in vitrocell es co2 incubator  (NuAire)


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    Name:
    In VitroCell ES CO2 Incubator
    Description:
    The NuAire Direct Heat DH Automatic CO2 Direct Heat Incubator has been designed to provide a reliable controlled in vitro environment for optimum tissue cell culture growth The chamber also provides an environment for the storage and preservation of embryos gametes and animal tissue cell cultures intended for research at or near body temperature
    Catalog Number:
    NU-5800
    Product Aliases:
    CO2 Incubator, Microbiological Incubator, Direct Heat
    Price:
    None
    Size:
    7 ft3 200 Liter
    Category:
    CO2 Incubator
    Score:
    85
    Buy from Supplier


    Structured Review

    NuAire in vitrocell es co2 incubator
    In VitroCell ES CO2 Incubator
    The NuAire Direct Heat DH Automatic CO2 Direct Heat Incubator has been designed to provide a reliable controlled in vitro environment for optimum tissue cell culture growth The chamber also provides an environment for the storage and preservation of embryos gametes and animal tissue cell cultures intended for research at or near body temperature
    https://www.bioz.com/result/in vitrocell es co2 incubator/product/NuAire
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    in vitrocell es co2 incubator - by Bioz Stars, 2019-10
    96/100 stars

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    Related Articles

    Filtration:

    Article Title: Isolation of Exosomes from Semen for in vitro Uptake and HIV-1 Infection Assays
    Article Snippet: This protocol lays the groundwork for evaluating other functional activities of semen exosomes, and for evaluating exosomes from other sources. .. Pipettes (any brand) Sterile tweezers (any brand) Centrifuge (Eppendorf, model: 5415 D) Ultracentrifuge (Beckman Coulter, model: Optima L-90K) SW40Ti rotor (Beckman Coulter, catalog number: 331362) Polyallomer centrifuge tubes 14 × 89 mm for SW41Ti rotor (Beckman Coulter, catalog number: 344059) 37 °C, 5% CO2 cell culture incubator (NuAire, model: NU-5510) FACSCalibur flow cytometer (BD) (see Notes for laser and filter specifications) FACSVerse flow cytometer (BD) (see Notes for laser and filter specifications) FACSAria flow cytometer (BD) (see Notes for laser and filter specifications) Laser scanning confocal microscope (Nikon Instruments, model: Eclipse TE2000) Magnetic separator for 1.7 ml tubes Rotating mixer Microplate reader (Tecan Trading, model: Infinite® M200 Pro) DynaPro Nanostar (Wyatt Technologies, model: DynaPro Nanostar) NanoSight LM10 (Malvern Instruments, model: NanoSight LM10) NanoDrop spectrophotometer (Thermo Fisher Scientific) SW60 Ti rotor (Beckman Coulter, catalog number: 335649) Polyallomer centrifuge tubes 11 × 60 mm for SW60Ti rotor (Beckman Coulter, catalog number: 355636) 7500 fast real-time PCR system (Thermo Fisher Scientific, model: 7500 Fast Real-time PCR System) Luminometer (BioTek Instruments, model: Synergy H1 Hybrid Reader) Laminar flow hood SW32 Ti rotor (Beckman Coulter, model: 369650) Polyallomer centrifuge tubes 25 × 89 mm for SW32Ti rotor (Beckman Coulter, catalog number: 344058) pH meter (any brand) Gel running tank(Thermo Fisher Scientific, Novex(x22122), model: XCell SureLock ® Mini-Cell, catalog number: EI0001) Sephacryl S300-HR 16/60 gel filtration prepacked column (GE Healthcare catalog number: 17-1167-01) BioLogic DuoFlow Workstation (Bio-Rad Laboratories) BioLogic BioFrac fraction collector (Bio-Rad Laboratories) Gel electrophoresis horizontal apparatus (Bio-Rad Laboratories, model: Wide Mini-Sub Cell GT Cell, catalog number: 1704468EDU) Dry Block Heater for microcentrifuge tubes (Thermo Fisher Scientific) .. FlowJo analysis software (TreeStar) BioLogic DuoFlow software version 5.3 (Bio-Rad Laboratories) Dynamics software (Wyatt Technology) NTA software (Malvern Instruments)

    Cytometry:

    Article Title: Isolation of Exosomes from Semen for in vitro Uptake and HIV-1 Infection Assays
    Article Snippet: This protocol lays the groundwork for evaluating other functional activities of semen exosomes, and for evaluating exosomes from other sources. .. Pipettes (any brand) Sterile tweezers (any brand) Centrifuge (Eppendorf, model: 5415 D) Ultracentrifuge (Beckman Coulter, model: Optima L-90K) SW40Ti rotor (Beckman Coulter, catalog number: 331362) Polyallomer centrifuge tubes 14 × 89 mm for SW41Ti rotor (Beckman Coulter, catalog number: 344059) 37 °C, 5% CO2 cell culture incubator (NuAire, model: NU-5510) FACSCalibur flow cytometer (BD) (see Notes for laser and filter specifications) FACSVerse flow cytometer (BD) (see Notes for laser and filter specifications) FACSAria flow cytometer (BD) (see Notes for laser and filter specifications) Laser scanning confocal microscope (Nikon Instruments, model: Eclipse TE2000) Magnetic separator for 1.7 ml tubes Rotating mixer Microplate reader (Tecan Trading, model: Infinite® M200 Pro) DynaPro Nanostar (Wyatt Technologies, model: DynaPro Nanostar) NanoSight LM10 (Malvern Instruments, model: NanoSight LM10) NanoDrop spectrophotometer (Thermo Fisher Scientific) SW60 Ti rotor (Beckman Coulter, catalog number: 335649) Polyallomer centrifuge tubes 11 × 60 mm for SW60Ti rotor (Beckman Coulter, catalog number: 355636) 7500 fast real-time PCR system (Thermo Fisher Scientific, model: 7500 Fast Real-time PCR System) Luminometer (BioTek Instruments, model: Synergy H1 Hybrid Reader) Laminar flow hood SW32 Ti rotor (Beckman Coulter, model: 369650) Polyallomer centrifuge tubes 25 × 89 mm for SW32Ti rotor (Beckman Coulter, catalog number: 344058) pH meter (any brand) Gel running tank(Thermo Fisher Scientific, Novex(x22122), model: XCell SureLock ® Mini-Cell, catalog number: EI0001) Sephacryl S300-HR 16/60 gel filtration prepacked column (GE Healthcare catalog number: 17-1167-01) BioLogic DuoFlow Workstation (Bio-Rad Laboratories) BioLogic BioFrac fraction collector (Bio-Rad Laboratories) Gel electrophoresis horizontal apparatus (Bio-Rad Laboratories, model: Wide Mini-Sub Cell GT Cell, catalog number: 1704468EDU) Dry Block Heater for microcentrifuge tubes (Thermo Fisher Scientific) .. FlowJo analysis software (TreeStar) BioLogic DuoFlow software version 5.3 (Bio-Rad Laboratories) Dynamics software (Wyatt Technology) NTA software (Malvern Instruments)

    Article Title: Defining human dendritic cell progenitors by multiparametric flow cytometry
    Article Snippet: BD CompBeads set anti-mouse Igκ (BD Biosciences, cat. no. 552843) BD CompBeads set anti-rat Igκ (BD Biosciences, cat. no. 552844) Amine-reactive compensation bead kit (Life Technologies, cat. no. ) .. Biological safety level 2 (BSL-2) tissue culture facilities BSL-2 sorting facilities Class II biological safety cabinet (The Baker Company, cat. no. SG403) Cell incubator (37 °C, 5% CO2 ; Nuaire, cat. no. NU-5500) Tabletop centrifuge with rotors and adapters for 50- and 15-ml conical tubes and 96-well plates (Sorvall, cat. no. 75-004-367) Microcentrifuge (Eppendorf, cat. no. 5424) Inverted microscope (Nikon, cat. no. Eclipse TS100) Hemocytometer (Fisher Scientific, cat. no. 02-671-5) or other cell counter Cryo 1 °C freezing container (Nalgene, cat. no. 5100-0001) LN2 storage systems (Thermo Scientific, cat. no. 7404) BD LSR II flow cytometer running DIVA 8.0, or equivalent (BD Biosciences, special order). .. Our LSR II is equipped with four lasers with a configuration allowing 16-color, 18-parameter flow cytometry ( ) BD Aria III cell sorter running DIVA 8.0, or equivalent (BD Biosciences, special order).

    Blocking Assay:

    Article Title: Isolation of Exosomes from Semen for in vitro Uptake and HIV-1 Infection Assays
    Article Snippet: This protocol lays the groundwork for evaluating other functional activities of semen exosomes, and for evaluating exosomes from other sources. .. Pipettes (any brand) Sterile tweezers (any brand) Centrifuge (Eppendorf, model: 5415 D) Ultracentrifuge (Beckman Coulter, model: Optima L-90K) SW40Ti rotor (Beckman Coulter, catalog number: 331362) Polyallomer centrifuge tubes 14 × 89 mm for SW41Ti rotor (Beckman Coulter, catalog number: 344059) 37 °C, 5% CO2 cell culture incubator (NuAire, model: NU-5510) FACSCalibur flow cytometer (BD) (see Notes for laser and filter specifications) FACSVerse flow cytometer (BD) (see Notes for laser and filter specifications) FACSAria flow cytometer (BD) (see Notes for laser and filter specifications) Laser scanning confocal microscope (Nikon Instruments, model: Eclipse TE2000) Magnetic separator for 1.7 ml tubes Rotating mixer Microplate reader (Tecan Trading, model: Infinite® M200 Pro) DynaPro Nanostar (Wyatt Technologies, model: DynaPro Nanostar) NanoSight LM10 (Malvern Instruments, model: NanoSight LM10) NanoDrop spectrophotometer (Thermo Fisher Scientific) SW60 Ti rotor (Beckman Coulter, catalog number: 335649) Polyallomer centrifuge tubes 11 × 60 mm for SW60Ti rotor (Beckman Coulter, catalog number: 355636) 7500 fast real-time PCR system (Thermo Fisher Scientific, model: 7500 Fast Real-time PCR System) Luminometer (BioTek Instruments, model: Synergy H1 Hybrid Reader) Laminar flow hood SW32 Ti rotor (Beckman Coulter, model: 369650) Polyallomer centrifuge tubes 25 × 89 mm for SW32Ti rotor (Beckman Coulter, catalog number: 344058) pH meter (any brand) Gel running tank(Thermo Fisher Scientific, Novex(x22122), model: XCell SureLock ® Mini-Cell, catalog number: EI0001) Sephacryl S300-HR 16/60 gel filtration prepacked column (GE Healthcare catalog number: 17-1167-01) BioLogic DuoFlow Workstation (Bio-Rad Laboratories) BioLogic BioFrac fraction collector (Bio-Rad Laboratories) Gel electrophoresis horizontal apparatus (Bio-Rad Laboratories, model: Wide Mini-Sub Cell GT Cell, catalog number: 1704468EDU) Dry Block Heater for microcentrifuge tubes (Thermo Fisher Scientific) .. FlowJo analysis software (TreeStar) BioLogic DuoFlow software version 5.3 (Bio-Rad Laboratories) Dynamics software (Wyatt Technology) NTA software (Malvern Instruments)

    Article Title: Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment
    Article Snippet: The quantity of the specific protein band in the lanes was analyzed using the ImageJ (version 1.50; National Institutes of Health, Bethesda, MD, USA) program. .. SK-N-MC cells were seeded at a density of 1×104 cells/well in a 24-well flat-bottom tissue culture microtiter plate pretreated with gelatin for 12 h; then, 10 µM of fibrillary Aβ1–42 and fluorescent LIP carriers were added, and the SK-N-MC cells were cultured in a humidified CO2 incubator for 24 h. The culture of SK-N-MC cells was fixed with 0.5 mL of 10% (v/v) formalin at 25°C for 5 min, immersed in 0.5% (v/v) Triton-X-100 at 25°C for 10 min, treated with 200 µL of CAS block at 25°C for 10 min, reacted with antiphospho-protein antibody (anti-p-p38, 1:800; anti-p-JNK, 1:200; and anti-p-ERK5, 1:200) at 4°C for 12 h, incubated with goat anti-rabbit IgG (H & L, HRP) with rhodamine conjugate (1:200) at 25°C for 1 h in darkness, counterstained with 0.5% (w/v) DAPI in 0.1% (v/v) Triton-X-100 at 25°C for 3 min in darkness, and incubated with aqueous mounting medium. .. The fluorescent staining was visualized using a confocal laser scanning microscope (LSM 510; Zeiss, Oberkochen, Germany) at excitation of 350 nm (blue), 490 nm (green), and 540 nm (red) and emission of 475 nm (blue), 520 nm (green), and 565 nm (red).

    Article Title: Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin
    Article Snippet: The ability of QU to cross the BBB, P BBB,QU , was defined as ([ability of QU to cross the membrane with HBMEC/HA]−1 − [ability of QU to cross the membrane without cells]−1 )−1 . .. HBMECs at a density of 1×105 cells/cm2 were seeded on a microscope cover glass in a 24-well plate, cultured in a humidified CO2 incubator for 8 h, treated with 0.025% (w/v) RMP-7-Lf-QU-LS, and cultured in a humidified CO2 incubator for 3 h. In preparing the staining sample, cultured HBMECs were fixed with 10% (v/v) formalin at 25°C for 10 min, treated with 0.5% (v/v) Triton-X-100 at 25°C for 10 min, and incubated with serum blocking solution at 25°C for 30 min. .. The sample was reacted with 1 μg/mL of anti-LfR monoclonal antibody and/or with anti-B2 bradykinin receptor antibody (1:1,000) at 4°C for 12 h, stained with goat anti-mouse polyclonal secondary antibody IgG (H + L) conjugated with rhodamine (1:100) at 25°C for 1 h in darkness, treated with 0.5% (w/v) DAPI in 0.1% (v/v) Triton-X-100 at 25°C for 3 min in darkness, and incubated in an aqueous mounting medium.

    Real-time Polymerase Chain Reaction:

    Article Title: Isolation of Exosomes from Semen for in vitro Uptake and HIV-1 Infection Assays
    Article Snippet: This protocol lays the groundwork for evaluating other functional activities of semen exosomes, and for evaluating exosomes from other sources. .. Pipettes (any brand) Sterile tweezers (any brand) Centrifuge (Eppendorf, model: 5415 D) Ultracentrifuge (Beckman Coulter, model: Optima L-90K) SW40Ti rotor (Beckman Coulter, catalog number: 331362) Polyallomer centrifuge tubes 14 × 89 mm for SW41Ti rotor (Beckman Coulter, catalog number: 344059) 37 °C, 5% CO2 cell culture incubator (NuAire, model: NU-5510) FACSCalibur flow cytometer (BD) (see Notes for laser and filter specifications) FACSVerse flow cytometer (BD) (see Notes for laser and filter specifications) FACSAria flow cytometer (BD) (see Notes for laser and filter specifications) Laser scanning confocal microscope (Nikon Instruments, model: Eclipse TE2000) Magnetic separator for 1.7 ml tubes Rotating mixer Microplate reader (Tecan Trading, model: Infinite® M200 Pro) DynaPro Nanostar (Wyatt Technologies, model: DynaPro Nanostar) NanoSight LM10 (Malvern Instruments, model: NanoSight LM10) NanoDrop spectrophotometer (Thermo Fisher Scientific) SW60 Ti rotor (Beckman Coulter, catalog number: 335649) Polyallomer centrifuge tubes 11 × 60 mm for SW60Ti rotor (Beckman Coulter, catalog number: 355636) 7500 fast real-time PCR system (Thermo Fisher Scientific, model: 7500 Fast Real-time PCR System) Luminometer (BioTek Instruments, model: Synergy H1 Hybrid Reader) Laminar flow hood SW32 Ti rotor (Beckman Coulter, model: 369650) Polyallomer centrifuge tubes 25 × 89 mm for SW32Ti rotor (Beckman Coulter, catalog number: 344058) pH meter (any brand) Gel running tank(Thermo Fisher Scientific, Novex(x22122), model: XCell SureLock ® Mini-Cell, catalog number: EI0001) Sephacryl S300-HR 16/60 gel filtration prepacked column (GE Healthcare catalog number: 17-1167-01) BioLogic DuoFlow Workstation (Bio-Rad Laboratories) BioLogic BioFrac fraction collector (Bio-Rad Laboratories) Gel electrophoresis horizontal apparatus (Bio-Rad Laboratories, model: Wide Mini-Sub Cell GT Cell, catalog number: 1704468EDU) Dry Block Heater for microcentrifuge tubes (Thermo Fisher Scientific) .. FlowJo analysis software (TreeStar) BioLogic DuoFlow software version 5.3 (Bio-Rad Laboratories) Dynamics software (Wyatt Technology) NTA software (Malvern Instruments)

    Incubation:

    Article Title: Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment
    Article Snippet: The quantity of the specific protein band in the lanes was analyzed using the ImageJ (version 1.50; National Institutes of Health, Bethesda, MD, USA) program. .. SK-N-MC cells were seeded at a density of 1×104 cells/well in a 24-well flat-bottom tissue culture microtiter plate pretreated with gelatin for 12 h; then, 10 µM of fibrillary Aβ1–42 and fluorescent LIP carriers were added, and the SK-N-MC cells were cultured in a humidified CO2 incubator for 24 h. The culture of SK-N-MC cells was fixed with 0.5 mL of 10% (v/v) formalin at 25°C for 5 min, immersed in 0.5% (v/v) Triton-X-100 at 25°C for 10 min, treated with 200 µL of CAS block at 25°C for 10 min, reacted with antiphospho-protein antibody (anti-p-p38, 1:800; anti-p-JNK, 1:200; and anti-p-ERK5, 1:200) at 4°C for 12 h, incubated with goat anti-rabbit IgG (H & L, HRP) with rhodamine conjugate (1:200) at 25°C for 1 h in darkness, counterstained with 0.5% (w/v) DAPI in 0.1% (v/v) Triton-X-100 at 25°C for 3 min in darkness, and incubated with aqueous mounting medium. .. The fluorescent staining was visualized using a confocal laser scanning microscope (LSM 510; Zeiss, Oberkochen, Germany) at excitation of 350 nm (blue), 490 nm (green), and 540 nm (red) and emission of 475 nm (blue), 520 nm (green), and 565 nm (red).

    Article Title: Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin
    Article Snippet: The ability of QU to cross the BBB, P BBB,QU , was defined as ([ability of QU to cross the membrane with HBMEC/HA]−1 − [ability of QU to cross the membrane without cells]−1 )−1 . .. HBMECs at a density of 1×105 cells/cm2 were seeded on a microscope cover glass in a 24-well plate, cultured in a humidified CO2 incubator for 8 h, treated with 0.025% (w/v) RMP-7-Lf-QU-LS, and cultured in a humidified CO2 incubator for 3 h. In preparing the staining sample, cultured HBMECs were fixed with 10% (v/v) formalin at 25°C for 10 min, treated with 0.5% (v/v) Triton-X-100 at 25°C for 10 min, and incubated with serum blocking solution at 25°C for 30 min. .. The sample was reacted with 1 μg/mL of anti-LfR monoclonal antibody and/or with anti-B2 bradykinin receptor antibody (1:1,000) at 4°C for 12 h, stained with goat anti-mouse polyclonal secondary antibody IgG (H + L) conjugated with rhodamine (1:100) at 25°C for 1 h in darkness, treated with 0.5% (w/v) DAPI in 0.1% (v/v) Triton-X-100 at 25°C for 3 min in darkness, and incubated in an aqueous mounting medium.

    Article Title: Effect of Initial Seeding Density on Human Umbilical Cord Mesenchymal Stromal Cells for Fibrocartilage Tissue Engineering
    Article Snippet: The remaining segments were moved to new centrifuge tubes containing 0.1% trypsin/ethylenediaminetetraacetic acid for another 30 min of incubation at 37°C and were squeezed again to release additional cells from Wharton's jelly. .. Cells in the well plates were recorded as passage 0 (P0) and were fed every 2 to 3 days and maintained in a cell culture incubator (NuAire, Autoflow, 5% carbon dioxide, 37°C, and 90% humidity).

    Expressing:

    Article Title: Identification of Oxygen-Sensitive Transcriptional Programs in Human Embryonic Stem Cells
    Article Snippet: Thereafter, cells were cultured on a substratum of 1:30 dilutions of Matrigel™ (BD Biosciences, San Diego, CA, USA) in medium conditioned by MEF [ , ]. .. To identify differences in global gene expression between hESCs cultured under 20% and 4% O2 , hESCs were passaged into six-well culture dishes and placed in either a standard humidified tissue culture CO2 incubator, (NuAire, IR Autoflow) set at 5% CO2 in air, or a tri-gas incubator (Heraeus HERAcell 150; Kendro Laboratory Products GmbH, Langenselbold, Germany) at a 4% O2 , 5% CO2 setting. .. Oxygen levels, measured by using a Bacharach CO2 /O2 Analyzer (Bacharach, Model 2830, New Kensington, PA, USA), were ∼19% in the standard incubator and 3.5–4.5% in the low O2 incubator.

    Modification:

    Article Title: Effects of the donor age on proliferation, senescence and osteogenic capacity of human urine-derived stem cells
    Article Snippet: The cells were cultured at 37 °C in a 5% CO2 humidified incubator (NU-5500E, Nuaire, Plymouth, MN, USA). .. The cells were cultured at 37 °C in a 5% CO2 humidified incubator (NU-5500E, Nuaire, Plymouth, MN, USA).

    Article Title: Retention of poly(N-isopropylacrylamide) on 3-aminopropyltriethoxysilane
    Article Snippet: The cell culture medium used was Dulbecco's modified eagle's medium + 10% fetal bovine serum + 1% of antibiotic antimycotic solution (100×) and were obtained from Sigma-Aldrich. .. Basic equipment used for this study included a spin coater (p-6000 Spin Coater, Specialty Coating System Inc., Indianapolis, IN), a UV/ozone cleaner (model 42, Jelight), analytical balances with an accuracy of 0.1 mg, a vacuum oven (VWR) and its pump (Welch), a humid CO2 cell incubator (Model NU-5510, NuAire), a contact angle goniometer (Ramé-Hart Instrument Co., Netcong, NJ with a CCD camera attached), an ellipsometer (Rudolph Instruments, Inc., Fairfield, NJ equipped with a λ = 632.8 nm laser), a microscope heating stage (TP-110R, TOKAI HIT), a digital camera, an optical microscope with an eye-piece digital camera, a humidifier, and XPS (PHI VersaProbe II Scanning XPS microprobe) with a monochromatic Al Kα excitation source (x-ray setting: 100 μ m, 25 W and 15 kV; Analyzer mode: FAT).

    Article Title: Aptamer-guided gene targeting in yeast and human cells
    Article Snippet: Human embryonic kidney (HEK-293) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) (Mediatech, Manassas, VA, USA), supplemented with 10% heat-inactivated fetal bovine serum (Gemini Bio-Products, West Sacramento, CA, USA) and 1× penicillin/streptomycin (Lonza, Walkersville, MD, USA). .. Cells were grown at 37°C in a water-jacketed 5% CO2 humidified incubator (NuAire, Plymouth, MN, USA).

    Article Title: Effect of Initial Seeding Density on Human Umbilical Cord Mesenchymal Stromal Cells for Fibrocartilage Tissue Engineering
    Article Snippet: The medium was composed of low-glucose Dulbecco's modified Eagle medium low-glucose Dulbecco's modified Eagle medium (DMEM; Catalog# 11885-092; Invitrogen) and MCDB-201 medium (Catalog# 045k8310; Sigma) supplemented with 1× insulin-transferrin-selenium (Catalog# 51300-044; Invitrogen), 0.15% lipid-rich bovine serum albumin (Catalog# 11020-021; Albumax, Invitrogen), 0.1 nM dexamethasone (Catalog# D2915; Sigma), 10 μM ascorbic acid-2-phosphate (Catalog# A-8960; Sigma), 1× penicillin/streptomycin (Catalog# 30-001-CI; Fisher Scientific, Pittsburgh, PA), 2% fetal bovine serum (FBS; Catalog# 16141079; Invitrogen), 10 ng/mL recombinant human epidermal growth factor (Catalog# 13247-051; Invitrogen), and 10 ng/mL human platelet-derived growth factor BB (Catalog# 220-BB-050; R & D Systems, Minneapolis, MN). .. Cells in the well plates were recorded as passage 0 (P0) and were fed every 2 to 3 days and maintained in a cell culture incubator (NuAire, Autoflow, 5% carbon dioxide, 37°C, and 90% humidity).

    Derivative Assay:

    Article Title: Gold-gold sulfide nanoshell as a novel intensifier for anti-tumor effects of radiofrequency fields
    Article Snippet: CT26 cell line (Pasteur Inst., Iran) derived from colon carcinoma of a BALB/c mouse was grown in RPMI-1640 (Gibco, Life Technologies Corp., USA) supplemented with 10% (v/v) fetal bovine serum (FBS), 100 units/ml penicillin and 100 μg/ml streptomycin. .. Cell culture was performed at 37°C in a 5% CO2 humidified incubator (NU 8500, NuAire Corp., USA).

    High Performance Liquid Chromatography:

    Article Title: Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin
    Article Snippet: The ability of PI to cross the BBB, P BBB,PI , was defined as ([ability of PI to cross the membrane with HBMEC/HA]−1 − [ability of PI to cross the membrane without cells]−1 )−1 . .. A total of 0.025% (w/v) RMP-7-Lf-QU-LS was added to the donor chamber and cultured in a humidified CO2 incubator for 5 h. In all, 20 μL of the liquid in the receiver chamber was sampled every 2.5 h, treated with methanol at 37°C for 30 min, and analyzed using the HPLC-UV system (at 370 nm) described in the “Preparation of QU-LS” section. .. A total of 20 μL of the fresh medium was added to the receiver chamber immediately.

    Flow Cytometry:

    Article Title: Isolation of Exosomes from Semen for in vitro Uptake and HIV-1 Infection Assays
    Article Snippet: This protocol lays the groundwork for evaluating other functional activities of semen exosomes, and for evaluating exosomes from other sources. .. Pipettes (any brand) Sterile tweezers (any brand) Centrifuge (Eppendorf, model: 5415 D) Ultracentrifuge (Beckman Coulter, model: Optima L-90K) SW40Ti rotor (Beckman Coulter, catalog number: 331362) Polyallomer centrifuge tubes 14 × 89 mm for SW41Ti rotor (Beckman Coulter, catalog number: 344059) 37 °C, 5% CO2 cell culture incubator (NuAire, model: NU-5510) FACSCalibur flow cytometer (BD) (see Notes for laser and filter specifications) FACSVerse flow cytometer (BD) (see Notes for laser and filter specifications) FACSAria flow cytometer (BD) (see Notes for laser and filter specifications) Laser scanning confocal microscope (Nikon Instruments, model: Eclipse TE2000) Magnetic separator for 1.7 ml tubes Rotating mixer Microplate reader (Tecan Trading, model: Infinite® M200 Pro) DynaPro Nanostar (Wyatt Technologies, model: DynaPro Nanostar) NanoSight LM10 (Malvern Instruments, model: NanoSight LM10) NanoDrop spectrophotometer (Thermo Fisher Scientific) SW60 Ti rotor (Beckman Coulter, catalog number: 335649) Polyallomer centrifuge tubes 11 × 60 mm for SW60Ti rotor (Beckman Coulter, catalog number: 355636) 7500 fast real-time PCR system (Thermo Fisher Scientific, model: 7500 Fast Real-time PCR System) Luminometer (BioTek Instruments, model: Synergy H1 Hybrid Reader) Laminar flow hood SW32 Ti rotor (Beckman Coulter, model: 369650) Polyallomer centrifuge tubes 25 × 89 mm for SW32Ti rotor (Beckman Coulter, catalog number: 344058) pH meter (any brand) Gel running tank(Thermo Fisher Scientific, Novex(x22122), model: XCell SureLock ® Mini-Cell, catalog number: EI0001) Sephacryl S300-HR 16/60 gel filtration prepacked column (GE Healthcare catalog number: 17-1167-01) BioLogic DuoFlow Workstation (Bio-Rad Laboratories) BioLogic BioFrac fraction collector (Bio-Rad Laboratories) Gel electrophoresis horizontal apparatus (Bio-Rad Laboratories, model: Wide Mini-Sub Cell GT Cell, catalog number: 1704468EDU) Dry Block Heater for microcentrifuge tubes (Thermo Fisher Scientific) .. FlowJo analysis software (TreeStar) BioLogic DuoFlow software version 5.3 (Bio-Rad Laboratories) Dynamics software (Wyatt Technology) NTA software (Malvern Instruments)

    Article Title: Defining human dendritic cell progenitors by multiparametric flow cytometry
    Article Snippet: BD CompBeads set anti-mouse Igκ (BD Biosciences, cat. no. 552843) BD CompBeads set anti-rat Igκ (BD Biosciences, cat. no. 552844) Amine-reactive compensation bead kit (Life Technologies, cat. no. ) .. Biological safety level 2 (BSL-2) tissue culture facilities BSL-2 sorting facilities Class II biological safety cabinet (The Baker Company, cat. no. SG403) Cell incubator (37 °C, 5% CO2 ; Nuaire, cat. no. NU-5500) Tabletop centrifuge with rotors and adapters for 50- and 15-ml conical tubes and 96-well plates (Sorvall, cat. no. 75-004-367) Microcentrifuge (Eppendorf, cat. no. 5424) Inverted microscope (Nikon, cat. no. Eclipse TS100) Hemocytometer (Fisher Scientific, cat. no. 02-671-5) or other cell counter Cryo 1 °C freezing container (Nalgene, cat. no. 5100-0001) LN2 storage systems (Thermo Scientific, cat. no. 7404) BD LSR II flow cytometer running DIVA 8.0, or equivalent (BD Biosciences, special order). .. Our LSR II is equipped with four lasers with a configuration allowing 16-color, 18-parameter flow cytometry ( ) BD Aria III cell sorter running DIVA 8.0, or equivalent (BD Biosciences, special order).

    Article Title: Differing mechanisms of leukocyte recruitment and sensitivity to conditioning by shear stress for endothelial cells treated with tumour necrosis factor-α or interleukin-1β
    Article Snippet: Paragraph title: Culture of endothelial cells under static or flow conditions ... The dish contained culture medium and was placed in a humidified CO2 incubator (Nuaire DH; Triple Red, Thame, Oxfordshire, U.K.).

    Article Title: Molecular Targeted Viral Nanoparticles as Tools for Imaging Cancer
    Article Snippet: Humidified microbiological CO2 incubator (37 °C and 5 % CO2 ) (Nuaire, Plymouth, Manhattan). .. Humidified microbiological CO2 incubator (37 °C and 5 % CO2 ) (Nuaire, Plymouth, Manhattan).

    Cell Culture:

    Article Title: Isolation of Exosomes from Semen for in vitro Uptake and HIV-1 Infection Assays
    Article Snippet: This protocol lays the groundwork for evaluating other functional activities of semen exosomes, and for evaluating exosomes from other sources. .. Pipettes (any brand) Sterile tweezers (any brand) Centrifuge (Eppendorf, model: 5415 D) Ultracentrifuge (Beckman Coulter, model: Optima L-90K) SW40Ti rotor (Beckman Coulter, catalog number: 331362) Polyallomer centrifuge tubes 14 × 89 mm for SW41Ti rotor (Beckman Coulter, catalog number: 344059) 37 °C, 5% CO2 cell culture incubator (NuAire, model: NU-5510) FACSCalibur flow cytometer (BD) (see Notes for laser and filter specifications) FACSVerse flow cytometer (BD) (see Notes for laser and filter specifications) FACSAria flow cytometer (BD) (see Notes for laser and filter specifications) Laser scanning confocal microscope (Nikon Instruments, model: Eclipse TE2000) Magnetic separator for 1.7 ml tubes Rotating mixer Microplate reader (Tecan Trading, model: Infinite® M200 Pro) DynaPro Nanostar (Wyatt Technologies, model: DynaPro Nanostar) NanoSight LM10 (Malvern Instruments, model: NanoSight LM10) NanoDrop spectrophotometer (Thermo Fisher Scientific) SW60 Ti rotor (Beckman Coulter, catalog number: 335649) Polyallomer centrifuge tubes 11 × 60 mm for SW60Ti rotor (Beckman Coulter, catalog number: 355636) 7500 fast real-time PCR system (Thermo Fisher Scientific, model: 7500 Fast Real-time PCR System) Luminometer (BioTek Instruments, model: Synergy H1 Hybrid Reader) Laminar flow hood SW32 Ti rotor (Beckman Coulter, model: 369650) Polyallomer centrifuge tubes 25 × 89 mm for SW32Ti rotor (Beckman Coulter, catalog number: 344058) pH meter (any brand) Gel running tank(Thermo Fisher Scientific, Novex(x22122), model: XCell SureLock ® Mini-Cell, catalog number: EI0001) Sephacryl S300-HR 16/60 gel filtration prepacked column (GE Healthcare catalog number: 17-1167-01) BioLogic DuoFlow Workstation (Bio-Rad Laboratories) BioLogic BioFrac fraction collector (Bio-Rad Laboratories) Gel electrophoresis horizontal apparatus (Bio-Rad Laboratories, model: Wide Mini-Sub Cell GT Cell, catalog number: 1704468EDU) Dry Block Heater for microcentrifuge tubes (Thermo Fisher Scientific) .. FlowJo analysis software (TreeStar) BioLogic DuoFlow software version 5.3 (Bio-Rad Laboratories) Dynamics software (Wyatt Technology) NTA software (Malvern Instruments)

    Article Title: Identification of Oxygen-Sensitive Transcriptional Programs in Human Embryonic Stem Cells
    Article Snippet: Thereafter, cells were cultured on a substratum of 1:30 dilutions of Matrigel™ (BD Biosciences, San Diego, CA, USA) in medium conditioned by MEF [ , ]. .. To identify differences in global gene expression between hESCs cultured under 20% and 4% O2 , hESCs were passaged into six-well culture dishes and placed in either a standard humidified tissue culture CO2 incubator, (NuAire, IR Autoflow) set at 5% CO2 in air, or a tri-gas incubator (Heraeus HERAcell 150; Kendro Laboratory Products GmbH, Langenselbold, Germany) at a 4% O2 , 5% CO2 setting. .. Oxygen levels, measured by using a Bacharach CO2 /O2 Analyzer (Bacharach, Model 2830, New Kensington, PA, USA), were ∼19% in the standard incubator and 3.5–4.5% in the low O2 incubator.

    Article Title: Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment
    Article Snippet: The quantity of the specific protein band in the lanes was analyzed using the ImageJ (version 1.50; National Institutes of Health, Bethesda, MD, USA) program. .. SK-N-MC cells were seeded at a density of 1×104 cells/well in a 24-well flat-bottom tissue culture microtiter plate pretreated with gelatin for 12 h; then, 10 µM of fibrillary Aβ1–42 and fluorescent LIP carriers were added, and the SK-N-MC cells were cultured in a humidified CO2 incubator for 24 h. The culture of SK-N-MC cells was fixed with 0.5 mL of 10% (v/v) formalin at 25°C for 5 min, immersed in 0.5% (v/v) Triton-X-100 at 25°C for 10 min, treated with 200 µL of CAS block at 25°C for 10 min, reacted with antiphospho-protein antibody (anti-p-p38, 1:800; anti-p-JNK, 1:200; and anti-p-ERK5, 1:200) at 4°C for 12 h, incubated with goat anti-rabbit IgG (H & L, HRP) with rhodamine conjugate (1:200) at 25°C for 1 h in darkness, counterstained with 0.5% (w/v) DAPI in 0.1% (v/v) Triton-X-100 at 25°C for 3 min in darkness, and incubated with aqueous mounting medium. .. The fluorescent staining was visualized using a confocal laser scanning microscope (LSM 510; Zeiss, Oberkochen, Germany) at excitation of 350 nm (blue), 490 nm (green), and 540 nm (red) and emission of 475 nm (blue), 520 nm (green), and 565 nm (red).

    Article Title: Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin
    Article Snippet: The ability of PI to cross the BBB, P BBB,PI , was defined as ([ability of PI to cross the membrane with HBMEC/HA]−1 − [ability of PI to cross the membrane without cells]−1 )−1 . .. A total of 0.025% (w/v) RMP-7-Lf-QU-LS was added to the donor chamber and cultured in a humidified CO2 incubator for 5 h. In all, 20 μL of the liquid in the receiver chamber was sampled every 2.5 h, treated with methanol at 37°C for 30 min, and analyzed using the HPLC-UV system (at 370 nm) described in the “Preparation of QU-LS” section. .. A total of 20 μL of the fresh medium was added to the receiver chamber immediately.

    Article Title: In vitro Flow Adhesion Assay for Analyzing Shear-resistant Adhesion of Metastatic Cancer Cells to Endothelial Cells
    Article Snippet: Fibronectin from human plasma (Sigma-Aldrich, catalog number: F0895) Cell culture medium for HMVEC (see Recipes) Cell culture medium for Cancer cells (see Recipes) .. Glass beaker (1,000 ml) Stir bar 37 °C, 5% CO2 cell culture incubator (NuAire, model: In-VitroCell ES NU-5800) Cell culture centrifuge (Eppendorf, model: 5702) Inverted Microscope with camera (Nikon, model: Eclipse TS100) Camera (McCrone, model: MicroPublisher 3.3 RTV) KDS Legato™ 200 syringe pump (KD Scientific) Ibidi Heating System [Ibidi Temperature Controller ©ibidimodel (ibidi, model: Version 3.3)] Magnetic hot stir (Thermo Fisher Scientific, catalog number: 200-48SH) Thermometer (Thermo Fisher Scientific, catalog number: 13-201-695) Level (Stanley Black & Decker, catalog number: 43-511) .. Q-capture pro 7.0 (Qimaging)

    Article Title: Immunomodulatory effect of an isolated fraction from Tinospora crispa on intracellular expression of INF-γ, IL-6 and IL-8
    Article Snippet: A murine macrophage cell line RAW264.7 obtained from American Type Culture Collection (ATCC, Rockville, MD) was cultured in DMEM supplemented with 4500 g of glucose/L, 110 mg of sodium pyruvate/L, 1% penicillin–streptomycin (Sigma-Aldrich, UK), and 10% heat-inactivated fetal bovine serum (FBS) (J R Scientific, Inc., USA). .. The cells were cultured at 37°C in a 5% CO2 atmosphere incubator (NuAire, Plymouth, MN, USA). .. The cells were subcultured when at 80% confluence, and the cell suspension was diluted to 5 × 105 cells/mL for the experiments [ ].

    Article Title: Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin
    Article Snippet: The ability of QU to cross the BBB, P BBB,QU , was defined as ([ability of QU to cross the membrane with HBMEC/HA]−1 − [ability of QU to cross the membrane without cells]−1 )−1 . .. HBMECs at a density of 1×105 cells/cm2 were seeded on a microscope cover glass in a 24-well plate, cultured in a humidified CO2 incubator for 8 h, treated with 0.025% (w/v) RMP-7-Lf-QU-LS, and cultured in a humidified CO2 incubator for 3 h. In preparing the staining sample, cultured HBMECs were fixed with 10% (v/v) formalin at 25°C for 10 min, treated with 0.5% (v/v) Triton-X-100 at 25°C for 10 min, and incubated with serum blocking solution at 25°C for 30 min. .. The sample was reacted with 1 μg/mL of anti-LfR monoclonal antibody and/or with anti-B2 bradykinin receptor antibody (1:1,000) at 4°C for 12 h, stained with goat anti-mouse polyclonal secondary antibody IgG (H + L) conjugated with rhodamine (1:100) at 25°C for 1 h in darkness, treated with 0.5% (w/v) DAPI in 0.1% (v/v) Triton-X-100 at 25°C for 3 min in darkness, and incubated in an aqueous mounting medium.

    Article Title: Retention of poly(N-isopropylacrylamide) on 3-aminopropyltriethoxysilane
    Article Snippet: The cell culture medium used was Dulbecco's modified eagle's medium + 10% fetal bovine serum + 1% of antibiotic antimycotic solution (100×) and were obtained from Sigma-Aldrich. .. Basic equipment used for this study included a spin coater (p-6000 Spin Coater, Specialty Coating System Inc., Indianapolis, IN), a UV/ozone cleaner (model 42, Jelight), analytical balances with an accuracy of 0.1 mg, a vacuum oven (VWR) and its pump (Welch), a humid CO2 cell incubator (Model NU-5510, NuAire), a contact angle goniometer (Ramé-Hart Instrument Co., Netcong, NJ with a CCD camera attached), an ellipsometer (Rudolph Instruments, Inc., Fairfield, NJ equipped with a λ = 632.8 nm laser), a microscope heating stage (TP-110R, TOKAI HIT), a digital camera, an optical microscope with an eye-piece digital camera, a humidifier, and XPS (PHI VersaProbe II Scanning XPS microprobe) with a monochromatic Al Kα excitation source (x-ray setting: 100 μ m, 25 W and 15 kV; Analyzer mode: FAT).

    Article Title: Analysis of proteome response to the mobile phone radiation in two types of human primary endothelial cells
    Article Snippet: The sXc-1800 exposure system, developed and provided by the IT'IS Foundation (Zurich, Switzerland) was used for exposing cells to 1800 MHz GSM signal (Figure ). .. Briefly: The system consists of two identical exposure chambers mounted inside the same cell culture incubator (NuAire US Autoflow CO2 Water-Jacketed Incubator, NuAire, USA). .. One of the chambers acted as a sham control (no radiation) and the other as an experimental (with radiation).

    Article Title: Neferine ameliorates cardiomyoblast apoptosis induced by doxorubicin: possible role in modulating NADPH oxidase/ROS-mediated NFκB redox signaling cascade
    Article Snippet: Cells were cultured in DMEM with 10% FBS containing antibiotics (penicillin and streptomycin) in 75 cm2 flask. .. Cell culture flask was maintained at 37 °C in a humidified CO2 incubator (Nuaire make) with 5% CO2 supply. .. Cells were sub-cultured at 80% confluency and media was changed once in two days.

    Article Title: Gold-gold sulfide nanoshell as a novel intensifier for anti-tumor effects of radiofrequency fields
    Article Snippet: CT26 cell line (Pasteur Inst., Iran) derived from colon carcinoma of a BALB/c mouse was grown in RPMI-1640 (Gibco, Life Technologies Corp., USA) supplemented with 10% (v/v) fetal bovine serum (FBS), 100 units/ml penicillin and 100 μg/ml streptomycin. .. Cell culture was performed at 37°C in a 5% CO2 humidified incubator (NU 8500, NuAire Corp., USA). .. The cells covered the bottom of the flask as a monolayer after 2-3 days of cell growth and proliferation.

    Article Title: Effect of Initial Seeding Density on Human Umbilical Cord Mesenchymal Stromal Cells for Fibrocartilage Tissue Engineering
    Article Snippet: The medium was composed of low-glucose Dulbecco's modified Eagle medium low-glucose Dulbecco's modified Eagle medium (DMEM; Catalog# 11885-092; Invitrogen) and MCDB-201 medium (Catalog# 045k8310; Sigma) supplemented with 1× insulin-transferrin-selenium (Catalog# 51300-044; Invitrogen), 0.15% lipid-rich bovine serum albumin (Catalog# 11020-021; Albumax, Invitrogen), 0.1 nM dexamethasone (Catalog# D2915; Sigma), 10 μM ascorbic acid-2-phosphate (Catalog# A-8960; Sigma), 1× penicillin/streptomycin (Catalog# 30-001-CI; Fisher Scientific, Pittsburgh, PA), 2% fetal bovine serum (FBS; Catalog# 16141079; Invitrogen), 10 ng/mL recombinant human epidermal growth factor (Catalog# 13247-051; Invitrogen), and 10 ng/mL human platelet-derived growth factor BB (Catalog# 220-BB-050; R & D Systems, Minneapolis, MN). .. Cells in the well plates were recorded as passage 0 (P0) and were fed every 2 to 3 days and maintained in a cell culture incubator (NuAire, Autoflow, 5% carbon dioxide, 37°C, and 90% humidity). .. When cells had reached 80% to 90% confluence, they were detached and plated into 25-cm2 flasks.

    Generated:

    Article Title: Analysis of proteome response to the mobile phone radiation in two types of human primary endothelial cells
    Article Snippet: Briefly: The system consists of two identical exposure chambers mounted inside the same cell culture incubator (NuAire US Autoflow CO2 Water-Jacketed Incubator, NuAire, USA). .. Briefly: The system consists of two identical exposure chambers mounted inside the same cell culture incubator (NuAire US Autoflow CO2 Water-Jacketed Incubator, NuAire, USA).

    other:

    Article Title: Effects of hypoxia on the proliferation, mineralization and ultrastructure of human periodontal ligament fibroblasts in vitro
    Article Snippet: HPLFs in the three hypoxia groups were cultivated in a three-gas (CO2 /O2 /N2 ) incubator (NuAire Inc., Plymouth, MN, USA), and the HPLFs in the control group were cultivated in a common CO2 incubator (NuAire, Inc., Plymouth, MN, USA).

    Inverted Microscopy:

    Article Title: Defining human dendritic cell progenitors by multiparametric flow cytometry
    Article Snippet: BD CompBeads set anti-mouse Igκ (BD Biosciences, cat. no. 552843) BD CompBeads set anti-rat Igκ (BD Biosciences, cat. no. 552844) Amine-reactive compensation bead kit (Life Technologies, cat. no. ) .. Biological safety level 2 (BSL-2) tissue culture facilities BSL-2 sorting facilities Class II biological safety cabinet (The Baker Company, cat. no. SG403) Cell incubator (37 °C, 5% CO2 ; Nuaire, cat. no. NU-5500) Tabletop centrifuge with rotors and adapters for 50- and 15-ml conical tubes and 96-well plates (Sorvall, cat. no. 75-004-367) Microcentrifuge (Eppendorf, cat. no. 5424) Inverted microscope (Nikon, cat. no. Eclipse TS100) Hemocytometer (Fisher Scientific, cat. no. 02-671-5) or other cell counter Cryo 1 °C freezing container (Nalgene, cat. no. 5100-0001) LN2 storage systems (Thermo Scientific, cat. no. 7404) BD LSR II flow cytometer running DIVA 8.0, or equivalent (BD Biosciences, special order). .. Our LSR II is equipped with four lasers with a configuration allowing 16-color, 18-parameter flow cytometry ( ) BD Aria III cell sorter running DIVA 8.0, or equivalent (BD Biosciences, special order).

    Article Title: In vitro Flow Adhesion Assay for Analyzing Shear-resistant Adhesion of Metastatic Cancer Cells to Endothelial Cells
    Article Snippet: Fibronectin from human plasma (Sigma-Aldrich, catalog number: F0895) Cell culture medium for HMVEC (see Recipes) Cell culture medium for Cancer cells (see Recipes) .. Glass beaker (1,000 ml) Stir bar 37 °C, 5% CO2 cell culture incubator (NuAire, model: In-VitroCell ES NU-5800) Cell culture centrifuge (Eppendorf, model: 5702) Inverted Microscope with camera (Nikon, model: Eclipse TS100) Camera (McCrone, model: MicroPublisher 3.3 RTV) KDS Legato™ 200 syringe pump (KD Scientific) Ibidi Heating System [Ibidi Temperature Controller ©ibidimodel (ibidi, model: Version 3.3)] Magnetic hot stir (Thermo Fisher Scientific, catalog number: 200-48SH) Thermometer (Thermo Fisher Scientific, catalog number: 13-201-695) Level (Stanley Black & Decker, catalog number: 43-511) .. Q-capture pro 7.0 (Qimaging)

    Sequencing:

    Article Title: Hypoxia-regulated human periodontal ligament cells via Wnt/β-catenin signaling pathway
    Article Snippet: The 1st, 2nd, and 3rd antibodies were added in sequence. .. According to various hypoxic conditions, hPDLCs were assigned into 3 groups randomly as follows: slight hypoxia group, 5% O2 content; severe hypoxia group, 1% O2 content; and the control group, 21% O2 content. hPDLCs in the 2 hypoxia groups were cultivated in a 3-gas (CO2 /O2 /N2 ) incubator (NuAire Inc., Plymouth, MN), and the hPDLCs in the control group were cultivated in a common CO2 incubator (NuAire, Inc.).

    Binding Assay:

    Article Title: Aptamer-guided gene targeting in yeast and human cells
    Article Snippet: Cells were grown at 37°C in a water-jacketed 5% CO2 humidified incubator (NuAire, Plymouth, MN, USA). .. Plasmid pEGFP300-disDsRed2 was modified to make plasmid pLDSLm. pEGFP300-disDsRed2 is identical to pdisEGFP300-disDsRed2 described in Katz et al. ( ) with the disrupted non-functional GFP replaced by a functional GFP gene.

    Nucleic Acid Electrophoresis:

    Article Title: Isolation of Exosomes from Semen for in vitro Uptake and HIV-1 Infection Assays
    Article Snippet: This protocol lays the groundwork for evaluating other functional activities of semen exosomes, and for evaluating exosomes from other sources. .. Pipettes (any brand) Sterile tweezers (any brand) Centrifuge (Eppendorf, model: 5415 D) Ultracentrifuge (Beckman Coulter, model: Optima L-90K) SW40Ti rotor (Beckman Coulter, catalog number: 331362) Polyallomer centrifuge tubes 14 × 89 mm for SW41Ti rotor (Beckman Coulter, catalog number: 344059) 37 °C, 5% CO2 cell culture incubator (NuAire, model: NU-5510) FACSCalibur flow cytometer (BD) (see Notes for laser and filter specifications) FACSVerse flow cytometer (BD) (see Notes for laser and filter specifications) FACSAria flow cytometer (BD) (see Notes for laser and filter specifications) Laser scanning confocal microscope (Nikon Instruments, model: Eclipse TE2000) Magnetic separator for 1.7 ml tubes Rotating mixer Microplate reader (Tecan Trading, model: Infinite® M200 Pro) DynaPro Nanostar (Wyatt Technologies, model: DynaPro Nanostar) NanoSight LM10 (Malvern Instruments, model: NanoSight LM10) NanoDrop spectrophotometer (Thermo Fisher Scientific) SW60 Ti rotor (Beckman Coulter, catalog number: 335649) Polyallomer centrifuge tubes 11 × 60 mm for SW60Ti rotor (Beckman Coulter, catalog number: 355636) 7500 fast real-time PCR system (Thermo Fisher Scientific, model: 7500 Fast Real-time PCR System) Luminometer (BioTek Instruments, model: Synergy H1 Hybrid Reader) Laminar flow hood SW32 Ti rotor (Beckman Coulter, model: 369650) Polyallomer centrifuge tubes 25 × 89 mm for SW32Ti rotor (Beckman Coulter, catalog number: 344058) pH meter (any brand) Gel running tank(Thermo Fisher Scientific, Novex(x22122), model: XCell SureLock ® Mini-Cell, catalog number: EI0001) Sephacryl S300-HR 16/60 gel filtration prepacked column (GE Healthcare catalog number: 17-1167-01) BioLogic DuoFlow Workstation (Bio-Rad Laboratories) BioLogic BioFrac fraction collector (Bio-Rad Laboratories) Gel electrophoresis horizontal apparatus (Bio-Rad Laboratories, model: Wide Mini-Sub Cell GT Cell, catalog number: 1704468EDU) Dry Block Heater for microcentrifuge tubes (Thermo Fisher Scientific) .. FlowJo analysis software (TreeStar) BioLogic DuoFlow software version 5.3 (Bio-Rad Laboratories) Dynamics software (Wyatt Technology) NTA software (Malvern Instruments)

    Isolation:

    Article Title: Effects of the donor age on proliferation, senescence and osteogenic capacity of human urine-derived stem cells
    Article Snippet: Paragraph title: Isolation and culture of hUSCs in vitro ... The cells were cultured at 37 °C in a 5% CO2 humidified incubator (NU-5500E, Nuaire, Plymouth, MN, USA).

    Immunohistochemistry:

    Article Title: Hypoxia-regulated human periodontal ligament cells via Wnt/β-catenin signaling pathway
    Article Snippet: Paragraph title: Identification of hPDLCs using avidin–biotin complex immunohistochemistry ... According to various hypoxic conditions, hPDLCs were assigned into 3 groups randomly as follows: slight hypoxia group, 5% O2 content; severe hypoxia group, 1% O2 content; and the control group, 21% O2 content. hPDLCs in the 2 hypoxia groups were cultivated in a 3-gas (CO2 /O2 /N2 ) incubator (NuAire Inc., Plymouth, MN), and the hPDLCs in the control group were cultivated in a common CO2 incubator (NuAire, Inc.).

    Microscopy:

    Article Title: Hypoxia-regulated human periodontal ligament cells via Wnt/β-catenin signaling pathway
    Article Snippet: Images were captured under a microscope (Olympus, Tokyo, Japan). .. According to various hypoxic conditions, hPDLCs were assigned into 3 groups randomly as follows: slight hypoxia group, 5% O2 content; severe hypoxia group, 1% O2 content; and the control group, 21% O2 content. hPDLCs in the 2 hypoxia groups were cultivated in a 3-gas (CO2 /O2 /N2 ) incubator (NuAire Inc., Plymouth, MN), and the hPDLCs in the control group were cultivated in a common CO2 incubator (NuAire, Inc.).

    Article Title: Isolation of Exosomes from Semen for in vitro Uptake and HIV-1 Infection Assays
    Article Snippet: This protocol lays the groundwork for evaluating other functional activities of semen exosomes, and for evaluating exosomes from other sources. .. Pipettes (any brand) Sterile tweezers (any brand) Centrifuge (Eppendorf, model: 5415 D) Ultracentrifuge (Beckman Coulter, model: Optima L-90K) SW40Ti rotor (Beckman Coulter, catalog number: 331362) Polyallomer centrifuge tubes 14 × 89 mm for SW41Ti rotor (Beckman Coulter, catalog number: 344059) 37 °C, 5% CO2 cell culture incubator (NuAire, model: NU-5510) FACSCalibur flow cytometer (BD) (see Notes for laser and filter specifications) FACSVerse flow cytometer (BD) (see Notes for laser and filter specifications) FACSAria flow cytometer (BD) (see Notes for laser and filter specifications) Laser scanning confocal microscope (Nikon Instruments, model: Eclipse TE2000) Magnetic separator for 1.7 ml tubes Rotating mixer Microplate reader (Tecan Trading, model: Infinite® M200 Pro) DynaPro Nanostar (Wyatt Technologies, model: DynaPro Nanostar) NanoSight LM10 (Malvern Instruments, model: NanoSight LM10) NanoDrop spectrophotometer (Thermo Fisher Scientific) SW60 Ti rotor (Beckman Coulter, catalog number: 335649) Polyallomer centrifuge tubes 11 × 60 mm for SW60Ti rotor (Beckman Coulter, catalog number: 355636) 7500 fast real-time PCR system (Thermo Fisher Scientific, model: 7500 Fast Real-time PCR System) Luminometer (BioTek Instruments, model: Synergy H1 Hybrid Reader) Laminar flow hood SW32 Ti rotor (Beckman Coulter, model: 369650) Polyallomer centrifuge tubes 25 × 89 mm for SW32Ti rotor (Beckman Coulter, catalog number: 344058) pH meter (any brand) Gel running tank(Thermo Fisher Scientific, Novex(x22122), model: XCell SureLock ® Mini-Cell, catalog number: EI0001) Sephacryl S300-HR 16/60 gel filtration prepacked column (GE Healthcare catalog number: 17-1167-01) BioLogic DuoFlow Workstation (Bio-Rad Laboratories) BioLogic BioFrac fraction collector (Bio-Rad Laboratories) Gel electrophoresis horizontal apparatus (Bio-Rad Laboratories, model: Wide Mini-Sub Cell GT Cell, catalog number: 1704468EDU) Dry Block Heater for microcentrifuge tubes (Thermo Fisher Scientific) .. FlowJo analysis software (TreeStar) BioLogic DuoFlow software version 5.3 (Bio-Rad Laboratories) Dynamics software (Wyatt Technology) NTA software (Malvern Instruments)

    Article Title: Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin
    Article Snippet: The ability of QU to cross the BBB, P BBB,QU , was defined as ([ability of QU to cross the membrane with HBMEC/HA]−1 − [ability of QU to cross the membrane without cells]−1 )−1 . .. HBMECs at a density of 1×105 cells/cm2 were seeded on a microscope cover glass in a 24-well plate, cultured in a humidified CO2 incubator for 8 h, treated with 0.025% (w/v) RMP-7-Lf-QU-LS, and cultured in a humidified CO2 incubator for 3 h. In preparing the staining sample, cultured HBMECs were fixed with 10% (v/v) formalin at 25°C for 10 min, treated with 0.5% (v/v) Triton-X-100 at 25°C for 10 min, and incubated with serum blocking solution at 25°C for 30 min. .. The sample was reacted with 1 μg/mL of anti-LfR monoclonal antibody and/or with anti-B2 bradykinin receptor antibody (1:1,000) at 4°C for 12 h, stained with goat anti-mouse polyclonal secondary antibody IgG (H + L) conjugated with rhodamine (1:100) at 25°C for 1 h in darkness, treated with 0.5% (w/v) DAPI in 0.1% (v/v) Triton-X-100 at 25°C for 3 min in darkness, and incubated in an aqueous mounting medium.

    Article Title: Retention of poly(N-isopropylacrylamide) on 3-aminopropyltriethoxysilane
    Article Snippet: Other supplies included microscope glass slides obtained from VWR, silicon wafers (P type P⟨100⟩) obtained from Silicon specialist, and treated 24-well plates and 35 mm culture dishes, both obtained from greiner bio-one. .. Basic equipment used for this study included a spin coater (p-6000 Spin Coater, Specialty Coating System Inc., Indianapolis, IN), a UV/ozone cleaner (model 42, Jelight), analytical balances with an accuracy of 0.1 mg, a vacuum oven (VWR) and its pump (Welch), a humid CO2 cell incubator (Model NU-5510, NuAire), a contact angle goniometer (Ramé-Hart Instrument Co., Netcong, NJ with a CCD camera attached), an ellipsometer (Rudolph Instruments, Inc., Fairfield, NJ equipped with a λ = 632.8 nm laser), a microscope heating stage (TP-110R, TOKAI HIT), a digital camera, an optical microscope with an eye-piece digital camera, a humidifier, and XPS (PHI VersaProbe II Scanning XPS microprobe) with a monochromatic Al Kα excitation source (x-ray setting: 100 μ m, 25 W and 15 kV; Analyzer mode: FAT). .. A take-off angle of 45° was used.

    Avidin-Biotin Assay:

    Article Title: Hypoxia-regulated human periodontal ligament cells via Wnt/β-catenin signaling pathway
    Article Snippet: Paragraph title: Identification of hPDLCs using avidin–biotin complex immunohistochemistry ... According to various hypoxic conditions, hPDLCs were assigned into 3 groups randomly as follows: slight hypoxia group, 5% O2 content; severe hypoxia group, 1% O2 content; and the control group, 21% O2 content. hPDLCs in the 2 hypoxia groups were cultivated in a 3-gas (CO2 /O2 /N2 ) incubator (NuAire Inc., Plymouth, MN), and the hPDLCs in the control group were cultivated in a common CO2 incubator (NuAire, Inc.).

    Construct:

    Article Title: Differing mechanisms of leukocyte recruitment and sensitivity to conditioning by shear stress for endothelial cells treated with tumour necrosis factor-α or interleukin-1β
    Article Snippet: After seeding with HUVEC for 1 h, microslides were placed into specially constructed glass dishes, and attached to glass tubing which had been fused into the wall. .. The dish contained culture medium and was placed in a humidified CO2 incubator (Nuaire DH; Triple Red, Thame, Oxfordshire, U.K.).

    Plasmid Preparation:

    Article Title: Aptamer-guided gene targeting in yeast and human cells
    Article Snippet: Cells were grown at 37°C in a water-jacketed 5% CO2 humidified incubator (NuAire, Plymouth, MN, USA). .. Plasmid pLDSLm contains the DsRed2 gene, responsible for producing red fluorescent protein (RFP), disrupted by a 37-bp region containing the 18-bp site for the I-SceI endonuclease preceded by two stop codons.

    Software:

    Article Title: Defining human dendritic cell progenitors by multiparametric flow cytometry
    Article Snippet: Biological safety level 2 (BSL-2) tissue culture facilities BSL-2 sorting facilities Class II biological safety cabinet (The Baker Company, cat. no. SG403) Cell incubator (37 °C, 5% CO2 ; Nuaire, cat. no. NU-5500) Tabletop centrifuge with rotors and adapters for 50- and 15-ml conical tubes and 96-well plates (Sorvall, cat. no. 75-004-367) Microcentrifuge (Eppendorf, cat. no. 5424) Inverted microscope (Nikon, cat. no. Eclipse TS100) Hemocytometer (Fisher Scientific, cat. no. 02-671-5) or other cell counter Cryo 1 °C freezing container (Nalgene, cat. no. 5100-0001) LN2 storage systems (Thermo Scientific, cat. no. 7404) BD LSR II flow cytometer running DIVA 8.0, or equivalent (BD Biosciences, special order). .. Our LSR II is equipped with four lasers with a configuration allowing 16-color, 18-parameter flow cytometry ( ) BD Aria III cell sorter running DIVA 8.0, or equivalent (BD Biosciences, special order).

    Functional Assay:

    Article Title: Aptamer-guided gene targeting in yeast and human cells
    Article Snippet: Cells were grown at 37°C in a water-jacketed 5% CO2 humidified incubator (NuAire, Plymouth, MN, USA). .. Plasmid pLDSLm contains the DsRed2 gene, responsible for producing red fluorescent protein (RFP), disrupted by a 37-bp region containing the 18-bp site for the I-SceI endonuclease preceded by two stop codons.

    Recombinant:

    Article Title: Effect of Initial Seeding Density on Human Umbilical Cord Mesenchymal Stromal Cells for Fibrocartilage Tissue Engineering
    Article Snippet: The medium was composed of low-glucose Dulbecco's modified Eagle medium low-glucose Dulbecco's modified Eagle medium (DMEM; Catalog# 11885-092; Invitrogen) and MCDB-201 medium (Catalog# 045k8310; Sigma) supplemented with 1× insulin-transferrin-selenium (Catalog# 51300-044; Invitrogen), 0.15% lipid-rich bovine serum albumin (Catalog# 11020-021; Albumax, Invitrogen), 0.1 nM dexamethasone (Catalog# D2915; Sigma), 10 μM ascorbic acid-2-phosphate (Catalog# A-8960; Sigma), 1× penicillin/streptomycin (Catalog# 30-001-CI; Fisher Scientific, Pittsburgh, PA), 2% fetal bovine serum (FBS; Catalog# 16141079; Invitrogen), 10 ng/mL recombinant human epidermal growth factor (Catalog# 13247-051; Invitrogen), and 10 ng/mL human platelet-derived growth factor BB (Catalog# 220-BB-050; R & D Systems, Minneapolis, MN). .. Cells in the well plates were recorded as passage 0 (P0) and were fed every 2 to 3 days and maintained in a cell culture incubator (NuAire, Autoflow, 5% carbon dioxide, 37°C, and 90% humidity).

    In Vitro:

    Article Title: Effects of the donor age on proliferation, senescence and osteogenic capacity of human urine-derived stem cells
    Article Snippet: Paragraph title: Isolation and culture of hUSCs in vitro ... The cells were cultured at 37 °C in a 5% CO2 humidified incubator (NU-5500E, Nuaire, Plymouth, MN, USA).

    Spectrophotometry:

    Article Title: Isolation of Exosomes from Semen for in vitro Uptake and HIV-1 Infection Assays
    Article Snippet: This protocol lays the groundwork for evaluating other functional activities of semen exosomes, and for evaluating exosomes from other sources. .. Pipettes (any brand) Sterile tweezers (any brand) Centrifuge (Eppendorf, model: 5415 D) Ultracentrifuge (Beckman Coulter, model: Optima L-90K) SW40Ti rotor (Beckman Coulter, catalog number: 331362) Polyallomer centrifuge tubes 14 × 89 mm for SW41Ti rotor (Beckman Coulter, catalog number: 344059) 37 °C, 5% CO2 cell culture incubator (NuAire, model: NU-5510) FACSCalibur flow cytometer (BD) (see Notes for laser and filter specifications) FACSVerse flow cytometer (BD) (see Notes for laser and filter specifications) FACSAria flow cytometer (BD) (see Notes for laser and filter specifications) Laser scanning confocal microscope (Nikon Instruments, model: Eclipse TE2000) Magnetic separator for 1.7 ml tubes Rotating mixer Microplate reader (Tecan Trading, model: Infinite® M200 Pro) DynaPro Nanostar (Wyatt Technologies, model: DynaPro Nanostar) NanoSight LM10 (Malvern Instruments, model: NanoSight LM10) NanoDrop spectrophotometer (Thermo Fisher Scientific) SW60 Ti rotor (Beckman Coulter, catalog number: 335649) Polyallomer centrifuge tubes 11 × 60 mm for SW60Ti rotor (Beckman Coulter, catalog number: 355636) 7500 fast real-time PCR system (Thermo Fisher Scientific, model: 7500 Fast Real-time PCR System) Luminometer (BioTek Instruments, model: Synergy H1 Hybrid Reader) Laminar flow hood SW32 Ti rotor (Beckman Coulter, model: 369650) Polyallomer centrifuge tubes 25 × 89 mm for SW32Ti rotor (Beckman Coulter, catalog number: 344058) pH meter (any brand) Gel running tank(Thermo Fisher Scientific, Novex(x22122), model: XCell SureLock ® Mini-Cell, catalog number: EI0001) Sephacryl S300-HR 16/60 gel filtration prepacked column (GE Healthcare catalog number: 17-1167-01) BioLogic DuoFlow Workstation (Bio-Rad Laboratories) BioLogic BioFrac fraction collector (Bio-Rad Laboratories) Gel electrophoresis horizontal apparatus (Bio-Rad Laboratories, model: Wide Mini-Sub Cell GT Cell, catalog number: 1704468EDU) Dry Block Heater for microcentrifuge tubes (Thermo Fisher Scientific) .. FlowJo analysis software (TreeStar) BioLogic DuoFlow software version 5.3 (Bio-Rad Laboratories) Dynamics software (Wyatt Technology) NTA software (Malvern Instruments)

    Cell Counting:

    Article Title: Molecular Targeted Viral Nanoparticles as Tools for Imaging Cancer
    Article Snippet: Humidified microbiological CO2 incubator (37 °C and 5 % CO2 ) (Nuaire, Plymouth, Manhattan). .. Humidified microbiological CO2 incubator (37 °C and 5 % CO2 ) (Nuaire, Plymouth, Manhattan).

    Article Title: Gold-gold sulfide nanoshell as a novel intensifier for anti-tumor effects of radiofrequency fields
    Article Snippet: Cell culture was performed at 37°C in a 5% CO2 humidified incubator (NU 8500, NuAire Corp., USA). .. Cell culture was performed at 37°C in a 5% CO2 humidified incubator (NU 8500, NuAire Corp., USA).

    Staining:

    Article Title: Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin
    Article Snippet: The ability of QU to cross the BBB, P BBB,QU , was defined as ([ability of QU to cross the membrane with HBMEC/HA]−1 − [ability of QU to cross the membrane without cells]−1 )−1 . .. HBMECs at a density of 1×105 cells/cm2 were seeded on a microscope cover glass in a 24-well plate, cultured in a humidified CO2 incubator for 8 h, treated with 0.025% (w/v) RMP-7-Lf-QU-LS, and cultured in a humidified CO2 incubator for 3 h. In preparing the staining sample, cultured HBMECs were fixed with 10% (v/v) formalin at 25°C for 10 min, treated with 0.5% (v/v) Triton-X-100 at 25°C for 10 min, and incubated with serum blocking solution at 25°C for 30 min. .. The sample was reacted with 1 μg/mL of anti-LfR monoclonal antibody and/or with anti-B2 bradykinin receptor antibody (1:1,000) at 4°C for 12 h, stained with goat anti-mouse polyclonal secondary antibody IgG (H + L) conjugated with rhodamine (1:100) at 25°C for 1 h in darkness, treated with 0.5% (w/v) DAPI in 0.1% (v/v) Triton-X-100 at 25°C for 3 min in darkness, and incubated in an aqueous mounting medium.

    Article Title: Gold-gold sulfide nanoshell as a novel intensifier for anti-tumor effects of radiofrequency fields
    Article Snippet: Cell culture was performed at 37°C in a 5% CO2 humidified incubator (NU 8500, NuAire Corp., USA). .. Cell culture was performed at 37°C in a 5% CO2 humidified incubator (NU 8500, NuAire Corp., USA).

    Hood:

    Article Title: Isolation of Exosomes from Semen for in vitro Uptake and HIV-1 Infection Assays
    Article Snippet: This protocol lays the groundwork for evaluating other functional activities of semen exosomes, and for evaluating exosomes from other sources. .. Pipettes (any brand) Sterile tweezers (any brand) Centrifuge (Eppendorf, model: 5415 D) Ultracentrifuge (Beckman Coulter, model: Optima L-90K) SW40Ti rotor (Beckman Coulter, catalog number: 331362) Polyallomer centrifuge tubes 14 × 89 mm for SW41Ti rotor (Beckman Coulter, catalog number: 344059) 37 °C, 5% CO2 cell culture incubator (NuAire, model: NU-5510) FACSCalibur flow cytometer (BD) (see Notes for laser and filter specifications) FACSVerse flow cytometer (BD) (see Notes for laser and filter specifications) FACSAria flow cytometer (BD) (see Notes for laser and filter specifications) Laser scanning confocal microscope (Nikon Instruments, model: Eclipse TE2000) Magnetic separator for 1.7 ml tubes Rotating mixer Microplate reader (Tecan Trading, model: Infinite® M200 Pro) DynaPro Nanostar (Wyatt Technologies, model: DynaPro Nanostar) NanoSight LM10 (Malvern Instruments, model: NanoSight LM10) NanoDrop spectrophotometer (Thermo Fisher Scientific) SW60 Ti rotor (Beckman Coulter, catalog number: 335649) Polyallomer centrifuge tubes 11 × 60 mm for SW60Ti rotor (Beckman Coulter, catalog number: 355636) 7500 fast real-time PCR system (Thermo Fisher Scientific, model: 7500 Fast Real-time PCR System) Luminometer (BioTek Instruments, model: Synergy H1 Hybrid Reader) Laminar flow hood SW32 Ti rotor (Beckman Coulter, model: 369650) Polyallomer centrifuge tubes 25 × 89 mm for SW32Ti rotor (Beckman Coulter, catalog number: 344058) pH meter (any brand) Gel running tank(Thermo Fisher Scientific, Novex(x22122), model: XCell SureLock ® Mini-Cell, catalog number: EI0001) Sephacryl S300-HR 16/60 gel filtration prepacked column (GE Healthcare catalog number: 17-1167-01) BioLogic DuoFlow Workstation (Bio-Rad Laboratories) BioLogic BioFrac fraction collector (Bio-Rad Laboratories) Gel electrophoresis horizontal apparatus (Bio-Rad Laboratories, model: Wide Mini-Sub Cell GT Cell, catalog number: 1704468EDU) Dry Block Heater for microcentrifuge tubes (Thermo Fisher Scientific) .. FlowJo analysis software (TreeStar) BioLogic DuoFlow software version 5.3 (Bio-Rad Laboratories) Dynamics software (Wyatt Technology) NTA software (Malvern Instruments)

    Article Title: Molecular Targeted Viral Nanoparticles as Tools for Imaging Cancer
    Article Snippet: Humidified microbiological CO2 incubator (37 °C and 5 % CO2 ) (Nuaire, Plymouth, Manhattan). .. Humidified microbiological CO2 incubator (37 °C and 5 % CO2 ) (Nuaire, Plymouth, Manhattan).

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    NuAire humidified co2 incubator
    Effect of neferine and DOX on the pro-inflammatory transcription factor, NFκB p65 and its downstream regulators, <t>COX2</t> and TNF-α ( A ) Time dependent expression patterns of NFκB (nuclear extract) and COX2 and TNF-α (cytoplasmic extract) (0 h, 1 h, 3 h, 6 h, 12 h and 24 h). ( B ) DOX-induced nuclear translocation of NFκB, expression of COX2 and TNF-α was reduced by neferine treatment. Results were confirmed in the presence of NFκB inhibitor (QNZ). ( C ) Densitometry analysis of the protein bands of NFκB, COX2 and TNF-α. The results shown are mean ± s.d. of three individual experiments. * p
    Humidified Co2 Incubator, supplied by NuAire, used in various techniques. Bioz Stars score: 96/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    humidified co2 incubator - by Bioz Stars, 2019-10
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    Effect of neferine and DOX on the pro-inflammatory transcription factor, NFκB p65 and its downstream regulators, COX2 and TNF-α ( A ) Time dependent expression patterns of NFκB (nuclear extract) and COX2 and TNF-α (cytoplasmic extract) (0 h, 1 h, 3 h, 6 h, 12 h and 24 h). ( B ) DOX-induced nuclear translocation of NFκB, expression of COX2 and TNF-α was reduced by neferine treatment. Results were confirmed in the presence of NFκB inhibitor (QNZ). ( C ) Densitometry analysis of the protein bands of NFκB, COX2 and TNF-α. The results shown are mean ± s.d. of three individual experiments. * p

    Journal: Scientific Reports

    Article Title: Neferine ameliorates cardiomyoblast apoptosis induced by doxorubicin: possible role in modulating NADPH oxidase/ROS-mediated NFκB redox signaling cascade

    doi: 10.1038/s41598-017-12060-9

    Figure Lengend Snippet: Effect of neferine and DOX on the pro-inflammatory transcription factor, NFκB p65 and its downstream regulators, COX2 and TNF-α ( A ) Time dependent expression patterns of NFκB (nuclear extract) and COX2 and TNF-α (cytoplasmic extract) (0 h, 1 h, 3 h, 6 h, 12 h and 24 h). ( B ) DOX-induced nuclear translocation of NFκB, expression of COX2 and TNF-α was reduced by neferine treatment. Results were confirmed in the presence of NFκB inhibitor (QNZ). ( C ) Densitometry analysis of the protein bands of NFκB, COX2 and TNF-α. The results shown are mean ± s.d. of three individual experiments. * p

    Article Snippet: Cell culture flask was maintained at 37 °C in a humidified CO2 incubator (Nuaire make) with 5% CO2 supply.

    Techniques: Expressing, Translocation Assay