and high incorporation rates an individual optimization of the Dye labeled dUTP dTTP ratio is recommended
    Catalog Number:
    nu-803-famx-l
    Price:
    243.6
    Applications:
    Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data, [1] - Nick Translation with DNAse I/ DNA Polymerase I in-house data
    Purity:
    ≥ 95 % (HPLC)
    Category:
    Nucleotides Nucleosides
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    Structured Review

    Jena Bioscience fluorescein 12 dutp
    Fluorescein 12 dUTP
    Fluorescein 12 dUTP is recommended for direct enzymatic labeling of DNA cDNA e g by PCR and Nick Translation It is incorporated as substitute for its natural counterpart dTTP The resulting Dye labeled DNA cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray based gene expression profiling Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine Recommended Fluorescein 12 dUTP dTTP ratio for PCR and Nick Translation 30 50 Fluorescein 12 dUTP 70 50 dTTP Please note Protect the Dye labeled dUTP from exposure to light and carry out experimental procedures in low light conditions The optimal final concentration of the Dye labeled dUTP may very depending on the application and assay conditions For optimal product yields and high incorporation rates an individual optimization of the Dye labeled dUTP dTTP ratio is recommended
    https://www.bioz.com/result/fluorescein 12 dutp/product/Jena Bioscience
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    fluorescein 12 dutp - by Bioz Stars, 2020-09
    94/100 stars

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    Related Articles

    Nick Translation:

    Article Title: Insights into the Structure of the Spruce Budworm (Choristoneura fumiferana) Genome, as Revealed by Molecular Cytogenetic Analyses and a High-Density Linkage Map
    Article Snippet: .. Female gDNA was labeled with fluorescein-12-dUTP (Jena Bioscience) using the Nick Translation Kit (Abbott Molecular Inc.), with 4 h of incubation at 15°, and male gDNA was sonicated using a Sonopuls HD 2070 (Bandelin Electric, Berlin, Germany) and used as competitor DNA. .. For GISH combined with tel-FISH, the probe mixture contained fluorescein-labeled female gDNA (300 ng), Cy3-labeled telomeric probe (100 ng), sonicated male gDNA (3 μg) and sonicated salmon sperm DNA (25 μg).

    Article Title: Neo-sex Chromosomes in the Monarch Butterfly, Danaus plexippus
    Article Snippet: .. Hybridization probes were prepared from the entire complement of extracted male or female gDNA, which was labeled by aminoallyl-dUTP-Cy3 or fluorescein-12-dUTP (both Jena Bioscience GmbH, Jena, Germany) using the Nick Translation Kit (Abbott Molecular Inc., Des Plaines, IL) with 7 hr incubation at 15° ( ). ..

    Article Title: Sex Chromosomes of the Iconic Moth Abraxas grossulariata (Lepidoptera, Geometridae) and Its Congener A. sylvata
    Article Snippet: .. The 25 μL nick translation reaction contained 500 ng gDNA; 25 µM dATP, dCTP, and dGTP; 9 µM dTTP; 16 µM labeled nucleotides with either Cy3-dUTP (male gDNA) or fluorescein-12-dUTP (female gDNA) (both Jena Bioscience, Jena, Germany); 1× nick translation buffer and 5 µL of nick translation enzyme mix. .. The reaction was incubated at 15 °C for 6–7 h. CGH was performed according to a published protocol [ ] with modifications described previously [ ].

    Labeling:

    Article Title: Insights into the Structure of the Spruce Budworm (Choristoneura fumiferana) Genome, as Revealed by Molecular Cytogenetic Analyses and a High-Density Linkage Map
    Article Snippet: .. Female gDNA was labeled with fluorescein-12-dUTP (Jena Bioscience) using the Nick Translation Kit (Abbott Molecular Inc.), with 4 h of incubation at 15°, and male gDNA was sonicated using a Sonopuls HD 2070 (Bandelin Electric, Berlin, Germany) and used as competitor DNA. .. For GISH combined with tel-FISH, the probe mixture contained fluorescein-labeled female gDNA (300 ng), Cy3-labeled telomeric probe (100 ng), sonicated male gDNA (3 μg) and sonicated salmon sperm DNA (25 μg).

    Article Title: Intraspecific and intraorganismal copy number dynamics of retrotransposons and tandem repeat in Aegilops speltoides Tausch (Poaceae, Triticeae).
    Article Snippet: .. Transposable elements (TE) and tandem repeats (TR) compose the largest fraction of the plant genome. .. Transposable elements (TE) and tandem repeats (TR) compose the largest fraction of the plant genome.

    Article Title: Repetitive DNA in the Architecture, Repatterning, and Diversification of the Genome of Aegilops speltoides Tausch (Poaceae, Triticeae)
    Article Snippet: .. The DNA probes were directly labeled with Cy-3, Fluorescein-12-dUTP, and ATTO-425 (Jena Bioscience, Germany). .. AT-specific 4′,6-diamidino-2-phenylindole (DAPI) fluorochrome was used for differential staining.

    Article Title: Transposable Elements in the Organization and Diversification of the Genome of Aegilops speltoides Tausch (Poaceae, Triticeae)
    Article Snippet: .. The DNA probes were directly labeled with Cy-3, fluorescein-12-dUTP, and ATTO-425 (Jena Bioscience, Germany). .. AT-specific 4′,6-diamidino-2-phenylindole (DAPI) and GC-specific chromomycin A3 (CMA3 ) fluorochromes were used for differential staining to reveal AT-enriched heterochromatin patterns and GC-enriched heterochromatic clusters in the nuclear organizer regions (NORs) on chromosomes 1 and 6 in the Ae. speltoides genome.

    Article Title: Neo-sex Chromosomes in the Monarch Butterfly, Danaus plexippus
    Article Snippet: .. Hybridization probes were prepared from the entire complement of extracted male or female gDNA, which was labeled by aminoallyl-dUTP-Cy3 or fluorescein-12-dUTP (both Jena Bioscience GmbH, Jena, Germany) using the Nick Translation Kit (Abbott Molecular Inc., Des Plaines, IL) with 7 hr incubation at 15° ( ). ..

    Article Title: Sex Chromosomes of the Iconic Moth Abraxas grossulariata (Lepidoptera, Geometridae) and Its Congener A. sylvata
    Article Snippet: .. The 25 μL nick translation reaction contained 500 ng gDNA; 25 µM dATP, dCTP, and dGTP; 9 µM dTTP; 16 µM labeled nucleotides with either Cy3-dUTP (male gDNA) or fluorescein-12-dUTP (female gDNA) (both Jena Bioscience, Jena, Germany); 1× nick translation buffer and 5 µL of nick translation enzyme mix. .. The reaction was incubated at 15 °C for 6–7 h. CGH was performed according to a published protocol [ ] with modifications described previously [ ].

    Article Title: Genotype- and Cell-Specific Dynamics of Tandem Repeat Patterns in Aegilops speltoides Tausch (Poaceae, Triticeae).
    Article Snippet: .. In wild plant populations, chromosome rearrangements lead to the wide intraspecific polymorphisms in the abundance and patterns of highly repetitive DNA. .. In wild plant populations, chromosome rearrangements lead to the wide intraspecific polymorphisms in the abundance and patterns of highly repetitive DNA.

    Oligonucleotide Labeling:

    Article Title: Intraspecific and intraorganismal copy number dynamics of retrotransposons and tandem repeat in Aegilops speltoides Tausch (Poaceae, Triticeae).
    Article Snippet: .. Transposable elements (TE) and tandem repeats (TR) compose the largest fraction of the plant genome. .. Transposable elements (TE) and tandem repeats (TR) compose the largest fraction of the plant genome.

    Incubation:

    Article Title: Insights into the Structure of the Spruce Budworm (Choristoneura fumiferana) Genome, as Revealed by Molecular Cytogenetic Analyses and a High-Density Linkage Map
    Article Snippet: .. Female gDNA was labeled with fluorescein-12-dUTP (Jena Bioscience) using the Nick Translation Kit (Abbott Molecular Inc.), with 4 h of incubation at 15°, and male gDNA was sonicated using a Sonopuls HD 2070 (Bandelin Electric, Berlin, Germany) and used as competitor DNA. .. For GISH combined with tel-FISH, the probe mixture contained fluorescein-labeled female gDNA (300 ng), Cy3-labeled telomeric probe (100 ng), sonicated male gDNA (3 μg) and sonicated salmon sperm DNA (25 μg).

    Article Title: Neo-sex Chromosomes in the Monarch Butterfly, Danaus plexippus
    Article Snippet: .. Hybridization probes were prepared from the entire complement of extracted male or female gDNA, which was labeled by aminoallyl-dUTP-Cy3 or fluorescein-12-dUTP (both Jena Bioscience GmbH, Jena, Germany) using the Nick Translation Kit (Abbott Molecular Inc., Des Plaines, IL) with 7 hr incubation at 15° ( ). ..

    Sonication:

    Article Title: Insights into the Structure of the Spruce Budworm (Choristoneura fumiferana) Genome, as Revealed by Molecular Cytogenetic Analyses and a High-Density Linkage Map
    Article Snippet: .. Female gDNA was labeled with fluorescein-12-dUTP (Jena Bioscience) using the Nick Translation Kit (Abbott Molecular Inc.), with 4 h of incubation at 15°, and male gDNA was sonicated using a Sonopuls HD 2070 (Bandelin Electric, Berlin, Germany) and used as competitor DNA. .. For GISH combined with tel-FISH, the probe mixture contained fluorescein-labeled female gDNA (300 ng), Cy3-labeled telomeric probe (100 ng), sonicated male gDNA (3 μg) and sonicated salmon sperm DNA (25 μg).

    Hybridization:

    Article Title: Neo-sex Chromosomes in the Monarch Butterfly, Danaus plexippus
    Article Snippet: .. Hybridization probes were prepared from the entire complement of extracted male or female gDNA, which was labeled by aminoallyl-dUTP-Cy3 or fluorescein-12-dUTP (both Jena Bioscience GmbH, Jena, Germany) using the Nick Translation Kit (Abbott Molecular Inc., Des Plaines, IL) with 7 hr incubation at 15° ( ). ..