datp  (Jena Bioscience)


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  • 85
    Name:
    7 Deaza dATP
    Description:

    Catalog Number:
    nu-1175l
    Price:
    343.4
    Category:
    Nucleotides Nucleosides
    Size:
    5 x 100 µl
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    Structured Review

    Jena Bioscience datp
    Synthesis of <t>7-deaza-7-modified</t> <t>dATP.</t>

    https://www.bioz.com/result/datp/product/Jena Bioscience
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    datp - by Bioz Stars, 2021-03
    85/100 stars

    Images

    1) Product Images from "Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases"

    Article Title: Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkp845

    Synthesis of 7-deaza-7-modified dATP.
    Figure Legend Snippet: Synthesis of 7-deaza-7-modified dATP.

    Techniques Used: Modification

    Related Articles

    Synthesized:

    Article Title: Formation of noncanonical DNA structures mediated by human ORC4, a protein component of the origin recognition complex.
    Article Snippet: Many genomic sequences, DNA replication origins included, contain specific structural motifs prone to alternative base pairing. .. Many genomic sequences, DNA replication origins included, contain specific structural motifs prone to alternative base pairing. ..

    Magnetic Beads:

    Article Title: Direct voltammetric analysis of DNA modified with enzymatically incorporated 7-deazapurines.
    Article Snippet: Nucleic acids studies use 7-deazaguanine (G*) and 7-deazaadenine (A*) as analogues of natural purine bases incapable of forming Hoogsteen base pairs, which prevents them from being involved in DNA triplexes and tetraplexes. .. Nucleic acids studies use 7-deazaguanine (G*) and 7-deazaadenine (A*) as analogues of natural purine bases incapable of forming Hoogsteen base pairs, which prevents them from being involved in DNA triplexes and tetraplexes. ..

    SYBR Green Assay:

    Article Title: Direct voltammetric analysis of DNA modified with enzymatically incorporated 7-deazapurines.
    Article Snippet: Nucleic acids studies use 7-deazaguanine (G*) and 7-deazaadenine (A*) as analogues of natural purine bases incapable of forming Hoogsteen base pairs, which prevents them from being involved in DNA triplexes and tetraplexes. .. Nucleic acids studies use 7-deazaguanine (G*) and 7-deazaadenine (A*) as analogues of natural purine bases incapable of forming Hoogsteen base pairs, which prevents them from being involved in DNA triplexes and tetraplexes. ..

    Modification:

    Article Title: Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases
    Article Snippet: .. Kinetics of cleavage of unmodified and modifided DNA by KpnI and SacI The reaction mixture (70 μl) contained Vent(exo-) DNA polymerase (New England Biolabs, 0.1 U), natural dNTPs (dGTP, dCTP and TTP, Fermentas, 0.2 mM), modified surrogates of dATP (derivatives of 7-deaza-dATP (7-deaza dATP, Jena Bioscience), 0.2 mM, 8-modified dATP 1 mM), primer (Sigma-Aldrich oligoes, sequence see and , 0.15 μM), template (Sigma-Aldrich oligoes, sequence see and , 0.225 μM) in 1 × ThermoPol reaction buffer. .. Primer was labeled by use of [γ32P]-ATP according to standard techniques.

    Sequencing:

    Article Title: Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases
    Article Snippet: .. Kinetics of cleavage of unmodified and modifided DNA by KpnI and SacI The reaction mixture (70 μl) contained Vent(exo-) DNA polymerase (New England Biolabs, 0.1 U), natural dNTPs (dGTP, dCTP and TTP, Fermentas, 0.2 mM), modified surrogates of dATP (derivatives of 7-deaza-dATP (7-deaza dATP, Jena Bioscience), 0.2 mM, 8-modified dATP 1 mM), primer (Sigma-Aldrich oligoes, sequence see and , 0.15 μM), template (Sigma-Aldrich oligoes, sequence see and , 0.225 μM) in 1 × ThermoPol reaction buffer. .. Primer was labeled by use of [γ32P]-ATP according to standard techniques.

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  • 85
    Jena Bioscience datp
    Synthesis of <t>7-deaza-7-modified</t> <t>dATP.</t>
    Datp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/datp/product/Jena Bioscience
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    datp - by Bioz Stars, 2021-03
    85/100 stars
      Buy from Supplier

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    Synthesis of 7-deaza-7-modified dATP.

    Journal: Nucleic Acids Research

    Article Title: Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases

    doi: 10.1093/nar/gkp845

    Figure Lengend Snippet: Synthesis of 7-deaza-7-modified dATP.

    Article Snippet: Kinetics of cleavage of unmodified and modifided DNA by KpnI and SacI The reaction mixture (70 μl) contained Vent(exo-) DNA polymerase (New England Biolabs, 0.1 U), natural dNTPs (dGTP, dCTP and TTP, Fermentas, 0.2 mM), modified surrogates of dATP (derivatives of 7-deaza-dATP (7-deaza dATP, Jena Bioscience), 0.2 mM, 8-modified dATP 1 mM), primer (Sigma-Aldrich oligoes, sequence see and , 0.15 μM), template (Sigma-Aldrich oligoes, sequence see and , 0.225 μM) in 1 × ThermoPol reaction buffer.

    Techniques: Modification