mant gtpγs 2 3 o n  (Jena Bioscience)


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  • 92
    Name:
    Mant GTPγS
    Description:

    Catalog Number:
    nu-209l
    Price:
    369.76
    Applications:
    Inhibition of AC5[1], ACs[2]
    Purity:
    ≥ 90 % (HPLC)
    Category:
    Nucleotides Nucleosides
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    Structured Review

    Jena Bioscience mant gtpγs 2 3 o n

    https://www.bioz.com/result/mant gtpγs 2 3 o n/product/Jena Bioscience
    Average 92 stars, based on 76 article reviews
    Price from $9.99 to $1999.99
    mant gtpγs 2 3 o n - by Bioz Stars, 2020-08
    92/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Phosphorylation decelerates conformational dynamics in bacterial translation elongation factors
    Article Snippet: .. EF-Tu or pEF-TuT382 at a concentration of 1 μM was rapidly mixed with a concentration range (5 to 25 μM) of MANT-labeled nucleotides (MANT-GDP and MANT-GTPγS; Jena Bioscience). .. MANT-labeled nucleotides were excited indirectly at 280 nm using resonance energy transfer (FRET) between tryptophan and the MANT-labeled nucleotide, and the change in fluorescence was monitored through a 405-nm cutoff filter.

    Fluorescence:

    Article Title: GTP-dependent packing of a three-helix bundle is required for atlastin-mediated fusion
    Article Snippet: .. Atlastin protein, buffer [25 mM Hepes-KOH (pH 7.4), 100 mM KCl, 10% glycerol, and 2 mM 2-mercaptoethanol], 5 mM Mg2+ , and 1 μM Mant-GTPγS [2′/3′-O-( N -Methyl-anthraniloyl)-guanosine-5′-(γ-thio)-triphosphate; Jena Biosciences] were mixed in a black 96-well plate and Mant fluorescence (λEx = 366 nm, λEm = 435 nm) measured at 25 °C in a TECAN Infinite M200 fluorimeter every 2 min for 20 min and averaged. .. The absolute fluorescence intensities were plotted vs. protein concentration and curve fitted in KaleidaGraph (Synergy Software) to determine an equilibrium Kd according to the following equation ( ): where Fmin is the fluorescence minimum, Fmax is the fluorescence maximum, A 0 is the concentration of protein in μM, B 0 is the concentration of Mant-GTPγS in μM, and Kd is the equilibrium binding constant in μM.

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    Jena Bioscience mgcl2
    Mgcl2, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 89/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgcl2/product/Jena Bioscience
    Average 89 stars, based on 75 article reviews
    Price from $9.99 to $1999.99
    mgcl2 - by Bioz Stars, 2020-08
    89/100 stars
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